KR20200034400A - Fungicidal composition for controlling plant diseases and production method thereof - Google Patents

Abstract

The present invention relates to a sterilizing agent for plant diseases produced by combining sulfur and a stevia fermentation strain obtained from a stevia plant. The sterilizing agent has counterveiling power against phytopathogenic fungi, especially anthrax, powdery mildew, gray mold, Rhizoctonia solani and filamentous fungi, and promotes the growth of plants by blocking the penetration of fungi or inhibiting proliferation even by spraying a predetermined amount of plants.

Description

Fungicidal COMPOSITION FOR CONTROLLING PLANT DISEASES AND PRODUCTION METHOD THEREOF}

The present invention relates to a fungicide for plant diseases that can promote the growth of plants by blocking pests or inhibiting proliferation occurring in plants.

Phytopathogenic fungi, which occur in horticultural crops, among various crops, directly or indirectly harm horticultural crops, so to harvest a lot of high-quality horticultural crops, spray chemical pesticides or partially promote growth or inhibit growth. Doing.

Phytopathogenic fungi are a major cause of deterioration in quantity and quality by causing damage to crops such as various vegetables, fruits, and flowers, and ginseng spotted deciduous bacteria (Alternaria alternata), gray mold fungus (Botrytis cinerea), and strawberry flower fungi cladosporioides), pepper anthracnose (Colletotrichum gloeosporioides), ginseng root fungus (Cylindrocarpon destructans), melon vine rot (Didymella bryoniai), tomato wilt (Fusarium oxysporum), tomato leaf fungus (Fulvia fulvum) Many botanical pathogens, such as fujikuroi), red pepper blight (Phytophthora capsici), rice leaf rot (Rhizoctonia solani), sweet potato rot (Rhizopus nigricans), onion Sclerotium cepivorum (Stemphylium lycopersici) There is.

Among them, late blight has a strong pathogenicity to host plants and is a primary invader that breaks down and invades the cell wall of the host even if there is no wound, most of which belong to Phytophthora. Since late blight does not invade diseased tissues secondarily, late blight isolated from diseased plants is the main cause of the disease.

In particular, pepper blight greatly reduces the production of pepper when cultivated, and pepper blight, which causes pepper blight, is a semi-aquatic bacterium that evolved from water and settled on water and propagates along the water. It is known that it is best to prevent the incidence of the disease, because once it occurs, it is best to prevent the occurrence of the disease, and it is important to prevent the proliferation of pathogens by preventing the occurrence of the disease by proactively applying a registered drug. In addition, it is important to prevent further spread if the disease has already occurred.

In order to control late blight, methods such as cultivation of resistant varieties and crop rotation are also performed, but the most effective control method so far is a chemical control method using an organically synthesized fungicide. The spraying amount of pesticides due to such chemical control is increasing every year, and due to the excessively used chemical pesticides, the resistance of plant pathogens to chemical pesticides is obtained, the residual toxicity of chemical pesticides, acidification of soil, toxicity to livestock, and effects on natural enemies , Damages such as pollution of drinking water sources and destruction of ecosystems are getting serious day by day, and social problems are also getting serious.

Therefore, Korea has recently spurred the production of eco-friendly agricultural products, and a method of controlling pests by applying a sterilizing agent of natural ingredients rather than organic synthetic pesticides to the leaves of plants has been spreading.

In particular, the frequency of using pest control and sterilizing agents containing sulfur as a main component is increasing, but when about 3 months have passed since the manufacturing of pest pesticides in which existing sulfur is mixed, the sulfur component of the fine powder is precipitated at the bottom of the container. , It was separated from water, and after the clogging and spraying of the spray nozzle due to the sediment, problems such as entanglement without being evenly applied to the leaf surface of the plant were exposed.

Republic of Korea Patent Application No. 10-2012-0080467

The present invention sprays pesticides of chemical components to remove and block pests such as phytopathogenic fungi occurring in various plants, and acquires resistance to chemical pesticides of plant pathogens due to overused chemical pesticides, residual toxicity of chemical pesticides , To solve problems such as acidification of soil, toxicity to livestock, effects on natural enemies, pollution of drinking water sources, and destruction of ecosystems. Disinfectants of natural ingredients using sulfur and stevia fermentation strains, not organic synthetic pesticides, are applied to plants. Therefore, the main purpose is to provide a fungicide for plant pests to control pests.

