KR20210153178A - Composition for promoting absorption of collagen and synthesis of collagen - Google Patents

Abstract

The present invention relates to a collagen composition that can be used in food, cosmetics or feed. To this end, provided is a composition promoting the absorption and synthesis of collagen, which is effective in absorbing and synthesizing collagen and also has good preservability or stability. The collagen composition of the present invention comprises: purified water; collagen; hyaluronic acid; Beta vulgaris subsp. vulgaris Conditiva Group concentrate; Malpighia emarginata concentrate; taurine; essential amino acids; L-arginine; complexed Scutellaria baicalensis extract; erythritol; natural Punica granatum fragrance; enzyme-treated stevia; citric acid anhydrous; sodium citrate; Punica granatum concentrate; and sevenberry (blueberry, cranberry, acaiberry, blackberry, raspberry, strawberry, and blackcurrant) concentrate.

Description

A composition that promotes the absorption of collagen and the synthesis of collagen

The present invention relates to a composition that promotes collagen absorption into the body and collagen synthesis in the body.

Collagen, also called hard protein or collagen, is widely distributed in multicellular animals such as invertebrates and vertebrates and is the most quantitatively found hard protein. In mammals, it accounts for about 1/3 of the total protein and is a hard protein with very strong fibrous tension, which constitutes the connective tissue of animals, and transmits force from tendons or ligaments without loss.

The basic structural unit of collagen is tropocollagen with a molecular weight of about 300,000. This molecule has a triple helix structure in which the three-stranded polypeptide chain is right-twisted. Tropocollagen molecules associate to form collagen fibers (collagen fibers), and each molecule is arranged one-quarter shift in the axial direction to form unique stripes at 64 nm intervals. becomes insoluble. However, if collagen is boiled for a long time in hot water, dilute acid, or dilute alkali, it is converted into water-soluble inducible protein gelatin.

It is known that the main function of collagen is the firmness of the skin, the resistance and bonding of tissues, and the support of cell adhesion. Such collagen is known to be closely related to the formation of wrinkles in the skin because the thickness of the skin becomes thinner due to aging and photoaging caused by UV irradiation.

As income levels rise and we enter an aging society, extensive research on skin aging has progressed, revealing the function of collagen on the skin. Among them, retinoic acid (retinoic acid), a collagen synthesis promoting substance, can be expected to have the effects of wrinkle improvement, elasticity enhancement, skin strengthening and wound healing by increasing the components of the dermal matrix when collagen metabolism is activated by promoting collagen synthesis. ), transforming growth factor (TGF), animal-derived placenta, betulinic acid, chlorella extract, etc. have been released as cosmetic compositions for skin strengthening, but many products have not received much attention.

In addition, there are many products that contain collagen as a cosmetic composition for skin protection, but it is pointed out that cosmetics applied to the skin surface have difficulty in dermal absorption of collagen, which is a polymer, so that it is difficult to expect a moisturizing effect on the skin.

Recently, the use of collagen peptides as health food, surgical or ophthalmic treatment or cosmetic additive is increasing. On the other hand, from a food, pharmaceutical, and cosmetic point of view, the stability of the product and absorption rate into the skin or body are important for these collagen peptides.

Korean Patent Publication No. 10-2017-0093694 (2017.08.16)

The present invention is a collagen composition that can be used in food, cosmetics, or feed, and is effective for the absorption and synthesis of collagen in the body, as well as a composition that promotes the absorption and synthesis of collagen with good preservation or stability and good texture. to provide.

The collagen composition of the present invention is purified water, collagen, hyaluronic acid, red beet concentrate, acerola concentrate, taurine, essential amino acids, L-arginine, complex golden extract, erythritol, natural pomegranate flavor, enzyme-treated stevia, citric anhydride (Citric Acid Anhydrous), trisodium citrate, pomegranate concentrate, and sevenberry concentrate.

The collagen composition comprises 50 to 52 parts by weight of purified water, 26 to 27 parts by weight of collagen, 0.4 to 0.5 parts by weight of hyaluronic acid, 0.6 to 0.7 parts by weight of red beet concentrate, 1.18 to 1.22 parts by weight of acerola concentrate, 1.6 to 1.7 parts by weight of taurine, Essential amino acid 0.09 to 0.11 parts by weight, L-arginine 0.035 to 0.039 parts by weight, complex golden extract 0.19 to 0.21 parts by weight, erythritol 10.8 to 11.2 parts by weight, natural pomegranate flavor 0.5 to 0.7 parts by weight, enzyme-treated stevia 0.198 to 0.022 parts by weight, It may contain 1.1 to 1.3 parts by weight of Citric Acid Anhydrous, 0.08 to 0.12 parts by weight of trisodium citrate, 2.9 to 3.1 parts by weight of pomegranate concentrate, and 1.9 to 2.1 parts by weight of sevenberry concentrate.

