KR102230056B1 - Breeding method of larva of Protaetia brevitarsis - Google Patents

Abstract

The present invention relates to a method of breeding white-spotted radish larva, and more specifically, inoculating a mushroom mycelium in a sawdust medium to cultivate the mycelium to form a mushroom fruiting body (S10); Culturing the fruiting bodies of the mushroom mycelium in a bottle medium and then pulverizing the mushroom mycelium using a crusher to harvest the mushroom mycelium powder containing sawdust (S20); Fermenting the sawdust medium in a fermentor to prepare a fermented product (S30); Injecting the fermented product into a breeding box and then germinating barley from the fermented product to grow sprout barley (S40); And it relates to a method of breeding white-spotted radish larvae comprising the step (S50) of breeding white-spotted radish larva after putting the mushroom mycelium powder containing sawdust into the breeding box.

Description

Breeding method of larva of Protaetia brevitarsis}

The present invention relates to a method of breeding white-spotted radish larva, and more particularly, to a method of breeding white-spotted radish larva using a fermented sawdust medium.

The white-spotted flower is a herbivorous insect with a body length of 17 to 22 mm and a width of 12 to 15 mm, and the body is dark copper-colored, glossy, and yellow-white patterns are scattered. It lives in Korea, China, Japan, and eastern Siberia, and is known to occur once every 1-2 years from April to October. The larvae of the white-spotted flower radish are classified into 1-3 instars according to their size, and the average head width of the 3rd instar larvae is around 43~45㎜ and the body length is 25~37㎜. The body of the larva of this period is milky white, and there are densely yellow short hairs on the whole. Compared to the size of the body, the head is small and the legs are not developed, so when moving, it is moved using a light. Protaetia spp other than white-spotted flowers include fine flowers, true flowers, black flowers, white flowers, grass-colored flowers, broad-brimmed flowers, pan-flowered flowers, true broad-colored flowers, spotted flowers, and first green flowers. There are ignorance, Manchurian spotted flower ignorance, and large purple tiger flower ignorance.

The white-spotted flower radish winters in the state of 3 instar mature larvae, and adults are active during the day and feed on mature fruits such as peaches and pears, or juice from corn and oak trees, and are always clustered. In addition, their larvae live in corrosive soils rich in organic matter such as compost and haystacks.

The white-spotted flower is one of the insects that are completely transformed into adult insects after the adult insects lay eggs and hatch, pass through the 1st to 3rd instars, and then become the pupae after the pupae. The growth period from 1 to 3 years of age takes about 4 to 5 months. The most important influence on the growth rate of young larvae is the breeding environment, which includes factors such as temperature, food, and humidity. The first instar grows until about 10 days elapsed and molts to become a second instar larva. The body becomes enlarged and enlarged, and after about 20 days, it becomes a 3rd instar larva after another molting. The 3rd instar larvae eat and grow hard for about 3-4 months and prepare to become a pupa as they begin to become a species. The species can be classified by the color of the larvae of 3 instars, and the ones that have changed to golden color are the species.

Recently, as attention has been focused on the medicinal properties of white-spotted flowers, many people try to collect large amounts of white-spotted flowers, but there are many difficulties in collecting wild flowers. As a prior art for feed for breeding white spotted radish, sulfur fermented sawdust for slug culture including oyster mushroom mycelium extract produced from cottonseed, rice bran, beet pulp and sulfur-containing oak sawdust medium, and a method for manufacturing the sulfur sawdust (Publication Patent No. 2018-0117837) is disclosed, and as a prior art for a method of artificial breeding of white spotted radish, a method using fermented rice straw, etc. (Korea Patent Registration No. 10-0228541), using oak sawdust. Although the method (Korea Patent Registration No. 10-0398862) is known, it has not yet disclosed a method for breeding white-spotted radish larva, which has an excellent effect of promoting the growth of white-spotted radish larva.

Republic of Korea Patent Publication No. 2018-0117837 (2018.10.30)

Accordingly, it is an object of the present invention to provide a method for breeding white spotted radish larvae having an excellent effect of promoting the growth of white-spotted radish larva.

