KR20190079075A - 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물 - Google Patents

신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물 Download PDF

Info

Publication number
KR20190079075A
KR20190079075A KR1020170181009A KR20170181009A KR20190079075A KR 20190079075 A KR20190079075 A KR 20190079075A KR 1020170181009 A KR1020170181009 A KR 1020170181009A KR 20170181009 A KR20170181009 A KR 20170181009A KR 20190079075 A KR20190079075 A KR 20190079075A
Authority
KR
South Korea
Prior art keywords
bacteriophage
present
food
cronobacter sakazakii
composition
Prior art date
Application number
KR1020170181009A
Other languages
English (en)
Other versions
KR102066920B1 (ko
Inventor
연영은
김영주
한범구
김현일
박철세
Original Assignee
주식회사 옵티팜
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 옵티팜 filed Critical 주식회사 옵티팜
Priority to KR1020170181009A priority Critical patent/KR102066920B1/ko
Publication of KR20190079075A publication Critical patent/KR20190079075A/ko
Application granted granted Critical
Publication of KR102066920B1 publication Critical patent/KR102066920B1/ko

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/195—Antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/34635—Antibiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/381—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00031—Uses of virus other than therapeutic or vaccine, e.g. disinfectant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00032—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)

Abstract

본 발명은 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 사멸능을 갖는 신규 박테리오파지 CS3, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물, 상기 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는 크로노박터 사카자키균이 제거된 식품 제조 방법 및 상기 박테리오파지를 인간을 제외한 개체에 투여하는 단계를 포함하는 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다. 본 발명의 박테리오파지 CS3를 이용한 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 크로노박터 사카자키에 대한 특이성이 매우 높고, 상기 박테리오파지 CS3는 상기 크로노박터 사카자키에 감염하여 크로노박터 사카자키 균주 내에서 증식함으로써 용균 활성이 우수하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 CS3는 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 의한 인간을 제외한 개체의 감염성 질병의 예방 또는 치료방법, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물 분야에서 다양하게 사용될 수 있다.

Description

신규한 크로노박터 사카자키 특이 박테리오파지 CS3 및 이를 포함하는 항균 조성물{Novel Cronobacter sakazakii specific bacteriophage CS3 and antibacterial composition comprising the same}
본 발명은 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 사멸능을 갖는 신규 박테리오파지 CS3, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물, 상기 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는 크로노박터 사카자키균이 제거된 식품 제조 방법 및 상기 박테리오파지를 인간을 제외한 개체에 투여하는 단계를 포함하는 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.
크로노박터 사카자키(Cronobacter sakazakii)는 장내세균과에 속하는 그람 음성의 간균으로 포자를 형성하지 않는 통성혐기성균이다. 토양이나 물 등의 다양한 환경과 육류, 채소류, 유제품에 이르기까지 광범위한 식품군에서 검출되고 있다. 모든 연령대에서 질환을 일으킬 수 있으나 면역력이 약한 신생아 및 저체중아에 감염될 위험이 높으며 발생 빈도는 낮으나 장염, 패혈증, 수막염 등을 유발하는 고위험균이다. 1958년 영국에서 최초로 사카자키에 의한 감염 보고가 있었고 그 이후 연구자들에 의해 이유식과 분말우유가 신생아의 사카자키균 감염의 원인으로 밝혀졌다.
크로노박터 사카자키는 건조 및 훈제, 튀김 제품류, 즉석 식품과 같은 가공 식품뿐만 아니라 동물이나 식물성 원료의 산물 또는 신선한 제품에서 검출된다. 축산물의 경우 원료 동물의 장내미생물에 의해서 추가로 오염될 수 있다. 식품의 가공 과정에서 물, 토양, 먼지 등 환경적인 요인을 처리하지 않은 모든 원료는 잠재적인 오염 물질이며, 부적절한 위생 관리로 인하여 작업자와 장비를 통해 사카자키균의 오염의 가능성이 있다. 광범위한 식품에서 검출될 가능성이 있기 때문에 위생 관리에 대한 규칙을 준수해야 한다. 건강한 사람에게는 영향을 크게 미치지 않지만 신생아, 노인 및 면역 결핍 환자의 경우 심각한 질병과 사망을 유발할 수 있는데 특히 위장 계통과 신경계에 영향을 미치는 것으로 밝혀졌다.
사카자키균에 의한 질병은 모든 연령층에서 발생할 수 있으나, 주로 28일 이하의 신생아에 관해 보고된 사례들이 많다. 대략적으로 감염된 신생아수의 반 정도가 출생체중이 2 kg 이하였고, 이 중 3분의 2정도가 37주 이하인 조산아였다. 또한 면역력이 약한 신생아가 감염에 더 민감한 경향을 보였으며, 감염 시 20-40%의 사망률을 보이고 있다. 이러한 사카자키균 감염 발생은 오염된 유아용 조제분유가 원인으로 감염 시 패혈증, 뇌수막염, 괴사성 장염 등을 유발한다. 뇌수막염을 앓은 뒤에 살아남은 대부분의 환자는 수두증, 사지 마비, 발달 지체 등의 심각한 신경계 후유증을 앓고 있다. 또한 괴사성 장염이 발생한 신생아들의 29%에서 사카자키균이 발견되었다는 연구가 보고되었다. 크로노박터 사카자키는 열에 매우 안정한 것으로 나타나며 분유 제조 시 건조 공정을 거쳐도 생육이 가능하고, 건조한 조제분유에서도 스스로 캡슐화하여 분유의 유통기한인 24개월 동안 장기 지속성을 나타내기 때문에 유아용 조제분유의 안정성 관리가 요구된다.
한편, 박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지의 활용이 크게 주목을 받고 있다. 자연 친화적 방식의 선호로 인하여 박테리오파지에 대한 관심은 어느 때보다 높다고 할 수 있다. 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.
이에 본 발명자들은 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 용균활성을 갖는 박테리오파지를 분리하고, 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하여 상기 박테리오파지가 크로노박터 사카자키(Cronobacter sakazakii)를 선택적으로 사멸시킬 수 있음을 확인함으로써 본 발명을 완성하였다.
본 발명의 목적은 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 CS3를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 CS3를 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물을 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는 크로노박터 사카자키균이 제거된 식품 제조 방법을 제공하는 것이다.
본 발명의 또 다른 목적은 상기 박테리오파지 CS3를 인간을 제외한 개체에 투여하는 단계를 포함하는, 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 CS3를 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 항생용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 사료 첨가용 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 소독제를 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 세척제를 제공한다.
또한, 본 발명은 박테리오파지 CS3를 포함하는, 식품 조성물을 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는 크로노박터 사카자키균이 제거된 식품 제조 방법을 제공한다.
또한, 본 발명은 상기 박테리오파지 CS3를 인간을 제외한 개체에 투여하는 단계를 포함하는, 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 CS3를 이용한 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 크로노박터 사카자키에 대한 특이성이 매우 높고, 상기 박테리오파지 CS3는 상기 크로노박터 사카자키에 감염하여 크로노박터 사카자키 균주 내에서 증식함으로써 용균 활성이 우수하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 CS3는 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 의한 인간을 제외한 개체의 감염성 질병의 예방 또는 치료방법, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물 분야에서 다양하게 사용될 수 있다.
도 1은 박테리오파지 CS3의 크로노박터 사카자키에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 2는 박테리오파지 CS3 유전자 시퀀싱 분석 결과로 얻은 유전자 맵을 나타낸 도이다.
도 3은 전자 현미경을 통해 박테리오파지 CS3의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 4는 박테리오파지 CS3의 단백질 패턴을 SDS-PAGE를 통해 확인한 결과를 나타낸 도이다.
이하, 본 발명에 대해 상세히 설명한다.
본 발명은 크로노박터 사카자키에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 CS3를 제공한다.
본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 해당 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.
본 발명에서 특이적인 사멸능을 보이는 크로노박터 사카자키(Cronobacter sakazakii)는 장내세균과에 속하는 그람 음성의 간균으로 포자를 형성하지 않는 통성혐기성균이다. 토양이나 물 등의 다양한 환경과 육류, 채소류, 유제품에 이르기까지 광범위한 식품군에서 검출되는 균주이다. 모든 연령대에서 질환을 일으킬 수 있으나, 특히 면역력이 약한 신생아 및 저체중아에 감염될 위험이 높은 균주로 장염, 패혈증, 수막염 등을 유발하는 고위험균을 말한다.
본 발명의 박테리오파지 CS3의 전체 유전자의 크기는 149,257 bp 이며, GC함량이 51%, 전체 ORFs의 수는 279개이다. 상기 박테리오파지 CS3는 약 200-220 nm의 크기이며, 머리 길이는 80-100 nm이고 꼬리 길이는 100-120 nm이다. 정이십면체의 머리를 가지며 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속한다. 상기 박테리오파지 CS3의 단백질 패턴 분석 결과 56 kDa, 46 kDa, 34 kDa, 16 kDa, 그리고 14 kDa 크기의 단백질을 포함함을 확인하였다. 또한 상기 박테리오파지 CS3는 크로노박터 사카자키에 대한 우수한 감수성을 통한 크로노박터 사카자키 특이적인 용균 활성을 나타낸다.
본 발명의 박테리오파지 CS3는 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 CS3는 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.
크로노박터 사카자키 특이적 용균활성은 본 발명의 박테리오파지 CS3를 크로노박터 사카자키에 의한 감염성 질병의 예방 및 치료용 조성물 및 상기 박테리오파지 CS3를 유효성분으로 포함하는 다양한 제품에 적용이 가능하게 한다.
본 발명의 박테리오파지 CS3는 도축장, 도계장 및 농장 부근의 하수로부터 시료를 채취하여 분리한 박테리오파지로서, 본 발명자는 이를 박테리오파지 CS3로 명명하고 2017년 09월 13일에 한국미생물보존센터에 수탁번호 KFCC11736P로 기탁하였다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 항생용 조성물을 제공한다.
본 발명에서, 용어 항생용 조성물은 약제 형태로 개체에 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다.
본 발명의 박테리오파지 CS3는 기존 항생제에 비하여 크로노박터 사카자키에 대한 특이성이 매우 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 사료 첨가용 조성물을 제공한다.
축산, 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞어 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 CS3를 이용할 수 있다.
또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조 상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량%, 바람직하게는 0.1 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.
본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에(Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.
본 발명의 박테리오파지 CS3를 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.
본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.
또한, 본 발명은 박테리오파지 CS3를 포함하는, 음용수 첨가제를 제공한다.
본 발명의 음용수 첨가제는 상기 박테리오파지 CS3 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 크로노박터 사카자키의 숫자를 감소시킬 수 있는 효과가 있다.
본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 소독제를 제공한다.
크로노박터 사카자키에 특이적 사멸능을 갖는 본 발명의 박테리오파지 CS3를 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 세척제를 제공한다.
본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 특이적 사멸능을 가지므로, 크로노박터 사카자키에 노출되었거나 노출될 가능성이 있는 개체의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 포함하는, 식품 조성물을 제공한다.
본 명세서에서 식품이란 함은 영양소를 한 가지 또는 그 이상 함유하고 있는 천연물 또는 가공품을 의미하며, 바람직하게는 어느 정도의 가공 공정을 거쳐 직접 먹을 수 있는 상태가 된 것을 의미하며, 통상적인 의미이고, 상기 식품 조성물은 식품, 식품 첨가제, 건강 기능성 식품 및 음료를 모두 포함하는 의도이다.
본 발명의 조성물을 첨가할 수 있는 식품으로는 예를 들어, 각종 식품류, 음료, 껌, 캔디, 차, 비타민 복합제, 기능성 식품 등이 있다. 추가로, 본 발명에서 식품에는 특수영양식품(예, 조제유류, 조제분유, 영, 유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예, 라면류, 국수류 등), 건강보조식품, 조미식품(예, 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예, 스낵류), 유가공품(예, 발효유, 치즈 등), 기타 가공식품, 김치, 절임 식품(각종 김치류, 장아찌 등), 음료(예, 과실, 채소류 음료, 두유류, 발효음료류, 아이스크림류 등), 천연조미료(예, 라면 스프 등), 비타민 복합제, 알코올 음료, 주류 및 그 밖의 건강보조식품류를 포함하며, 바람직하게는 분유 또는 이유식에 포함될 수 있으나, 이에 한정되지 않는다. 상기 식품, 음료 또는 식품첨가제는 통상의 제조방법으로 제조될 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는 크로노박터 사카자키균이 제거된 식품 제조 방법을 제공한다.
본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 특이적인 사멸능을 가지므로, 본 발명의 박테리오파지를 사용하면 식품에 존재할 수 있는 크로노박터 사카자키균을 제거할 수 있어 분유 및 이유식을 포함한 식품의 제조공정에 이용될 수 있다.
또한, 본 발명은 상기 박테리오파지 CS3를 인간을 제외한 개체에 투여하는 단계를 포함하는, 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.
본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 특이적인 사멸능을 가지므로, 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있다.
본 발명에서, 크로노박터 사카자키에 의한 감염성 질병은 그 증상으로 패혈증, 뇌수막염, 괴사성 장염 등을 포함하나, 이에 제한되는 것은 아니며, 크로노박터 사카자키에 의한 2차 감염을 포함한다.
본 발명에서, 용어 "개체"는 크로노박터 사카자키에 의한 감염성 질병이 발병될 수 있는 모든 개체를 의미하는 것으로 인간을 제외한 포유동물, 가축류 또는 가금류를 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.
상기 용어 "가금류"는, 가축 중 조류에 속하는 동물을 총칭하여 이르는 개념으로, 예를 들어, 닭, 오리, 꿩, 메추리, 타조, 거위 또는 칠면조 등을 포함하나, 이에 제한되는 것은 아니다.
본 발명의 방법에서 상기 박테리오파지 CS3 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.
본 발명의 방법에서 상기 박테리오파지 CS3 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.
본 발명의 방법에 투여되는 상기 박테리오파지 CS3의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.
본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술 분야에서 통상적으로 사용되는 의미를 갖는 것이다.
이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.
실시예 1: 신규한 박테리오파지의 분리 및 배양
1.1 박테리오파지의 스크리닝 및 분리
크로노박터 사카자키에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 시료는 박테리오파지가 존재할 것으로 예상되는 양돈농장 부근의 하수와 토양 시료를 채취하였으며, 대상세균인 크로노박터 사카자키는 생물자원센터에서 분양(KCTC 2949)받은 것을 사용하였다.
구체적으로, 채취한 시료를 균질화 한 후 크로노박터 사카자키와 함께 35℃에서 하룻밤 동안 진탕배양 하였다. 상기 배양액을 4,000 rpm으로 20분간 원심분리한 후, 상등액을 공극(pore) 크기가 0.2 μm인 필터를 이용하여 여과하여 시료 여과액을 얻었다. 상기 여과액을 이용하여 용균반 분석을 통해 대상 시료에 크로노박터 사카자키를 특이적으로 용균할 수 있는 박테리오파지를 선별하였다.
다음으로 크로노박터 사카자키를 특이적으로 용균할 수 있는 박테리오파지를 스크리닝하기 위하여, 크로노박터 사카자키 150 μL를 취해서 멸균된 튜브에 넣고 3 mL의 BHI 탑 아가(top agar) [뇌심장침출액(뇌 침출 솔리드(solids) 12.5 g, 소 심장 침출 솔리드 5.0 g, 프로테오스펩톤 10.0 g, 글루코스 2.0 g, 소듐클로라이드 5 g, 인산나트륨 2.5 g/L) + 0.6% 아가]를 첨가하여 잘 혼합한 후, 미리 만들어 둔 2% BHI 아가 플레이트에 중층하였다. 실온에서 30분간 방치한 후 상기 시료 여과액을 각각 10 μL씩 점적하고, 다시 실온에서 30분간 방치한 후 35℃에서 24시간 동안 배양하여 박테리오파지의 유무를 확인하였다.
박테리오파지의 존재를 확인한 시료로부터 박테리오파지를 순수 분리하기 위해서 소프트 아가 오버레이(soft agar overlay)법을 이용하였다. 박테리오파지가 존재하는 것으로 확인된 시료를 식염수로 적절히 희석하여 각 희석단의 시료 희석액 100 μL를 취하고, 대상 세균(크로노박터 사카자키) 배양액 150 μL에 혼합한 후 0.6% BHI 탑 아가 3 mL을 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% BHI 아가 플레이트에 중층하여 방치한 후 35℃에서 24시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후, 이를 선택하여 400 μL의 SM 완충액(NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-HCl(pH 7.5) 50 mL)에 현탁하고, 실온에서 4시간 동안 방치하였다. 상기 박테리오파지액 100 μL를 0.6% BHI 탑 아가와 세균 배양액 500 μL와 혼합한 뒤 150 mm 지름의 BHI 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, BHI 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간 동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 3,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 1에 나타내었다.
도 1에 나타낸 바와 같이, 5-7 mm의 크기를 가지는 용균반을 형성하는 박테리오파지를 확인하였고, 상기 박테리오파지는 크로노박터 사카자키에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.
1.2 박테리오파지의 대량 배양 및 정제
상기 실시예　1.1에서 확인한 크로노박터 사카자키 특이적 박테리오파지를 대량 배양하기 위하여, 1.1에서 회수한 최종 박테리오파지 배양액을 1.0×109 PFU/mL로 희석한 후 전배양된 크로노박터 사카자키 1.0×109 CFU/mL와 혼합하여 MOI(Multiplicity of infection)가 1이 되도록 하여 35℃에서 100 rpm으로 18시간 동안 배양하였다. 배양이 종료된 후 배양액 전량을 6,000 rpm으로 30분간 원심분리하여 상등액을 수득하였고, 이를 0.2 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다. 분리된 박테리오파지를 박테리오파지 CS3(Bacteriophage CS3)로 명명하고, 2017년 09월 13일 한국미생물보존센터(Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하고 수탁번호 KFCC11736P를 부여받았다.
