KR100764515B1 - Artificial seedling production and farming method of Kadashima - Google Patents

Abstract

The present invention relates to an artificial seedling production and culture method of Gaedamashima, and more particularly, by cutting the intestines from Gaidashima imitation, which has been determined to be mature stage by tissue cutting, and cultured at the temperature and roughness suitable for growth It will increase the apoptotic activity and consequently enable the artificial production of seedlings. In addition, artificially produced seedlings can be used for the large-scale farming of Kadashima Island, which will gradually decrease in habitat to restore endangered Kadashima colony, diversify seaweed farming varieties, and spread farming technology to contribute to the increase of income of fishers. Can be. In addition, it can be used as a source of medicine and health food raw materials that create high value through mass production of Kadashima.

Kadashima, artificial seedlings, aquaculture

Description

Method of artificial seed production and industrial culture method for Kjellaniella crassifolia}

1 is a view showing a picture of a Kadamashima with a clear dragon pattern.

Figure 2 is a diagram showing a photograph of a mature ganoderma simula with a vesicle plaque.

Fig. 3 is a diagram showing a photograph in which the sleeping vesicles are collected from a Kadashima imitation and releasing dwellers from them.

Fig. 4 shows the micrographs of mature gonads and cell divisions in the Kadashima imitation.

5 is a diagram illustrating a process of incubating the resident in the plate.

Fig. 6 is a diagram showing a microscopic picture of apoptosis.

7 is a diagram showing the growth of Kadashima strainer according to temperature.

8 is a view showing the growth of the Kadashima sake in accordance with the illuminance.

9 is a view showing a photograph of the seedling produced by the glass culture.

Fig. 10 is a diagram showing a photograph of a dog shimama (seeds attached to a seedling line, engaged) produced by artificial seedlings.

11 is a view showing a photograph of a seedling seedling transplanted for excellent imitation production.

FIG. 12 is a diagram illustrating an indoor adaptation test and a growth test procedure at a depth of 30 m of a Kadashima seedling.

FIG. 13 is a view showing growth of Gadashishima according to water depth. FIG.

14 is a diagram illustrating a culture process of Gadamashima.

15 is a diagram showing a Kadashima fish farm facility drawing.

The present invention is Gadshima ( Kjellaniella) crassifolia ) relates to artificial seedling production and aquaculture methods.

There are 300,000 species of creatures in the sea, and 80% of the animals and plants of the world live in the sea, and the seaweeds in the sea are the primary producers, and the habitats of various animals such as invertebrates, fish and protozoa It is in a position to play an important role in marine ecosystems. In addition, it serves as a food for abalone, sea urchin, and shellfish, and has been used as a source of vitamins, minerals and various physiologically active substances as well as food, and as a fertilizer for livestock feed or crops.

A total of 735 seaweeds inhabit in Korea, and about 20 species of edible or industrially used foods are limited to a few species such as laver, seaweed, seaweed, seaweed, and seaweed. Therefore, in order to efficiently use the seaweed resources and diversify the variety of the seaweed farming industry, it is necessary to make efforts to develop various farming target species.

In the coast of Korea, the East Sea has severe changes in the marine environment, such as the crossing of the Great Manna Current from the north and the descending Korean Wave from the North. The algae inhabiting the east coast have a current form as a result of their adaptation to the habitat environment of a habitat for a long time. In particular, algae are inferior biota and contain many genetically unstable traits. For example, when algae adapted to a certain environment are transplanted into different habitats, their expression traits may appear differently depending on the new habitat. In addition, maintenance is very difficult because of the meiosis that occurs on the alternation of algae, even with new traits. For this reason as well as for aquaculture with living organisms, it is very difficult to predict how the transplantation of any species will affect the existing marine ecosystem. There are many examples of terrestrial animal and plant transplants, and their destruction of ecosystems is serious. Methods for the development and conservation of genetic resources will be developed and should be urgently addressed for future biological resource management already being implemented in developed countries. Separation and preservation techniques of female and female organisms, tissue culture, protoplast separation and development of regeneration techniques are essential for seed bank operation in terms of the availability of seed production throughout the year and the availability of seeds.

