KR101383146B1 - Composition comprising an extract of combined crude drug for preventing and treating hangover or liver disease - Google Patents

Abstract

The present invention is a complex herbal extract consisting of galhwa (칡), lettuce (odie), Schisandra chinensis, upper leaf (mulberry), geoja (hazelnut), chrysanthemum (chrysanthemum), dermis (mandarin peel), Mcmundong and sugar ginseng (mansam) It relates to a composition for the prevention and treatment of liver disease and hangover containing as an active ingredient, the extract of the present invention in inhibits ADH and ALDH enzyme levels in vitro and in In vivo , by identifying the therapeutic effects of hangover and liver disease that significantly inhibits blood levels of ethanol and Acetaldehyde, it can be used in pharmaceutical compositions for hangover prevention and prevention and treatment of liver disease.

Description

Composition comprising an extract of combined crude drug for preventing and treating hangover or liver disease}

The present invention is a complex herbal extract consisting of galhwa (칡), lettuce (odie), Schisandra chinensis, upper leaf (mulberry), geoja (hazelnut), chrysanthemum (chrysanthemum), dermis (mandarin peel), Mcmundong and sugar ginseng (mansam) It relates to a composition for relieving hangover and preventing and treating liver disease, which contains as an active ingredient.

Reference 1 K. Ebihara et al., Agri. Biol. Chem., 52, 1311, 1988.

J. Caballeria et al., Life Sci., 41, 1021-1727, 1986.

[Jeong-Han Kim, et al., Korean Journal of Agrochemicals, 38 (6), 549-553, 1995.

[Ref. 4] Jung-Seop Shin and Min-Kyo Shin, Doha Hyang-Jeom (Medicinal Herb ) Ambassador , Younglimsa, pp 704-706, 1998.

[Ref. 5] Jeong-Sup Jung and Shin Min-kyo Author, Dohae Hyang-je ( Drug Medicine), Korean Dictionary , Younglimsa, pp. 545-548, 1998.

[Ref. 6] Jung-Seop Shin and Min-Kyo Shin, Do Hae Hyang Drug (Medicinal Herb ) Ambassador , Younglimsa, pp 471-473, 1998.

[Ref. 7] Jung-Seop Shin, Min-Kyo Shin, Dohae Hyangjeomsa Dictionary , Younglim History, pp291-293, 1998.

[Ref. 8] Jung-Seop Shin and Min-Kyo Shin, Do Hae Hyang Drug (Medicinal Medicine) Metabolism , Younglimsa, pp 1039-1040, 1998.

[Ref. 9] Jung-seop Jung and Shin Min-kyo Author, Dohae Hyang-je ( Drug ) Ambassador , Younglimsa, pp. 783-785, 1998.

[Ref. 10] Information Disclosure, Chinese Medicine History Dictionary , Younglim History, pp.177-178, 1998.

[Document 11] Jung-Seop Shin and Min-Kyo Shin, Do Hae Hyang Drug (Medicinal Medicine) Ambassador , Younglimsa, pp. 1088-1089, 1998.

12. Harborne J.B. Phytochemical methods: A guide to modern techniques of plant analysis, 3rd Ed. pp 6-7, 1998.

[Reference 13] Chairman, Korea Institute of Health Procedures, Functional Test Guide for Health Functional Foods , Korea Institute of Health Procedures, pp 303-344, 2004.

In Korea, liver disease mortality rate is very high, 23.5 per 100,000 population (37.8 males and 9.0 females), and the number of deaths in South Korea is the highest (41.1 / 100,000) and the second highest among the 50 deaths (72.4 / 100,000) , The third leading cause of death in the 30s (10 people / 100,000 people), is the leading cause of death in the Korean middle-aged population. Especially alcoholic liver disease is a disease that can occur in most of chronic overdoses.

The liver is a very important organ that is responsible for various metabolism, detoxification, decomposition, synthesis and secretion in our body. First, the liver has the function of managing the energy metabolism, and metabolizes all the nutrients absorbed from food into a substance capable of producing energy, and supplies or stores the whole body. Second, the liver has a function of synthesizing, storing and distributing fat of about 2,000 kinds of enzymes, albumin, serum proteins of bile coagulation factors, bile acid, phospholipid and cholesterol. Third, the liver has a function to excrete various metabolites through the bile duct into the duodenum, and it plays an important role in maintaining our life because of its immune function. Finally, the liver has detoxification and decomposition functions to detoxify drugs, toxic substances and alcohol. However, the hepatocyte detoxification function of this liver is liable to damage the drug, toxic and alcoholic liver disease can cause.

