KR20200064226A - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts Of Ganoderma lucidum, Arnica montana, Buddleja And Thymus vulgaris As Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of Ganoderma lucidum, Arnica montana, Buddleja davidii, and Thymus vulgaris as an active component. More specifically, the present invention relates to a cosmetic composition for moisturizing skin having excellent effects of skin moisturizing and skin barrier improvement.

Description

Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts Of Ganoderma lucidum, Arnica montana, Buddleja And Thymus vulgaris As Active Ingredient}

The present invention relates to a cosmetic composition containing as an active ingredient a Youngji mushroom, Arnica montana flower, budleya and thyme mixed extract, and more particularly, to a moisturizing cosmetic composition.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of naturally occurring skin with age. In general, aging is due to oxidative damage of biomaterials by free radicals. That is, free radicals with high reactivity such as free radicals such as hydrogen peroxide, hydroxy radicals, and superoxide radicals peroxidize lipids constituting the cell membrane, destroying the cell membrane, oxidizing proteins, attacking hexane, and causing oxidative damage. Destroy the cells and eventually kill the cells. The most significant skin characteristics due to aging include wrinkles, decreased elasticity, and dull skin.

In addition, in addition to wrinkles and reduced elasticity, after the 40s, the face color is often unclear and gray and dark. In particular, in the case of young women, as they experience childbirth, blood bleeding occurs, and blood circulation disorders occur, resulting in a decrease in metabolic ability and skin roughening and dullness. When the metabolic ability decreases and the skin becomes rough, the amount of moisture is also easily reduced. In general, the amount of moisture in the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, resulting in about 10 to 30% in the keratinocyte layer. It is known that in the keratinocyte layer, a substance having a hydrophilic water retention ability called a natural moisturizing factor (NMF) exists and plays an important role in moisturizing the skin. In the normal keratinocyte layer, when the moisture is maintained at about 10 to 30%, the skin is smooth and soft, and the body protects normally.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin to prevent skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea are electrolytic. This strong, there is a problem that impairs the emulsion stability of the cosmetic. Amino acids, collagen, and elastin also have moisturizing abilities, but have limited moisturizing abilities. The ability to retain moisture in the stratum corneum can be controlled by natural moisturizing factors (NMF) composed of sebum components, amino acids, lactic acid, urea, and inorganic salts. It is safe and excellent in use as a natural moisturizing factor in external preparations for skin. In addition, the development of substances with high moisturizing effects is one of the important research tasks in cosmetics. In addition, substances known to have antioxidant activity include ascorbic acid, dl-α-tocopherol, beta-carotene, dibutylhydroxytoluene (BHT), etc. Since these materials are used as synthetic products, there is an urgent need for the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully because they can cause skin safety problems when used for a long time or when used in large quantities.

Therefore, research on improving skin aging and moisturization is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and recently, as the consumer's response to cosmetics using natural materials has increased, the development value of cosmetics has increased.

To this end, research is being actively conducted to search for the skin physiological activity of various natural plant components and to develop substances having excellent safety and excellent skin beauty effects.

Korean Patent Publication No. 1996-016864 discloses a pack composition containing Youngji powder.

Accordingly, the present inventors studied the applicability of cosmetics to various natural ingredients, which have not been known, as a result, selected Youngji mushroom, Arnica montana flower, Budella, and thyme, and prepared an extract therefrom to measure the moisturizing effect. , It was discovered that the efficacy as a cosmetic can be expected since its efficacy is very excellent.

An object of the present invention is to provide a cosmetic composition for skin protection that exhibits a moisturizing effect by containing a mixture of mandarin mushroom, arnica montana flower, budleya and thyme.

In addition, an object of the present invention is to provide a cosmetic composition showing supercritical, ultrasonic, and fermented extracts and efficacious effects through a purification process in order to maximize the efficacy of the ganoderma mushroom, arnica montana flower, budleya and thyme mixed extract .

The present invention relates to a cosmetic composition for moisturizing the skin containing a young manji mushroom, Arnica montana flower, budleya and thyme mixed extract.

Preferably, the cosmetic composition has an effect of improving skin barrier function.

In addition, preferably, the reishi mushroom, arnica montana flower, budleya and thyme mixed extract may be contained in an amount of 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition.

