KR20110045371A - Composition comprising extracts of Radix Smilax china, Paecilomyces japonica and Momordica charantia for hypoglycemic agent] - Google Patents

Abstract

PURPOSE: A composition containing herbal material extract for reducing blood glucose is provided to be efficiently used for treating hypertension. CONSTITUTION: A composition for reducing blood glucose contains extract of 5-30 weight% of Radix Smilax china, 30-60 weight% of Momordica charantia, and 30-60 weight% of Paecilomyces japonica as an active ingredient. The extract is isolated using organic solvent of C1-C4 or hot water. A health food contains the composition for reducing blood glucose. A pharmaceutical composition for preventing or treating diabetes contains the composition for reducing blood glucose.

Description

Composition containing extracts of Radix Smilax china, Paecilomyces japonica and Momordica charantia for hypoglycemic agent}

The present invention relates to a composition having an effect of lowering blood sugar by using the extract of the herbal medicine, and relates to a composition that can be used for the treatment or prevention of diabetes through the lowering of blood sugar.

Diabetes mellitus refers to a group of diseases in which glucose metabolism occurs due to insufficient insulin action. Diabetes is characterized by chronic hyperglycemia and involves metabolic abnormalities.

Characteristic symptoms of early diabetes include polyuria, polydipsia, polyphagia, and weight loss. Clinical symptoms include urinary glucosuria, hyperglycemia, and nodules. Abnormal glucose tolerance test, asthenia, etc. may be present.

Complications caused by diabetes are classified as complications due to acute metabolic disorders and chronic complications. Acute complications include hypoglycemia, diabetic ketoacidosis, hyperosmolar nonketotic coma, and chronic complications include diabetic neuropathy and diabetic retinopathy. diabetic retinopathy) and diabetic nephropathy.

Diabetes is divided into type 1 diabetes mellitus and type 2 diabetes depending on insulin-dependence.

Type 1 diabetes is a disease caused by absolute deficiency of insulin by autoimmune destruction of pancreatic β cells. Type 1 diabetes is often insulin-dependent diabetes mellitus (IDDM) because symptoms are greatly improved by the administration of insulin. Treatment of type 1 diabetes depends absolutely on the administration of insulin.

Type 2 diabetes is caused by decreased insulin secretion and insulin resistance. The type 2 diabetes mellitus can be divided into insulin secretion predominant diabetes and insulin resistance superior diabetes according to the degree of involvement of these two factors. There are many causes of type 2 diabetes, including environmental factors, heredity, obesity, stress, aging, frequent pregnancy, and modest drug abuse. Prevention and treatment of type 2 diabetes can be treated with moderate diet and exercise therapy in mild cases, with moderate oral medication or insulin therapy in severe cases.

Diabetes is a chronic disease that cannot be cured by current treatments. The most important point in preventing or treating diabetes is controlling blood sugar to a normal level.

Oral hypoglycemic agents currently used in the clinic can be classified as follows according to the mechanism of action.

Sulfonylurea drugs stimulate the secretion of insulin made by pancreatic β cells. The most common side effects of sulfonylureas are hypoglycemia, which can cause digestive problems, tachycardia, headaches, and hepatotoxicity.

Biguanide (metformin) drugs regulate blood sugar by inhibiting glucose production in the liver. Since biguanides do not cause hypoglycemia unlike sulfonylureas, they are used either alone or in combination with sulfonylureas when they fail to treat sulfonylureas. Although biguanides are rare, lactic acidosis can develop, which is one of the most serious complications in patients with kidney disease. Major side effects are gastrointestinal symptoms, which occur in 10% to 30% of patients and may cause anorexia, nausea, abdominal discomfort and diarrhea.

α-glucosidase inhibitor, also called acarbose, inhibits the action of alpha-glucosidase, a type of digestive enzyme present in the small intestine mucosa, which delays sugar absorption and degradation. Prevents rapid blood sugar rise. Major side effects include bloating and indigestion due to digestive problems.

