KR20210077369A - Artificial feed composition of Protaetia brevitarsis Larva - Google Patents
Artificial feed composition of Protaetia brevitarsis Larva Download PDFInfo
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Abstract
Description
본 발명은 흰점박이꽃무지 유충 사육용 인공사료 조성물에 관한 것이다.The present invention relates to an artificial feed composition for rearing larvae of White-spotted flower radish larvae.
최근 곤충이 식량, 기능성 소재, 농업자재 등 미래의 유망한 자원으로 대두되면서 이의 지속적인 시장 성장이 예상되고 있다. 뿐만 아니라 쌍별귀뚜라미와 장수풍뎅이, 갈색거저리, 흰점박이꽃무지의 유충 등 4종이 일반식품원료로 등록됨에 따라 식용곤충에 대한 관심이 높아지고, 이와 더불어 곤충의 대량사육이 전국적으로 이루어지고 있다. 이에 생산 단계에서부터 안전하게 관리될 수 있도록 식용곤충 안전사육 및 관리의 표준화와 안전하고 고영양성분이 포함된 식용곤충 전용 먹이원의 개발에 관한 연구가 요구되고 있다.As insects have recently emerged as a promising resource for the future, such as food, functional materials, and agricultural materials, continuous market growth is expected. In addition, interest in edible insects is increasing as four species, such as twin-star cricket, long-lived beetle, brown mealworm, and larva of white-spotted flower beetle, are registered as general food raw materials, and in addition, mass breeding of insects is being carried out nationwide. Therefore, research on the standardization of safe breeding and management of edible insects and the development of a food source exclusively for edible insects containing safe and highly nutritious ingredients is required so that they can be safely managed from the production stage.
이중 흰점박이꽃무지(Protaetia brevitarsis)는 민간 및 동의보감 등의 전통 한방의서에서 "제조" 또는 "굼벵이" 라는 속명으로 불리어지고 있는 딱정벌레목, 풍뎅이과, 꽃무지아과에 속하며, 몸길이 17~22㎜, 폭 12~15㎜의 초식성 곤충으로서, 몸은 진한 구리빛이고 광택이 있으며 황백색 무늬가 흩어져 있다. Among them, Protaetia brevitarsis belongs to the coleoptera family , scarabaceae, and flower mussel family, which are called "manufacturing" or "slug" in traditional oriental medicine books such as folk and Donguibogam, and have a body length of 17~22㎜, It is a herbivorous insect with a width of 12-15 mm. The body is dark copper-colored, glossy, and yellowish-white patterns are scattered.
우리나라를 비롯하여 중국, 일본 및 시베리아 동부 지역에 서식하며, 4월에서 10월에 걸쳐 1 내지 2년에 1회 발생한다고 알려져 있다. 3령 유충은 평균 머리폭이 4.3~4.5㎜ 전후가 되며, 몸길이는 25~37㎜이며, 유충의 몸은 유백색이며 전체에 황색의 짧은 털들이 촘촘하게 나있다. 몸의 크기에 비해 머리 크기가 작고 다리가 발달되지 않아 이동 시 등을 이용하여 이동한다. 흰점박이꽃무지 외의 꽃무지류(Protaetia spp.)로는 잔꽃무지, 참꽃무지, 검정꽃무지, 홀쭉꽃무지, 풀색꽃무지, 넓적꽃무지, 범꽃무지, 참넓적꽃무지, 점박이꽃무지, 애초록꽃무지, 만주점박이꽃무지, 큰자색호랑꽃무지 등이 있으나 각각 별개의 곤충으로 인정된다.It lives in Korea, China, Japan, and eastern Siberia, and is known to occur once every 1-2 years from April to October. The average head width of 3rd instar larvae is around 4.3~4.5mm, and the body length is 25~37mm, and the body of the larva is milky white, with yellow short hairs on the whole. The head size is small compared to the body size and the legs are not developed, so it uses the back to move. Other than white-spotted flower radish (Protaetia spp.), small-flowered radish, black-flowered radish, black-flowered radish, green-flowered radish, broad-leaved radish, pan-flowered radish, spotted flower radish, primrose radish. There are Flower Muzi, Manchurian Spotted Flower Muzi, and Great Purple Tiger Flower Muzi, but each is recognized as a separate insect.
흰점박이꽃무지는 성충이 알을 낳아 부화해서 1령에서 3령(유충시기)을 거쳐 종령을 지나 번데기가 되며 번데기 후에 성충이 되는 완전탈바꿈을 하는 곤충에 속한다. 1령에서 3령까지의 발육기간은 약 4~5개월이 걸린다. 어린 유충의 성장 속도에 가장 중요한 영향을 미치는 것은 사육환경으로서 온도, 먹이, 습도 등의 요인들이 있다. 1령은 약 10일 경과 전후까지 성장을 하고 탈피를 하여 2령 유충이 된다. 몸이 점점 비대하며 커지고 약 20일 지나면 또 탈피를 한 후 이것이 3령 유충이 된다. 3령 유충은 약 3~4개월 동안 열심히 먹고 성장을 하여 종령이 되기 시작하면서 번데기로 될 준비를 한다. 종령의 구분은 3령의 유충 머리색으로 할 수 있으며 짙은 갈색으로 변화된 것이 종령이다.White-spotted flower radish belongs to insects that undergo complete metamorphosis, where adults lay eggs, hatch, pass through the first to third instars (larval stage), pass through the final instar, become pupae, and after pupa, become adults. The development period from 1st to 3rd instar takes about 4-5 months. The most important influence on the growth rate of young larvae is the breeding environment, and there are factors such as temperature, food, and humidity. 1st instar grows until about 10 days have elapsed and molts to become 2nd instar larva. The body gradually enlarges and enlarges, and after about 20 days, it molts again and this becomes a third instar larva. Third instar larvae eat hard for about 3 to 4 months, grow and begin to become larvae, preparing to pupate. The classification of the species can be done by the hair color of the larvae of 3 instars, and the ones that have changed to dark brown are the young ones.
