KR20090126469A - The herb extract composition for the blood glucose reducing action - Google Patents

Abstract

PURPOSE: A medicinal herb composition for reducing blood sugar is provided to increase insulin secretion and prevent and treat diabetes. CONSTITUTION: A medicinal herb composition for reducing blood sugar comprises 850 weight parts of Momodica charantia Linn, 560-1,024 weight parts of Salicornia herbacea, 56-104 weight parts of Kalopanacis cortex, 35-65 weight parts of ginseng, 84-156 weight parts of Acanthopanax senticosus, 42-78 weight parts of Mori Radicis Cortex, 49-91 weight parts of lycii Fructus; 21-39 weight parts of Salviae Radix, 28-52 weight parts of Astragali Radix, and 105-195 weight parts of banaba. The medicinal herb extract is isolated using low alcohol of 1-4 carbon atoms, water or their mixture. A zinc gluconate, magnesium gluconate, and potassium cluconate are added to the extract as nutritional supplement.

Description

The Herb Extract Composition for the Blood Glucose Reducing Action}

The present invention relates to a herbal composition for lowering blood sugar using herbal medicine (medicinal herbs), and in detail, 10 kinds of herbal medicines (medicinal herbs) which are Yeoju, Hamcho, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Gojija, Dansam, Hwanggi, Banaba It relates to a blood sugar-lowering herbal composition comprising a blood glucose-lowering herbal composition of the present invention as a composition having the effect of preventing and treating diabetes by lowering blood sugar and increasing insulin secretion, insulin-dependent diabetes mellitus (type 1 diabetes) And insulin non-dependent diabetes mellitus (type 2 diabetes mellitus), which is an excellent invention that exhibits an excellent therapeutic effect, and in particular, the state of drug expression between individuals is stabilized and the uniformity of therapeutic effects is stabilized.

Diabetes and its complications are high mortality rates worldwide, along with cancer and renal circulatory disease. Mortality continues to increase, and the onset of the disease has gradually occurred in childhood. Patients have a high risk of developing complications such as eye disease, kidney disease and heart disease.

Symptoms of diabetes vary, but the three major symptoms are Daum, multi urine, Dasik. In other words, due to the high blood sugar level in the blood, glucose and a large amount of water are excreted by osmotic pressure, and the urine is frequently seen. Therefore, as a result of dehydration, a person is severely thirsty. As a result, a lot of weight is reduced.

Diabetes is largely classified into insulin dependent diabetes mellitus (type 1) and insulin independent diabetes mellitus (type 2).

Insulin-dependent, type 1, can also cause serious symptoms, such as diabetic ketoacidosis. In the United States and Europe, about 5 to 10% of all diabetics are estimated to be insulin-dependent diabetic patients with type 1, but in Korea, about 1 to 2%.

It is associated with HLA antigens (HLA, DR, DQ), a gene that causes type 1 insulin-dependent diabetes. It has been found that this autoimmune mechanism is involved in the invention of diabetes mellitus and autoantibodies to islet cell insulin are produced. In recent years, efforts have been made to detect such autoantibodies before type 1 diabetes occurs to slow or prevent the onset of diabetes.

Insulin-dependent diabetes mellitus, type 2, develops mainly after the forties and accounts for most of the diabetic patients in Korea. Unlike type 1, it is called adult-type diabetes and its cause is not clear yet, but it is known to be caused by genetic factors and environmental factors involved.

Genetic factors include a variety of familial concentrations and nearly 90 to 100% disease matching among identical twins, 58% for children when both parents are diabetic, and 27% for parents who are all diabetic. When it is healthy, it is known that it occurs about 0.9%. Environmental factors, such as high calorie intake, lack of exercise, obesity, stress and substance abuse, which are rapidly increasing with the recent economic growth, are causing the diabetes.

The pathogenesis of diabetes mellitus is observed in both pancreatic beta cells and in insulin secretion and in target insulins (insulin resistance). It is not clear what changes are of primary importance.

In the treatment of diabetes, hypoglycemic agents include sulfonylureas, biguanides, and acarbose. Among them, sulfonyl-urea preparations are second generation products with short duration of action and strong hypoglycemic effects. It is mainly used, and in patients with obese adult diabetes, biguanides and acarbose are used for diabetics with normal weight or more.

In addition, insulin used in the form of injection is excellent in hypoglycemic effect, but it is inconvenient to use, and low blood sugar causes complications.

In addition to the drug therapy, there are 158 types of folk remedies in Korea. There are 153 types of folk remedies, and about 73% of adult diabetics have used folk remedies more than once.

The study of diabetes treatment drugs is focused on research and treatment fields. In the case of insulin-dependent cases, studies for preventing pancreatitis and treating diabetes are mainly conducted on disease model animals such as MOD mice and most diabetes treatment strategies. Focuses on suppressing and regulating immune responses to prevent beta cell destruction. Insulin-dependent diabetes mellitus (type 1 diabetes), an autoimmune disease, is due to augmented abnormal immune function and various immunotherapies have been attempted to prevent beta cell destruction of the pancreas.

Neonatal Thymectomy can eliminate T cells to suppress the development of diabetes in NOD mice. In addition, it has been found that the treatment of antibodies to T cells removes T cells or macrophages to inhibit the onset of diabetes. It has been reported that diabetes can be suppressed by the production and action of free radicals such as NO secreted from immune cells and antioxidants, such as nicotinamide, vitamin E, and probucol. Antioxidants have been reported.