The collagen is nanosized to have a size of 1 nm to 10 nm and may be characterized as a collagen peptide having a molecular weight of 250 Da to 650 Da.

The collagen has a size of 6 nm to 8 nm, an average molecular weight of 500 Da, and may be characterized as a collagen peptide.

The collagen may be any one or more of KTTKS, GHK, AHK, and mixtures thereof.

The composition for promoting the absorption and synthesis of collagen of the present invention is not only effective for the absorption and synthesis of collagen in the body, but also has good preservation or stability, and has a good texture. Accordingly, skin aging can be suppressed, skin wrinkles can be improved, and skin moisture content can be improved by increasing the moisture content of the skin.

The composition of the present invention is effective in preventing anemia and lowering blood pressure because it is rich in vitamins, iron, potassium, fiber and beta-cyanin.

The composition of the present invention is rich in various minerals, such as ascorbic acid, becacarotene, niacin, thiamine, riboflavin, and flavonoid, and is effective in preventing allergic rhinitis or herpes virus infection.

Since the composition of the present invention contains taurine, it is effective for preventing various vascular diseases as well as for platelet aggregation in blood vessels.

Since the composition of the present invention contains L-arginine, it is effective in the treatment of hypertension and angina, antioxidant function, immune regulation, and wound healing.

Hereinafter, some embodiments of the present invention will be described in detail with reference to exemplary drawings. In adding reference numerals to the components of each drawing, it should be noted that the same components are given the same reference numerals as much as possible even though they are indicated on different drawings.

In the description of the embodiment of the present invention, if it is determined that a detailed description of a related known configuration or function interferes with the understanding of the embodiment of the present invention, the detailed description thereof will be omitted.

In addition, in describing the components of the embodiment of the present invention, terms such as first, second, A, B, (a), (b), etc. may be used. These terms are only for distinguishing the elements from other elements, and the essence, order, or order of the elements are not limited by the terms.

Hereinafter, a composition for promoting collagen absorption and collagen synthesis (hereinafter referred to as a 'collagen composition') according to an embodiment of the present invention will be described.

The collagen composition of the present invention contains purified water, collagen, hyaluronic acid, red beet concentrate, acerola concentrate, taurine, essential amino acids, L-arginine, complex golden extract, erythritol, natural pomegranate flavor, enzyme-treated stevia, anhydrous citric acid ( Citric Acid Anhydrous), trisodium citrate, pomegranate concentrate, and sevenberry concentrate.

Specifically, the collagen composition of the present invention contains 50 to 52 parts by weight of purified water, 26 to 27 parts by weight of collagen, 0.4 to 0.5 parts by weight of hyaluronic acid, 0.6 to 0.7 parts by weight of red beet concentrate, 1.18 to 1.22 parts by weight of acerola concentrate, 1.6 parts by weight of taurine. to 1.7 parts by weight, essential amino acids 0.09 to 0.11 parts by weight, L-arginine 0.035 to 0.039 parts by weight, complex golden extract 0.19 to 0.21 parts by weight, erythritol 10.8 to 11.2 parts by weight, natural pomegranate flavor 0.5 to 0.7 parts by weight, enzyme-treated stevia 0.198 to 0.022 parts by weight, citric acid anhydrous 1.1 to 1.3, trisodium citrate 0.08 to 0.12 parts by weight, pomegranate concentrate 2.9 to 3.1 parts by weight, and sevenberry concentrate 1.9 to 2.1 parts by weight.

The purified water refers to water from which all impurities such as dissolved ions, solid particles, microorganisms, organic matter, and dissolved gases contained in water are removed.

The collagen may be any one or more selected from the group consisting of animal collagen such as cow skin and pig skin, vegetable collagen such as fruits, vegetables, seeds, nuts, and mushrooms, and marine collagen such as seaweed, fish, fish scales, and fish skin.

The collagen may be a collagen peptide nanosized to have a size of 1 nm to 10 nm and a molecular weight of 250 Da to 650 Da.

Preferably, the collagen may be a collagen peptide having an average molecular weight of 500 Da and a size of 6 nm to 8 nm.

The collagen may be any one or more of KTTKS, GHK, AHK, and mixtures thereof.

The hyaluronic acid is a disaccharide polymer of glucuronic acid and N-acetyl glucosamine, and can form a gel in the collagen composition and prevent bacterial invasion.

