CN114642709B - Traditional Chinese medicine composition and extract for improving reproductive performance of later laying period of breeding hens and preparation method thereof - Google Patents
Traditional Chinese medicine composition and extract for improving reproductive performance of later laying period of breeding hens and preparation method thereof Download PDFInfo
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Abstract
The application discloses a formula for improving reproductive performance of a breeding hen at the later laying period, an extract and a preparation method thereof, wherein the formula comprises the following components in percentage by mass: 3-2 parts of radix fici simplicissimae, 3-2 parts of polygonatum odoratum, 2.5-1.5 parts of red paeony root, 2.5-1.5 parts of Chenopodium hybridum and 1.5-0.5 part of bergenia purpurascens. The Chinese medicinal formula has the technical effects that the five-finger wild peach and the fragrant solomonseal rhizome are used as monarch medicaments, the red paeony root is used as ministerial medicaments, the Chinese worries are used as adjuvant medicaments, and the bergenia purpurascens is used as a messenger medicament, so that the Chinese medicinal formula can play the effects of tonifying spleen and lung, nourishing yin and moistening dryness, and simultaneously activating blood circulation to dissipate blood stasis and strengthening the body, and has better effects on yin deficiency, blood stasis and inflammation of genital tract in the later stage of the laying hens.
Description
Technical Field
The application belongs to the technical field of feed additives, and particularly relates to a formula for improving reproductive performance of breeding hens in the later period of egg laying, an extract and a preparation method thereof.
Background
In recent years, with the rapid improvement of the modern breeding industry level in China and the improvement of the living standard of people, the demand of the public on eggs, meat and milk is increased year by year, and most importantly, the requirements of people on the quality of the eggs, the meat and the milk are higher and higher, so that the production of green healthy food, the reasonable development and utilization of resources and the protection of natural ecological environment become the development trend of a new era. The feed safety and food safety become core problems of concern at home and abroad, chemically synthesized drug additives such as antibiotics and the like play a great role in promoting animal production, however, with the abuse and excessive use of antibiotics, the drug residues in livestock and poultry products seriously exceed the standard, and meanwhile, the damage to human bodies is gradually revealed, so the desire of people for seeking new green harmless feed additives is more and more urgent. People make various efforts from different angles to improve the production performance of breeding hens, and Chinese medicaments have attracted the attention of researchers at home and abroad because the Chinese medicaments have the effects of no residue, no drug resistance and green safety when used in animal production. Therefore, a new technical scheme of the traditional Chinese medicine composition for improving the reproductive performance of the later-stage laying hens is needed.
Disclosure of Invention
One purpose of the application is to provide a new technical scheme of the traditional Chinese medicine composition for improving the reproductive performance of the breeding hens in the later period of laying.
According to one aspect of the application, the application provides a traditional Chinese medicine composition for improving the reproductive performance of the later laying period of breeding hens, which comprises the following components in parts by mass: 2-3 parts of radix fici simplicissimae, 2-3 parts of polygonatum odoratum, 1.5-2.5 parts of red paeony root, 1.5-2.5 parts of Chenopodium hybridum and 0.5-1.5 parts of bergenia purpurascens.
Optionally, the traditional Chinese medicine composition comprises the following components in parts by mass: the method comprises the following steps: 2.5 parts of radix fici simplicissimae, 2.5 parts of radix polygonati officinalis, 2 parts of radix paeoniae rubra, 2 parts of folium chenopodii and 1 part of bergenia purpurascens.
According to another aspect of the application, the application also provides a traditional Chinese medicine extract for improving the reproductive performance of the later laying hens, wherein the traditional Chinese medicine extract is extracted from the traditional Chinese medicine composition in the traditional Chinese medicine formula.
According to another aspect of the present application, the present application also provides a preparation method of the traditional Chinese medicine extract for improving the later-stage reproductive performance of the laying hens, which comprises the following steps:
mixing Ficus Simplicissima lour, rhizoma Polygonati Odorati, radix Paeoniae Rubra, herba Chenopodii Hybridi and rhizoma Seu herba Bergeniae at a certain mass ratio to obtain a Chinese medicinal mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution;
and carrying out spray drying on the extracting solution to obtain the traditional Chinese medicine extract.
Optionally, the technological parameters of ultrasonic extraction are that the volume mass ratio of the extraction solvent to the traditional Chinese medicine mixture is 40-1mL/g, the extraction temperature is 45-55 ℃, the ultrasonic time is 25-35min, and the ultrasonic power is 950-1050W.