실시예 2: 박테리오파지 CS3의 전체 유전자 분석
박테리오파지 CS3의 전체 유전자를 분석하기 위해, 박테리오파지 CS3 배양액에 20 mM EDTA, 50 μg/mL의 프로테나아제 K, 0.5%(w/v) SDS를 가한 후, 50℃에서 2시간 동안 정치하였다. 동량의 페놀-클로로포름-이소프로필 알코올(25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액을 회수하였다. 회수된 상등액을 동일 부피의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액을 회수하고, 상기 상등액에 3M 소듐 아세테이트를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 2시간 동안 정치하였다. 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리하여 침전물을 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA 펠렛을 회수하였다. 회수한 펠렛을 최종 부피가 50 μL가 되도록 TE 완충액(Tris-EDTA, pH8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 PacBioRSII 를 이용하여 유전자 시퀀싱을 수행하였다(마크로젠, 한국). 상기 시퀀싱 분석을 수행한 결과로 얻은 박테리오파지 CS3의 유전자 맵을 도 2에 나타내었다.
도 2에 나타낸 바와 같이, 박테리오파지 CS3의 전체 유전자의 크기는 149,257 bp를 가지며, GC함량이 51%, 전체 ORFs의 수는 279개임을 확인하였다. 상기 분석한 박테리오파지 CS3의 전체 유전자 서열을 서열번호 1로 나타내었다.
실시예 3: 박테리오파지 CS3의 형태학적 특성
박테리오파지 CS3의 형태학적 특성을 알아보기 위해, 배양액을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 Carbon-coated mica plate(ca. 2.5 x 2.5 mm)에 적가한 후, 10분간 적응시켜 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30-60초간 염색하고 건조시킨 후, 투과전자현미경(Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 관찰하였다. 그 결과를 도 3에 나타내었다.
도 3에 나타낸 바와 같이, 박테리오파지 CS3는 약 크기는 200-220 nm이며, 머리 길이는 80-100 nm이고 꼬리 길이는 100-120 nm인 것을 확인하였다. 또한, 상기 박테리오파지는 정이십면체의 머리를 가지며 꼬리가 있는 형태인 마이오비리대(Myoviridae)에 속함을 확인하였다.
실시예 4: 박테리오파지 CS3의 단백질 패턴 분석
박테리오파지 CS3의 단백질 패턴을 분석하기 위해, 1.0×1010 PFU/mL로 준비된 박테리오파지 CS3 40 μL를 10 μL의 5X 로딩 완충액과 혼합하고, 10분간 끓인 다음 12.5% SDS-PAGE를 수행하였다. SDS-PAGE 수행 후 단백질 전기영동 결과를 도 4에 나타내었다.
도 4에 나타낸 바와 같이, 박테리오파지 CS3는 56 kDa, 46 kDa, 34 kDa, 16 kDa, 그리고 14 kDa 크기의 단백질을 포함함을 확인하였다.
실시예 5: 박테리오파지 CS3의 다양한 세균에 대한 감염성 조사
크로노박터 사카자키를 포함한 다른 다양한 세균에 대해서 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양받은 균주를 대상으로 소프트 아가 오버레이법을 통해서 감염성을 확인하였으며, 그 결과를 표 1에 나타내었다.
Figure pat00001
(+; 용균활성 있음, -; 용균활성 없음)
상기 표 1에 나타낸 바와 같이, 본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 대해 높은 감수성을 가지는 것을 확인하였다. 또한, 크로노박터 사카자키 외에 다른 속인 Salmonella, Bacillus, Listeria 종 등의 균주에 대해서는 용균활성이 없는 것을 확인하였다.
따라서, 본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 특이적으로 용균활성을 나타내는 박테리오파지임을 확인하였다.
상기 실험으로부터 박테리오파지 CS3는 크로노박터 사카자키에 대한 특이성이 매우 높고, 크로노박터 사카자키 내에서 증식함으로써 용균 활성이 우수하다는 장점을 가지고 있음을 확인하였다. 또한 본 발명의 박테리오파지 CS3는 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 CS3는 크로노박터 사카자키에 의한 감염성 질병의 인간을 제외한 개체의 예방 또는 치료방법, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 세척제 또는 식품 조성물 분야에서 다양하게 사용될 수 있다.
비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한 첨부된 청구 범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.
이하 본 발명의 박테리오파지 CS3를 포함하는, 감염성 질병의 예방 또는 치료용 약학적 조성물 및 식품 조성물의 제제예를 설명하나, 본 발명을 한정하고자 함이 아니고 단지 이를 구체적으로 설명하고자 함이다.
제제예 1: 의약품의 제조
1.1 주사제의 제조
박테리오파지 CS3 1 × 106 PFU/mL
주사용 멸균 증류수 적량
pH 조절제 적량
통상의 주사제의 제조방법에 따라 1앰플당(2 mL) 상기의 성분 함량으로 제조한다.
1.2 액제의 제조
박테리오파지 CS3 1 × 106 PFU/mL
설탕 20 g
이성화당 20 g
레몬향 적량
정제수를 가하여 전체 1,000 mL로 맞추었다. 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조한다.
1.3 정제의 제조
박테리오파지 CS3 1 × 106 PFU/mL
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.
제제예 2. 식품 제제의 제조
1. 건강식품의 제조
박테리오파지 CS3 100 ml
비타민 혼합물 적량
비타민 A 아세테이트 70 g
비타민 E 1.0 mg
비타민 B1 0.13 mg
비타민 B2 0.15 mg
비타민 B6 0.5 mg
비타민 B12 0.2 g
비타민 C 10 mg
비오틴 10 g
니코틴산아미드 1.7 mg
엽산 50 g
판토텐산 칼슘 0.5 mg
무기질 혼합물 적량
황산제1철 1.75 mg
산화아연 0.82 mg
탄산마그네슘 25.3 mg
제1인산칼륨 15 mg
제2인산칼슘 55 mg
구연산칼륨 90 mg
탄산칼슘 100 mg
염화마그네슘 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 건강식품에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강식품 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강식품 조성물 제조에 사용할 수 있다.
2. 건강음료의 제조
박테리오파지 CS3 100 ml
비타민 C 15 g
비타민 E(분말) 100 g
젖산철 19.75 g
산화아연 3.5 g
니코틴산아미드 3.5 g
비타민 A 0.2 g
비타민 B1 0.25 g
비타민 B2 0.3g
물 정량
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1시간 동안 85℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2 리터 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 건강음료 조성물 제조에 사용한다.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만 수요계층이나, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.
한국미생물보존센터(국내) KFCC11736P 20170913
SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Cronobacter sakazakii specific bacteriophage CS3 and antibacterial composition comprising the same <130> 1-62P <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 149257 <212> DNA <213> direct input <400> 1 ggcatggcga atcgggggtg gggaggggcc tcgttatttt ccccaaatat ccttatgaat 60 caatgactta gcccctctca acttttcttc aatcggaccg ggtaggtcct cgctttacgt 120 gatcatctcc acaaaatgtc taccatcttg cccatcttta cacttgtcct attgactcac 180 ccggcctgat gtgcattcgt cccacaatac ctatttaaca taatacacct tatacgcact 240 cgggataatg ataatcattc ccaacacgtg gagaggcccg ccagcactgg cgtagatgag 300 aatgagtctc actcaccttg tggcatgata atgagaatga tttgcattcg ccctttgaga 360 atggttgtcg gatgagaacc attatcgttt acgcaaaaag aaaaaaaaaa ttatttttcg 420 cttgacacga agagcgaatt ttgccaggca aaagtcctgt agatgattac ttttcgcaca 480 ataaaggccc tcccctctca ggccctccgg cccgtaccgg gtccgcctgt ttacgtaact 540 atgcagataa aacacgattt tatgcagatg agaataattc gtatttgtgc ctgtaggcac 600 taaaaagccc ttgacaatgg cgattttatt actacgtaat gaccaataga aaacaggtga 660 tagcggtcat cccttgaccg tgggaacgtg agcacaatcc tttgcgagct agcaaagggg 720 gtgatccgtt acctgttttc tattggtcgc gcctacggcg cgccctccct tgatccgtgg 780 gaacgtaaaa ccgtgttttg tcagaagtca ataaaaagtt aagttattga tagtaaatga 840 gaatcattat cgcattatgc agataaatga ataaatagtg tcaagttatg taaagaaaat 900 ggatgattcg ccgattcggc ctgatacacg tttgcagata gaagtcaata ggggctaaaa 960 cgcgctgtaa ggccctatgc gggccgctac cctcaaccct ggttagtggg ttaggggcgc 1020 tgttattcaa ttatgcactt ttgagggtga aaattaatgg ttgatttcca caatgttaaa 1080 tgataagggg ctataggctg ggggagtcgg accgataccg ggtcgggcct gctggcttgc 1140 cacgtttggg acaaaagaaa agcccgccgg ggtggcgggc ctgtttggct gaatggtgtt 1200 agttaaggca ggtaaccagg gcaggcagtg ccagcgccag atcataacgg tttaaggatt 1260 tacgacgata agccaggcgg gccgttgcca gtgtagcgcc gaaagcgttt aaaaatgttg 1320 tgaccatttg atcgggtccg agtacagcgg ataaaatcca gttgtttacg gtatcgccag 1380 aaaacgcgat gatgaccgcc gccagggtcc aaagtggaag attgatttta aaaaggcggt 1440 tcattatggc ttaatctcgt tttgttgggt gagatcgcat tatggggcct ttcggcccct 1500 gagtcaatat tattttttgt ttttgtactc ttcgatccag cgggtgaaga tttcagcttg 1560 cttttctgcc gctgccgctg ttttgtgttg catcacttca acatgccaac aatcggcaag 1620 ggtgaagcct gaagcgtgag caacccacgg ataagacgga tcagattctt tgtaagacgc 1680 caaacgggcc ggatcttcgt ggaatacgta gccctctagc tgtgcggcgg tgtattcgtt 1740 gccgccgttg cctaacttcg cccattcgtt atggcttgac aggtagcgat ccagcatttc 1800 gaaaaattta gatttggtca ttttttacgg tctccagtgt ttttagtggt ttgttgcttg 1860 agtgggtatt atggggcctt tcggccccct tgtcaacgat taatttaaat ccagattcag 1920 ggccagatct accagggcgg cgatctcctc ttctgttgcg atgcggccca ctacccagcg 1980 gatatagatt ttaatcaata atgctttgtt gcttgcgtag cccggatgtg ataacagatc 2040 tttacggcgt aatttgtcat ctgttgcgag ggtcatcacg ccgcctttgt actcaatgga 2100 gatctgcaaa ccgctttcaa aggtcgccat aaaatagttt tcaaaggtaa cagtttcaac 2160 gtgtaacttt gcgccgttta cgtgctgttg catttcgaac gaacgatcat acccgaaagc 2220 atttgcctgt tcgtcgcgga tgtagtagcg gttgaaattg ttcaggttca ttttacggtc 2280 tcctctctgt gggccgtcgc atcattgccg ccctacgtga taaataatag cacaatggcg 2340 gatcgatgca atagggagaa ttgtaaagtt ttgtcaacgt aaacccggct ttcagccctt 2400 caggtctaac ataaaaattt tacgtttgca agggcaaaaa gtgcgaaagt ccgatttcgg 2460 gagatctata tttttatgat gtaggatacc gggtaagggg aaaacggctt aaaacgcgat 2520 ttagagcgtt tggcgggcat gtacatttat acaggagtaa gaaataaatt tgacaacggt 2580 ttgaaagtat gtatttgatt ttgttggaaa tgataaccat tctcatttgc ccggttcggc 2640 ccaggtccgg tccgcctccc tgaatagcgg gcaggtccgg tgtttgtctc cccctgcatc 2700 aggtgccatc gcatgacata agaataacaa aaaaggggcg aacaagtcaa cccctgtaaa 2760 cactgttaat tcatccagcc tttcgactgt ttgcccggtt ccatgccctg gcgatccgat 2820 ccccggtgac ctggtgcggc tgtcccatca tgcggagtga ttcaagggtt aggcgttgac 2880 gcttgcggcg taaactcaaa agcgataacg tgttgcccat atgggaccgg gaaacattca 2940 ttttctgccc ggcgttcagg gtaatgatct catgaatgga gatccggcgc tgtttgcttt 3000 cagcctcttt gaagatagcc agcggcggcg cgatctgctg gcgataccat tctaaaacca 3060 ttggcgataa agcgatcata tttcaccccc gataaagtcg gatcggtcgc tgtctctcat 3120 gcggtcgttt aattctttca gtgtggcgat cccgttttcg gcatctttcg ccaggcatga 3180 cccggcatag gttggatcac tccccaagcg caagcggtga aggtaacgca aggcggctaa 3240 cgtttggcgg atctcggatt ctgcgatcat ggttcggccc tcaattgtgg cggatgtcgt 3300 ggcgcttttc ggcgacggct aaaaagtcgt gaagcgcttt aatgttggcg gtgtgggcgt 3360 cgtgttcggc ccggcccttt tgggcgtcca gtttggtgac ctggtaagcc tccagcaagg 3420 cccgatattg tggatcatct gccagcatat agcagacccc gaaacggcgt gttatgccgt 3480 agcggttgcg agtgttggcc cccattttat gcggtcctct caacaatgat cactgtttgc 3540 gggatctgcc cctcaatcac tagcagcgct ttcttttggg taatgctgat tttaacacgg 3600 gttaacgtgg ctgtaaaatc tttcatttca tcatgcgcca gctttttaca aaaccaggtt 3660 tgcggcttgc cgatctccag cgagcgcaga aaatcagttt tgcctaaagt gtttttgata 3720 atcattctcg tttgtcctat attggcccct ttcggggccg ggccgggtta cattgctttt 3780 tcaatgtcgt ttaagatgtc ttttgcgaga gtgtagccga tttgcaagcc ccttgcaatg 3840 tcctctccca aaactttccg gcccatgttg ccaaaggata agatccgcgc cacttctgat 3900 ttttccatct gccaggaggc aaaacattga ttgccgttcc acttgctaaa gtgtgcgccg 3960 cctaactcct caaccgcacc aataacggca acgtttacag aaatgacgtg tttgatgatt 4020 tccatttgac tgactccagg acattgggcc ggggaccatt ccccgcccta tgagagacat 4080 tatcggccct attgcgccgg gtgtcaaccc cggatcatga aaaactttat tttgtggtgc 4140 atacgataga gccaatcatg aaaggcaggc ccccgcccgt taacgtcaat gtaaggcgtt 4200 ctaagctctt tttgctgttc ggcggtgggt atgtactccc ggccctcttt aatcatccca 4260 aaagccgtta catagtacac atacgcccca cactcagtaa agatcatcct gtttgagcgg 4320 atgaatcgga ttgtgtaggc gtcaccctcc tggcggatct tacggcccgc cgcgccgccg 4380 ttgtaaagtt tgcggatgtg ataagggtca ttgatcgcct tgttcgggtc cgctttaaaa 4440 cgttgccggg cctcttctat gtgctttttc cggtggatcg cctcgctggc tattgcgttg 4500 cgccgggctt gcgcctgcgc tgctggcgtc aattcgtcgt agtatttcag atctgtaagg 4560 ttcatcattc taccccgatc aatccgtcat cgtcgatgaa tacatgtttt tcaccaaagg 4620 atcgggcggc gtcgctcagg tcatcgccaa ccatccccaa cccacgatcc cagaatccga 4680 cgccgtgccc ctggcgggta aaccaaaaat cgcggcccgc ttgcgtgtgg tcgtagtcat 4740 ccgatccggc aatggctaaa tttacctttg cgccgtgcca gtccaggaag cgggcgcaag 4800 cctgttcgcc ctcatctttc gccgtggcgc tcaactcata cccggcgagg ttgtcgatct 4860 cttcgccatc gttcccggtg gcggattctg caaaatacag cgcctcaaag taaccagcga 4920 taaaggcgcg gaggtgtaaa gcgttgaaag tagtcatttt taccagccca ttttgatgca 4980 aattgcggtt gtggatgcga tagctacgaa aacagatacc aggaagatta cgctcatttt 5040 gtttctctct ttgtttgttg tggtctgttg tgacccgatg aaaagagtat aacccgtaac 5100 gcttcccggc gtcaacttta tttttagcat ttagtgctga tcattgtttc cagttcggcc 5160 cggcgggcct tcgctgcatc gcgtagcgtt ttcatctctt taatggtggc ggcaatggtc 5220 cggcccacgg cgcaagctaa aagccacacg tgcggccctt tcaggtcgtg atccttgtaa 5280 agcccttgcg aggttacagt aagacccaag cgggaagcgg ccttgtgatc cggcatctcg 5340 aaaatctgtt tcacttcttt gaatctcagg tgtttgaagt taagaccacg gatcacggcg 5400 gtagcggtgc ggacaattgc atccagttcg tttttgctgc actcaaccag gctgatttta 5460 tacattggtt cccacggggt gtacttgctc attttacgat ctctcttctg tttgtgggcg 5520 gcgttgtgtc gccctatgtg atgaattata ggccgggacg gttcccggcg tcaacaaaag 5580 ttttgtaaag attaaacgcc taaagtgtag gacgtgaaaa agatcacgcc gggcgcggct 5640 ggcttttgtt ctgccagctt ccaggctggc aacgtgtgcg ccgcgacata ctcccctaac 5700 atctgccagg cttgcgcctc atcgctggca aggatcacaa cctcttcaca ctccccccaa 5760 cggggggcgg tgtccggcag gcgctcaaag gtaaacgcga tcattttgca ccaaccatac 5820 agcatacaga atcaaaatga tttcgggcgc ggtgttcacc gtagcggaca cggctggaac 5880 ctgtttttgt gtagcggccc atttcgatga tttcgtaaca gtcaccgcca ttgtgaacca 5940 atgaaatgat aaacagttct gagactttgc gagcgatcac gccttgcgcc tccagcggtg 6000 gcaacttgca agccggggcg gtgcgggtga aaagtgattt gatgaatttt aacattttgt 6060 gaatcctatt tcgtttggtg tgggggtatt atggggccta tcggccccgc cgtcaactac 6120 tttttatttt atttcttccg gccctttcca cttcgaatag gtcaaaaatg cctcatgtag 6180 cgcaggggtc gcttcagaga gaaggatcgc gccgttatcg