흰점박이꽃무지는 3령의 성숙유충 상태로 월동하며, 성충은 주간에 활동하고 복숭아, 배 등의 성숙한 과일이나 옥수수, 상수리 나무 등의 즙액을 먹이로 하며, 항상 군집성이다. 또한, 이들의 유충은 퇴비나 건초더미 등의 유기물이 풍부한 부식성 토양속에서 서식한다.White-spotted flower radish overwinters as 3 instar mature larvae, and adults are active during the day and feed on mature fruits such as peaches and pears, or juices from corn and oak trees, and are always gregarious. In addition, their larvae live in humus soils rich in organic matter such as compost or haystacks.
흰점박이꽃무지는 오래전부터 간질환 등의 치료를 위한 한방 약재로서 이용되어 왔다. 또한, 최근에 유용한 생체 활성물질의 탐색 및 개발을 위한 곤충자원으로 크게 주목을 받고 있으며, 항생활성물질의 생산, 흰쥐(mouse)를 이용한 실험에서 알코올 과량섭취에 의해 손상된 간지질대사의 회복작용 등이 알려져 있으며 혈전용해성 효소에 대한 연구와 집쥐(rat)에서 사염화탄소의 투여에 의해 유도된 간독성에 대한 간보호효과를 나타내는 등 유용성이 확인된 바 있다.White-spotted flower radish has long been used as a herbal medicine for the treatment of liver diseases and the like. In addition, it has recently attracted great attention as an insect resource for the search and development of useful bioactive substances, and the production of antibiotics, the restoration of liver lipid metabolism damaged by excessive alcohol consumption in experiments using mice, etc. This is known, and its usefulness has been confirmed, such as a study on thrombolytic enzymes and a hepatoprotective effect on hepatotoxicity induced by the administration of carbon tetrachloride in rats.
그러나, 이러한 흰점박이꽃무지의 약용성에 관심이 집중되면서 많은 사람들이 흰점박이꽃무지를 대량으로 채집하려고 하는 반면, 자연산의 채집에는 어려움이 많다. 흰점박이꽃무지의 유충이 초가집 지붕 등에 주로 서식하지만 농촌가옥의 지붕개량 사업과 농약의 남용으로 인한 환경오염으로 인해 이들 흰점박이꽃무지가 멸종되어가고 있어, 현재는 농촌에서도 거의 찾아볼 수 없다.However, as attention has been focused on the medicinal properties of this white-spotted flower radish, many people are trying to collect a large amount of white-spotted flower radish, but there are many difficulties in collecting wild radish. Although the larvae of the white-spotted beetle mainly inhabit the roofs of thatched houses, they are becoming extinct due to environmental pollution caused by the roof improvement project of rural houses and the abuse of pesticides, and currently, they are hardly found in rural areas.
현재까지 흰점박이꽃무지의 생활사나 생태에 대하여는 우리나라의 경우 옥수수, 상추, 장미과의 과수류 5종, 귤나무류 2종, 닥나무류 2종, 잎갈나무류 2종, 참나무류 13종을 가해한다고 알려져 있으며(조용섭, 한국 식물병·해충·잡초명감, 한국식물보호학회, 633(1986)), 그 인공사육방법에 대해서는 발효된 볏짚 등을 이용한 방법(대한민국 특허 제 1998-069207호), 또는 톱밥 등을 이용한 방법(대한민국 특허 제 2000-0030598호, 제 2001-0499853호) 등이 알려져 있으나 발효기간이 오래 걸리거나 발효과정이 과학적으로 제시되지 못한 점이 있다. As for the life history and ecology of white-spotted flower igneous, it is known that in Korea, corn, lettuce, 5 types of rosaceae fruit trees, 2 types of tangerines, 2 types of mulberry trees, 2 types of oak trees, and 13 types of oak trees are harmful. (Jo Yong-seop, Korean Plant Diseases, Pests, and Weeds, Korean Society for the Protection of Plants, 633 (1986)), and for the artificial breeding method, a method using fermented rice straw, etc. (Korea Patent No. 1998-069207), or sawdust, etc. Methods using this (Korean Patent Nos. 2000-0030598, 2001-0499853) are known, but there are points that the fermentation takes a long time or the fermentation process is not scientifically suggested.
즉, 흰점박이꽃무지의 인공사육방법에 대한 관심이 점차 늘고는 있으나, 일부 민간수준에서의 사육방법이 알려져 있을 뿐, 흰점박이꽃무지 유충의 대량 생산에 대한 체계적인 연구가 부족한 실정이며, 급증하는 흰점박이꽃무지의 수요를 충족시키기에는 야외에서의 단순 채집 방법 및 자연상태에서의 증식으로 한계가 있어 유용자원으로서의 효율적인 활용이 어려운 형편이다.In other words, although interest in the artificial breeding method of white-spotted beetle is gradually increasing, some methods of breeding at the private level are known, and systematic research on the mass production of white-spotted flower larvae is lacking. In order to meet the demand for white-spotted daisy, it is difficult to efficiently utilize it as a useful resource because there is a limit to the simple method of collecting it outdoors and propagation in the natural state.
이에, 본 발명자들은, 식용 곤충으로 사육이 많이 되고 있는 흰점박이꽃무지 전용 먹이원을 확보하고 흰점박이꽃무지의 생육을 촉진하면서 경제적이고 안정적인 대량사육을 도모할 수 있는 곤충 사육용 인공사료 소재를 개발하기 위하여 예의 연구 노력하였다. 그 결과, 은행잎 분말을 흰점박이꽃무지 유충의 사료에 첨가했을 때, 건강하고 생육이 촉진된 흰점박이꽃무지 유충의 생산이 가능할 뿐만 아니라, 흰점박이꽃무지 유충의 성장 기간을 단축시켜 농가 생산력 향상 및 대량 생산에 유용함을 규명함으로써 본 발명을 완성하였다.Accordingly, the present inventors have provided an artificial feed material for insect breeding that can promote economical and stable mass breeding while securing a dedicated food source for white-spotted daisy, which is widely bred as an edible insect, and promoting the growth of white-spotted daisy. In order to develop it, intensive research efforts were made. As a result, when Ginkgo biloba powder is added to the feed of white spot caterpillars, it is possible not only to produce healthy and stimulated white spot caterpillars, but also to shorten the growth period of white spot caterpillars, thereby improving farm productivity. And the present invention was completed by finding that it is useful for mass production.
