KR20200019433A - Spore seedling method of an agarophyte red alga, Gracilaria vermiculophylla - Google Patents

Abstract

The present invention relates to a method for collecting spores of Gracilaria verrucosa which comprises: a first step of drying leaves of Gracilaria verrucosa at a low temperature and inducing release of spores; a second step of attaching the leaves to a collection string and releasing carpospores; and a third step of arranging the collection string, to which the carpospores are attached, in a water tank and culturing the same. According to the present invention, a releasing amount of carpospores is increased.

Description

Spore seeding method of red agar stalks, agar raw material {Spore seedling method of an agarophyte red alga, Gracilaria vermiculophylla}

The present invention relates to a spore seeding method and a seedling device of the red algae stalk as an agar raw material. Specifically, the present invention relates to a spore seeding method of stalks, which can improve the discharge amount of the spores by controlling the release induction conditions of the stalks.

As a kind of red algae, Gracilaria vermiculophylla is a member of the family of the Spruce family, and inhabits intertidal zones such as Korea, Japan, Sakhalin Island, and Taiwan. In addition, it is common to see flirt attached to shallow seashore pebbles or piles, in which river water flows into the sea. In the meantime, 15 kinds of snails are found in Korea, and they grow wild in Goheung, Jangheung, Wando, Jindo, etc., and the growth period takes about 6-8 months.

Industrially, stalks are widely used as agar raw materials, and approximately 60% of agar produced around the world is raw stalks. In addition, flirt is rich in taste and nutrition is expected to be widely used in various fields. For example, the high content of iron, potassium and zinc is likely to expand the scope of use of flirt as a food ingredient, as well as nutrients and medicines in the future.

In Korea, however, stalks can be collected only during short periods of the year (mid-May to early July), making them difficult to access except inhabitants near the native waters. It is possible. Therefore, there is an urgent need for improvement of aquaculture methods for smooth supply and demand of fry.

Conventional technology related to seed production and aquaculture method using spores is Korean Patent Publication No. 10-0540071. The technique discloses a method for producing seedlings from the spores of red algae tuna genus. Specifically, it is a method of producing seedlings by releasing a large amount of spores by drying the mature leaf of the genus Noir. In a similar vein, Korean Patent Publication No. 10-0764515 discloses a method for producing seedlings from the imitation of Gadashishima. In particular, the imitation of Gadashishima is refrigerated to induce the release of spores.

However, the above-described prior arts share a limitation that the time required for shade is considerable. In addition, even through the above-described method, the amount of spores released is insignificant and it is insufficient for mass production of seedlings. In particular, there is no specific methodology for improving seedling production.

Korean Patent Publication No. 10-0540071 Korean Patent Publication No. 10-0764515

The present invention has been made to solve the above problems, and an object of the present invention is to provide a spore seeding method with improved discharge amount of the sperm superspores.

In addition, an object of the present invention is to provide a specific spore seedling method that can easily induce mass production of seedlings of stalks.

The present specification is a means for solving the above-described technical problem, spore seeding method for the red algae cultivation method is a first step of inducing spore release by low-temperature drying the stigma leaf; Attaching the leaf to the seedlings to release the superspores; The third step of culturing the seedling line attached to the superspores in a culture tank.

In addition, in each stalk spore seeding method of the present invention, the step 0 of collecting the stalk leaf having mature cystic follicles; preferably further comprises a.

Further, in each of the pods spore seeding method of the present invention, the temperature in the first step is preferably between 2 to 10 ℃.

In addition, in each of the pods spore seeding method of the present invention, the first step is preferably made for 1 to 5 hours.

In addition, in each pod spore seeding method of the present invention, the second step and the third step is preferably made in sterile seawater.

In each of the stalk spore seeding method of the present invention, the salinity of the sterilized seawater is preferably 35 psu.

In addition, in each pansy spore seeding method of the present invention, the roughness in the culture conditions in the third step is preferably between 40 to 60μmol photons m ^-2 s ^-1 .