The method of rearing white-spotted flower radish larvae according to an embodiment of the present invention for achieving the above object comprises the steps of inoculating a mushroom mycelium in a sawdust medium to cultivate the mycelium to form a mushroom fruiting body (S10); Culturing the fruiting bodies of the mushroom mycelium in a bottle medium and then pulverizing the mushroom mycelium using a crusher to harvest the mushroom mycelium powder containing sawdust (S20); Fermenting the sawdust medium in a fermentor to prepare a fermented product (S30); Injecting the fermented product into a breeding box and then germinating barley from the fermented product to grow sprout barley (S40); And after putting the mushroom mycelium powder containing the sawdust into the breeding box may include the step (S50) of breeding the white spotted radish larva.

In addition, in the method of breeding white-spotted flower radish larvae according to the present invention, the sawdust medium is characterized in that it comprises silver aspen sawdust, dermis and rice bran.

In addition, in the method of breeding white spotted radish larvae according to the present invention, the step S30 includes Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus in the sawdust medium. reuteri) is added to the mixture to prepare a fermented fermented product.

According to the present invention, the method of breeding white spotted radish larva according to the present invention is excellent in the effect of promoting the growth of white spotted radish larva.

In addition, it is possible to improve the breeding process and increase the breeding productivity by providing the feed provided for the breeding of the white spotted radish larva in a simpler and more efficient process.

1 is a flow chart for a method of breeding white spotted flower radish larva according to the present invention.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be described in detail in the detailed description. However, this is not intended to limit the present invention to a specific embodiment, it should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, when it is determined that a detailed description of a related known technology may obscure the subject matter of the present invention, a detailed description thereof will be omitted.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In the present application, terms such as "comprise" or "have" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements or numbers, steps, actions, components, parts, or combinations thereof does not preclude in advance.

Terms such as first and second may be used to describe various elements, but the elements should not be limited by the terms. The above terms are used only for the purpose of distinguishing one component from another component.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. It is apparent to those skilled in the art that the present invention can be embodied in other specific forms without departing from the spirit and essential features of the present invention.

1 is a flow chart for a method of breeding white spotted flower radish larva according to the present invention.

Referring to Figure 1, the method of breeding white-spotted flower radish larvae according to a preferred embodiment of the present invention comprises the steps of forming a mushroom fruiting body (S10); Harvesting the mushroom mycelium powder containing sawdust (S20); Manufacturing a fermented product (S30); Germinating barley from the fermented product to grow sprout barley (S40); And it may include the step (S50) of rearing the larvae of white spotted flowers.

The step of forming the mushroom fruiting bodies according to step S10 is to inoculate the mushroom mycelium in a sawdust medium to cultivate the mycelium to form a mushroom fruiting body.

The mushroom may be any one of oyster mushroom, reishi mushroom and cordyceps mushroom, preferably oyster mushroom, but is not limited thereto.

Oyster mushroom is a mushroom belonging to the pine mushroom family, and in Chinese characters it is called Cheonhwasim·man. In autumn, it grows in large numbers of dry trunks of broad-leaved trees such as oak, alder, cottonwood, willow, etc., fallen tree trunks, and cut stumps, and is distributed not only in Korea but also in China, Japan, Europe, North America, and Siberia. . Fruiting bodies occur in October-December and March-April, and the caps are grayish white or light grayish brown, forming a semicircle or fan shape. Artificial cultivation is done by cultivation of logs or bottle cultivation of sawdust. When seeding is inoculated in spring on logs, fruiting bodies are formed in autumn.

To inoculate the oyster mushroom mycelium, first prepare the oyster mushroom spawn. To this end, oyster mushroom progenitors were transplanted into PDA, MEA medium, etc. in a sterile phase, and then hyphae cultured for 10 to 15 days in an incubator at a temperature of 25 to 28°C, and preserved at -20°C and used.

The sawdust medium is prepared by mixing 65% by weight of silver sawdust, 23% by weight of dermis, and 12% by weight of rice bran, which are the main materials, followed by mixing and kneading water.

Here, the tree used as the main material of the medium is aspen (scientific name: Populus tomentiglandulosa), and the silver aspen is a natural hybrid formed between aspen (P. davidiana) and Eunbaekyang (P. alba). As a biological classification, plant family (Plantae), pizza plant (Angiospermae) dicotyledonae (Dicotyledoneae) willow tree (Salicales) willow tree family (Salicaceae). It is beautiful and can be used as a tree planting, guidance planting, and buffer planting. In the present invention, silver ash is used as sawdust. Logs are harvested from around November when the sap stops moving to February of the following year, and is naturally dried in the shade after logging. When the moisture content is 35 to 45%, the solid wood is cut to about 12 to 15 cm in diameter and 20 cm in length, and is prepared by crushing into 40 mesh grains with a sawdust maker.

The dermis (Citrus Unshiu Peel) refers to the dried peel of the mature fruit of Citrus unshiu Markovich. As a scented tendon placebo, it is used for gastritis and indigestion, and as an antitussive, antitussive, and expectorant. In the present invention, the dermis may be purchased commercially, or may be harvested or cultivated in nature.

Rice bran is called rice bran when it is milled from brown rice to white rice. Brown rice is the rice hull that is peeled off the outer husk of rice by threshing the rice, and white rice (white rice) is the rice hull and the inner husk (rice bran) after grinding the brown rice.

A substance called "GABA", which lowers high blood pressure in rice snow, has the function of preventing adult diseases by reducing triglycerides in the blood and enhancing liver function. Rice bran improves blood circulation and activates the natural healing power of humans. It boosts immunity and turns a weak acidic constitution into a healthy constitution. Rice bran is rich in vitamins such as hemicellulose, dietary fiber, inositol, choline, niacin (B3), tocopherol (E), thiamine (B1), and pantothenic acid (B5).

After adjusting the moisture content of the medium to 65% to 70%, put about 1 kg each in a heat-resistant polypropylene bag and close the cap to perform 7 hours at normal pressure sterilization (100°C) and 90 minutes for high pressure sterilization (121°C). . Cool the autoclaved medium to about 15℃ before seed inoculation.

Oyster mushroom spawns cultured in a sterile room or sterile bed are inoculated into the sterilized and cooled sawdust medium in an inoculation room maintained at 15°C. Inoculation method is to add about 100g of seed per 2kg of sawdust medium. At this time, the work of opening and closing the heat-resistant plastic bag should be performed as soon as possible with the shortest exposure time to prevent contamination by various germs. The sawdust medium after inoculation is cultured for 25 to 30 days in a culture chamber maintained at a temperature of 20 to 25°C. At the beginning of cultivation, the temperature is maintained at about 20 to 22°C for 7 days, and then culture is performed at about 25°C after the oyster mushroom spawns are settled on the sawdust medium.

The cultivation of fruiting bodies in the sawdust medium in which the cultivated oyster mushroom spawns are established is carried out by transferring the bottle medium in which the mycelium cultured at 25° C. for 30 days is cultured to a cultivation room at 18° C. and 95% relative humidity to form fruiting bodies do.

In the step of harvesting the mushroom mycelium powder containing sawdust according to step S20, the fruiting bodies of the mushroom mycelium are cultivated in a bottle medium and then pulverized using a crusher to harvest the mushroom mycelium powder containing sawdust.

The fruiting bodies of oyster mushroom mycelium are cultured at 25℃ for 30 days in a solid bottle medium, dried to a moisture content of 4-5% at 60℃, and then pulverized to a particle size of 100 mesh using a crusher. Finally, a medium containing sawdust. To obtain a mycelium powder containing oyster mushroom.

The step of preparing the fermented product according to step S30 is to ferment the sawdust medium in a fermenter to prepare a fermented product.

As in the case of step S10, the sawdust medium is prepared by mixing 65% by weight of silver ash sawdust, 23% by weight of dermis, and 12% by weight of rice bran, and then mixing and kneading water, as in the case of step S10. At this time, preferably, when the fermented product of the present invention is manufactured using the waste sawdust medium discarded after cultivation of oyster mushrooms, there is an advantage of reducing production cost.

A fermented product can be prepared by fermenting a mixture of Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus reuteri in the sawdust medium.

Here, the Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus reuteri are mixed at a weight ratio of 1:1:1.

The fermentation microorganism may be introduced in the form of an EM (Effective Microorganisms) stock solution. EM (Effective Microorganism) is an abbreviation of a useful microbial group. It is a complex microbial preparation in which 80 groups of 10 kinds of microorganisms that are beneficial to humans are combined and cultured among many microorganisms existing in nature. Protobacteria constituting EM bacteria are generally yeast, lactic acid bacteria, yeast, photosynthetic bacteria, Actinomyces, and mold fungi, which have been used by humans for a long time in food fermentation, etc. It is a group of microorganisms that lead in the direction of revitalizing nature by microbial coexistence, producing antioxidant or antioxidant substances, and suppressing spoilage.

The EM (Effective-Microorgarnisms) fermentation broth used in the present invention can be either an EM stock solution or an EM active solution distributed on the market. The EM active solution is mixed with 0.1 to 2 parts by weight of saccharides (at least one selected from fructose, sugar, honey, and oligosaccharides) to 100 parts by weight of water, and 5 to 20 parts by weight of the EM stock solution in water in which the saccharides are mixed After mixing, it can be made by incubating in an anaerobic state for 1 to 5 days at a temperature of 29 to 35°C. More preferably, 100 parts by weight of water, 1 part by weight of sugar, and 20 parts by weight of EM stock solution are mixed at a temperature of 32°C. It can be prepared by incubating for 3 days.

More preferably, the EM (Effective-Microorgarnisms) fermentation broth used in the present invention is Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus reuteri in the EM stock solution. By mixing, each strain may be included in an amount of ^{3.0x10 6 cfu/g or more.} In this case, the nutrient content, fermentation properties, and microbial phase of the mushroom waste medium are further improved, and the effect of preventing the occurrence of mold in the feed composition for white spotted radish larvae can be further improved by further improving the harmful bacteria inhibiting effect. .

The step of growing sprout barley by germinating barley from the fermentation according to step S40 is to grow sprout barley by adding the fermented product to a breeding box and then germinating barley from the fermented product.

The step of rearing the white-spotted radish larvae according to step S50 is to breed the white-spotted radish larva after putting the mushroom mycelium powder containing the sawdust into the breeding box. The white-spotted radish larvae are bred while ingesting the mushroom mycelium powder containing the sawdust and sprout barley grown from the fermented product.

Hereinafter, the present invention will be described in more detail through examples, but the following examples are for illustrative purposes only, and are not intended to limit the scope of the present invention.

<Example>

Oyster mushroom waste medium, which is a raw material of silver ash sawdust, was pulverized to have an average diameter of 10 mm using a pulverizer, and prepared as the main raw material for food. A sawdust medium was prepared by adding 23 parts by weight of dermis and 12 parts by weight of rice bran to 100 parts by weight of the main feed material, followed by stirring.

Oyster mushroom mycelium was inoculated in the sawdust medium to harvest the fruiting bodies of oyster mushrooms, and then the fruiting bodies of the mushroom mycelium were cultivated in a bottle medium, and then pulverized using a crusher to harvest the mushroom mycelium powder containing sawdust.

Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus in a sawdust medium prepared by the same process of oyster mushroom waste medium, which is raw material from silver ash sawdust, and sawdust medium containing dermis and rice bran. An EM fermentation broth containing Lactobacillus reuteri (Lactobacillus reuteri) in a commercially-purchased EM stock solution, each containing 3.0x10 ⁶ cfu/g or more, is directly added to the blender in a volume of 10 L, corresponding to 10% by weight of the total solid, and the fermentation microorganism A fermented product was prepared by stirring the added blend at a rate of 6 hr/day for 14 days in an anaerobic blend fermenter (SH-sp 220V) having an initial temperature of 35° C. and maintaining the moisture content at 40%.

Thereafter, the fermented product was added to the breeding box, and barley was germinated from the fermented product to grow sprout barley, and the mushroom mycelium powder was added to the breeding box, and then the white spotted radish larva was reared.

<Comparative Example>

For the comparative experiment, sawdust, cotton thread, beet pulp, and sulfur were prepared from oak. Those prepared in this way were subjected to the conditions of Table 1 below, but the remaining steps were carried out in the same manner as in the above example. At this time, in Comparative Examples 1 to 4 and Comparative Examples 6 to 7, Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus reuteri were mixed with commercially available EM stock solutions. Thus ^{, an EM fermentation broth containing 3.0x10 6} cfu/g or more, respectively, was used in Comparative Examples 8 to 13, an EM fermentation broth obtained by mixing only 1 to 2 strains of the above strains. Comparative Example 14 is ^{an EM fermentation broth containing 3.0x10 6} cfu/g or more by mixing Lactobacillus plantarum in a commercially-purchased EM stock solution, and Comparative Example 15 is Bacillus subtilis in a commercially-purchased EM stock solution. ^{EM fermentation broth containing 3.0x10 6} cfu/g or more was used by mixing Bacillus subtilis.

