KR20200081553A - Composition for the prevention and improvement of Antitussive and Expectorant - Google Patents

Abstract

The present invention relates to a composition for preventing and improving antitussive and expectorant. The present application provides the composition containing a Isatis indigotica Fortune extract as an active ingredient, a manufacturing method thereof, and various uses. When the present application is disclosed, the pharmaceutical composition for treatment, improvement, and prevention of antitussive and expectorant can be usefully used in health functional foods. The pharmaceutical composition for the prevention or treatment of antitussive and expectorant contains the Isatis indigotica Fortune extract as the active ingredient.

Description

Composition for the prevention and improvement of Antitussive and Expectorant

The present application relates to the use of panram muscle extract, more specifically, this application relates to a composition for the prevention, treatment or improvement of antitussive expectorant, which includes panram muscle and cheonma extract as an active ingredient, and use thereof.

Cough is one of the body's important defenses, preventing various foreign substances from entering the airways.

Coughing is one of the common symptoms of a respiratory disease such as acute upper respiratory tract infection (cold), acute sinusitis, acute tonsillitis, etc.

When suffering from respiratory diseases, not only cough, but also sputum may accompany, and there is a need to develop a therapeutic agent that can effectively improve coughing and/or sputum.

0001) Republic of Korea Patent 10-2009-0025469 0002)Republic of Korea Patent 10-2009-0128156 0003)Korea Institute of Technology Patent 10-2011-0132407

One object of the present application is to solve the problems of the prior art described above, it is an object to provide a variety of uses of extracts containing panrameun.

Another object of the present application is to provide a health functional food for prevention or improvement of Jinhae expectorant, which contains Panram muscle extract as an active ingredient, in order to solve the above-mentioned problems of the prior art.

Another object of the present application is to provide a pharmaceutical composition for the prevention or treatment of antitussive expectorant ginseng containing panrameun extract as an active ingredient and a method for manufacturing the same, in order to solve the problems of the prior art described above.

Another problem of the present application, in order to solve the above-mentioned problems of the prior art, for preventing, treating, pharmaceutical composition or health functional foods of antitussive expectorant comprising panrameun extract as an active ingredient, an effective dose of the extract It is aimed at providing.

In addition to this, the present application includes all the possible uses related to the composition of the extract containing panram root, in order to solve the above-mentioned problems of the prior art.

In order to solve the above-mentioned problems of the present application, according to an aspect of the present application, a composition of an extract containing panram muscle is provided.

At this time, the extract containing panram muscle may include an extract using 50% ethanol.

The composition of the extract provided by the present application includes a pharmaceutical composition for the prevention or treatment of antitussive expectorant, which contains panram muscle as an active ingredient.

The composition provided by the present application may further include a cheonma extract.

In this case, when the composition is a mixed extract of the Cheonma extract and Panram muscle extract, it may be included in a weight ratio of 1-3. For example, in the case of panmulgeun and cheonma mixed extract, each weight ratio of 1:1; Or 1: 2 by weight; Or a weight ratio of 1 to 3, respectively; Or it may include at least one weight ratio selected from 2: 1 weight ratio.

The mixed extract may be a reflux extract of Panrameun and Cheonma, a polar solvent soluble extract or a non-polar solvent soluble extract, and the reflux extract is available from a solvent selected from water, methanol, ethanol, butanol, or a mixed solvent thereof, including purified water. It may be an extract, and the polar solvent soluble extract may be an extract soluble from a solvent selected from water, ethanol, butanol or a mixed solvent thereof, and the non-polar solvent soluble extract may be hexane, chloroform, dichloromethane or ethyl acetate. It can be a soluble extract. For example, it may be a reflux extract using a polar solvent, a hot water extract, an ethanol extract, in particular, it may be an extract using ethanol, specifically, it may be a reflux extract using 30-50% ethanol. Preferably, it may be a reflux extract using 50% ethanol.

The extract of the present application is a mixed extract from which the mixture is extracted after mixing the pangeun and cheonma, or the mixed extract is a mixture of the cheonma extract obtained from the cheonma and the pangeun extract obtained from the pangeun It can be an extract.

At this time, the extract of the composition may be repeatedly extracted 1 to 5 times for 1 to 24 hours at 20 ~ 150 ℃.

In order to solve the above-described problems of the present application, according to an aspect of the present application, a method for preparing an extract containing panram root is provided.

Method for producing an extract provided by the present application includes the steps of selecting panrameun (S100); Extracting an extract (S200); It includes the step of purifying the extract (S300); and drying the purified extract (S400). At this time, the extract, the purification method, may further include any one or more of the drying method.

In order to solve the other problems of the present application described above, according to another aspect of the present application, various uses of panram muscle extract are provided.

The extract provided by the present application may include any one or more uses, such as a pharmaceutical composition of the extract containing panram muscle, antitussive expectorant disease, health functional food, and the like.

In this case, the use may include a health functional food for the prevention or improvement of Jinhae expectorant, which contains Panram muscle extract as an active ingredient.

The health functional food may be any one or more selected from powders, granules, tablets, capsules, syrups, and beverages.

In addition, when using a mixed extract containing a cheonma extract and panrameun extract, the food may be included in a weight ratio of 1 to 3: 1 to 3, respectively.

According to the present application, the following effects occur.

First, the composition provided by the present application, comprising a panram muscle extract can provide an excellent antitussive expectorant improvement effect.

Second, the composition provided by the present application, the composition comprising panrameun extract can be provided as an active ingredient of a composition such as pharmaceuticals, processed foods, functional foods, food additives or functional beverages for the prevention and treatment of Jinhae expectorant .

Third, the composition provided by the present application, the composition containing panram muscle extract, for patients with antitussive or/and expectorant, unlike conventional temporary effects, relieves pain or discomfort, thereby reducing patient stress The effect can be provided.

1 is a view showing the results of sputum discharge experiments of Jeonho, Omija, McMoon-Dong, Apple Rock, Gil Kyung and Creation Extract.
Figure 2 is a diagram showing the results of sputum discharge experiments of extracts such as Jimo, Yonganyuk, Panguleun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jogu.
3 is a diagram showing the results of the antitussive activity of Jeonho, Omija, Panrameun, Cheonma, and Macmundong extracts.
4 is a diagram showing the results of the antitussive activity of Jimo, Omae, Yongan Yuk, Jogu, etc., and apple extract and creation extract.
5 is a diagram showing the results of sputum discharge experiments of two mixed extracts of Jeonho, Jeon, Cheonma, Omija, and Panrameun extracts.
6 is a diagram showing the results of the antitussive activity test of two mixed extracts of Jeonho, Jeon, Cheonma, Omija, and Panram muscle extracts.
7 is a view showing the results of sputum discharge experiments according to the mixing ratio of Panrameun and Cheonma complex extract.
8 is a view showing the results of the antitussive activity by the mixing ratio of Panrameun and Cheonma complex extract.
9 is a view showing the results of sputum discharge experiments by dose of mixed extracts in a ratio of 2 to 1 thousand and panrameun.
10 is a diagram showing the results of the antitussive activity test by dose of the mixed extract in the ratio of cheonma and pangeun 2:1.

The term "extraction" as used in this application refers to the process of separating a particular component using a solvent capable of dissolving the substance. The solvent includes, but is not limited to, polar solvents, non-polar solvents, and the like. The extraction method includes, but is not limited to, cold immersion, hot immersion, hot water extraction, fermentation extraction, and the like. The form of extraction used in the present application includes, but is not limited to, solid, liquid, and the like. The extraction, if necessary, may include a purification method, a concentration method, and the like. The purification method includes, but is not limited to, recrystallization, distillation, and chromatography methods. The concentration method includes, but is not limited to, evaporative concentration, reduced pressure concentration, freeze concentration, membrane separation and concentration.