In addition, preferably, the young mango mushroom, Arnica montana flower, willley and thyme mixed extract (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butyl Selected from the group consisting of a solvent extraction method, (b) supercritical extraction, (c) ultrasonic extraction, and (d) fermentation extraction using microorganisms, using one or more solvents selected from the group consisting of renglycol, hexane, and mixtures thereof. It is characterized by being extracted by an extraction method.

In addition, preferably, the ganoderma mushroom, arnica montana flower, budleya and thyme mixed extract is contained in a mixed weight ratio of 1 to 3: 1 to 3: 1 to 3: 1 to 3, which is a cosmetic composition for moisturizing the skin. It is characterized by.

Also preferably, the cosmetic composition is a group consisting of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It may have any one formulation selected from.

The Youngji mushroom, Arnica montana flower, Boudella and thyme extract according to the present invention is excellent in skin moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless defined otherwise, have the following definitions and conform to the meaning as commonly understood by those skilled in the art in the relevant field of the present invention. Also, a preferred method or sample is described herein, but similar or equivalent ones are included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, for reference amount, level, value, number, frequency, percent, dimension, size, amount, weight or length It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, the terms “comprises” and “comprising”, unless the context requires otherwise, include the steps or components presented, or groups of steps or components, but any other steps or components, or It should be understood that it implies that a group of steps or components is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin containing a young manji mushroom, Arnica montana flower, budleya and thyme mixed extract.

Youngji mushroom (Ganoderma lucidum), which is used as an active ingredient in the cosmetic composition of the present invention, grows in a living tree or dead tree stump of hardwoods from early summer to autumn. Yeongji mushrooms are called yeongji because they are called medicinal herbs in China, and ginseng is glossy, initially yellowish-white, and later red or purple. Glucan, a type of polysaccharide contained in reishi mushroom, helps to enhance skin immunity. Youngji mushroom of the present invention uses outpost among various organs or parts (eg, leaves, bark, branches, flowers, roots, fruits, stems, etc.) of the manji mushroom.

Arnica montana flowers grow on highland grasses and are 20-30cm high. The leaves come out of the roots and spread all over the stems, facing the stems. Branches are split at the end, and 1 to 3 duhwa runs. The flowers bloom yellow in June-July, 6-8cm in diameter, and have thick hairs and hairs overall. Flowers and rhizomes have a bitter taste and contain essential oils and resins. In European civilians, flowers and rhizomes have been used as a universal medicine since ancient times. It is used as a hemostatic agent for stimulation of angina, vasodilation, relief of vasospasm, bruises and hemorrhoids in oriental medicine. The Arnica montana flower of the present invention uses outpost among various organs or parts (eg, leaves, bark, branches, flowers, roots, fruits, stems, etc.) of Arnica montana flowers.

Buddleja is a deciduous shrub that is about 1 to 3 m high and has white star-shaped fluffy hairs on young branches, on the back of leaves, and on the ear of flowers. The small branches are square, the leaves are opposite, and there are serrate edges on the edge. Flowers bloom in July-September, light purple, and yellow flowers at the rear end are densely inflorescences, and the whole becomes conical inflorescences. Calyx is divided into four. The buddleia of the present invention uses outpost among the various organs or parts of buddleia (eg, leaves, bark, branches, flowers, roots, fruits, stems, etc.).

The thyme (Thymus vulgaris) is native to southern Europe. It is 20cm high and has white hairs on its branches. The leaves are opposite, egg-shaped, oval or string-shaped, 4-15mm long, and the edges are dried back. Flowers bloom purple in summer and run in multiple layers. It is similar to Korean thyme, but it is different from standing upright and has hair on the leaves. In Europe, it is used as a flavor for sauces and tomato ketchup, and boiled as a cough medicine. The thyme of the present invention uses outpost among various organs or parts of thyme (eg leaf, bark, branch, flower, root, fruit, stem, etc.).

In the present invention, the mandarin mushroom, arnica montana flower, budleya and thyme mixed extract means obtained by extracting from various organs or parts of the manor mushroom, arnica montana flower, budleya and thyme.

In addition, the mandarin mushroom, arnica montana flower, budleya and thyme mixed extract can be prepared according to conventional methods known in the art. For example, extraction is performed using (a) a solvent extraction method, (b) a supercritical extraction method with reduced pressure and high temperature with carbon dioxide, (c) an ultrasonic extraction method, or (d) a fermentation extraction method.