In addition, in each pansy spore seedling method of the present invention, the culture conditions in the third step is preferably a temperature range of 22 to 24 ℃.

On the other hand, the present specification is a low temperature drying unit; Transfer unit for transferring from the low temperature drying unit to the water tank; A tank for culturing the superspores; Sterile seawater in the tank; And it further discloses a bale seeding device comprising a; air pump for supplying air to the tank.

Moreover, in each pansy seedling apparatus of this invention, it is preferable to further contain a culture liquid in sterile seawater.

The creeper spore seeding method of the present invention provides a spore seeding method of which the amount of release of superspores is improved by controlling the flesh release induction conditions.

In addition, the present invention provides a spore seeding method capable of mass production of seedlings by adjusting the spore release induction conditions and growth conditions.

1 is a photograph of a stigma slice with mature cysts.
Figure 2 is a graph showing the change in spore discharge amount according to the spore release method.
Figure 3 is a graph showing the change in spore discharge amount according to the change in temperature and the work amount of the low temperature drying.

The terms or words used in this specification and claims should not be construed as being limited to the common or dictionary meanings, and the inventors can appropriately define the concept of terms in order to explain their invention in the best way. Based on the principle, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention.

Therefore, since the configuration of the embodiments described herein is only one preferred example of the present invention, and does not represent all of the technical idea of the present invention, there may be various modifications that can replace them at the time of the present application. Should be understood.

Hereinafter, preferred embodiments of the present invention will be described in detail so that those skilled in the art may easily implement the present invention.

<1. Sprout seedling method of the present invention>

Herein, the first step of inducing spore release by low-temperature drying the stigma leaf; Attaching the leaf to the seedlings to release the superspores; Disclosed is a stalk spore seedling method comprising the third step of culturing the seedling line with the spores attached to the culture tank. In addition, in each stalk spore seeding method of the present invention, the step 0 of collecting the stalk leaf having mature cystic follicles; preferably further comprises a. Hereinafter will be described in detail by dividing each step.

In each of the pansy spore seedling method of the present invention, a 0 step of collecting the pansy leaves having mature follicles; preferably further includes. Pancreas cystaceae is a type of reproductive organ formed in the red algae spleen (配偶 體) refers to the pocket containing the spores. 1 is a photograph of a stigma slice with mature cysts. Referring to Figure 1, the maturation state of the stigmama can be visually confirmed. In the present invention, the follicles with 10 sacs matured and about 10 mm were harvested and used for spore seedlings.

Spore release from seaweeds is influenced by various environmental factors such as temperature, osmotic shock, moonshine and tidal rhythm, and roughness, so the method of inducing spore release differs depending on the species. Spontaneous discharge (SD), Desiccation shock, Low temperature shock (LT), Osmotic shock, Low temperature and desiccation shock (LT-D) Induce spore release in such a way.

2 is a graph showing a comparison of the amount of spore release according to the spore release induction method described above. Referring to Figure 2, the osmotic shock (Osmotic) was the lowest amount of spores, it can be seen that the amount of spores released from low-temperature drying shock (LD-T) is significantly higher. Therefore, when it is inferred based on the results shown in the graph, in order to secure a large amount of sperm spores, it can be said that the method of inducing spore release under low temperature dry shock.

That is, the present invention is characterized in that it provides a spore release induction condition that can exhibit a maximum spore release amount when inducing spore release through low temperature dry shock. In addition, in the case of natural release (SD), dry shock (Desiccations), low temperature shock (LT), it can be seen that the spore discharge amount is less than half of the low temperature dry shock. In fact, referring to FIG. 2, it can be seen that the synergistic effect of the low temperature shock and the dry shock at the same time compared to when the low temperature shock to the dry shock is independently performed.