Therapies using immunosuppressants are being studied. However, the treatment of diabetes with Glucocorticoid or Cyclophosphamide has not shown any significant effect. Recently, the possibility of treatment of diabetes with cyclosporin A and rapamycin has been suggested. The result is not yet clear.

Treatment with immunosuppressive agents causes secondary infection, kidney toxicity, liver toxicity due to excessive immune suppression, which still has many problems for use as an antidiabetic agent, and sometimes causes cancer during long-term treatment. .

Recently, studies on the prevention and treatment of diabetes mellitus by immunomodulators other than immunosuppressants have been conducted, and it has been reported that the cytokines such as IL-4 and IL-10 inhibit the diabetes of NOD mice. In addition, efforts are being made to develop a therapeutic agent that can lower insulin side effects and treat insulin dependent and non-dependent diabetes at the same time. Folk remedies have traditionally been known to have a variety of therapeutic effects with low toxicity. To date, the effects of diabetes treatment using dozens of herbal medicines have been reported, and there are reports that insulin-dependent and non-dependent diabetes can be treated simultaneously.

In this regard, the present inventors conducted intensive studies to develop a composition capable of treating insulin-dependent and non-dependent diabetes mellitus by combining herbal medicines (medicinal herbs) with few side effects. Antihyperglycemic herbal composition containing herbal extracts of haejupi, haejupipi, sanggipi, baekryepi, gojija, ginseng, red ginseng, astragalus and banaba can promote insulin secretion of beta-cells and treat insulin-dependent (type 1) diabetes After conducting human experiments that it is possible to treat insulin-independent (type 2) diabetes by suppressing rapid blood glucose rise and removing insulin resistance of peripheral tissues, it was found that the blood sugar lowering effect and the stability of individual treatment effects were excellent. The present invention has been completed.

Accordingly, an object of the present invention is to provide a hypoglycemic herbal composition containing Yeoju, Hamcho, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Wolfberry, Dansam, Astragalus and Banaba herbal extracts as active ingredients.

The present invention relates to a herbal composition for lowering blood sugar using herbal medicine (medicinal herbs), and in detail, 10 kinds of herbal medicines (medicinal herbs) which are Yeoju, Hamcho, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Gojija, Dansam, Astragalus and Banaba It relates to a blood sugar-lowering herbal composition containing a blood glucose-lowering herbal composition of the present invention as a composition having an effect of preventing and treating diabetes by lowering blood sugar and increasing insulin secretion, insulin-dependent diabetes mellitus (type 1 diabetes) and It is an excellent invention that shows excellent therapeutic effect on insulin non-dependent diabetes mellitus (type 2 diabetes mellitus), and in particular, the state of drug expression among individuals is stabilized and the equality of therapeutic effects is stabilized.

Hereinafter, the present invention will be described in more detail.

The extract is a pulverized pulverized herbal medicine (medicinal herbs) material, the Chinese medicine (medicinal herbs) material is added to the solvent and heated, and then filtered and the extracted liquid extract or herbal medicine (medicinal herbs) material is added to the solvent and heated and filtered After the rough, the extract is characterized in that it is composed of a powder extract obtained by drying under reduced pressure. The composition of the present invention includes a mixed powder extract including a single extract of each medicinal herb or a complex liquid extract prepared by extracting together two or more of each herbal (medicinal herb) material.

Extracts according to the present invention typically include water extracts, solvent extracts using alcohol, extracts using a solvent mixed with water and alcohol. The method for producing an extract according to the present invention is known to those skilled in the art. All extraction methods can be applied, but are not limited thereto, such as an extraction method using a bath or room temperature. And the liquid extract extracted by the above method can be obtained by using the freeze-drying method or reduced pressure heating drying method of the herbal powder of each herbal (medicinal herb) material.

The herbal composition for lowering blood sugar of the present invention is 560 to 1,024 grams by weight of seaweed, 56 to 104 grams by weight of thawed skin, 35 to 65 grams by weight of ginseng, and 35 to 65 grams by weight of ginseng, 84 to 156 grams by weight, based on the dry matter. And extracts of herbal ingredients consisting of 42 to 78 grams of lettuce, 49 to 91 grams of wolfberry, 21 to 39 grams of salvia, 28 to 52 grams of Astragalus, and 105 to 195 grams of Banaba.

The above connotation includes not only the fruit but also the seed.

Lime is a vine plant of gourds. Its official name is Mormordica Charantia, which contains 120 milligrams of vitamin C. It contains 80 milligrams of strawberries, 90 milligrams of lemon, and 40 milligrams of cabbage. It contains a large amount. Vitamin C, which is contained in bitter melon, is hardly destroyed by heating the fruit. Yeoju also contains minerals such as beta-carotene, potassium, and iron, which are converted into vitamin A in the body, but a particularly important component of Yeoju is vegetable insulin (P-Insulin), which helps to burn glucose and prevent glucose from resynthesizing in the body. ) And the fat-soluble carantin (Charantin) that activates the pancreas to secrete insulin. Plant insulin has a similar effect to insulin in the body. It is a kind of peptide and is contained in fruits and seeds. In addition, Yeoju has many effects on improving insulin resistance. Insulin resistance refers to a condition in which insulin is weakly secreted but its action is poor, and sugar in the blood does not flow into the cells. Yeoju is also rich in antioxidants such as beta-carotene, which can also be used to prevent retinopathy and nephropathy (vascular diseases such as myocardial infarction and stroke).