따라서, 본 발명의 일 목적은, 톱밥 및 영양원의 혼합 발효물; 및 은행잎 분말을 포함하는, 흰점박이꽃무지 (Protaetia brevitarsis) 사육용 인공사료 조성물을 제공하는 데 있다.Therefore, one object of the present invention, sawdust and a fermented mixture of nutrients; And to provide an artificial feed composition for breeding, comprising a ginkgo leaf powder, white spot flower radish ( Protaetia brevitarsis ).
또한, 본 발명의 다른 목적은, 톱밥 및 영양원의 혼합 발효물; 및 은행잎 분말을 혼합하여 혼합물을 제조하는 단계;를 포함하는, 흰점박이꽃무지 사육용 인공사료 제조방법을 제공하는 데 있다.In addition, another object of the present invention, the mixed fermented product of sawdust and nutrients; and preparing a mixture by mixing ginkgo leaf powder;
또한, 본 발명의 또 다른 목적은, 상기 사육용 인공사료 조성물을 흰점박이꽃무지에 급여하는 단계;를 포함하는 흰점박이꽃무지의 인공사육방법을 제공하는 데 있다.In addition, another object of the present invention is to provide an artificial breeding method of white spot flower radish, comprising the step of feeding the artificial feed composition for breeding to white spot flower radish.
또한, 본 발명의 또 다른 목적은, 흰점박이꽃무지 유충 발육 기간 단축용 인공사료 조성물을 제공하는 데 있다.In addition, it is another object of the present invention to provide an artificial feed composition for shortening the development period of larvae of white spot flower larvae.
또한, 본 발명의 또 다른 목적은, 흰점박이꽃무지 유충 발육 기간 단축용 인공사료 제조방법을 제공하는 데 있다.In addition, another object of the present invention is to provide a method for producing an artificial feed for shortening the development period of the larvae of the white spot flower larvae.
또한, 본 발명의 또 다른 목적은, 흰점박이꽃무지 유충의 발육 기간 단축 인공사육방법을 제공하는 데 있다.In addition, another object of the present invention is to provide an artificial breeding method for shortening the growth period of white spot larvae.
본 명세서에서 사용한 용어는 단지 설명을 목적으로 사용된 것으로, 한정하려는 의도로 해석되어서는 안된다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 명세서에서, "포함하다" 또는 "가지다" 등의 용어는 명세서 상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.The terms used herein are used for the purpose of description only, and should not be construed as limiting. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present specification, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 실시예가 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가지고 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 관련 기술의 문맥 상 가지는 의미와 일치하는 의미를 가지는 것으로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 일 양태에 따르면, 본 발명은 톱밥 및 영양원의 혼합 발효물; 및 은행잎 분말을 포함하는, 흰점박이꽃무지 유충 (Protaetia brevitarsis Larva) 사육용 인공사료 조성물을 제공한다.According to one aspect of the present invention, the present invention is a mixed fermented product of sawdust and a nutrient source; And it provides an artificial feed composition for breeding, containing a ginkgo leaf powder, larvae of white-spotted flower radish (Protaetia brevitarsis Larva).
본 발명의 인공사료 조성물의 특징은, 톱밥 및 영양원의 혼합 발효물에 은행잎 분말을 첨가하여 제조된 혼합 사료 조성물로서, 징코라이드(ginkgolide) A, 징코라이드 B, 징코라이드 C, 진놀(ginnol), 플라보놀(flavonol) 등의 성분을 함유하고 있어, 다양한 항균작용, 항진균 작용, 항암작용, 혈관계 조절작용 등 약리학적 작용으로 건강 증진에 도움이 되는 것으로 알려져 있는 은행잎 분말을 이용하여, 흰점박이꽃무지 유충에 대한 생육을 촉진시키고 세균 성장을 억제시킴으로써, 1일 내지 3령기의 흰점박이꽃무지 유충의 생존율 및 증체량을 향상시켰을 뿐만 아니라, 흰점박이꽃무지의 유충 기간(발육 기간)을 현저히 단축시켰다는 데 있다.Characteristics of the artificial feed composition of the present invention is that it is a mixed feed composition prepared by adding ginkgo leaf powder to a mixed fermented product of sawdust and a nutrient source, including ginger A, ginger B, ginger C, ginnol, Ginkgo biloba powder, which contains ingredients such as flavonol, is known to be helpful for health promotion through pharmacological actions such as various antibacterial, antifungal, anticancer, and vascular system control actions. By promoting the growth of larvae and inhibiting bacterial growth, the survival rate and weight gain of the larvae of 1 to 3 instar stage were improved, and the larval period (development period) of the larvae was significantly shortened. have.
본 발명의 인공 사료 조성물 내 은행잎 분말의 함량은, 본 발명의 조성물이 목적하는 효과, 즉, 흰점박이꽃무지 유충의 증체량 증가 및 유충기간 단축 효과가 달성되는 한, 특별히 한정되는 것은 아니나, 바람직하게는 은행잎 분말을 조성물 100중량부에 대하여, 3 내지 15중량부로 포함하며, 보다 바람직하게는 5 내지 10중량부로 포함하고, 가장 바람직하게는 10중량부로 포함한다. 상기 은행잎 분말의 함량이 조성물 100중량부에 대하여, 3중량부 미만인 경우에는 은행잎 분말을 첨가함으로써 얻어지는 생육 기간과 항균 작용이 미미하고, 15중량부를 초과하는 경우에는 흰점박이꽃무지의 생육 속도가 오히려 저하될 우려가 있다.The content of ginkgo leaf powder in the artificial feed composition of the present invention is not particularly limited, as long as the composition of the present invention achieves the desired effect, that is, the increase in the amount of weight gain and the effect of shortening the larval period of the larvae contains ginkgo leaf powder in an amount of 3 to 15 parts by weight based on 100 parts by weight of the composition, more preferably in an amount of 5 to 10 parts by weight, and most preferably in an amount of 10 parts by weight. When the content of the ginkgo leaf powder is less than 3 parts by weight based on 100 parts by weight of the composition, the growth period and antibacterial action obtained by adding the ginkgo leaf powder is insignificant, and when it exceeds 15 parts by weight, the growth rate of the white-spotted flower radish is rather There is a risk of deterioration.