Alcoholic liver disease can be divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis according to the clinical symptoms and usually occur when drinking 60-80g of alcohol a day for 10 years. Alcoholic fatty liver is caused by accumulation of cholesterol and triglyceride in hepatocyte due to excessive alcohol consumption. It can be recovered soon after this week, but if it continues drinking, it develops into hepatitis. Alcoholic hepatitis has necrosis and inflammation of hepatocytes, and it has various symptoms such as fatigue, anorexia, weight loss, jaundice, fever, right upper abdominal pain, and about 40% of the patients develop alcoholic cirrhosis. Alcoholic cirrhosis is a condition that is impossible to recover from normal liver, and has various symptoms such as general fatigue, decreased appetite, ascites, esophageal varices, bleeding, hepatic encephalopathy, lethargy, and poor prognosis than cirrhosis caused by hepatitis virus. In Esau, 50% of deaths from end-stage liver disease are known to be caused by alcohol.

The normal course of ethyl alcohol metabolism is the absorption of ethyl alcohol into the body is absorbed in the stomach or small intestine into the blood and then transferred to the liver. Hepatocytes have alcohol dehydrogenase (ADH), which oxidizes alcohol to acetaldehyde. The acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase in hepatocytes and transferred to muscle or fat tissue of the whole body And finally decompose into carbon dioxide and water. In addition, the acetaldehyde dehydrogenase enzyme has type Ⅱ which initiates oxidation even when acetaldehyde is low in concentration, and type Ⅰ which does not work if acetaldehyde is not concentrated in high concentration. However, Asian people generally have deficiency or lack of Ⅱ type acetaldehyde enzyme Therefore, the oxidation of acetaldehyde is slow, and therefore the normal metabolism is inhibited by the toxic action of acetaldehyde and / or ethyl alcohol which is not oxidized, and various hangover phenomenon is felt. In order to alleviate the hangover after drinking alcohol, a large number of drinks prepared by mixing or combining herbal preparations or artificial preparations have been developed.

On the other hand, ethanol is the main ingredient of alcohol and physical and mental effects on the human body has a wide range and a variety of studies on its metabolic process and toxic expression has been extensively studied for a long time. As described above, the ethanol consumed is absorbed through the small intestine and digestive tract and reaches a maximum blood concentration within 20 to 120 minutes after ingestion. The ethanol thus absorbed is metabolized in all organs including the liver, and about 10 % Are excreted through respiration or by urine and sweat, and most of the other is cleaved in the liver.

The ethanol degradation in the liver is the main metabolism through the oxidation reaction to acetaldehyde. This is known to be carried out by three reaction enzyme systems such as alcohol dehydrogenase, microsomal ethanol-oxidizing system (MEOS), and catalase (K. Ebihara et al., Agri. Biol. Chem., 52, 1311, 1988). In addition to the decomposition mechanism of ethanol, various toxicological studies have been conducted. It is reported that ethanol toxicity is not only observed from a neurological point of view, but also genetically (J. Caballeria et al., Life Sci). , 41, 1021-1727, 1986). Therefore, in recent years, research and experiments on a number of substances that can reduce the toxicity or inhibit the expression of ethanol is in progress, and as a result, various dietary supplements containing ingredients extracted from natural or herbal ingredients It is being developed in relation to the situation (Kim Jung-han et al., Korea Journal of Agricultural Chemistry, 38 (6), 549-553, 1995).

The above-mentioned alcoholic liver diseases include fatty liver, acute hepatitis, chronic hepatitis, liver cirrhosis and liver cancer. The fatty liver shows the lightest symptom and the highest incidence.

Therefore, considering the unique drinking culture of Korea, alcoholic liver disease should be properly treated in order to reduce the mortality rate of liver disease, but alcoholic liver disease treatment agent has not been developed in Korea yet.

