AU2019223315B2 - Method for introducing mutations - Google Patents

Method for introducing mutations Download PDF

Info

Publication number
AU2019223315B2
AU2019223315B2 AU2019223315A AU2019223315A AU2019223315B2 AU 2019223315 B2 AU2019223315 B2 AU 2019223315B2 AU 2019223315 A AU2019223315 A AU 2019223315A AU 2019223315 A AU2019223315 A AU 2019223315A AU 2019223315 B2 AU2019223315 B2 AU 2019223315B2
Authority
AU
Australia
Prior art keywords
glu
lys
arg
leu
ile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019223315A
Other languages
English (en)
Other versions
AU2019223315A1 (en
Inventor
Catherine M. BURKE
Aaron E. DARLING
Michael IMELFORT
Leigh G. MONAHAN
Joyce To
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Singapore Pte Ltd
Original Assignee
Illumina Singapore Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Singapore Pte Ltd filed Critical Illumina Singapore Pte Ltd
Publication of AU2019223315A1 publication Critical patent/AU2019223315A1/en
Assigned to ILLUMINA SINGAPORE PTE. LTD. reassignment ILLUMINA SINGAPORE PTE. LTD. Request for Assignment Assignors: Longas Technologies Pty Ltd
Application granted granted Critical
Publication of AU2019223315B2 publication Critical patent/AU2019223315B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2019223315A 2018-02-20 2019-02-19 Method for introducing mutations Active AU2019223315B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1802744.1 2018-02-20
GBGB1802744.1A GB201802744D0 (en) 2018-02-20 2018-02-20 Method
PCT/GB2019/050443 WO2019162657A1 (en) 2018-02-20 2019-02-19 Method for introducing mutations

Publications (2)

Publication Number Publication Date
AU2019223315A1 AU2019223315A1 (en) 2020-10-01
AU2019223315B2 true AU2019223315B2 (en) 2025-07-10

Family

ID=61783821

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019223315A Active AU2019223315B2 (en) 2018-02-20 2019-02-19 Method for introducing mutations

Country Status (12)

Country Link
US (3) US11421238B2 (https=)
EP (3) EP4008792B1 (https=)
JP (2) JP7413283B2 (https=)
KR (1) KR20200123458A (https=)
CN (1) CN112088219B (https=)
AU (1) AU2019223315B2 (https=)
CA (1) CA3091770A1 (https=)
DK (1) DK3673084T3 (https=)
ES (2) ES2889548T3 (https=)
GB (1) GB201802744D0 (https=)
SG (1) SG11202007924TA (https=)
WO (1) WO2019162657A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
CN119301686A (zh) 2023-03-10 2025-01-10 因美纳有限公司 基于突变序列读取确定核酸序列的系统和方法
WO2025255494A1 (en) 2024-06-07 2025-12-11 Illumina, Inc. Identifying the origin of amplified rna molecules
WO2026072259A1 (en) 2024-09-30 2026-04-02 Illumina, Inc. Methods, systems, and kits for sequencing nucleic acids with deamination and mutagenesis

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5565340A (en) * 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
NZ306767A (en) * 1995-04-11 2000-03-27 Univ Johns Hopkins Method of identifying molecular interactions employing counterselection and at least two hybrid molecules or two hybrid systems
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
WO2001088174A1 (en) * 2000-05-17 2001-11-22 The Trustees Of Boston University Novel compositions and methods for carrying out multiple pcr reactions on a single sample
WO2002079502A1 (en) 2001-03-28 2002-10-10 The University Of Queensland A method for nucleic acid sequence analysis
JP2005501527A (ja) 2001-05-30 2005-01-20 ストラタジーン カリフォルニア 無作為な核酸変異誘発のための組成物及び方法
US20030215914A1 (en) * 2001-12-10 2003-11-20 Erwin Houtzager Structure for presenting desired peptide sequences
WO2004038007A2 (en) * 2002-10-25 2004-05-06 Stratagene Dna polymerases with reduced base analog detection activity
CA2568998A1 (en) 2004-06-04 2005-12-15 Takara Bio Inc. Polypeptides having dna polymerase activity
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
JP5279339B2 (ja) 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物
WO2011106368A2 (en) * 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
AU2011253984A1 (en) * 2010-12-07 2012-06-28 Biobalance Llc Method For Identifying E. Coli M-17
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
CN103555685A (zh) * 2013-04-26 2014-02-05 江南大学 增强β-环糊精葡萄糖基转移酶产β-环糊精能力的突变方法
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
CN104232761A (zh) 2014-08-27 2014-12-24 武汉凯吉盈科技有限公司 基因全片段快速测序方法
US11008606B2 (en) 2014-10-10 2021-05-18 Cold Spring Harbor Laboratory Random nucleotide mutation for nucleotide template counting and assembly
US10487340B2 (en) 2015-10-09 2019-11-26 Yunnan Academy Of Tobacco Agricultural Sciences Tobacco mosaic virus resistant N'au gene and cloning methods and applications thereof
US11821028B2 (en) * 2016-07-12 2023-11-21 QIAGEN Sciences, LLP Single end duplex DNA sequencing
CN109312391B (zh) * 2016-07-18 2022-06-03 豪夫迈·罗氏有限公司 生成用于单分子测序的单链环状dna文库的方法
CN107760771A (zh) 2017-11-16 2018-03-06 杭州迪安医学检验中心有限公司 一种乳腺癌易感基因brca1和brca2全基因捕获引物、试剂盒及其方法
CN107955806B (zh) 2017-12-15 2020-03-31 中国科学院深海科学与工程研究所 一种深渊海参来源的超氧化物歧化酶Cu,Zn SOD的制备方法及其应用
GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
EP3870718B1 (en) 2018-10-26 2023-11-29 The Board of Trustees of the Leland Stanford Junior University Methods and uses of introducing mutations into genetic material for genome assembly