The red beet concentrate is a concentrate in which the water content of red beets is reduced by 70% to 80%, preferably by 76% through an evaporation process.

With red beets rich in vitamins and iron, the collagen composition is effective in preventing anemia. In addition, since the root of red beet is rich in sugar, vitamin A, potassium, iron, fiber and beta cyanine, the collagen composition also has an effect of lowering blood pressure.

The acerola concentrate means a concentrate in which the moisture content of acerola is reduced by 76% to 86%, preferably by 82% through an evaporation process

Acerola has a high content of vitamin C, ascorbic acid, and becacarotene, and is rich in various minerals such as niacin, thiamine, riboflavin, flavonoids, and potassium. Accordingly, the collagen composition is effective not only in enhancing immunity and preventing aging, but also in preventing allergic rhinitis or herpes virus infection.

The taurine exerts an inhibitory action on the sympathetic nerves of the brain to help stabilize blood pressure and prevent stroke, increase the contractility when the contractility of the myocardium is lowered in a low calcium state of the heart, and conversely, increase the contractility in the case of high calcium It is effective in reducing arrhythmias and heart failure. In addition, taurine inhibits the production of low-density lipoprotein (LDL) cholesterol, which causes arteriosclerosis, angina, myocardial infarction, etc. It is effective in preventing various vascular diseases as well as platelet aggregation in blood vessels.

The taurine is produced by a reaction between ethylene oxide and sodium bisulfite to produce 2-hydroxyethanesulfonic acid, isethionic acid, and then through an ammoxidation reaction (aminolysis). Taurine may be synthesized, synthesized by reaction of aziridine and sulfurous acid, or may be synthesized by reaction of bromoethanesulfonic acid salt and ammonia.

The essential amino acids include isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine, in the body It is an amino acid that must be ingested from food because it is not synthesized in the body or is synthesized in a very small amount and is not sufficient to achieve physiological functions.

The L-arginine (C6H14N4O2) is an amino acid with strong basicity, and the aqueous solution becomes alkaline. Although it is a non-essential amino acid, it plays an important role in the urea synthesis reaction and is involved in the ornithine cycle. This L-arginine is effective in the treatment of hypertension and angina pectoris, antioxidant function, immune regulation, and wound healing.

The complex golden extract is used as a preservative, and contains 36.8% of golden concentrate, 27.2% of licorice concentrate, 10% of jujube concentrate, 7.2% of Astragalus concentrate, 10% of textrin, 2% of lactic acid, and 6.8% of glycerin.

The erythritol (erythritol) may be prepared through filtration, purification, crystallization, washing, and drying processes of fermentation broth such as yeast Moniliella pollinis or Trichosporonoides megachilensis.

Erythritol is a white crystalline powder, has no odor, is easily soluble in water, has excellent heat resistance, and has low hygroscopicity to easily form crystals. The sweetness of erythritol is about 70% to 80% of that of sugar and has a refreshing sweetness. Also, unlike other sweeteners, erythritol is not used as an energy source in the body and is mostly excreted, so it has the effect of having a low absorption rate in the body.

The enzyme-treated stevia (Enzymatically Modified Stevia Glucosyl Stevia) purifies α-glucosyl stevioside and α-glucosyl baudioside A, which are produced by the action of α-Glucosyltransferase (α-glucosyl transferase), etc. on the stevia extract The main component of sweetness is α-glucosylstevioside

The pomegranate concentrate is a concentrate in which the water content of the pomegranate is reduced by 72% to 88%, preferably by 80%, through an evaporation process.

Pomegranate contains 40% glucose and fructose, 1.5% citric acid, which gives a sour taste, and also contains water-soluble vitamins such as B1, B2, and niacin. And the pomegranate peel contains tannins and the seeds contain natural phytoestrogens that are good for menopausal disorders. Both the fruit and peel of the pomegranate are good for high blood pressure and arteriosclerosis prevention, and are effective for gynecological diseases and swelling.

The anhydrous citric acid (Anhydrous citric acid) is an organic acid that can be extracted from citrus or lemon in nature, and has sweetening, aroma, and sterilizing effects.

The trisodium citrate (Trisodium Citrate) is colorless and transparent crystals or white crystalline powder without odor and has a refreshing salty and bitter taste. And the trisodium citrate can adjust the pH of the collagen composition.

The seven berry concentrate means a concentrated mixture of blueberry, cranberry, strawberry, raspberry, acai berry, blackberry and blackcurrant.

Example 1_ Preparation of first collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 7.0235g, acerola concentrate 12.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 2.1060g, erythritol 115.8300g, natural pomegranate flavor 6.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 31.5900g, sevenberry concentrate 21.0600g 1053 g of the first collagen composition was prepared.

