BR112021021828A2 - Método para sintetizar rebaudiosídeo i e mutante ugt76g1 - Google Patents
Método para sintetizar rebaudiosídeo i e mutante ugt76g1Info
- Publication number
- BR112021021828A2 BR112021021828A2 BR112021021828A BR112021021828A BR112021021828A2 BR 112021021828 A2 BR112021021828 A2 BR 112021021828A2 BR 112021021828 A BR112021021828 A BR 112021021828A BR 112021021828 A BR112021021828 A BR 112021021828A BR 112021021828 A2 BR112021021828 A2 BR 112021021828A2
- Authority
- BR
- Brazil
- Prior art keywords
- rebaudioside
- synthesize
- ugt76g1 mutant
- ugt76g1
- mutant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
- A23L27/36—Terpene glycosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Mycology (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862641590P | 2018-03-12 | 2018-03-12 | |
| US201862682260P | 2018-06-08 | 2018-06-08 | |
| US201862695252P | 2018-07-09 | 2018-07-09 | |
| US16/506,892 US11447808B2 (en) | 2018-03-12 | 2019-07-09 | Biosynthetic production of steviol glycoside rebaudioside I via variant enzymes |
| US16/679,032 US10883130B2 (en) | 2018-03-12 | 2019-11-08 | Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N |
| PCT/US2020/041394 WO2021007423A1 (en) | 2018-03-12 | 2020-07-09 | Biosynthetic production of steviol glycoside rebaudioside i via variant enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112021021828A2 true BR112021021828A2 (pt) | 2022-01-04 |
Family
ID=67908465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021021828A BR112021021828A2 (pt) | 2018-03-12 | 2020-07-09 | Método para sintetizar rebaudiosídeo i e mutante ugt76g1 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US11447808B2 (enExample) |
| EP (2) | EP3765627A4 (enExample) |
| JP (3) | JP7365707B2 (enExample) |
| KR (1) | KR20210153670A (enExample) |
| CN (1) | CN111868252B (enExample) |
| BR (1) | BR112021021828A2 (enExample) |
| MX (1) | MX2021013721A (enExample) |
| WO (2) | WO2019178116A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7365707B2 (ja) | 2018-03-12 | 2023-10-20 | コナゲン インコーポレイテッド | ステビオール配糖体レバウジオシドｊ及びレバウジオシドｎの生合成による製造 |
| JP7318989B2 (ja) * | 2019-03-29 | 2023-08-01 | コナゲン インコーポレイテッド | Ｕｄｐ－ラムノースの生合成生産 |
| CN112080480B (zh) * | 2019-06-14 | 2023-01-03 | 中国科学院分子植物科学卓越创新中心 | 糖基转移酶突变体及其应用 |
| US20240263152A1 (en) * | 2021-06-01 | 2024-08-08 | Abiochem Biotechnology Co., Ltd. | Glycosyltransferase and application thereof |
| CN118489006A (zh) * | 2021-12-24 | 2024-08-13 | 株式会社三养社 | 糖基转移酶变体和使用其制备甜菊糖苷的方法 |
| WO2026101250A1 (ko) * | 2024-11-06 | 2026-05-15 | 씨제이제일제당 (주) | 신규한 당전이 효소 및 이의 용도 |
| WO2026101249A1 (ko) * | 2024-11-06 | 2026-05-15 | 씨제이제일제당 (주) | 신규한 당전이 효소 및 이의 용도 |
| CN119842657B (zh) * | 2025-03-18 | 2025-05-27 | 青岛奔月生物技术有限公司 | 蔗糖合酶突变体及其应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL128193A (en) * | 1999-01-22 | 2008-03-20 | Yeda Res & Dev | Rhamnosyl-transferase gene and uses thereof |
| US20060143729A1 (en) * | 2004-06-30 | 2006-06-29 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| US8791253B2 (en) * | 2006-06-19 | 2014-07-29 | The Coca-Cola Company | Rebaudioside A composition and method for purifying rebaudioside A |
| AU2010267963B2 (en) * | 2009-07-02 | 2015-09-24 | Merz Pharma Gmbh & Co. Kgaa | Neurotoxins exhibiting shortened biological activity |