Optionally, the extraction solvent is water.
Optionally, the ultrasound extraction is performed twice on the traditional Chinese medicine mixture using ultrasound.
Optionally, the spray drying process parameters are that the inlet air temperature is 120-130 ℃, and the inlet air volume is 15 DEG C-25 m 3 The sampling speed of the extracting solution is 5-10mL/min.
Optionally, mixing 3-2 parts of Ficus Simplicissima lour, 3-2 parts of rhizoma Polygonati Odorati, 2.5-1.5 parts of radix Paeoniae Rubra, 2.5-1.5 parts of Chenopodium Hybridum and 1.5-0.5 part of rhizoma Seu herba Bergeniae by mass to obtain a Chinese medicinal mixture.
Optionally, mixing 2.5 parts of Ficus Simplicissima lour, 2.5 parts of rhizoma Polygonati Odorati, 2 parts of radix Paeoniae Rubra, 2 parts of Chenopodium Hybridum and 1 part of rhizoma Seu herba Bergeniae in a mass ratio to obtain a Chinese medicinal mixture.
The Chinese medicinal formula has the technical effects that the five-finger wild peach and the fragrant solomonseal rhizome are used as monarch medicaments, the red paeony root is used as ministerial medicaments, the Chinese worries are used as adjuvant medicaments, and the bergenia purpurascens is used as a messenger medicament, so that the Chinese medicinal formula can play the effects of tonifying spleen and lung, nourishing yin and moistening dryness, and simultaneously activating blood circulation to dissipate blood stasis and strengthening the body, and has better effects on yin deficiency, blood stasis and inflammation of genital tract in the later stage of the laying hens.
Detailed Description
The following embodiments of the present application will be described in detail with reference to the accompanying examples, so that the implementation process of how to apply the technical means to solve the technical problems and achieve the technical effects of the present application can be fully understood and implemented.
The application provides a traditional Chinese medicine composition for improving reproductive performance of later-stage laying hens, which comprises the following components in parts by mass in some embodiments: 2-3 parts of radix fici simplicissimae, 2-3 parts of radix polygonati officinalis, 1.5-2.5 parts of radix paeoniae rubra, 1.5-2.5 parts of chenopodium hybridum and 0.5-1.5 parts of bergenia purpurascens.
Ficus hirta Vahl, root of Ficus hirta of Ficus of Moraceae. The plant Ficus benjamina is distributed in the south and southwest of China. Sweet in flavor and mild in nature. Meridians enter spleen, lung and liver meridians. Has the effects of invigorating spleen and lung, promoting qi circulation, removing dampness, relaxing muscles and tendons, and activating collaterals. It can be used for treating cough due to pulmonary tuberculosis, night sweat, asthenia, anorexia, abdominal distention, edema, rheumatalgia, hepatitis, leucorrhea, and puerperal agalactia. The hispid fig has certain medicinal value, the medicinal part is mainly root, and the medicinal components are mainly psoralen, bergapten and the like. In recent years, various scholars have analyzed the chemical components of hispid fig. Extracting various volatile components from Ficus Simplicissima lour by ether extraction or steam distillation, and analyzing by gas chromatography-mass spectrometry (GC-MS); or separating and purifying chemical components by various chromatography methods, and identifying the structure of the separated compound. Through the method, the chemical components in the hispid fig root are found to be flavonoid, coumarin, terpenes and the like. The radix fici simplicissimae medicinal material contains mineral elements such as Ca, mg, mn, fe, zn, cu and the like which are all essential elements of a human body, and is beneficial to disease defense and normal growth and development of the human body. Ca. Mn, fe and Zn have effects of nourishing blood, benefiting liver and invigorating kidney; mn, zn and Cu can promote growth and development and improve human immunity; in addition, mn also has antiaging and anticancer effects. Rhizoma Polygonati Odorati is dried rhizome of Polygonatum odoratum (Mill.) Druce of Liliaceae. Sweet in flavor and slightly cold in nature. It enters lung and stomach meridians. Has the main functions of nourishing yin, moistening dryness, promoting the production of body fluid and quenching thirst. Can be used for treating yin injury of lung and stomach, cough due to dryness-heat, dry throat, thirst, and diabetes due to internal heat. Modern researches find that small dose of decoction and tincture of rhizoma Polygonati Odorati on heart can rapidly enhance isolated frog heart beat, and large dose can weaken or even stop heart beat; the contractility to the isolated rabbit heart is firstly inhibited and then excited, and the heart rate is not influenced; has no obvious effect on the contractility and the heart rate of the rabbit in