The term "extract" used in the present application refers to a specific material obtained after performing the extraction process. The extract includes, but is not limited to, crude extract (Crude extract), hot water extract, fermented extract, fraction extract, and the like. The form of the extract includes, but is not limited to, solid, liquid, and the like.

The term "concussion" as used herein refers to the efficacy of stopping or/and calming and/or improving or/and inhibiting cough. The substance having antitussive action is called antitussives.

The term "sputum" used in the present application refers to the spit outwardly (for example, out of the mouth, etc.) as a secretion from various places of the respiratory system from the upper respiratory tract to the lungs. For example, it may be airway secretion, exudate from inflammation, airway secretion spit by cough, but is not limited thereto. The terms "sputum" or "sputum" or "sputum" are used interchangeably.

The term "Gundam" as used in this application refers to removing sputum or/and allowing it to drain well. The substance having the expectorant effect is called an expectant agent.

The term “containing” as used in this application means that the substance includes at least one other substance in the substance. For example, the meanings of'A substance contains B substance' and'B substance is contained in A substance' can be interpreted as meaning that B substance is contained in A. The terms "containing" or "including" are used interchangeably.

The term "improvement" used in the present application means that the existing state, function, effect, form, or the like is corrected, or a specific element is added and newly modified. The method for determining the improvement includes, but is not limited to, both objective methods and subjective methods such as experiments and surveys.

The term "Combination" used in the present application means mixing at least two substances or elements using appropriate conditions. The material or urea should also include a mixture of the same material or urea in different states. The state of the substance or urea includes, but is not limited to, solid, liquid, and the like. The conditions include, but are not limited to, ratio, specific gravity, weight, temperature, and the like. The terms "combination" or "mixed" or "composite" are used interchangeably.

The term "Effective" as used in the present application means a state in which a substance, an element, or the like exhibits a direct or indirect effect or action. The validity should also include values, quantities, etc. that affect the experiment. The material or urea should also include a mixture of the same material or urea in different states. The validity should also include those used to interpret the results obtained through physiological experiments, biochemical experiments, and the like. In addition, the effective effects and actions include, but are not limited to, increase, suppression, decrease, and increase. For example,'A substance is effective for B'means that A substance is effective for B. In addition, for example,'A substance has an effective amount of 1.0% in experiment B'means that substance A exhibits an effect at 1.0% in experiment B.

The term "treatment" as used in the present application means to alleviate a disease, disease, symptom, or the like, or inhibit its progress. The treatment includes, but is not limited to, physical treatment, drug treatment, psychological treatment, chemical treatment, and the like.

The term "health functional food" used in the present application means a food having functional properties useful for the human body by adding substances, urea, and the like to general food. The substance, urea should also include chemically, pharmaceutical, pharmacologically modified or processed materials. In addition, the functionalities include, but are not limited to, bioactive functions, psychological functions, and the like. The form of the food includes, but is not limited to, an auxiliary form, a drinking form, a ring form, and the like. The health functional food should also include the temporary functional effect.

In order to solve the above-mentioned problems of the present application, according to an aspect of the present application, a composition of an extract containing panram muscle is provided.

In order to solve the above-described problems of the present application, according to another aspect of the present application, a method for producing an extract containing panram muscle is provided.

In order to solve the above-described problems of the present application, according to another aspect of the present application, various uses of the extract containing panram muscle are provided.

Hereinafter, a composition, a manufacturing method, and various uses of the extract comprising the group consisting of pangeun and cheonma disclosed by the present application will be described in more detail.

1. Composition

The composition may include an extract.

The extract, leaves, roots, branches, fruits, seeds, etc. The extraction site may include any one or more or a combination thereof, but is not limited thereto.

The composition can inhibit or/and improve antitussive or/and expectorant.

The composition disclosed by the present application may include panram muscle extract.

i) Panam Geun ( Isatis indigotica Fort)

The pangeun belongs to the cruciferous family (Brassicaceae/Cruciferae), and the Latin name is Isatidis Radix. The panram muscle is columnar, twisted and slightly curved, and is 5 to 15 cm long and 3 to 8 mm in diameter. The outer surface is light brown. The rootstock is slightly thicker, and the spots with stems and leaves remain, and there are traces of vertical wrinkles, skin, and side roots. The vagina is brittle and the cross section is white and the neck is pale yellow. The panrameun is called Jeoncheonggeun, Namjeongeun, Jeongungeun, Daecheonggeun, Soongram.

The pangeun muscle is effective for removing blood heat from the blood, yangyeol detoxification (淸熱解毒) that lowers heat and removes poison, and phosphorus through the throat, headache, foot rash, and back pain , Antipyretic, analgesic, anti-inflammatory, antiviral and other effects have been reported.

The composition may further include a cheonma extract.

ii) Gastrodia elata Bl.

The cheonma belongs to the Orchidaceae family, and the Latin name is Gastrodiae Rhizoma. The cheonma is slightly curved and biased columnar-waterproof, 5-15 cm long, 2-5 cm wide, and 1-2 cm thick. The outer surface is pale yellow-white to yellowish brown with irregular vertical wrinkles and bumps. The vagina is hard and the curved side is yellowish-brown, glossy and keratinous. If you look at the transverse section of Cheonma under a microscope, you can see the calcium oxalate in the flow cell. The Cheonma is called Guidogwoo, Myeongcheonma, Suyangwoo, Jeokjeon, Jeongpungchogeun, Baekryongpi, etc.

The Cheonma is effective for stopping the surprised and stopping the cramps, etc., and is effective in the treatment of pneumonitis caused by the liver, and the head pains and dizziness Headache Hyunhun (頭痛眩暈), the effect of pediatric stiffness (小兒驚風) often seen in children, retardation, tetanus, anti-inflammatory and anti-asthma, etc. It becomes.

The composition may further include an extract selected from any one or more or combinations of other plant extracts, such as Omija, sage, and Jeonho.

The preceding genus belongs to the mountain family (Apiaceae/Umbelliferae), and the Latin name is Anthrisci Radix. The preceding call is spindle-shaped, and some stems and leaves are left at the root. The length is 3-10cm, the diameter is 7-15mm, and the outer surface is gray-black-brown, with horizontal wrinkles and root marks on the upper side. The curved side is grayish-white, and some are smooth, but some have a loose inside. This medicine has a unique aroma and a bit bitter taste.

The previous issue is sputum dynasty (이黄稠), where the yellowish wall is discharged, shortness of breath due to the heat of the fever, and Damyeol 10 million (痰熱喘滿), which is a symptom that the chest is full, and the wind fever invades the lungs and coughs while coughing. Haesudamda (風熱咳嗽痰多), the effect of lowering the flag and removing the wall has been reported.

The creation belongs to the family Asteraceae/Compositae, and the Latin name is Atractylodis Rhizoma. The creation is an irregularly curved columnar shape, 3-10 cm long and 10-25 mm in diameter. The outer surface is dark grayish brown-dark tan. The transverse section is almost circular and light brown to reddish brown spots can be seen. When this medicine is stored for a long time, white crystals sometimes precipitate.

The above creation is a dry and dry rain that strengthens the function of rain and dries out eczema, and it removes the wind and disperses the han. Eczema, swelling, sweating and other effects on the lower extremities have been reported.

The Omija belongs to the Magnoliaceae family, and the Latin name is Schizandrae Fructus. The Omija form an uneven spherical-spherical shape and have a dark red-brown color with a diameter of about 6 mm, wrinkles on the outer surface, and sometimes white powder. When the flesh is peeled, there are 1-2 seeds of kidney shape 2-5mm long, and the outer surface of the seed is glossy tan-dark reddish brown with clear seams on the back. Outer skin is easy to peel off, but inner skin is in close contact with endosperm.