| EP2742142B2 (en) * | 2011-08-08 | 2020-03-25 | Evolva SA | Recombinant production of steviol glycosides |
| MX395285B (es) * | 2012-05-22 | 2025-03-25 | Purecircle Sdn Bhd | Glucosidos de esteviol de alta pureza. |
| US9752174B2 (en) * | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| CA2899276C (en) * | 2013-02-06 | 2022-11-01 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| EP3683315A1 (en) | 2014-08-19 | 2020-07-22 | Purecircle SDN BHD | Method for preparing rebaudioside m |
| WO2016036578A1 (en) * | 2014-09-02 | 2016-03-10 | Purecircle Sdn Bhd | Stevia extracts |
| KR102557670B1 (ko) | 2014-10-03 | 2023-07-20 | 코나겐 인크. | 비칼로리 감미료 및 합성 방법 |
| US10463062B2 (en) | 2014-11-05 | 2019-11-05 | Manus Bio Inc. | Microbial production of steviol glycosides |
| AU2016250184B2 (en) * | 2015-04-14 | 2020-07-30 | Conagen Inc. | Production of non-caloric sweeteners using engineered whole-cell catalysts |
| AU2016271125A1 (en) * | 2015-05-29 | 2017-12-21 | Cargill, Incorporated | Heat treatment to produce glycosides |
| US20190062796A1 (en) | 2015-12-10 | 2019-02-28 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| JP6771937B2 (ja) * | 2016-04-21 | 2020-10-21 | キヤノンメディカルシステムズ株式会社 | 医用画像表示装置および医用画像管理システム |
| WO2018031955A2 (en) * | 2016-08-12 | 2018-02-15 | Amyris, Inc. | Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides |
| JP7177048B2 (ja) * | 2016-10-14 | 2022-11-22 | コナゲン インコーポレイテッド | ステビオール配糖体の生合成製造およびそのためのプロセス |
| WO2018072211A1 (zh) | 2016-10-21 | 2018-04-26 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙j的方法 |
| JP7365707B2 (ja) | 2018-03-12 | 2023-10-20 | コナゲン インコーポレイテッド | ステビオール配糖体レバウジオシドｊ及びレバウジオシドｎの生合成による製造 |
| AU2019304965B2 (en) * | 2018-07-16 | 2025-04-24 | Manus Bio, Inc. | Production of steviol glycosides through whole cell biotransformation |
| JP7318989B2 (ja) * | 2019-03-29 | 2023-08-01 | コナゲン インコーポレイテッド | Ｕｄｐ－ラムノースの生合成生産 |
| WO2020264179A1 (en) * | 2019-06-25 | 2020-12-30 | Manus Bio, Inc. | Uridine diphosphate-dependent glycosyltransferase enzyme |
| EP4044823A4 (en) * | 2019-10-16 | 2024-04-24 | Sweegen, Inc. | STEVIOL GLYCOSIDE FORMULATIONS FOR FOOD AND BEVERAGES |
-
2019
- 2019-03-12 JP JP2020546338A patent/JP7365707B2/ja active Active
- 2019-03-12 CN CN201980018867.0A patent/CN111868252B/zh active Active
- 2019-03-12 WO PCT/US2019/021876 patent/WO2019178116A1/en not_active Ceased
- 2019-03-12 EP EP19767823.8A patent/EP3765627A4/en active Pending
- 2019-07-09 US US16/506,892 patent/US11447808B2/en active Active
- 2019-11-08 US US16/679,032 patent/US10883130B2/en active Active
-
2020
- 2020-07-09 BR BR112021021828A patent/BR112021021828A2/pt unknown
- 2020-07-09 KR KR1020217037306A patent/KR20210153670A/ko not_active Ceased
- 2020-07-09 WO PCT/US2020/041394 patent/WO2021007423A1/en not_active Ceased
- 2020-07-09 MX MX2021013721A patent/MX2021013721A/es unknown
- 2020-07-09 JP JP2021571495A patent/JP7305213B2/ja active Active
- 2020-07-09 EP EP20836091.7A patent/EP3997233A4/en active Pending
- 2020-12-02 US US17/109,473 patent/US11441165B2/en active Active
-
2022
- 2022-08-12 US US17/819,423 patent/US20220411843A1/en active Pending
-
2023
- 2023-07-04 JP JP2023110059A patent/JP2023126903A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019178116A1 (en) | 2019-09-19 |
| MX2021013721A (es) | 2021-12-10 |
