KR20200061773A - Method for culturing lactobacillus for improving cognitive ability and lactobacillus cultured by the same - Google Patents

Abstract

Disclosed is a method for culturing lactobacillus for improving memory using spirulina, lactobacillus for improving memory cultivated by the cultivation method, and health functional food containing the lactobacillus. When culturing lactobacillus to the culture medium containing a spirulina powder or a spirulina extract, the amount of lactobacillus to be cultured increases, and functional activity including memory of the cultured lactobacillus is excellent.

Description

Method for culturing lactic acid bacteria for improving memory and lactic acid bacteria cultured by the above culture method {METHOD FOR CULTURING LACTOBACILLUS FOR IMPROVING COGNITIVE ABILITY AND LACTOBACILLUS CULTURED BY THE SAME}

The present invention relates to a method for culturing lactic acid bacteria for improving memory using spirulina, a lactic acid bacteria for improving memory cultivated by the culture method, and a health functional food comprising the lactic acid bacteria.

Prebiotics (prebiotics) is defined as an indigestible food additive that has a beneficial effect on the host by limiting microorganisms in the intestine or selectively promoting the growth of beneficial microorganisms. It contributes to the improvement of health.

Common ingredients of prebiotics are not digested and digested in the stomach or small intestine, but are good foods for beneficial strains in the large intestine, and oligosaccharides and dietary fiber are typical examples. Oligosaccharides may include various types of oligosaccharides, such as fructooligosaccharide, galactooligosaccharide, isomaltoligosaccharide, xyloligosaccharide, and chitin oligosaccharide. In addition, dietary fibers include dextrin, inulin, and natural rubber.

On the other hand, spirulina's prebiotic effect is that it uses abundant dietary fiber present in spirulina, not oligosaccharides, but it is slower than oligosaccharides in the intestine, but is degraded and fed by lactic acid bacteria (derived from kimchi). In particular, due to this prebiotic effect, lactic acid bacteria in the intestine may proliferate, and recently, research on treating mental diseases and improving memory and cognitive ability by controlling communication between the intestinal microbes and the brain. It is actively progressing.

Specifically, it is a general premise that the growth of microbes in the intestine and the improvement of memory or cognitive ability are related to the intestinal nervous system, immune system, vagus nervous system, and hormones and neurotransmitters in the intestine, as well as the bacterial-intestinal-brain connection. It is known that it can improve cognitive function beyond affecting neurological and mental disorders.

Accordingly, the present inventors during the research as described above, when culturing lactic acid bacteria in a culture medium containing spirulina powder or spirulina extract, the amount of lactic acid bacteria cultured increases, and the cultured lactic acid bacteria are found to have excellent functional activity including memory. Thus, the present invention has been completed.

In this regard, Korean Patent Registration No. 10-1087972 discloses a fermentation product of lactic acid bacteria that is effective in preventing/treatment of dementia and dementia-related diseases and improving cognitive dysfunction, and a method and method of manufacturing the same.

The present invention has been devised to solve the aforementioned problems, and an embodiment of the present invention provides a method for culturing lactic acid bacteria for improving memory using spirulina.

In addition, another embodiment of the present invention provides a lactic acid bacteria for improving memory, cultured by the culture method.

In addition, another embodiment of the present invention provides a health functional food containing the lactic acid bacteria.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the following description. There will be.

As a technical means for achieving the above-described technical problem, one aspect of the present invention,

Preparing a lactic acid bacteria culture medium; Sterilizing the culture medium of the lactic acid bacteria; Inoculating the sterile lactic acid bacteria culture medium; And culturing the lactic acid bacteria by adding spirulina powder or spirulina extract to the lactic acid bacteria culture medium inoculated with the lactic acid bacteria; provides a method for culturing lactic acid bacteria for improving memory.

The culture medium of lactic acid bacteria, distilled water, yeast extract, peptone, glucose, magnesium sulfate, manganese sulfate, potassium phosphate, polysorbate 80 (polysorbate 80) and combinations thereof.

The content of the yeast extract compared to 100 parts by weight of the total composition of the lactic acid bacteria culture medium is 0.1 parts by weight to 1 part by weight, the content of peptone is 0.1 parts by weight to 2 parts by weight, and the content of glucose is 1 part by weight to 5 parts by weight, The content of magnesium sulfate is 0.001 part by weight to 1 part by weight, the content of manganese sulfate is 0.001 part by weight to 1 part by weight, the content of potassium phosphate is 0.01 part by weight to 1 part by weight, the content of polysorbate 80 May be 0.001 part by weight to 1 part by weight.