situ heart; has certain protection effect on rabbit acute myocardial ischemia caused by pituitrin. When 1-5 mL of 20% polygonatum decoction is injected into the vein of the rabbit, the blood pressure is slowly increased. When the polygonatum extract is orally taken, the blood sugar rises first and then falls, the polygonatum extract has obvious inhibition effect on hyperglycemia caused by epinephrine and also has inhibition effect on hyperglycemia of rats caused by glucose and alloxan. The Yuzhu decoction has a slight stimulation effect on isolated uterus of mice, and can firstly enhance the intestinal tract activity of mice, and then gradually relax and reduce the peristalsis. The rhizoma Polygonati Odorati decoction is administered by intragastric administration, and has effects of reducing triglyceride, cholesterol and beta-lipoprotein of experimental hyperlipidemia rabbit. The polygonatum odoratum injection is perfused into the stomach or injected into the muscle, has the effect of preventing the triglyceride from rising, and the cholesterol is obviously increased after the administration compared with that before the administration; has certain relieving effect on the formation of animal atherosclerotic plaques. The radix fici simplicissimae and the radix polygonati officinalis are used as monarch drugs and can play the effects of strengthening spleen, tonifying lung, nourishing yin and moistening dryness. The later stage of the laying hens, namely deficiency of qi and blood, weakness of spleen and lung and heat generation due to yin deficiency, can be just treated by the hispid fig and the polygonatum.
Radix Paeoniae Rubra, root of Paeonia veitchii Lynch of Ranunculaceae. Bitter and slightly cold. It enters liver meridian. Has the effects of clearing heat, cooling blood, promoting blood circulation and removing blood stasis. The red peony root also has the function of improving the rheological property of blood for patients with high-viscosity coronary heart disease, so that the whole blood viscosity is reduced at medium and low cutting speeds, the red blood cell electrophoresis time is prolonged, and the platelet aggregation is reduced. The inhibition of platelet aggregation by paeonia lactiflora essence is related to the aggregation promotion effect on resisting TXA 2. The intravenous drip of radix Paeoniae Rubra extract for patients with coronary heart disease and angina pectoris can significantly reduce the percentage and aggregation number of platelet with enlarged aggregation, which indicates that the surface activity and aggregation of platelet with increased activity are significantly reduced. The experiments show that the mechanism of inhibiting platelet aggregation by the red paeony root is probably related to the interference of platelet energy metabolism, the inhibition of TXA2 biosynthesis, the increase of cAMP content in platelets and the like. The mechanism of red peony root against arteriosclerosis may be related to improving the ratio of lipoprotein components, reducing plasma lipid peroxide, reducing calcium deposition on the arterial wall, inhibiting platelet aggregation, regulating TXA2/PGI2 balance and activating adenylate cyclase to increase cAMP concentration in the arterial wall, etc. The red peony root injection with the liver protection effect is 3.3mg/ml,1.67mg/ml and 0.7mg/ml, has obvious promotion effect on DNA synthesis of in vitro cultured liver cells, and has good influence on liver cell regeneration and liver function recovery. The red peony injection of 3.75g/kg is injected intravenously, has obvious protective effect on the liver injury of rats caused by D-galactosamine, increases the survival rate of animals, obviously reduces the liver atrophy and alanine aminotransferase than a control group, and increases FN (main opsonin of a mononuclear-macrophage system) than the control group. The liver protection mechanism may be to increase the level of Plasma Fibronectin (PFN) in rats, thereby enhancing the phagocytic function of the reticuloendothelial system and enhancing the opsonin activity, so as to protect liver cells, prevent the immune damage of the liver and promote the regeneration of the liver cells. Radix Paeoniae Rubra has strong inhibitory effect on dysentery bacillus, typhoid bacillus and hemolytic streptococcus in vitro. The herbaceous peony decoction is used for gastric lavage, which can temporarily increase the blood sugar of rabbits, the liquid-alcohol extract of the herbaceous peony with narrow leaves has obvious sedative and analgesic effects, and can also increase the acidity of gastric juice, promote appetite and digestion. The red paeony root is used as a ministerial drug in the formula, has the effects of cooling blood, removing stasis and relieving pain, and helps the breeding hens to eliminate blood stasis caused by qi and blood stasis in the later period of egg laying and salpingitis.