In the present invention, the mandarin mushroom, arnica montana flower, budleya and thyme mixed extracts are various extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol) , Using one or more extraction solvents selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids and mixtures thereof. Can be obtained, preferably obtained using ethanol, 70% (v/v) ethanol or water, most preferably obtained using 70% (v/v) ethanol.

On the other hand, it is apparent to those skilled in the art that the extract of the mandarin mushroom, arnica montana flower, budleya and thyme extract of the present invention can obtain an extract exhibiting substantially the same effect using not only the above-described extraction solvent, but also other extraction solvents. will be.

In addition, the Youngji mushroom, arnica montana flower, budleya and thyme extract of the present invention include not only extracts by the above-described extraction solvent, but also extracts that have been subjected to conventional purification and fermentation processes. For example, reduced pressure by carbon dioxide, extraction by supercritical extraction method by high temperature, extraction by ultrasonic extraction method, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) Active fractions obtained through various purification and extraction methods additionally carried out, such as extraction by fermentation products using natural or various microorganisms, or separated by a product prepared for separation according to sex) are also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cold filtration at room temperature, filtered by heating, and further obtained by concentrating or freeze-drying the solvent under reduced pressure.

Decompression by carbon dioxide and extraction by supercritical extraction by high temperature mean supercritical fluid extraction. Generally, supercritical fluid is a liquid that a gas has when it reaches a critical point under high temperature and high pressure conditions, and It has the properties of a gas and has a chemically similar polarity to a non-polar solvent, and due to these properties, supercritical fluids are used for the extraction of fat-soluble materials (J. Chromatogr. A. 1998; 479:200-205).

Carbon dioxide becomes a supercritical fluid having both liquid and gaseous properties through the process of pressure and temperature reaching a critical point through the operation of a supercritical fluid device, and as a result, solubility in fat-soluble solute increases. When supercritical carbon dioxide passes through an extraction container containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from supercritical carbon dioxide.

After extracting the fat-soluble substance, passing the supercritical carbon dioxide containing a small amount of co-solvent again through the sample remaining in the extraction container may allow components not extracted with pure supercritical carbon dioxide to be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a co-solvent is additionally mixed with carbon dioxide.

The co-solvent may use one or two or more mixtures selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acids and diethyl ether. Preferably, one or two or more mixtures selected from the group consisting of olive oil, ethanol, methanol and water can be used.

Most of the extracted sample contains carbon dioxide, and since carbon dioxide volatilizes into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the co-solvent can be removed by a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method that uses energy generated by ultrasonic vibration, and ultrasonic waves can destroy insoluble solvents contained in a sample in a water-soluble solvent, and reactants located around due to the high local temperature generated at this time. Since the kinetic energy of the particles is increased, sufficient energy necessary for the reaction is obtained, and high pressure is induced by the impact of ultrasonic energy to increase the mixing effect of the substance and the solvent contained in the sample, thereby increasing extraction efficiency.

As an extraction solvent that can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether can be used. The extracted sample is vacuum filtered to recover the filtrate, and then removed with a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze drying.

Youngji mushroom, arnica montana flower, budleya and thyme mixed extract according to the present invention also includes an extract that has undergone a fermentation process. Youngji mushroom, arnica montana flower, budaleya and thyme fermentation extract can be prepared as follows. Ginseng mushroom, Arnica montana flower, budleya, and thyme are finely crushed to about 100-500 mesh, and then 1-50 g/L of a normal microbial culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria of 10,000-100,000 cfu/L. Add in quantity. The culture temperature is cultured under normal microbial culture conditions of 30~37℃. The pH is 5 to 7 and cultured for about 5 to 10 days under aerobic or normal anaerobic conditions. It can then be obtained through aging and filtration.

According to the present invention, the mandarin mushroom, Arnica montana flower, budleya and thyme mixed extract is contained 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition, preferably, 0.01 to 20% by weight, more preferably It is characterized in that it contains 0.1 to 10% by weight. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect on the increase in content is negligible, and there is a problem in the safety and stability of the formulation. Not even enemies

Youngji mushroom, arnica montana flower, budleya and thyme extract according to the present invention contains young mango mushroom, arnica montana flower, budleya and thyme in a mixed weight ratio of 1~3:1~3:1~3:1~3 It may be, and preferably may be included in a mixed weight ratio of 1:1:1:1.