The spirulina may be spirulina platensis or spirulina maxima.

The spirulina extract is derived from the group consisting of water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol and combinations thereof. It may be extracted with a selected solvent.

The added amount of the spirulina powder or spirulina extract may be 1 part by weight to 20 parts by weight relative to 100 parts by weight of the total composition of the lactic acid bacteria culture medium.

The lactic acid bacteria include Lactobacillus plantarum, L. casei, L. acidophilus, Lactobacillus L. bulgaricus, Bifidobacterium B. longum, B. bifidum, B. bifidum, Actiregularis, L. rhamnosus, and lactic acid bacteria selected from the group consisting of combinations thereof May be

The culture may be performed for 6 hours or more.

In addition, another aspect of the present invention,

It provides a lactic acid bacteria for improving memory, cultured by the above culturing method.

In addition, another aspect of the present invention,

It provides a health functional food containing lactic acid bacteria for improving the memory.

According to an embodiment of the present invention, when lactic acid bacteria are cultured in a culture medium containing the spirulina powder or spirulina extract, the amount of lactic acid bacteria to be cultured increases, and there is an excellent effect of functional activity including memory of the cultured lactic acid bacteria.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a view showing a schematic diagram of a perfusion culture device for culturing lactic acid bacteria according to an embodiment of the present invention.
2 is a graph showing cell growth according to the culture time of lactic acid bacteria according to an experimental example of the present invention.
3 is a graph showing the results of measuring the maze activity in water according to an experimental example of the present invention.
Figure 4 is a graph showing the results of measuring the passive avoidance activity according to an experimental example of the present invention.

Hereinafter, the present invention will be described in more detail. However, the present invention may be implemented in various different forms, and the present invention is not limited by the examples described herein, and the present invention is only defined by the claims below.

In addition, the terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. 'Including' a component throughout the specification of the present invention means that other components may be further included instead of excluding other components, unless otherwise stated.

The first aspect of the present application,

Preparing a lactic acid bacteria culture medium; Sterilizing the culture medium of the lactic acid bacteria; Inoculating the sterile lactic acid bacteria culture medium; And culturing the lactic acid bacteria by adding spirulina powder or spirulina extract to the lactic acid bacteria culture medium inoculated with the lactic acid bacteria.

Hereinafter, a method for culturing the lactic acid bacteria for improving memory according to the first aspect of the present application will be described in detail step by step.

First, in one embodiment of the present application, the method for culturing lactic acid bacteria for improving memory includes preparing lactic acid bacteria culture medium.

In one embodiment of the present application, the lactic acid bacteria culture medium is not limited as long as it is a commonly used lactic acid bacteria culture medium, for example, the lactic acid bacteria culture medium is distilled water, yeast extract (yeast extract), peptone (peptone), glucose ( glucose, magnesium sulfate, manganese sulfate, potassium phosphate, polysorbate 80, and combinations thereof. have.

In one embodiment of the present application, the content of the yeast extract relative to 100 parts by weight of the total composition of the lactic acid bacteria culture medium may be 0.1 parts by weight to 1 part by weight, and preferably 0.3 parts by weight to 0.7 parts by weight. The content of the peptone may be 0.1 parts by weight to 2 parts by weight, and preferably 0.5 parts by weight to 1.5 parts by weight. The content of glucose may be 1 part by weight to 5 parts by weight, preferably 1 part by weight to 3 parts by weight. The content of the magnesium sulfate may be 0.001 part by weight to 1 part by weight, preferably 0.005 part by weight to 0.015 part by weight. The content of the manganese sulfate may be 0.001 part by weight to 1 part by weight, preferably 0.001 part by weight to 0.01 part by weight. The content of the potassium phosphate may be 0.01 parts by weight to 1 part by weight, preferably 0.1 parts by weight to 0.5 parts by weight. The content of the polysorbate 80 may be 0.001 part by weight to 1 part by weight, preferably 0.01 part by weight to 0.5 part by weight.

Next, in one embodiment of the present application, the method of culturing the lactic acid bacteria for improving memory includes sterilizing the culture medium of the lactic acid bacteria.