However, the problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

Therefore, the present invention for solving the above problems, water, sulfur, quicklime, biotite, muscovite, mica, sericite, paragonite, haerite, illite, margarite, and any one selected from the group of cleantonite It is characterized in that it is formed by mixing the sterilized fermentation strain to further form a sterilizer stock solution by mixing.

In addition, the method for producing a plant pesticide of the present invention, water, sulfur, quicklime, biotite, muscovite, gold mica, sericite, paragonite, halorite, illite, margarite, kryptonite Mixing step of generating a mixture of liquid by mixing one more (S100);

Adding water to the mixture, and heating the mixture to a predetermined temperature to sinter the mixture (S200);

A mixture separation step of storing the mixture in the precipitation tank for a predetermined time, separating the fine crystals mixed with the mixture into the lower part of the precipitation tank, and separating the liquid mixture of pure water from which the fine crystals are separated in the precipitation tank (S300);

Purifying the mixture separated from the sedimentation tank with a filter to obtain a sterilizing agent stock solution to generate a sterilizing agent stock solution (S400); And

And administering a stevia fermentation strain obtained from a stevia plant to the sterilizing agent stock solution, ripening for a predetermined time to complete the sterilizing agent, and commercializing the product (S500).

The present invention is a sterilizing agent for plant diseases prepared by combining sulfur and a stevia fermentation strain obtained from a stevia plant, and has anti-galling power to phytopathogenic fungi, particularly anthrax fungi, rare powdery mildew, gray mold fungus, mozarlococcus fungus, filamentous fungi, etc. It has an excellent effect and has a remarkable effect of preventing the growth of plants by blocking the penetration of fungi or inhibiting proliferation even by spraying a predetermined amount on the plants.

In describing the preferred embodiments of the present invention in detail, detailed descriptions of related well-known components or functions are omitted when it is determined that the subject matter of the present invention may be obscured.

The sterilizing agent for plant combination of the present invention is water, sulfur, quicklime, and any one selected from the group consisting of biotite, muscovite, muscovite, sericite, paragonite, haerite, illite, margarite, and kronatonite. To form, it is formed by further mixing the stevia fermentation strain in the stock of the fungicide.

In addition, the method for producing a plant pesticide of the present invention, water, sulfur, quicklime and biotite, muscovite, gold mica, sericite, paragonite, haerite, illite, margarite, any one selected from the group of cleantonite Mixing step to produce a mixture of a liquid mixture by mixing (S100); Adding water to the mixture, and heating the mixture to a predetermined temperature to sinter the mixture (S200); A mixture separation step of storing the mixture for a predetermined time in a sedimentation tank, separating fine crystals and foreign substances mixed in the mixture into a lower portion of the sedimentation tank, and separating the liquid mixture of pure water in which fine crystals and foreign substances are separated from the sedimentation tank (S300); Purifying the mixture separated from the sedimentation tank with a filter to obtain a sterilizing agent stock solution to generate a sterilizing agent stock solution (S400); And administering a stevia fermentation strain obtained from a stevia plant to the sterilizing agent stock solution, ripening for a predetermined time to complete the sterilizing agent, and commercializing the product (S500).

When the plant is cultivated, the present invention can suppress or prevent diseases caused by fungi such as anthracnose, rare powdery mildew, red mold, eczema fungus, gray mold, mozzaric disease, filamentous fungus, etc. It relates to a fungicide for plant diseases that can be grown safely and reliably from.

A method for manufacturing a plant pesticide and a plant pesticide according to the present invention for achieving the above object will be described in detail through examples.

The sterilizing agent for plant diseases of the present invention is first mixed with water, sulfur, quicklime and biotite in a certain ratio.

Preferably, for 15 liters of water, 15 kg of sulfur, 7 to 8 kg of quicklime, and 1 to 2 kg of biotite are prepared and mixed.

