KR20210153670A - 변이체 효소를 통한 스테비올 글리코사이드 레바우디오사이드 i의 생합성 생산 - Google Patents

변이체 효소를 통한 스테비올 글리코사이드 레바우디오사이드 i의 생합성 생산 Download PDF

Info

Publication number
KR20210153670A
KR20210153670A KR1020217037306A KR20217037306A KR20210153670A KR 20210153670 A KR20210153670 A KR 20210153670A KR 1020217037306 A KR1020217037306 A KR 1020217037306A KR 20217037306 A KR20217037306 A KR 20217037306A KR 20210153670 A KR20210153670 A KR 20210153670A
Authority
KR
South Korea
Prior art keywords
leu
glu
ser
val
gly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020217037306A
Other languages
English (en)
Korean (ko)
Inventor
구오홍 마오
마이클 배튼
샤오단 위
Original Assignee
코나겐 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 코나겐 인크. filed Critical 코나겐 인크.
Publication of KR20210153670A publication Critical patent/KR20210153670A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Mycology (AREA)
KR1020217037306A 2018-03-12 2020-07-09 변이체 효소를 통한 스테비올 글리코사이드 레바우디오사이드 i의 생합성 생산 Ceased KR20210153670A (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201862641590P 2018-03-12 2018-03-12
US201862682260P 2018-06-08 2018-06-08
US201862695252P 2018-07-09 2018-07-09
US16/506,892 2019-07-09
US16/506,892 US11447808B2 (en) 2018-03-12 2019-07-09 Biosynthetic production of steviol glycoside rebaudioside I via variant enzymes
US16/679,032 2019-11-08
US16/679,032 US10883130B2 (en) 2018-03-12 2019-11-08 Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N
PCT/US2020/041394 WO2021007423A1 (en) 2018-03-12 2020-07-09 Biosynthetic production of steviol glycoside rebaudioside i via variant enzymes

Publications (1)

Publication Number Publication Date
KR20210153670A true KR20210153670A (ko) 2021-12-17

Family

ID=67908465

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020217037306A Ceased KR20210153670A (ko) 2018-03-12 2020-07-09 변이체 효소를 통한 스테비올 글리코사이드 레바우디오사이드 i의 생합성 생산

Country Status (8)

Country Link
US (4) US11447808B2 (enExample)
EP (2) EP3765627A4 (enExample)
JP (3) JP7365707B2 (enExample)
KR (1) KR20210153670A (enExample)
CN (1) CN111868252B (enExample)
BR (1) BR112021021828A2 (enExample)
MX (1) MX2021013721A (enExample)
WO (2) WO2019178116A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023121427A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법
WO2026101249A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7365707B2 (ja) 2018-03-12 2023-10-20 コナゲン インコーポレイテッド ステビオール配糖体レバウジオシドｊ及びレバウジオシドｎの生合成による製造
JP7318989B2 (ja) * 2019-03-29 2023-08-01 コナゲン インコーポレイテッド Ｕｄｐ－ラムノースの生合成生産
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
US20240263152A1 (en) * 2021-06-01 2024-08-08 Abiochem Biotechnology Co., Ltd. Glycosyltransferase and application thereof
WO2026101250A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도
CN119842657B (zh) * 2025-03-18 2025-05-27 青岛奔月生物技术有限公司 蔗糖合酶突变体及其应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL128193A (en) * 1999-01-22 2008-03-20 Yeda Res & Dev Rhamnosyl-transferase gene and uses thereof
US20060143729A1 (en) * 2004-06-30 2006-06-29 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
US8791253B2 (en) * 2006-06-19 2014-07-29 The Coca-Cola Company Rebaudioside A composition and method for purifying rebaudioside A
AU2010267963B2 (en) * 2009-07-02 2015-09-24 Merz Pharma Gmbh & Co. Kgaa Neurotoxins exhibiting shortened biological activity
EP2742142B2 (en) * 2011-08-08 2020-03-25 Evolva SA Recombinant production of steviol glycosides
MX395285B (es) * 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
CA2899276C (en) * 2013-02-06 2022-11-01 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
WO2016036578A1 (en) * 2014-09-02 2016-03-10 Purecircle Sdn Bhd Stevia extracts
KR102557670B1 (ko) 2014-10-03 2023-07-20 코나겐 인크. 비칼로리 감미료 및 합성 방법
US10463062B2 (en) 2014-11-05 2019-11-05 Manus Bio Inc. Microbial production of steviol glycosides
AU2016250184B2 (en) * 2015-04-14 2020-07-30 Conagen Inc. Production of non-caloric sweeteners using engineered whole-cell catalysts
AU2016271125A1 (en) * 2015-05-29 2017-12-21 Cargill, Incorporated Heat treatment to produce glycosides
US20190062796A1 (en) 2015-12-10 2019-02-28 Evolva Sa Production of steviol glycosides in recombinant hosts
JP6771937B2 (ja) * 2016-04-21 2020-10-21 キヤノンメディカルシステムズ株式会社 医用画像表示装置および医用画像管理システム
WO2018031955A2 (en) * 2016-08-12 2018-02-15 Amyris, Inc. Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
JP7177048B2 (ja) * 2016-10-14 2022-11-22 コナゲン インコーポレイテッド ステビオール配糖体の生合成製造およびそのためのプロセス
WO2018072211A1 (zh) 2016-10-21 2018-04-26 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙j的方法
JP7365707B2 (ja) 2018-03-12 2023-10-20 コナゲン インコーポレイテッド ステビオール配糖体レバウジオシドｊ及びレバウジオシドｎの生合成による製造
AU2019304965B2 (en) * 2018-07-16 2025-04-24 Manus Bio, Inc. Production of steviol glycosides through whole cell biotransformation
JP7318989B2 (ja) * 2019-03-29 2023-08-01 コナゲン インコーポレイテッド Ｕｄｐ－ラムノースの生合成生産
WO2020264179A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
EP4044823A4 (en) * 2019-10-16 2024-04-24 Sweegen, Inc. STEVIOL GLYCOSIDE FORMULATIONS FOR FOOD AND BEVERAGES