In one embodiment of the present application, sterilization of the culture medium for lactic acid bacteria is not limited as long as it employs a sterilization method that is commonly used, for example, the sterilization may be performed at a temperature of 100° C. or higher for 5 minutes or longer.

Next, in one embodiment of the present application, the method for culturing the lactic acid bacteria for improving memory includes inoculating the sterile lactic acid bacteria in the culture medium.

In one embodiment of the present application, the lactic acid bacteria are Lactobacillus plantarum (L.plantarum), Lactobacillus casei, L. acidophilus, L. acidophilus, Lactobacillus bulgaricus (L .bulgaricus, B. longum, B. bifidum, Actiregularis, L. rhamnosus and combinations thereof It may be to include lactic acid bacteria selected from the group.

In one embodiment of the present application, it may be to incubate the lactic acid bacteria for a predetermined time after inoculating the lactic acid bacteria. This may be performed separately from the step of culturing lactic acid bacteria to be described below, and in the case of this step, the culture may be cultivated at a temperature of about 30°C to about 40°C for about 10 hours to about 30 hours.

Next, in one embodiment of the present application, the method of culturing the lactic acid bacteria for improving memory includes culturing the lactic acid bacteria by adding spirulina powder or spirulina extract to the lactic acid bacteria culture medium inoculated with the lactic acid bacteria.

In one embodiment of the present application, the spirulina has a spiral shape as a blue-green blue-green algae, and a size of 10 μm in width and 300 μm to 500 μm in length can be observed with one naked eye. The word spirulina is synonymous with the word spiral and both are derived from Latin, meaning either twisted or spiral. Spirulina has been known since the 1967 International Conference on Applied Microorganisms, after the announcement of Spirulina native to Lake Arangua near Ethiopia. This new plant is similar to chlorella, has a much higher protein content, has a very good digestion and absorption rate. It is easy to cultivate and harvest and shows strong alkalinity. Seaweeds are classified into blue, green, red, and brown according to the main color. Spirulina is a kind of blue green, and its color is emitted by phycocyanin (blue) of chlorophyll (green) in cells. These spirulina are known to have atopic prevention effect, skin aging suppression effect, and fat metabolism improvement effect, but studies on other physiological activities are still incomplete.

In one embodiment of the present application, the spirulina may be spirulina platensis or spirulina maxima. The lactic acid bacteria cultured in the culture medium containing them by using the spirulina platensis or spirulina maxima may have an effect of improving memory.

In one embodiment of the present application, the spirulina extract contains spirulina powder in water, anhydrous or hydrous lower alcohol of C1-4, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol and It may be extracted with a solvent selected from the group consisting of these combinations. According to one embodiment of the present application, the extraction solvent may be ethanol, wherein the concentration of the ethanol may be 50% to 90%, more preferably the concentration may be 70%. Meanwhile, the weight mixing ratio of the spirulina powder and the solvent may be about 1:1 to 100, and preferably about 1:10.

In one embodiment of the present application, the extraction method for obtaining the extract is any one of methods such as heating extraction method, hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, water vapor distillation method, ultrasonic extraction method, elution method or compression method It may be selected and used, but preferably, a heating extraction method or an extraction method using ultrasonic waves may be used.

In one embodiment of the present application, the added amount of the spirulina powder or spirulina extract may be 1 to 20 parts by weight, preferably 10 parts by weight, relative to 100 parts by weight of the total composition of the lactic acid bacteria culture medium. When the added amount of the spirulina powder or the spirulina extract is less than 1 part by weight relative to 100 parts by weight of the total composition of the lactic acid bacteria culture medium, the added amount of spirulina may be too small, and the memory improving effect of the lactic acid bacteria to be cultured may be lowered. The amount of the medium component may be too small to cultivate lactic acid bacteria well.

In one embodiment of the present application, the culture may be performed for 6 hours or more. When the culture is performed for less than 6 hours, the amount of lactic acid bacteria cultured may be small.

In one embodiment of the present application, the number of cells of lactic acid bacteria after culturing compared to the number of cells of lactic acid bacteria before inoculation may be increased by 60% to 150%, and preferably increased by 80% to 150%. Accordingly, the lactic acid bacteria cultured in the culture medium containing the spirulina powder or the spirulina extract may be cultured in a larger amount than the lactic acid bacteria cultured in the existing culture medium.