Thiazolidinediones are drugs that improve insulin resistance by stimulating receptors in the body called PPAR-gamma receptors in tissues such as fatty liver and bone muscle. These insulin resistance improvers may appear to increase weight with increasing blood flow. In particular, hepatotoxicity has been reported due to the first-generation insulin resistance improver, troglitzione.

Meglitinide-based drugs are non-sulfonylurea-based and bind to sulfone urea receptors of pancreatic β-cells to induce insulin secretion, which is fast and effective in lowering blood glucose compared to sulfonylureas. However, mild hypoglycemia may be induced.

As described above, oral chemical hypoglycemic agents have a wide variety of mechanisms of action, and often have various side effects due to their properties as chemical agents.

Therefore, in recent years, interest in oriental medicine that prevents or treats diseases based on natural products has been steadily rising, and there have been many efforts to cure diabetes through folk remedies transmitted from ancient medicines or whales. .

Korean Patent Laid-Open No. 2002-0031911 discloses that red ginseng, thorny stem stem and Cordyceps sinensis extract are effective against type 2 diabetes.

Korean Patent Laid-Open No. 2002-0061902 discloses that bokbunja extract is effective for diabetes.

Korean Patent Publication No. 2005-0043092 discloses that the extract of Allium.victorialis L is effective against diabetes.

Korean Patent Laid-Open No. 2006-0056645 discloses a pharmaceutical composition for the prevention and treatment of diabetes mellitus, diabetic complications, insulin resistance and thereby insulin resistance syndrome comprising a gastric gland extract.

Korean Patent Laid-Open Publication No. 2005-0001254 discloses a blood glucose lowering composition containing silkworm cordyceps, yeast and magnesium.

Republic of Korea Patent Registration No. 10-0827350 discloses a pharmaceutical composition for lowering blood sugar comprising a composite herbal extract extracted with water using a mixed herbal medicine containing cheonhwabun, cheoncho, Sangbaekpi, Shiho and Sukjihwang as an active ingredient.

Republic of Korea Patent Publication No. 2007-0118754 is a herbal extract for the prevention or treatment of diabetes consisting of white ginseng, Astragalus, licorice, goji berry, mulberry leaf, brown root, haegocho, ogalpi, Schisandra chinensis, golden, medicinal herb, yellowjung, peony and dough It is starting.

As described above, in the Oriental medicine, hypoglycemic effect has been raised for hundreds of herbal medicines since ancient times, and it has been used as a folk remedy, but until now, specific and systematic research results are inadequate and mixed in various herbal medicines. The research on the synergistic effect of the composition of the composition is more incomplete.

The present inventors conducted a steady study of various herbal medicines known to have an effect on lowering blood sugar, and as a result, the composition extracted from Tobokyeong, Yeoju and Cordyceps sinensis was significantly significant compared to the composition extracted from alone or two. The present invention was completed by confirming that the drop effect appeared.

The present invention provides a composition for lowering blood sugar, which contains extracts of Tobok-ryeong, Yeoju and Cordyceps sinensis as active ingredients. The extract is preferably extracted from the medicinal herb consisting of 5 to 30% by weight, Cordyceps sinensis 30 to 60% by weight, Yeoju 30 to 60% by weight with respect to the total weight%, C ₁ ~ C _{4 with} an organic solvent or hot water It is preferable to extract.

In addition, the present invention provides a health functional food comprising a composition for lowering blood sugar, which contains extracts of Tobok-ryeong, Yeoju and Cordyceps sinensis as active ingredients. The dietary supplement may be taken orally in various forms such as powders, granules, tablets, capsules, pills, or beverages as necessary.

In another aspect, the present invention provides a pharmaceutical composition for preventing or treating diabetes, including a composition for lowering blood sugar, which comprises extracts of Tobok-ryeong, Yeoju and Cordyceps sinensis as active ingredients. The dietary supplement may be taken orally in various forms such as powders, granules, tablets, capsules, pills, or beverages as necessary.