Said Omiza is a true gene that treats the damage of the essence caused by a high fever, etc., anshin that stabilizes the mind, suhan to stop sweating, and decontamination itself to conspire and function of the gods.滋腎), Jigal (止渴) that stops thirst, Earth (구역) that stops the area, and Haeju-dok (毒酒주는) that relieves the poison caused by the excessive drinking by drinking alcohol. Dry mouth, old diarrhea, Kusaguri, dry-skinned body weight loss due to old-fashioned wounds, weight loss due to lungs, shortness of breath, and coughing due to cough Iii) The effects of such effects have been reported.

As an example of the present application, the extract may be extracted using an extraction solvent. The extraction solvent may be any one or more selected, such as a polar solvent, a non-polar solvent, but is not limited thereto.

The polar solvent refers to a liquid having an electric dipole fixed inside a molecule as a polar liquid and when used as a solvent, it is referred to as a polar solvent. Water, alcohol, acetone, etc. may be used as the polar solvent, but are not limited thereto. The water may include purified water, distilled water, and the like, but is not limited thereto. The alcohol has a hydroxy group (-OH group) as a functional group, and may include methanol, ethanol, propanol, isopropanol, butanol, etc., depending on the number of carbon atoms. I never do that.

The non-polar solvent refers to a solvent in which the solvent molecule does not have a permanent dipole and, as a result, cannot cause intermolecular association with polar species. Benzene, carbon tetrachloride, cyclohexane, etc. may be used as the non-polar solvent, but are not limited thereto. The benzene is an aromatic hydrocarbon, and the cyclohexane is synthesized by hydrogenation of benzene.

Alcohol, acetone, ether of 1 to 4 carbon atoms including purified water, methanol, ethanol, propanol, isopropanol, butanol, etc. as the extraction solvent , Benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and cyclohexane (cyclohexane) can be used alone or in mixture, but It is not limited.

As another example of the present application, the extraction solvent is, for example, 20 to 100%, 20 to 95%, 20 to 90%, 20 to 85%, 20 to 80%, 20 to 75%, 20 to 70%, 20 It may be used in a concentration of ~65%, 20~65% or 20-60%, but is not limited thereto. More specifically, the extraction solvent may be used at a concentration of 20-60%. Preferably, the extraction solvent may be used at a concentration of 50%.

As another example of the present application, the content of the extraction solvent may include any one or more, such as 1 to 10 times, 2 to 9 times, 3 to 7 times, or 4 to 6 times the content of the plant, but is not limited thereto. Does not. More specifically, the content of the extraction solvent may be performed 5 to 10 times.

As an example of the present application, the extract of the present application is a mixed extract from which the mixture is extracted after mixing the pangeun and cheonma, or the mixed extract is obtained from the cheonma extract and the pangeun obtained from the cheonma It may be a mixture of panrameun extract, but is not limited thereto.

The method for extracting the extract may include, but is not limited to, any one or more combinations of cold immersion, vacuum extraction, shaking extraction, reflux extraction, and hot water extraction. More specifically, the extraction method may be performed by a reflux extraction method.

In addition, the extract may include hot water extract, immersion extract, crude extract, distilled extract, ethanol extract, reflux extract, etc., but is not limited thereto. The extract may include any one or more or a combination of these, such as diluents and tablets.

In addition, the extract may also include an extract prepared by a drying process such as freeze drying, high temperature heating drying, natural drying, and freeze drying, but is not limited thereto.

In addition, the extract may also include an extract prepared by a purification process such as column chromatography, thin layer chromatography, high performance liquid chromatography, but is not limited thereto. Does not.

The extract of the present application may include any one or more or a combination thereof such as extraction, drying, and purification.

In one embodiment of the present application, it may be an extract containing the pangeun contained in the composition as an active material. The composition comprising the panram muscle extract as an active substance induces an inhibitory or/and improvement effect of antitussive or/and expectorant.

In another embodiment of the present application, it may be an extract comprising the pangeun and cheonma contained in the composition as an active material. The composition comprising the pangeun extract and Cheonma extract as an active material induces an inhibitory or/and improvement effect of Jinhae or/and expectorant.

The results of the expectorant effect of the composition comprising the extract of panrameun and cheonma as an active material can be confirmed through FIGS. 1 and 2.

1 and 2 include various herbal extracts such as Jeonho, Omija, McMundong, Apakrak, Gilkyung, Creation, Jimo, Yonganyuk, Pangeun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jojo, etc. as active substances. It is a figure showing the result of the expectorant effect of the composition.

In FIGS. 1 and 2, 50% ethanol is extracted from the above-mentioned mice by extracting 50% ethanol of the above-mentioned Jeonho, Omija, Macmundong, Apakrak, Gilkyung, Creation, Jimo, Yonganyuk, Pangeungeun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jojo. The result of measuring the amount of sputum discharge by quantifying the amount of phenol red discharged into the airways after administration at /kg is shown.

In particular, Figure 1, Jeonho, Omija, McMoon-Dong, Apple Lak, Gilgyeong and the results of the analysis of the geodam discharge efficacy of the extract. The NH ₄ Cl administration group is the control group, and the experimental group is the administration group of Jeonho, Omija, Pamo, McMoon-Dong, Apple Lak, Gilkyung, and Creation Extract. It can be seen that the sputum discharge efficacy is superior to that of the NH ₄ Cl-administered group in the order of McMoon-Dong and Apple-Rock.

In addition, Figure 2, Jimo, Yonganyuk, pangeun, cheonma, loquat, lily, Omae, crust, raw paper and coarse extract extract analysis results. The NH ₄ Cl administration group is a control group, and the experimental group is an extract administration group such as Jimo, Yonganyuk, Pangeun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jojo. It can be seen that the sputum discharge efficacy is superior to that of the NH ₄ Cl-administered group in the order of panramgeun, cheonma, and jimo.

1 and 2, it can be seen that among the various herbal extracts, herbal extracts having excellent sputum ejection efficacy are Panrameun and Cheonmi. Therefore, it can be said that the sputum discharge effect is generated through the phenol red discharge method by administering the panram muscle and cheonma extract to the mouse.

The results of the antitussive effect of the composition comprising the extract of panrameun and cheonma as an active material can be confirmed through FIGS. 3 and 4.

3 and 4 is a diagram showing the results of the antitussive effect of the composition containing various herbal extracts as active substances, such as Jeonho, Omija, Panrameun, Cheonma, Macmundong, Jimo, Omae, Yonganyuk, Jogu, etc. to be.

In FIGS. 3 and 4, 50% ethanol is extracted from the Jeonho, Omija, Panrameun, Cheonma, McMoondong, Jimo, Omae, Yonganyuk, Jogu, etc., and guinea pigs are sprayed with respiratory organs at 200mg/kg to induce coughing. By measuring the number of coughs for 15 minutes, the results of the antitussive activity effect are shown.

Particularly, FIG. 3 is an analysis result of antitussive activity of Jeonho, Omija, Panrameun, Cheonma and Macmundong extracts. The group administered with the mixed extract of Hwangnyeon and Ivy is the control group, and the experimental group is the group administered with the extracts Jeonho, Omija, Panramgeun, Cheonma, and Macmundong. It can be seen that the antitussive activity efficacy is superior in the order of Cheonma, Jeonho, and Panramgeun than the control group. In particular, it can be seen that Panramgeun and Cheonma show antitussive activity efficacy similar to Jeonho, which is known to have an effect on Jinhae or/and expectorant.

In addition, Figure 4, jimo, Omae, Yonganyuk, jogudeung, apple rock and anti-tussive activity analysis results of the resulting extract. The group administered with the mixed extract of Hwangnyeon and Ivy is the control group, and the experimental group is the group administered with Zimo, Omae, Yonganyuk, Jogu, etc., and apple extract. It can be seen that the antitussive activity efficacy of creation is superior to that of the control group.

3 and 4, it can be seen that among the various herbal extracts, herbal medicines superior to the known antitussive activity efficacy are cheonma and panrameun. Therefore, it can be said that the antitussive activity efficacy is generated through the method of measuring the number of coughs by spraying the extracts of panrameun and cheonma on guinea pigs.

