SG11202007924TA - Method for introducing mutations - Google Patents

Method for introducing mutations

Info

Publication number
SG11202007924TA
SG11202007924TA SG11202007924TA SG11202007924TA SG11202007924TA SG 11202007924T A SG11202007924T A SG 11202007924TA SG 11202007924T A SG11202007924T A SG 11202007924TA SG 11202007924T A SG11202007924T A SG 11202007924TA SG 11202007924T A SG11202007924T A SG 11202007924TA
Authority
SG
Singapore
Prior art keywords
introducing mutations
mutations
introducing
Prior art date
Application number
SG11202007924TA
Inventor
Leigh G Monahan
Joyce To
Catherine M Burke
Michael Imelfort
Aaron E Darling
Original Assignee
Longas Tech Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Longas Tech Pty Ltd filed Critical Longas Tech Pty Ltd
Publication of SG11202007924TA publication Critical patent/SG11202007924TA/en

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11202007924TA 2018-02-20 2019-02-19 Method for introducing mutations SG11202007924TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1802744.1A GB201802744D0 (en) 2018-02-20 2018-02-20 Method
PCT/GB2019/050443 WO2019162657A1 (en) 2018-02-20 2019-02-19 Method for introducing mutations

Publications (1)

Publication Number Publication Date
SG11202007924TA true SG11202007924TA (en) 2020-09-29

Family

ID=61783821

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202007924TA SG11202007924TA (en) 2018-02-20 2019-02-19 Method for introducing mutations

Country Status (12)

Country Link
US (2) US11421238B2 (en)
EP (2) EP4008792A1 (en)
JP (2) JP7413283B2 (en)
KR (1) KR20200123458A (en)
CN (1) CN112088219A (en)
AU (1) AU2019223315A1 (en)
CA (1) CA3091770A1 (en)
DK (1) DK3673084T3 (en)
ES (1) ES2889548T3 (en)
GB (1) GB201802744D0 (en)
SG (1) SG11202007924TA (en)
WO (1) WO2019162657A1 (en)

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
WO2002079502A1 (en) 2001-03-28 2002-10-10 The University Of Queensland A method for nucleic acid sequence analysis
EP2292767B1 (en) 2004-06-04 2013-11-20 Takara Bio, Inc. Polypepdides having DNA polymerase activity
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
JP5279339B2 (en) 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
EP2539464B1 (en) 2010-02-23 2016-11-16 Illumina, Inc. Amplification methods to minimise sequence specific bias
AU2011253984A1 (en) * 2010-12-07 2012-06-28 Biobalance Llc Method For Identifying E. Coli M-17
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
AU2015210705B2 (en) * 2014-01-31 2020-11-05 Integrated Dna Technologies, Inc. Improved methods for processing DNA substrates
CN104232761A (en) 2014-08-27 2014-12-24 武汉凯吉盈科技有限公司 Gene complete segment quick sequencing method
AU2015330685B2 (en) 2014-10-10 2022-02-17 Cold Spring Harbor Laboratory Random nucleotide mutation for nucleotide template counting and assembly
US10487340B2 (en) 2015-10-09 2019-11-26 Yunnan Academy Of Tobacco Agricultural Sciences Tobacco mosaic virus resistant N'au gene and cloning methods and applications thereof
CN107760771A (en) 2017-11-16 2018-03-06 杭州迪安医学检验中心有限公司 A kind of breast cancer susceptibility gene BRCA1 and BRCA2 full genomes capture primer, kit and its method
CN107955806B (en) 2017-12-15 2020-03-31 中国科学院深海科学与工程研究所 Preparation method and application of superoxide dismutase Cu, Zn SOD (superoxide dismutase) from deep-sea cucumber
AU2019366472A1 (en) 2018-10-26 2021-05-27 The Board Of Trustees Of The Leland Stanford Junior University Methods and uses of introducing mutations into genetic material for genome assembly

Also Published As

Publication number Publication date
JP7413283B2 (en) 2024-01-15
CN112088219A (en) 2020-12-15
EP4008792A1 (en) 2022-06-08
KR20200123458A (en) 2020-10-29
GB201802744D0 (en) 2018-04-04
US20210010008A1 (en) 2021-01-14
JP2021514205A (en) 2021-06-10
US20220348940A1 (en) 2022-11-03
EP3673084A1 (en) 2020-07-01
DK3673084T3 (en) 2021-11-08
EP3673084B1 (en) 2021-08-11
ES2889548T3 (en) 2022-01-12
CA3091770A1 (en) 2019-08-29
AU2019223315A1 (en) 2020-10-01
JP2024038164A (en) 2024-03-19
US11421238B2 (en) 2022-08-23
WO2019162657A1 (en) 2019-08-29

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