본 발명의 인공 사료 조성물 내 톱밥 및 영양원의 혼합 발효물의 함량은, 본 발명의 조성물이 목적하는 효과, 즉, 흰점박이꽃무지 유충의 증체량 증가 및 유충기간 단축 효과가 달성되는 한, 이에 한정하지 않는다. The content of the mixed fermented product of sawdust and nutrient source in the artificial feed composition of the present invention is not limited thereto, as long as the composition of the present invention achieves the desired effect, that is, an increase in weight increase and a shortening of the larval period of white spot larvae. .
본 발명의 일 구현예에서, 톱밥 및 영양원을 혼합하여 발효처리한 혼합 발효물에, 은행잎 분말을 첨가하여 먹이원을 제조하였다.In one embodiment of the present invention, a food source was prepared by adding ginkgo leaf powder to a mixed fermented product fermented by mixing sawdust and a nutrient source.
상기 톱밥은 흰점박이꽃무지 유충의 서식처이면서 동시에 사료로 사용될 수 있다. 톱밥은 흰점박이꽃무지 유충이 서식할 수 있는 모든 종류의 톱밥을 사용할 수 있으나, 바람직하게는 참나무 톱밥일 수 있다.The sawdust can be used as a feed as well as a habitat for white spot larvae. The sawdust may be any type of sawdust in which the white spot larvae can be inhabited, but preferably oak sawdust.
상기 영양원은 흰점박이꽃무지 유충의 사료로 이용할 수 있는 영양 성분은 모두 포함할 수 있으며, 바람직하게는 밀기울, 당류 및 EM 발효액으로 이루어진 군으로부터 선택되는 하나 이상의 영양원일 수 있다.The nutrient source may include all of the nutrient components that can be used as feed for the larvae of the white spot larvae, and preferably may be one or more nutrient sources selected from the group consisting of bran, saccharides and EM fermentation broth.
상기 당류로는 과당, 설탕, 꿀, 올리고당으로 이루어진 것 중 선택되는 1종 이상 선택된 것을 사용할 수 있다.As the saccharide, one or more selected from among fructose, sugar, honey, and oligosaccharide may be used.
상기 EM 발효액은 EM 원액 또는 EM 활성액인 것을 특징으로 한다.The EM fermentation broth is characterized in that it is an EM stock solution or an EM activated solution.
본 발명의 일 구현예에서, 상기 톱밥 및 영양원의 혼합 발효물은, 혼합물 100중량부에 대하여 참나무 톱밥 90 내지 97.5중량부, 밀기울 1 내지 5중량부, 당류 1 내지 5중량부, EM 발효액 0.5 내지 2중량부를 혼합한 후 발효시켜서 제조할 수 있다. In one embodiment of the present invention, the mixed fermented product of sawdust and nutrient source is 90 to 97.5 parts by weight of oak sawdust, 1 to 5 parts by weight of bran, 1 to 5 parts by weight of sugar, 0.5 to 5 parts by weight of EM fermentation broth with respect to 100 parts by weight of the mixture. It can be prepared by mixing 2 parts by weight and then fermenting.
본 발명의 일 구현예에서, 상기 조성물은 상기 톱밥 및 영양원의 혼합 발효물을 조성물 100중량부에 대하여, 80 내지 97중량부로 포함하는 것이 바람직하며, 85 내지 95중량부로 포함하는 것이 보다 바람직하나, 이에 제한되지 않는다. In one embodiment of the present invention, the composition preferably contains 80 to 97 parts by weight, more preferably 85 to 95 parts by weight, based on 100 parts by weight of the composition of the mixed fermented product of sawdust and nutrient sources, It is not limited thereto.
본 발명의 인공사료 조성물은 추가적으로 수크로스(sucrose), 말토스(maltose), 락토스(lactose), 락툴로스(lactulose), 트레할로스(trehalose), 셀로비오스(cellobiose) 및 키토비오스(chitobiose)로 이루어진 군에서 선택되는 1종 이상의 당원을 포함할 수 있다.The artificial feed composition of the present invention additionally comprises sucrose, maltose, lactose, lactulose, trehalose, cellobiose and chitobiose. It may include one or more sugar members selected from
상기 수크로스는 프룩토스 한 분자 및 글루코스 한 분자가 글리코시드 결합으로 연결된 이당류로, 인공사료 내에서 주요 탄소원이다. 인공사료에 포함된 수크로스는 곤충의 모든 세포, 특히, 지방체, 비행근육, 소화관과 체벽의 표피세포에 존재하는 글리코겐을 합성에 이용된다.The sucrose is a disaccharide in which one molecule of fructose and one molecule of glucose are linked by a glycosidic bond, and is a major carbon source in artificial feed. Sucrose contained in artificial feed is used for synthesizing glycogen present in all cells of insects, especially fat bodies, flying muscles, and epidermal cells of the digestive tract and body wall.
본 발명의 바람직한 구현예에서, 상기 조성물은 조성물 100중량부에 대해, 은행잎 5 내지 10중량부 및 톱밥 및 영양원의 혼합 발효물 90 내지 95중량부를 포함하는 것이 바람직하나, 이에 제한되지 않는다.In a preferred embodiment of the present invention, the composition preferably includes 5 to 10 parts by weight of ginkgo leaves and 90 to 95 parts by weight of a mixed fermented product of sawdust and a nutrient source based on 100 parts by weight of the composition, but is not limited thereto.
상기 인공사료 조성물은 비타민 복합체 또는 복합무기염류을 더 포함할 수 있으나, 이에 제한되지 않는다. 또한 본 발명의 인공사료 조성물은 당업자가 예상할 수 있는 범위 내의 다른 기타 영양성분을 추가로 포함할 수 있다.The artificial feed composition may further include a vitamin complex or complex inorganic salts, but is not limited thereto. In addition, the artificial feed composition of the present invention may further include other nutrients within the range that those skilled in the art can expect.
본 발명에 따른 인공사료 조성물은 흰점박이꽃무지 유충의 인공사육에 최적화된 것으로서, 이를 식이한 흰점박이꽃무지 유충의 생존율 및 무게가 현저히 증가하고, 유충 기간이 단축되는 것을 확인하였다. It was confirmed that the artificial feed composition according to the present invention was optimized for the artificial breeding of white spot larvae, and the survival rate and weight of the larvae of genus oleifera were significantly increased, and the larval period was shortened.