Through the above Figures 1 to 4, since the extracts of Jeonho, Creation, Cheonma, Schisandra chinensis, and Panramgeun are excellent in Jinhae and Geodam, Jinhae of the composition comprising a mixed extract of two of the five herbal medicines as an active substance and The expectorant effect can be confirmed through FIGS. 5 and 6.

5 and 6 are diagrams showing the antitussive and expectorant effects of a composition comprising an extract of Jeonho, Chunma, Omija, Panrameun as an active substance.

In FIG. 5, 50% ethanol was extracted by combining two of Jeonho, Chunma, Cheonma, Omija, and Panrameun, and administered to the mouse at 200mg/kg, quantified the amount of phenol red discharged into the airways, and sputum discharged. The results of measuring the quantity are shown.

In particular, Figure 5 is the results of the analysis of the geodam discharge efficacy of a combination of two of Jeonho, creation, Cheonma, Omija, Panrameun. The NH ₄ Cl administration group is a control group, and the experimental group is a mixed extract of omnivory and omija (named as omni+mija), a mixed extract of creation and omija (named creation + omija), and a mixed extract of cheonma and panmigeun (named cheonma+panimeun) Administration group. It can be seen that the sputum discharge efficacy of Cheonma + Pangeun muscle is superior to that of the NH ₄ Cl administration group.

In FIG. 6, 50% ethanol was extracted by combining two of Jeonho, Chunma, Cheonma, Omija, and Panrameun and sprayed into the guinea pig at 200mg/kg to induce coughing to induce coughing. The results of the measurements are shown.

Particularly, FIG. 6 is an analysis result of antitussive activity efficacy of a mixed extract obtained by combining two of Jeonho, Yeonghwa, Cheonma, Omija, Pamo, and Panramgeun. The group administered with the mixed extract of Hwangnyeon and Ivy is the control group, and the experimental group is the mixed extract of Jeonho and Schisandra chinensis (named as Jeonji + Omija), the mixed extract of Schisandra chinensis and Schisandra chinensis, named Cheonma and Panmigeun extract (Cheonma+Pangeungeun) Name) It is the administration group. It can be seen that the antitussive activity efficacy of Cheonma + Panguleun is superior to that of the control group.

Referring to FIGS. 5 and 6, it can be confirmed that the mixed extract having excellent antitussive and expectorant efficacy among the mixed extracts combining two of Jeonho, Chunma, Cheonma, Omija, and Panrameun is a mixed extract of Cheonma + Panrameun. Therefore, it can be said that the Cheonma + pangeun mixed extract produces Jinhae and Geodam efficacy.

5 to 6 of the preceding, creation, Cheonma, Omija, pangeun of the composition containing a combination extract of Cheonma + Pangeun muscle as an active material has excellent antitussive and expectorant effects of the Cheonma + Pangeun muscle mixed extract The results of the effective mixing ratio for Jinhae and Geodam are shown in FIGS. 7 and 8.

FIG. 7 is a diagram showing the expectorant effect of a composition according to a blending ratio that includes a mixture of Cheonma + Panguleun as an active substance.

In FIG. 7, the amount of phenol red discharged into the airways after quantifying the amount of phenol red discharged into the airways by administering 200 mg/kg to the mouse and extracting 50% ethanol from the blended extracts of cheonma and pangeun respectively, respectively The results of measuring sputum emissions are shown.

Particularly, FIG. 7 shows the results of the analysis of the expectorant efficacy of the extract of Cheonma and Panrameun alone and the mixed extract of Cheonma + Panrameun. The NH ₄ Cl administration group was a control group, and the experimental group was a combination of cheonma and panram muscle extracts (1:0, 0:1) and cheonma + pangul muscle (3:1, 2:1, 1:1, 1:2) , 1:3). It can be seen that the sputum discharge efficiency is superior in the mixed extract of the ratio of 2:1, 1:1, and 1:2 of Cheonma + Panrameun than the extract of Cheonma and Panram muscle alone.

8 is a view showing the antitussive effect of the composition according to the blending ratio containing a mixture of Cheonma + pangeun as an active substance.

In FIG. 8, 50% ethanol is extracted by mixing the blending ratio of Cheonma and Panram muscle alone extract and Cheonma + Panram muscle and sprayed into the guinea pig at 200mg/kg to induce cough to measure the number of coughs for 15 minutes to measure the antitussive activity effect. The results of the measurements are shown.

In particular, FIG. 8 is a result of analysis of antitussive activity efficacy of a mixture extract of Cheonma and Panram muscle alone extract and Cheonma + Pangram muscle. The administration group of the mixed extract of Hwangnyeon and Ivy is a control group, and the experimental group is a group of Cheonma and Panram muscle extracts alone (named Cheonma and Panrameun) and a combination ratio of Cheonma+panrameun (named 2:1, 1:1 and 1:2). . It can be seen that the antitussive activity efficacy is superior in the mixed extract having a ratio of 2:1 of Cheonma + Panram muscle than the extract of Cheonma and Panram muscle alone.

Referring to FIGS. 7 and 8, it can be seen that the antitussive and expectorant effects are excellent in the 2:1 compounding ratio mixed extract of the blend extract according to the compounding ratio of Cheonma + pangeun. Therefore, it can be said that the mixed extract of the ratio of Cheonma+Pangeungeun 2:1 produces Jinhae and Geodam efficacy.

The results of the experiments on the antitussive and expectorant effects by dose of the Cheonma + Panguleun 2:1 mixed extract are excellent in the 2:1 mixed extract of the Cheonma + Pangul muscle compounding ratio through FIGS. 7 to 8. It is illustrated in FIG. 10.

FIG. 9 is a diagram showing the expectorant effect by dose of a composition comprising a mixed extract of Cheonma + pangeun 2:1 as an active material.

In FIG. 9, 50% ethanol was extracted from the mixed extract with a ratio of 1 to 2% of cheonma + pangeun and administered to the mouse at 200 mg/kg, and then the amount of phenol red discharged to the airways was quantified to measure the amount of sputum discharge. One result is shown.

In particular, Figure 9 is a result of analyzing the efficacy of the mixed extract of expectorant extract of the ratio of Cheonma + pangeun 2:1. The NH ₄ Cl administration group is the control group, and the experimental group is the administration group according to the compounding ratio dose (named 100, 200, 400, 800) of cheonma + pangeun 2:1. It can be seen that the sputum discharge efficiency is excellent as the capacity of the mixed extract in the ratio of Cheonma + pangeun 2:1 is increased. That is, it can be seen that the mixed extract of the cheonma + pangeun 2:1 compounding ratio is excellent in that dose-dependent sputum discharge efficiency increases.

10 is a view showing the antitussive effect of each composition of the composition comprising a mixture of Cheonma + pangeun 2:1 as an active substance.

FIG. 10 shows the results of measuring the antitussive activity effect by measuring the number of coughs for 15 minutes by inducing coughing by inhaling 50% ethanol by mixing at a ratio of 2:1 of Cheonma + pangeun, and extracting 50% ethanol to the respiratory system at 200 mg/kg. It is done.

Particularly, FIG. 10 is a result of analyzing the antitussive activity efficacy of the mixed extract of the Cheonma + pangeun 2:1 ratio. The administration group of the mixed extract of Hwangnyeon and Ivy is the control group, and the experimental group is the administration group according to the compounding ratio dose (named 100, 200, 400, 800) of Cheonma Cheonma + Panguleun 2:1. It can be seen that the antitussive activity efficacy is excellent as the capacity of the mixed extract in the ratio of Cheonma + pangeun 2:1 is increased. That is, it can be seen that the mixed extract of the ratio of Cheonma + pangeun 2:1 is excellent in dose-dependent antitussive activity.

