CN111868252B - 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 - Google Patents

甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 Download PDF

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CN111868252B
CN111868252B CN201980018867.0A CN201980018867A CN111868252B CN 111868252 B CN111868252 B CN 111868252B CN 201980018867 A CN201980018867 A CN 201980018867A CN 111868252 B CN111868252 B CN 111868252B
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seq
rebaudioside
reb
recombinant polypeptide
sequence
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CN111868252A (zh
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毛国红
M·巴腾
P·宏特
余晓丹
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Conagen Inc
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Conagen Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Mycology (AREA)
CN201980018867.0A 2018-03-12 2019-03-12 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 Active CN111868252B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862641590P 2018-03-12 2018-03-12
US62/641590 2018-03-12
US201862682260P 2018-06-08 2018-06-08
US62/682260 2018-06-08
US201862695252P 2018-07-09 2018-07-09
US62/695252 2018-07-09
PCT/US2019/021876 WO2019178116A1 (en) 2018-03-12 2019-03-12 Biosynthetic production of steviol glycosides rebaudioside j and rebaudioside n

Publications (2)

Publication Number Publication Date
CN111868252A CN111868252A (zh) 2020-10-30
CN111868252B true CN111868252B (zh) 2025-06-03

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Country Status (8)

Country Link
US (4) US11447808B2 (enExample)
EP (2) EP3765627A4 (enExample)
JP (3) JP7365707B2 (enExample)
KR (1) KR20210153670A (enExample)
CN (1) CN111868252B (enExample)
BR (1) BR112021021828A2 (enExample)
MX (1) MX2021013721A (enExample)
WO (2) WO2019178116A1 (enExample)

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JP7365707B2 (ja) 2018-03-12 2023-10-20 コナゲン インコーポレイテッド ステビオール配糖体レバウジオシドｊ及びレバウジオシドｎの生合成による製造
JP7318989B2 (ja) * 2019-03-29 2023-08-01 コナゲン インコーポレイテッド Ｕｄｐ－ラムノースの生合成生産
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
US20240263152A1 (en) * 2021-06-01 2024-08-08 Abiochem Biotechnology Co., Ltd. Glycosyltransferase and application thereof
CN118489006A (zh) * 2021-12-24 2024-08-13 株式会社三养社 糖基转移酶变体和使用其制备甜菊糖苷的方法
WO2026101250A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도
WO2026101249A1 (ko) * 2024-11-06 2026-05-15 씨제이제일제당 (주) 신규한 당전이 효소 및 이의 용도
CN119842657B (zh) * 2025-03-18 2025-05-27 青岛奔月生物技术有限公司 蔗糖合酶突变体及其应用

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WO2018031955A2 (en) * 2016-08-12 2018-02-15 Amyris, Inc. Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

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US8791253B2 (en) * 2006-06-19 2014-07-29 The Coca-Cola Company Rebaudioside A composition and method for purifying rebaudioside A
AU2010267963B2 (en) * 2009-07-02 2015-09-24 Merz Pharma Gmbh & Co. Kgaa Neurotoxins exhibiting shortened biological activity
EP2742142B2 (en) * 2011-08-08 2020-03-25 Evolva SA Recombinant production of steviol glycosides
MX395285B (es) * 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
CA2899276C (en) * 2013-02-06 2022-11-01 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
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EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
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WO2016073740A1 (en) * 2014-11-05 2016-05-12 Manus Biosynthesis, Inc. Microbial production of steviol glycosides
WO2018031955A2 (en) * 2016-08-12 2018-02-15 Amyris, Inc. Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides

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BR112021021828A2 (pt) 2022-01-04
WO2019178116A1 (en) 2019-09-19
MX2021013721A (es) 2021-12-10
US11447808B2 (en) 2022-09-20
EP3997233A4 (en) 2023-08-23
US10883130B2 (en) 2021-01-05
US20200002742A1 (en) 2020-01-02
US20220411843A1 (en) 2022-12-29
WO2019178116A8 (en) 2019-10-10
EP3997233A1 (en) 2022-05-18
JP7305213B2 (ja) 2023-07-10
EP3765627A4 (en) 2021-10-13
WO2021007423A1 (en) 2021-01-14
US20200087695A1 (en) 2020-03-19
JP2021515555A (ja) 2021-06-24
US20210189448A1 (en) 2021-06-24
KR20210153670A (ko) 2021-12-17
JP2022540306A (ja) 2022-09-15
JP2023126903A (ja) 2023-09-12
CN111868252A (zh) 2020-10-30
US11441165B2 (en) 2022-09-13
JP7365707B2 (ja) 2023-10-20
EP3765627A1 (en) 2021-01-20

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