따라서, 본 발명에 따른 인공사료 조성물은 흰점박이꽃무지 유충의 생육을 촉진시키는 흰점박이꽃무지 유충 사육용 인공사료 조성물일 뿐만 아니라, 유충 기간을 단축시키는 흰점박이꽃무지 유충의 사육 기간 단축용 인공사료 조성물을 제공함으로써, 흰점박이꽃무지 유충 및 성충 사육의 효율을 높일 수 있다. Therefore, the artificial feed composition according to the present invention is not only an artificial feed composition for breeding larvae of larvae of larvae of larvae, but also an artificial feed composition for shortening the period of larvae of larvae of larvae. By providing the feed composition, it is possible to increase the efficiency of breeding larvae and adults.
본 발명의 다른 양태에 따르면, 본 발명은 혼합물 100중량부에 대해 톱밥 및 영양원의 혼합 발효물 80 내지 97중량부 및 은행잎 분말 3 내지 15중량부를 혼합하여 혼합물을 제조하는 단계;를 포함하는, 흰점박이꽃무지 유충 사육용 인공사료 제조방법을 제공한다.According to another aspect of the present invention, the present invention comprises the steps of preparing a mixture by mixing 80 to 97 parts by weight of a mixed fermented product of sawdust and a nutrient source and 3 to 15 parts by weight of ginkgo leaf powder with respect to 100 parts by weight of the mixture; Provided is a method for manufacturing artificial feed for breeding spotted flower larvae.
본 발명의 일 구현예에서, 상기 단계에 앞서 톱밥 및 영양원을 혼합한 후 발효시키는 단계를 더 포함할 수 있으나, 이에 제한되지 않는다. In one embodiment of the present invention, it may further include a step of fermenting after mixing sawdust and a nutrient source prior to the step, but is not limited thereto.
본 발명의 일 구현예에서, 흰점박이꽃무지 유충 사육용 인공사료 제조방법에 따라 제조된 인공사료를 급이하고 개체중을 확인한 결과, 생장촉진 효과가 있음을 알 수 있었으며, 곤충에 병원성을 나타내는 주요 세균 중 하나인 세라티아 마르세센스 (Serratia marcescens)와 같은 세균에 대해서도 강력한 항균 효과를 나타내며 흰점박이꽃무지 유충에 안전하다는 것을 알 수 있었다.In one embodiment of the present invention, as a result of feeding the artificial feed prepared according to the method for producing artificial feed for rearing white-spotted larvae and checking the weight of the individuals, it was found that there was a growth promoting effect, indicating pathogenicity to insects. It was found that it showed a strong antibacterial effect against bacteria such as Serratia marcescens , which is one of the main bacteria, and was safe against white spot larvae.
본 발명의 또 다른 양태에 따르면, 본 발명은 상기 흰점박이꽃무지 유충 사육용 인공사료 조성물을 흰점박이꽃무지 유충에 급여(급이)하는 단계;를 포함하는 흰점박이꽃무지 유충의 인공사육방법을 제공한다.According to another aspect of the present invention, the present invention provides an artificial breeding method of white spot flower radish larvae comprising the step of feeding (feeding) the artificial feed composition for rearing white spot flower radish larvae. provides
본 발명의 인공사육방법은 상술한 흰점박이꽃무지 유충 사육용 인공사료 조성물을 이용하므로, 중복된 내용은 본 명세서의 과도한 복잡성을 피하기 위하여 그 기재를 생략한다.Since the artificial breeding method of the present invention uses the above-described artificial feed composition for breeding larvae of the white-spotted flower larvae, redundant descriptions thereof are omitted in order to avoid excessive complexity of the present specification.
본 발명에 따른 흰점박이꽃무지 유충 사육을 위한 인공사료 조성물은 흰점박이꽃무지 유충의 생존율 및 체중을 현저히 증가시킬 수 있으며, 유충 기간을 탁월하게 단축시키므로 대량 사육할 수 있다는 장점이 있다. 이는 본 발명에 따른 인공사료 조성물은 흰점박이꽃무지를 연중 대량 사육할 수 있음을 의미하는 바, 곤충 사육 분야, 나아가 제약 및 식품 분야에서 다양하게 활용될 수 있다.The artificial feed composition for breeding larvae of the larvae of the present invention can significantly increase the survival rate and weight of larvae of the larvae, and it has the advantage of being able to breed in large quantities because the larval period is shortened significantly. This means that the artificial feed composition according to the present invention can be mass-bred throughout the year, and it can be used in various fields including insect breeding, as well as pharmaceutical and food fields.
도 1은 먹이원별 흰점박이꽃무지 유층의 생존율 결과를 나타낸 도이다.
도 2는 먹이원 급여에 따른 흰점박이꽃무지 유층의 증체량(상단 그래프) 및 유충 크기(하단 이미지) 비교 결과를 나타낸 도이다.
도 3은 은행잎의 세균 성장 억제 효과를 나타낸 도이다.
도 4는 본 발명의 인공사료 조성물에 포함된 은행잎 분말 첨가에 따른 세균 감염 유충의 성장률을 확인한 결과를 나타낸 도이다.
도 5는 본 발명의 인공사료 조성물에 포함된 은행잎 분말 첨가에 따른 세균 감염 유충의 증체율을 확인한 결과를 나타낸 도이다.1 is a diagram showing the results of the survival rate of the oily layer of white-spotted flower radish by food source.
2 is a diagram showing the results of comparison of the weight gain (upper graph) and larvae size (lower image) of the oily layer of white-spotted flower radish according to the feeding of the food source.
3 is a diagram showing the effect of inhibiting bacterial growth of ginkgo leaves.
4 is a view showing the results of confirming the growth rate of the bacterial infection larvae according to the addition of ginkgo leaf powder contained in the artificial feed composition of the present invention.
5 is a view showing the results of confirming the growth rate of bacterial infection larvae according to the addition of ginkgo leaf powder contained in the artificial feed composition of the present invention.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.