In one embodiment of the present application, the method for culturing the lactic acid bacteria for improving memory may be performed by a perfusion culture apparatus as shown in FIG. 1. In this case, the lactic acid bacteria culture medium may be prepared in the first vessel 1 of FIG. 1, and the spirulina powder or spirulina extract may be prepared in the second vessel 3. More specifically, after inoculating the lactic acid bacteria in the culture medium prepared in the first vessel (1), a portion of the culture medium inoculated with the lactic acid bacteria is transferred to the second vessel (3) and cultured, and the second vessel ( 3) by moving a portion of the culture medium moved to the first vessel (1) it may be repeated to perform the culture of lactic acid bacteria. At this time, the content of the culture medium to be moved between the vessels may be about 5 to 20% by volume compared to the total volume of the culture medium, preferably about 10% by volume.

The second aspect of the present application,

It provides a lactic acid bacteria for improving memory, cultured by the culture method according to the first aspect of the present application.

The third aspect of the present application,

It provides a health functional food comprising lactic acid bacteria for improving memory according to the second aspect of the present application.

The detailed description of parts overlapping with the first aspect of the present application is omitted, but the description of the first aspect of the present application may be equally applied even if the description is omitted in the second and third aspects.

Hereinafter, the lactic acid bacteria for improving memory according to the second aspect of the present application and the health functional food according to the third aspect of the present application will be described in detail.

In one embodiment of the present application, the lactic acid bacteria for improving memory may be formulated into a pharmaceutical composition including one or more pharmaceutically acceptable carriers for administration.

In one embodiment of the present application, the pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectables, etc. You can. For example, for formulation in the form of tablets or capsules, the active ingredient can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and colorants can also be included in the mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural sweeteners such as corn sweeteners, acacia, trachocanth or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants may include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like. Acceptable pharmaceutical carriers in compositions formulated as liquid solutions include, as sterile and biocompatible, saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary.

In one embodiment of the present application, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules, or tablets. Furthermore, it can be preferably formulated according to each disease or component using methods disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by appropriate methods in the art.

In one embodiment of the present application, the lactic acid bacteria for improving memory may be used as a health functional food, and in this case, an additional food additive may be added.

In one embodiment of the present application, the suitability as the food additive can be determined by the standards and standards for the item in accordance with the general rules and general test methods of food additives approved by the Food and Drug Administration, unless otherwise specified. have.

In one embodiment of the present application, items listed in the "food additive revolution" include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamonic acid; Natural additives such as decolorant, licorice extract, crystalline cellulose, high pigment, and guar gum; And mixed preparations such as L-sodium glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar colorants.

In one embodiment of the present application, when the lactic acid bacteria for improving memory is used as a dietary supplement, granulate a mixture of lactic acid bacteria with excipients, binders, disintegrants and other additives, and then granulate, It can be put into compression molding, or the mixture can be directly compression molded. In addition, the health functional food in the form of tablets may contain a mating agent or the like as necessary.

In one embodiment of the present application, the lactic acid bacteria for improving memory may be prepared in the form of a soft capsule for use as a pharmaceutical composition or a health functional food, wherein the soft capsule is in a shell for the soft capsule and the shell It may be to include the capsule filling material accommodated. At this time, the capsule filler may be lactic acid bacteria for improving the memory, and the soft capsule has excellent and characteristic properties such as lack of physical strength, disintegration ability, odor, taste, color, and adhesion, as well as excellent aging stability. Can be represented.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

Example. Lactobacillus plantarum in a culture medium containing spirulina HY-08)

In order to cultivate Lactobacillus plantarum which is a lactic acid bacteria, a perfusion culture apparatus as shown in FIG. 1 was prepared. The perfusion culture apparatus is a place where basic culture is performed, and is a place where microorganisms are cultured using an initial general medium. The culture medium of the perfusion culture apparatus is distilled water in yeast extract (yeast extract) 0.5 wt%, peptone (peptone) 1 wt%, glucose (glucose) 2 wt%, magnesium sulfate (magnesium sulfate) 0.01 wt%, manganese sulfate ( manganese sulfate) was prepared by adding 0.005 wt%, potassium phosphate 0.2 wt% and polysorbate 80 0.1 wt%. The culture medium was sterilized, and the sterilized culture medium was inoculated with Lactobacillus plantarum which is a lactic acid bacteria, and cultured at a temperature of 37°C.