cttacagcgg tgacctggta 6240 atgatcggcc cggcgggatg attcaccgca agcggacagg gtaacagtaa catcaatcag 6300 gcgggacttt tgcagaatca ttgtaatcat ttttacggtc tctctctttt gttggtgtgg 6360 gtcattatcg gccctttcgg gccggatgtc aatcatttaa attcgtacat tacaacattg 6420 ttttcgtcga tgatttgggc tttatagtaa ccgccttgcg ccaggcgata agcggcgcat 6480 aggtcttcaa aagtgcggcg gatcggggag cttgtttcgt gttcccaagt catcaaagta 6540 aacatattca taacctctta gataatgcgg ggaccattcc cgccctatgt gagagattat 6600 aagccgggac ggttcccggc gtcaagctaa tttttaccaa atcgcaaacg ctttttagcc 6660 agggcgcggg cggttgtctt attgtgcccg gcctcctgat agtagcgcgt taagcggtcc 6720 aaagtttcac ggcgatccgc cttgctgggg taactggtga ttaagagttt atgcggattc 6780 atacagcgcc cccgctggga tcgtgtcgtt tacgatttcg gcgatcactt gctggataaa 6840 atcggcgggg atcggttggc ttttggcttg cgtgatccgg ttgccattat agatagcgtc 6900 ataagagatc acaaattctt tcccgtcgcg gtctacgttg atttgtaagc ggttcggcgc 6960 tttgtcatcc atatggcgac aaatgatctg gacggtgccc gctacgttgg acagcgacgc 7020 cacgacgtga tccgctacgt ttgccagaat gaaaagagtg ttagcctgtt gcatgtttaa 7080 agcctcgttt gtttgtgtgg ggtcattgta agccgggacg gtgcccggcg tcaacaatta 7140 ttttgcattc tttgccgggt gtggttcccg aaagtagcgg cggatctgcg ccgctgttag 7200 tcgggcgtca gcctgcaatg actttagcgg ataggggcgg ttagcaataa atgtatgttg 7260 cccgcaacca ttgccgatca cgatgtcaac cagggcgaaa agtaaagatg atttcatttt 7320 gcaccgcctt ttaacaattc agagatccgg cggtttgctt cctcttctga tacctcaacc 7380 gcgccgatca cggtcccatc ggtgagatct gaaggggtaa agccgtcacg gtgcaaccag 7440 ccaccacgcg ccccactcca gcggtaaacg ttgccatttt ctaagcggta aacattgcga 7500 gtaacaaaat aacgtgtcat gatttgcggc ctcatttgcc tgtttggtat gtctgagatt 7560 ataagccggg acggttcccg gcgtcaagcg cttttaatat tctttttgcg ccgggtttac 7620 gttcaggtga ttagccgccg ggataggttc taacagcggt tcaccttccg gcattgcttc 7680 aacttccggc cctgggatac aagccggatt aatccagcgc gggtcctggc ggtagccatt 7740 ggcgacgatc tcgcaagtta actgatcgtc gtatgcttca acggtgtaat catcacactg 7800 ctttaaagcg gcagcggcat aaaaacacat tccggcgaat acttcaaaca taattttaac 7860 cctcgtttgg ttgtctggtg ttgctttcgt ttgtctatgc cgtcattgta ggcgacggca 7920 taaccacacg tcaagcgttt tgcaaataaa attttgtgcc acgtgggagg cgtgatccga 7980 aaccttcccg atcacgtagg cgctttcttg cgtcctgctc atcatagcca taaactttca 8040 acgggccagc cacgccaggg atgtttacaa gatagtgttt cattggtcta actccggttt 8100 gtttgtctgg gggtattatg gggcctaacc cggccccggt caacaattat ttttgataat 8160 ttttcattct ttcggcgttg tgttcggcgg cttttaccca tgcgccgggg attgtgtcgg 8220 cccggcttac aaattcccca caaaaacggg taacatacaa ggccccgcaa gggtcgccgg 8280 aaaattcacg ggtcacggtg tagcgcttat cttttttgat tgtgcggata acttccccga 8340 aaagctgatt aacaagcgct ttccattgtg tgcgggcgtc gccgatctga tcttcaacaa 8400 cccactcgat ccggcccacg tcatcaaaat cgtgtgcttt gacgtgaaaa gcgccgtgtt 8460 ccattaaaag ctgtacccgg atatttttcc cggtgatctt tgtgtcgatc tcctggatat 8520 agccgcaagc cagtgcgtaa acggataaac ctgatttgat acggaaaatt ggggaagtca 8580 taacggtctc tcttctgtta gtgcggcgcg gtgtgcgtcg ctatgggtta gattatgggg 8640 gaaccggggc cggacgtcaa gccccaaaga tgaaaatatt ttaaattatt ttacagtgac 8700 ccggatcgcc tccaccgttg ccagtgtcgc cgggtcaact accagggctt gacccgtgga 8760 gatcttgacc ccttcgcggg ccgcaacggc ggcaagcgct ttgcaggtgg cgcggtatcc 8820 tttgccatcg gctaccaggt aaagggcgct aaaccccatc ggacgggacc ccatagcccg 8880 gataaaatct gttaaggtta aaaggttttg cattgtgtac cccatagata aaaaataagg 8940 ggcctaagcg gcccctgtgc ggtttaaact gtttggcctg gtgtttccat gccggggcgc 9000 ttactcccag ttatagcgcg ttttacggcg ctgttgtttg ttggccttct cacgttgcag 9060 gcgctcacct gcttcagcgt atgagagacg ggcgcggcgc ggttgtttat cgcggttaat 9120 gttagtgtga atcattctca tcttctccgg cgtttgtttt gctgctgttc catgtagtaa 9180 cgttgatcaa ttatttctcc gatcttctct tctgaggagt cgtaaacctc ataagtgatc 9240 accgcgccta actcctggcg gtagttcata gcgccaacca tatcgatctc gcacattgcg 9300 ccatagtccc ggcttgatcc cttctcccct ttgtactcat attcaaaata accgtacatt 9360 ttttgaccct ttcgccatag tgcggccttg tgagtggcga cgcagtaaag cgccgccagt 9420 gctaaacaaa tcagagttac gattgtcatt atacaccgtc aagcatggga ttcaataaca 9480 gagtatacaa caacccccag atatttagca agggcctcga aaaaatcgcg gggcgtatgg 9540 tatccgttac ccttgatccc catcttgcgg atcgcctcct gaaatgcggc atcctcgcga 9600 ttatagccat agccgccagc cttgccaaac tcagaaccat acagacccag gatcggggag 9660 tgaatccaaa gaatcgccgt gttagttttc ccggtgccgt agaagcggac ctccgccaga 9720 tctttgatct cattgtctac cagcccgacc aggtgaacgg ccttgtaaaa attgttttgc 9780 ttacgggcct tgcccaggtc gccagaagtt acaaccatgt tggaaaaatc gtaagtagcg 9840 ttcataatgt tttctctctt agtttggttt gtggtgtttc tcttaatcag tcccgctatt 9900 atgccagcgg gacggggtaa gagtcaacaa ctattttaaa ctttttgcca gcttatcgat 9960 cccggcgctt aatttggtgt gctttgttgc ttccagtaat acggaaacct gcattaaaat 10020 attttccgcc tgttcagggt acaactcaat aaccagattg aaggcggcgc gggaaccact 10080 acggatcgcc acctggtaag cctcatgttt aacgtcgttt acttgctgtt cgttgatcat 10140 gctgtcaatc tctttctgtt ggtgtacggg tattatgggg cctaacccgg ccccgtgtca 10200 acataaatta taaatctttt ttgcggctaa catcataaac gctttcggtg tagacaaccc 10260 cgagatccgc cgccgcctgg caagcgtagg acataccacg ccacgccgcc ggggcatcct 10320 tcaccgcctc gatcatcgtg cttaatgcgg tttgttcgaa cccgcgatca taaccatagg 10380 cgaaaccaac ataagccagg tcgatccaca cgtcgccgct tttgttgccg tacttgttag 10440 cgcctaagcg gtccgccact tcaacggata ccagcgcccc caggcttacg ctgtgataag 10500 tgttgccgta gctacgctgg aaccaacgtt tgccagttac ggaaatttct ttgatgtcag 10560 cggcgtttac agtgttgata atgttcattg tgtgatcctt tcggtttggt tgtttgcttt 10620 cgcttgtcta tgccgggtat tatggacccg gcagaaacca acgtcaagcg gtttttacta 10680 aatcatcaaa gaaactttgc ggacgggtgg cgcaaccttt gcccgcccat ttgttaacgt 10740 gctttgaggt tgtcgcgctg tgctttttgt cagtcacgac atagcccacg ccaggcaggc 10800 aagccgcaac cggggtattg taggaaaaga aaacttgcgc cccggtggag gtagtgatca 10860 tggtttggtt agcgccgata gtagtcagat tcattttgta agcctcgttt gcttgtctgt 10920 ttgatatgtc tggtattatg gggcctaacc cggccccggt caacaattat ttattacaga 10980 accgtaattt tttcgccttt gatcattatg taagtgtgcc cggcgttgct gtactggtca 11040 acatagatcc ggcggcgctt gccttgataa tccaacatat aggacgtggg gatcttggtg 11100 ccatagcccg tggcggtttt ttgcaggccc tggcgttgcc attcaagcgg ggcgtagatc 11160 ggtgcgtaga aatctgaagc gtgatccggg tggctaatca ttacggtttg catactgtta 11220 ccttttggtt ttttggcggc gggttattcc gtcgccctat gtgatacatt atggacccat 11280 cctggccggg ggtcaatacc caaaagcaaa ataatttaaa tatttattcc gttgtctttt 11340 ggttcgacga tcccgccatt gtccaagatg caacggatca cggcgtcgcc catcgcttta 11400 catagcgccc acgtcggcgc ggtgatctca atataaggaa agcaagcggc cccgccgggg 11460 aagtgttcca cgtccgccag gttatcagga tcggcattgt gtgcggcgtt gcttactcgc 11520 ctgtttaatt cgtcccattc cgggaaatca tattgatcat cggtatcgcc gatccattgt 11580 cccgcaagat cattacaatc ataaaagata tggcaacgga aaagcggcgt gatctggaca 11640 gcggggatct gtttggtcat ggtactgact ccacacgaaa ggcccccgcc gggggcctgc 11700 aattggttag cggtgagtgt tgcgggaaag tgtgccgccg ttatgctttg cggtaactcg 11760 ccagccgtcc gccgttttgg ttggcgttgt cggggtcacg ccagcggcgc ggatcttact 11820 ggcaacggcg cgggcctcgt cacgggtacg gtatgcgggc agggttacaa ccgccgccag 11880 gttaacagag gaaacgccac gtgatttttt ataggttact ttagccattt gatttactct 11940 ctcattttgc cgggcaccat ttgccgggct tgtcgtgaat tatggggcca ggtggccccg 12000 atgtcaatag tctttttaaa ttattttgcg tagcgctcgc cattcataaa ggcgatccag 12060 cgggccgctt gccctggctt tttgaattgt gcgatcaatt cgtcgccacc gtgacgggct 12120 accgcctcaa cataatcggc gctgtttggc gcacattgta ccaggttacc cgtgttaaaa 12180 tttgggtgtt tataaagtgt gcggcggctg ttaatactat caatacggat gattgaagag 12240 tccaaaagcg ccccgccgcc atagcttcgg ctattcttaa ccagcagggg gatcttttgc 12300 tcgcccatgc tgcggcctat cttcccagta acatcatatt catctaacca tgattcgccc 12360 gtttcggtgt tgccgtacca tacacggaca cgatcgccac ggttataagc acccatgata 12420 gctttttgaa ctgcttcgga agttttagca ttgaatgtaa acattttacg gactctcttt 12480 tgtttatgcg gcgcggtgtg cgtcgctatg tggtgaatta tggggcctat cgctggcccc 12540 gtcaagcatt attttaaatc tttttgtaat ttaattttgt gctttcctgg tacggcttgc 12600 ccgttgtcat atcatagatc acatattgcc cggcgcggtc cgcctgttgc agtgtgacgg 12660 cgcgatcctg gcattgcttg acgccaccaa tataaaccgg gtccaggtcc gccagcttaa 12720 cagcgccggg accaggcagg atcttaaaca cggcgaaacc acgatccaca ctatcgaacc 12780 cggcgaacca ttcgcggcgg ttctcttctt tattgtagcg gcaggcgcgg atctgcccgg 12840 cgtgggcggc ggcgttacct tcaataaaat atttcgtctg ttgcatcggt cgatctcctt 12900 tgttggtgtg cctgcattgt ggcccggctt gcctggcagg tcaagaaaaa tttattttcg 12960 ttttggtatt gacggcccgg cggggctagt tcataatgta cacatacagg agaggagacg 13020 ccaccccacg gcgaaaaggc ggggctaacg taccgggcac cggggccagg tcggaaggtg 13080 gatcgccggt agttcgtcca aaatagtttt gtaaagaact gtaaagacgc ttgcaaccgg 13140 gccggggttg ggttatatta tacacatagc ggggagaaat gacccggaga caatcaccgg 13200 gggtgcccgc tattctggag atgagaatga ttctcattca actacgtaaa tgcgaataat 13260 tatcatttca atttgcaaat gagaacgatt ctcatttcgc ctcgggccta acgtgaatga 13320 taaaaattct catttcgatt ttcacctctg tatagatttt gagaatacgt ttttgaaaat 13380 gaaatctcga tcgagacccc gggcggagac ctgtacctgt atcgagaata cgatctcgaa 13440 attgagtttt cgatcgagac cctgctttct gtaccgagac cccagtccgg cccggcgggc 13500 agagaatacg atcggcaaaa cgaaaaaccg atcgagaccc tcaaattttt tgaccagaga 13560 taactcccgt attgtaacgt atatgtgccg ttagtggtgc ataagcccct taatgtaaca 13620 tttatggtac attagccctt gaccggggcc gggtgtgctg gtacattgtt gagtaaatct 13680 gaaaaaggaa tctgatctga gtcccggccc ggcgggccat ctcaaataaa atctcaaatt 13740 gaatttcgaa ttgaaatctg atttcaaata tcggcgcaaa tatcacaggc aaatatcaaa 13800 tcgaaatccg atctgaaaac tgagccgtcc ttggcccaca aatatcacag cttggtgcta 13860 accgacacct gcaatgtgat cggtttcacg ttcgagatgt agttcttccc ttcgtaatcc 13920 ctcaagtatc tgaacacaga ccagttggcc ccagggttga ggacatcaaa cagggcaatc 13980 tcttcttccg ttggtcgctt ccagtcatca aagaaagtcc tgaactcttc caccttcatg 14040 gcgagacttt ccttgccatt ccccaagacg atcttattat cactggtgaa gtacaggtag 14100 cctggcaccc actgggtgga taatcggagg atgccgttcg aacacgcctt gatggtcaag 14160 tctccaagtt tggaatcttc cagatccccc atgtacgtat catcgtgggt atatgacaac 14220 caggcttgac ccagtgcatg gtaatcctga acaaaccggg ctacattgta cccgtaccaa 14280 cccataacca catctttcat tatgtgggat tgtgggaggt actcttcaag ttggtccttc 14340 aagttttcga caaaggtcac catctggtca cgggtcattc catccgggtc ccagttcatc 14400 ttcagatcgt aacacgaatc acgcacagcg cggtgcaaag tatctatctt ttcgttggcc 14460 ctgtaaacaa aatcctcaag agaagaatca aagtcaaaat cactggctat gtctttgata 14520 tccgaagcta cagaatcatt gctctgccat tccttccgca aagtatcttg gacatcagac 14580 agccgccgct gtaaattttt ccaatcgggg atctctggcg ttggactggt gcccttggaa 14640 taatcagcga caccgtgtag aaaagctgca tcaaatgagg acatgctcta aaagctcctg 14700 taacgcaaca ggagaggcgc aagcctctcc cggtatgatt tatcaaagac cctttacgag 14760 gcccatgtgc cactcaggag agcctctgtg gggcacttcc ttcttattgt cttcgacgaa 14820 gacctctgcg cttgcaacaa cacagccgca ctcgatggct ttggtcattg tatcccgcat 14880 tgcattagga caaacaccgc tggcaatggc ttgcttcgtc agttgcccgg caatggcccg 14940 cagcttgtcc caagcctctt gttggtaagc ctggaactca ggtgtcatct taccgtattt 15000 gtcgtagcag atcatacgat accctcgtgc attttcatac ccatgacttg ggcctccaag 15060 ccccggctgc ggaagtagat aaccgccttg cggaacccgt cttcattgtc aacctgattt 15120 acatatacgt aatcaaggac aacttcccct gcatgaccga acagcttatg ccaccaacga 15180 acccggcgga tagccgtgct gttgtacatg atgttggaca agaaaacgta ctttgtcatg 15240 atacgaagtc cagtgtggtg cccagttctt ccatacgctt cagaagccac tggcacactt 15300 tgtggaactc ttcttcatcc tcgatgtcgg cagtcaggta atactggcag aactcggcat 15360 cgtcggtgtg aagcgcgaca gacccgcaat ccaagtcgat gaactcattc aggatcgtgg 15420 tttcaatttc gtcataacgg tcttcgtcat gggggacgaa gaacattacc ccggtgctga 15480 tagtgcgtgt gatcttcagg tctttcatgt catttctcca cgtaatgagt aaggaaagtt 15540 tctaccgcca atgcggcgtt ggtttgataa aagaaatcgt tcaccgtccc gtggaacata 15600 ttagccacgt caggaaaatc ttcgttgacg tagttccaca ggttctggcc tactcgtaac 15660 tgagggtgtg cccggttaag agcataggcc ctgtcaatga gtttacacgc attcttctgg 15720 ctaatcaact tcattcgtgt gcctccgcag tgatacggtt tacccatgac tccagaccca 15780 ggtttaccca gttggagaac tcttggccct cttggatgcg ctccagtgct tcaacggcac 15840 cgagggcctt gatggccttg atgaagcgaa ccatctgcgc cgggttgtcg agactcagtt 15900 cccgcaccgt ttgggtgaag tggtggtcgt tatcatccat gcgggggatg aacgcatcca 15960 gttcccagcg ctccgcacca ccggaacgca tcgccatgta