Here, the collagen is fish collagen, and collagen peptides having an average molecular weight of 500 Da and a size of 6 nm to 8 nm were used.

Comparative Example 1_ Preparation of the second collagen composition

A second collagen composition of 972.5929 was prepared in which 538.0198 g of purified water, 280.8351 g of collagen, 6.3180 g of natural pomegranate flavor, 115.8300 g of erythritol, and 31.5900 g of pomegranate concentrate were mixed.

Here, the collagen is fish collagen, and collagen peptides having an average molecular weight of 500 Da and a size of 6 nm to 8 nm were used.

Experimental Example 1_ Measurement of hydroxyproline peptide in blood

Women in their 40s, weighing 55 kg to 70 kg, were randomly divided into groups A and B, and 20 women were randomly divided into groups A and B. Group A consumed 100 g of the first collagen composition of Example 1 for 10 minutes, and group B Silver took 100 g of the second collagen composition of Comparative Example 1, and after 100 minutes, the average blood hydroxyproline peptides of groups A and B were measured. The results are shown in Table 1 below.

Serum hydroxyproline peptide (nmol/ml) group A 651.321 group B 322.293

It was confirmed that the absorption rate of the first collagen composition of Example 1 was 2.02 times higher than that of the second collagen composition of Comparative Example 1.

Experimental Example 2_ Measurement of skin moisture

Women in their 40s, weighing 55 kg to 70 kg, were randomly divided into 10 people each, group A and group B, and group A ingested 30 g of the first collagen composition of Example 1 for 30 days, group B The second collagen composition of Comparative Example 1 was ingested 30 g each for 30 days. The average of % of increased moisturizing power was confirmed by measuring skin moisture before ingestion and after ingestion for 30 days. Skin moisture was measured using a skin moisture meter (my-808s, Scala Co., Ltd. Made in Japan). The results are shown in Table 2 below.

skin moisturizing effect group A 35.3% group B 14.3%

It was confirmed that the skin moisturizing effect of the first collagen composition of Example 1 was 21% higher than the skin moisturizing effect of the second collagen composition of Comparative Example 1.

Experimental Example 3_ Skin elasticity measurement

Women in their 40s, weighing 55 kg to 70 kg, were randomly divided into 10 people each, group A and group B, and group A ingested 30 g of the first collagen composition of Example 1 for 30 days, group B The second collagen composition of Comparative Example 1 was ingested 30 g each for 30 days. The average of the percentage of increased elasticity was confirmed by measuring skin elasticity before and after ingestion for 30 days. Skin elasticity was measured using a cutometer (SEM474, Courage+Khazaka electronic GmbH Germany). The results are shown in Table 3 below.

skin elasticity effect group A 43.2% group B 15.3%

It was confirmed that the skin elasticity effect of the first collagen composition of Example 1 was 27.9% higher than the skin elasticity effect of the second collagen composition of Comparative Example 1.

The results of Experimental Example 2 and Experimental Example 3 show that minerals such as silicon (Si), iron (Fe), copper (Cu), which are abundant in red beets contained in the first collagen composition of Example 1, are important for collagen synthesis It acts as a booster (Collagen Booster), which is due to increasing collagen in the skin.

Experimental Example 4_Storage of food

By using the first collagen composition of Example 1 and the second collagen composition of Comparative Example 1, changes were confirmed for Baekseolgi, tofu, white bread, garaetteok, and agar. In order to confirm the change in food, the first collagen composition of Example 1 and the second collagen composition of Comparative Example 1 were mixed or sprayed with 1.2 to 2.3 wt% of each of the ingredients or cooked food of the cooking process after 24 hours at room temperature , degree of hardening, discoloration, etc. were compared through visual observation and sensory examination. The results are shown in Table 4 below, where A is very good, B is good, C is average, D is bad, and F is very poor.

division Application example Snow White tofu bread Garae Rice Cake amber Example 1 B B A A A Comparative Example 1 C C C C C

As can be seen in Table 4, it can be seen that the first collagen composition of Example 1 improves the storage properties of food than the second collagen composition of Comparative Example 1.

Comparative Example 2_ Preparation of the third collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 4.0235g, acerola concentrate 6.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 2.1060g, erythritol 115.8300g, natural pomegranate flavor 6.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 31.5900g, sevenberry concentrate 21.0600g 1044 g of the third collagen composition was prepared.

Comparative Example 3_Preparation of the fourth collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 7.0235g, acerola concentrate 12.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 2.1060g, erythritol 110.8300g, natural pomegranate flavor 6.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 31.5900g, sevenberry concentrate 21.0600g 1049 g of a fourth collagen composition was prepared.