The sulfur is preferably prepared in the form of a powder without lumps, but is not limited to either powder or liquid.

The biotite (백, biotite), muscovite (白雲母, muscovite), mica (金 雲母, phlogopite), sericite (絹雲母, sericite), paragonite (paragonite), haeseokseok (海 綠石, glauconite) , It can be replaced with any one selected from the group of illite, margarite, and clintonite.

Add sulfur and water to a mixing tank of a given size, boil over an open fire and stir so that the sulfur is well dissolved in the water.

When the sulfur boils inside the mixing tank and air bubbles rise, the quicklime and biotite are gradually added to the mixing tank. At this time, the chemical reaction of sulfur and quicklime and / or biotite in the mixing tank may occur intensely, and as a result, the contents of the mixing tank may overflow, so the amount of quicklime and / or biotite is appropriately adjusted and mixed. Put into the jaw and stir it.

The present invention may further mix the quicklime and the biotite in a mixing tank in which water and sulfur are mixed, to produce a liquid mixture, or may mix the quicklime and the biotite, and input these combinations into a mixing tank containing sulfur and water.

Since the sulfur has a melting point of 112.8 ° C, it cannot be completely dissolved in the water that cuts the sulfur, so that the sulfur can be completely melted by generating high temperature heat by chemical reaction by adding the quicklime.

The biotite melts due to high heat to provide a large amount of mineral components, and can be replaced with any one selected from the group of muscovite, gold mica, sericite, paragonite, calcite, illite, margarite, and clintonite. .

When an intense chemical reaction occurs in the mixing tank, it is desirable to prevent the mixture inside the mixing tank from overflowing by adjusting the amount of quicklime or controlling the heating temperature.

Further, water, sulfur, quicklime and biotite are mixed in the mixing tank to form a liquid mixture, and the mixture is boiled until about 60L remains inside the mixing tank (mixture generation step (S100)).

Next, a chemical reaction occurs as water, sulfur, quicklime and biotite are mixed in the mixing tank, a mixture is formed, and when the chemical reaction is stopped, the mixture is moved to the firing tank. In addition, a calcination step of heating to a predetermined temperature by adding more water to the calcination tank containing the mixture is performed (mixture calcination step (S200)).

When the chemical reaction of the mixture is over, 20 to 30 L of water is further added to the firing tank, and heated for a predetermined time with a fire. At this time, boil until the mixture inside the firing tank remains about 60L. Then, in the state where the mixture remains in the sintering tank, about 20 to 30 L of water is additionally filled, and the mixture inside the sintering tank is heated again so that only about 70 L remains.

Then, the mixture obtained through the calcination step (S200) is transferred to a clean sedimentation tank and stored for a predetermined time (about 1 to 2 days), and fine crystals or foreign substances mixed in the mixture are sedimented to the bottom of the sedimentation tank. Fine crystals or foreign substances and the pure liquid mixture from which the precipitate is removed are separated into upper and lower layers, so that only the upper pure liquid mixture in the precipitation tank can be easily separated (mixture separation step (S300)).

Then, the liquid mixture separated from the sedimentation tank is transferred to a purification tank, and purified through a filter provided in the purification tank to obtain a sterilizing agent stock solution (a sterilizing agent stock solution obtaining step (S400)).

The filter used in the present invention includes a charcoal filter, a biotite filter, a company serpentine filter, a crystal filter, and a cell culture filter in which mack-rock and mud are mixed.

The pure mixture separated from the sedimentation tank and transferred to the purification tank, first passes through a charcoal filter, and then passes through a biotite filter, your serpentine filter, a crystal filter, and a cell culture filter in which mack-rock and mud are mixed in order to filter in 5 steps. It is purified by.

The order in which the mixture passes through the filter is not limited to the above order and can be changed.

The filter is used to form a ball having a smooth flow of water in a heating furnace of 1,200 ° C, and to use it by firing, and it is highly efficient to construct the ball with a small ball having a diameter of 7 mm.