ctggcgctga tctttctcgc 16020 agatctctgc ttgggcttcg atgttgcgga tattgtcgaa aatgtggctc atttgttctt 16080 ctccagttcg gcaatcacgg cggacgcctg ttttgtattt cggaagttga ttgtagcact 16140 ctgtcccatc atgtcaagac gttccagcag agtttctgcc gcatcatcct cacccattgc 16200 ccagaaaccc ggaaggccga ggaactcaca ccagtacgcc ttgcctccgt cgtcgatatc 16260 cgccgggcaa gtatcgtcgg tgtatgtcca atgacccaca ccgaacccgg acaggccgtt 16320 acgggtcagg atcagttccg gcatacagtc acaggaatcg ttgatgtggc actcgcagtc 16380 accgagaccc tggcggacca taaactgcgg gacgatttta cgaatatcca ttacagatcc 16440 tccatctcga acagtgcgtt gtcatactcg ccgccgatct tgtcttcgta gaacccgatg 16500 ttggcattga tgattgccag tgccttgttc acttcctcgc ccttcacacc tgcggcgcgg 16560 agttccagtt cgaaagcctg ctgcaagcga cgaactgcct cgatgtggcc tttctgtact 16620 gcgacacgag ccaggtcatt tgcttttttc ttcatgctca tcttgtcgtt ccttttctct 16680 gtttcgtttc gatggactaa gaatagcaaa aggggttgac cgaagtcaac ccctttgatt 16740 aaataaattc aaattttacg aacatcccca gcaaagcggc gtttttgtcg tcgagacgct 16800 ccgcatacca ccgctcttta tcctccccaa caacgatcat ggagttagga tctgccgctg 16860 actcgtcaac cttgcaggca tattctttac ctacggtgaa gtggcattcc atatcctctt 16920 cgacatacgt acagcgtacc ttattcattg tttcctccgg gttttataac ttcacgaacg 16980 agatcaagaa tacgctccag gtatgcgtat tggtggcttg gcattttagg ccagtcaatg 17040 tgccacggct cacacatccc ttcgatgacc cgcaactcct ccggcacatg accgtaacac 17100 aaagagttac ggtccgggct gtagctatca acgtagtacc acgcctgttt ggcttggtct 17160 ttggtgatgt ctccggaacg gcggtgtttc agaattaaag acttcaggta gtccgggtct 17220 ctgtccgggt ccggttcagt ggctcggtaa tcagacatct tgtcgatcag gtagtcattg 17280 gacacacgct ggaagaactc ctccactgtg ccgcccatgc tgccccagaa gcctgtccag 17340 acctgcccgt agcaagagat ggtaatcttg ccagtgcccg gttcaaagtt ttccacaata 17400 acctcaaccg ggtcgagacg gtgggaatcc tgaatgtctt cgatacggat cttttcgatt 17460 gtggaacgtt taatctgcac ggcgggcctc cagttcacgg gcaatttccc cgccctctgt 17520 ggtgagtacc cagccaaacg gcgggagata acggatcagt ccttgggaag ccaggttgtc 17580 ctgaaccttt cccttcaggt cacgattgtg gtcaccaccg agtgcagcca gcagaaattt 17640 ttgctggggt ttagttagtt tagccattat ttattccttt atcaataaga ttgtgctgtt 17700 atctgccgag aatatacact tccccggtgc gggagatggt atattgaggg taagttttac 17760 cctcttcggt tgtatatgtc cctttctcca ccagaccctt tttgacaagg gtctcaagga 17820 tattgatggt tccctggcgt gtgttccaca cccacccaca gccccagggg aaggcgatat 17880 agccgtaacg accttcgctg agacacttca ggaccccgat ttggttttcc caaggttcat 17940 acccgttcct tgtagaaagc gtaggcaaag tatgctttat tgcgactatt tttcacgccg 18000 taataggtat catgcccgta accgaacaac cacccggtaa agacaagggt atgctcccag 18060 aaccgccact tatacttcag gtacgttttc ttcacgtgtc ttctccttac gtttattgtg 18120 caggtgctca acaacacgga ccagcggccc ccggcggcgc ttacggattt ggcgttggat 18180 cttccacgtc acgtaacgag gggactctct gaacttatcg atcttcctgc cgacccagaa 18240 catcaactct acgaacagca gcacgggtgt catcaaaaag caagccgggg taagcataaa 18300 aaggagcatt acccggtcat cattacctac acgaccagaa accgacatca gggacaacat 18360 acgccagatg ccgaccactg agaacagtgc ccagaggaga tagaagtaaa tgtgctccat 18420 catgcccccg gctgaatgtt ggaaggccac cagaaatact gctgtgccgg ggcacgttta 18480 ccatcgccac ccttatcttc gttcgccgca ggcataagtg cccaccattt gatattaccc 18540 attataacct ccacagattt ggataacaaa cacaatgatc tttccgatca cgcctaacag 18600 tagcatagac ccaccgagga ttacaagacc cttatagatc ttatccccaa gttccatacg 18660 gattccttcg atattttgaa tagaagaaat ccacaacaag tgtcaaagca ataccagcaa 18720 tcagcacggt catttgacct cctcatcaca atatccaaac acacccagac ctgcaaccgt 18780 gatcatatag gtcttccaac cgatacgatc atcaacacct tggtagatca ggctgatcga 18840 acctggtcca aacttcaccg ggaagtccat caccatattg atatgatctg ccagagcctc 18900 tttggtggcg gggatctgtt tcacagtctt caggctttcc accagtgaac cgcgatgctg 18960 tcgaactctc atatgtctct ccttcatgtt gtttgttgtg tgtgggagtt ctctcccttg 19020 aacacaaaga tacatgagag aaatgttggt gtcaatacaa tttcgaagaa atttaataaa 19080 actgactccg aacgaatgtg agtggggaag ttttattata agatcagatc aacaccccgt 19140 aggcccggat gggtgagcgt agcgaaggtg ttagatctat tattacttcg tgtgcctttc 19200 agggctacat actatgttgt cttttctgcc cactttttca gagtcgggcg aatagcatca 19260 tgcaacatct cttcgttatg tcccgctgcc catttggcaa ccaagaaaga gcacagcata 19320 atgaaatcat gatcctcttt taccggagac atcgacagaa gatccacaaa ggcaagttca 19380 tcctcgtcca cacgatccgg gtaaaggaat gtctcaaaat gggcaagttc tgtctcgata 19440 ccctcggcat tggccctgac aacttccata cctcggctgt tgaacatcga tcgccaagtg 19500 tattctacct gcccagcctt atccaaacgg aacacagaga gggtcgcccc ccggcgacca 19560 cactctaatt gataaccata ctgtgacata attctcctta cagatatttg tcgaagtcga 19620 tggcagtgcc gaacggaggg gtccattccg gtccgtgacg gcccaggatg atccacaacg 19680 tcttcttgcc atacttctcg gcccagtttt taacattgga cacatcagag aagtaaccat 19740 ctgtatagat tacaatctga tcgggttctt tgtcaagatt atcaagacat tcgaacacgc 19800 actgcacatc ggtcccgtag gtagtcttga tcttgtagtt atcaatctcc ttcaggttct 19860 tcttcgtgta agtacagata ttaccaacct ttgtggacca tgccgccata gtgacctcaa 19920 agtcctcgta ctggttgcac agacccttga tctcacgttt ggtcggggcc agcaaatcat 19980 cagtaaaaga tccagacacg tcgaagaata cgtgagcacg gattgtctta gccttagtgt 20040 acgaaggaag gccgatcgtc tgccgggaag taagatgccc tgcttgacgt aaagatcgcg 20100 tcagggagta gctacggcgg tttaaacgac ggtatgaaac gttgtctttg aacagccgct 20160 caattgtccg acgtaacagt cgcaggtagt taaccttcgg ctttcctgcc tcctccacaa 20220 acttacgagc atccgctggc gctcccttgc ccgctgcctg aacagcgttc tcgattgccc 20280 ggcgcattac cgtgtcatta tagttcttct gctcctgggt cagcggcttc atcgcccccg 20340 ggtcagtacc cattgctttg gataagtcat cctcgccatc ttgctcactg aaagtgccgt 20400 cagcattggg gtgctggtca gtacccatcg gcccattgcc ctgctgcgat tggtcacctt 20460 tttggtcacc cttctcacca tctcccgggg cgctgccgcc tccaccaggt ggtgggggtg 20520 gagtattttt aaccaagagt tcgtaaatct gctccgcgta catgtcggca tactggaaat 20580 cacaatacgc ccacttcatg aaaccaaact ctttgtccgg atcgaacact gtcttggcac 20640 ccattgggaa ccaagccgta cccatctcac tgtttgtcgt atgattcagg acagcgttgg 20700 tgttgatacg gtggtcggca gcaatgttga acagggcgaa attgtaaccc tttccacggt 20760 acatgtgctc agaaaccacg tgatcgcact cgtgctccaa caccaaggcc acctcacggg 20820 cagtcttgcg acgatagaac acatcgatca tcaacttcat ctcagcattc ttcttcttgt 20880 cggtgatttg ctggtcaatg cgctgtttta ccagcgcctt gcgcttatcg cacatcccgg 20940 caacaaattc cggagaatag aacaggtcgc gcccattggt agccatcgtg ttgatggtct 21000 ctgtggcacg gataatcggc attgacgcca acataacacc atagaaaggt ttgtagcgca 21060 tcagttgaag acttgcagcc tcaaggagtt tctctgcctc ttctgtattg gcgttggcaa 21120 gctcaagctc ctcggcactc atgatattgt cgaacatatt tcctccaaaa gaaaaagggg 21180 ccatctcgaa agatgacccc attctatcag cttatattgt gatgtcaacc tttaacgttg 21240 aaatcctcca tgattttacc ataacgagtc tgatactcca ttacgatagt cataacagct 21300 tcgcagttct tacgcagcgc cgggatcatc ttggtacgtt tcgggttcag gctcaggatc 21360 gcctgttgat ccgtattgga tgcacggaag ataccaatca tcagcgcgat catggtgtct 21420 ttgttcgcct tgtaatagtt gttgaacatg aagttcagga agttagaagc acgttcgatg 21480 aagatctcat cattcatgcc attgtccatc acttctttct ccagttgacg taagcaggtc 21540 tgtgccagta cgtaaaccag gtcgttcttc tcacgagaca gttcgtgctt tttcaccttg 21600 ccggagaaga tgtcttcaac tttcggcagt tcagcggcac gtaagtggta cgcaagaacc 21660 gcagtttcca gaccttcacc aacagaaccc gcgatagcga tctcggtgat gtactcattc 21720 ttctgtactt cgtaatcgta cagaatgtca gaaacagttt tccactgacg aggtgatgcg 21780 aacaccggag tagagcgctt ctctacgtca ccagtgtaga aatgctccgg gaactgcttc 21840 aggaagccga ttacgtgtgg gtgcagtttg aatgggactg cgaagtcctc aatccacaca 21900 tccagattgt attcaacctc tgccatgatc atacggttaa ccagcggccc cggcatttcg 21960 gtggtgcctg cgttatcggt gtcacggtta cctgcaccaa cgaacacaca acctttcggg 22020 aagcggtggt cacctgcaac gtgatccaga ataacctgat acgcagagtg ctgagtgccc 22080 agcattgcgt tcggcagttc atccaggaat acgatacctt cccagttcgg atctgtaggc 22140 cagaagctcg gacatgcgta cacagtacgg gtaacttctt tgccctgaac cggatcgaaa 22200 taggttaccg ggaccgggat acctgcaata tcctgcggct ctttgtcaga caggcgaacg 22260 tcacacaggt tgtccccgat gcggtggggg aacatgttgt tggctacctg caacactttt 22320 tgggacttac cgagacctgc accgccgaag aagaaggtgg ttttaccgat acgggcacca 22380 aggcggatca gatcttccat ctgtcggata ctgattcgtt tcttaaacat ggtttggtca 22440 gtgcgtttgt aagtctgctg cattgaattt ctcctctttg ttgtcaatga ggcccattat 22500 atatgggcca tctgataagt caacactttt atccaccaaa agaactgcta ctgcgcccca 22560 cagaagaacc gaacccgcca cgggtggttg tctttgacga ggagaagctg gatttggaac 22620 cggaccaagt ggatcgcggt gcctcaacct ttggcttaat ggcagacttc gttactttga 22680 acgatttccc cggcttcacg tcgtaagacc gggccttgtt gtcaacccac tgaccagagc 22740 ggctctggta catcggtgct gcgtgatagg agttacttga ccccatcata ttacccagca 22800 tgtaacccat catcaggggc atccacgggg attctccgcc cgtactggca gcagccgccg 22860 agttatgttg gcacatactg ccaaactctt cgtaacactc ggcatacgtc ccaaatttcg 22920 gtgccgtttc tgcggcaacc ttctgggcct ctttctccgc agtttggcac tgttcgagcg 22980 tatttccgct ttctttggca cactgctcag agttcatgta cagtttcacc gtttcgtcag 23040 cttgctcaca gccggacagg gtgaaggtta caacagccgc gacagcaaac cccagaaagg 23100 atttacggaa tcgcgccttg ttgattactt tggtacgctt cattctcaac cccatgcttt 23160 caggccgtaa cgcccggtaa agaagatttc gttatcctgg aagatgccat ccgggttctt 23220 gcggaacagg tccatgtagt aatcacggac cgcatcccac tccttcttat cgaagtacac 23280 cacggcctcc ccagtgccca ccacgccgtt tttataaccg tcggatacct tacccatgac 23340 ccggacggtg gcctttttac ggtggatacg gacgttctcg tgatacccgg cgcaaccgcc 23400 gtgaccgcag gaacaggtaa agaacgtgtt ccacgtccaa ccgtcctgat aatcgccctt 23460 atagccgggc ttcattgcct ggaggaagta gtacatgcag tacgtctcct ccggctccac 23520 cttgttgatt cggcaaccga tacgtgtaat caggtgtttg gaatcctctt cgagttccaa 23580 ccaaccacca tcatccatca cctcggtact gccgtaacga tccggtgtcc ccatgtagga 23640 ggaaatgact ggctcaatgc tcagggtctt tttcattctg cctccaccag gacttcaatg 23700 aagtcgtcag cgccggacat ggcgcggtag taatagtggt tcacttcacg gacgaaatat 23760 ttgttgccct gtgcgtggat cacttcgttc ttgcggggaa cgataatagg cccgttgtcg 23820 gagctacgtg tcgccaggat tacgtccaca ccacgttcaa tgatttcaag tttcaattgt 23880 cacttcctcc cattccatat cgctcaaaaa gcaagattcg caatcgtcat caaaaacgaa 23940 ggcatatccg tgagtcccgt ggactaaata atgtttgtcc gggtcgaatt tatgccccaa 24000 gggggcatct aaaactttaa tccgcatcgt attccttctc catgattgat ttaacctgct 24060 catcacgggc agaaaaataa tcgcccggga aagagaacat gctcaaatgg ttgtcttcgt 24120 accatgcgat catgtcttta atctgcatgt gcattttatg atacttctgg attcgctgca 24180 agtctttttt acgcagtcct ttcagacggc ggatatcggt gttgtgacgc aggtctgcca 24240 gcttaacacg gcaggcgtca taactctcca ggataccctt gagatattcg tcgggggtct 24300 gaccttctac cttggtgagt cgagcgatcc cggagataac cctgttggac atacccaacc 24360 gttctaaatc acagggttgt accggggtat cttcaacgat gtcgtgaccc acggcaatac 24420 agttcagctc gtcgtcatcc tttactttaa ggtagtgcat taccttcagg acgtgcagaa 24480 cataaggacg cccgccctta tcaaactgtc gcatgtggtg tgtcgcagcc aacttaatca 24540 tcatggcaag ctgctctgcc ggggataata ctttgttcat ggtagtctcc tgttcatgtt 24600 tagtgaacaa taaggtagca tgaacaaaaa tacgatgcaa gggttgattt cttgatcgag 24660 acccggtata ttgccttcat caaacacaaa cgaggaacga aaatatgttc gaacgcacca 24720 acaaccgtga cgaagagcac gtcgatgaac gagcctacaa attcttctct tatatgtacg 24780 gaagagaggg tcatcattac ggctggaaca gcactaagga agggattcgt ccgacgaccg 24840 tgctgtctga caaatctacc cacgtctgtg cttactgtgg gcgtatggcc ctacctatcc 24900 aaggtgagcg ttgggaccac ttcaaggata gccgggagta tttctccaaa ggtcattgct 24960 gcgtgtgtaa agaggcaatg gacgaactgg aaatgatgga tcagttgaag attatcgagg 25020 accagtttga aaatgcccgc agcgctgtgt accgggcaat gccgaagaca aatcctgaca 25080 tcgttgcggc tttcgtggac cgaaaggcgg agcaggtgaa aaaagacttg aagttctgga 25140 acaataacgg cagtatatcc ccacattcac tggataagac gggccttgaa cttcgtggcc 25200 cacgttatgc gaaaggggac tatgatgaat aagctgaaga tgtggctgaa aagattcgca 25260 ggtattgaca ccacgcccga tgagttcaac gcctggttcc agcataagct gggccaaggg 25320 ttgcgtggcg gtgatgtgta ctttgcgata aagatggttg ggtctgacac ccttccggtc 25380 atcaacaaga agttccaagt tttctccaaa ctgaaaaatg cagaaaggaa aaaagaagcc 25440 ttgacaagag acaatccagg tattaacctt gagattgtgg ttgtgagaac cttttattcg 25500 tttccggagg catcatgaaa gaagacctga ctggacagat taacctgcgc ctgtcgaatg 25560 caccaaagac taacgaaccg gacaaacgcc tcattggctt tgtggtgggt gtggatgaac 25620 tggagcacgg gatgggctgg ttctccccgc tgctggagca atactgtgcc ctgtacctga 25680 aaactaaagg gtacgatgtg atcaagaaac ctgttaacga ccgcctaacc