The seaweed is a yearly grass of the dicotyledonous plant, the main plant, which grows on the beach. Stems are voracious, prominent, split 1-2 times, eggplants are fleshy, fleshy, hypertrophy, dark green, and turn red. The main ingredients in the seaweed contain 670 mg of calcium, 70 mg of iodine, 6.5 mg of sodium, 16 g of salt, 16 g of salt, and about 50% of linolenic acid, an unsaturated fatty acid. It contains high amounts of essential amino acids such as leucine, isoleucine, threonine, phenoalanine, methionine, lysine and histidine. It also contains a small amount of essential minerals such as chromium and zinc. In particular, chromium prevents hardening of the arteries, keeps fat in the blood normal, and metabolizes sugar with insulin. Zinc also helps to form insulin in the pancreas.

Haedongpi refers to the bark of the oak tree and its main components are Saponin, Flavonoids, Lignans, Phenols, Alkaloids, and amino acids. The oak tree is mainly divided into Korean, Japanese, and Chinese. Leaves are regenerated, palm-shaped, split into 5-9 branches, heart-shaped, lobe is ovate with pointed ends and sharp sawtooth. Haedongpi has a therapeutic and tonic effect of diabetes and strengthening the function of the kidney.

Morus bark is a mulberry root bark with a peculiar smell, sweet taste and cold nature. It treats seawater and asthma caused by waste heat and has a diuretic effect. Comprehensive pharmacological action is Jinhae, diuresis, lowering blood pressure, lowering blood sugar, soothing, analgesic, fever, jingyeong, antibacterial effect, especially the epidermis contains a lot of guwanon component that lowers blood pressure acts on vascular endothelial cells By inducing vasodilation, the effect of reducing cholesterol can be expected and the effect of lowering blood sugar levels can be expected.

Ingredients of the wolfberry include N-methylcalystegine B2, Linoleic acid, (-)-1,2-dehydro-α-cyperone, (+), as well as amino acids rich in betaine, rutin, sereburoside, carotenoids, and pinol derivatives. -α-cyperone Rich in vitamin B1, dehydroactinidiolide, safranal, β-ionone, megastimatrienone, 3-hydroxy-β-ionone and scopoletin. Inorganic ingredients include potassium, magnesium, calcium, iron and zinc. Betaine oxidizes choline to betaine aldehyde, which in turn oxidizes to betaine, a source of methyl groups with important physiological functions. Goji betaine is effective in promoting the function of the liver and stomach, atherosclerosis and hypertension, musculature and anemia. Wolfberry contains at least 0.5% betaine. Rutin was first isolated from buckwheat and contains high amounts. Rutin's main effect is that it has a strong effect on lowering blood sugar and cholesterol, which is good for preventing geriatric diseases, and flavonoid compounds similar to rutin have strong lipid metabolism control and antioxidant activity. Gojija water extract lowers blood pressure, has a temperature-lowering and antipyretic effect, and has an excellent effect of inhibiting the absorption of cholesterol, thereby preventing adult diseases caused by cholesterol and high fat. In addition, gojija contains a lot of vegetable unsaturated fatty acids linolenic acid and linoleic acid phosphorus and organic acids that can be converted by enzymatic reaction during the body's energy metabolism process promotes metabolism and is effective in preventing aging.

Prickly Pear is a dried bark of the Prickly Pear, a deciduous shrub belonging to the Ogalpiaceae. Its main components are 7 types of glycosides of triterpenoids, ereutererosides A, B, CD, E, F, and G. Other sugars such as glucose and galactose, carotene of polysaccharides, vitamins B1, B2, C, and minerals It contains abundantly. It also contains glycosides such as sterols, coumarins, ligunin, flavones, and futarein. Sterols have the effect of stimulating sex hormones, and coumarin has a pronounced sedative effect. Flavones work to expand coronary arteries, increase blood volume and increase the ability of the myocardium to withstand oxygen deficiency. Many of these glycosides stimulate the pituitary gland to increase the ability of various organs and to stimulate hormone secretion. In particular, eleuteroside B1, sesamin and isoproxidine have an effect on vasodilation, blood pressure stabilization, diabetic blood sugar level and prevention of diabetic complications.

Astragalus is a perennial herb belonging to the legumes. Its main ingredients include pomononetin, betaine, choline, and isocol tikenin, and its amino acids include GABA, which acts to strengthen blood pressure, isoflavonoids, and tonic. Contains saponin ingredients that work. Astragalus has a pharmacological effect that improves blood circulation by diuretic, cardiovascular, tonic blood, and blood vessel dilation.

Salvia is one of China's famous herb species belonging to the Chinese family, and its main ingredients are chlorogeninic acid, cinnaline, luteolin, caffeoyl quinic acid, cinnaropicrine, aguerrin, grosemine, and sirasoloside. It has a mechanism that promotes bile secretion activity and hepatocyte protection according to strong antioxidant activity. Pharmacological actions include glycemic control, liver function protection, antioxidant activity, fat absorption inhibition, coronary artery disease prevention effect.