칡 ( Pueraria thunbergian B.) is a root of perennial herb belonging to legumes (Leguminosae), and its components include isoflavone, puerarin, puerarin xylose, and diedzein. It is known to contain the sitosterol, starch, etc., and the flower of the 칡 is called a gallium (Jung Ji-seop and Shin Mingyo ) , Dohae Hyangje (Medicinal Medicine) , Yeonglimsa, pp 704-706, 1998).

Morus alba L.) is a deciduous tree belonging to the Moraceae family, and the mulberry leaves are called the upper leaves, and the root bark from which the cork layer of the root bark has been removed is the upper bark (ordinary bark), the branches are upper, the fruits are lower (audi), and the seeds are lower It is called each of the leaves, and the essential ingredient of rutin, quercetin, moracetin, eugenol and the like, fatty acids such as palmitic acid, trigonelline, etc. Ingredients are known (Information and Shin Min-kyo ) , Dohae Hyangje (Medicinal Herbs ) , Yeonglimsa, pp 545-548, 1998).

Schisandra chinenesis B.) is a deciduous tree belonging to the Magnoliaceae. The fruit of Schisandra chinensis and its related plants is called Schisandra chinensis and deoxyschizandrin, schizandrin, sesquikarene (Schisandrarin) sesquicarene) it is known components of the essential oil component, such as bar, such as a (jeongboseop and sinmingyo low, illustrated hyangyak (herbal) Dictionary, Younglim four, pp 471-473, 1998).

Hovenia dulcis T. is a deciduous tree belonging to the family of Rhamnaceae. The bark of the Hovenia dulcis and the same plant is the bark of the bark, the earth's mouth, the roots of the earth, the fruit of the earth. It is called each and grows, and it contains flagugulanine, hovenine, hobenoside, etc., and it is known to treat indigestion and arthritis. Hyangjedae Dictionary, Younglimsa, pp291-293, 1998).

Chrysanthemum morifolium R.) is a perennial herb belonging to Compositae, and the flower part of the chrysanthemum is called chrysanthemum, and its components are chrysanthemin, amino acid, adenine, and luteolin-7-glucose. Components such as essential oils such as luteolin-7-glucoside, chrysanthenone, cosmosiin, and acaciin have been known (Information and Shinmingyo ) , Younglim, pp 1039-1040, 1998).

Citrus unshiu M.) is an evergreen sub-tree belonging to the Rutaceae family, with the fruits of tangerine and the same plant being added, the skins of immature fruits called green skins, the mature skins called tangerines or dermis, the seeds tangerine cores, and the tangerine leaves. with hesperidin (hesperidin), Military acid, ball acid, sachet Barros (sacebarose), crypto-xanthine (cryptoxanthin), are known components of the vitamin B, C (jeongboseop and sinmingyo low, illustrated hyangyak (herbal) Dictionary, Younglim four , pp 783-785, 1998).

Maekmundong (Liriope rhizoma) is a perennial plant belonging to the family Liliaceae maekmundong (Liriope platyphylla) or more maekmundong (Ophiopogon Japonicus ) is a dried tuber that contains ingredients such as sitosterol, stigmaterol, ophioside, and is known to have pharmacological effects such as antimicrobial activity and hypoglycemic activity. (Information Interference Pharmacological Dictionary, Younglimsa, p177-178, 1998 year).

Ten thousand and three (Conodopsis pilosula N.) has been used for the treatment of a perennial plant belonging to campanulaceae (Campanulaceae), referred to as the root of ten thousand and three dangsam (ten thousand and three), loss of appetite, dry mouth, diarrhea (jeongboseop and sinmingyo me, illustrations hyangyak ( Drug) Daesaeng, Yeonglimsa, pp 1088-1089, 1998).

However, even in the above literature, a compound herb consisting of brown flowers, mulberry, mulberry, lettuce leaves, mulberry leaves, chrysanthemum, chrysanthemum, dermis, tangerine, and ginseng There is no disclosure or teaching on the effect on liver disease and hangover relief containing the extract as an active ingredient.

The inventors of the present invention is a combination preparation extract in inhibits ADH and ALDH enzyme levels in vitro and in In vivo , the present invention was completed by confirming a therapeutic effect on hangover and liver disease that significantly inhibit blood levels of Ethanol and Acetaldehyde.