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUNG-SUK CHOI ET AL: "The use of modified and non-natural nucleotides provide unique insights into pro-mutagenic replication catalyzed by polymerase eta", NUCLEIC ACIDS RESEARCH, vol. 44, no. 3, 18 February 2016 pages 1022 - 1035 *
MASAYASU KUWAHARA ET AL: "Study on Suitability of KOD DNA Polymerase for Enzymatic Production of Artificial Nucleic Acids Using Base/Sugar Modified Nucleoside Triphosphates", MOLECULES, vol. 15, no. 11, 12 November 2010 pages 8229 - 8240 *

Also Published As

Publication number Publication date
US20220348940A1 (en) 2022-11-03
EP4008792B1 (en) 2025-08-06
US11421238B2 (en) 2022-08-23
WO2019162657A1 (en) 2019-08-29
CN112088219B (zh) 2025-07-18
US20250137000A1 (en) 2025-05-01
CN112088219A (zh) 2020-12-15
ES3043588T3 (en) 2025-11-25
EP4640847A2 (en) 2025-10-29
EP3673084B1 (en) 2021-08-11
ES2889548T3 (es) 2022-01-12
EP4008792C0 (en) 2025-08-06
EP4640847A3 (en) 2025-12-10
DK3673084T3 (da) 2021-11-08
JP2024038164A (ja) 2024-03-19
CA3091770A1 (en) 2019-08-29
SG11202007924TA (en) 2020-09-29
AU2019223315A1 (en) 2020-10-01
JP2021514205A (ja) 2021-06-10
GB201802744D0 (en) 2018-04-04
KR20200123458A (ko) 2020-10-29
EP3673084A1 (en) 2020-07-01
JP7413283B2 (ja) 2024-01-15
JP7717145B2 (ja) 2025-08-01
EP4008792A1 (en) 2022-06-08
US12203076B2 (en) 2025-01-21
US20210010008A1 (en) 2021-01-14

Similar Documents

Publication Publication Date Title
US20230099446A1 (en) Fast pcr for str genotyping
US12203076B2 (en) Method for introducing mutations
EP2971080B1 (en) Methods for amplification and sequencing using thermostable tthprimpol
JP3745774B2 (ja) 末端反復増幅方法
EP2173872B1 (en) Copy dna and sense rna
CA2948951C (en) Synthesis of double-stranded nucleic acids
US20050118578A1 (en) Amplified nucleic acids and immobilized products thereof
KR20220113522A (ko) 주형-비의존성 핵산을 합성하는 방법 및 키트
JP2021514205A5 (https=)
WO2022144003A1 (zh) 一种用于高通量靶向测序的多重pcr文库构建方法
HK40075402B (en) Method for introducing mutations
HK40075402A (en) Method for introducing mutations
HK40031786A (en) Method for introducing mutations
HK40031786B (en) Method for introducing mutations
US20080014616A1 (en) Methods of introducing targeted diversity into nucleic acid molecules

Legal Events

Date Code Title Description
PC1 Assignment before grant (sect. 113)

Owner name: ILLUMINA SINGAPORE PTE. LTD.

Free format text: FORMER APPLICANT(S): LONGAS TECHNOLOGIES PTY LTD

FGA Letters patent sealed or granted (standard patent)