Referring to FIGS. 9 and 10, it was confirmed that the mixed extract of Cheonma + pangeun at a 2:1 compounding ratio generated antitussive and expectorant effects as the dose increased. Therefore, it can be said that the mixed extract of the ratio of Cheonma + pangeun 2:1 not only produces antitussive and expectorant effects, but also dose-dependent effects of antitussive and expectorant.

The composition of the present application may include one or more of goitrin, arginine, beta-sitosterol, guanosine, uridine, adenosine, or a combination thereof. The goitrin, arginine, beta-sitosterol, guanosin, uridine, adenosine, etc. may act as active ingredients. The active ingredient may be derived from panram muscle extract.

The goitrin inhibits the iodine introduction step into the thyroxine precursor and at the same time has the effect of inhibiting the secretion of thyroxine.

The arginine is an essential amino acid that helps the pituitary gland to secrete hormones normally, and is known to promote nutrient transport, blood vessel expansion, and muscle relaxation.

The beta-sitosterol is a kind of phytosterols, and is abundantly contained in most plants, particularly rice bran, peanuts, legumes, pumpkin seeds, corn oil, radish, cabbage, goji berries, and the like. Beta-sitosterol reduces cholesterol in the blood and is known to be effective in treating prostatic hyperplasia in addition to anti-cancer, antioxidant, anti-inflammatory, hypoglycemic, and immune enhancing effects.

The guanosine is a kind of purine nucleoside and is one of skin constituents that play an important role in skin regeneration.

The uridine is one of the pyrimidine nucleotides and is a source of uracil, which is one of RNA constituents. The uridine acts to activate and maintain cell safety and homeostasis. In addition, the uridine is known to be effective in cell regeneration and energy metabolism.

The adenosine is one of the purine nucleosides and plays an important role such as constructing a gene of DNA and a neurotransmitter such as ATP and cAMP through phosphoric acid binding. In addition, the adenosine has effects such as central nerve suppression, wound recovery, and anti-inflammatory reaction.

The composition of the present application may further include any one or more of Vanillin, Vanillyalcohol, Ergothioneine, Gastrodin, Vitamin A, Gastrodigenin, or a combination thereof. The Vanillin, Vanillyalcohol, Ergothioneine, Gastrodin, Vitamin A, Gastrodigenin, etc. may act as active ingredients. The active ingredient may be derived from Cheonma extract.

Vanillin is 4-oxy-3-methoxybenzaldehyde. It exists as a glycoside in vanilla beans and firs. Vanillin is a sublimable, soluble in organic solvents, and is a vanilla flavor ingredient that adds the sweetness of food, and is widely used as a flavoring agent such as chocolate, ice cream, and candy. Vanillin has anti-angiogenic and anti-inflammatory activity, and this function is caused by inhibiting the formation of nitric oxide in cells.

Vanillyalcohol is one of the phenolic compounds, and has the function of inhibiting the anti-angiogenesis of cancer cells, the protection and treatment of brain nerves, and the like.

The Ergothioneine is a strong and stable oxidant as an amino acid, and has an antioxidant role, cell aging inhibition, and the like.

It is known that the Gastrodin prevents the invasion of calcium into the cell and lowers the viscosity in the blood to smooth the flow of blood and help the circulation of fine blood vessels such as capillaries. The Gastrodin is effective in antioxidant, cell aging inhibition, anti-inflammatory action, anticonvulsant action.

The vitamin A is a fat-soluble vitamin, and vitamin A strengthens the body's resistance. Biomembrane (membrane) It plays a role in regulating the structure and function of tissue, and promotes cell regeneration as an epithelial cell growth factor, protecting the mucous membranes of the oral cavity, airways, stomach and intestines. When vitamin A is deficient, epithelial cells gradually turn into hard, dry keratinous tissues, and the secretion function of mucus is lost, and bacteria are easily invaded. It also acts as an antioxidant that prevents cell oxidation. It is also required for the production of rhodopsin, a photosensitive pigment present in rod cells in the retina of the eye. Vitamin A deficiency impairs animal reproductive function.

Gastrodigenin is known to exhibit a pro-kinetic action in both the stimulation and acetic acid experiments of the mouse tail. The Gastrodigenin has sedative, anticonvulsant and other effects.

As described above, the present application can provide a composition comprising the panram muscle extract. In addition, the pangeun extract of the present application may further include a cheonma extract.

However, the combinations, ingredients, and the like are exemplary, and the scope of the present application is not limited to the above.

2. Manufacturing method

Hereinafter, a method for producing an extract containing panram muscle disclosed by the present application will be described in more detail.

In one embodiment of the present application,

Method for producing the extract disclosed by the present application,

Selecting a pangeun (S100); Extracting an extract (S200); It includes the step of purifying the extract (S300); and drying the purified extract (S400).

In the step (S100) of selecting the pangeun may further include a thousand horses can be selected.

The order of selecting the pangeun (S100) and extracting the extract (S200) can be crossed.

For example, the extract of the present application is a mixed extract from which the mixture is extracted after mixing the pangeun and cheonma, or the mixed extract is cheonma extract obtained from the cheonma and pangeun obtained from the pangeun It may be an extract, but is not limited thereto.

It is preferable to use the extracts processed by steps S300 and S400, but the extracts are not limited thereto.

The extracting of the extract solution (S200) may include general plant extraction pretreatment processes.

For example, the step (S200) of extracting the extract may include any one or more or a combination thereof, such as selecting a part of the plant, washing the plant, drying the plant, and cutting the plant. Can.

As an example of the present application, the step of selecting a part of the plant may be a previous step to maximize the efficacy of the extracted extract. For example, if the plant has efficacy only at the root, rather than using the entire area of the plant, the time for extraction can be saved, and the concentration effect of the extract can be provided. The plant part may include any one or more or combinations of leaves, branches, roots, fruits, and seeds. The cultivation period of the plant is not limited. In addition, the harvesting time of the plant is not limited.

As an example of the present application, the step of washing the plant may be a step of removing a foreign substance on the surface of the plant. For example, by minimizing residual substances such as fertilizer or pesticide used when growing the plant, an effect of increasing the purity of the extract may be provided. The washing method may include, but is not limited to, machine washing, wiping with a cloth, vacuum washing, hot air washing, or the like.

As another example of the present application, the step of drying the plant may be a step of concentrating components effective in the extract, or removing the solution used in the washing step. The drying method may include any one or more of natural drying, cold air drying, warm air drying, or a combination thereof, but is not limited thereto.

As an example of the present application, the step of cutting the plant may be a previous step of allowing the effective components to be better escaped from the extract. For example, since the surface area of the plant is increased according to the thickness or shape of cutting the plant, the contact surface with the solvent is increased, so that the effect of extracting the active ingredients can be provided in the step of extracting the extract. The method for cutting may include any one or more or a combination thereof, such as an ultrafine particle cutting method, a cropping method, a circular cutting method, or a right angle cutting method. The shape of the cut may include any one or more or a combination thereof such as a knit piece, a knit piece, or a lump piece, but is not limited thereto.

As an example of the present application, the step of extracting the extract solution (S200) is a step of extracting an active ingredient using a solvent. The form of the active ingredient may include any one or more or a combination of solid, liquid, and gas. The solvent may include any one or more of water, an organic solvent, an inorganic solvent, or a combination thereof.

In some embodiments of the present application, the organic solvent may include any one or more or a combination of ethanol (Ethanol, EtOH), methanol (Methanol, MeOH), acetone, xylene, and the like. More specifically, ethanol may be used as the organic solvent.

In some embodiments of the present application, the purity of the organic solvent may include, but is not limited to, any one or more of carbon, carbon, 2, 3, 4, 5, etc. .

As an example of the present application, the concentration of the solvent is 30 to 100%, 30 to 95%, 30 to 90%, 30 to 85%, 30 to 80%, 30 to 75%, 30 to 70%, 30 to 65% Or 30-60%, or any combination thereof. More specifically, the concentration of the solvent can be performed at 50%.