The cell culture filter in which the mackban-stone and mud are mixed, the mackban-stone and mud are mixed in a 5: 5 ratio, and then the composition is prepared by the filter manufacturing method, and the prepared strain is coated. It is preferable to use the cultured strain in water for about a week.

On the other hand, the sterilant stock solution obtained through the filter in the refining tank is transferred to a ripening tank set at 40 to 45 ° C, where the stevia fermentation strain obtained using stevia plants is administered to the sterilizer stock solution, and aged by about 3 days. The plant fungicide according to the product is completed and commercialized. (Production step (S500)).

The Stevia plant is a perennial succulent plant of the Asteraceae family, and grows around rivers and wetlands. The root has no clear root development and there are many side and root roots. In the later stages of growth, thick roots develop and store function. New branches grow in the hidden eyes near the roots, forming new stems every year. Stems stand upright and the functions of the stems disappear during winter. Leaves are running on the stem node, and branches sprout from the axilla. Leaves are opposite, lanceolate, 4-10 cm long, and about 2.5 cm wide. It has fine teeth and curves, and has 3 leaf veins. There are no petioles, and fine hairs are formed throughout the aeration. Flowers bloom from 5 to 6 ornamental flowers in a single flower and have properties of self-incompatibility. Since it is a single plant, flowers bloom quickly under a single condition. Seeds have a low deletion rate and are very small. There is a plum-shaped tubular hair at the end of the seed. It is native to the border mountains of South America, such as Paraguay, Argentina, and Brazil. In Paraguay, Stevia leaves have been used as sweeteners for a long time. Recently, when the harmfulness of synthetic sweetener saccharin became a problem, it attracted attention again. The leaves contain a stevioside, a sweetener, about 6 to 7% by weight, the content of which varies greatly among individuals. The sweetening ingredient is 300 times that of sugar, making tea for drinking or as a substitute for gum, and as a sweetener for soft drinks. In Korea, it has been brought in in 1973 to develop new varieties. Breeding is done with seeds or knives. Harvesting can be done 1-2 times a year, and the season is early and mid-September. Stevia contains potassium, sodium, beta-carotene, vitamin A, vitamin B2, vitamin B6, and vitamin E. It contains inorganic salts and nutrients necessary for the human body, and has powerful antioxidant, sterilizing, and detoxifying effects. In particular, it is known that it can be used for restoring soil or plants contaminated with dioxin because it has dioxin resolution.

Stevia fermentation strain using a stevia plant having the above characteristics can be cultured in the following way.

First, stevia plants are harvested and semi-dried (D100).

The stevia plant is most preferably grown to a size of about 30-50 cm.

Put the semi-dried stevia plant in a black plastic bag or blackout bag, and mix any one or at least two of Penicillin camemberti, Penicillin roqueforti and Penicillin glowfoam One was inoculated into Stevia plants (D200).

The penicillin camemberti, penicillin roqueforti, and penicillin glowform are strains that produce polysaccharides, and polysaccharides such as stevioside and rebaudioside contained in stevia are strains. It helps to become an activated polysaccharide that feeds. In addition, the penicillin camemberti and penicillin glow foams are induced to have allergens, and are effective in blocking severe mold allergies.

Stevia plants that have undergone the “D200” step are stored at a temperature of 35 to 40 ° C. for 7 to 8 days to allow strains to propagate in stevia (D300).

Then, about 5 kg of stevia in which the strain was cultured is put in 10 L of water and brewed for about 3 to 4 days. Therefore, the components of stevia and the cells cultured in stevia are soaked in water, thereby obtaining stevia extract (D400) ).

And finally, to the obtained stevia extract, a strain of Archaea, Deinococcus radiodurans, or Sulfolobus solfataricus is combined (D500).

The Deinococcus radiodurans strain is a representative strain belonging to the genus Dinococcus, and the cell membrane extract of the Denococcus radiodurans strain protects against UV rays and recovers damage from UV rays, preventing lipids and oxidation. , Whitening effect In particular, it has an excellent effect on anti-allergy.