In order to achieve the above object, the present invention is a pharmaceutical for the prevention and treatment of hangover and liver disease containing a complex herbal extract consisting of brown hair, lettuce, Schisandra chinensis, upper leaf, earth, chrysanthemum, dermis, Macmundong and Ginseng as an active ingredient To provide a composition.

Specifically, the compound herbal as defined herein has a relative weight compound (w / w) ratio of brown, chopped, schizandra, lettuce, earth, chrysanthemum, dermis, macmundong and ginseng from 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10, preferably, 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5, more preferably 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2 : It is characterized by blending in the compounding ratio of 1-2.

Extracts as defined herein are extract compounds available in a solvent selected from water, methanol, ethanol, butanol or a mixed solvent thereof, preferably water, methanol, butanol or a mixed solvent thereof, more preferably water and ethanol mixed Water extracts soluble in a solvent, even more preferably water.

Liver diseases as defined herein include autoimmune liver disease, drug-induced liver disease, alcoholic liver disease, nonalcoholic liver disease, infectious liver disease, congenital metabolic liver disease, acute hepatitis, chronic hepatitis, liver cirrhosis, cirrhosis, fatty liver or liver cancer, Preferably, alcoholic liver disease or non-alcoholic liver disease is included.

Hereinafter, the method for obtaining the extract of the present invention will be described in detail.

For example, the extract is a raw material of the dried state of brown, heart, Schizandra chinensis, lettuce, earth, chrysanthemum, dermis, Macmundong and Ginseng in a certain ratio, preferably, the relative ratio (w / w) of 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10, preferably, 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5, more preferably 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2 : 1 to 2: 1 to 2: blending in a blending ratio of 1 to 2: 1; Water, a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, preferably water and an ethanol mixed solvent, is added by about 1 to 5 times, preferably 2 to 4 times the weight of the dried raw material, More preferably at room temperature or at 80 占 폚 for 10 to 30 hours, preferably for about 18 to 25 hours, at a temperature of 5 to 100 占 폚, preferably 20 to 90 占 폚, Or a heat extraction method, preferably a cold extraction method, followed by filtration and concentration under reduced pressure to obtain the extract of the present invention.

It is also possible to further carry out conventional fractionation processes (Harborne JB Phytochemical methods: A guide to modern techniques of plant analysis, 3rd Ed. Pp 6-7, 1998).

Accordingly, the present invention is a hangover relief and prevention of liver disease containing a complex herbal extract consisting of browning, lettuce, Schisandra chinensis, upper leaf, earth, chrysanthemum, dermis, Macmundong and Ginseng as an active ingredient obtained by the production method and the manufacturing method And it provides a pharmaceutical composition for treatment.

The combination extract prepared above is in inhibits ADH and ALDH enzyme levels in vitro and in In vivo, the effects of ethanol and Acetaldehyde on hangover and liver disease were significantly inhibited.

The pharmaceutical composition of the present invention comprises 0.1 to 50% by weight of the above extract, based on the total weight of the composition.

However, the composition is not limited thereto, and may vary depending on the condition of the patient, the type of disease, and the progress of the disease.

Since the extract of the present invention has little toxicity and side effects, it can be safely used even for long-term administration for the purpose of prevention.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The composition of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions, Examples of carriers, excipients and diluents that can be included in the composition containing the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of suppository bases include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at 0.5 g / kg to 5 g / kg, preferably 1 g / kg to 3 g / kg per day. The administration may be carried out once a day or divided into several doses. Thus, the dosage amounts are not intended to limit the scope of the invention in any manner.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

In another aspect, the present invention provides a health functional food for the hangover and the prevention and improvement of liver disease, containing a complex herbal extract consisting of brown hair, lettuce, Schisandra chinensis, upper leaf, earth, chrysanthemum, dermis, Macmundong and Ginseng as an active ingredient. .

&Quot; Health functional food "as defined herein means food prepared and processed using raw materials or ingredients having functionality useful to the human body in accordance with Law No. 6727 on Health Functional Foods." Functional " Structure and function of the nutrient to control or physiological effects, such as to obtain a beneficial effect for health is intended to eat.

Health functional food for the hangover and liver disease prevention of the present invention, the extract comprises 0.01 to 95% by weight, preferably 1 to 80% by weight relative to the total weight of the composition.