As an example of the present application, the content of the solvent may include any one or more or a combination of 1 to 10 times, 2 to 9 times, 3 to 7 times, or 4 to 6 times the content of the plant. More specifically, the content of the solvent may be performed 5 to 10 times.

As another example of the present application, the method for extracting the extract may include any one or more or a combination thereof, such as a cold immersion method, a reduced pressure extraction method, a shaking extraction method, a reflux extraction method, and a hot water extraction method. More specifically, the extraction method may be performed by a reflux extraction method.

In some embodiments of the present application, the method of extracting the extract may perform 1 to 5 repetitive extractions at 20 to 150° C. for 1 to 24 hours. More specifically, the method of extracting the extract may be performed by performing a second repeated extraction at 80°C for 3 hours.

As an example of the present application, the step of purifying the extract (S300) is a step for removing impurities and increasing the content of the active ingredient. The method of purifying the extract may include any one or more or a combination thereof, such as a centrifugation method, a filtration method, a sedimentation method, an ethanol precipitation method, a water precipitation method, and a chromatography method. More specifically, it can be carried out by the filtration method. The form for purifying the extract may include any one or more or a combination of solid, liquid, and gas.

The step of purifying may further include a concentration method. The concentration method may include an evaporative concentration method, a reduced pressure concentration method, a freeze concentration method, a membrane separation concentration method, and the like. More specifically, it can be performed by the reduced pressure concentration method.

As an example of the present application, the step of drying the extract (S400) is a step of concentrating the active ingredient of the extract and transforming it into an easy-to-use form. The dry form may include any one or more or a combination of solid, liquid, and gas, but is not limited thereto. The drying method may include any one or more or a combination of tunnel type oven drying, vacuum drying, spray drying, freeze drying, and far infrared drying, but is not limited thereto.

As an embodiment of the present application, it may be a mixed extract comprising the pangeun and cheonma. Preferably, the weight ratio of the mixed extract of Cheonma and Panrameun is 1:1, 1;2, 1:3. 2:1, 3:1 or 3:2, but is not limited thereto. Preferably, in the case of the cheonma and panrameun it can be carried out in a weight ratio of 1:1, 1:2 or 2:1. Even more preferably, it can be carried out at a weight ratio of 2:1 in the case of cheonma and panrameun.

The order of administration of the mixed extract prepared as described above is not limited.

As another embodiment of the present application, the mixed extract may include an extract extracted with the solvent. More specifically, it may include a mixture of panrameun and cheonma. The solvent may include any one or more of water, an organic solvent, a base solvent, or a combination thereof. Specifically, the solvent may be performed with an organic solvent. Specifically, the volume of the mixed extract and the solvent may be performed 1 to 30 times. Preferably, the volume of the solvent may be performed 5 to 10 times the volume of the mixed extract.

As another embodiment of the present application, the method for extracting the mixed extract with the solvent may include any one or more of a combination of hot water extraction, reflux extraction, and heat extraction. More specifically, the method of extracting with the solvent may be performed by reflux extraction.

The order of administration of the mixed extract and the solvent prepared as described above is not limited.

The method of extracting with the solvent may further include any one or more or a combination thereof, such as a purification method and a drying method.

In some embodiments of the present application, the purification method may include a centrifugation method, a chromatography method, a filtration method, etc., but is not limited thereto. Preferably, it can be carried out by filtration.

In some embodiments of the present application, the drying method may include freeze drying, natural drying, cold air drying, and the like, but is not limited thereto.

As described above, the present application may include a method for preparing the panram muscle extract. In addition, the present application may include a method for preparing the panram muscle extract and cheonma extract.

However, the manufacturing method, weight ratio, and the like are exemplary, and the scope of the present application is not limited to the above.

3. Applications

Hereinafter, various uses of the panram muscle extract disclosed by the present application will be described in more detail.

The present application may include any one or more or a combination of pharmaceutical compositions, inflammatory disease compositions, and health functional food compositions of the extract containing panram muscle. At this time, the extract containing panrameun may further include a cheonma extract.

[Pharmaceutical composition]

An example of the present application is a pharmaceutical composition including a composition and a manufacturing method comprising the extract containing the panram muscle as an active ingredient described above and various uses thereof.

Another example of the present application is a pharmaceutical composition including a composition and a manufacturing method comprising the pangeun extract and Cheonma extract as active ingredients, and various uses thereof.

The pharmaceutical composition is composed of essential components that improve inflammation.

Inflammation is a condition in which biological tissues, such as acute inflammation and chronic inflammation, are irritated, symptoms, and diseases.

For example, the inflammation improving component of the present application may improve inflammation of the respiratory system.

For a specific example, the inflammation improving component of the present application may improve antitussive or/and expectorant.

The inflammation improving component may be a composition and a manufacturing method comprising the extract as an active ingredient.

Depending on the composition of the inflammation-improving component, the aspect of the pharmaceutical composition may vary.

The pharmaceutical composition may have different aspects of the pharmaceutical composition depending on the subject. The subject may include any one or more of plants, animals, or a combination thereof. In addition, depending on the subject, the dosage of the pharmaceutical composition may vary.

To largely classify the aspect of the pharmaceutical composition, it can be divided into solid formulations, gel formulations, gel-spray formulations, capsule formulations, and the like. However, this classification is not essential and the present application is not limited thereto. In addition, the pharmaceutical composition may further include additives such as excipients, diluents, and preservatives for stability and convenience, but is not limited thereto.

The additive may include any one or more or a combination of xylitol, malitol, starch, acacia, rubber, gelatin, magnesium, sorbitol, mannitol, sodium benzoate, and the like.

The pharmaceutical composition may vary in dosage, frequency, and method of administration of the pharmaceutical composition according to the inflammation. The dosage, the frequency of administration, and the method of administration are not limited. However, it is preferable to use appropriately according to the type of inflammation, progression, and the like.

The pharmaceutical composition of the present application can be used alone for improving inflammation, treatment, prevention, etc., but can be used in combination with surgery, biological treatment, chemical treatment, and the like.

[Jinhae Gundam]

An example of the present application is the prevention, treatment and/or improvement of antitussive expectorant comprising a composition and a manufacturing method comprising the above-described extract containing panrameun and cheonma as an active ingredient.

The Jinhae geodam may include any one or more of coughing, sputum, sputum, sneezing, or a combination thereof.

The composition and manufacturing method comprising the extract containing panram muscle as an active ingredient may be used for treatment or/and prevention or/and improvement of any one or more or a combination thereof, such as coughing, sputum, sputum, and sneezing.

In some embodiments of the present application, the effect of antitussive or/and expectorant in a mouse or/and guinea pig can be confirmed with an extract comprising any one or more of the above-mentioned cheonma and pangeun. The higher the concentration of the cheonma or/and panram muscle extract, the better the effect of Jinhae or/and expectorant.

In one embodiment of the present application, the effect of Jinhae or/and expectorant may be more effective when the ratio of Cheonma and Panram muscle extract is 1 or more selected from 1:1, 1:2, and 2:1. In particular, when the ratio of cheonma and panram muscle extract is 2:1, the efficacy of Jinhae or/and expectorant in the mouse or/and guinea pig may be the best.

In one embodiment of the present application, when the extract containing any one or more of the cheonma and pangeun or a combination thereof is a 50% ethanol extract, the effect of Jinhae or/and expectorant may occur. In particular, when 50% ethanol is extracted in the ratio of cheonma and pangeun at 2:1, the effect of antitussive or/and expectorant can occur in mice or/and guinea pigs.

As described above, the present application is an inflammatory disease comprising a composition and a manufacturing method comprising an extract containing any one or more of the cheonma and panram muscle as an active ingredient, in particular, the respiratory inflammatory disease more specifically Jinhae or/and expectorant can be used for prevention, treatment and/or improvement.