실시예Example
실시예 1. 흰점박이꽃무지 유충 사육을 위한 인공사료 제조Example 1. Manufacture of artificial feed for breeding larvae of white-spotted flower radish larvae
본 발명자들은 흰점박이꽃무지 유충의 사육을 위하여, 은행잎 분말을 첨가한 먹이원(인공사료)을 제조하였다. 상기 은행잎 분말 첨가 먹이원의 조성은 하기 표 1에 나타내었다.The present inventors prepared a food source (artificial feed) to which ginkgo leaf powder was added for the rearing of white-spotted flower radish larvae. The composition of the ginkgo leaf powder-added food source is shown in Table 1 below.
이러한 은행잎은 징코라이드(ginkgolide) A, 징코라이드 B, 징코라이드 C, 진놀(ginnol), 플라보놀(flavonol) 등의 성분을 함유하고 있어 말초혈관 장애, 노인성 치매 등을 치료 예방하는 데 효과를 가지고 있는 것으로 알려져 있으며, 육계나 돼지와 같은 가축의 사료 첨가제로도 이용되고 있다.These ginkgo leaves contain ingredients such as ginger A, ginger B, ginger C, ginnol, and flavonol, which are effective in treating and preventing peripheral vascular disorders and senile dementia. It is known that there is, and it is also used as a feed additive for livestock such as broilers and pigs.
상기 기본 사료는, 발효 톱밥을 이용하였으며, 상기 톱밥 혼합 발효물은 혼합물 100중량부에 대하여 참나무 톱밥 90중량부, 밀기울 5중량부, 당류 3중량부, EM 발효액 2중량부의 혼합물에 물을 첨가하여 함수율을 65% 내외로 조절하여 골고루 섞어 마대자루에 담은 후 비닐로 덮어 상온으로 조절된 실내에서 60일 발효하여 제작하였다. The basic feed, fermented sawdust was used, and the sawdust mixed fermented product was water added to a mixture of 90 parts by weight of oak sawdust, 5 parts by weight of bran, 3 parts by weight of sugar, and 2 parts by weight of EM fermentation broth with respect to 100 parts by weight of the mixture. The moisture content was adjusted to around 65%, mixed evenly, put in a sackcloth, covered with plastic, and fermented for 60 days in a room controlled at room temperature.
상기 표 1과 같은 조성으로 혼합하여 실내 발효장에서 30일 이상 발효시킨 후 흰점박이꽃무지 유충에 급여하였다. It was mixed with the composition shown in Table 1 and fermented for at least 30 days in an indoor fermenting plant, and then fed to the larvae of White Spotted flower radish.
첨가물을 넣지 않은 발효 톱밥을 대조구로 사용하였고, 처리구별 25개체씩 3회 반복하였다.Fermented sawdust without additives was used as a control, and 25 specimens for each treatment group were repeated 3 times.
실시예 2. 인공사료 내 은행잎 분말 첨가에 따른 흰점박이꽃무지 유충의 생존율 비교Example 2. Comparison of Survival Rates of White Spotted Flowering Radish Larvae according to the Addition of Ginkgo Leaf Powder in Artificial Feed
상기 실시예 1에서 제조한 인공사료를 식이한 흰점박이꽃무지 유충의 생존율을 비교하였다. The survival rate of the white spot flower larvae fed the artificial feed prepared in Example 1 was compared.
그 결과, 도 1에 나타낸 바와 같이, 5% GL 그룹 및 10% GL 그룹은 흰점박이꽃무지 유충의 생존율이 대조구보다 높은 것을 확인하였다(p=0.2815). As a result, as shown in FIG. 1 , it was confirmed that the survival rate of white spot larvae in the 5% GL group and the 10% GL group was higher than that of the control group ( p = 0.2815).
통계적 유의성은 ANOVA test를 실시하여 Tukey's multiple comparisons test를 통해 p<0.05 일 때 유의성이 있는 것으로 간주하였다. 통계 분석 결과, 값이 높은 순서대로 소문자 a부터 사용하여 통계적 유의성이 없는 실험군끼리 같은 문자로 표기하였다.Statistical significance was considered to be significant when p<0.05 through Tukey's multiple comparisons test by performing ANOVA test. As a result of statistical analysis, lowercase a was used in ascending order of value, and the same letter was used for experimental groups without statistical significance.
실시예 3. 인공사료 내 은행잎 분말 첨가에 따른 흰점박이꽃무지 유충의 증체량 비교Example 3. Comparison of weight gain of white spot larvae according to the addition of ginkgo leaf powder in artificial feed
상기 실시예 1에서 인공사료를 식이한 흰점박이꽃무지 유충의 증체량을 비교하였다. In Example 1, the amount of weight gain of the larvae of the white-spotted flower larvae fed the artificial feed was compared.
그 결과, 도 2에 나타낸 바와 같이, 흰점박이꽃무지 유충(3령)을 대상으로 각각 1%, 5% 및 10% 은행잎 분말 첨가 먹이원을 급이했을 때, 대조구 및 1% 은행잎 분말 첨가 먹이원과 비교하여 증체율이 높게 나타났으며, 특히, 5% 및 10% 은행잎 분말 첨가 먹이원 급여 후 유충 증체율이 급격히 증가하였고, 대조구 대비 약 2주나 빠른 성장률을 나타냈다.As a result, as shown in FIG. 2 , when feeding the larvae with 1%, 5%, and 10% ginkgo leaf powder, respectively, the control group and 1% ginkgo leaf powder added food to the larvae (3rd instar). The growth rate was higher than that of the original, and, in particular, the growth rate of the larvae increased rapidly after feeding with the 5% and 10% ginkgo leaf powder added, and the growth rate was about 2 weeks faster than the control group.
또한, 먹이원 급이 20일 후, 5% 및 10% 은행잎 분말 첨가 먹이원 급이 그룹의 유충 무게는 각각 3.04 g과 3.12 g으로 대조구에 비해 46~50% 이상 현저하게 증가하였다(5% 은행잎; p=4.20 x 10-6, 10% 은행잎; p=2.32 x 10-6). In addition, after 20 days of feeding, the weight of the larvae of the group fed with 5% and 10% ginkgo leaf powder was 3.04 g and 3.12 g, respectively, which significantly increased by 46-50% or more compared to the control group (5% ginkgo leaf). ; p =4.20 x 10 -6 , 10% ginkgo leaves; p =2.32 x 10 -6 ).