The main ingredient of ginseng is 30 kinds of saponins, named ginsenosides, which are divided into triols, diols, and olannanes. Saponins present in ginseng include MG-Rb1, MG-Rc, MG-Rb1, MG-Rd. The pharmacological action of ginseng has the effect of inhibiting the degradation of insulin in the prevention and treatment of diabetes, anti-fat decomposition, and improving the symptoms associated with diabetes. Triol-based saponins in ginseng delay the onset of type 1 diabetes, reduce the incidence, increase insulin content in the pancreas and blood, and inhibit the infiltration of Iymphocytes into Pancreas. In particular, ginsenoside Rb2 inhibits urinary albumin excretion, suppresses kidney weight inhibition and hypertrophy. In addition, the pharmacological action of ginseng has several effects, including blood pressure control, arteriosclerosis, and hyperlipidemia.

The main components of banaba are composed of cholosorbic acid, zinc, iron, calcium, etc. The banana leachate, cholosorbic acid, has been shown to significantly lower blood sugar levels in the blood, inhibit the rise in blood sugar levels, and have no effect on hypoglycemia. .

Extracts of the herbal ingredients (medicinal herbs), Yeoju, Hamcho, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Gojija, Dansam, Hangi, Banaba, each having 1 to 4 carbon atoms of low alcohol, water or mixed solvents thereof Yeoju powder extract, seaweed powder extract, thaw skin powder extract, ginseng powder extract, prickly pear skin powder extract, lettuce powder powder extract, wolfberry powder extract, red ginseng powder extract, Astragalus powder obtained by decompression, heat drying extraction or freeze drying after filtration Extracts and Banaba powder extracts can be obtained. Each powder extract obtained is characterized in that it has a water content of 8% or less.

The extract of Yeoju was put in a solvent corresponding to 8 to 10 times the weight of Yeo fruit and seeds, which were mixed with water and ethanol in a ratio of 3: 7 and filtered after heating and extracting at 70 to 95 ° C. for 5 to 8 hours. Obtained by drying under reduced pressure at 60 to 80 ° C;

The seaweed extract was added to the solvent corresponding to 8 to 10 times the weight of the seaweed blended with water and ethanol in a ratio of 3: 7, and the mixture was filtered by heating and heating at 70 to 95 ° C for 5 to 8 hours, and then decompressed at 60 to 80 ° C. The mixture was heated to obtain a seaweed powder, and the obtained powder was heated at a temperature of 450 to 500 ° C. for about 45 to 50 hours, dissolved in water corresponding to a weight ratio of 2 to 3 times the weight of the powder obtained, filtered, and then filtered to 2.5 times of the obtained liquid. Mixed with the corresponding ethanol, filtered by heating for 2 to 4 hours at 70 ° C., followed by filtration and drying under reduced pressure at 60 to 80 ° C .;

The thawed skin extract was added to a solvent corresponding to 8 to 10 times the weight of thawed skin in the ratio of water and ethanol in a ratio of 3: 7 and filtered by heating and extracting at 70 to 95 ° C for 5 to 8 hours, followed by decompression at 60 to 80 ° C. Obtained by heating to dryness;

Ginseng extract is added to the solvent corresponding to 8 to 10 times the weight of ginseng blended in water and ethanol 3: 7 ratio, and extracted by heating for 5 to 8 hours at 70 to 95 ℃ filtered under reduced pressure at 60 to 80 ℃ Obtained by heating to dryness;

Prickly Pear Extract Extracts Prickly Pear Extract into water and ethanol in a solvent corresponding to 8 to 10 times the weight part of Prickly Pear skin at a ratio of 3: 7, filtered by heating and extracting at 70 to 95 ° C. for 5 to 8 hours, and drying under reduced pressure at 60 to 80 ° C. Obtained by;

Extract of lettuce extract is added to the solvent corresponding to 8 to 10 times the weight part of the lettuce extract mixed with water and ethanol 3: 7 ratio, and extracted by heating for 5 to 8 hours at 70 to 95 ℃ filtered under reduced pressure at 60 to 80 ℃ Obtained by heating to dryness; Goji extract is added to the solvent corresponding to 8 to 10 times the weight of the weight of wolfberry mixed with water and ethanol 3: 7 ratio, and extracted by heating for 5 to 8 hours at 70 to 95 ℃ filtered under reduced pressure at 60 to 80 ℃ Obtained by heating to dryness;

Salvia extract is added to the solvent corresponding to 8 to 10 times the weight of the salvia mixed with water and ethanol in a ratio of 3: 7, and filtered by heating and extracting for 5 to 8 hours at 70 to 95 ℃ and reduced pressure at 60 to 80 ℃ Obtained by heating to dryness;

Astragalus extract is poured into a solvent corresponding to 8 to 10 times the weight part of the sulfur group, which is blended with water and ethanol 3: 7 ratio, and extracted by heating for 5 to 8 hours at 70 to 95 ℃ filtered under reduced pressure at 60 to 80 ℃ Obtained by heating to dryness;

Banaba extract was poured into a solvent corresponding to 8 to 10 times the weight of banaba blended with water and ethanol in a ratio of 3: 7 and filtered by heating and extracting at 70 to 95 ° C. for 5 to 8 hours, followed by filtration at 60 to 80 ° C. Obtained by heating to dryness.

In addition, during the powder obtaining process of the extract of the herbal ingredient, the liquid extract containing the herbal ingredient in an aqueous solution in which the ethanol component is completely removed by volatilizing the ethanol and water at 70-90 ° C. before drying under reduced pressure may be used instead of the powdery extract. have.