The kelp plants distributed in Korea can be divided into Laminaria in the north of Wonsan Bay and Kjellmaniella in the south. Gadamashima, also called indigenous Tashima, is a seaweed seaweed that lives mainly in deep waters below the low ships off the coast of Gangwon-do.

Kjellaniella crassifolia ( Kjellaniella crassifolia ) is a perennial brown algae belonging to the kelp family Kelpaceae. The Japanese name is Kagome. The root shape of Gadamashima is fibrous, rotatively rooted, with a leaf length of 1 to 2 m, and a leaf width of 20 to 30 ㎝, relatively broad. The thickness is 2 to 3 mm, the middle part is 1/3 to 1/4 of the leaf width, and there are dragon patterns on both sides of the middle part, and it becomes unclear when aging. The lower part of the stem is circumferential, the upper part is circular, and is 4-6 cm in length and 10-12 mm in diameter. It lives in the lunch zone, and after the fluid appears after December, it grows sufficiently until the next autumn, and when the user releases it, it does not grow more because of the end recording of the leaf, but it grows again in November. Beginning with the first year, the leaf is pushed up to become a biennial leaf. In the second year, growth grows rapidly, and the distinction between one-year and two-year leaves is clearly visible. The papules occur only on one side but gradually on the other side.

Gadshima is inhabited from 20 to 30 m deep in the northern coast of Gangwon (Goseong-gun, Ayajin, Sokcho-si, Gangneung-si). About 500 individuals per 10 m 2 were inhabited, but now 1 to 2 individuals per 10 m 2 have been inhabited, and the habitat area has been significantly reduced to 2 ha. It was distributed over about 1 ha in the sea area of Bongpo, and the density of habitat was found to be 1 individual per 10 m2. In addition, the area where it was inhabited in the past has not been found in the Gojin-gun Daejin and the early seas, and the Ayajin and Munam seas have been found but few disappeared.

In addition, the occidental plaque formation area of Aegidashima inhabiting the east coast was formed over 23% of the weight of the leaf body, but only about 5% in the case of Gaedamashima, which showed a very low reproductive capacity. Given these characteristics, once a habitat is destroyed, nature takes a long time to recover itself and there are many variables. Therefore, it is necessary to secure high-quality imitation for habitat restoration, but it is extremely rare in nature.

Gadamashima is more than twice as large as ordinary kelp, and contains more abundant fucoidan, glutamic acid, aspartic acid, minerals, etc., and has been used for food since ancient times. It is being developed as medicine, new substance and health food. However, in 1990, natural production amounted to 1,000 tons of biomass per year, but recently, indiscriminate sampling and typhoon threats have caused species to disappear, and the necessity of restoration and the production of high quality imitation are urgent. · Development of technology for separation and preservation of spouses is essential.

Therefore, the present inventors, while researching a method for restoring the habitat and mass production of seedlings in Gadashishima, found the conditions suitable for the production of Gadashima's materate without changing the genetic traits of Gadshima. Using the resulting seedlings in aquaculture, it was confirmed that mass production is possible to complete the present invention.

The present invention is to provide a method for producing artificial seedlings of Gaedamashima.

It is also an object of the present invention to provide a method for farming Gaedamashima.

The present invention provides a method for producing artificial seedlings of Gaedamashima.

In addition, the present invention provides a method for farming a sea bream.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention

(a) a harvesting step of obtaining a resident by collecting a vesicle plaque from a Kadashima imitation before the resident release; And

(b) providing a method of artificial seedling production of dog shimashima comprising the step of culturing the jujube to obtain a seedling.

More preferably, the step (b) to obtain a seedling by culturing the strainer

(1) culturing the strainer to obtain a spore;

(2) culturing the spores to obtain apoptosis; And

(3) culturing the apoptosis to obtain seedlings.