Comparative Example 4_Preparation of the fifth collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 7.0235g, acerola concentrate 12.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 2.1060g, erythritol 120.8300g, natural pomegranate flavor 6.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 31.5900g, sevenberry concentrate 21.0600g 1058 g of the fifth collagen composition was prepared.

Comparative Example 5_Preparation of the sixth collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 7.0235g, acerola concentrate 12.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 2.1060g, erythritol 115.8300g, natural pomegranate flavor 6.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 21.5900g, sevenberry concentrate 16.0600g 1038 g of the sixth collagen composition was prepared.

Comparative Example 6_Preparation of the 7th collagen composition

Purified water 538.0198g, collagen 280.8351g, hyaluronic acid 4.6859g, red beet concentrate 7.0235g, acerola concentrate 12.6360g, taurine 17.5535g, essential amino acid 1.0530g, L-Arginine 0.3896g, Complex golden extract 1.1060g, erythritol 115.8300g, natural pomegranate flavor 5.3180g, enzyme-treated stevia 0.2106g, citric acid anhydrous 12.6360g, trisodium citrate 1.0530g, pomegranate concentrate 31.5900g, sevenberry concentrate 21.0600g 1053 g of a seventh collagen composition was prepared.

Experimental Example 5_ Collagen texture

The sensory test was comprehensively evaluated for sweetness, bitterness, sour taste, fishy taste, and aroma by 20 trained sensory test personnel, and the score ranges from 1 to 5 in the order from smallest to largest. The results are shown in Table 5 below.

symbol Example 1 5 Comparative Example 2 3 Comparative Example 3 3 Comparative Example 4 3 Comparative Example 5 3 Comparative Example 6 4

It was confirmed that the third collagen composition of Comparative Example 2 to the seventh collagen composition of Comparative Example 6 had inappropriate sweetness, sour taste, fishy taste, flavor, etc. became

In the above, even though it has been described that all the components constituting the embodiment of the present invention operate by being combined or combined into one, the present invention is not necessarily limited to this embodiment. That is, within the scope of the object of the present invention, all the components may operate by selectively combining one or more.

In addition, all terms including technical or scientific terms described above have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs, unless otherwise defined. Terms commonly used, such as those defined in the dictionary, should be interpreted as being consistent with the contextual meaning of the related art, and are not interpreted in an ideal or excessively formal meaning unless explicitly defined in the present invention.

And, the above description is merely illustrative of the technical idea of the present invention, and various modifications and variations will be possible without departing from the essential characteristics of the present invention by those skilled in the art to which the present invention pertains. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical spirit of the present invention, but to explain, and the scope of the technical spirit of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (5)

Purified water, collagen, hyaluronic acid, red beet concentrate, acerola concentrate, taurine, essential amino acids, L-arginine, complex golden extract, erythritol, natural pomegranate flavor, enzyme-treated stevia, citric acid anhydrous, citric acid A collagen composition comprising trisodium acid, pomegranate concentrate, and sevenberry concentrate.
The method according to claim 1,
50 to 52 parts by weight of purified water, 26 to 27 parts by weight of collagen, 0.4 to 0.5 parts by weight of hyaluronic acid, 0.6 to 0.7 parts by weight of red beet concentrate, 1.18 to 1.22 parts by weight of acerola concentrate, 1.6 to 1.7 parts by weight of taurine, 0.09 to 0.09 parts by weight of essential amino acids 0.11 parts by weight, L-arginine 0.035 to 0.039 parts by weight, complex golden extract 0.19 to 0.21 parts by weight, erythritol 10.8 to 11.2 parts by weight, natural pomegranate flavor 0.5 to 0.7 parts by weight, enzyme-treated stevia 0.198 to 0.022 parts by weight, citric anhydride (Citric acid) Acid Anhydrous) 1.1 to 1.3, 0.08 to 0.12 parts by weight of trisodium citrate, 2.9 to 3.1 parts by weight of pomegranate concentrate, and 1.9 to 2.1 parts by weight of sevenberry concentrate.
3. The method according to claim 2,
The collagen is
A collagen composition, characterized in that it is a collagen peptide having a size of 1 nm to 10 nm and a molecular weight of 250 Da to 650 Da.
3. The method according to claim 2,
The collagen is
A collagen composition having a size of 6 nm to 8 nm, an average molecular weight of 500 Da, and a collagen peptide.
3. The method according to claim 2,
The collagen is a collagen composition of any one or more of KTKS, GHK, AHK, and mixtures thereof.