Chenopodium polycarpum (Chenopodium hybridum) is a whole plant of Chenopodium polycephalum of Chenopodiaceae. Bitter, pungent and mild. It enters liver, stomach and large intestine meridians. The main functional indications are as follows: stop bleeding, activate blood. For irregular menstruation, metrorrhagia and metrostaxis, hemoptysis, epistaxis, hematuria, sore and carbuncle pyogenic infections, and Chenopodium Hybridum main ingredient (Pogostemon bodies) have obvious inhibiting effect on white fungus, cryptococcus neoformans, rhizopus nigricans and other fungi, and also have certain inhibiting effect on alpha hemolytic streptococcus and other bacteria. The growth of staphylococcus aureus, staphylococcus albus and bacillus subtilis also has a definite inhibiting effect. The total saponins of herba Agastaches can inhibit rat foot swelling caused by carrageenan, resist mouse skin capillary permeability increase caused by histamine, and excite adrenal cortex function. Chenopodium hybridum is used as adjuvant drug in the prescription to assist radix Paeoniae Rubra in stopping bleeding and promoting blood circulation.
Bergenia purpurascens (Bergenia purpurascens, bergenia) is rhizome or whole herb of Bergenia of Saxifragaceae. It is used for eliminating phlegm, relieving cough, astringing and stopping bleeding in old times, and is used as tonic to treat deficiency and dizziness. This herb is bitter and astringent in taste and neutral in nature; it enters lung, liver and spleen meridians. Has the functions of eliminating phlegm, relieving cough, astringing to stop bleeding and stopping diarrhea. Can be used for treating cough, hematemesis, hemoptysis, hematochezia, functional metrorrhagia, traumatic hemorrhage, diarrhea, dysentery, stranguria with turbid urine, and leucorrhea with red and white discharge. The bergenia purpurascens has a certain cough relieving effect on mice, and the effect of 300mg/kg of rhizome is equal to that of 60mg/kg of codeine phosphate. Bergenin contained in rhizoma Seu herba Bergeniae also has antitussive effect. Rhizoma Seu herba Bergeniae decoction and ethanol extract (mainly containing condensed tannin and bergenin) have no antibacterial effect on Escherichia coli, typhoid bacillus and paratyphoid bacillus; has antibacterial effects on four dysentery bacillus (Shigella flexneri, shigella shinei, sonnei shigella, and Shigella baumannii) at different degrees; the shigella flexneri inhibitor has the strongest inhibition effect on shigella flexneri. Bergenia infusion in test tube 1: the concentration of 320-480 can inhibit dysentery bacillus. The ursolic glycoside contained in bergenia cordifolia has anti-inflammatory effect, and the tannin has astringent effect, so the bergenia cordifolia is commonly used for treating non-infectious inflammation of digestive tract in clinic. The bergenia crassifolia can be used as a guiding drug in the formula, not only can play a role of astringency and hemostasis, but also can guide other traditional Chinese medicines to the damaged parts of the breeding hens.
The traditional Chinese medicine formula taking the hispid fig and the polygonatum as monarch drugs, the red paeony root as ministerial drugs, the chenopodium polycarpum as adjuvant drugs and the bergenia purpurascens as messenger drugs can play the effects of tonifying spleen and lung, nourishing yin and moistening dryness, and simultaneously activating blood circulation to dissipate blood stasis and strengthening the body, and has better effects on the later-stage yin deficiency, blood stasis and inflammation of reproductive tract of the laying hens.
Optionally, the coating comprises the following components in parts by mass: 2.5 parts of radix fici simplicissimae, 2.5 parts of radix polygonati officinalis, 2 parts of radix paeoniae rubra, 2 parts of chenopodium hybridum and 1 part of bergenia purpurascens.
The traditional Chinese medicine extract for improving the reproductive performance of the later laying period of the breeding hens is extracted from the traditional Chinese medicine composition in the traditional Chinese medicine formula.