If liquid extracts are used, they are packaged and sterilized. As the sterilization process, a method of heat treatment at 80 to 90 ° C. for 30 minutes to 1 hour at 100 to 110 ° C. for 30 minutes to 1 hour using a hot water bath or an auto claver is used.

Yeoju powder extract, seaweed powder extract, thaw skin powder extract, ginseng powder extract, prickly pear skin powder extract, lettuce powder extract, goji berry powder extract, salvia powder extract, Astragalus powder extract, which are contained as an active ingredient in the herbal composition for lowering blood sugar of the present invention The Banaba powder extract herbal composition is packed and packed after mixing.

In addition, Yeoju powder extract, seaweed powder extract, Haedongpi powder extract, ginseng powder extract, prickly pear skin extract, lettuce extract powder, wolfberry powder extract, sweet ginseng powder extract, Astragalus powder extract, banaba powder extract herbal composition, after mixing the appropriate amount of solvent Can be mixed and packaged in a vinyl polyethylene pack or plastic polyethylene container.

The herbal composition for lowering blood sugar of the present invention has a function of lowering blood sugar, preventing a rise in blood sugar, and increasing insulin secretion, insulin-dependent (type 1 diabetes), and insulin-independent (type 2 diabetes) It has an excellent therapeutic effect, and the effect of stabilizing the expression of drugs among the individuals shows the effect of equalizing the treatment effect.It helps to control blood glucose and prevent diabetes of gradually increasing diabetic patients. It helps.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the scope of the present invention is not limited thereto. It will be appreciated that modifications may be made to methods commonly known in the art and that such modifications are also within the scope of the present invention.

Example 1 Preparation of a Food Composition for Lowering Blood Sugar

On a dry basis, 850 g of bitter gourd and seeds are added to 2,040 ml of water, 4,760 ml of a 95% ethanol solution is heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 68 g of the lychee powder extract.

After cutting 800g of the vinegar on a dry basis, 4,480 ml of 95% ethanol solution was added to 1,920 ml of water based on the weight of the vinegar and heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure and heated at 60 to 70 ° C. to obtain a glasswort powder, and the obtained powder was heated at a temperature of 450 to 500 ° C. for about 45 to 50 hours, and then the weight ratio of the obtained powder was 2-3 times. It is dissolved in water, filtered, and then mixed with an ethanol solvent corresponding to 2.5 times of the obtained liquid and heated at 70 ° C. for 2 hours. The heated extract was filtered and dried under reduced pressure at 60-80 ° C. to obtain 72 g of the extract.

On a dry basis, 80 g of thawed skin is added to 192 ml of water, 448 ml of a 95% ethanol solution is heated at 85 ° C. for 8 hours. The extract obtained by filtering the heated extract was dried under reduced pressure at 60 to 70 ° C. to obtain 7.6 g of thawed skin powder extract.

On a dry powder basis, 50 g of ginseng is added to 120 ml of water and 280 ml of a 95% ethanol solution and heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 6 g of ginseng powder extract.

On a dry basis, 120 g of thorns are added to 288 ml of water, 672 ml of 95% ethanol solution is heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 7.7 g of Prickly Pear Extract.

On the basis of the dry matter, 60 g of baekrye skin is added to 144 ml of water and 336 ml of 95% ethanol solution and heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 7.2 g of baekpipi powder extract.

On a dry basis, 70 g of wolfberry is added to 168 ml of water, 392 ml of a 95% ethanol solution, and heated at 85 ° C. for 8 hours. The extract obtained by filtration of the heated extract was dried under reduced pressure at 60 to 70 ° C. to obtain 7.7 g of Goji berry powder extract.

On a dry basis, 30 g of salvia is added to 168 ml of 95% ethanol solution to 72 ml of water, and then heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 7.5 g of Salvia extract.

40 g of Astragalus on a dry basis is added with 224 ml of 95% ethanol solution to 96 ml of water and heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure and heated at 60 to 70 ° C. to obtain 8.8 g of the yellow powder powder extract.

On a dry basis, 150 g of Banaba is added to 360 ml of water, 840 ml of a 95% ethanol solution is heated at 85 ° C. for 8 hours. After filtering the heated extract, the obtained extract was dried under reduced pressure at 60 to 70 ° C. to obtain 12 g of a banana powder extract.

Yeoju powder extract obtained above; Seaweed powder extract; Thawed skin powder extract; Ginseng powder extract; Prickly Pear Powder Extract; Lettuce powder extract; Wolfberry powder extract; Sweet Ginseng Powder Extract; Astragalus powder extract; The Banaba powder extract was mixed and dried under reduced pressure and heat at 60 ° C. to obtain a water content of 8% or less, and then mixed homogeneously with a mixer to obtain 204.5 g of a powder extract of a total of ten mixed herbal ingredients.

The powder extract mixed with the total ten herbal ingredients obtained was packed into No. 0 capsules at 0.4 g weight and formulated into capsules.

[Diabetes Efficacy Test]

In order to examine the hypoglycemic effect of the herbal composition for hypoglycemic according to the present invention, an animal experiment was first conducted using a diabetic model mouse, and then the experimental effects of hypoglycemic effect on the human body were demonstrated.

