KR20200075851A - 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합 - Google Patents

개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합 Download PDF

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KR20200075851A
KR20200075851A KR1020207014099A KR20207014099A KR20200075851A KR 20200075851 A KR20200075851 A KR 20200075851A KR 1020207014099 A KR1020207014099 A KR 1020207014099A KR 20207014099 A KR20207014099 A KR 20207014099A KR 20200075851 A KR20200075851 A KR 20200075851A
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cells
leu
val
ala
gln
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Korean (ko)
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브라이언 버설
필리프 두샤토
알렉산드르 주일러
로랑 프와로
줄리앙 발톤
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셀렉티스
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Priority claimed from PCT/EP2017/076798 external-priority patent/WO2018073391A1/en
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KR1020207014099A 2017-10-19 2018-03-09 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합 Ceased KR20200075851A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247043433A KR20250007705A (ko) 2017-10-19 2018-03-09 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/EP2017/076798 WO2018073391A1 (en) 2016-10-19 2017-10-19 Targeted gene insertion for improved immune cells therapy
EPPCT/EP2017/076798 2017-10-19
EP2018053343 2018-02-09
EPPCT/EP2018/053343 2018-02-09
PCT/EP2018/055957 WO2019076486A1 (en) 2017-10-19 2018-03-09 TARGETED GENE INTEGRATION OF NK INHIBITOR GENES FOR ENHANCED IMMUNE CELL THERAPY

Related Child Applications (1)

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KR1020247043433A Division KR20250007705A (ko) 2017-10-19 2018-03-09 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합

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KR20200075851A true KR20200075851A (ko) 2020-06-26

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KR1020247043433A Pending KR20250007705A (ko) 2017-10-19 2018-03-09 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합
KR1020207014099A Ceased KR20200075851A (ko) 2017-10-19 2018-03-09 개선된 면역 세포들 치료를 위한 nk 억제제들의 타겟인 유전자 통합