The preparation method of the traditional Chinese medicine extract for improving the later-stage reproductive performance of the laying hens, which is provided by the application, comprises the following steps:
mixing radix fici simplicissimae, rhizoma Polygonati Odorati, radix Paeoniae Rubra, herba Chenopodii Hybridi and rhizoma Seu herba Bergeniae at a certain mass ratio to obtain a Chinese medicinal mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution; the technological parameters of ultrasonic extraction are that the volume mass ratio of an extraction solvent (water) to a traditional Chinese medicine mixture is 40-1mL/g, the extract is water, the extraction temperature is 45-55 ℃, the ultrasonic time is 25-35min, and the ultrasonic power is 950-1050W. Performing ultrasonic extraction on the traditional Chinese medicine mixture twice by using ultrasonic waves.
And carrying out spray drying on the extracting solution to obtain the traditional Chinese medicine extract. The technological parameters of the spray drying are that the inlet air temperature is 120-130 ℃, and the inlet air volume is 15-25m 3 The sampling speed of the extracting solution is 5-10mL/min.
The effective components such as polysaccharide and the like are extracted by adopting ultrasonic waves, the time is short, the loss is small, and meanwhile, the spray drying is favorable for storing and avoiding the loss of medicinal components.
Example 1
A traditional Chinese medicine composition for improving reproductive performance of later laying hens comprises the following components in parts by mass: 2.5 parts of radix fici simplicissimae, 2.5 parts of radix polygonati officinalis, 2 parts of radix paeoniae rubra, 2 parts of folium chenopodii and 1 part of bergenia purpurascens.
The traditional Chinese medicine composition is prepared by the following steps:
weighing the components in parts by mass, and mixing to prepare a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution; wherein the volume mass ratio of the extraction solvent (water) to the traditional Chinese medicine mixture is 60;
spray drying the extractive solution to obtain the Chinese medicinal extract, wherein the air inlet temperature of spray drying is 125 deg.C, the sample injection speed is 7mL/min, and the air inlet amount is 20m 3 In this condition, the water content of the extract was (1.67. + -. 0.09)%, the dissolution time was 30s, the polysaccharide content was (100.21. + -. 5.05) mg/g, and the product yield was (20.38. + -. 1.11)% (1 g corresponds to 5g of crude drug).
Example 2
A traditional Chinese medicine composition for improving reproductive performance of later laying hens comprises the following components in parts by mass: 2 parts of radix fici simplicissimae, 3 parts of radix polygonati officinalis, 1.5 parts of radix paeoniae rubra, 2.5 parts of chenopodium hybridum and 0.5 part of bergenia purpurascens.
The traditional Chinese medicine composition is prepared by the following steps:
weighing the components in parts by mass, and mixing to prepare a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution; wherein the technological parameters of ultrasonic extraction are that the volume mass ratio of the extract to the traditional Chinese medicine mixture is 40; performing ultrasonic treatment for 2 times to obtain extractive solution;
spray drying the extractive solution to obtain the Chinese medicinal extract, wherein the spray drying process parameters are inlet air temperature of 120 deg.C and inlet air volume of 25m 3 The sampling speed of the extracting solution is 5mL/min.
Example 3
A traditional Chinese medicine composition for improving reproductive performance of breeding hens at the later period of egg laying comprises the following components in parts by mass: 3 parts of radix fici simplicissimae, 2 parts of polygonatum odoratum, 2.5 parts of red paeony root, 1.5 parts of Chenopodium Hybridum and 1.5 parts of bergenia purpurascens.
The traditional Chinese medicine composition is prepared by the following steps:
weighing the components in parts by mass, and mixing to prepare a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution; wherein the technological parameters of ultrasonic extraction are that the volume mass ratio of the extract to the traditional Chinese medicine mixture is 80 mL/g, the extraction temperature is 45 ℃, the ultrasonic time is 35min, and the ultrasonic power is 950W; performing ultrasonic treatment for 2 times to obtain extractive solution;
spray drying the extractive solution to obtain the Chinese medicinal extract, wherein the spray drying process parameters are inlet air temperature of 130 deg.C and inlet air volume of 15m 3 The sampling speed of the extracting solution is 10mL/min.
Example 4
A traditional Chinese medicine composition for improving reproductive performance of later laying hens comprises the following components in parts by mass: 2.2 parts of radix fici simplicissimae, 2.4 parts of polygonatum odoratum, 1.8 parts of red paeony root, 1.8 parts of Chenopodium Hybridum and 0.8 part of bergenia purpurascens.