First, the drunkard is prepared from the Kadashima imitation before releasing the drunkard in the stage of the seeding in step (a). To produce seedlings of good-growing ganoderma lucidum, mature ganoderma cysts with porcine plaques are harvested. A cutaneous plaque forming region is cut out from the dummy and subjected to the drift stimulus by refrigeration in a dry and dry method at 3.5 to 4.5 ° C., preferably 4 ° C. for 11 to 13 hours, preferably 12 hours. Next, put the collected porcine bag part into a tank containing filtered seawater filtered through sterile seawater or a filter and stir slowly. After 8 to 12 minutes, preferably 10 minutes, the movement and density of the resident are examined under a microscope, and if the movement is visible at about 30 or more in a 100-fold microscope, it is determined to be a healthy and appropriate resident. The imitation is removed from the tank, and horizontally lined with a line or a line, so that the rider can attach and grow.

Next, in step (b), seedlings are obtained by culturing the drunk seeded in the step (a). The runners drawn in the state of being attached to a seedling line or a seedling frame are placed in a tank as soon as possible so as not to be dried, and cultured with a weak air supply. At this time, the culture is 35 to 45 days, preferably at a roughness of 1500 to 2500 lux, preferably 2000 lux at a temperature of 7 ~ 13 ℃, preferably 10 ℃, which is similar to the environment in which the Kadashima inhabit Incubate for 40 days. The resident forms a thread-shaped spouse consisting of tens of cells (step (1)), fertilizes eggs by making eggs and sperm, and then forms microscopic aposomes ( Step (2)) It becomes Gaidashima of the leaves of the new spores (step (3)). Seedling means a leaflet before the formation of the seed. The seedlings of about 1 cm that can identify the leaflets with the naked eye are preferable. It is unfavorable that the resident of Gadashishima is low or dies because of the growth of Gadamashima spores outside the range of the culture temperature and illuminance. This is best suited to the development of the drift and spores, ie, the kelp body, because Gadashishima is adapted to the cold environment of the deep sea. This step may further comprise a transplanting step of culturing suspended 2 ~ 4 m under water as it is immersed in the chaff frame, which is usually carried out to the size of the apoptosis is not buried in the diatoms or bunnies.

The artificially cultivated seedlings as described above can be used in aquaculture for mass production of Gadashishima.

In addition, the present invention

(A) transplanting the seedlings into the water drop and planting them at a depth of 4 to 6 m to form a first stage;

(B) Provides a method of farming Kadashima including the second step of farming the seedlings grown in step (A) at a depth of 20 ~ 30 m.

First, in step (A), the seedlings are transplanted to the lower plume and planted at a depth of 4 to 6 m, preferably 5 m, and the first culture. Depths of 7 to 13 ° C, preferably 10 ° C and 1500 to 2500 lux, preferably 2000 lux, which can be inhabited by transplanting the seedlings of the Kadashima to the water drop 3.5-6.5 at m, most preferably at 5 m. For aquatic invertebrates such as oysters, oysters, mussels, and oysters, such as kelp, substrates to which these organisms can attach, such as rafts and ropes, are required. Attachment substrates vary depending on the type of organism, and the attached attachment substrates are called Ha-yeon.

Next, in step (B), the seedlings grown in step (A) are grown at a depth of 20 to 30 m. Seawater temperature plays an important role in the culture of Kadashima Island. Since July, when the water temperature rises, the water temperature of 4 ~ 6m, preferably 5m, rises to the point that it is not suitable for Kadashima Island culture. For prevention and maturation, the water temperature should be from 20 to 30 m suitable for aquaculture.

The aquaculture may be carried out in a conventionally designed aquaculture farm, and when the above conditions are satisfied, it is expected that the kadashima will grow perennial.

The present invention provides the artificial seedling production and culture technology of Gadamashima based on the biological basic research such as the ecology and physiology of Gadamashima, and the separation and preservation of cancer and aquatic organisms, Diversification of seaweed farming varieties and the spread of farming methods can contribute to the income increase of fishers. In addition, it can be used as a source of medicine and health food raw materials that create high value through mass production of Kadashima.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Example  1: by environmental control according to the present invention Kadashima  Seedling production

1-1: zoospore  Emission stimulation experiment

Prior to preparing the resident from the Kadashima mock, the stimulation method was developed to develop an effective method to obtain the resident from the mock and effectively secure the resident.