통계적 유의성은 상기의 통계 분석 방법을 이용하여 유의성 분석을 실시하였다.Statistical significance was analyzed using the above statistical analysis method.
실시예 4. 인공사료 내 은행잎 분말 첨가에 따른 흰점박이꽃무지 유충의 영기별 성장기간 비교Example 4. Comparison of growth period by reiki period of white-spotted flower larvae according to the addition of ginkgo leaf powder in artificial feed
상기 실시예 1에서 제조된 인공사료를 식이한 흰점박이꽃무지 유충의 영기별 성장 기간을 비교하였다. The growth period by age of the larvae of the white spot flower larvae fed the artificial feed prepared in Example 1 was compared.
그 결과, 하기 표 2에 나타낸 바와 같이, 은행잎 분말 첨가 먹이원 급이 시 흰점박이꽃무지 유충의 2령과 3령 기간은 대조구과 대비했을 때 절반 이하로 단축되었다(2령; p=1.15ⅹ10-15, 3령; p=7.63ⅹ10-19). 특히, 10% 은행잎 분말 첨가 먹이원 급이 시, 총 유충기간은 약 46일로 대조구 대비 57% 이상 단축되는 것으로 나타났다.As a result, as shown in Table 2 below, the 2nd and 3rd instar larvae of Ginkgo biloba larvae were shortened to less than half when fed with ginkgo leaf powder-added food source compared to the control group (2nd instar; p = 1.15×10 - 15 , 3rd instar; p =7.63×10 -19 ). In particular, when fed with 10% ginkgo leaf powder, the total larval period was about 46 days, which was reduced by more than 57% compared to the control group.
상기 결과는 본 발명의 은행잎 분말 첨가 인공사료가 흰점박이꽃무지 유충의 성장 기간을 현저히 단축시킨다는 것을 의미한다.The above result means that the artificial feed added with ginkgo leaf powder of the present invention significantly shortens the growth period of white spot larvae.
통계적 유의성은 상기 통계 분석 방법을 이용하여 유의성 분석을 실시하였다.Statistical significance was analyzed using the above statistical analysis method.
실시예 5. 인공사료 내 은행잎 분말 첨가에 따른 흰점박이꽃무지 유충의 항균 활성 비교Example 5. Comparison of antibacterial activity of white spot larvae according to the addition of ginkgo leaf powder in artificial feed
상기 실시예 1에서 제조된 인공사료를 식이한 흰점박이꽃무지 유충의 항균 활성을 비교하였다. The antibacterial activity of the larvae of white-spotted flower radish fed the artificial feed prepared in Example 1 was compared.
먼저, 곤충에 병원성을 나타내는 주요 세균 중 하나인 세라티아 마르세센스 (Serratia marcescens) 배양 배지에 1% 은행잎 분말 첨가(0.25Х107 cfu/ml)했을 때 대조구(1.57Х108 cfu/ml)에 비해 세균의 콜로니가 60 배 이상 감소(p=0.007939)된 것을 확인하였다(도 3).First, when 1% ginkgo leaf powder was added (0.25Х107 cfu/ml) to the culture medium of Serratia marcescens , one of the major bacteria showing pathogenicity to insects, compared with the control (1.57Х10 8 cfu/ml), It was confirmed that colonies were reduced by more than 60 times ( p = 0.007939) (FIG. 3).
통계적 유의성은 상기 통계 분석 방법을 이용하여 유의성 분석을 실시하였다.Statistical significance was analyzed using the above statistical analysis method.
또한, 은행잎의 in vitro 항균 활성을 확인하기 위해, 세라티아 마르세센스 (Serratia marcescens) 배지 현탁액(5Х103 cfu)을 경피주사하여 상기 세균에 감염된 흰점박이꽃무지 유충에게 은행잎 분말 첨가 먹이원을 4 주 급여한 후 생존율 및 증체율을 관찰하였다.In addition, in order to confirm the in vitro antibacterial activity of ginkgo leaves, the Serratia marcescens medium suspension (5Х10 3 cfu) was injected percutaneously to the larvae infected with the bacteria, and a food source with the addition of ginkgo leaf powder was given for 4 weeks. After feeding, the survival rate and weight gain were observed.
그 결과, 도 4에 나타낸 바와 같이, 세라티아 마르세센스에 감염된 흰점박이꽃무지 유충에게 5% 및 10% 은행잎 분말 첨가 먹이원을 급여한 경우, 상기 감염 유충의 생존율은 은행잎 분말-무첨가 대조구((+)Control) 먹이원을 급여한 감염 유충보다 높게 나타났다 (5% 은행잎; p=0.02198, 10% 은행잎; p=0.0529).As a result, as shown in FIG. 4 , when Serratia marcescens-infected larvae of white-spotted flowers were fed with a food source supplemented with 5% and 10% ginkgo leaf powder, the survival rate of the infected larvae was obtained from the ginkgo leaf powder-free control (( +) Control) was higher than that of the infected larvae fed the food source (5% ginkgo leaf; p =0.02198, 10% ginkgo leaf; p =0.0529).
통계적 유의성은 상기 통계 분석 방법을 이용하여 유의성 분석을 실시하였다.Statistical significance was analyzed using the above statistical analysis method.
또한, 도 5에 나타낸 바와 같이, 세라티아 마르세센스에 감염된 흰점박이꽃무지 유충에게 5% 및 10% 은행잎 분말 첨가 먹이원을 급여한 경우, 상기 감염 유충의 증체율은 각각 28.4%와 23.6%로, 은행잎 분말-무첨가 먹이원 급여한 감염 유충의 증체율(-0.2%)보다 높았다 (5% 은행잎; p=7.96 x 10-12, 10% 은행잎; p=2.69 x 10-10).In addition, as shown in FIG. 5, when Serratia marcescen-infected larvae of white spot flower were fed with 5% and 10% ginkgo leaf powder-added food sources, the growth rates of the infected larvae were 28.4% and 23.6%, respectively, The growth rate (-0.2%) of the infected larvae fed with ginkgo leaf powder-free food source was higher (5% ginkgo leaf; p =7.96 x 10 -12 , 10% ginkgo leaf; p =2.69 x 10 -10 ).