As an animal experiment to investigate the hypoglycemic effect of the herbal composition for hypoglycemic according to the present invention, an ICR mouse was subjected to an Oral Glucose Tolerance Test (OGTT), and to type 2 (non-insulin dependent) diabetes. The effects on KK-AY mice were measured to determine the therapeutic effect of the treatment, and streptozotocin-induced diabetic and non-obese diabetic (NOD) mice were used to investigate the effects on type 1 (insulin-dependent) diabetes. Experiment.

The hypoglycemic effect on the human body was measured by improving the hypoglycemic effect and insulin secretion in insulin-independent diabetic patients, and the hypoglycemic effect on insulin-dependent diabetic patients.

Experimental Example 1 Measurement of Blood Glucose Dropping Level-Animal Experiment

In order to investigate the hypoglycemic effect of the hypoglycemic herbal composition according to the present invention, a sugar loading test (OGTT) was conducted using 5-6 week old ICR mice in SPF (Specific Pathogeus Free). The blood sugar lowering effect was compared between the herbal composition for lowering blood sugar of Example 1 and the control group.

As a test method, a total of 20 experimental animals were set up and divided into two groups of 10 animals. Experimental group 1 dissolved the composition of Example 1 in 0.85% saline solution, prepared by suspension by ultrasonic treatment, and orally administered daily by 0.1ml. In the control group 1, 0.1 ml of 0.85% saline was administered orally.

The dose was calculated based on the weight of 60kg adult weight was calculated to 13.4mg / kg, MOUSE DOSE was carried out by determining its 10 times 134mg / kg.

As a measuring method, the sample was orally administered to ICR mice fasted for 24 hours, and then 1 hour after administration, 2 g / kg of D-(+)-Glucose was administered 0.1 ml per 10 g body weight. The blood glucose measurement was performed using blood glucose meters manufactured by Johnson & Johnson, and blood was collected from the eye every 0, 10, 30, 60, 120 minutes, and the measurement results are shown in Table 1 and FIG. 1.

Table 1 below shows the results of the mean and standard deviation of blood glucose change after 10 minutes, 30 minutes, 60 minutes, and 120 minutes based on blood glucose at the initial start time by measuring the change in blood glucose for 120 minutes after oral administration of the sample. It is displayed.

TABLE 1

Experimental Example 2 Blood glucose lowering effect experiment in KK-Ay mice

KK-Ay mice are animals that exhibit type 2 diabetes insulin resistance from wild mice and blood glucose levels of 300-400 mg / dl. In order to confirm the therapeutic effect of the blood glucose lowering herbal composition of the present invention on KK-Ay mice, glucose loading test (OGTT) was performed using KK-Ay mice to compare the blood glucose effects. As an experimental method, 20 KK-Ay mice were divided into two groups of 10 (experimental group 2 and control group 2), and in experimental group 2, the herbal composition for lowering blood glucose of Example 1 was dissolved in 0.85% saline and 267 mg / 10ml / kg. Intraperitoneally administered daily at the dose of, control group 2 was intraperitoneally administered at a dose of 10ml / kg 0.85% saline each day.

Blood glucose was measured by taking blood from an orbital vein gun for 8 weeks after sample administration [FIG. 2], and the weight of KK-Ay mice was measured during the administration of the sample [FIG. 3]. Side effects were observed.

After the drug administration, KK-Ay mice were fasted for 24 hours, and 0.85% saline was used in the control group 2 mice, and the experimental group 2 mice were intraperitoneally administered at the dose of 267 mg / ml / kg of the herbal composition for lowering blood glucose of Example 1 Administered. After 90 minutes glucose was administered orally to all mice at a concentration of 1 g / kg. After 30, 60, 90 and 120 minutes, blood was collected from the orbital vein of the mouse and blood glucose was measured.

As shown in FIG. 2, the blood glucose level of the control group 2 mice started to increase after 2 weeks, and the 6th week showed the average hyperglycemia of 365 mg / dl or more, and the 8th week averaged 476 mg / dl. Blood glucose levels are shown. Mice in Experimental Group 2 showed little change in blood glucose with elapsed time, and showed a mean blood sugar of 296 mg / dl at 8 weeks [FIG. 2]. In addition, in the case of the control group 2, blood glucose increased more than 200 mg / dl after 30 minutes of glucose administration and then gradually decreased, whereas in the experimental group 2, the blood sugar increased about 100 mg / dl at the same time after glucose administration and then rapidly decreased. It showed an aspect.

In addition, the body weight of KK-Ay mice was measured for 8 weeks during the administration of the sample, and the side effects of the sample were observed. As a result, mice in the experimental group 2 did not show weight loss due to drug administration [FIG. 3].

Experimental Example 3 Experiments on Streptozotocin-induced Diabetic Mice

Streptozotocin (STZ) is an N-nitroso derivative of D-glucosamine that was originally developed as an antibiotic. Streptozotocin reduces intracellular NAD, which is known to destroy beta cells and cause diabetes. In this experiment, mice were repeatedly induced with 60 mg / kg of streptozotocin for 5 days to artificially induce type 1 diabetes, and then compared the blood glucose change by administering a sample to compare the pattern of type 1 (insulin-dependent) diabetes. The therapeutic effect of the herbal composition for lowering blood sugar of the invention was observed.

The experimental animals were supplied from the Korean Experimental Animal Center and managed in a clean room under the conditions of temperature 22 ± 3 ° C., humidity 55 ± 5%, and illumination 12L / 12D. The test animals were divided into four groups.