The cutaneous plaques were cut out of the sampled Kadashima imitation and treated with four methods: 12 hours indoor dry, 30 minutes sun dry, immediate release or 12 hours refrigerated dry. Next, put the collected vesicles in sterile seawater treated with a transparent water tank installed in a flat place directly exposed to sunlight or filtered seawater filtered with a 1 ㎛ filter, and then stir slowly with a rod. From about 10 minutes, the movement and density of the resident were examined under a microscope, and when the subject showed more than about 30 subjects at 100 times of the microscope, the movement was determined to be a healthy and appropriate resident. The measurement was repeated 10 times.

The results are shown in Table 1.

division 12 hours indoor shade 30 minutes sunlight drying Release immediately after collection 12 hours refrigerated Residency (100 times view, dog) 35 ± 12 15 ± 9 20 ± 12 55 ± 18 Joist movements (visual observation) Somewhat active Somewhat active Somewhat active Very active

As shown in Table 1, the amount of release of resident in the 12-hour cold-drying imitation according to the method of the present invention, rather than in the Kadashima imitation, which stimulated the release of the resident by the method of 12-hour indoor shade, 30-minute sunlight drying or immediate release. The movement of the resident was also very active. Therefore, in the artificial production of the following Kadashima seedlings, a 12-hour refrigeration and drying method was used as the drift stimulating method.

1-2: Kadashima  From imitation Resident  Ready

For the production of seedling seedlings from the Kadashima imitation before releasing the owner The owner was prepared as follows.

In the imitation, the dragon pattern, which is characteristic of Gadamashima, was clear, the growth state in which the heart shape was apparent at the root part was good, and a mature enough one was used. Cut off the uterine plaque forming area from the imitation, give a stimulus to release by cold drying (approximately 4 ℃) for 12 hours, put it in a transparent water bath, and install it in a flat place where it is directly exposed to sunlight. The collected porcine pelvis was added to the filtrated seawater, which was then slowly stirred with a rod. The imitation was removed and the seedlings were placed horizontally and then chopped for about 2 hours.

Figure 1 shows a picture of a dragon pattern Gadamashima imitation, Figure 2 shows a picture of a mature ganoderma imitation formed with a vesicle plaque, Figure 3 shows the process of collecting the vesicle plaque and induces the release of the resident It was. In addition, microscopic photographs of the mature gonads and the spleens released therefrom by tissue cleavage are shown in FIG. 4.

1-3: zoospore  Incubation temperature suitable for growth

In order to determine the temperature suitable for the growth of the infusers of the dogs, the incubators of the dogs are grown as follows.

The strainer of Gadashishima prepared in Example 1-2 was made into a strainer containing about 50 strains per field of view under a microscope of 100x magnification. 2 ml of the fermented sap was inoculated gently sprayed into 12 cm Patridish. PESI (Table 2) was used as a medium, and growth and characteristics were analyzed under a microscope from day 1 of culture. Each of the three experimental groups were incubated at a temperature of 5, 10, 15, 20, 25, 30 ° C., and the medium was changed at weekly intervals. In addition, the illumination intensity was incubated for 40 days at 3,000 Lux and photoperiod as 12 hours light condition: 12 hours dark condition (12L: 12D). Observation was carried out under a microscope (100 magnification) during the cultivation to observe the formation of the spheroid, the growth state, etc., and the growth length of the apoliposome was measured after one month of culture.