The traditional Chinese medicine composition is prepared by the following steps:
weighing the components in parts by mass, and mixing to prepare a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution; wherein the technological parameters of the ultrasonic extraction are that the volume mass ratio of an extraction solvent (water) to a traditional Chinese medicine mixture is 50 mL/g, the extraction temperature is 48 ℃, the ultrasonic time is 32min, and the ultrasonic power is 980W; performing ultrasonic treatment for 2 times to obtain extractive solution;
spray drying the extractive solution to obtain the Chinese medicinal extract, wherein the spray drying process parameters are inlet air temperature of 128 deg.C and inlet air volume of 18m 3 The sampling speed of the extracting solution is 9mL/min.
Example 5
A traditional Chinese medicine composition for improving reproductive performance of later laying hens comprises the following components in parts by mass: 2.8 parts of radix fici simplicissimae, 2.8 parts of radix polygonati officinalis, 2.3 parts of radix paeoniae rubra, 2.2 parts of chenopodium hybridum and 1.2 parts of bergenia purpurascens.
The traditional Chinese medicine composition is prepared by the following steps:
weighing the components in parts by mass, and mixing to prepare a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixed solvent (water) to obtain an extracting solution; wherein the technological parameters of ultrasonic extraction are that the volume mass ratio of the extract to the traditional Chinese medicine mixture is 70 mL/g, the extraction temperature is 52 ℃, the ultrasonic time is 28min, and the ultrasonic power is 1020W; performing ultrasonic treatment for 2 times to obtain extractive solution;
spray drying the extractive solution to obtain the Chinese medicinal extract, wherein the spray drying process parameters are air inlet temperature of 122 deg.C and air inlet volume of 22m 3 H, the sampling speed of the extracting solution is 6mL/min.
Comparative example 1
A basal feed is used.
Comparative example 2
2.5 parts of radix fici simplicissimae, 2.5 parts of radix polygonati officinalis, 2 parts of radix paeoniae rubra, 2 parts of chenopodium hybridum and 1 part of bergenia purpurascens are mixed and crushed into fine powder which is sieved by a sieve of 80 meshes, and the traditional Chinese medicine powder is prepared.
Comparative example 3
The traditional Chinese medicine powder is prepared by mixing and crushing 2 parts of radix fici simplicissimae, 2 parts of radix polygonati officinalis, 1 part of rhizoma atractylodis macrocephalae, 1 part of radix isatidis and 1 part of liquorice into fine powder which is sieved by a sieve of 80 meshes.
The technical effects of the invention are illustrated below with reference to specific experimental data:
1 Material
1.1 test site and time
The experiments were conducted at a poultry farm at Sichuan university of agriculture.
1.2 test animals
800 400-day-old egg-laying Tianfu broiler breeder chickens.
1.3 test drugs and reagents
The traditional Chinese medicine extract prepared in example 1 and the traditional Chinese medicine powder prepared in comparative examples 2 and 3. A total antioxidant capacity (T-AOC) kit, a total superoxide dismutase (T-SOD) kit, a Malondialdehyde (MDA) kit and a trace reduced Glutathione (GSH) kit are provided by Nanjing-built biotechnology company.
1.4 test devices
Spectrophotometer, electronic scale, platform scale, incubator, etc.
2 test method
2.1 grouping and handling of test animals
800 400-day-old egg-laying Tianfu broiler breeders are divided into 4 groups according to a blank group, a traditional Chinese medicine extract, a traditional Chinese medicine formula 1 group and a traditional Chinese medicine formula 2 group, the blank group is fed with the basic feed in the comparative example 1, the traditional Chinese medicine formula 1 group and the traditional Chinese medicine formula 2 group are respectively fed with the mixture of the traditional Chinese medicine powder prepared in the comparative examples 2 and 3 added with the basic feed according to 5g/kg, the traditional Chinese medicine extract is fed with the mixture of the traditional Chinese medicine composition prepared in the example 1 added with the basic feed according to 1g/kg, each group comprises 200 breeders, each test group is provided with 4 repetitions, and each repetition group comprises 50 Tianfu broiler breeders. And meanwhile, after two hours of feeding each time, the excess material condition is observed, and the excess material is supplemented and recorded in time. The number of dead elutriations was recorded daily and weighed.
Eggs were picked up at 15.
2.2 test animal feeding management
The test chickens are all raised in a standard henhouse, the raising management, disease prevention, health care, sanitation and management of the test chickens are consistent with those of the henhouse according to the conventional method of the feeding farm, the test groups are dosed according to each dosage group, and other treatment is the same as that of the blank group. After continuously feeding for 28 days, the chicken is sacrificed, dissected and blood is collected, sampled and stored for testing.