Control group 3: group not administered streptozotocin

Control group 4: group intraperitoneally administered streptozotocin 60 mg / kg 3 to 5 times

Control group 5: streptozotocin 60 mg / kg and placebo administered with 0.85% saline solution 10 ml / Kg

Experimental Group 3: Groups administered with streptozotocin 60mg / kg and placebo 10ml / Kg 0.85% saline solution and 267mg / ml / kg herbal composition for hypoglycemic use of Example 1

Drug administration was administered for 20 days and blood glucose and body weight were measured every 4 days.

The blood glucose measurement results of each of the control group 3, control group 4, control group 5 and experiment 3 2 are shown in Table 2 below, measured only to the highest blood concentration 500mg / dl in the measurement technology. The average blood sugar level of each group on the 8th day of drug administration was plotted in FIG. 4, and the change in the average blood sugar level of each group for 8 days before and after drug administration was shown in a graph of FIG. 5.

As shown in Fig. 4 and 5, when the blood glucose value of 300 mg / dl or more was determined as a diabetic mouse, 10 out of 10 mice tested in the control group 4, 10 out of 10 mice in the control group 5 was determined to be diabetic. In comparison, in experimental group 3 treated, only 1 out of 10 cases developed.

Through the above experiments, it was found that the incidence of diabetes mellitus of STZ mice was suppressed by administering the blood glucose lowering herbal composition of the present invention in the experimental group 3 to which the sample was administered.

[Table 2] Blood glucose measurement by individual of STZ mouse

Experimental Example 4 Clinical Experiment of Type 2 (Insulin-Independent) Diabetic Patients

Oral glucose load test (Oral Glucose Tolerance Test) was performed on 23 NIDD patients to verify the hypoglycemic effect of the hypoglycemic herbal composition of the present invention in insulin-independent (NIDD) diabetes patients. From July 2007 to January 2008, the laboratory consisted of 23 patients who were treated continuously for more than 60 days of non-insulin dependent patients who were treated at the ILDO Oriental Medical Center in Portland, Oregon, USA. The experimental drug used the hypoglycemic herbal composition according to the present invention.

First, the experimenter selection for the experiment was largely divided into three.

Selected test results of venous blood glucose ① ① Fasting blood glucose is more than 7.8mmol / L (140mg / dl) or fasting blood sugar is more than 11.1mmol / L (200mg / dl), ② Fasting blood sugar is 7.8mmol / L ( 140 mg / dl) or less glucose glucose 11.1mmol / L (200mg / dl) or more after the oral glucose load test ③ OGTT 1 hour after blood glucose and 2 hours after the blood sugar of 11.1mmol / L (200mg / dl) or more were selected However, the following cases were excluded from those who meet the above selection criteria.

Even if the blood glucose level before the test drug is met, the fasting blood sugar level is 7.8 mmol / L (140 mg / dl) or less after exercise or diet, or the blood sugar level is 11.1 mmol / L (200 mg / dl) or less after 2 hours. Those who violate the rules of the experiment and have an impact on outcomes, have been identified as Type 1 (insulin dependent) patients, have been diagnosed as having diabetic ketosis or asysis within one month of the experiment, have serious complications, or have mental illness. , Discontinued persons, 15 years of age or younger, or pregnant women

<Observation method>

After the blood glucose was measured 2 hours after an empty stomach and a meal, the Oral Glucose Tolerance Test, the Insulin Release Test, and the blood Amino-fructose were measured.

[Measuring glucose level after 2 hours on an empty stomach and a meal] of the above measurement is an essential item, and it measures every other item as needed. It was then compared with the pre-experiment measurements 60 days after dosing.

<Dose Method>

2.0 g of the test drug three times a day and once orally with a drink such as water 30 minutes before meals.

Among 23 insulin-independent diabetic patients, fasting blood glucose was 7.2mmol / L or less (130mg / dl) after dosing or 8.3mmol / L (150mg / dl) after 2 hours after meal. 20 patients (87.0%) or less, and 2 patients with oral fasting blood glucose of 8.3 mmol / L (150 mg / dl) or less than 10 mmol / L (180 mg / dl) after 2 hours of eating (9.7%) corresponded. In only one case (4.3%), the patients had no improvement after treatment.

The average period of hypoglycemic progress was 25.4 days, and no adverse side effects were observed, and diarrhea and the like were observed in some patients during the trial period, but after adjusting the timing of administration, symptoms improved and continued the administration.

Through the above experiments, the insulin-dependent diabetic patient administered the blood glucose lowering herbal composition according to the present invention as a test drug showed an excellent hypoglycemic effect compared to oral hypoglycemic agents or known herbal extracts and was safe in terms of toxicity and side effects. Confirmed. In addition, in the case of people using the blood sugar-lowering food composition according to the present invention, many of the symptoms associated with diabetes were found to improve shoulder pain, tingling, taste, and the like.

Experimental Example 5 Clinical Trial of Type 2 (Insulin-Independent) Diabetic Patients

The 67-year-old male living in Gumi, Gyeongsangbuk-do is a type 2 (insulin-independent) patient who has been admitted to the hospital for 8 years since 1999. In September 2007, the patient's consent was obtained, and he started taking the hypoglycemic herbal composition three times a day and five 0.4g capsules with water 30 minutes before meals.