Thereafter, a portion of the cultured medium for 24 hours was extracted with spirulina (the spirulina extract was prepared as follows: first, spirulina powder and 70% ethanol were mixed at a weight ratio of 1:10, and then the mixture was subjected to an ultrasonic extractor ( AUG-R3-900, ASIA ULTRASONIC, Gyeonggi-do, Korea) was pretreated at 120 kHz for 8 hours at room temperature, after which the sonicated mixture was heated at a temperature of 65° C. for 4 hours to extract After that, the extract was concentrated using a vacuum decompressor (Rotary Vacuum Evaporator NN series, EYELA, Rikakikai Co., Tokyo, Japan) and then used a freeze dryer (PVTFA 10AT, ILSIN, Suwon, Korea). The mixture was transferred to the reactor 3 of FIG. 1 containing 10 wt% of the culture medium by adding 10 wt% of the composition to the culture medium and sterilizing), and then cultured for 24 hours. . After that, it was cultured by repeating the transfer to reactor 1 again. The amount of the culture medium moving from each reactor to the reactor was moved at a rate of 10% of the total volume, and cultured for a total of 360 hours.

Comparative Example 1. Lactobacillus plantarum (Lactobacillus plantarum) in a culture medium containing no spirulina HY-08)

The lactic acid bacteria were cultured in the same manner as in the above example, except that the spirulina extract was not added to the culture medium in the above embodiment but cultured in a general culture tank.

Comparative Example 2. Preparation of scopolamine

As a comparative example, a commercially available scopolamine was purchased and prepared.

Comparative Example 3. Preparation of donepezil

As a comparative example, a commercially available donepezil was purchased and prepared.

Experimental Example 1. Measurement of cell growth of lactic acid bacteria

In order to measure cell growth over time of the lactic acid bacteria cultured according to the culture method of the present invention, the lactic acid bacteria (Lactobacillus plantarum) cultured in Example 1 and Comparative Example 1 were cultured for 360 hours and the cell growth was measured. , The results are shown in FIG. 2.

As a result, as shown in FIG. 2, it was confirmed that the cell growth of the lactic acid bacteria cultured in Example was higher than that of the lactic acid bacteria cultured in Comparative Example 1 as the culture time passed 6 hours. In addition, it was confirmed that the cell growth of lactic acid bacteria was amplified once again when the feed was performed once.

Therefore, it was confirmed that cell growth was significantly higher in the case of lactic acid bacteria cultured according to the embodiment of the present invention.

Experimental Example 2. Measurement of underwater maze activity

In order to measure the degree of memory improvement of the lactic acid bacteria for improving memory of the present invention, the rats that did not administer anything (control) and the rats administered 200 mg/kg or 400 mg/kg of lactic acid bacteria cultured in the examples, in Comparative Example 1 Rats administered with the cultured composition 200 mg/kg or 400 mg/kg, rats administered with scopolamine prepared in Comparative Example 2, and rats administered with donepezil prepared in Comparative Example 3 The maze activity in water was measured by the following method.

First, the rats used in this experiment were 4 week old SPF male ICR mice, reared for 1 week and subjected to an adaptation period. During the adaptation period, the weights of the rats were measured, and the selected rats were distributed as uniformly as possible to fit the average weight of each group so that the specified number was 22±2°C, relative humidity 50±5%, and ventilation was 10-15 times/ The environmental conditions of hr, lighting time 12 hours (7:30 a.m. lighting-7:30 p.m. lighting), and illuminance 150 to 300 Lux were set. The underwater labyrinth test of the rats was carried out using an animal cognitive ability tester (EthoVision Maze test system, Noldus Information Technology, Wageningen, Netherlands). When the rats remembered the markers around the tank and visited the refuge located at a certain place in the tank and stayed for more than 10 seconds, the time taken to visit the escape was set as escape latency. The average value was taken as the mean escape latency. The experiment was conducted twice a day for 4 days in a row, and if the rat did not find the escape within 120 seconds, the rat was placed in the escape for 10 seconds, and the experiment was completed and the escape latency was set to 120 seconds.