2.3 detection of indicators
2.3.1 general clinical observations
The mental state, drinking water, behavior activities and feces of each group of test chickens are observed during the test period, and the death and morbidity of the test chickens are recorded.
2.3.2 Productivity
During dosing, 14:00 pick up the eggs and record the weight of the eggs. The trough was cleaned every 7d, the feed consumption weight per repetition of the week was recorded, and the feed-egg ratio was calculated and recorded.
2.3.3 hatching index
And selecting 200 hatching eggs of 28d each group for hatching, recording the fertility rate, the hatching rate, the healthy chick rate and the chick weight for comparative analysis.
2.3.4 Biochemical index examination of blood
After administration, each group selected 12 chicken jugular veins to take blood, serum was separated, and serum biochemical indicators such as total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), malondialdehyde (MDA), and trace reduced Glutathione (GSH) were tested.
2.3.5 visceral indices
After the administration, 12 chickens were collected from each group, sacrificed and weighed after the jugular vein blood sampling, dissected and taken the tissues and organs such as oviduct, liver, follicle, abdominal fat, etc., weighed, and the corresponding organ index was calculated. Organ index = fresh organ weight (g)/live body weight (kg).
2.3.6 data analysis
The test data were statistically processed with the software SPSS21.0, and the results were expressed as mean ± Standard Deviation (SD), with P <0.05 indicating statistical differences.
3 results and analysis
3.1 Effect of Chinese herbal extracts on production Performance of Tianfu broiler breeder chickens
The influence of the traditional Chinese medicine extract added into the feed on the laying rate, the egg weight, the feed-egg ratio and the daily weight gain of Tianfu meat breeders in the later period of egg laying is shown in table 1. The initial weights of the 4 experimental groups were similar, with no significant difference (P > 0.05), and the feeding environment was the same. After the 28d feeding is finished, the egg weight has no significant difference (P > 0.05). Laying rate example 1 group was significantly higher than the other groups (P < 0.05). Compared with the material weight ratio, the group of the example 1 is also obviously lower than the other 3 groups (P < 0.05), and the group of the comparative example 1 has the highest material-egg ratio.
TABLE 1 influence of daily feeding of Chinese herbal extracts on the productivity of Tianfu broiler breeders
Note: in the same row, the difference of the same letter is not significant P >0.05, and the difference of different letters is significant P <0.05, the same below.
3.2 Effect of Chinese herbal medicine extract on hatching Performance of Tianfu broiler breeders
The effect of the daily feeding of herbal extracts on the hatching performance of Tianfu broiler breeders is shown in table 2. Weight of 1 day old chicks example 1 group was significantly higher than the other groups (P < 0.05), and the weight of chicks in comparative examples 2 and 3 was significantly higher than that in comparative example 1 (P < 0.05). While the hatchability of the comparative example 1 group was significantly higher than that of the other groups (P < 0.05). The healthy chick rate is compared, and the group of the example 1 is obviously higher than the group of the comparative example 1 (P < 0.05). Dead embryo rate the control 1 group was more significant than the other groups (P < 0.05).
TABLE 2 Effect of daily feeding of herbal extracts on the hatching behavior of Tianfu broiler breeders
3.3 Effect of Chinese medicinal extracts on serum biochemical indexes of Tianfu broiler breeder
The effect of the traditional Chinese medicine extract added into the daily ration on the biochemical indexes of the blood serum of the Tianfu broiler breeder is shown in table 3. There was no significant difference in MDA content in serum of each test group (P > 0.05), and GSH content of example 1 group was significantly higher than that of other groups (P < 0.05). The GSH content of the comparative example 1 group was significantly lower than that of the comparative examples 2 and 3 and the traditional Chinese medicine extract group (P < 0.05), the T-AOC content of the example 1 group was significantly higher than that of the comparative example 1 group (P < 0.05), and was significantly different from that of the comparative examples 2 and 3 groups (P < 0.05). Compared with the T-SOD content, the content of the group of the comparative example 1 is lower than that of the other three groups, and the difference between the group of the comparative example 1 and the group of the comparative example 2 and the group of the comparative example 3 is not significant (P > 0.05), but the significance is lower than that of the group of the example 1 (P < 0.05).