In September 2007, when taking the herbal composition, the diabetic medicine for hospital preparation was taken twice a day, morning and evening. After 2 weeks, the blood sugar level began to decrease to 180 mg / dl, and after 3 weeks, the blood sugar level was 160 mg. decreased to / dl. From week 4, blood sugar decreased continuously after taking the herbal composition without taking hospital preparations so far in the evening, and at week 7, blood glucose was fasting 105-115mg / dl, and post-prandial blood sugar was 130-135mg. It is kept at / dl and maintains normal life.

Experimental Example 6 Clinical Trial 2 of Patients with Type 2 (Insulin-Independent) Diabetes

A 45-year-old male worker in Busan who has been aware of diabetes since 2004 and has continued to live without taking medications from hospitals. Fasting blood sugar has remained at 150mg / dl in January 2008. With the consent of the patient, the herbal composition was taken three times a day with five 0.4 g capsules at a time with water 30 minutes before meals. Fasting blood glucose levels have decreased since two weeks after taking the herbal composition, and blood glucose levels have been continuously maintained at 93 to 110 mg / dl. In particular, the appearance caused by diabetes that has been progressing has been gradually improved at the second week after taking the herbal composition, and now it is a condition that does not feel the pain caused by the appearance.

Experimental Example 7 Clinical Experiment of Type 1 (Insulin-Dependent) Diabetic Patient

A 46-year-old office worker living in Daejeon who has been treated with diabetes for about 20 years and controls insulin levels by administering 40 units of insulin a day, but blood sugar levels are about 170 mg / dl when fasting and 220 mg / dl after meals. Maintained to a degree, and always had the symptoms of fatigue and shoulder pain.

As a dosing and result, in January 2008, with the consent of the patient, the herbal composition according to the present invention was taken three times a day with five 0.4 g capsules at a time with water 30 minutes before meals.

After 8 days, fasting blood glucose improved to 135mg / dl and postprandial blood sugar was reduced to 160mg / dl, reducing insulin dose by 15 units.After 1 week, blood glucose was 130 ~ 160mg / dl and fasting blood sugar was 160 It was confirmed to be ~ 185mg / dl reduced the insulin dose by 5 units and the insulin supply was stopped only 7 weeks after taking the same method.

Since then, fasting blood sugar levels have been continuously maintained at 130-175mg / dl, and the symptoms of shoulder pain and tiredness disappeared along with the loss of the swallowing phenomenon.

From the above experimental results, Yeoju powder extract, seaweed powder extract, thaw skin powder extract, ginseng powder extract, prickly pear skin extract, lettuce extract, powder extract, goji berry powder extract, dansam powder extract, huang powder extract and banaba powder extract according to the present invention The herbal composition for lowering blood sugar was found to be a very useful herbal composition for lowering blood sugar in insulin-dependent (type 1 diabetes) and insulin-independent (type 2 diabetes) patients through animal and clinical experiments.

1-OGTT experiment of the ICR mouse of Experimental Example 1

2-Glucose change in KK-Ay mice of Experimental Example 2

3-Weight change of KK-Ay mouse of Experimental Example 2

Figure 4-Day 8 average blood glucose level of Experiment 3

5-STZ experiment of glycemic effect of Experiment 3

Claims (5)

850 to 1,024 grams of seaweed, 56 to 104 grams by weight of thaw, 56 to 104 grams by weight of ginseng, 35 to 65 grams by weight of ginseng, 84 to 156 grams by weight of thorn stem, 42 to 78 grams of lettuce skin , Blood sugar lowering herbal composition consisting of 49 to 91 grams by weight of wolfberry, 21 to 39 grams by weight of salvia, 28 to 52 grams by weight of astragalus, 105 to 195 grams of Banaba The method of claim 1, The extracts of the herbal ingredients were fermented at 70-95 ° C. in lower alcohols having 1 to 4 carbon atoms, water or mixed solvents of water and alcohols for Yeoju, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Gojija, Sweet Ginseng, Astragalus and Banaba After heating for 8 hours and then filtering and heating under reduced pressure at 60-80 ° C. to lower blood sugar, characterized in that it is a mixed extract of Yeodong, Haedongpi, Ginseng, Prickly Pear, Sangbaekpi, Gugija, Sweet Ginseng, Astragalus, Banaba Herbal composition The method of claim 1, The extract of the seaweed is added to the solvent corresponding to 8 to 10 times the weight of the seawort blended with water and ethanol in a ratio of 3: 7 and filtered by heating and heating for 5 to 8 hours at 70 to 95 ℃ 60 to 80 ℃ The mixture was dried under reduced pressure to obtain a glass powder, and the obtained powder was heated at a temperature of 450 to 500 ° C. for about 45 to 50 hours, dissolved in water corresponding to a weight ratio of 2 to 3 times the weight of the powder obtained, filtered, and then obtained 2.5 of the obtained liquid. After mixing with ethanol corresponding to the drainage and filtered by heating for 2 to 4 hours at 70 ℃ filtered and then dried by heating under reduced pressure at 60 to 80 ℃. The method of claim 1, Extract of the herbal ingredient is a herbal composition for lowering blood sugar, characterized in that the powder having a water content of 8% or less. The method of claim 1, Zinc gluconate, magnesium gluconate and potassium gluconate may be added to the extract of the herbal ingredient within the recommended daily amount of the Korea Food and Drug Administration.

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