통계적 유의성은 상기의 통계 분석 방법을 이용하여 유의성 분석을 실시하였다.Statistical significance was analyzed using the above statistical analysis method.
상기 결과는 본 발명의 은행잎 분말 첨가 인공사료가 흰점박이꽃무지 유충의 항균 활성을 탁월하게 증가시킨다는 것을 의미한다.The above result means that the artificial feed added with ginkgo leaf powder of the present invention excellently increases the antibacterial activity of white spot larvae.
종합적으로, 본 발명자들은 흰점박이꽃무지 유충의 생산성을 개선시킬 수 있는 은행잎 분말을 5% 및 10%로 첨가한 먹이원인 인공사료를 제조하였고, 상기 은행잎 분말을 5% 및 10%로 첨가한 먹이원을 급여하였을 때, 흰점박이꽃무지 유충의 무게는 증체하였고 유충 기간은 단축된 효과를 나타냈다. 또한, 세균 감염된 유충에 은행잎 첨가 먹이원을 급여했을 때 현저하게 세균의 성장을 억제하여 유충의 생존율 및 유충의 무게가 증가하였다.Overall, the present inventors prepared an artificial feed, which is a food source, in which ginkgo leaf powder was added at 5% and 10%, which could improve the productivity of white spot larvae, and the feed added the ginkgo leaf powder at 5% and 10%. When the raw material was fed, the weight of the larvae of the larvae increased and the duration of the larvae was shortened. In addition, when a food source added with ginkgo leaves was fed to bacterially infected larvae, the growth rate of the larvae and the weight of the larva were increased by significantly inhibiting the growth of bacteria.
즉, 식용곤충으로 많이 사육되고 있는 흰점박이꽃무지 유충의 먹이원에 활용한 결과, 생육 촉진 효과를 확인하였을 뿐만 아니라, 세균 감염된 유충에서도 현저하게 세균의 성장을 억제한 바, 다양한 약리 작용으로 건강 증진에 도움이 될 수 있는 건강한 흰점박이꽃무지 유충의 생산이 가능할 것으로 예상된다.In other words, as a result of using it as a food source for white-spotted larva larvae that are widely bred as edible insects, it not only confirmed the effect of promoting growth, but also significantly inhibited the growth of bacteria in larvae infected with bacteria. It is expected that it will be possible to produce healthy white spot larvae that can be helpful in promotion.
따라서, 은행잎 첨가가 유충의 빠른 성장과 건강한 유충의 생산을 유도함으로써 곤충사육농가에서 흰점박이꽃무지 전용 먹이원으로 활용하여 사육농가의 노동력을 절감할 수 있을 뿐만 아니라 경쟁력을 향상시킬 수 있을 것이다.Therefore, by inducing the rapid growth of larvae and the production of healthy larvae, the addition of ginkgo leaves can be used as a food source exclusively for white-spotted flowers in insect breeding farms, thereby reducing labor force of breeding farms and improving competitiveness.
이상, 본 발명내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적인 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의해 정의된다고 할 것이다. Above, a specific part of the present invention has been described in detail, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.
Claims (10)
mixed fermented products of sawdust and nutrient sources; And, comprising a ginkgo leaf powder, white spot flower larvae ( Protaetia brevitarsis Larva) artificial feed composition for breeding.
상기 은행잎 분말은 조성물 100중량부에 대하여 3 내지 15중량부로 포함되는 것을 특징으로 하는,
흰점박이꽃무지 유충 사육용 인공사료 조성물.
According to claim 1,
The ginkgo leaf powder is characterized in that it is included in an amount of 3 to 15 parts by weight based on 100 parts by weight of the composition,
An artificial feed composition for rearing white-spotted flower larvae.
상기 영양원은 밀기울, 당류 및 EM 발효액으로 이루어진 군으로부터 선택되는 하나 이상의 영양원인 것을 특징으로 하는,
흰점박이꽃무지 유충 사육용 인공사료 조성물.
According to claim 1,
The nutrient source is characterized in that at least one nutrient source selected from the group consisting of wheat bran, saccharides and EM fermentation broth,
An artificial feed composition for rearing white-spotted flower larvae.
mixed fermented products of sawdust and nutrient sources; And preparing a mixture by mixing the ginkgo leaf powder; Containing, Artificial feed manufacturing method for breeding white-spotted flower larvae (Protaetia brevitarsis Larva).
상기 은행잎 분말은 혼합물 100중량부에 대하여 3 내지 15중량부로 포함되는 것을 특징으로 하는,
흰점박이꽃무지 유충 사육용 인공사료 제조방법.
5. The method of claim 4,
The ginkgo leaf powder is characterized in that it is included in an amount of 3 to 15 parts by weight based on 100 parts by weight of the mixture,
A method of manufacturing artificial feed for breeding white-spotted flower larvae.
상기 영양원은 밀기울, 당류 및 EM 발효액으로 이루어진 군으로부터 선택되는 하나 이상의 영양원인 것을 특징으로 하는,
흰점박이꽃무지 유충 사육용 인공사료 제조방법.
5. The method of claim 4,
The nutrient source is characterized in that at least one nutrient source selected from the group consisting of wheat bran, saccharides and EM fermentation broth,
A method of manufacturing artificial feed for breeding white-spotted flower larvae.
An artificial breeding method of white spot flower radish larvae, comprising the step of feeding the white spot flower radish larvae with the artificial feed composition for rearing white spot flower radish larvae according to any one of claims 1 to 3.
mixed fermented products of sawdust and nutrient sources; And, comprising a ginkgo leaf powder, white spot flower larvae ( Protaetia brevitarsis Larva) artificial feed composition for shortening the growth period.
mixed fermented products of sawdust and nutrient sources; And preparing a mixture by mixing the ginkgo leaf powder; Containing, Artificial feed manufacturing method for shortening the growth period of white-spotted flower larvae (Protaetia brevitarsis Larva).
10. An artificial breeding method for shortening the growth period of white spot flower larvae, comprising the step of applying the artificial feed composition for shortening the development period of the white spot flower larva according to claim 8 to the larvae of the white spot flower larva.
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