The compositions extracted with Tobok-ryeong, Cordyceps sinensis and Yeoju of the present invention have a significantly significant hypoglycemic effect compared to the compositions extracted from these herbs alone or two. Therefore, the present invention may be effectively used for the management of hyperglycemia due to diabetes, and can be prepared as a dietary supplement, as well as pharmaceutical products, away from diabetes treatment currently relying on chemical oral hypoglycemic agents and insulin therapy. It can be usefully provided to the patient.

Hereinafter, the present invention will be described in detail.

Radix Smilax china is the root of Smilax china Linne, a deciduous shrub of the Liliaceae family, whose stem is about 3m long, and its stem is curved in the node. The thick, hard rhizome stretches sideways. In China or Japan, Tobok Ryong is also referred to as the root of the Smilax glabra Roxburg. Its origin is Korea, China, Japan, and Indonesia. It harvests root stems in spring and autumn, and it is used for medicinal purposes after drying in the sun. In Korean medicine, Tobok-ryeong is known to lower heat, remove moisture, and detoxify. It is used for bone node pain, syphilis, gonorrhea, digestive, mercury poisoning, spear, playing window, boil, etc. It is also used for the treatment of. In addition, some are known to be used as a diabetes treatment.

The name Cordyceps sinensis is related to the invasion of cordyceps fungi, forming fungal nuclei in the insect's body in winter, and sprouting like grass in summer. Spores and mycelium of Cordyceps fungus invade insects, fungi and seeds of plants, and use the substances in the host as nutrients to form endogenous nuclei and then form fruiting bodies. To date, more than 800 species of Cordyceps invades insects worldwide. It has recently been demonstrated that Cordyceps sinensis has a very powerful immune function-enhancing and anti-cancer activity, and various documents have reported the effect of hypoglycemic activity. Snowfly Cordyceps ( Paecilomyces japonica ) is a fungus of the genus Cordyceps fungus Cordyceps sinensis, also called moth flower cordyceps.

The balsam pear or Momordica charantia is a vine annual herb of the dicotyledonous gourd. Fruits are gourds, long oval, narrow at both ends, hump-like projections, yellow-red, irregularly split, and covered with red flesh. Young fruit and red paprika are mainly edible and seeds are mainly medicinal. Yeoju is known to prevent the rise of glucose by inhibiting the action of α-glucosidase, a type of digestive enzyme present in the small intestine mucosa (Ahmed, I .; Adeghate, E .; Cummings, E. Sharma, AK Singh, J., Beneficial effects and mechanism of action of Momordica charantia juice in the treatment of streptozotocin-induced diabetes mellitus in rat., Molecular and Cellular Biochemistry, Volume 261, Number 1, June 2004, pp. 63 -70 (8)). In addition, a mixture of Yeoju seeds and grifola frondosa fruiting bodies is known to inhibit the action of α-glucosidase (Matsuur H ,; Asakawa C ,; Kurimoto M ,; Mizutani J., Alpha-glucosidase inhibitor from the seeds of balsam pear ( Momordica charantia ) and the fruit bodies of Grifola frondosa.Biosci Biotechnol Biochem 2002; 66: 1576-8.).

The present inventors conducted a steady study of various herbal medicines known to have an effect on lowering blood sugar, and as a result, the composition extracted from Tobokyeong, Yeoju and Cordyceps sinensis was significantly significant compared to the same amount of the composition extracted from two or two. It was confirmed that the hypoglycemic effect appeared.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are illustrative, and the spirit of the present invention is not limited to the following examples, and may be modified and practiced according to the skill level of those skilled in the art.

Example 1 Composition extracted from Tobok Ryong

1.00 kg of finely ground Tobok-Ring was placed in a 10 L round bottom flask, followed by about 6 kg of 70% (v / v) ethanol and heated to reflux for 5 hours. The reaction solution was filtered directly using Whatmann filter paper in the hot state. The ethanol solution was removed from the filtration mother liquor using a rotary evaporator to finally obtain a brown composition. The yield was about 26% by weight based on the total weight of the raw materials.