| US11447808B2 (en) | 2022-09-20 |
| EP3997233A4 (en) | 2023-08-23 |
| US10883130B2 (en) | 2021-01-05 |
| US20200002742A1 (en) | 2020-01-02 |
| US20220411843A1 (en) | 2022-12-29 |
| WO2019178116A8 (en) | 2019-10-10 |
| EP3997233A1 (en) | 2022-05-18 |
| JP7305213B2 (ja) | 2023-07-10 |
| EP3765627A4 (en) | 2021-10-13 |
| WO2021007423A1 (en) | 2021-01-14 |
| US20200087695A1 (en) | 2020-03-19 |
| JP2021515555A (ja) | 2021-06-24 |
| US20210189448A1 (en) | 2021-06-24 |
| KR20210153670A (ko) | 2021-12-17 |
| JP2022540306A (ja) | 2022-09-15 |
| CN111868252B (zh) | 2025-06-03 |
| JP2023126903A (ja) | 2023-09-12 |
| CN111868252A (zh) | 2020-10-30 |
| US11441165B2 (en) | 2022-09-13 |
| JP7365707B2 (ja) | 2023-10-20 |
| EP3765627A1 (en) | 2021-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2021013721A (es) | Produccion biosintetica del glucosido de esteviol rebaudiosido i a traves de enzimas variantes. | |
| NZ725866A (en) | A non-caloric sweetener | |
| MX2021005862A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| MX2021001260A (es) | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. | |
| BR112018074999A2 (pt) | glicosiltransferase dependente de udp para a produção de alta eficiência de rebaudiosídeo | |
| MY205372A (en) | Imp-producing microorganism and method of producing imp using the same | |
| MY204735A (en) | Production of steviol glycosides through whole cell biotransformation | |
| BR112018073662A2 (pt) | produção de glicosídeos de esteviol em hospedeiros recombinantes | |
| MX2020001903A (es) | Novedoso polipeptido y procedimiento de produccion de imp mediante el uso del mismo. | |
| PY21104309A (es) | Composición que comprende estevia glucosilada y que tiene una calidad de dulce mejorada | |
| MX394842B (es) | Glicosidos de esteviol altamente solubles. | |
| MX378831B (es) | Edulcorantes no caloricos y metodos para sintetizar | |
| BR112019007504A2 (pt) | produção biossintética de glicosídeos de steviol e seus processos | |
| BR112021015766A2 (pt) | Método de biossíntese da diosmina e/ou da hesperidina em um microrganismo | |
| MX2018015517A (es) | Glicosilacion enzimatica de glucosidos de esteviol y otros compuestos con glucosa-1-fosfato. | |
| BR112018011611A2 (pt) | produção de glicosídeos de esteviol em hospedeiros recombinantes | |
| MX2024014263A (es) | Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e | |
| PY2250533A (es) | Nueva uridina difosfato glucosiltransferasa y uso del mismo | |
| BR112021019470A2 (pt) | Produção biossintética de udp-ramnose | |
| EP3954780A4 (en) | COMPOSITION FOR THE PREPARATION OF GLUCOSE-TRANSFERRING STEVIOL GLYCOSIDE WITH A GLUCOSE-TRANSFERRING ENZYME AND PROCESS FOR THE PREPARATION OF GLUCOSE-TRANSFERRING STEVIOL GLYCOSIDE USING THEREOF | |
| MX2020003234A (es) | Metodos para preparar glucosidos de esteviol y usos de los mismos. | |
| PY2018536A (es) | Composición que comprende glucósido de esteviol transfructosilado | |
| MX2019004629A (es) | Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico. | |
| ZA202107583B (en) | Cytidine derivatives and methods of forming cytidine derivatives | |
| PY2015735A (es) | Composición que comprende esteviósido transglucosilado y rebaudiósido a transglucosilado |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |