KR20200123458A - 돌연변이를 도입하는 방법 - Google Patents

돌연변이를 도입하는 방법 Download PDF

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KR20200123458A
KR20200123458A KR1020207027106A KR20207027106A KR20200123458A KR 20200123458 A KR20200123458 A KR 20200123458A KR 1020207027106 A KR1020207027106 A KR 1020207027106A KR 20207027106 A KR20207027106 A KR 20207027106A KR 20200123458 A KR20200123458 A KR 20200123458A
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nucleic acid
acid molecule
target nucleic
mutation
sequence
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Korean (ko)
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리 쥐 모나한
조이스 투
캐서린 엠 버크
마이클 이멜포트
아론 이 달링
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롱가스 테크놀로지즈 피티와이 엘티디
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Publication of KR20200123458A publication Critical patent/KR20200123458A/ko
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020207027106A 2018-02-20 2019-02-19 돌연변이를 도입하는 방법 Pending KR20200123458A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1802744.1 2018-02-20
GBGB1802744.1A GB201802744D0 (en) 2018-02-20 2018-02-20 Method
PCT/GB2019/050443 WO2019162657A1 (en) 2018-02-20 2019-02-19 Method for introducing mutations

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Publication Number Publication Date
KR20200123458A true KR20200123458A (ko) 2020-10-29

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US (3) US11421238B2 (https=)
EP (3) EP4008792B1 (https=)
JP (2) JP7413283B2 (https=)
KR (1) KR20200123458A (https=)
CN (1) CN112088219B (https=)
AU (1) AU2019223315B2 (https=)
CA (1) CA3091770A1 (https=)
DK (1) DK3673084T3 (https=)
ES (2) ES2889548T3 (https=)
GB (1) GB201802744D0 (https=)
SG (1) SG11202007924TA (https=)
WO (1) WO2019162657A1 (https=)

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* Cited by examiner, † Cited by third party
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GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
CN119301686A (zh) 2023-03-10 2025-01-10 因美纳有限公司 基于突变序列读取确定核酸序列的系统和方法
WO2025255494A1 (en) 2024-06-07 2025-12-11 Illumina, Inc. Identifying the origin of amplified rna molecules
WO2026072259A1 (en) 2024-09-30 2026-04-02 Illumina, Inc. Methods, systems, and kits for sequencing nucleic acids with deamination and mutagenesis

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GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
EP3870718B1 (en) 2018-10-26 2023-11-29 The Board of Trustees of the Leland Stanford Junior University Methods and uses of introducing mutations into genetic material for genome assembly

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US20220348940A1 (en) 2022-11-03
EP4008792B1 (en) 2025-08-06
US11421238B2 (en) 2022-08-23
WO2019162657A1 (en) 2019-08-29
CN112088219B (zh) 2025-07-18
US20250137000A1 (en) 2025-05-01
CN112088219A (zh) 2020-12-15
ES3043588T3 (en) 2025-11-25
EP4640847A2 (en) 2025-10-29
EP3673084B1 (en) 2021-08-11
ES2889548T3 (es) 2022-01-12
EP4008792C0 (en) 2025-08-06
EP4640847A3 (en) 2025-12-10
AU2019223315B2 (en) 2025-07-10
DK3673084T3 (da) 2021-11-08
JP2024038164A (ja) 2024-03-19
CA3091770A1 (en) 2019-08-29
SG11202007924TA (en) 2020-09-29
AU2019223315A1 (en) 2020-10-01
JP2021514205A (ja) 2021-06-10
GB201802744D0 (en) 2018-04-04
EP3673084A1 (en) 2020-07-01
JP7413283B2 (ja) 2024-01-15
JP7717145B2 (ja) 2025-08-01
EP4008792A1 (en) 2022-06-08
US12203076B2 (en) 2025-01-21
US20210010008A1 (en) 2021-01-14

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