Furtherance content Stock solution Na ₂ Glycerophosphate 1.0 g Sterilized in 970 ml of distilled water NaNO ₃ 7.0 g Tris HCl 8.14 g Tris Base or Tris 1.86 g or 10 g (pH 7.8) B vitamin mixture Vitamin B ₁₂ 1.0 mg / 100 ml 20 ml, filter sterilization Thiamine 50 mg / 100 ml Biotin 0.5 mg / 100 ml Fe (1: 1 molal solution with EDTA) Fe (NH ₄ ) ₂ (SO ₄ ) · 6H ₂ O 1.40 g / 2000 ml 500 ml, sterile Fe ₂ (NH ₄ ) ₂ (SO ₄ ) ₂₄ H ₂ O 2.12 g / 2000 ml Na ₂ EDTA 1.32 g / 2000 ml PⅡ trace metal mixture Boric acid (H ₃ BO ₃ ) 2.24 g / 2000 ml 500 ml, sterile 2000 ml Fe (FeCl ₃ ) .6H ₂ O 0.096 g / 2000 ml Mn (MnSO ₄ ) 0.24 g / 2000 ml Zn (ZnSO ₄ ) 7H ₂ O 0.044 g / 2000 ml Co (CoSO ₄ ) 7H ₂ O 0.0096 g / 2000 ml Na ₂ EDTA 2.00 g / 2000 ml KI (0.25 mg / ml) 10 ml Germanium dioxide mother liquor GeO ₂ 0.25 g / 1000 ml 4 ml / 2ℓ PES

The process of inoculating spores on the plate is shown in FIG. 5, the microscopic photograph of the apoptosis is shown in FIG. 6, and the degree of growth according to temperature is shown in FIG. 7.

As shown in FIG. 6, when observed under a microscope, the strainer adhered to the cover glass within 30 minutes in a few minutes to become a spore, but some movements were observed for about 3 hours. Germination of spores began to form after 1 day of culturing, and germination of spores occurred at 10 to 15 ° C in 3 days of culture. Spore germination was observed in all experimental conditions within 5 days at 5 ℃. In addition, the initial formation of the spheroid began to appear from the 6th day of culture and cell division began to occur. The female and female embryos began to be divided into 12 days of culture, and the ratio of female to male females was about 6: 4. The growth and maturation of female and female embryos first formed into apoptoses at 10 to 15 ℃ with high temperature conditions, and growth and maturation occurred first at low and low light conditions, even at high temperature conditions. The spores matured at 20 ° C. and progressed to the apoptotic phase. However, at 5 ° C., the male and female adolescents were very mature and progressed slowly to the apoptotic phase. The apoptotic culture was found to be the highest under the condition of 10 ~ 15 ℃.

In addition, as shown in FIG. 7, the experimental group of 5 ° C. showed slow development of spores, and the formation of spheroids occurred after about 25 days, and the growth after one month was 215 μm. After 10 months, the experimental group grew to 574 μm in the apoptotic state after one month, which was the best compared to the other experimental groups. After 15 months, the experimental group was grown to 480 ㎛ after one month, similar to the 10 ℃ experimental group, but the color was somewhat less vital. The 20 ° C. experimental group was grown to 320 μm one month later, and the 25 ° C. experimental group died at the spore stage in 17 days of culture, and the development rate was significantly slower than that of the other experimental groups. In addition, the 30 ℃ experimental group died in 7 days of culture, and did not form a spore, stayed in the cell migration stage.

Therefore, it was confirmed that the most suitable temperature for spore growth of Gadashishima was 10 ° C.

1-4: zoospore  Roughness measurement suitable for growth

In order to elucidate the illuminance suitable for the growth of the spores of Gaedamasma for the production of the cabbage seedlings of the present invention, the runners of Gaidashimas were cultured as follows.

The strainer of Gadashishima prepared in Example 1-2 was made into a strainer containing about 50 strains per 100x magnification field of view. 2 ml of the fermented sap was inoculated gently sprayed into 12 cm Patridish. PESI (Table 2) was used as a medium, and growth and characteristics were analyzed under a microscope from day 1 of culture. The culture temperature was 10 ° C, which is the most suitable temperature based on the results of Examples 1-3, and the whole amount of the medium was changed at weekly intervals. Roughness was incubated for one month at 500, 1,000, 2,000, 3,000 or 4,000 lux for each experimental group. After one month of incubation, the growth length of apoptosis was measured, and the formation of spores and growth state were observed during the culture.

The results are shown in FIG.