On the other hand, when the low-temperature drying impact of the present invention is applied, in each flirt spore seeding method of the present invention, the temperature in the first step is preferably between 2 to 10 ℃. In addition, it is most preferable that the temperature is about 5 ° C. in the first step in that the highest spore emission can be ensured. If the temperature is less than 2 ℃ in the first step, there is a fear that the follicles are not mature enough or cold due to the excessively low temperature, as a result can be a problem that spores are not released. On the contrary, when the temperature exceeds 10 ° C., the low temperature shock is not applied to the fossa, and thus there is a problem in that the synergy effect due to the low temperature drying cannot be secured.

On the other hand, the first step is preferably made for 1 hour to 5 hours. In the first step, when the working condition is less than 1 hour, there is a problem that sufficient emission induction is not achieved due to the hasty condition change. On the contrary, when the working condition exceeds 5 hours, a sharp decrease in the amount of spores released due to damage to the cyst is a problem. Therefore, in order to induce a significant spore release amount of stigma spores, it is considered that the above working conditions are preferable.

In addition, in each pod spore seeding method of the present invention, the second step and the third step is preferably made in sterile seawater. In addition, the salinity of the sterilized seawater in the second step is more preferably about 35 PSU. The sterile seawater refers to sterile seawater in order to minimize environmental factors affecting the growth of the stigmata.

On the other hand, in the present invention, the release of the superspores of the second step is to put the leaves after the low temperature dry spore release induction on the seedling frame to release the spores to be attached to the seedling line. The seedling string can be used as a seedling string regardless of the thickness or type of string if the spores of the flirt can be attached well. In addition, the spores of the stalks are not damaged when released, it is preferable to be attached at a predetermined distance on the seedling frame so that it can be attached to the seedling line to grow.

In addition, in each stalk spore seedling method of the present invention, it is preferable that the third step is cultivated in a culture tank containing sterilized seawater, the seedling line attached to the spores discharged from the stalk leaves.

In the third step, the roughness in the culture conditions is preferably between 40 to 60 μmol photons m ^-2 s ^-1 . By adjusting the illuminance in the above range, it is possible to promote the growth of the superspores of the stalks. The light source for adjusting the illumination in the culture conditions of the third step was used as a fluorescent lamp. In addition, the adjustment of the illumination was controlled by adjusting the distance between the light source and the container containing the stigma and the spore itself, and confirmed by using a digital illuminometer.

<2. Scraping seedling device of the present invention>

The present specification is a low temperature drying unit; Transfer unit for transferring from the low temperature drying unit to the water tank; A tank for culturing the superspores; Sterile seawater in the tank; And it further discloses a bale seeding device comprising a; air pump for supplying air to the tank.

In the creeper seedling device of the present invention, after inducing the release of the superspores from the stalk leaf attached to the seedling line in the low temperature drying unit, the low temperature drying unit is transferred to the water tank.

The transfer unit for transferring to the water tank containing sterilized seawater from the low temperature drying unit may be provided with a rope roller capable of rotational movement, further comprising a control unit for adjusting the rotational speed of the transfer unit so that spores attached to the seedling line do not fall off. It may include.

In the flirt seedling device of the present invention, the <1. The description of "Gold spore seeding method> of this invention applies mutatis mutandis. Further, in each of the palsy seedling device of the present invention, it is preferable to further include a culture solution in the sterile seawater. The culture medium generally refers to a polyethersulfone (PES) culture medium used for cultivation of algae, and the culture medium may be included in sterile seawater at 25% for rapid growth of the snails.

Hereinafter, with reference to the accompanying drawings and embodiments will be described in more detail with respect to what is claimed by the present specification. However, the drawings and the examples presented in the present specification may be modified in various ways by those skilled in the art, and may have various forms, and the description of the present specification is not limited to the specific disclosed embodiments. It should be seen that all equivalents and substitutes included in the spirit and scope of the present invention are included. In addition, the accompanying drawings are presented to help those skilled in the art to more accurately understand the present invention may be shown exaggerated or reduced than the actual.