ttggaggaaa 25740 gagcatgaaa cgtacacgac tgattctgat ggctgcactg atgggcttcg tcctggctgg 25800 ttgcgacgcc agcacccgtg aatccactga cagctatgtc ctcccgaagg acctgctgga 25860 aaagggctgc aagatctacc acatggaagg gaagtatgag aactccctcg atgtggtgta 25920 ctgcccgaac gcacaggtga catcccgcac taaggcaggt aagtcaccgc agcggtctgt 25980 taccactatt gacggggtgt atcatgggtg agatcgtaga gactgggcca gacctcttgg 26040 aaaagttctt tggtcgtgaa ctgtacgggc atgaccgcca gagcatctcc ttgtacggaa 26100 tgccgaagta ttgctgtggg gacatcctgc ggcttgatgg gagttcaggt gccgggtacg 26160 ccgggcatca gttcgtagtt gaagggatgt tttggtcgga cttactgcac tcttgggtgt 26220 attacgtccc ggcttttaat ttttaccaat gtgaatccaa tcttgaggag attaaataat 26280 ggaagctgta atcgcgtttt tcaccgctga acctgctggc gcactgatta ccctactgac 26340 cttcggactg atcgggctat tatggctgtt ctgtgtggaa gtgtcttgca tctatgagcg 26400 cgtccgcgcc ctgttcaata agggcctgcg acaaggttac acgtggaaaa ctgtggcttg 26460 gatgccaatc cgaatctatg ttgtgatcct catcccgcag atcgtgtaca ttttgtttac 26520 cgaaatcatt aaattcggtt gacagtaata aatggggcct gtattatggc cccataccaa 26580 cacaaatgag gaaacaccga tgaacaatta caaatcccaa ggtcagactt tcgaagaagc 26640 tcttgttaac ctgtctgaag acggcatctt tgtcgaagac ccattcttca agcagcacat 26700 ggaaaacatc cgtaagcagc gtgagttggg cttgggattt gatatggaga aggcgaaaaa 26760 ggttgtcgaa gacgccgtgt ctggcatcga ctattctgac ctgccgaagg atatccgtaa 26820 tcgcactttg gttatcgaca ttgaccaggt ttgtgcggcg cacggggtcg agaccgccga 26880 gcagcgtgaa gctctcaagg acaagattgc caacatgccg gaagttcgtg acatcaactt 26940 ggtctgtttt gatgaggtca atgcaccgaa agaggaattt gtaaccttcg agatcctcaa 27000 gggccgtagc tctaccgggc gtttcgatta tccgttcctg ccggacatgc accacatgcc 27060 acgcctgaac aagacgtaca tgccacctaa agatggtgat gcctttatct gcatggagag 27120 tgagatggac tggtcccgcc tggaagaact ctgcaaggcc gaccttcctc gcccggacct 27180 gcacatctcc ggcgatgacc cggcactgaa gatccgtaag cacccgatga tcttcaaggt 27240 cactgacctg gccgaggaca aagaagagtt tcctcgcctg aagaagactt tggagcgtaa 27300 ccaacaggtg gaagccatgc tgggctggcc tgcgccgccg atgttcatcg aagccatccc 27360 agatgccaac gttgtggccc gtgcgatgga tgaagtgatg aaagagttga cagcgctggc 27420 acgagagtat aaagtgcaaa tctacttcgg caccaaacct gcattgaaga ctgccttgta 27480 ctctttgatg tatggtgcca agaaggaaga cctcgatatg tcccagcttg acgagatctc 27540 tgtggagaaa gccatcgaga tgtacgaaag caagctccgt aaggtagccg tcatcggcgg 27600 gcacgggctt gggtttggga agatggcaaa gatccttgct gatcattaca gcaccgagac 27660 tgtctgtctg gactctttcg acacgtggaa cttcaaagac ttcgccaaca ttgcgaagct 27720 ggacaagcca gcacgtgact gggaacaggg gcgactccgc cgtggcaagg gccacaacaa 27780 atttaagcgt aaggtgaaga aatgagaaag tatattgatg gtgatcgtga ggataaatac 27840 tcttcgacca cggcggtggt tgaaccacgg gggtatcgcc aaatagtttt ggctgtccgc 27900 cggactggcg ggccttgttg cctcggggag gagtttatgt acctttctcg ggagaaggcc 27960 cgtgaactgg cccaagtcct taacgacctg gcagatgaac tgtttttcga ggagcaacgt 28020 gcgaatggaa atgaatgatt ttgtaggaaa cccactcgca atcggtgatg aagtggtgtt 28080 tactgagtat aaccgtcacg ggatgtctcg tggcaagatt atcggcttct cgaaggcttt 28140 gaatatggcg agcattgagt acaccaccga tggtggctcc gttcgcaaga caaagaaaac 28200 ttcgggatat gtggtgaagg tggtgaaatg agcagagaag aaggcaaacc gtacccaaaa 28260 gagcgaccaa cgatccgttc tgaaatgacg ggttcaggtt gtgatcgtat tatccaagaa 28320 cacccggcgt atggtcgaat cacactgacc cacccgtcag gtggcaatac tgagatgttt 28380 ggttcagaca tcatccataa tgagcgcata gcgcttcgta ttgacctggc gtatgaagaa 28440 acggcctacg gtattccaaa ctaccgcagc cagccacaga gccgtggagg gcgtgtgctg 28500 gaactggaac tgacagctta ccagtgggca ggtttggttg catcccacag tgggaatggt 28560 gtaccgtgta ccttgcgtta cattactcct catgggcagg gcaaccttcc cctgattgat 28620 gggcagaaca ccagtaccga gcaggccaac cgtgagattg aggcatctct caagatcatg 28680 atgaaacgcc atgcggaggg ccttgaagcc ctgtcagcgt tgattgccaa aggcaaggcc 28740 aacaagggtg agttgcaggc ggcgatggag aagttccaga ccattcatgc caagctgcca 28800 gaagtgacgg cctatgcact ccataccttc actgagcact cagagacggt catggcccgg 28860 gcaaacgcag agatcgaagc gtccctgaac agcctgatcc agcgtaccgg gatcaaggct 28920 ctgggcatga agcctctgga gttggaggat aaagaatgac agattgggcg acagtagagg 28980 acctgctgac ccgtattgat gccaagatcc acgggatcgg taacgacgaa gaggacgtgg 29040 agttcgagct tcgtgaggtt atggatctgc gtggggcgtt cgagggagtt cgtcatgagc 29100 tttatcacgt tcgtaagtgt ggcatcccgg gctttacccg tggcgacttt gttcgtgaca 29160 agttcaacgg caagtcgttt tatgtgagcg atgccgaagt acaggaaggt agcgacgggt 29220 acgaatggtt ctatcgcagc gggttctact tcatcccgca atcagatctg gaactggtaa 29280 aaccgagggg tgttgtatga cagtaaaatc tgtgaagcag caaaattgtg agcataagtt 29340 cattaccaag ggcagcgtac acgcttttgt tcgtgaatgc acaaaatgcg gctggtacga 29400 agtctgcgtg gggtaatcgg atgcaagtgt tggcacaaga catcaaggct ggcgacacgt 29460 tcaaatacgt gtgtgattgc gacctgttcg gcaagccgat catgagccag ccgatcctgg 29520 caagtgatga tgcaattatc atcggcggga ccgtgatgat cccgttcggt ggagacgagg 29580 gccgttgttt tatggcctct tttggtagca aagtggagaa aattgatgga actgactaat 29640 aacgttggcg gcaccaccct gatgtatgca atcgacgaca ccgccgccgg gcacaatgtg 29700 ccgtccatga gcctcgggca gaccgatgac ggtcatccga ttgtggtgaa agctgtgttt 29760 gctgacgaag acagcgccaa actgatggct aaccacatgg acaaacaacc gggtgcccac 29820 attggtcgat tccaggttgt cgctgttgaa ctgtggcgtc cgacggacat cgaggtatac 29880 gttcgtgagc gtatccgtga atctaagaag ggcgagggtc ctaaactggt actgccgggg 29940 agcctgtaat gcacaaggtt gaagtgacga aagaagactt tgcggccctg ttgaagcagt 30000 tcacgggagc atctggctgg cgtcgttcta atggtaaggg ttcattcaac ctgaaccaag 30060 agcaggacga agacaaacgt cgtaaattga agtatggcga cattgtgttt atcgaaaagg 30120 ttgacaccga ccagtaagtc atgtaatctg ccctcattgg aaacagagag ggctttttag 30180 atgaaattta cgattgaaga ttttgaggtg acagttctgg cgggcaagcc cgagatcgtt 30240 taccacaatg acggtaacgg caattaccgt gaagtgacga ttacggcccc tgaagaattg 30300 attcacccgg cgttcaaagt ggagttcgaa gagtacacaa aacgctgcat taagttgcac 30360 cagaataagg tccgtctgca cggtggattc tgtgatacga tgcgttatgt accgcccaga 30420 aaaatcaagt cgaagttctc tgcatacaaa ttccgtaagc aggtcgaaac tcgggttggt 30480 gatatcgagg aggcgaagga cacttgggac tacaaccgag cgccagcggg agcggtggcg 30540 acagggaagt ttggggacct gaaggcattt ttggagatgc gtacaggccg agaactgaat 30600 tgcatgggga ctggagaagg caaacatgtc aagacgtcct gaatacaaac cgaccgatta 30660 tcgctatcag gaagagatcg acaaaagttg gaacccgcaa ctgaaacggg gtgatgactt 30720 ctgggtcttt aaccaaaaga cggggaaggt gtgggggccg tacaaattca gccactacac 30780 taaccttggg gcggtcggtt ggtgtgatgt tccatacaac agcactgtga aagttcgtta 30840 cagcaagggt gaaattgtgt gcgccactga ggcgctcgct attggtcgcc aactcatgga 30900 gaaagcccga gagtggtccc ggacaacgct gttggctgag gacctcaaga gggaagttga 30960 agctctgtct ctcaaactga aggagattga aagtggaaaa tgaaattgat attatggatc 31020 agttcaatga agttgctgac cgtgtggaaa aagaactcca caatgaggtt caggtaatta 31080 tcgaccagaa cggggacggc acttggcacg ttgaggttca agaacgtgct gaggaagagg 31140 atgaagagga ctgggcgtgt cattgtgttg acggctggtt caccatcaac gaaaagcgag 31200 actgcaagtc ccctgatgag gcggcgcagt ggcttgaaaa tcaatacctg taggagggtt 31260 tatgttcgta tacctgaacc aactagcgtc tgaagaacca atctgcctcg atgttgcaaa 31320 tatccgggag tttcacccgc aggtgaccga agtagaaggg aaagaccgca agggaaccct 31380 gattaccctg taccgtggcg agccgtggat cgtcaagcag tcttgctatg acgttctcca 31440 gatgtgccag aaggcgaagc ggggtgaact ttaatatgcc ttgtggtaaa atgacatacc 31500 gtgtgggcga tctgatctct gcggcgcagg ctggagaggt aaacgtgata gcccactgct 31560 gtaactgcat gaataccatg aagtctggta ttgccccaaa gattgcgtca gcttttccgg 31620 aagcgtggac ggcagaccaa atgaccaccc gtggtgatcg ggctaaactg ggaactttgt 31680 cctcggcctt ctgcaaaaat ggtttactgg tttacaatct gtacggtcaa tacaccttca 31740 ccgggcgcag agagggtaag atggacctcg attactgggc attgcgttcg gccctgtctg 31800 caatggcagt atctctccat gccaagtgtc gtactctgga tatgagcacc cacgaccttc 31860 gtatcggact accaaaaata ggggctggac tcgcaggcgg cgattggggt atcatatctc 31920 ggatgattga aggtcgcctg agagacttcg atgtgaccat ttatgttctg aaggagagtg 31980 aaattgtcca cgatcaaaat ggctgagttt gcccgtaagt acgggaagaa gtacggcgga 32040 aatctgtgcc tggagggatg taaatctggg aatcaggtcc ttagccacga aaaactcccg 32100 gaccttcagg tcccggagaa gattgtcaca ttgacaggtt atatcgagtt caatgaggcg 32160 aagcgccgga agacaggtaa ggatgggacc caagtgcgta ctggctacgt gactggcctt 32220 gccgtccatt ctgggcattt gtatgcgatc accgtcgatt ccgtgtaccg tatctacggc 32280 ccttggtatg ccgacgtgat caccaacgac cctttcctca attctctttg ggaagatgtt 32340 aagaagggag ttctcgctca agcctccaaa aaggcttgac ttctaaaggc tcagtgataa 32400 actgggcctt tctcatttag gagtttggat gaaaaagagc accgctgcat tattcgcagc 32460 cagcgcttta tctttcgcct cagtacctgc cttggcaaaa gataacgcac cgaagaaaaa 32520 gctcgccccc attgtccatg tttgcaagaa gaatgactcg tcggtcaatg ccttggcctg 32580 caatatttat ttcgagtcac gcggggagaa tattagtgga caaatggcag ttgggtttgt 32640 tacgctgaat aggctgaagc acgacagcta cccggggtca gtgaggaagg ttgtgtatca 32700 atcctcacaa ttctcttgga caatggcgag gagtgggtat gccgtaaggg accgggaatc 32760 ttggatggtt gctcaagatg ttgcaaaatt cctttacaag atccggaaca atgatgtatt 32820 gtacaacaag ctggacccaa cacatggtgc gacgcacttc cacacgaaga aggtcaagcc 32880 atactggaga aagttcttta cgaagacagc cactatcggc taccatgtgt tttataaacc 32940 aaaagaggaa aaggcgacat gagaaatctt atcattctgt tcattacttt tatgcttgtt 33000 tggatattcc cggcccgctg gggcgcacag ttctttttcc ctgagatgat ggcacagttc 33060 tcatcccagg ttccgtattt gatgggatgg ggagccttga tgttcctctt ggcaactgta 33120 atcactgctt gtttggctca cgattaaggg gagtgtatgg aagagaagtc tgtttttctg 33180 gtatcgtccg atgcgggtta tggtcaaagc gttcgcgcca aagttgacga tccgcaggcg 33240 gcaacactga aggccagccg tttggcaaag aaaggccaca cgaatattga aatcagtgag 33300 gccaaaatca ttggcaccac caaatttgag gcagaggcgt aatgggcaaa acagtaattg 33360 gtaacgaaga agcaatgcga gccatcatgg cgggtgataa cgtgttcatc accgggccgg 33420 gtggctctgg caagagcttg atgattgcca ctctgcgtga gttcttcgct gacagtttcc 33480 tgttcgttgc accgacaggg attgcggcgc tgaacatttc agggatcacc gctcacaagg 33540 cttttggcct gacgtttggt gttaccacca aggaagatta caaagcgaag tccaagaagc 33600 ctgcgatgct gatggcatct aatgcgctgg acgctatcgt gttcgacgaa atctccatga 33660 ttcgtagcga caagctgcgg gagatcgaca tgaagctgcg ttatcaccgc aaggtaaaca 33720 agcctttcgg tggccttcag gtcatcatgt ttggcgacgg tttccagatc aagcctgttc 33780 tgaagcgcga agagacggcg atgttccgtg aactgcacgg caatgaaatc ccgttcggtt 33840 cggatatttg gaaccaactg gatttcacca atgcgtatct tccgaaggta caccgccagt 33900 ctgatcctgt gttcgctcaa cacctgaaca acatccgtgt gggtaataac gtcggcgcgg 33960 cggttgatta cttcaaccaa cactgtttcg gtccggccct gccaggggcg gtgactttaa 34020 cgaccaccaa caaactcgca gaggaaatca accagcggga gtttgagaag atcaaggccc 34080 agcctcatgt gtttgaagcc aagatctcgg gtgagttccc ggagcgtccg gtcaacgaag 34140 ttctgaacct gaaagaaggt ctgaaggtca tgattgttgt taacgacaac gaccagaaga 34200 aaaaggagcc agattacgtt aacggtacgg tggggatcat caagaaaatc gccaagactt 34260 ttgtcatcgt ggacattgac ggaaagccga tccacatcgg caagtacgag tggaccaacg 34320 tgacacaggt cccggaagaa aagatggttc aggttgaaaa ggaaatcgaa ctggaagacg 34380 gcaccaagca gactgtcaag gtgatggaga agcagaccgt actggttgac aaagagatcg 34440 ggaaatactt gcagttcccg ttcaaattgg gctatgctat caccggacac aaagcacagg 34500 gcctcacgct gggcaaagtg aacattgatt taggtttcgg cacctttacg ccgggccaag 34560 cctatgttat gctgtcccgg gctacgagca ccgaaggtct tcgcctgatg aagcgcctgc 34620 gtgtgaagga cattattgtt gaccaagctg tccgtaagtt ttaccatgag acattccctg 34680 agatttttgg aggagaagca tgaaaaaatt ccaagagggt cagagggtaa ggtttacccc 34740 tcgtgatttg aaaaattgga ctgacttcta cacgccggat ggtgttgtac ccggtgcgga 34800 gggtgttgtc accagcaaca agtttgatga tgatgtcctt gttgacttta cacgcccaga 34860 cggcactgta tgtaaagggt tcttggcatt taccgccgac gtggaggcac tgaatgatta 34920 aggactgttt cggaatcgag gcgcgtgtgg gtgaccacat cgccttttcc cggggtaacg 34980 ccggggccaa gccattcgag atggcggtga ttacgaagat gacggagaaa tccgttttct 35040 tctccggtaa aaccgggtcc atgtaccgtt ctgcttccga cgagcttcgc cggggagagg 35100 gttgtttcgt aattgacacg acgaaacgag accccggcaa agagggccgg gccaacgctt 35160 gggagtcgtt tgacaaacac gtccgcaata ttgttggtcc tgcggcagaa cttcgtagag 35220 aaatctccga agagcttcac aaagacggat gtgcatctgc gtgggttcac catgcgtact 35280 ttgttgatgc tctggtggaa aaatatgagg agtctctcaa tgactaaaga cctgagcaat 35340 ctgtccatga gtcaccttct ggccctgcgt gatctgggcc gtgatgatct tcgccgtgtg 35400 gagaagaaca aagcagaact ggaagaaatg ttggcggcaa ttaagctgga