KR20190124326A - 유도 다능성 줄기 세포 또는 분화된 세포를 제조하기 위한 자동화 시스템 - Google Patents
유도 다능성 줄기 세포 또는 분화된 세포를 제조하기 위한 자동화 시스템 Download PDFInfo
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Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014013152B1 (pt) | 2011-12-01 | 2021-08-31 | New York Stem Cell Foundation, Inc | Sistema automatizado para geração de células-tronco pluripotentes induzidas (ipscs) |
| US10428309B2 (en) | 2011-12-01 | 2019-10-01 | New York Stem Cell Foundation, Inc. | Systems and methods for producing stem cells and differentiated cells |
| WO2014201412A1 (en) * | 2013-06-13 | 2014-12-18 | The New York Stem Cell Foundation Inc. | Pluripotent stem cells derived from non-cryoprotected frozen tissue and methods for making and using the same |
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| US10202568B2 (en) * | 2013-08-12 | 2019-02-12 | Invivosciences Inc. | Automated cell culture system and method |
| US20160222355A1 (en) * | 2014-12-26 | 2016-08-04 | New York Stem Cell Foundation, Inc. | Systems and methods for producing stem cells differentiated cells, and genetically edited cells |
| US9828634B2 (en) | 2015-01-22 | 2017-11-28 | Regenerative Medical Solutions, Inc. | Markers for differentiation of stem cells into differentiated cell populations |
| CN108138130A (zh) | 2015-08-31 | 2018-06-08 | 爱平世股份有限公司 | 多能干细胞制造系统和生产诱导多能干细胞的方法 |
| CN105567642B (zh) * | 2016-02-01 | 2019-07-12 | 中国科学院生物物理研究所 | 一种着色性干皮病人多能干细胞的制备方法 |
| JP7235507B2 (ja) | 2016-03-03 | 2023-03-08 | ニューヨーク ステム セル ファウンデーション インコーポレイテッド | 多能性幹細胞由来のミクログリアならびにその作製および使用方法 |
| EP3487982A1 (en) * | 2016-07-21 | 2019-05-29 | Celyad | Method and apparatus for automated independent parallel batch-processing of cells |
| US10373109B2 (en) | 2016-08-04 | 2019-08-06 | Fanuc Corporation | System and method for iPS cell bank using media |
| US10354218B2 (en) | 2016-08-04 | 2019-07-16 | Fanuc Corporation | System and method for iPS cell bank using internet technology |
| US11259520B2 (en) | 2016-08-04 | 2022-03-01 | Fanuc Corporation | Stem cell manufacturing system, stem cell information management system, cell transport apparatus, and stem cell frozen storage apparatus |
| AU2017362475B2 (en) | 2016-11-18 | 2023-08-10 | New York Stem Cell Foundation, Inc. | Microfluidic system and method of use thereof |
| KR102318710B1 (ko) | 2017-01-20 | 2021-10-27 | 후지필름 가부시키가이샤 | 세포 배양 장치 및 세포 배양 방법 |
| JP6998018B2 (ja) * | 2017-02-24 | 2022-01-18 | 剛士 田邊 | 細胞処理装置、浮遊培養器、及び幹細胞の誘導方法 |
| CN110392733B (zh) * | 2017-02-27 | 2021-02-26 | 田边刚士 | 体细胞制造系统 |
| WO2018154791A1 (ja) * | 2017-02-27 | 2018-08-30 | 剛士 田邊 | 細胞処理システム及び細胞処理装置 |
| LT3645719T (lt) * | 2017-06-30 | 2022-05-25 | Inscripta, Inc. | Automatinio ląstelių apdorojimo būdai, moduliai, priemonės ir sistemos |
| GB2564437B (en) | 2017-07-10 | 2020-11-04 | Univ Brunel | Method of testing a gene therapy vector using induced pluripotent stem cells |
| KR102296580B1 (ko) | 2017-09-01 | 2021-09-02 | 론자 워커스빌 아이엔씨. | 엔드-투-엔드 세포 요법의 자동화 |
| US10443074B2 (en) * | 2017-09-30 | 2019-10-15 | Inscripta, Inc. | Modification of cells by introduction of exogenous material |
| CN112292445A (zh) * | 2018-06-13 | 2021-01-29 | 富士胶片株式会社 | 信息处理装置、导出方法及导出程序 |
| JP6530577B1 (ja) * | 2019-03-20 | 2019-06-12 | 剛士 田邊 | 細胞処理システム及び細胞処理装置 |
| JP7090337B2 (ja) * | 2019-03-20 | 2022-06-24 | アイ ピース，インコーポレイテッド | 細胞処理システム及び細胞処理装置 |
| JP6530874B1 (ja) * | 2019-03-20 | 2019-06-12 | 剛士 田邊 | 細胞処理システム及び細胞処理装置 |
| CA3156826A1 (en) * | 2019-10-04 | 2021-04-08 | New York Stem Cell Foundation, Inc. | Imaging system and method of use thereof |
| CN115087730A (zh) * | 2019-11-28 | 2022-09-20 | 香港大学 | 间充质基质细胞作为ipsc诱导的重编程来源 |
| CN117642496A (zh) * | 2021-06-17 | 2024-03-01 | 基因泰克公司 | 自动化细胞培养系统 |
| US12385002B1 (en) * | 2022-01-21 | 2025-08-12 | Sahar M. H. Jaffal | Automated primary cells' triturator |
| CN114606198A (zh) * | 2022-03-25 | 2022-06-10 | 深圳临研医学有限公司 | 成骨发育不全诱导多能干细胞及其构建方法 |
| WO2024020572A1 (en) | 2022-07-22 | 2024-01-25 | New York Stem Cell Foundation, Inc. | Methods and compositions for the treatment of ptsd |
| AU2024208789A1 (en) * | 2023-01-11 | 2025-07-24 | Regeneron Pharmaceuticals, Inc. | Systems and methods for a regulatory-compliant automated assay |