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US (2) US12209125B2 (enExample)
EP (2) EP3684919A1 (enExample)
JP (2) JP7621795B2 (enExample)
KR (2) KR20250007705A (enExample)
CN (1) CN111511903B (enExample)
AU (2) AU2018353112B2 (enExample)
CA (1) CA3084476A1 (enExample)
IL (1) IL273828B2 (enExample)
MX (1) MX2020004444A (enExample)
WO (2) WO2019076486A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018189360A1 (en) * 2017-04-13 2018-10-18 Cellectis New sequence specific reagents targeting ccr5 in primary hematopoietic cells
WO2019025800A1 (en) * 2017-08-02 2019-02-07 Autolus Limited CELLS EXPRESSING A CHIMERIC ANTIGENIC RECEPTOR OR A MANIPULATED TCR AND COMPRISING A SELECTIVELY EXPRESSED SELECTIVE NUCLEOTIDE SEQUENCE
WO2019076486A1 (en) 2017-10-19 2019-04-25 Cellectis TARGETED GENE INTEGRATION OF NK INHIBITOR GENES FOR ENHANCED IMMUNE CELL THERAPY
JP2021525530A (ja) 2018-06-01 2021-09-27 ワシントン・ユニバーシティＷａｓｈｉｎｇｔｏｎ Ｕｎｉｖｅｒｓｉｔｙ キメラ抗原受容体細胞治療薬を用いたサイトカイン放出症候群の抑制
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
CN110055224B (zh) * 2019-04-03 2023-06-30 深圳市体内生物医药科技有限公司 一种基因修饰的免疫细胞及其制备方法和应用
JP2022531185A (ja) 2019-04-30 2022-07-06 クリスパー セラピューティクス アクチェンゲゼルシャフト 遺伝子改変ｔ細胞を標的化するｃｄ１９を使用するｂ細胞悪性病変の同種細胞療法
MX2021013223A (es) 2019-05-01 2022-02-17 Juno Therapeutics Inc Celulas que expresan un receptor quimerico de un locus cd247 modificado, polinucleotidos relacionados y metodos.
PH12021553254A1 (en) * 2019-06-27 2022-09-19 Crispr Therapeutics Ag Use of chimeric antigen receptor t cells and nk cell inhibitors for treating cancer
EP4025224A1 (en) 2019-09-05 2022-07-13 CRISPR Therapeutics AG Universal donor cells
JP7753197B2 (ja) * 2019-09-05 2025-10-14 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
EP4031654A4 (en) * 2019-09-20 2023-11-22 The University of North Carolina at Chapel Hill MODIFIED T CELLS AND METHOD FOR PRODUCING THEREOF
IL294388A (en) 2020-01-14 2022-08-01 Synthekine Inc Il2 orthologs and methods of use
AU2021288224A1 (en) * 2020-06-11 2023-01-05 Novartis Ag ZBTB32 inhibitors and uses thereof
WO2021263075A1 (en) * 2020-06-26 2021-12-30 St. Jude Children's Research Hospital, Inc. Methods of generating an activation inducible expression system in immune cells
EP3943097A1 (en) 2020-07-24 2022-01-26 AB2 Bio SA Car-t cell therapy
WO2022020860A2 (en) * 2020-07-24 2022-01-27 Board Of Regents, The University Of Texas System Engineered t cells and tumor-infiltrating lymphocytes to increase tumor killing
WO2022112596A1 (en) 2020-11-30 2022-06-02 Cellectis Sa Use of aminoquinoline compounds for higher gene integration
WO2022143783A1 (en) * 2020-12-29 2022-07-07 Edigene Therapeutics (Beijing) Inc. Methods of identifying t-cell modulating genes
KR20230146007A (ko) 2020-12-31 2023-10-18 크리스퍼 테라퓨틱스 아게 범용 공여자 세포
KR20240007179A (ko) * 2021-04-30 2024-01-16 셀렉티스 에스.에이. 고형 종양 암 면역 요법을 위한 새로운 항-muc1 car 및 유전자 편집된 면역세포
MX2023015153A (es) * 2021-07-01 2024-04-16 Ningbo T Maximum Biopharmaceuticals Co Ltd Polipéptido de unión a antígeno que se dirige a b7h3 y aplicación del mismo.
WO2023016514A1 (zh) * 2021-08-12 2023-02-16 苏州克睿基因生物科技有限公司 经修饰的细胞、其制备方法及应用
WO2023212566A1 (en) * 2022-04-25 2023-11-02 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for preventing t cell exhaustion
CN115820697B (zh) * 2022-09-23 2024-07-30 中国海洋大学 一种免疫细胞及其制备方法和应用
AU2024259472A1 (en) * 2023-04-20 2025-11-13 Peter Maccallum Cancer Institute Compositions and methods for immunotherapy - ii
WO2024258835A1 (en) * 2023-06-16 2024-12-19 The Trustees Of Columbia University In The City Of New York Removal of endogenous tcr chains for enhanced tcr-based immunotherapies
TW202509218A (zh) * 2023-07-12 2025-03-01 亨利傑克森軍方醫學基金會 用於抑制自然殺手細胞之組合物及方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US6534055B1 (en) 1988-11-23 2003-03-18 Genetics Institute, Inc. Methods for selectively stimulating proliferation of T cells
US5858358A (en) 1992-04-07 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Methods for selectively stimulating proliferation of T cells