gatcgaagtc 35460 cgtggaaacc gagcagcaga atagggtcag aatgaccgca gaacgccact gggagcgttt 35520 tgacgaggca tgtcatggaa acgcacagca aatggcagaa atcgccgaga gcgcgattga 35580 ggagctttta cagcgtgagc gtgaaacccc tcgaatccgc ccggatttgt tcaacaagct 35640 ggtgatcgac atcatggata ccgttaagac ctatggtgcg acccagcagt gccggaccaa 35700 gctggtcgga gttatctcca agtacgttgt gccggaccat tcacatacga ggccaagatg 35760 aaagatgtcc gttgtttggt gaagaagggt gaaggggatt ttgatcgggc cgcagggctg 35820 gggtatgcca tcaacctgaa ctgtgctgag ggttatatgt ggctcatcta tcccggaagt 35880 gcctgcacga cccctgaacg tttactgaag ctgttcgaaa agcacctcgc agaggatggt 35940 ttcgaaaagg tatctgcggt ggagttccga tgaaacctgt ttttgcttat gagatcaacg 36000 acgttgttct ctttgaccag ggcaatcagc gctgggatta tcgcggggaa ggtaaggtcc 36060 tgcttcaaca ggaatacctg acctgcaatg cttatctggt cgaaatgccg gatggagtca 36120 agacacaggt cagagaagac caaatttccg gtaagaagta acagatcagt taagaaatca 36180 tcaataagga gttatcatgc gtagcacaac tgtagagttc ccatattttc acaaggattt 36240 cggacaggaa gttgtagtcg aagccgaagt gatgctgtat aagggcattc ctaacgccgc 36300 aagcgactgg gatgcagagg attacctcga catcatttcg gtttccgtct acttcgaagg 36360 tgatgtgctg gacattgaca ttccccgtca agtgatctat gctgagatct cggctcagat 36420 tcgtgacgcc gaaatgtcac gtgcgttttt ggaagaagac gggggtttct gatggttgaa 36480 gtaaagattt tggtttacac caaccccgca gagttgcaga ccaacatcaa gaaatggatc 36540 aatgagtttg actattcact caaaggtccg gtcactgttg gtgtcaacat gcacggcaat 36600 acgatttacg ttgcaacgtt gactaagggg aagtgtgagc acatttggtg aaggattgtt 36660 cggtggctac aagacggggt acaccaaccc gaaggcgtca accacacggg gttttggagg 36720 cggagtgcag cgagttgaag aaacagttga agatgtaaag acgtatccct tcaaggcgat 36780 cccggagcgc gggattgatg agaagacggc tgaacgcttc ggtattcgca caaaactgtc 36840 tgctaaggac ggtatgacgc cggaagcgca ttattttcca tatactttgg aaggaaagat 36900 cgttggcttt aagaagcgcg acctgaccat ccctaagcag cagaagggcc atttcagcac 36960 tatcggcttc caatcggtag cctgtgatct gtttggcctg gagggtgcca acaaatcgtc 37020 caaacgtatg atcttcatca cagaaggtga atacgacgca gcgattctgt ggcaggttct 37080 ccgcgagaac tacaaatcga aggacggcaa tgcccgtaag cctaacttca cggttgtgtc 37140 gatctccaac ggcacatcca acgcagtaca gaacctgtct cagaagaaca actataagct 37200 cctgagcaag ttcgaagaga tcattgtggc tttcgacgcc gaccgggcga acgaaactga 37260 gaaagccaag ggtatcatga agggcaaaga tgccgtggcg gcagtgtatt ctgtcatccc 37320 acagatgaaa gtcgtcgata tccctgaaga catggaccct tgcgacatgt acaataaggg 37380 cttggcgcag cagcttttct gggcagcgat gaagccgaaa gagtacgtgc cggacggctt 37440 tgcgacctac tctcagttcc gtgagaaggc acacgagatc cctaaactcg ggaagaaatg 37500 gccttggcct acaatgacgc gcctgacgtt gggccgtcgt ctcggggaag gtatgttcgt 37560 cggtgccggg gttaagatgg gcaagtccga gtggcttaac cagcttgtgg aacacatcat 37620 cggcacagag ggccgtaagg ccgcagtgtt caagttcgag gaagagaacg agatcacgtg 37680 taagaaggtc gctggtaagc tgtatcacaa ggatttcgtg aacgcagaga aggtgatgat 37740 cccacaagag gacggaactt atcgggacat ctggggtgag gaagtcttcg agggccagcg 37800 tggttacttc aaacaggaag aactgattga ggctaccgac aaggtaggtg acaacatcat 37860 ctactacaac aactacggtc gtgcagtatg ggacgaggtt aagggcgcta tccgccatgc 37920 ggttatcgta gagggttgtg aggacatctt cattgacccg atcactcgtc tggtgcaggg 37980 tatgtctgca tccgaagcga acgtggagtt ggagcgtttc tctgatgaaa tctccaaact 38040 tgcgaaggaa ctgggcttct ggtattgttg cttctgtcac ctgaacaagc cggagacggg 38100 taagcctcac gagttcggtg gtcaggttca gtcggcacag tttgctggtt cccgtgcaat 38160 gatgcgtaac acctactaca tggtaggcat tgagcgtaat aaggacccag agcttccaat 38220 gaagcagcgc aacacctctt ggtttgtgct gttggatgac cgtaagcacg gtcgtgctgg 38280 taagttcccg gtgttctacg acatcgacac aggtgattat ctggaaccgc cagaggggtt 38340 cttggacagt ccatacgaaa ctctccgtga gtggtatgcg gtccacccgg aacatgatcc 38400 gagcaacgat tcttatcagg agccgacggg aagtgcagca caacctctgt ctccggagaa 38460 ccaagataag accggatctg acctgacgga tgatgacatc cctccggcgg acgagcagtt 38520 cctggcagca caggaagagg ccaaggaaca gcaggttgag cagcaggatc tgggcctgga 38580 agttccagaa gttccggaag aggaacagac gaaggaggaa actccggagc ctgttcaaac 38640 aacacctgtc gttatcccag acatcccgga agtcccggac atgcctgatg aaatggatga 38700 tgactgccca ttttaattaa cggggcttcg gccccattta cttcgaggtg attatgatta 38760 ttgaacgtca acgtggtggt gaacgtaacg attttgacat cccaccgatg aaaaatggtg 38820 cggttgtgga catcactgat attgtgacaa ccgcttttga gaatgcgatc ttgcagaatc 38880 ttggtaactc ggtgaaggtg accttctcga cgtctgtgcc gaacgactcg cagttctacc 38940 cgctggatcg gaatgtgcca caatatctct atccgcatct gtcggagttt gttcgtaccg 39000 gagtatcatc gactgaactg cgtaacttta tcgcccaagt aatcactgtt caggcaaaca 39060 acgttattca gcgtgtgcag gcgtcaggtc actcctgttt ggagttccat ccggcagata 39120 tggtcatgta cgcaaacacg caggcccgtt cggtatacgc attcctgcgt cggcacaatg 39180 accgtttctc tatcgagaca gaaacctctt tccgtgtatt cactccggag ccggaagttg 39240 ctgacgcaat gccggaagcg gcggcagaat aataaaaagg tcccttcggg ggcctttttt 39300 cgtttgacac cccgcaataa cttctgtaga cttcgaatat tactcaagag gaggatatca 39360 tggtaaaatt aatggcccct gtagttcgtg acattgttgc agaacgtgaa gggatcgaat 39420 ctctggttgg caagcaggtt gaagtcgtca agaagatccc taagcacagc cgtatcacgg 39480 tggcaactcc gggcatgggt cagccaaaga ccttcccggt gaacaaagac gtttacctgg 39540 tgaatgccga gaagtcccgc tttatgtcca ccaagggccg ctgtggccca gtgatggtac 39600 tgttccgtgg ttatgagggc aatgacattt attctgcgga ggaacttgga ttatgatcac 39660 atttttcatg acgctgctgg cacactgttt tggtcgccgc cctgtggatg aggtgatttg 39720 ggtgattgca atgatggttg gtatgctgga gcttctgatc gccgggctgg cgatagggga 39780 gttgctgaag tgattgacaa gaacttcgaa acacattaca gtgtcaagtg ccggatgtcg 39840 atcggtgaca actatcatgt gtgcggttgg gccagggacg aagagaacct gaaggaactg 39900 gtcgagcact tgaagaagac ttggcggtat gttgagtaca gcggctaccg ggcgcgggtc 39960 tgtgaaccac aaccttcaaa gaggaaacca cgtaaatgat tgacgattca cgcaaggacc 40020 cttgcaacct gcacgagggg acgttgttct ctggcgggta tgccttggtc tacgccggac 40080 caaaagagtt ccacgggaag atggtcttcc tgagcaaggc cgacgagttc ggttggtggg 40140 agtccttcga ccgcaaaggg gatatgctga tcctcaacgt agattggctg atgcctgtct 40200 ccgagccggg ctgggacaac aaaacagatt tggacacttt cctttatgaa gcgaggtatg 40260 gatcgtgatt gacagaatta ttgggctgat gacggcggca caggattggt ggtggggaat 40320 cctgaaaccc atcacttcac cgacaaccct aattatttac ctcatagcct tctcggcagt 40380 ggcgctgttc tttatcttac gggatcagta caagaaatgt gaagccatgt tcatcttgga 40440 gggtattgag gtgattgtgg ctgggtttat cggtggctgc gtcttggcat cggtatacgg 40500 tttaccgttc atattctttg ccaccatcgc ggggttattc ttgatgggcg tcttgacggt 40560 attccagttc ttggttgttg tatctgtgaa aggagttaaa cgatgagttg ccgagatatt 40620 ttggacctga tcttcctgat taccaatggg tttttcatgt taactgcggt gtactggccc 40680 aagatttatg gcgacggcat ctggagccgg gtggtgttta ccaccaactt tatcgccgtt 40740 ctgatgtctt ttggtggact tattgcgagg ggtgcgctat gatgggtggt acagtaagag 40800 tggtcagcac aactcgcaag gttccggcgt tgacggttaa cggacgctac caaatcttcg 40860 tgcgccaggg caaggagttc atccttgact ggtatggcgg ctatgtacct gtgaaagagg 40920 ccaaagaggc agggatcatt ttggagagaa tgcaatgaaa tattgggtcc gtctggataa 40980 gcagcgcatc atggcagatt acgacgaaga ggatctgctg gactcttccg gtatagattc 41040 catctatctg atccggcagc ttcgttttta catcgaacat ggcgaaaaca tgatcgcggc 41100 agatgaagac gacatcctat tcccttacca aaaagttaag atgcgcgatg gtttggggcg 41160 tgatgtgttt gtcccttgca caattcacgg cgaggttggt gatgtctaaa gtcgtctgga 41220 gcctgtttga tgggtctggt ttcatggggt atgagtacgc cctgaatggt tggaaggtgt 41280 tctgctttaa tgcagacacc gctgatcatg gtcagtatga actgcgccgg gagcacccca 41340 acatccagta tgtgaattgt tggatcgatg aagacttcga agccttcgtt gcgaaagcgg 41400 gtattccgaa gccggacatt atctttgcat ttccaagctg caccaacctg tctgtagccg 41460 gggcgaagca tactcggacg aaatcggagt tgatggagtc gatagataat gctaaaatgg 41520 tagagcgtgt tgctaacgtt tttggctgtc cttggatggt cgagaacccg gtgggcaaga 41580 tgagtacgct gtggcgcaag ccggatcatt atttcgaccc ttacgagtat ggagggtact 41640 tgagtcagat tgaaggctcg taccacccaa aaatgccgta caggaacgcc tacacaaaga 41700 aaacttgcct ctgggtaggg aatggctttg tgatgcccga caagaagcct gtgcctcata 41760 taggctgctt ctggggctgg aagtttttag gcggtaagtc ggagaggacc aaacagctac 41820 gatcgctgac cccggagggg tttgcccggg ctgtttacca cttcaatcat taaggagtaa 41880 aaagtgcagg aaatcctatt ggcggctggt gcaggcggcg gcatgaaggg agataaaact 41940 ctccttctcc tgcaagcaga caagggaggt attgatgttg ccaagcctac ccggcaattt 42000 tcaaaagtag caacagtagg caccggaagt tacggaaaat tcaacaacaa atactttgac 42060 tgtactacca ctgccagcta ttttgagatg atggcgacag agaaaacaga tctcgtcttc 42120 aaaccagagg acgagttcac tatcgaattt tggtcaagaa actcttcagt tgcctcttcc 42180 tggtggatgt attttgggac aggtactcag agttgcctca aggtttacca aagtgtggtt 42240 tatcttcagg accagaagaa ttatttgagc tttagtacca actactggcc tctttccaac 42300 tggacacatg tggctattgt gagcaaaggg ggagtcacga agttatatct gaacggtaaa 42360 gtggcaccaa ccaccacgtc ctttgcttcc ggagggtgga gtgacgtaag ccgttttgca 42420 agaatcggct tcggcgaaca aactatgttt ggtgggcttg accaattccg tatcagcaag 42480 gtggcccgtt acgacggtaa tttctcggtg ccgacagcac catttgttct tgattaatag 42540 gcaaaaggta ggagtattca tgagcgaaca acgtgtaact gtaggcgtta ttgcactgtt 42600 agaccctcca ctgggcaatc tacacacgga ttcttggcaa gaggtttccg acaggcttga 42660 cgagatcgat tccaagttga agatcactta cggtggtgat atggtcgttt accgtcatga 42720 agacggaccg tcgtatgatg caggcctggt gatcttcgac cagaacgata atatgttcgg 42780 caacctgcaa gaggaattac ggcaggcggg cctgttcatc gtggaagggt ccgagaaaat 42840 gtttgtcgat aactggtatg acgggtgcga tcccccgcat atcgccataa cccgtgaaca 42900 tgccggatac gacaaggagg ggtccgatga ctgatattaa accatgccct ttctgtggta 42960 agcaacctga ttactgggaa gatacccatt atcaagataa gcacgtaata acctgcggct 43020 ggtgcggggc agaaaagaga tctgagtacg ggtacgattc cgtgctgagg gactggaaca 43080 ctcggtacaa cggtgcaggc gagttggtgg aggaataatg atcgagctat taatggcagg 43140 aggaaagaaa ggggtgcctg tagacatgga tgactttaat gctgacgagg cattagtgtc 43200 ggcatcagcc gccatgatgc cgggtcaatc ccgtggcggg actttgggag gctcttctcc 43260 taccagcaca gcagccgtcg cgatcgggta ttggaatgga aaaatctacg ttctgaaagc 43320 agatggtttg cacaccttca atgtgagcga catggcatac tcaggcatgg tccctggtgt 43380 ggctggaaga accataccgt taaatagcgc cagttatctc cataagggag ttctttactt 43440 cggacaaggc aggacaaaga caggggtaat cgaatccctg aacttggaga cgggtgtgta 43500 caaaacttat ccctccttgc cttatgatta tggggctatt tacgtagatg acgattacgt 43560 gtactctatt gccaatgtct acaagtccac cgggttttca tcgaatgcga tccgcagatg 43620 ccggatagat ggctcggctt gggaagacgt aattctttcc ggtgcttttt ctcacggggt 43680 ctctggtggg gacgccactt tgatgggcgg gtttgtttat ataaccggag ggggttcaat 43740 aaagaacaca agcgagggcg acggctctcc atatgtggat gtcctccgtg tggatttgac 43800 aaaccttcag atctcaacag tacaaattac aagcccggga tttacccctt acgatatgcc 43860 gtcttgcgta tggaaagggg ttatatatac agtccaacaa agcagtacgg tacttaacca 43920 gttcgatatc ttgacaggta gtaaaacaac agcttctgtc ccaacaaacc agttccactg 43980 cccatgttta agggtgggcg acttgttctt ctggctatct ggttatagtt caaagacgat 44040 ctatcaatac aaattaccgg gaggtttgtg atgggaaaac ttcgtaaacg ttcataccct 44100 gccttggcgg aagccgggtc aggcgggaag tggttcacag gatgggaggg atttgctttt 44160 acaccaagag cagaagaggt gtatgctgcc cgattcccac tctgtgactt tgttcatatg 44220 ttccggcccg gttcggaaaa caaacgtgta gaggagatag agcacgaaac ccgcaagatg 44280 tctctccagt tttctcacac agagaaagga aggagttctg cggtggcggt ctttgaagac 44340 aaagagggat ttgaatacaa gatctccttc agtagtctgg agttgattct ccaactcctt 44400 cacagaaaac gtgatccaaa cacgaagtat gacatctcgg aaacacgtat tcaaggttcg 44460 gggacttggg tagaggggac cttcatgcaa gtcaagaagg gccagaacta cttcatcgag 44520 cctgttgagg tggactgatg ccggattggt ttggtttagc tttcttgggg gccgtgggcc 44580 tcctttgtgt tgtcatgtat attctcgaag agagggcgca acatgcttga attgttagcc 44640 gcaggcgatc aaggaaagat gggtacaggg gggttcaagt atttggacag cgtgactgga 44700 ccaacataca cccaagtgaa cgatgcgatg ggattcgtgg gcggaacaag attagctggc 44760 gatacctaca tgagctttct tgaggaagat actggagaca tttggctggt tgcaaaggca 44820 cagacgattt ataatcttgc ccacaacacg ctggatgacg ccggggcctt ttctggcaaa 44880 gtcatcacaa tcgccgggga gcagtatttt tgcagaactg taaaaatgac aacccgtgga 44940 ttcaccaatt ccgacttgcc aaatgggggg agtgtggtgt acacggcaac ttcaactttt 45000 gcacattctg agttcaacaa tatcttttac caagttcttg ggctgaatgg tcagtcggtg 45060 gaaggcatag agtacggaac ttctgcaaaa ttcagccctg cccaactggg ttttcatgcg 45120 ggacagggtg gcgctctaat cggcgcagag gttaatcctg atggatacag ggtggttcag 