division Silver Sashimi Sawdust (g) Oak sawdust (g) Dermis (g) Rice bran (g) Cotton thread, beet pulp, sulfur (g) Strains containing EM fermentation broth Comparative Example 1 100 EM fermentation broth containing a+b+c Comparative Example 2 100 EM fermentation broth containing a+b+c Comparative Example 3 100 EM fermentation broth containing a+b+c Comparative Example 4 100 EM fermentation broth containing a+b+c Comparative Example 5 65 12 Cotton thread 12, beet pearl 11,
Sulfur 2 Comparative Example 6 65 35 EM fermentation broth containing a+b+c Comparative Example 7 65 35 EM fermentation broth containing a+b+c Comparative Example 8 65 23 12 EM fermentation broth containing a Comparative Example 9 65 23 12 EM fermentation broth containing b Comparative Example 10 65 23 12 EM fermentation broth containing c Comparative Example 11 65 23 12 EM fermentation broth containing a+b Comparative Example 12 65 23 12 EM fermentation broth containing b+c Comparative Example 13 65 23 12 EM fermentation broth containing a+c Comparative Example 14 65 23 12 EM fermentation broth containing Lactobacillus plantarum Comparative Example 15 65 23 12 EM fermentation broth containing Bacillus subtilis

* a: Streptococcus thermophilus

* b: Bifidobacterium longum

* c: Lactobacillus reuteri

The strains used in the Examples and Comparative Examples of the present invention are those sold by biotechnology-related companies, or microbial deposit institutions at home and abroad (gene bank of the Institute of Bioscience and Biotechnology, Korea Microbial Conservation Center, Agricultural Genetic Resource Center, ATCC (American Type). Culture CollectionAsociacin)).

<Experimental Example>

Eight adult white-spotted flowers (4 females and 4 males) were secured per experimental group, and the hatching larvae obtained by mating and spawning these adults (1 instar larvae) were used as test worms. The white-spotted radish grown under the conditions of Examples and Comparative Examples 1 to 13 of the present invention was reared until it became an adult to the hatching larva, and the larvae weight, fable rate, and adult appearance of each white-spotted radish are shown in Table 2 below. It shows a comparison of times.

division Weight of larvae at the end of 3 instars (mg) Allegory rate (%) Time of appearance of adult insects (days) Example 2524.4 98.8 101.9 Comparative Example 1 1934.8 90.2 155.5 Comparative Example 2 1987.5 91.2 153.9 Comparative Example 3 1826.2 92.8 163.0 Comparative Example 4 1855.4 91.4 157.9 Comparative Example 5 2287.6 92.4 165.8 Comparative Example 6 2318.4 93.6 135.1 Comparative Example 7 2311.8 93.2 139.8 Comparative Example 8 2353.7 93.0 133.9 Comparative Example 9 2308.3 93.8 128.9 Comparative Example 10 2327.7 93.9 129.5 Comparative Example 11 2388.8 93.0 130.2 Comparative Example 12 2350.0 93.2 136.6 Comparative Example 13 2347.4 93.3 140.0 Comparative Example 14 2124.3 92.5 155.6 Comparative Example 15 2145.5 92.6 154.8

Comparing the Examples of the present invention and Comparative Examples 1 to 15, in the case of the Examples of the present invention, the weight of the larvae at the end of the 3rd instar of the white-spotted flower radish was significantly increased, the larvae's allegory rate was also improved, and the time of appearance of the adult worms was also Was shortened.

On the other hand, the above detailed description should not be construed as restrictive in all respects and should be considered as illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention.

Claims (3)

Inoculating the mushroom mycelium on a sawdust medium containing silver ash sawdust, dermis and rice bran to cultivate the mycelium to form a mushroom fruiting body (S10);
Culturing the fruiting bodies of the mushroom mycelia in a bottle medium and then pulverizing the mushroom mycelium using a crusher to harvest the mushroom mycelium powder containing sawdust (S20);
The step of producing a fermented product by fermenting in a fermenter by introducing the microbial complex bacteria into a sawdust medium containing silver ash wood sawdust, dermis, and rice bran, which are the same as the sawdust medium of step S10 (S30);
Injecting the fermented product into a breeding box and then germinating barley from the fermented product to grow sprout barley (S40); And
Including; the step of breeding white spotted radish larvae after putting the mushroom mycelium powder containing the sawdust into the breeding box (S50); Including,
In the step S30, the microbial complex bacterium is a mixture of Streptococcus thermophilus, Bifidobacterium longum, and Lactobacillus reuteri. Way.
The method of claim 1,
In step S10,
The sawdust medium comprises 100 parts by weight of silver ash sawdust, 23 parts by weight of dermis, and 12 parts by weight of rice bran larvae breeding method.
The method of claim 1,
The mushroom is a white spotted flower radish larva breeding method, characterized in that the oyster mushroom.

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