[Healthy functional food composition]

An example of the present application is the use of a health functional food for preventing, treating, and/or improving antitussive expectation, which includes a composition and a manufacturing method comprising the above-mentioned extract containing panram muscle as an active ingredient.

The Jinhae geodam may include any one or more of coughing, sputum, sputum, sneezing, or a combination thereof.

The health functional food may further include an invalid ingredient generated in the extraction process using the extract.

The health function may include, but is not limited to, all of the general purposes for the purpose of promoting the health of the human body, health use, and the like.

The health promotion of the human body may include, but is not limited to, physical, biochemical, biological, physiological, immunological, therapeutic, psychological, etc., which are general health promotion.

The health functional food may include, but is not limited to, all of the conventional ones to which useful nutrients, functional ingredients, and the like have been added according to the above purpose.

Types of health functional foods may include any one or more of vitamins, nutritional supplements, candy, snacks, powders, tablets, capsules, beverages, or a combination thereof.

When manufacturing a dietary supplement, it may include any one or more or a combination of additives, adjuvants, and oxidizing agents.

The health functional food may include, but is not limited to, all the common effects of promoting health function regardless of the period of taking the food, such as short-term or long-term consumption.

Health functional foods may be taken with any one or more or combinations of medicines, supplements, etc., but are not limited thereto.

When manufacturing the dietary supplement of the present application, it is prepared by essentially including the extract. However, the ratio and content of the extract are not limited.

Most health functional foods can have negative effects such as half effect, inhibition, and suppression of health functional effects due to the action of chemical components.

However, when manufacturing the health functional food of the present application, since the extract is a natural component, it may help to improve the health function.

Natural ingredients have little side effects even when taken for a long time.

As described above, the present application can be used for preventing, treating and/or improving antitussive or/and expectorant compositions and methods of preparing the extract containing panram muscle as an active ingredient.

[Example]

Hereinafter, the present application will be described in more detail through examples.

One embodiment of the present application relates to the manufacture of the extract for respiratory inflammatory disease, more specifically, Jinhae or/and expectorant, and sputum discharge ability, antitussive effect, etc., but these examples are only intended to illustrate the present application, It will be apparent to those skilled in the art that the scope of the present application is not to be construed as limited by these examples.

Example 1: Candidate substances

For selection of candidate substances with excellent efficacy in Jinhae and Gundam, Jeonho, Omija, McMun-Dong, Apple Lak (Susemioi), Gilkyung, Creation, Jimo, Yonganyuk, Pangeungeun, Cheonma, Non-Leaf, Lily, Omae, Crust, Wonji, Jogu, etc. The efficacy of Jinhae and Geodam was tested through the preparation of 16 extracts.

Example 2: Preparation of extract

20 g of each of the candidate substances of Example 1 was weighed, and 200 ml of 50% ethanol aqueous solution, which was 10 times the weight of the sample, was added and refluxed for 2 hours and 3 times at 80° C., and the obtained extract was filtered and then concentrated under reduced pressure. And it was used as a sample in this experiment while being stored in a refrigerator in powder form.

Example 3: Antitussive activity measurement

In order to measure the antitussive activity of the candidate substances extracted in Example 2, the guinea pig control and sample administration groups were divided into two groups. In the guinea pig control group, saline was administered orally at 10 ml per kg, and in the sample administration group, the extract of Example 2 was orally administered at 10 mg/kg at a concentration of 200 mg/kg, respectively, and the sample was prepared by dissolving in saline. After 1 hour of oral administration, guinea pigs were placed in a plethysmograph chamber (Buxco, U.S.A) to induce stability, and coughing agents (citric acid, citric acid) were sprayed to induce coughing. Guinea pigs were exposed to 0.2-0.4M citric acid for 10 minutes, and the cough water generated at this time was measured. After stopping the spraying of citric acid, 5 minutes were measured.

Example 4: Measurement of sputum discharge effect

In order to confirm the sputum discharge effect of the candidate substances extracted in Example 2, ICR mice were divided into three groups, each of a normal group, a positive control group, and a sample administration group. In the normal group, saline was administered orally 10 ml per kg, and in the positive control group, ammonium chloride (NH ₄ Cl) was administered orally 10 ml per kg at a concentration of 1500 mg/kg. In the sample administration group, the extract of Example 2 was orally administered at 10 mg/kg at a concentration of 200 mg/kg, respectively, and the sample and NH4Cl were dissolved in saline to prepare. Each group was given an intraperitoneal injection of 500 mg/kg of 5% phenol red after 30 minutes of oral administration. Mice in each group were over-anesthetized with CO ₂ after 30 minutes, opened, cut off the abdominal aorta and bleeded, and the entire trachea was excised. The separated organ was placed in 1 ml saline, sonicated for 10 minutes, and then centrifuged (4,000 rpm, 5 minutes, room temperature). 0.9 ml was taken from the separated supernatant, and 0.09 ml of 1N NaOH was added to the solution to develop color. The absorbance was measured at 540 nm to measure the amount of phenol red extracted from the airways.

Example 5: Selection of candidate substances (alone) with excellent antitussive effect

In order to select candidate substances having excellent efficacy in antitussive and expectorant among 16 candidate substances of Example 1, antitussive and expectorant effects of each candidate substance were measured by the methods of Examples 2 to 4.

1 and 2 in the composition including various herbal extracts, such as Jeonho, Omija, McMundong, Apple Rock, Gilkyung, Creation, Jimo, Yonganyuk, Panramgeun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jojo, etc. The results of the expectorant effect are shown. Also via a one can see maekmundong, the sputum discharge efficacy superior to apple lock order than NH ₄ Cl group, and, to determine the degree the sputum discharge efficacy superior to plate ramgeun, Gastrodia elata, jimo order than NH ₄ Cl group with 2 there was.

That is, it was confirmed through FIG. 1 and FIG. 2 that among the various herbal extracts, the herbal extract having the best sputum discharge efficacy was Panrameun.

In Figures 3 and 4, Jeonho, Omija, McMundong, Apple Rock, Gilkyung, Creation, Jimo, Yonganyuk, Panrameun, Cheonma, Loquat, Lily, Omae, Crust, Wonji and Jojo, etc. The results of the antitussive effect of are shown. 3 and 4, it can be seen that, compared to the control group hwangnyeon and ivy mixed extracts, the test group Jeonho, Omija, Panramgeun, Cheonma, and Macmundong extract administration groups had superior activity efficacy due to the fact that Cheonma, Jeonho, Panramgeun were concentrated. In particular, it can be seen that Panramgeun and Cheonma show antitussive activity efficacy similar to Jeonho, which is known to have an effect on Jinhae or/and expectorant.

In other words. 3 and 4, it can be confirmed that among the various herbal extracts, herbal medicines superior to the known antitussive activity efficacy are cheonma and panrameun.

Example 6: Selection of candidate substances (combinations) with excellent antitussive effects

In addition, the present inventors further tried to further confirm whether the extract of Cheonma was combined with the extract of Panmageun, which is excellent in antitussive and expectorant, to show an excellent antitussive expectorant effect.

In particular, compared to the combination of two or more of the previous call, creation, and Omija, which is known to have an effect on the conventional Jinhae Geodam, it was confirmed that the extract of Panrameun and Cheonma of the present invention has a better Jinhae Geodam effect.

After weighing 20 g of each of the candidate substances, 200 ml of 50% ethanol aqueous solution, which is 10 times the weight of the sample, was added and refluxed for 2 hours and 3 times at 80° C., and the obtained extract was filtered and mixed in two ways necessary for the experiment. It was concentrated under reduced pressure and used as a sample in this experiment while being stored in a refrigerator in powder form.

The results are shown in FIGS. 5 and 6.