TABLE 3 influence of the extract of the traditional Chinese medicine fed daily on the biochemical indexes of Tianfu broiler breeder serum
3.4 Effect of Chinese medicinal extracts on organ index of Tianfu broiler breeder
The effect of the addition of the herbal extracts to the daily ration on the organ index of the Tianfu broiler breeder is shown in table 4. The oviduct index of the administration group is significantly higher than that of the comparative example 1 (P < 0.05). The liver index of the comparative examples 2 and 3 and example 1 groups was lower than that of the comparative example 1 group (P < 0.05). Follicle index comparative example 1 group was more significant than comparative example 1 group (P < 0.05). The comparison of the abdominal fat ratios shows that the comparative examples 2 and 3 and the example 1 group are less significant than the comparative example 1 group (P < 0.05).
TABLE 4 Effect of daily feeding of herbal extracts on organ index of Tianfu broiler breeder
4 conclusion
The traditional Chinese medicine extract is added in the daily feed, has the effect of promoting the laying rate and the feed-egg ratio of Tianfu broiler breeders in the later period of egg laying, can also improve the hatching performance, and has the effect of enhancing the serum antioxidant index.
As used in the specification and claims, certain terms are used to refer to particular components or methods. As one skilled in the art will appreciate, different parts may be referred to by different names. The description and claims do not intend to distinguish between components that differ in name but not in name. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. "substantially" means within an acceptable error range, and a person skilled in the art can solve the technical problem within a certain error range to substantially achieve the technical effect. The description which follows is a preferred embodiment of the present application, but is made for the purpose of illustrating the general principles of the application and not for the purpose of limiting the scope of the application. The scope of the present application is to be considered as defined by the appended claims.
It is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a good or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such good or system. Without further limitation, an element defined by the phrases "comprising one of \8230;" does not exclude the presence of additional like elements in an article or system comprising the element.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. The traditional Chinese medicine composition for improving the reproductive performance of the later laying period of the breeding hens is characterized by being prepared from the following components in parts by mass: 2-3 parts of radix fici simplicissimae, 2-3 parts of radix polygonati officinalis, 1.5-2.5 parts of radix paeoniae rubra, 1.5-2.5 parts of chenopodium hybridum and 0.5-1.5 parts of bergenia purpurascens.
2. The traditional Chinese medicine composition according to claim 1, which is prepared from the following components in parts by mass: 2.5 parts of radix fici simplicissimae, 2.5 parts of radix polygonati officinalis, 2 parts of radix paeoniae rubra, 2 parts of folium chenopodii and 1 part of bergenia purpurascens.
3. A traditional Chinese medicine extract for improving the reproductive performance of later-stage laying hens is characterized in that the traditional Chinese medicine extract is extracted from the traditional Chinese medicine composition of any one of claims 1-2.
4. A preparation method of a traditional Chinese medicine extract for improving reproductive performance of breeding hens at the later period of egg laying is characterized by comprising the following steps:
mixing 3-2 parts of Ficus Simplicissima lour, 3-2 parts of radix polygonati officinalis, 2.5-1.5 parts of radix paeoniae rubra, 2.5-1.5 parts of Chenopodium hybridum and 1.5-0.5 part of bergenia purpurascens according to a mass ratio to form a traditional Chinese medicine mixture;
carrying out ultrasonic extraction on the traditional Chinese medicine mixture to obtain an extracting solution;
and carrying out spray drying on the extracting solution to obtain the traditional Chinese medicine extract.
5. The preparation method of the traditional Chinese medicine extract as claimed in claim 4, wherein the technological parameters of the ultrasonic extraction are that the volume mass ratio of the extraction solvent to the traditional Chinese medicine mixture is 40-80.
6. The method for preparing Chinese medicinal extract according to claim 5, wherein the extraction solvent is water.
7. The method for preparing Chinese medicinal extract according to claim 5, wherein the ultrasonic extraction is performed twice on the Chinese medicinal mixture by using ultrasonic waves.
8. The method for preparing a traditional Chinese medicine extract according to claim 4, wherein the spray drying process parameters are that the air inlet temperature is 120-130 ℃, the air inlet amount is 15-25m for carrying out dry distillation/h, and the sample injection speed of the extracting solution is 5-10mL/min.
9. The method for preparing a herbal extract according to claim 4, wherein 2.5 parts of Ficus Simplicissima lour, 2.5 parts of Polygonatum odoratum, 2 parts of radix Paeoniae Rubra, 2 parts of Chenopodium Hybridum and 1 part of bergenia purpurascens are mixed in a mass ratio to form a herbal mixture.
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