US6905680B2 (en) 1988-11-23 2005-06-14 Genetics Institute, Inc. Methods of treating HIV infected subjects
US6352694B1 (en) 1994-06-03 2002-03-05 Genetics Institute, Inc. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
WO1994024277A1 (en) 1993-04-13 1994-10-27 Sloan-Kettering Institute For Cancer Research Protection of human bone marrow from high dose antifolate therapy using mutated human dihydrofolate reductase dna
US7175843B2 (en) 1994-06-03 2007-02-13 Genetics Institute, Llc Methods for selectively stimulating proliferation of T cells
US7067318B2 (en) 1995-06-07 2006-06-27 The Regents Of The University Of Michigan Methods for transfecting T cells
US6692964B1 (en) 1995-05-04 2004-02-17 The United States Of America As Represented By The Secretary Of The Navy Methods for transfecting T cells
WO1997033988A1 (en) 1996-03-12 1997-09-18 Sloan-Kettering Institute For Cancer Research Double mutants of dihydrofolate reductase and methods of using same
EP1147209A2 (en) 1999-02-03 2001-10-24 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
HK1047770A1 (zh) 2000-02-24 2003-03-07 Xcyte Therapies, Inc. 细胞的同时刺激与浓缩
US6867041B2 (en) 2000-02-24 2005-03-15 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
US6797514B2 (en) 2000-02-24 2004-09-28 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
JP2006518372A (ja) 2003-01-28 2006-08-10 セレクティス 脊椎動物の体組織においてエクスビボかつイントトで相同的組換えを誘発するためのメガヌクレアーゼの使用およびその応用
EP1620537B1 (en) 2003-03-14 2012-10-24 Cellectis SA Large volume ex vivo electroporation method
CN106834320B (zh) 2009-12-10 2021-05-25 明尼苏达大学董事会 Tal效应子介导的dna修饰
US20120189621A1 (en) * 2011-01-21 2012-07-26 Yann Dean Combination Therapies and Methods Using Anti-CD3 Modulating Agents and Anti-IL-6 Antagonists
EP2699593B2 (en) * 2011-04-20 2020-11-04 University of Washington Through Its Center for Commercialization Beta-2 microglobulin-deficient cells
HUE064187T2 (hu) 2012-05-25 2024-02-28 Cellectis Eljárás immunterápiára alkalmas allogén és immunszupresszív-rezisztens T-sejt módosítására
US20150283233A1 (en) * 2012-06-15 2015-10-08 Gencia Corporation Compositions and Methods for Enhancing Immune Responses
EP3763810A3 (en) * 2012-10-10 2021-07-14 Sangamo Therapeutics, Inc. T cell modifying compounds and uses thereof
AU2014273490B2 (en) * 2013-05-29 2019-05-09 Cellectis Methods for engineering T cells for immunotherapy by using RNA-guided Cas nuclease system
EP3693384B1 (en) 2014-03-11 2024-01-24 Cellectis Method for generating t-cells compatible for allogenic transplantation
CN107109368A (zh) * 2014-10-07 2017-08-29 塞勒克提斯公司 用于调节car诱导的免疫细胞活性的方法
BR112017018224A2 (pt) * 2015-03-11 2018-04-17 Cellectis métodos para manipulação de célula t alogênica para aumentar sua persistência e/ou implante em pacientes
WO2016164492A2 (en) * 2015-04-06 2016-10-13 Regeneron Pharmaceuticals, Inc. Humanized t cell mediated immune responses in non-human animals
AU2016261600B2 (en) 2015-05-08 2021-09-23 President And Fellows Of Harvard College Universal donor stem cells and related methods
IL257105B (en) * 2015-07-31 2022-09-01 Univ Minnesota Modified cells and methods of therapy
US11091780B2 (en) * 2015-09-18 2021-08-17 The Regents Of The University Of California Methods for autocatalytic genome editing and neutralizing autocatalytic genome editing and compositions thereof
CN115806940A (zh) 2015-11-04 2023-03-17 菲特治疗公司 多能细胞的基因组工程改造
EP4012415A3 (en) 2015-12-04 2022-12-07 Juno Therapeutics, Inc. Methods and compositions related to toxicity associated with cell therapy
TW201740958A (zh) * 2016-01-15 2017-12-01 依圖比克斯公司 用於t細胞免疫療法之方法及組合物
AU2017336094A1 (en) * 2016-09-29 2019-04-18 Immunitybio, Inc. HLA class I-deficient NK-92 cells with decreased immunogenicity
CN106636090B (zh) 2016-10-11 2019-08-09 上海优卡迪生物医药科技有限公司 人源白细胞介素6的siRNA、重组表达CAR-T载体及其构建方法和应用
CN108148863B (zh) * 2016-12-05 2019-12-17 上海优卡迪生物医药科技有限公司 一种封闭il6r的用于缓解crs的car-t转基因载体及其构建方法和应用
WO2019076486A1 (en) 2017-10-19 2019-04-25 Cellectis TARGETED GENE INTEGRATION OF NK INHIBITOR GENES FOR ENHANCED IMMUNE CELL THERAPY

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