5 and 6 show the results of the antitussive and expectorant effects of the composition comprising the extracts of Jeonho, Jeonma, Cheonma, Omija, and Panrameun as active substances. Figure 5 shows the results of the analysis of the expectorant efficacy of the mixed extract of the combination of two of Jeonho, creation, Cheonma, Schisandra chinensis, and Panrameun, compared to the control group NH ₄ Cl administration group Cheonma and Panram muscle mixed extract (named Cheonma + Pangeun) It was confirmed that the efficacy of sputum discharge was excellent. 6 is a result of analyzing the antitussive activity of the mixed extracts of the two extracts of Jeonho, Creation, Cheonma, Schisandra chinensis, Panrameun, compared to the control group Hwangnyeon and Ivy mixed extract administration group, the experimental group Jeonho and Omija mixed extracts (Gunho+Omija) (Named as)), Creation and Omija mixed extract (Creation + Omija), Cheonma and Panram muscle extract (named as Cheonma + Panmeun) Cheonma and Panram muscle extract (named as Cheonma + Pangeun) in the administration group It was confirmed that the efficacy was excellent.

Example 7: Antitussive expectorant effect by blending ratio of Cheonma and Panram muscle mixed extract

In order to know the blending ratio of Panrameun and Cheonma mixed extract having the best antitussive expectorant effect, antitussive and expectorant effects according to the specific compounding ratios shown in FIGS. 7 and 8 were measured.

Figure 7 shows the results of the expectorant effect of the composition by composition ratio containing a mixture of Cheonma + pangeun muscle extract as an active material. 7, the control group, compared to the NH ₄ Cl administration group, the experimental group of cheonma and panram muscle alone extracts (1:0, 0: 1) and cheonma + panram muscle combination ratio (3:1, 2:1, 1: Named 1, 1:2, 1:3) Among the administration groups, sputum discharge efficacy was superior in the mixed extract of the ratio of 2:1, 1:1, 1:2 of Cheonma+Pangeungeun than the extract of Cheonma and Panram muscle alone. I could confirm.

8 shows the results of the antitussive effect of the composition according to the blending ratio containing the mixed extract of Cheonma + pangeun as an effective substance. 8, the control group compared to the group administered with a mixture of Hwangnyeon and Ivy, the experimental group of Cheonma and Panrameun alone extract (named Cheonma, Panrameun) and Cheonma + Panrameun blend ratio (2:1, 1:1, 1:2) Named as) It was confirmed that the antitussive activity efficacy was superior in the mixed extract having a ratio of 2:1 of Cheonma+Pangeungeun than the extract of Cheonma and Pangeun alone among the administration groups.

Referring to FIGS. 7 and 8, it can be seen that the antitussive and expectorant effects are excellent in the 2:1 compounding ratio mixed extract of the blend extract according to the compounding ratio of Cheonma + pangeun. Therefore, it can be said that the mixed extract of the ratio of cheonma + pangeun 2:1 is the most excellent antitussive and expectorant efficacy.

Example 8: Antitussive expectorant effect of Cheonma and Panrameun 2:1 mixed extract by dose

Through Example 7, the antitussive and expectorant effects were excellent in the 2:1 mixed extract of the Cheonma and Panram muscle mix ratios.

FIG. 9 shows phenol red (phenol red) discharged into the airways after administration of 200 mg/kg to the mouse by extracting 50% ethanol from a mixed extract of a 2:1 compounding ratio of cheonma and pangeun (hereinafter referred to as cheonma + pangeun). The result of measuring the amount of sputum discharge by quantifying the amount is shown. Through FIG. 9, the control group dose group (named 100, 200, 400, 800 mg/kg) is administered to the experimental group than the control group NH ₄ Cl administered by the test group, Chunma + pangeun 2:1. It was found that the sputum discharge efficiency was excellent as the capacity of the mixed extract in the ratio of cheonma + pangeun 2:1 was increased. That is, it can be seen that the mixed extract of the cheonma + pangeun 2:1 compounding ratio is excellent in that dose-dependent sputum discharge efficiency increases.

Figure 10 is a mixture of Cheonma + pangeun 2:1 ratio of 50% ethanol extracted by guinea pig by dose (100, 200, 400, 800mg / kg) by spraying the respiratory system to induce cough and measure the number of coughs for 15 minutes The results of measuring the Jinhae activity are shown. Through FIG. 10, the composition ratio of Cheonma Cheonma+Panguleun 2:1, which is the experimental group, by the dose group (named 100, 200, 400, 800) compared to that of the control group of the Hwangnyeon and Ivy mixed extract as a control group, is a combination ratio of Cheonma+Pengeun 2:1 in the administration group. It can be seen that the antitussive activity efficacy is excellent as the capacity of the mixed extract of the mixture increases. That is, it can be seen that the mixed extract of the ratio of Cheonma + pangeun 2:1 is excellent in dose-dependent antitussive activity.

Through the examples, it was found that Panhae muscle extract was excellent in Jinhae and Geodam, and even when combined with Cheonma extract, which was superior in efficacy to Panram muscle extract, the Jinhae and Geodam effects were excellent. In addition, it was confirmed that the effect of Jinhae and Geodam was excellent at the 2:1 compounding ratio of Cheonma and Panram muscle, and that the mixed extract of the Cheonma and Panram muscle 2:1 compounding ratios increased the capacity to increase the antitussive and expectorant effects. there was. Therefore, it can be said that the mixed extract of the ratio of Cheonma and Panrameun 2:1 not only produces antitussive and expectorant effects, but also dose-dependently effects of antitussive and expectorant.

Claims (14)

Pharmaceutical composition for the prevention or treatment of antitussive expectorant comprising panrameun extract as an active ingredient.
According to claim 1,
The panram muscle extract is a pharmaceutical composition for the prevention or treatment of antitussive expectation, characterized in that the extract using a polar solvent.
According to claim 2,
The polar solvent is a pharmaceutical composition for preventing or treating antitussive expectation, characterized in that ethanol.
According to claim 2,
The extract is a pharmaceutical composition for preventing or treating antitussive expectora, characterized in that 50% ethanol is used.
According to claim 1,
The composition is a pharmaceutical composition for preventing or treating antitussive expectation, characterized in that it may further include a cheonma extract.
The method of claim 5,
In the case of a mixed extract of the cheonma extract and panram muscle extract,
1~3: Included in a weight ratio of 3~1
A pharmaceutical composition for preventing or treating Jinhae Gadam.
The method of claim 6,
In the case of a mixed extract containing the Cheonma extract and Panram muscle extract,
Cheonma extract: containing panram muscle extract in a weight ratio of 2:1
A pharmaceutical composition for preventing or treating Jinhae Gadam.
The method of claim 5,
In the composition
The mixed extract containing Cheonma extract and Panram muscle extract is characterized in that it is administered to a mammal at a dose of 100 to 800 mg/kg based on the composition.
A pharmaceutical composition for preventing or treating Jinhae Gadam.
Health functional food for prevention or improvement of Jinhae expectorant containing Panram muscle extract as an active ingredient.
The method of claim 9,
The pangeun extract is a health functional food for the prevention or improvement of Jinhae expectorant, characterized in that it may further include a cheonma extract.
The method of claim 10,
The food
When using a mixed extract containing the cheonma extract and panram muscle extract,
Cheonma extract: containing Panram root extract in a weight ratio of 1~3:1~3
Health functional food for prevention or improvement of Jinhae Gundam.
The method of claim 11,
In the case of a mixed extract containing the Cheonma extract and Panram muscle extract,
Cheonma extract: containing panram muscle extract in a weight ratio of 2:1
Health functional food for prevention or improvement of Jinhae Gundam.
The method of claim 10,
The extract is characterized in that the reflux extract using 50% ethanol
Health functional food for prevention or improvement of Jinhae Gundam.
The method of claim 9,
The health functional food,
Characterized in that at least one of powders, granules, tablets, capsules, syrups and beverages
Health functional food for prevention or improvement of Jinhae Gundam.

Images