| WO2024228384A1 (ja) * | 2023-04-30 | 2024-11-07 | 国立大学法人北海道大学 | 免疫寛容誘導用組成物 |
| JP2025064611A (ja) * | 2023-10-06 | 2025-04-17 | 住友化学株式会社 | 細胞チップ |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US5994619A (en) | 1996-04-01 | 1999-11-30 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Production of chimeric bovine or porcine animals using cultured inner cell mass cells |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| EP0996736A1 (en) | 1997-08-11 | 2000-05-03 | Chiron Corporation | Methods for genetically modifying t cells |
| US8026096B1 (en) * | 1998-10-08 | 2011-09-27 | Protein Sciences Corporation | In vivo active erythropoietin produced in insect cells |
| US20110171185A1 (en) * | 1999-06-30 | 2011-07-14 | Klimanskaya Irina V | Genetically intact induced pluripotent cells or transdifferentiated cells and methods for the production thereof |
| US6429013B1 (en) | 1999-08-19 | 2002-08-06 | Artecel Science, Inc. | Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair |
| US6959438B2 (en) | 2000-12-06 | 2005-10-25 | Microsoft Corporation | Interface and related methods for dynamically generating a filter graph in a development system |
| US8492140B2 (en) * | 2002-04-08 | 2013-07-23 | Octane Biotech Inc. | Automated tissue engineering system |
| EP1445320A1 (en) | 2003-02-05 | 2004-08-11 | ARTEMIS Pharmaceuticals GmbH | Automated gene-targeting using non-toxic detectable markers |
| CA2560052A1 (en) * | 2004-03-19 | 2005-10-13 | Macropore Biosurgery Inc. | Cell carrier and cell carrier containment devices containing regenerative cells |
| US20070238175A1 (en) | 2006-04-06 | 2007-10-11 | Chi Alfred L | Standardization of processes for culturing primary cells |
| GB0704406D0 (en) | 2007-03-07 | 2007-04-18 | Stem Cell Sciences Uk Ltd | Automated culture of stem cells |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| DK2173863T3 (en) * | 2007-06-29 | 2019-01-21 | Fujifilm Cellular Dynamics Inc | Automated method and apparatus for embryonic stem cell culture |
| WO2009023246A2 (en) * | 2007-08-14 | 2009-02-19 | Wake Forest University Health Sciences | Pluripotent adult stem cells |
| EP2315829B1 (en) | 2008-08-08 | 2015-02-11 | Mayo Foundation For Medical Education And Research | Induced pluripotent stem cells |
| US9045737B2 (en) | 2008-12-13 | 2015-06-02 | Dnamicroarray, Inc. | Artificial three-dimensional microenvironment niche culture |
| ES2645869T3 (es) | 2008-12-17 | 2017-12-11 | The Scripps Research Institute | Generación y mantenimiento de células madre |
| CA2753208C (en) * | 2009-02-20 | 2018-08-21 | Cellular Dynamics International, Inc. | Methods and compositions for the differentiation of stem cells |
| EP2401364B1 (en) * | 2009-02-27 | 2015-04-22 | Cellular Dynamics International, Inc. | Differentiation of pluripotent cells |
| CN101580816B (zh) | 2009-04-23 | 2012-02-29 | 中国科学院广州生物医药与健康研究院 | 诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法 |
| US20110020814A1 (en) * | 2009-06-05 | 2011-01-27 | Ipierian, Inc. | Methods and compositions for selection of stem cells |
| DK2548950T3 (en) * | 2009-06-05 | 2017-12-11 | Cellular Dynamics Int Inc | Reprogramming of T cells and hematopoietic cells |
| US20120252697A1 (en) * | 2009-09-01 | 2012-10-04 | Mcmaster University | Transformed human pluripotent stem cells and associated methods |
| WO2011037301A1 (ko) * | 2009-09-22 | 2011-03-31 | 서울대학교병원 | 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포 |
| EP3072961A1 (en) | 2010-04-16 | 2016-09-28 | Children's Medical Center Corporation | Sustained polypeptide expression from synthetic, modified rnas and uses thereof |
| US20110306516A1 (en) | 2010-06-15 | 2011-12-15 | The New York Stem Cell Foundation | Methods for producing induced pluripotent stem cells |
| KR101975688B1 (ko) | 2010-12-22 | 2019-05-07 | 페이트 세러퓨틱스, 인코포레이티드 | 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼 |
| EP2481795A1 (en) | 2011-01-28 | 2012-08-01 | Universität für Bodenkultur Wien | Method of generating induced pluripotent stem cells and differentiated cells |
| BR112014013152B1 (pt) * | 2011-12-01 | 2021-08-31 | New York Stem Cell Foundation, Inc | Sistema automatizado para geração de células-tronco pluripotentes induzidas (ipscs) |
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