KR20200115552A - 별개의 용융 분석을 위한 방법 및 조성물 - Google Patents

별개의 용융 분석을 위한 방법 및 조성물 Download PDF

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KR20200115552A
KR20200115552A KR1020207024174A KR20207024174A KR20200115552A KR 20200115552 A KR20200115552 A KR 20200115552A KR 1020207024174 A KR1020207024174 A KR 1020207024174A KR 20207024174 A KR20207024174 A KR 20207024174A KR 20200115552 A KR20200115552 A KR 20200115552A
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nucleic acid
signal
target
probe
predetermined
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Korean (ko)
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더글러스 휘트먼
제니퍼 베르니에
윌리엄 왕
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루미넥스 코포레이션
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020207024174A 2018-01-22 2019-01-22 별개의 용융 분석을 위한 방법 및 조성물 Ceased KR20200115552A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862620298P 2018-01-22 2018-01-22
US62/620,298 2018-01-22
PCT/US2019/014512 WO2019144107A1 (en) 2018-01-22 2019-01-22 Methods and compositions for discrete melt analysis

Publications (1)

Publication Number Publication Date
KR20200115552A true KR20200115552A (ko) 2020-10-07

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KR1020207024174A Ceased KR20200115552A (ko) 2018-01-22 2019-01-22 별개의 용융 분석을 위한 방법 및 조성물

Country Status (9)

Country Link
US (2) US11390902B2 (https=)
EP (1) EP3743211A4 (https=)
JP (1) JP2021511056A (https=)
KR (1) KR20200115552A (https=)
CN (1) CN111629831A (https=)
AU (1) AU2019209444A1 (https=)
BR (1) BR112020014412A2 (https=)
CA (1) CA3088250A1 (https=)
WO (1) WO2019144107A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230100950A (ko) * 2021-12-29 2023-07-06 주식회사 한국인삼공사 배암차즈기를 판별하기 위한 단일염기다형성을 갖는 유전자 마커

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200115552A (ko) * 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
US11039089B2 (en) * 2019-03-27 2021-06-15 Indian Institute Of Technology Ropar Thermal imaging for identifying a defect in a material
JP2023508843A (ja) * 2019-12-03 2023-03-06 バイオファイア・ダイアグノスティクス，リミテッド・ライアビリティ・カンパニー 正規化されたｔｍ値を使用するアッセイにおいて分析物を同定するためのシステムおよび方法
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
EP4402643A1 (en) * 2021-09-14 2024-07-24 Combinati Incorporated Systems and methods for polymerase chain reaction quantification
WO2023108014A1 (en) * 2021-12-07 2023-06-15 Luminex Corporation Methods and compositions for nucleic acid analysis
JP2025518761A (ja) * 2022-05-31 2025-06-19 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド 抗菌薬感受性を測定する方法及びキット
EP4709880A2 (en) * 2023-05-12 2026-03-18 Life Technologies Corporation Systems and methods for multiplexed polymerase chain reaction processes and data analysis
WO2025179142A1 (en) * 2024-02-22 2025-08-28 Cepheid Maximizing optical channel capabilities for target detection

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
JP3963422B2 (ja) * 2000-08-03 2007-08-22 日鉄環境エンジニアリング株式会社 核酸の測定方法
US7058184B1 (en) 2003-03-25 2006-06-06 Robert Hickling Acoustic measurement method and apparatus
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
EP2140033B1 (en) * 2007-03-28 2012-10-10 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
EP2291539B1 (en) * 2008-06-25 2018-03-14 Okura, Michael Apparatus for melting curve analysis of nucleic acids in microarray format
BRPI0917434A2 (pt) 2008-08-08 2015-12-01 Smiths Detection Inc algoritmo de detecçao para ensaio de pcr
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
JP2012511328A (ja) 2008-12-10 2012-05-24 スミスズ ディテクション インコーポレイティド 温度依存ハイブリダイゼーションを使用した核酸配列の同定及び区別
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
WO2010132813A2 (en) * 2009-05-15 2010-11-18 Idaho Technology, Inc. Systems and methods for automated melting curve analysis
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
WO2014028882A1 (en) 2012-08-17 2014-02-20 Bio-NEMS Corporation Nucleic acid classification
WO2015023616A2 (en) 2013-08-12 2015-02-19 The Johns Hopkins University Method for comprehensive, quantitative, and highly sensitive discrimination of nucleic acid sequences in homogeneous and heterogeneous populations
CN105765583B (zh) * 2013-08-30 2020-01-24 犹他大学研究基金会 去除dna解链分析中的荧光背景的量子方法
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP4613872A3 (en) * 2014-08-11 2025-11-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CA2970966A1 (en) * 2014-12-22 2016-06-30 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids
US9944978B2 (en) * 2014-12-30 2018-04-17 Telomere Diagnostics, Inc. Multiplex quantitative PCR
LT3298162T (lt) * 2015-05-18 2020-03-10 Saga Diagnostics Ab Tikslinės nukleorūgšties ir variantų aptikimas
EP3957398A1 (en) 2015-09-29 2022-02-23 Life Technologies Corporation Systems and methods for performing digital pcr
CN108368547B (zh) * 2015-12-15 2022-04-05 Seegene株式会社 与靶核酸序列有关的信号提取
US11232851B2 (en) 2016-06-23 2022-01-25 Canon Information And Imaging Solutions, Inc. System and method for modeling and subtracting background signals from a melt curve
US11034997B2 (en) 2016-09-15 2021-06-15 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
EP3299470B1 (en) 2016-09-23 2019-10-30 Roche Diagniostics GmbH A method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction
ES2909055T3 (es) 2016-11-17 2022-05-05 Combinati Incorporated Métodos y sistemas para el análisis y la cuantificación de ácidos nucleicos
KR20200115552A (ko) * 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230100950A (ko) * 2021-12-29 2023-07-06 주식회사 한국인삼공사 배암차즈기를 판별하기 위한 단일염기다형성을 갖는 유전자 마커

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BR112020014412A2 (pt) 2020-12-29
EP3743211A4 (en) 2021-11-24
AU2019209444A1 (en) 2020-07-30
WO2019144107A1 (en) 2019-07-25
CN111629831A (zh) 2020-09-04
JP2021511056A (ja) 2021-05-06
EP3743211A1 (en) 2020-12-02
US11390902B2 (en) 2022-07-19
US20190226008A1 (en) 2019-07-25
CA3088250A1 (en) 2019-07-25
US20220364148A1 (en) 2022-11-17

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