Example 2 A composition extracted from Snow Cordyceps sinensis

1.00 kg of finely ground snow Cordyceps sinensis as a raw material was carried out in the same manner as in Example 1 to obtain a composition of about 29% by weight based on the total weight of the raw materials.

Example 3 Composition Extracted from Yew Lime

1.00 kg finely ground bitter gourd was used as the raw material in the same manner as in Example 1 to obtain a composition of about 29 wt% based on the total weight of the raw materials.

Example 4 Composition extracted from Tobok-ryeong and Snowflower Cordyceps sinensis

0.20 kg of finely ground Tobok-ryeong and 0.80 kg of Snow Cordyceps sinensis were carried out in the same manner as in Example 1 to obtain a composition of about 28 wt% based on the total weight of the raw materials.

Example 5 Composition extracted from Tobokyeong and Yeoju

0.20 kg finely pulverized Tobokyeong, 0.80 kg of bitter melon was carried out in the same manner as in Example 1 to obtain a composition of about 28% by weight based on the total weight of the raw materials.

Example 6 Composition Extracted from Snowflake Cordyceps and Yeoju

0.55 kg of finely ground snow Cordyceps sinensis and 0.45 kg of bitter melon were carried out in the same manner as in Example 1 to obtain a composition of about 28 wt% based on the total weight of the raw materials.

Example 7 Composition extracted from Tobok-Ryeong, Snowflower Cordyceps and Yeoju

0.10 kg of finely pulverized Tobokyeong, 0.50 kg of Snow Cordyceps sinensis, and 0.40 kg of Yeoju were placed in a 10 L round bottom flask, followed by about 6 kg of 70% (v / v) ethanol and heated to reflux for 5 hours. The reaction solution was filtered directly using Whatmann filter paper in the hot state. The ethanol solution was removed from the filtration mother liquor using a rotary evaporator to finally obtain a brown composition. The yield was about 28% by weight based on the total weight of the raw materials.

Experimental Example 1: Experimental Animal Experiment Set

50 rats (C57 BLKS / J-db / db) of 6-week-old type 2 diabetes (C57 BLKS / J-db / db) were subjected to temperature 21 ± 3 ° C, relative humidity 50 ± 5% and roughness 200-300 for 14 days. After adapting to the laboratory under Lux conditions, 40 healthy animals closest to the mean mean blood glucose value (161.9 mg / dL) were selected and used for the experiment (5 per group). During the acclimation period, tap water was freely consumed as drinking water, and experimental animal feed was freely consumed as feed. The composition of the experimental animal feed is shown in Table 1 below.

Composition of Feed for Experimental Animal turn nutrient Content rate (%) One Crude protein 22.1 or later 2 Low fat 3.5 or more 3 fibrin 5.0 or less 4 Ash 8.0 or less 5 calcium 0.6 or more 6 sign 1.2 or less

[Feed Maker: Superfeed Co., Ltd. (Wonju, Gangwon-do, Korea)]

After 40 animals selected as above were randomly divided into 8 groups of 5 animals, the control group was orally administered 10 mL / kg body weight of distilled water once daily for 4 weeks to diabetic mice.

In the first group, a sample of the composition obtained by extracting 1.00 kg of Tobokyeong was suspended in a 5% aqueous solution by weight and orally administered at 10 ml / kg of body weight once daily for 4 weeks.

In the second group, the composition sample obtained by extracting 1.00 kg of snowflake Cordyceps sinensis according to Example 2 was suspended in a 5% aqueous solution by weight ratio and orally administered once daily for 4 weeks at 10 ml / kg body weight.

In the third group, a sample of the composition obtained by extracting 1.00 kg of Yeoju in accordance with Example 3 was suspended in a 5% aqueous solution by weight ratio and orally administered once daily for 10 weeks at 10 ml / kg of body weight.

In the fourth group, the composition samples obtained by extracting 0.20 kg of Tobokyeong and 0.80 kg of Snow Cordyceps sinensis according to Example 4 were suspended in a 5% aqueous solution based on weight ratio and administered orally at 10 ml / kg of body weight once daily for 4 weeks.