As shown in FIG. 8, the aposomes of the 500 lux test group were grown to 155 μm, the aposomes of the 1,000 lux test group were 450 μm, the aposomes of the 2,000 lux test group were 578 μm, and the aposomes of the 3,000 lux test group were 369 μm, respectively. The 4,000 Lux test group died. Therefore, it was confirmed that the most suitable illuminance for the growth of Gadashishima residents was 2,000 lux.

1-5: Kadashima  Production of seedlings

In order to produce a seedling of Gaedamashima, based on the results of the above Examples 1-3 and 1-4, the strainer was incubated to the seedling via the spores and apoptoses using the seedling frame as follows.

Temperature was 10 ~ 13 ℃, roughness was about 2,000 lux, PESI medium was added to 20 ㎖ per 1L of seawater, the return was carried out once a week. As for the seedlings, 18 ply Guraron yarn was used and 10 seedlings per ton were incubated for 40 days.

As a result of culture, spores were formed on about 10 days of culture, and about 400 μm of apoptoids were formed after 20 days, and a seedling of 1-2 cm was obtained one month later. In addition, in the case of political culture, the growth of the seedlings started to slow down slowly at 5 ~ 10 cm, and in the indoor culture, the initial growth was more active at low light conditions than at high light levels, and various temperature conditions were required depending on the growth stage. I could see that.

9 shows a picture of the seedlings cultured in glass, and a picture of the seedlings artificially produced using a seedling line in Figure 10, Figure 11 shows the ropes living creatures attached to only rocks such as seaweed, kelp, 톳, mabanban Photographs of the seedlings implanted in the parent rope, a rope installed at a certain depth below the surface of the water, were shown. In the parent's room, the thread, which is seedlings grown in the indoor tank, is wrapped around the seed line, or the string is cut short and inserted into the parent's seat at regular intervals so that the shoots grow. Moss hangs at intervals of 5 to 6 m, and the moss and the rope are connected by moss lines.

Example  2: Kadashima  Indoor aquaculture test

In order to elucidate the culture conditions of the seedling seedlings, aquaculture tests were conducted for mass production of seedlings using the seedlings of the working type produced in Example 1-5.

The cultured seedlings (5.7 ± 2.5 mm) were transplanted into 35 ± 8.9 individuals / 10 cm 2 on a previously prepared 5 m-long subterranean and cultured by depth (5, 10, 20, 30 m). At 6 months after the start of the culture, the leaf length, leaf width, and weight of the seedlings were measured to determine the extent of growth, and the habitat density was measured.

The indoor adaptation test and the growth test procedure at 30 m of the Kadashima seedlings are shown in FIG. 12, and the results of measuring the growth are shown in Table 3 and FIG. 13.

Habitat Density of Kadashima by the depth division Format Density (Individual / 10㎠) Depth (m) 5 10 20 50 Start training 35 ± 8.9 35 ± 8.9 35 ± 8.9 35 ± 8.9 After 6 months 12.4 ± 3.7 9.1 ± 4.5 8.1 ± 3.6 5.6 ± 4.7

As shown in Table 1 and FIG. 13, at 5 m depth, the leaves were grown to 120.4 ± 21.5 cm, leaf width 20.5 ± 6.5 cm, weight 54.7 ± 5.1 g, and the density was 12.4 ± 3.7 individuals / cm 2, depth 10 In m, the leaves were grown to 115.4 ± 25.9 ㎝, leaf width 18.6 ± 7.6 ㎝, weight 50.9 ± 7.9 g and habitat density 9.1 ± 4.5 individuals / cm 2, and at 20 m depth, 116 ± 20.8 ㎝, leaf width 17.5 ± 6.5 ㎝, weight 49.2 It was grown to ± 11.9 g and habitat density 8.1 ± 3.6 individuals / cm 2. At 30 m depth, the leaves were grown to 109 ± 319.5 mm, leaf width 19.6 ± 7.5 cm, weight 53.8 ± 6.9 g and habitat density 5.6 ± 4.7 individuals / cm 2.