In addition, it is possible to manufacture and process as a health functional food in the form of tablets, capsules, powders, granules, liquids, pills for the purpose of relieving hangovers and preventing liver diseases.

The present invention provides a dietary supplement comprising a complex herbal extract consisting of the browning, lettuce, Schisandra chinensis, lettuce, earth, chrysanthemum, dermis, myeongmundong and ginseng showing the effect of relieving hangover and preventing and improving liver disease. Examples of foods to which the extract can be added include various foods, beverages, gums, tea, vitamin complexes, and health functional foods.

The composition comprising the extract of the present invention can be used in various ways, such as drugs, foods and beverages for hangover relief and prevention and improvement of liver disease. Examples of the foods to which the extract of the present invention can be added include various foods, beverages, gums, tea, vitamin complexes, health supplements and the like, and they can be used as powders, granules, tablets, capsules or beverages have.

In addition, the present invention is a food or food additive containing a complex herbal extract consisting of browning, lettuce, Schisandra chinensis, lettuce, earth, chrysanthemum, dermis, Macmundong, and Ginseng as a main component to relieve hangover and prevent and improve liver disease to provide. Extract of the present invention can be added to food or beverages for the purpose of relieving hangovers and preventing and improving liver disease. At this time, the amount of the extract in the food or beverage is generally the health food composition of the present invention can be added to 1 to 5% by weight of the total food weight, the health beverage composition is 0.02 to 10 g, preferably based on 100 ml Can be added in a ratio of 0.3 to 1 g.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient, as long as it contains the extract as an essential ingredient at the indicated ratio, and there is no particular limitation to the liquid ingredient. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like Sugar, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. have. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 mL of the composition of the present invention.

It is preferable to contain the composition for hangover decay of the present invention.

In addition, the present invention provides a hangover chew gum comprising the composition for hangover resolution according to the present invention. For example, the hangover gum may be prepared by adding to the composition for minor hangover according to the present invention the general components necessary for the production of gum, such as gum base and saccharides such as sugar, glucose, isomalt or xylitol, Can be prepared according to the usual known methods. In order to smooth the physical properties of the gum and to make the gum cohesive, saccharide syrups such as, for example, Soltitol 80, starch syrup, maltitol syrup or sorbitol syrup may be added. In addition, at least one selected from the group consisting of fragrances, stabilizers, pigments, emulsifiers and brighteners can be further added. The fragrance is added to excellence of the gum flavor, and a fragrance such as a mint flavor, a fruit flavor or a herb flavor can be used. The stabilizer may be gum arabic, gelatin, carageenan, or the like. With the addition of such stabilizers, the freshness can be maintained, the shape can be preserved, and the texture and texture of the gum can be enhanced against changes during heating or preservation during processing. In addition, the hangover chewing gum according to the present invention may be prepared by further adding natural coloring matters or synthetic coloring matters such as a flower gum, a gardenia yellow color, a caramel color or a grape skin extract pigment to enhance visual effects. Furthermore, emulsifying agents such as sucrose fatty acid esters or lecithin and polishes such as carnauba wax or shellac are further added to enhance the visual effect of the gum to uniformly disperse the flavor in the sugar solution, . The chewable gum according to the present invention preferably contains 50-500 mg, more preferably 150 mg, of the composition for a hangover remedy according to the present invention per 1.6 g of gum.

The composition for hangover decay according to the present invention may be formulated into various forms such as suspensions, pills, tablets, capsules, powders and the like, which are convenient to take in addition to the beverage and gum. Such formulations may be prepared using conventional methods known in the art

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and vegetable beverages. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Extract of the present invention in In vitro, ADH and ALDH enzyme levels are inhibited, and in vivo, ethanol and Acetaldehyde significantly inhibit blood levels of hangover and liver disease. It can be usefully used.