The sulfolobus solfataricus is a sulfur (S) -dependent hyperthermophilic microorganism with an optimal temperature of 89 ° C., which is one of the most well-researched archaic bacteria and easily obtains a large amount of biomass. Can, and grows at very high temperatures, providing a source of enzymes with unusual physicochemical properties.

The present invention is to obtain a stevia fermentation strain having excellent anti-allergic efficacy by mixing the stevia extract with stevia extract obtained from a stevia plant, and administering the stevia fermentation strain to the stock solution of the sterilizing agent about 1 to 2%, which By aging for about 3 days, the fungicide for plant diseases according to the present invention is completed.

Therefore, the present invention is a sterilizing agent for plant diseases produced by combining sulfur and a stevia fermentation strain obtained from a stevia plant, and has anti-bacterial properties against phytopathogenic fungi, particularly anthrax fungi, rare powdery mildew, gray mold fungus, mozarlococcus fungus, filamentous fungi, etc. It has an excellent effect, and even by spraying a predetermined amount of the plant, there is a remarkable effect of blocking the penetration of fungi or inhibiting proliferation to promote plant growth.

The configuration described in the embodiments and drawings described above are only one of the most preferred embodiments of the present invention and do not represent all of the technical spirit of the present invention, and various equivalents and modifications that can replace them It should be understood that there may be examples.

Claims (6)

Water, sulfur, quicklime, biotite, muscovite, gold mica, sericite, paragonite, halorite, illite, margarite, and any one selected from the group of kryptonite are mixed to form a fungicide stock solution, and the fungicide stock solution A sterilizing agent for plant diseases, which is formed by further mixing a stevia fermentation strain.
According to claim 1,
The stevia fermentation strain,
Semi-dried Stevia plants were inoculated with either Penicillin camemberti, Penicillin roqueforti, or Penicillin glowfoam to cultivate the strain, and the Stevia plants having the strain in water A plant fungicide characterized by forming a stevia extract by soaking it for a predetermined time, and then forming the stevia extract by mixing it with a high bacterium strain.
According to claim 2,
The archaea strain is a fungicide for plant diseases characterized in that Deinococcus radiodurans (Deinococcus radiodurans) or sulfolobus solfataricus (Sulfolobus solfataricus).
In the manufacturing method of the plant pesticide,
Mixing step of water, sulfur, quicklime, biotite, muscovite, gold mica, sericite, paragonite, halorite, illite, margarite, kryptonite, and mixtures to create a mixture (S100) );
Adding water to the mixture, and heating the mixture to a predetermined temperature to sinter the mixture (S200);
A mixture separation step (S300) in which the mixture is stored in a precipitation tank for a predetermined time to precipitate the fine crystals mixed with the mixture to the bottom of the precipitation tank, and the pure liquid mixture from which the fine crystals are separated is separated from the precipitation tank;
Purifying the mixture separated from the sedimentation tank with a filter to obtain a sterilant stock solution to produce a stock solution of sterilizer (S400); And
Stevia fermentation strain obtained from a stevia plant is administered to the sterilant stock solution, matured for a predetermined time to complete the sterilizer, and commercialized (S500).
According to claim 4,
The stevia fermentation strain,
Semi-drying the stevia plant (D100);
After stevia plants are placed in a black envelope, a step of inoculating any of polysaccharide producing Cameniberti (Penicillium camemberti), Penicillin roqueforti (Penicillium roqueforti), or Penicillin glow foam (Penicillium glaucum) (D200);
Culturing and propagating the strain by storing the stevia plant at a temperature of 35-40 ° C for 7-8 days (D300);
Putting the stevia plant in water and brewing it for 3 to 4 days to form a stevia extract (D400); And
Method of manufacturing a bactericidal agent for plant diseases, characterized in that formed by the step (D500) of mixing a high bacterium strain to the stevia extract.
According to claim 4,
In the step of obtaining the stock solution of the fungicide,
The mixture separated from the sedimentation tank is a charcoal filter, a biotite filter, a company serpentine filter, a crystal filter, and a method for producing a fungicide for plant diseases, characterized in that it passes through a cultivation filter of a mixture of crushed stone and mud.