After using the refining process to remove the reishi mushroom, arnica montana flower, budleya, and thyme mixed extracts prepared by a conventional method in a cosmetic formulation, it may include a cosmetic composition exhibiting an efficacy effect. The purification process may be any conventional purification process, and a liquid-liquid extraction method using a difference in solubility, a precipitation and a filter, a chromatography method using a size of polarity and molecular weight, or the like may be used. In addition, for example, after dissolving a mixture of mandarin mushroom, arnica montana flower, budleya and thyme in an ethanol aqueous solution (50%, 70% solution, etc.) or other aqueous solution, use a column filled with HP-20, sephadex column A water-soluble substance and an oil-soluble substance may be separated using a difference in polarity and molecular weight, and the oil-soluble substance may be concentrated and freeze-dried by TLC to prepare a concentrate.

Ingredients included in the cosmetic composition of the present invention, as an active ingredient, may include, in addition to the active ingredient, ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances Adjuvants, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but may be formulated, but is not limited thereto. More specifically, it can be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as a carrier component. Can be.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.

In the case of cleansing in which the formulation of the present invention contains a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acids as carrier components Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be wiped, peeled off, or washed with water after being applied to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, a cleansing towel and a cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in more detail through manufacturing examples. These preparation examples are only for more specifically describing the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Example 1: Youngji Mushroom, Arnica montana flower, Budella and thyme mixed extract

Finely shredded ganoderma mushroom (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed 60g each, and then they are mixed with 70% (v/v) ethanol solution 3 times for 5 hours. After reflux extraction and cooling, it was filtered through Whatman #4 filter paper. The filtered extract was removed using an ultrafiltration membrane and then concentrated under reduced pressure and freeze-dried at 50° C. or less to obtain 6.7 g. Then, the dried manji mushroom, arnica montana flower, budleya and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 2: Supercritical fluid mixed extract of Youngji mushroom, Arnica montana flower, Budella and thyme

After dividing and mixing 60 g each of the shaded Yeongji mushrooms (outposts), Arnica montana flowers (outposts), budleyas (outposts) and thyme (outposts), this is a normal supercritical extraction method (about 300 atmospheres at a temperature of about 60℃). (atm) under supercritical pressure, extracted with olive oil as a co-solvent under supercritical condition), and then removed with a salt using an ultrafiltration membrane, concentrated under reduced pressure and freeze-dried to remove 31.3 g of dried mango mushroom, Arnica montana flower , Puddle and thyme mixed extract powder was obtained. It was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 3: Preparation of ultrasonic mixed extract of Youngji Mushroom, Arnica montana flower, Budella and thyme

Finely shredded ganoderma lucidum (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed by 60g each, and then ultrasonic (with a supernonic ultrasonic device to a intensity of 25 KHz 30 Extraction at about °C for about 2 hours), and salt was removed using an ultrafiltration membrane, and then 7.1g was obtained through concentration under reduced pressure and freeze drying. The dried manor mushroom, Arnica montana flower, Boudella, and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 4: Youngji Mushroom, Arnica montana flower, Budella and thyme mixed extract

Finely shredded ganoderma mushroom (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed with 96g, 48g, 48g, 48g, respectively, and 70% (v/v) ethanol aqueous solution After extracting reflux 3 times for 5 hours and cooling, the filter was filtered with Whatman #4 filter paper. The filtered extract was removed using an ultrafiltration membrane and then concentrated under reduced pressure and freeze-dried at 50° C. or less to obtain 6.7 g. Then, the dried manji mushroom, arnica montana flower, budleya and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 5: Youngji mushroom, Arnica montana flower, Budella and thyme mixed extract

Finely shredded ganoderma mushroom (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed 48g, 96g, 48g, 48g, respectively, and 70% (v/v) ethanol aqueous solution After extracting reflux 3 times for 5 hours and cooling, the filter was filtered with Whatman #4 filter paper. The filtered extract was removed using an ultrafiltration membrane and then concentrated under reduced pressure and freeze-dried at 50° C. or less to obtain 6.8 g. Then, the dried manji mushroom, arnica montana flower, budleya and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 6: Youngji mushroom, Arnica montana flower, Budella and thyme mixed extract