1 is a view showing the effect on the alcohol resolution of the combination preparation extract,
Figure 2 is a diagram showing the inhibitory activity against acetaldehyde level of the combination preparation extract,
Figure 3 is a diagram showing the activity of ADH (alcohol dehydrogenase) and ALDH in the blood of the combination preparation extract.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

Example  1. Preparation of complex preparation extract

Gallium (18,000 g), Sangsim (18,000 g), Schisandra chinensis (18,000 g), Lettuce (18,000 g), Seongja (18,000 g), Chrysanthemum (18,000 g), Dermis (18,000 g), Macmundong (18,000 g) and 1,500 L of water were added to Ginseng (18,000 g), followed by extraction at 100 ° C. for 6 hours to obtain an extract (hereinafter referred to as KRW).

Reference Example 1. Preparation for experiment

1-1. Sample Preparation

The combination formulation extract was used as a sample for use in the experiment.

1-2. Experimental animal

The experimental animals used for the experiment were magnetic Sprague Dawley rats weighing 200-250 g. The samples were stabilized for 48 hours by free intake of solid samples and tap water, and used for the test after two weeks of pre-breeding.

1-3. Experimental group

Experiments were performed by dividing the experimental groups as follows. (See Table 1)

Experimental group and treatment Group name Dose Dosing volume (ml) Sample preparation concentration Untreated group 0 2 - Alcohol treatment group 0 2 - KRW 10ml / kg 2 2ml dose of product A famous A company product (hereinafter referred to as YM) 10ml / kg 2 2ml dose of product

1-4. Statistical processing

All experimental results were statistically analyzed using one way analysis of variance (ANOVA), and significance was tested at p <0.05 level using the Student-Newman-Keuls Test if significant. Was carried out.

Experimental Example 1. Determination of alcohol resolution

In order to determine the alcohol degradation activity in the rat of the sample obtained in the above example, the experiment was performed using the method described in the existing literature as follows (Research Chairman, Korea Institute of Health and Procedure , Functional Test Guide of Health Functional Food) , Korea Institute of Health Process, pp 303-344, 2004).

1-1. Blood Alcohol  Concentration measurement

In order to measure the hangover relief effect of the extract obtained in Example 1, the extract was administered to the experimental animal ingested alcohol to measure the blood alcohol concentration. Specifically, the experimental animals used a magnetic Sprague Dawley rat weighing 200-250 g, and used for the test after preliminary breeding for two weeks by allowing free intake of solid samples and tap water. After 18 hours of fasting, the test group was orally administered with a test sample at a concentration of 10 ml / Kg, and the control group was administered only 10 ml / kg of water. Oral administration was forced oral administration using stainless steel sonde (length, 10 cm). Thirty minutes after the test sample administration, 2 ml of 40% ethanol was forced orally administered using stainless steel sonde (length, 10 cm). 1 hour, 3 hours, 5 hours, 3 times, 1 hour, 3 hours after cutting the tail, blood was cut by cutting the tail, 5 hours were collected using a syringe in the abdominal aorta and the blood collected was 20 minutes at 3000rpm Serum was separated by centrifugation. The separated serum was measured at wavelength 340nm using Ethanol Kit (Sigma, USA).

1-2. Blood Acetaldehyde Level Measurement

Acetaldehyde dehydrogenase activity was measured by the rate of NADH production at an absorbance of 340 nm. The composition of the reaction solution was cuvetted with 2 ml of distilled water, 0.3 ml of 1 M tris-HCl (pH 8.0), 20 mM NAD + 0.1 ml, 0.1 ml of 1 M acetaldehyde, 0.1 ml of sample and 0.1 ml of enzyme source. The reaction was adjusted to a total of 3 ml and reacted for 5 minutes at 30 ° C., and then the change in absorbance at 340 nm was measured for 5 minutes. At this time, the acetaldehyde dehydrogenase activity of the sample was measured as the relative activity (%) relative to the control group was added as a control.

As a result of the experiment, as shown in Figures 1 and 2, the composite formulation of the present invention is superior to the hangover remedy products on the market and exhibits excellent alcohol resolution and acetaldehyde-reducing activity, the composition of the present invention to hangover It could be useful.

Experimental Example  2. Serum ADH  ( Alcohol dehydrogenase ) And ALDH  Active measurement ( in vitro )

In order to determine the inhibitory effect on the blood ADH (alcohol dehydrogenase) and ALDH (acetaldehyde dehydrogenase) of the sample obtained in the above example, the experiment was carried out as follows using the method described in the existing literature (Re) Chairman of the Society, Functional Test Guide for Health Functional Foods , Korea Institute of Health Procedures, pp 303-344, 2004).