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023121427A1 (ko) * 2021-12-24 2023-06-29 주식회사 삼양사 당전이 효소 변이체 및 이를 이용한 스테비올 배당체의 제조방법
WO2026101249A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도

Also Published As

Publication number Publication date
BR112021021828A2 (pt) 2022-01-04
WO2019178116A1 (en) 2019-09-19
MX2021013721A (es) 2021-12-10
US11447808B2 (en) 2022-09-20
EP3997233A4 (en) 2023-08-23
US10883130B2 (en) 2021-01-05
US20200002742A1 (en) 2020-01-02
US20220411843A1 (en) 2022-12-29
WO2019178116A8 (en) 2019-10-10
EP3997233A1 (en) 2022-05-18
JP7305213B2 (ja) 2023-07-10
EP3765627A4 (en) 2021-10-13
WO2021007423A1 (en) 2021-01-14
US20200087695A1 (en) 2020-03-19
JP2021515555A (ja) 2021-06-24
US20210189448A1 (en) 2021-06-24
JP2022540306A (ja) 2022-09-15
CN111868252B (zh) 2025-06-03
JP2023126903A (ja) 2023-09-12
CN111868252A (zh) 2020-10-30
US11441165B2 (en) 2022-09-13
JP7365707B2 (ja) 2023-10-20
EP3765627A1 (en) 2021-01-20

Similar Documents

Publication Publication Date Title
JP7305213B2 (ja) 変異体酵素を介するステビオール配糖体レバウシオシドｉの生合成的産生
RU2741103C2 (ru) Рекомбинантное получение стевиол-гликозидов
KR102554790B1 (ko) 레바우디오시드 e로부터 스테비올 글리코시드 레바우디오시드 d4의 생합성 제조
CN110809408B (zh) 通过β-葡糖苷酶水解甜菊醇糖苷
US20220205007A1 (en) Biosynthetic production of steviol glycoside rebaudioside i via variant enzymes
HK1230659B (en) Recombinant production of steviol glycosides
HK1230659A1 (en) Recombinant production of steviol glycosides

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20211115

Patent event code: PA01051R01D

Comment text: International Patent Application

A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20211119

Comment text: Request for Examination of Application

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20231227

Patent event code: PE09021S01D

PE0601 Decision on rejection of patent

Patent event date: 20240704

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D