45180 gccgcagggt atacctcccg tttgagtaga aaagttgtca gtgtttatca cggctggcga 45240 ccaatgctga ggaaaatcta atgaatgact atcgaggaac tcccctcaag gagggggata 45300 gggtagcaat ttattggggc tacaatgagc ttcagggcgg aattatccgc caaataaaag 45360 gcaggaaggc caaggtcgag gtcttgggca ccatcagtaa gtggaaagat ggaaattgca 45420 tggtcaagct gccggattga taggttgatc tttacgaggc attgggctat cctgagtgga 45480 ggtatgccaa aaaggagaga gtatgaaagg gttgtttgta ggggatgcgg aagccaacgg 45540 tctgctcgaa gaagtagacc agcttcacgt gatcctgttc aaggagtata aggtcgataa 45600 ctggaatatc ttcctcgacc ccaaccggga agggtacgat caggcgttga aatttattca 45660 atcgaagggc aagaatttca aaattcgtac ctttgaggag ttgaatgatt ggttcaagac 45720 ggagcctaaa tccatcgcga tccacaacct gttcggctac gacctgcgtc tgtttaaaaa 45780 gatgttggga acacagtatg atatgttcgg cgacccgaag tgcatgggca ccctgaacgg 45840 caagcaggtt aacctgttcg acactctgtc aatgtctcga actctttatc cagaccgacc 45900 gcttcctcgt gggtgtccgg agatggtaaa gaacccggtc accgggaaga tgaaacctgt 45960 cggccctcac ggcttggaag cgtggggtat gcgtgttgcc aaccagaaag tggcaatcga 46020 agattggcgc ggactaccgt tgtggaagta ttgcgatcgt attatcgaag acgtcatcat 46080 caacgaaatg gtgtggacgg ccctcatgga agagattgct tgtgacggaa agttggttga 46140 cgacaagcgc ttcatgtaca aggacgaaga gaaggacaag gacgccaaga agtacaaact 46200 gatcaactgg aagaacgcga tgcgtcgcgg tatgttgacc gattacctga tggttgagca 46260 agagatccaa ggcgtatcct tcgacgaaga agctgcactt aaacttcgtg atcgcatcga 46320 tcaaatgatg cgggagatcg aaggcgatgt cgagcctcac cttcctaaaa aggagatggc 46380 gaagtctgca cagcctacgt tcccatccaa accgttcaag ggcgatggat ctttgtccgg 46440 aactgggtgg aagtggctgg agaagttggg gtataatgtc aactgggagg cactcgactt 46500 taagggtccc ccggcgaagt ctttcaaggc cgatggtagc ctgacggcgg cagcaatcaa 46560 gtattgcaat gagcatggtg tcgaggatga ggcagcgatg ccgcagttta tccgggacca 46620 gaacaacaag caaacgaccc tgatccctct gtccccagag gacatggcac gagcaatgtt 46680 ggatctgaag aaccaaacta tgccggacat tatgatcccg atgcgtatcg gtaaccagga 46740 tgacgtgaag aagttcctga tccgtgatgc aggctggaag ccaaccctgt ggcgcagtaa 46800 ggatgtgacg aaggaccagt ttaagaagca acgccccgat aacgaggtcg atggacttgt 46860 tcgtgagtac attgagcagt tggaccaagt tgaataccgg gatcttattc tggaatacct 46920 cggcatcacc ctgaaggcgt ggcaggacaa agagaagatg tacaagctgt tgcgacgtaa 46980 ggcccgtggc ctgcctacgt ctccgcagtt gaaagatatg agcggcctgt gtccgaacct 47040 ggagaaggtt gacggtcata tggcaaagca gatcgtgaag tggctgtctc tgcgtaaccg 47100 tcgttctgta cttgacccgc tcaaggaaga caaggttgac acaggatggc tgaaccaccc 47160 acgactgcgt attgacggta aactcccggc gcgttattct gggatcacca acaccgggcg 47220 acgtaagcac acaatcgtgg ctaacgtccc taaaccagac gagaaggttt tgctgggtaa 47280 agagatgcgt ggcttattcc gggcatctga agggaaatat caggtcggta tcgacggctc 47340 aaacctcgaa ggtatgatcg cagcatgggg tgcctacgag ttcgacggcg gggcatatct 47400 ccggatcatg gaatcaggcg acgcccacag ccgaaacgcc gaagcgtata ccaaagcggc 47460 gggcaagaag gtttcccgtg gtgagggtaa gggtgtgaca tacggcatca tgtacggtgc 47520 ccaggcagcg aagatcgcct ccatgttggg tatttccctc gacaaggctc aggcggtaat 47580 cgacgcattc tgggatagca acatcggtct gaaaggtcgt aaagaagcac ttgagaagtt 47640 ctgggagttg accgggaaac ggtttatcta tggcctcgat ggtcgtaaga tttacacccg 47700 ctccaaacac tccttgctga acgcatacca gcagaacggc ggtgctgcac tgtttgactt 47760 ggttggtatc ctgctgcact ggcagttggt taagcgtggc tggtatgatg aaggcgtccg 47820 ccgtatcatc tactaccacg atgaatacca gcttgaagtg ccgcacaagt tcaagaaaga 47880 atggaaattc aacgacgagg caaccgcaaa agcctttgtt gagcagtgcc tggcgaaagg 47940 tctggttctg gatggtcact gtgtgaaaga aggtaagttc aagtgtgatc cggacgagaa 48000 cggcgtgtac acgattattc attgcccggt cgggcagatg gtcgttaagt gcgtggagca 48060 ggcggctaag atgatgaaat ctccagtcca catcacaggt gaatacctga cgggcacaag 48120 ctggggagat tgtcactaat ggctattttt acacatttgt ggtaaaatgc ttgtattccg 48180 ggggcttcgg ccccttttct tttagggggc aatatgcaag agatgttggt actgccggga 48240 ggcggagatc ctaaaaagta cagcacttgg ttcgagttgg acttcaccaa gtatcccttg 48300 gggacattct caggattcac tgatcgcggg ataacgttca acaggaccgc cggaacatcc 48360 gcgacggttc ttgacgaggg cctgccagcg atggattttg ttggagatgg ggcattgagc 48420 ggagggacca ccccagacaa gctgctaaac aatgactgga agatcaccat gaatctgaag 48480 gttaaggcgg cggcaacaaa cccaatgatc tttgtgggga ggtctattca aggaaacgga 48540 tctggcactt ggatcgccaa catgctgaat aacggatacc ttgattggtg gtataattcc 48600 aacagggtca atgcaggccc agccgtgaca aaaggtgttt atcacgagat aatcttcgag 48660 tctattaaca agagcctcac tgtaactgtt gatggctctc cgacgataaa caacaaaacc 48720 attccagatc ttgcagacgg gacaggtttt aatctcatga ttggtggttc tcaagaccaa 48780 gctctgtatc attcccggtg cttgatgcgc tcaatcaaga ttgaaaccaa gaaaagataa 48840 ccgcccttcg gggcggtttt ttattacctg aagaaaagtg ttgacgtgca gaatcattct 48900 ggtaatatgc acacatcgaa acaaaggagg tcatccgtga ataaggggga gtttcaggag 48960 tggaaaaatg aaaattcgtt cgaataaatc gtttgacacg ctggaagaaa tagtgcaata 49020 tcttggtgac ccggaacgag ttggcaaaga caacacgtgg gtggcaaaga actattcgct 49080 ggcacaactg ataaaagatc tgacgaagat aaaggagacg gattatgtca tttaaagcac 49140 tggcccaagt gactgttcac cctgacggga ccatcactgg aagagttatt aatcacgaaa 49200 tcccgggtgt tggtctggtt gagaacgggg tttatgaact cggactcgaa acattcagca 49260 gcaagctgga tatgacatat attgggaaag tggagggcaa ggctgatgaa tcctaatgat 49320 ctgctgccaa cagtcacggc aattgtgggc tacgatatat accctcatta cttggttacc 49380 aagggtaaac ttttgtcagg cggcagggtt aaaaccgagg caggtacata tgctgcggat 49440 tctattatcc gaatcctgcc agagtgtgat tatgaatcac aacaggccgt acttcgcaca 49500 gtgaaatcgg agtatactgc aaaagaacgt caattacgtg ttgacatcct gaagcagaac 49560 ggtgtaaact tcttaccggt tcgataacaa cctaattcat taactgagga gattcaaata 49620 tgagcactaa caactatcat aacttcgtac tggctatcca gaaacaactg accaaaatgg 49680 ctaaaactgg cctgtttgaa gtggctgctg acaaagacgt cgtgtggaac cattatctcg 49740 attcattccc ggcgggcacc aacctgatgt tccgtgagcg ccgtgagtac gactgcaact 49800 gctgtaagca attcatccgt gatgttggtc gtgtggtgac cttcgttgac ggtaagcgtg 49860 tctccatctg ggacatcaaa gtgccgggtt actaccaggc ggtggttgac tcgctgtcca 49920 aactgatcca tgcagcaccg atcgctgacg agttcctgca cttcaccacc aaagtgggca 49980 cggcacaaag caacgttctg gaaaacgatc aggtcgtgac gtataaccac ttccacgccg 50040 ttctgccgca gaacttcgtg aagccaaact cagatatcgc aactgccctg agccgctctc 50100 gtagcaacgt gcaggttctg gagcgcagcc tgattgaact gactgccgac gcagcagaaa 50160 cgatcatcga actgaacgca cagggttccc tgtaccgtgg taacgagcag atcgccagcg 50220 tcaacctgtt cctcgacctg aagaacaaat acgatgctct ggataccgca ggtaaagaag 50280 cgttcgtgtg gacaaacggc ctgaagctgg gtgagcgcgg caagttccgt aatactgcga 50340 tcggcaccct gctggaagat ctgtccggcg gtatggatat cgaatctgcg gttaagcgct 50400 gggaaaaggt tatggccccg gcaaactaca agcgcccaac cgcgctggtt actgaagcaa 50460 tgatcaagaa agcacaggag aaggttcagg aactgggtct gatggactct ctgcctcgcc 50520 gctacgctgt actggaagat ctgactgtga acaacgtact gtttgctgac cgcaacaccc 50580 agaaggccac agatgtgttc ggtgacctgc tgaaaggcgc gaaaaagcct gttaaaggcc 50640 cggctaacgt ggaagagatc tccatcgaga aattcatccg tgaagttctg ccaacggcaa 50700 caagcatcga agcgctggtt gagaaccgcc acactggcaa cctggtgtct ctggtggccc 50760 cggtatatcc ggacgctcca aacatgctga agtgggacaa caacttctcc tggtcttaca 50820 atggtgaagt tactgactct attaaagagc gtgtgaaggc agcgggtggt aaagtggagg 50880 gtgccctgcg tatctctctg gcgtggaaca atggcgatga cctggaccta catatgcgca 50940 cccctcacgg tgagcatctg tattacggta atcgtcgtgg tggcggtgcg tatctggacc 51000 tggatatgaa cggcatggat aagcatgatg atctgaaccc tgttgagaac atcatcttcg 51060 aacgcgagag tcagatcgaa gaaggtattt acaaggtgtg ggtgaaccag tacaatgttc 51120 gttctcgtga ccgtgttggc ttcactcttg aactggagca tggcaataca acgctggagt 51180 ttagtcgtcc acaggcgatg tctcgcggta gcgatgttga tgtgatcacc ttccgttaca 51240 cccgtaaagg tggcgtggag gttattgaat cactgccaca cagcaagcag tctaaagatg 51300 tgtggggaat ggctaccgag cagttccaga aggtgtctgt gatcatgaac tctccgaacc 51360 actgggacgg tcaggcgatt ggtaacaagc attacttctt catgctggaa ggcgcaaaac 51420 agccgggcac tactcgcggt ttctacaacg agtatctgcg taacgaactg aacgagcacc 51480 gtaaagtgtt cgagcatctg gcaggtaaga tgaaggttcc ggcctctgac gaacaactgt 51540 ctggtctggg cttctctgat acccagcgca acgaactgac ggtgaaagtg actggtgcat 51600 tcacccgcac actgaaagtg attttttaat tgacagcggg gccgaaaggc cccataatga 51660 acccgaactt aattactgag gagaaataca atgtccacta ccaatatctt tgaaattgca 51720 gcacgttcca aaatccgttt cgcttcccca cgcttcgcgt ccctggcgac ggaagacctg 51780 ttcgacctgt ctctgaccca actggacgaa gtggcgaagg gtatcaaccg ccagatcaat 51840 gcagagaagg aagagtcctt cctgtcccag aacaaaaacg ctgtgcgtaa agatctggaa 51900 ctgaagctgg agatcgttaa gcacgtgatc ggtgttaaag agaccgaagc ggcagagcgc 51960 gttgcagcgc agaacaaggc ggcgcagcgt cagaaactga tcaacgccct ggcggaagcc 52020 gaacagcgtg aagttcagtc caagacccca gaagagcttc gtgcggaact ggcggcgctg 52080 gacgctgaat aagattaagg ccaccttcgg gtggcctttt tatttgcaca aaattgttga 52140 cttcctctct gatttcccgc ataatcctct catcgacaac aaagaggaaa agacatgaac 52200 aacaccaaaa tcacgcaggt aagttctgca atcatccacg aaagtgattt catgaataag 52260 tcttcatacc gtcgcttcat gaagggtttt gaccgggcct ttgagattca gcaactaaag 52320 gacgaggggt atattatctt tattgacggg aaaccgatgg acaaggatga aaactttatc 52380 ttcggtgacc tccgggataa gccatgcttc ggcatcggcg atgagcgctg catgtacgta 52440 tggcttggaa gtacgttctg tgcagagact gagaatgtgt gcgtaaccac agaagagatg 52500 actattttca acagcatctc ctatgtttca cccaaaaaca tcaagaaaat gaggaagcta 52560 taagatggga tggtgtttca tcaaaaggaa aaacaaagag cgcaactgct ctcacacctg 52620 ggaaataacc cagaacctgc acatcacccg gcaccacgat gccccggcat acgttgttcc 52680 ggagtcgaag cagacgagtg tgtaccggat ctgctgcaag tgcctgaaga aagagaccca 52740 cgttgtggaa gggcacattg atcacagcaa ggcgtacaag atcttcgggg gttcggatgc 52800 gtaaaaatag taacttttgg ttcaagttgt ggtttacttt catcggcggt ttgattatac 52860 ttggtctcgt tggttacttg atgcaggaat accaagactt caaggacgga tgtgtaaaga 52920 ccggggagca gcggatggtc aactccgtgg tcaacgcccc ggacggtgcc ttcagcggac 52980 tggttgttga agacaagttt gtatgcaaga ccggacgaat tacctggaaa gcccaatatc 53040 aatgaggtga catatgaaat ttaatcgtgg cgaagtacag tttggcaaca agatcgagaa 53100 gccgtcccaa gacgacatca agaacacaca gggactgtgg aacgcatcgt tcgatgatgc 53160 tttccgcttc ggtgggtacg tgacccgcgc cgccctgcaa gcgatgaata tccgtggcga 53220 caaaaagcat atcgtggttg acgtgaagac ccacatgcta atgccgggca tgatccctgc 53280 aatccctggt tggcacacgg acggcgttcc tcgtggcggg aagtctctgt ctccggcctc 53340 aggcgctccg cacatccaca tgcaggaagg tactgagtct cctcgttacc atctactggt 53400 tgtcggtggc gactgcccaa cgaagttcat tgcctcacga aacatcaacc tcgtgacgga 53460 gaacctaccg aacctgtacg ccggaatctc cagtcaggtc tgtgagatgg acgacaaggg 53520 cttcctcgac acctacgacg caccggatgg gcaggtggtg gaatgggact ggtgggaact 53580 gcacaccgca caacaggccc gtgcgccggg ctggcgatac cttattcgtg tgaccgagac 53640 tgactatctt gagccacaaa aagatctgcg gctggttctg cgtaatcagc agcaagttta 53700 cctcccaacg gagaaattcg gatggtgaaa aaggtagaca agggccagaa agaagagtac 53760 cattttgagc gtcaccactt ctatctggac aaacatgcag gcaagcaagt gtgtgcgtgg 53820 tgcggattgg tggcgctccg caacaaagct tccgattggt gtgttgaaaa ggggtgcaat 53880 tacaaactgc acacctccta ccaaagcact atgcggaaat taaccaagca atttgatttc 53940 taaggggaac tatgaaagac ctgtttttga tctgcattaa gctgtttggt ctgcttctga 54000 tgatggcgat tatcaccatt ccggggatca gcaaactgac cagtcaggag tttaaggaca 54060 agtaccacgc caaatgtgaa gaactcgcaa aagccgtgga aagtaaaatc gaagatgtag 54120 aaaaatcttt taaataggag agtattatga tgggttactt tgagtttctg gcaaacaatt 54180 ctgtaacagt gctggttttc gttgtggcgg ggattctctt cggctttacc caaggtcttg 54240 gtgactcttg gaaggataag atggcttatg ccgggattgg tgtggttttc tgggggctga 54300 ttctgtcccc gctgattatc cgcatggact acagtgacta taaacgtcat tacaccacct 54360 gtgaaaaata tgaggccaaa caggccgggt acatcttctc tgacaatcgc tgttacaagc 54420 cgatcgccaa ggatgtttac gtgaaagtga acgaaaccac aaaaaccaag caggagcttt 54480 tggatgctaa ataacagcga actcgttgag aagcagtctg aagaagtcaa tgcttttgta 54540 tccggactgg taggcccgga ctaccaagcg gcgatgaccc aaagattccc tctggcgcag 54600 ttaaatttca tcccggcaga gaaacttccg tatgtcgggg aaggaaaagt atgggtctgc 54660 gactgcggtt gcggggaaag agatcctgtg ttggtccctt atctgacata cagccgcatc 54720 ggcgtagagg gacttatcga agcaaatgtc gaaggtatct gggtgtctcc atgcacccga 54780 ccgggttacg agccagagga gggtgttcgt cactttggga tggacctctg ggattacggg 54840 ttggatgatc ttgctgaccc acatgttcgt ttggagggga attaaatgtt gaaggtactg 54900 