The results are shown in FIG. 3, and as shown in FIG. 3, in the case of the rats administered with the lactic acid bacteria for improving memory, which were cultured in the Examples, it was confirmed that the escape latency was significantly reduced as the date passed. Therefore, it was found that the lactic acid bacteria for improving memory of the present invention is effective in improving memory.

Experimental Example 3. Measurement of passive avoidance activity

In order to measure the degree of memory improvement of the lactic acid bacteria for improving memory of the present invention, the rats that did not administer anything (control) and the rats administered 200 mg/kg or 400 mg/kg of lactic acid bacteria cultured in the examples, in Comparative Example 1 Rats administered with cultured lactic acid bacteria 200 mg/kg or 400 mg/kg, rats administered with scopolamine prepared in Comparative Example 2 and rats administered with donepezil prepared in Comparative Example 3 The subject was measured by experimenting with passive avoidance activity in the following manner.

A manual evasion box (GEMINITM Avoidance System, San Diego Instrument, San Diego, CA, USA) was used to perform a manual evasion test. In a bright room of a passive evasion box divided into a dark room and a bright room, rats were placed in a dark room. When passed, an electric shock was applied for 2 seconds. When the rats were placed in the bright room again 24 hours after the electric shock, the nature of the dark room was suppressed and the nature of going to the dark room was suppressed. At this time, the memory was evaluated by measuring the step throughlatency.

The results are shown in FIG. 4, and as shown in FIG. 4, it was confirmed that in the case of the rats administered with the lactic acid bacteria for improving memory, which were cultured in the Example, the residence time in the bright room was high. Therefore, it was found that the lactic acid bacteria for improving memory of the present invention is effective in improving memory.

Claims (10)

Preparing a lactic acid bacteria culture medium;
Sterilizing the culture medium of the lactic acid bacteria;
Inoculating the sterile lactic acid bacteria culture medium; And
Culturing the lactic acid bacteria by adding spirulina powder or spirulina extract to the lactic acid bacteria culture medium inoculated with the lactic acid bacteria;
Method for culturing lactic acid bacteria for improving memory, including.
According to claim 1,
The culture medium of lactic acid bacteria, distilled water, yeast extract, peptone, glucose, magnesium sulfate, manganese sulfate, potassium phosphate, polysorbate 80 (polysorbate 80) and a method for culturing lactic acid bacteria for improving memory, comprising a material selected from the group consisting of combinations thereof.
According to claim 2,
The content of the yeast extract relative to 100 parts by weight of the total composition of the lactic acid bacteria culture medium is 0.1 parts by weight to 1 part by weight, the content of peptone is 0.1 parts by weight to 2 parts by weight, and the content of glucose is 1 part by weight to 5 parts by weight, The content of magnesium sulfate is 0.001 part by weight to 1 part by weight, the content of manganese sulfate is 0.001 part by weight to 1 part by weight, the content of potassium phosphate is 0.01 part by weight to 1 part by weight, the content of polysorbate 80 Is 0.001 part by weight to 1 part by weight of lactic acid bacteria culture method for improving memory.
According to claim 1,
The spirulina spirulina platensis (spirulina platensis) or spirulina maxima (spirulina maxima) culture method of lactic acid bacteria for improving memory.
According to claim 1,
The spirulina extract is derived from the group consisting of water, C1-4 anhydrous or hydrous lower alcohol, acetone, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, 1,3-butylene glycol and combinations thereof. Method for culturing lactic acid bacteria for improving memory, which is extracted with a selected solvent.
According to claim 1,
The amount of spirulina powder or spirulina extract added is 1 part by weight to 20 parts by weight compared to 100 parts by weight of the total composition of the lactic acid bacteria culture medium The method for culturing lactic acid bacteria for improving memory.
According to claim 1,
The lactic acid bacteria include Lactobacillus plantarum, L. casei, L. acidophilus, Lactobacillus L. bulgaricus, Bifidobacterium B. longum, B. bifidum, B. bifidum, Actiregularis, L. rhamnosus, and lactic acid bacteria selected from the group consisting of combinations thereof Method for culturing lactic acid bacteria for improving memory.
According to claim 1,
The culture method of lactic acid bacteria for improving memory is to be performed for more than 6 hours.
A lactic acid bacteria for improving memory, cultured by the method of claim 1.
A health functional food containing lactic acid bacteria for improving memory of claim 9.

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