Finely shredded ganoderma mushroom (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed 48g, 48g, 96g, 48g, respectively, and 70% (v/v) ethanol aqueous solution After extracting reflux 3 times for 5 hours and cooling, the filter was filtered with Whatman #4 filter paper. The filtered extract was removed using an ultrafiltration membrane, concentrated under reduced pressure and freeze-dried at 50°C or lower to obtain 7.1g. Then, the dried manji mushroom, arnica montana flower, budleya and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 7: Youngji mushroom, Arnica montana flower, Budella and thyme mixed extract

Finely shredded ginji mushroom (outpost), arnica montana flower (outpost), budleya (outpost) and thyme (outpost) are mixed 48g, 48g, 48g, 96g, respectively, and 70% (v/v) ethanol aqueous solution After extracting reflux 3 times for 5 hours and cooling, the filter was filtered with Whatman #4 filter paper. The filtered extract was removed using an ultrafiltration membrane and then concentrated under reduced pressure and freeze-dried at 50° C. or less to obtain 6.8 g. Then, the dried manji mushroom, arnica montana flower, budleya and thyme mixed extract powder were dissolved in 50% 1,3-butylene glycol and used.

Formulation Example 1 Preparation of Cosmetic Composition Containing Youngji Mushroom, Arnica Montana Flower, Boudella, and Thyme Extract

Cosmetics (Formulation Examples 1 to 7) each comprising extracts of Youngji mushrooms, Arnica montana flowers, Boudella and thyme of Preparation Examples 1 to 7 were prepared as shown in Table 1 below. In addition, for comparison of efficacy, a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizer instead of a mixture of young mushrooms, arnica montana flowers, budleya, and thyme (Comparative Formulation Example 1), both extracts and moisturizers A cosmetic composition that does not contain (Comparative Formulation Example 2) was prepared as shown in Table 1 below.

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Comparative Formulation Example 1 Comparative Formulation Example 2 Preparation Example 1 5.0 - - - - Preparation Example 2 - 5.0 - - - Preparation Example 3 - - 5.0 - - Preparation Example 4 5.0 Preparation Example 5 5.0 Preparation Example 6 5.0 Preparation Example 7 5.0 glycerin - - - 5.0 - 1.3 Butylene glycol - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystal 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Floating paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Proper Proper Proper Proper Proper Proper Proper Proper Proper Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1: Moisturizing effect of Youngji mushroom, Arnica montana flower, Budella, and thyme extract 1-skin electrical conductivity

The clinical moisturizing effect and lasting power for the cosmetic compositions of Formulation Examples 1 to 7 were measured as follows. A group of 90 healthy adult men and women who feel dry skin through surveys was randomly formed into groups of 10, and the formulation examples 1 to 7 and the comparative formulations 1 and 2 for each group were two formulations twice a day for 4 weeks. Was applied to both faces one by one. The moisturizing effect was evaluated by numerically evaluating the increase rate (%) of skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage +Khazaka,Germany). The experimental results are shown in Table 2 below.

division Before use 2 weeks after use 4 weeks after use Formulation Example 1 25.76 40.61 49.52 Formulation Example 2 24.95 39.98 47.05 Formulation Example 3 24.20 41.26 50.30 Formulation Example 4 24.28 36.77 46.48 Formulation Example 5 22.90 37.54 44.25 Formulation Example 6 23.50 34.23 43.13 Formulation Example 7 23.22 24.77 38.89 Comparative Formulation Example 1 25.23 36.37 42.13 Comparative Formulation Example 2 23.19 24.85 26.98

As shown in Table 2, even if compared to a cosmetic containing a chemical moisturizing agent as a comparative formulation (Comparative Formulation Examples 1 and 2), the cosmetic product containing the young mango mushroom, arnica montana flower, budleya and thyme mixed extract of the present invention ( It was confirmed that the moisturizing effect of Formulation Examples 1 to 7 was more excellent. In addition, when the Formulation Example 1 and Formulation Examples 4 to 7 were compared, the efficacy of Formulation Example 1 was excellent, and mixed as a mixing ratio of 1:1:1:1. It was confirmed that the most desirable.