2-1. Blood ADH ( Alcohol dehydrogenase ) Active measurement

The enzyme source was 26.4 mg of rat liver homogenate (MOLTOX Co., USA) of lyophilized S9 rats (final concentration was 3.3 mg / ml) and 8 ml of 0.1% BSA (bovine serum albumin) solution. Dissolved in and filtered through a 0.45 μm syringe filter. Alcohol dehydrogenase activity was measured using the rate of NADH production as absorbance at 340 nm. The reaction mixture consisted of 1 ml of distilled water, 0.75 ml of 1 M tris-HCl (pH 8.8), 20 nM NAD + 0.3 ml, 0.3 ml of ethanol, 0.1 ml of sample and 0.15 ml of enzyme source in a cuvette. After adjusting to 3 ml and prereacting at 30 ° C. for 5 minutes, the change in absorbance was measured at 340 nm for 5 minutes. At this time, the sample was not added as a control. Alcohol dehydrogenase activity of the sample was measured as relative activity (%) relative to the control.

2-2. ALDH  ( acetaldehyde dehydrogenase Inhibitory activity against

The enzyme source was 26.4 mg of rat liver homogenate (MOLTOX Co., USA) of lyophilized S9 rats (final concentration was 3.3 mg / ml) and 8 ml of 0.1% BSA (bovine serum albumin) solution. Dissolved in and filtered through a 0.45 μm syringe filter. The absorption rate of NADH at 340 nm was used as an index. The composition of the reaction solution was 2.1 ml of distilled water, 0.3 ml of 1.0 M tris-HCl buffer (PH 8.0), 20 mM NAD ⁺ 0.1 ml, 0.1 ml of 1.0 M acetaldehyde, 0.1 ml of 3.0 M KCl, 0.1 ml of 0.33 M 2-mercaptoethanol, and a sample. 0.1 ml and 0.1 ml of enzyme source were put in a cuvette, adjusted to a total of 3 ml, pre-reacted at 30 ° C. for 5 minutes, and then the change in absorbance at 340 nm was measured for 5 minutes. At this time, the sample was not added as a control. The ALDH activity of the sample was measured as the control relative activity (%).

As can be seen in Figure 3, the combination of the present invention exhibits superior inhibitory activity against blood ADH (alcohol dehydrogenase) and ALDH (acetaldehyde dehydrogenase) in the blood than the famous hangover treatment products on the market, the composition of the present invention promotes alcohol metabolism It can be seen.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but will be specifically described.

Preparation Example 1. Preparation of powder

KRW Extract 20 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation Example 2. Preparation of tablets

KRW Extract 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

KRW Extract 10 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

KRW Extract 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

(2 ml) per 1 ampoule according to the usual injection preparation method.

Formulation example  5. Liquid  Produce

KRW Extract 20 mg

10 g per isomer

5 g mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Claims (9)

A pharmaceutical composition for hangover elimination containing as an active ingredient a complex herbal extract consisting of brown flower, lettuce, Schisandra chinensis, lettuce, earth, chrysanthemum, dermis, macmundong and ginseng. The method of claim 1, wherein the compound herbal compound has a relative weight compound (w / w) ratio of brown, chopped, Schizandra chinensis, lettuce, earth, chrysanthemum, dermis, ginmundong and sugar ginseng 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10 pharmaceutical composition. The pharmaceutical composition according to claim 1, wherein the extract is soluble in a solvent selected from water, methanol, ethanol, butanol or a mixed solvent thereof. delete Health functional food for hangover elimination containing as an active ingredient a complex herbal extract consisting of brown flower, lettuce, Schisandra chinensis, lettuce, earth, chrysanthemum, dermis, macmundong and ginseng. The health functional food according to claim 5, wherein the health functional food is in tablet, capsule, powder, granule, liquid, or pill form. Health supplements containing complex herbal extracts composed of brown, lettuce, Schisandra chinensis, lettuce, earth, chrysanthemum, dermis, macmundong and ginseng showing hangover effect. 8. The dietary supplement of claim 7, wherein the dietary supplement is in the form of a powder, granule, tablet, capsule, beverage, gum, tea, vitamin complex. delete

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