According to Example 5, the composition samples obtained by extracting 0.20 kg of Tobokyeong and 0.80 kg of Yeoju were suspended in a 5% aqueous solution by weight, and orally administered at 10 ml / kg of body weight once daily for 4 weeks.

According to Example 6, the composition sample obtained by extracting 0.55 kg of snowhopper Cordyceps sinensis and 0.45 kg of bitter melon was suspended in a 5% aqueous solution by weight ratio and orally administered once daily for 4 weeks at 10 ml / kg body weight.

The seventh group was orally administered at 10 ml / weight kg once daily for four weeks by suspending the composition sample obtained by extracting the bokbokyeong 0.10 kg, snow Cordyceps sinensis 0.50 kg, Yeoju 0.40 kg in accordance with Example 7 in a 5% aqueous solution by weight ratio I was.

Experimental Example 2: Confirming the hypoglycemic effect of the hypoglycemic composition

Type 2 diabetes-induced mice (C57 BLKS / J-db / db) administered orally at 10 ml / kg body weight once daily by suspending the composition samples obtained in Examples 1 to 7 in a 5% aqueous solution by weight. Blood was collected from the tail at 2 and 4 weeks of administration from the tail and blood glucose was measured using a Superglucocard II blood glucose meter (Arkray Inc., USA).

Table 2 below compares the hypoglycemic effect according to each example with the control group.

Blood glucose reading (mean value for each group, rounded to two decimal places) (Unit: mg / ㎗) Herbal Medicine start date after 2 weeks 4 weeks later Final weight (g) Hypoglycemic effect Control group - 149.6 381.4 431.2 45.3 - First group  Tobok Spirit 153.2 324.0 290.4 42.6 32.7% 2nd group  Cordyceps sinensis 160.2 330.6 267.8 41.7 37.9% 3rd group  Yeoju 154.6 315.6 275.4 40.6 36.1% 4th group  Tobokyeong, Cordyceps sinensis 155.4 320.8 256.2 42.3 40.6% Fifth group  Tobokyeong, Yeoju 154.2 305.4 271.8 41.6 37.0% 6th group  Cordyceps, Yeoju 156.4 299.2 257.4 41.5 40.3% 7th group  Tobokyeong, Cordyceps, Yeoju 152.6 279.2 225.6 40.9 47.7%

As can be seen from the results shown in Table 2, the extract alone (groups 1, 2, 3) showed a hypoglycemic effect of about 32 to 38% compared to the control group, two extracts (four, 5, Group 6) had a hypoglycemic effect of about 37 to 41%, and the three hemoglobin extracts (Group 7) of the present invention had a 47.7% reduction compared to the control group, which was significantly more significant than the two extracts. It can be seen that there is a falling effect.

From these results, it was confirmed that synergistic effect of superior hypoglycemic activity was observed in the case of the mixed extract of Tobokyeong, Cordyceps sinensis, and Yeoju.

Therefore, the mixed extract composition of Tobok-ryeong, Cordyceps sinensis and Yeoju according to the present invention may be effectively used for the management of hyperglycemia due to diabetes. Of course, it can also be prepared as a medicine, it can be usefully provided to various diabetics.

Claims (6)

Tobokyeong, Yeoju and Cordyceps sinensis lower blood sugar composition comprising as an active ingredient. The composition of claim 1, wherein the extract is extracted from the Chinese herbal medicine consisting of 5-30% by weight, 30-60% by weight of Cordyceps sinensis, and 30-60% by weight of Yeoju relative to the total weight. The composition of claim 1 or 2, wherein the extract is extracted with C ₁ ~ C ₄ organic solvent or hot water. The composition of claim 1, wherein the cordyceps is snowflake cordyceps. Health functional food comprising the composition of claim 1 or 2. A pharmaceutical composition for preventing or treating diabetes, comprising the composition of claim 1.