{Example and Evaluation}

Example. Low temperature drying temperature and work condition change

Example 1

Ten vesicles with cysts were collected and three of them were prepared. The leaves were put in three culture containers coated with paper towels, wrapped in foil, and blocked with light, and then low temperature dry shock was induced for 1 hour, 2 hours, and 3 hours in an incubator set at 2 ° C., respectively.

Example 2

Ten vesicles with cysts were collected and three of them were prepared.

Each of the leaves were placed in a culture medium coated with paper towels, wrapped in foil, and blocked with light, and then low temperature dry shock was induced for 1 hour, 2 hours, and 3 hours, respectively, in an incubator set at 5 ° C.

Example 3

Ten vesicles with cysts were collected and three of them were prepared. Each of the leaves were placed in a culture container coated with paper towels, wrapped in foil, and shielded from light, and then low temperature dry shock was induced for 1 hour, 2 hours, and 3 hours in an incubator set at 10 ° C., respectively.

evaluation. Spore Release Rate at Low Temperature Drying Temperature and Working Conditions

In accordance with Examples 1 to 3 of the present invention, the temperature and work conditions were changed, and a total of nine leaves subjected to spore release induction were placed in a culture vessel containing 15 ml of sterile seawater, respectively, and the number of spores released under an optical microscope was counted. .

1. Effect of low temperature drying temperature

Figure 3 shows the spore discharge amount according to the low temperature drying temperature and the work condition changes. Referring to FIG. 3, the number of spores released in Example 2 (temperature, 5 ° C.) was the highest, and in Example 1 (temperature, 2 ° C.) and Example 3 (temperature 10 ° C.), the number of spores released was almost the same. Similar results were found.

2. Influence of low temperature drying work

At each temperature condition according to an embodiment of the present invention, the amount of spores released according to the change in the amount of work was observed. Referring to FIG. 3 and Table 1, as a result of observing the release amount of spores by adjusting the amount of work in Example 2 (temperature, 5 ℃), it was confirmed that the number of spores released when the work amount is 2h. Moreover, also in Example 1 (temperature, 2 degreeC) and Example 3 (temperature, 10 degreeC), when the work amount was 2h, it was confirmed that the number of spores discharged was large.

Amount Example 1 Example 2 Example 3 2 ℃ 5 ℃ 10 ℃ 1h 634.33 ± 151.11 843.50 ± 147.90 562.93 ± 119.07 2h 707.23 ± 166.42 948.03 ± 169.75 666.53 ± 144.52 3h 598.13 ± 171.02 671.40 ± 204.81 410.82 ± 85.35

Claims (9)

A first step of inducing spore release by low temperature drying of the pansy leaves;
Attaching the leaf to the seedlings to release the superspores; And
Red algae herb spore seedling method comprising the; the third step of culturing the seedling line with the spores attached to the culture tank.
The method of claim 1,
Before the first step,
Red algae herb spore seedling method further comprises a zero step of harvesting the herb leaf with mature cysts.
The method of claim 2,
The first step is a red algae creeper spore seedling method, characterized in that the condition is made to block the light.
The method of claim 2,
In the first step, the temperature is between 2 to 10 ℃ red algae herb spore seedling method.
The method of claim 2,
The first step is a red algae creeper spore seedling method, characterized in that made for 1 to 5 hours.
The method of claim 1,
The second step and the third step is red algae creeper spore seedling method, characterized in that made in sterile seawater.
The method of claim 6,
The salinity of the sterile seawater is a red algae herb spore seedling method, characterized in that 35psu.
The method of claim 1,
In the third step, the roughness in the culture conditions is between 40 to 60 μmol photons m ^-2 s ^-1 red algae creeper spore seeding method.
The method of claim 1,
Cultivating spore seedling method of red algae stalks, characterized in that the culture conditions in the third step is a temperature range of 22 to 24 ℃.

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