Therefore, growth and habitat density at 5 m depth were found to be the best. At the start of the seedling size, 5 m depth is the most contiguous environment. From July, when the warm water temperature is high, it is expected to grow to 20 to 30 m depth to grow perennial.

Example  3: Kadashima  form

Based on the result of the said Example 1 and Example 2, Kadashima was cultured as follows.

First, a fish farm was established for farming Kadashima. Taking into account the characteristics of the sea in the East Sea, the anchor made two tons of square concrete x 3 pieces one by one and combined these two bundles to create anchors. Anchor strings were used for PE ropes 32 mm, and the rope was used for PE ropes 30 mm. The spacing of the cords should be more than 40 m. The main buoy had a PVC diameter of 520 mm and the auxiliary rich man had a PVC diameter of 330 mm. Kyojo, a seedling attached to a seedling, was wound around two or one layers on a hydrant (18 mm in diameter) to produce a hydrant of about 5 m in length, and was cultured at intervals of 3 to 4 m. At the time of farming, anchors were hung at the bottom so that they were always facing downward. The fish farmed at a depth of 5 m until June and 20 to 30 m after July, and other procedures necessary for kelp farming such as weaning, feeding, and pest control were carried out in the usual manner.

Fig. 14 shows the farming process of the Kadashima Island, and Fig. 15 shows the farm design.

The artificial seedling production method of Gaedamashima of the present invention is a method of collecting spores from Gaedamashima seedlings whose maturity has been determined to be mature by tissue cutting, and cultivating seedlings, by incubating the spores at a temperature and roughness suitable for growth. Enhances the apoptotic activity of the seed, resulting in artificial production of seedlings. In addition, artificially produced seedlings can be used for the large-scale farming of Kadashima Island, which will gradually decrease in habitat to restore endangered Kadashima colony, diversify seaweed farming varieties, and spread farming technology to contribute to the increase of income of fishers. Can be. In addition, it can be used as a source of medicine and health food raw materials that create high value through mass production of Kadashima.

Claims (14)

A seedling step in which a mature Gadashi-machi imitation formed with a platypus plaque is cut out, and a cutaneous plaque-forming site is cut out from the replica, and subjected to refrigeration and drying at 11-13 hours and at 3.5-4.5 ° C for the platypus; And Obtaining seedlings by culturing the yuju using 18 plywood Guraronsa as a seedling Artificial seedling production method of Gadamashima ( Kjellaniella crassifolia ) comprising a. The method of claim 1, wherein the step of culturing the strainer to obtain seedlings Culturing the strainer to obtain a mate; Culturing the spores to obtain apoptosis; And Culturing the apoptosis to obtain seedlings Artificial seedling production method of Gadamashima ( Kjellaniella crassifolia ) comprising a. delete The method for producing artificial seedlings of Kadashima, according to claim 1, wherein the refrigeration shade is carried out for 12 hours. delete The artificial seedling production method of dog shimashima, according to claim 1, wherein the refrigeration shade is performed at 4 ° C. The method of producing artificial seedlings of Kadashima, according to claim 2, wherein the apoptotic culture temperature is 10 to 13 ° C. The method of producing artificial seedlings of Gadamashima Island according to claim 2, wherein the culture temperature of the strainer and the spore is 10 ° C. The method of producing artificial seedlings of Kaedashima Island according to claim 1, wherein the strainer is incubated at an illuminance of 1500 to 2500 lux. 10. The method for producing artificial seedlings of dog larvae according to claim 9, wherein the incubator is incubated at a roughness of 2000 lux. The method of producing artificial seedlings of Gadamashima Island according to claim 1, wherein the fermenter is cultured for 35 to 45 days. 12. The method of producing artificial seedlings of Kadashima, according to claim 11, wherein the incubator is cultured for 40 days. Implanting the artificial seedlings produced by the method of claim 1 in the water drop yeon and planting them at a depth of 4 to 6 m at a first stage; And Secondary farming of the cultured seedlings at a depth of 20-30 m Kadashima farming method comprising a. The method of culture in Kadashima according to claim 13, wherein the seedling is installed at a depth of 5 m in the first aquaculture stage.

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