Experimental Example 2: Moisturizing effect of Youngji mushroom, Arnica montana flower, Budella and thyme extract 2-Skin barrier improvement effect

25 healthy women in a room with no air flow at 25°C and relative humidity of 45% were tested. Skin barrier damage was caused by using 1% aqueous solution of sodium lauryl sulfate (SLS), and after applying the cosmetic compositions of formulations 1, 2 and 3 to the upper arm of the arm, TEWAMETER TM210 (C+K eletronic GmhH. Germany) to measure the amount of transdermal moisture loss. In other words, the TEWL (Transepidermal Water Loss) value was measured to measure the change in TEWL value over time to determine the rate of skin barrier recovery (%). The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 52.78 65.98 83.22 96.45 Formulation Example 2 52.16 66.96 88.38 99.55 Formulation Example 3 51.82 66.45 82.73 98.06 Formulation Example 4 45.23 54.22 68.88 78.56 Formulation Example 5 46.52 57.88 70.23 80.14 Formulation Example 6 44.43 57.10 68.22 70.98 Formulation Example 7 38.45 51.44 56.83 68.42 Comparative Formulation Example 1 39.97 50.23 55.64 64.78 Comparative Formulation Example 2 40.55 57.62 63.74 72.74

As shown in Table 3, the formulation examples 1 to 7 containing the manor mushroom, arnica montana flower, budleya and thyme mixed extracts are comparative formulation examples not containing the manor mushroom, arnica montana flower, budleya and thyme mixed extracts It can be seen that transdermal water loss was improved compared to 1 and 2. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 7, the efficacy of Formulation Example 1 was excellent, and it was confirmed that it is most preferable to mix at a mixing ratio of 1:1:1:1.

Experimental Example 3: Safety test of Youngji mushroom, Arnica montana flower, Budella and thyme extract

In order to confirm whether or not the skin irritation to the cosmetic compositions of Formulation Examples 1 to 7 was performed, a human skin patch test was conducted. A comparative formulation that did not show hypersensitivity to skin irritation due to past history and currently has 20 women in their 20s and 30s who have no skin disease or skin allergy symptoms, and does not contain young mango mushrooms, Arnica montana flowers, budleya, and thyme extracts Examples 1 and 2 were used as controls.

First, the test site was wiped off with 70% ethanol and then dried. The prepared test substance was dropped into a pin chamber (Finn chamber, 100X10, EPITEST), and then closed and sealed on the test subject's back. Patching was performed for 24 hours, and after removal of the patch, the presence or absence of erythema and edema of the test site was observed using a magnifying glass (8MC-150, DAZOR) 1 hour and 24 hours later.

Skin reactions were determined according to the rules of the International Contact Dermatitis Research Group (ICDRG) (Table 4), and the average skin reactivity was calculated by Equation 1 below and the results are shown in Table 5.

sign score Evaluation standard - 0 No response ± 0.5 Faint or mild erythema + One Erythema, edema, and papules with clear but weak boundaries ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe erythema and large blisters, peeling

division 24 hours 48 hours Average skin response
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.14 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.14 Formulation Example 4 One - - One - - 0.28 Formulation Example 5 2 - - - - - 0.28 Formulation Example 6 2 - - - - - 0.28 Formulation Example 7 One - - One - - 0.28 Comparative Formulation Example 1 2 - - - - - 0.28 Comparative Formulation Example 2 2 - - - - - 0.28

As shown in Table 5, since the cosmetic compositions of Formulation Examples 1 to 7 do not all cause skin irritation, it can be seen that the safety of the cosmetic formulations to which the Youngji Mushroom, Arnica montana flower, Budella and thyme extracts are added is not abnormal. there was.

Since the specific parts of the present invention have been described in detail above, it is obvious that for those skilled in the art, this specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

A cosmetic composition for moisturizing the skin, containing a mixture extract of mandarin mushroom, arnica montana flower, budleya and thyme. According to claim 1, The cosmetic composition has a skin barrier function improving effect, the skin moisturizing cosmetic composition. The method of claim 1, wherein the ganoderma mushroom, Arnica montana flower, budleya and thyme mixed extract is contained in 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition, cosmetic composition for moisturizing skin. The method of claim 1, wherein the manji mushroom, Arnica montana flower, budleya and thyme mixed extract (a) water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate , Solvent extraction method using an extraction solvent selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, nucleic acids and mixtures thereof, (b) supercritical extraction method and (c) extracted by ultrasonic extraction method, Cosmetic composition for moisturizing the skin. The cosmetic composition for moisturizing the skin according to claim 1, wherein the mandarin mushroom, arnica montana flower, budleya and thyme mixed extract are contained in a mixed weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3 .