accaagtttg gtaaagtaat tgttcttctc accgtactga tcgtactaaa cccacatcgt 54960 gcggtactga ttccgttcag cctgcttgct gcctttggca gtttcttggc ggggaagctg 55020 gaagaccttt gtcagtggac agccgacaag gtggactttg cgaaggataa attctggttg 55080 tttgggaaac ctatcaacaa gaagctggac gaagaaatgt ctgaactgaa ccgtatggtg 55140 gcggaggttg acaagaagtt aaaggccgac cgataatggc ggtccttaac ttttaccacc 55200 tgcgtaaagt aatcccggaa aatgctgtat acataggtcg caggatgccc cacttcgggc 55260 tacagcagtc taagtttgca aaccccttca agctgtcgaa agacgagccg agaggggcca 55320 caattgaacg ctatcgtgtt tggttgtggg aacagattcg ttccgggaaa attaccattg 55380 aagatcttct tgctttggaa ggtaaagatc tggtatgctt ttgtcatccc cacccatgtc 55440 acggagacgt acttgaagcg gctgtgaaat gggcgaggga agtttacaat aagaggagga 55500 agtgagatgt ttgagatttt ctgcgaatgg gatgttggtc aggagggcgt tctgtttact 55560 tcagaggaag ccgccaataa ctggatgctc aacaacgaca atctgaagga ttgtttcgaa 55620 gacggcctta ccgggcaggc gggtattgat gatctgatct ccgctggctt gctgggcacc 55680 aattacctaa ctgttctcaa cgagaaagga gagaaagtat gatcaagaaa tttgtggcgt 55740 ggatcaaagc cctgttcgcc ccggcgaaga tcgaagacgc aaaggttgac gagtatatcg 55800 ccaaacaatc tcaggcccag accgtaagtc aggtacgctt taccaaggct gaaacccgtc 55860 cgctggacga gcctctggaa aagaaccaag tcttggtaag tgcaaatgta gcagaaaaca 55920 ccaagcgcgt ggtgacagtg gaaactcgcc agactgttcg tgaggccccg gtggaaaaga 55980 ccccaccaaa gcctctgaac gagcagctta aaggtgtgga actgccagcg tatgcgaagg 56040 gtaagactct accgccgaag gcgaagcgtc agttcatcaa gcaggctaag atgtccggtt 56100 ctccggcccc aggccgacgt cttgacgata ccccgcacag cacaggtggt catgtcgttt 56160 ctcacggtat tgatcccgta aacgcggcga tgtacggcta tgcagccgga gcgatcctga 56220 gtgacgacac cccggcggcg cagcctgccc cggtggaaga gtgccgcagt tcctctgcga 56280 cctcaacgta cagttcaagc tactcgtctt ctgacgacag ctacagcagt tcgagctatg 56340 gttcttcttc cagcagttct tacgactcca gttcgtatga cagcggttca agttcctcgt 56400 cctgtgatta agtaagtaat atcgggggct tcggccccca tcctgtggaa taacgtttta 56460 ccttgaggag tgatgaaatg agcaatacaa gaaaagttta tccggcaaaa cccgagtggg 56520 ctggcaaaaa gcatccacgc tatggcgacc cggagttccc aaaagtgact cccgaggagc 56580 ttgcaaaagc gcgtgaggtg ttctgggaca ttttcatggt caaacacgac agcgactgct 56640 tcaatgactt ctcccatgca ctgggttgcg atcgtaatcg agcaaagcag gtatactatt 56700 tcatcctgta ccagcgtggt ttcatgcaga accagcagat gcaggagcgt aagatccgtg 56760 gcaagttggc agtgaggatc aagaagtata cagagttcct cgacaatcca gagacaatct 56820 atcagatctt ggcccgggca gaagacgagg ctattgaaga tgaagataaa cgtcgcaagg 56880 gagcaacaaa atgttgatcg cgcctgaaac ttttgttata ttgttcatta tggtagcgat 56940 ggctgggacc cggttcatgt attggaccgt ggttttctca atgacttatg tcgtggggat 57000 catgatctcc acagaacttt cgaaatactg ggggtggatg tgaagaaact gattttatta 57060 acggcgctcc tgctttcagg atgcaatcct ttcgatgacg ggaaaccaga tcattatgag 57120 tcgaagaaat cgctgatgcc gaccgaggta gtggcattac acaaggtgtg taagagccaa 57180 cccaattatg acacaagctg ggtggtccag cgagacggcg aggccaaagg tgtgatgtgt 57240 cgataccggg atgcagatta cacccgaacc agcaatacct attcgatcga tgcggagatc 57300 ctccaaatta aaatccaaga ggggctgatc aaatgacaga gtttgtcgat gtgatgtggt 57360 cgttgttctc aggctcggcc tgggtattcg tactgttctg gctggtgcct tacttcgtgg 57420 cccggaagag agaacaccgg aatacgatgt tgattctggc gctgtcggta ttcttctcaa 57480 tgtggcctgc cctgtgggta aatggcattg cttgggcgat actgctgttt attgctgcac 57540 taactgaacc tcagtatgtg tatgtaccag gccccgccgg gcctgctgga ccagaaggca 57600 aggcaggtat cgacgggaag gatggcaagg acggtgaaaa aggaaaggat gccgatgaga 57660 tcccactatc aaaagcgggt gttttgaaac cgaggagtcg ttgatgcaag aactacttac 57720 ggtcggggag gttgggatgg acgtgcaagg ggcgaagtcc ttcttgatat aaacttttcc 57780 cggcagatgg cgggagatac tgtagtcata gaccagtcag ggaagaatac ctttaccaaa 57840 accgggccgg gcgtggctgt ggtggaaagc aaccctttgg ggaattgtat taatttttcc 57900 tcggggccag cttcttttcc acaggtaaga cagtggatgt tgcgagcgag gctgttgaga 57960 tacgtattct gttagaatcg gcgttgaatg tcgggtcaag caacaaaatg gcatggtgta 58020 ccggggatta tcccgggagt agatttatcc cagggatcgc ccactactct ttgttggggt 58080 cagcgtctcc tcaattgttt tgtgtcgatt ctgacgccac atacaaaagg atctccactt 58140 tcgagcaggc gggggtgata aatgatttta ttttcacaac cgacccttca gcaagaacga 58200 tcaccgttga agaactgact cgtggagaaa aaagagttaa cccgattaac tcttggtttg 58260 ggccggggat ggacttctct tttgggggca gttactacgg tggtgtcacc tatgccccct 58320 tcacgggtaa gatctggaga ctcaaaatac agaaaatgat ttgacaacga attattttgc 58380 tgtaagatgt acccaacatt aactgacgag gaagacatga tgagcatttt cgataacact 58440 tttgttgagc cgggttgggt ttgtgttcag gcacagaaga acattacccg ttgcgacgaa 58500 tacaaatacg ttaacctgaa tctgatgtac gaactggacc accgtcaagt tgaagtaggg 58560 gctacatacg ccatcaacgt cggcgggcag atttctcgtg aggttccggt gaagatgatc 58620 atcaagatgt tcgatgtgtc gggagatgta actgaagatc cgaaagtcct cttcaaacgt 58680 tcactgcggg cattgcgccc ggatcagatg acggaggaga tgattaagga cattaatgct 58740 gcaaacttga tgtttgatca agcagacaaa gaggtgcagc gcgtctattt gtaaaaagga 58800 gaggaaatga acaagttact ggcaggttta ggtctgggtt ggctggcaat cgtaatcgca 58860 tccatcgtcg catggatcac ccacgttgtg gtgtgtatca aaactgcgtc gtggctgttc 58920 ctgattgcgg gcgctatctt cgccccggtc ggtgttatcc acggcgttgg tgtttggttt 58980 ggtgtctggt aagacacctt gcaattctga gtaaatgtgg taacctgttg tcctggcctc 59040 aattctgggc cttgtttatc ctcgaaggag aagtgaatgt ctgatgttaa agtaattacc 59100 gaagtagtta acccgatcga caactctacg gtaagcctgg ctgttggtac tatcgaacgt 59160 atcaacatta aacgcatcaa cgaggacggc agtccgaaaa ttacccccat taacaagggt 59220 ccgaacaagg gtaagactat cgttgccact caccgtgcat ctctgctggt taaaaacggt 59280 gaagatcagg tgtggatcag cttcggcact cacgaagtga agaacatgaa atacgaaagc 59340 cagtatcagg taaaagaagg cgaagtatgg gttgacctga agccgggtat ggcaattcgt 59400 gttcctgtcc agatccgtaa gtggactgat agtgacggca acgaacgctc tgccccggag 59460 ggtaagaaat ccaagatcgt tattaccgac aaatccggtg cccgtgatgc cgccccggca 59520 ggcaatgcag gaggctctca gacgtcctct gcgtcgtcca acgccccagg tggcaaaacc 59580 actaaggttt acggagaaat cctctccctg agcgatacag gcgctgtggt gaaggatgag 59640 aacggtaatg aagtctctgt ggttctgaac aaagagcaga tcgcccaagt tcaggtgggt 59700 ggtcgcctgg cagggcaacg tgctgaggat ggtacgctgt ccggtttcaa agcctatggc 59760 ccgaaaggtt ctgctggcgc aggcgcgggc ggttccaaag gtggtttcaa gaaggacaac 59820 tccggtatgg aaacgggcca tgccctgaac ggtgccctga acctgcgccg tagcggtctg 59880 actatccctc cggtggtgga agtagccaag gttgttcatg atgtcaccaa acatctgaag 59940 ctggacgctg caaatgatcc ggctaacaag ggtatgtctg attacgatct gggcgcgatg 60000 gtgggccatg ctgttctgaa cgccacccgt gatatccagg ttggtgagaa cgacaatcca 60060 gaagagatca ctggtaagct gttggcttat gctcgtggtc tcatgagcga ggttgtcccg 60120 ggcgtatctg catacgtcaa gggtgagcaa agttctggtg gctctgtggc acaaactgga 60180 cagtcccagc ctgatcctaa ggtggaccct aaaccggaga ctccacaatc gactcctgcg 60240 acaagcagca acggtcatat cgacatgact ccgccggaag ttgatttcga cgatgacatc 60300 cccttctgag ttgtcatgat aaaaagaaag gcacccgtta gggtgccttt ttattatgcc 60360 tcgatcggcg gggttgcaaa acgtgccaca tattcaagcc ttgaaggtcc gggcattagg 60420 tatcccacaa ccggatatgc agaggagttt cccgtatacc cccagtacag aatctgcgta 60480 tcgttgtagg cagcaaccca acccagtccg aaggtcattc ccttgataga tgggatctcg 60540 tcacttgccg cagcgacatt ggtccagttt gagagtgtac cgctggtttg gctggtcccg 60600 gaaattctcc cgcttgcttg accagtaact atgaatttcc ctcccgtatg gtagaagaaa 60660 agaccactat agttaggcca agttccagag accgccaccc ctcctgtttt gcttaccaga 60720 ggctccgttc catcaccggg ggtgtatccg tcaggaacgt ccgtaagggt attgtatgga 60780 cttgcatccc ctgaagggag ttgtccgata ttgagacctt tggtacgata tttaccatta 60840 ctatatctga tcaaaaacgc ttggtcagag gccagcgtgt aatctgttac aacacctccg 60900 tcagcgtggg tggtgctaaa catccgggca ggggttgtga tactgctgcc agcagaggaa 60960 atgatatttc gggaatttga tccgcagccc cagaagtatc cgtctgtttt cacgaaaaca 61020 acagtgggtc cactggatga ccaagatacc acaccatctg aaaccttcac aaagcgtgtg 61080 gttaaggaag ttcccccgtt tccagcttgg cctttagagt tatttcccag ataccacagc 61140 gaaccatcct ttttgaggat atatacgctc tctgtgtcgc tgtaaatctc cttgatatta 61200 tccccagaca aatccccaat acttccaggc gctaccgtgt cgatttgcca agtatatgca 61260 gttgtactgg atgaccgaag atccggggtg taaatggaac cgtcgttctt aattgcccac 61320 agcttctcac ggttgcttgc aatccagttt acgtcctgca ttaccaaaac ccattggtta 61380 tttcggttag ttgtgtcccc aacaccaagc tcacccaccc ggttgaatcc tgtaacatac 61440 aggttcccct tatcagtcag gaccatactg tcgtaagacg taaccgaaaa ctgcttgatt 61500 ttttccttgc ctgaaagaac aaacggtgga ataatagcaa aaggaaacat aatacctcca 61560 aaataaaagc cgcccgaagg cggctgtatt aacctcggtt aagaatcacg tagtcgaaga 61620 ttgaaccttt accacggtag attacttgga ccagagagat tgcgtttggc gctgtagcca 61680 cttctccatc accaatcttg aggtaagctg ggtcaaaggt cacagtgtgg cccccaacag 61740 catcctgctc gatatagaag aagtaagtcc ccgcgcccgg caactgagca atggaaccga 61800 taacggtatc ggcggtaacc gtcacgttat aaacggactg aagcccgtct ggaatccaag 61860 accccgatcc tgaagtgata tcagtaccaa tgtcaacggt gaacaacttg gacttaacct 61920 ggcctgcatt gaaaacgctg tctcccgcaa aacttgtcgt cccggtgacc acaaggtcct 61980 tcgtcgttgt cgtgccggat gccgtgatat ccacggcccc gattccggct gacgccgcga 62040 cactccgtgg ggtaatatcc ttccctgtga agtccatact tccggtaatg gccccagtta 62100 cccgcaagtt tcctccaaca tcgaggtttt tatccacctt agcgctggaa agagactgca 62160 aaccgtactg ggtggtgggc tgggaagact ggccgaaaat tccattagcg ccctgaaccg 62220 tgcctgaccc tgtaatattc cttgcagaaa gatcctcggc gagcacaagg ctgttcatac 62280 ggatatcttg agccgtcagg tcaacaatcc cggtaatggt ccccgagacg ttcaggtccc 62340 cctggagatc tgaatccccc tgcacaacca agcgaggtgt atcttgacca gcaacgcgct 62400 tcaggagcag agtttcgaac tctgtggatt gccctgttag cttcgcattc acacgagttt 62460 ctgccaggtt tgtcaacccg gaaacaccca aggtccccgc agatacgtta ttggtgattg 62520 tcgcgctctg gcccaaaata tccccggttg attggagacg aggggcctgt acagagacgg 62580 tagccacaac ggagcgtggt tcaatgtcct tgcctttaat gctttcaacg gagccgatga 62640 cgttgccgtt ggcatccgtc aatgcaccat tgacctgtac atcgttgaac gttgccttcc 62700 cttggggtga catgatgtct ccaagagtta ctgtcagccc gttacggaac tcgacagggc 62760 cacggagggt agagccatca ctgatgtcca aggtttttcc gcgaataaac gtcgcagatt 62820 gaacactgtt cggaagaata tctttgcctg taacatcaaa ggtgttgaca ggattccccg 62880 cagcatccag aatatcaccc agaacttgga tgtcttgagt gatgatcttc tcgctgttgg 62940 taagattatc tgttgtcaca tccccaaatg cagaaggatc tacagcagca attgacttaa 63000 ctgtcacgtt cttttgagaa agatcaagtt caagcccggt aacaacccca gtgaaatcaa 63060 catctccgag ggtagtcttt ccagttacgt cgagggtggt tccgacttta acccccgctg 63120 tagtggtcac ggagcgaggc aaaatgtcgt caccattgac atcggctgtt acgccagttg 63180 tcgttcctgt gacaaccagg ttgactacag aggttgttcc tgtcgtctgg gaaatgttac 63240 cattaacaac cagactatct gcctcaactg cacctgtttc catgctggcg ggacggaact 63300 ctccagtcac cgtcaggtta cccccgatag aggcatttgc attggtagac aatgaacctg 63360 ctgacacgag gcccgttgct tgaacagaag aaggcttgat caaggttcca tcaacatcac 63420 ctttgacccc ggttgttttt ccggtaacca ccaagtcctt tattgtcgca gtttctgcaa 63480 cgttgatatt atcaacactc agatccgacc cggacaggtc gaggttagct gtcagggtgc 63540 caagcacttc aagatcttga gtcgtgatct ttccggttgc agtggtcgtc ccactgatct 63600 gcacgttgcc agtagcaacc aagttcccgg tgttcacaga gcggggcttg atgtctttac 63660 cgtcaacact gatagcaaca ccagacaggg ttccagtaac ctccaggtta ttcaccttgg 63720 ttgttccgcc gacagtcagc ttccctgtca gggaggtatc tttgccaacc gtcaaacctt 63780 cgtcgatgat aacactacga ggatgaatat ccaagccatc aacatcagct tttacaccct 63840 caatggtccc agtcacccgc agcgtattga tctctgcaat accatcaacg atcaactgag 63900 ggcgggttgt gctgtttcgg ttgttacctt ccagatgcag gtaatcccca cgcatgtacc 63960 catcgacatt cagggaatcc gtggcaacag caacatcacc aatctgggca gctttccctt 64020 tcaactggtc aatcgtcaag atgcctgttg acatcgtgta cggcttgatg ttagtctttg 64080 tgctcgcccc tgctggaggt gcggtgttct gctcaaaagc gatctcggta ttgtctgcgg 64140 tgccgaccac acgaatacga cggaaggttg cttcggcgct ggcagcggac aacttatcac 64200 tggtgacaat gaactcaccg ctgatatacg tgtctttgag aacggcatcg ttacggacgg 64260 tgatgttttc gaaatcaccc ttcatagagg caatgatctg gtcttccagg tcctgcatct 64320 ctgactgaac ccacccggtt gtcgggacgg tttggctgtt gtcgccaggg gccggggtag 64380 gcacacgggg gttaccgacg aagttcggag agttcagagg ggcatagata tcggcgatgt 64440 tggttttgtc caagaaaacc ttgaacttgc cgtcattgat cgggctgtca cgcatcacaa 64500 ccgggtttga taccgtagct ccgggcgcat accaccaagt ggtgttcccg cttacgtcgg 64560 tgaaggagta aagctggttg aactcgttga cagacccacc gtttctccag ggaagaggtg 64620 gggttttgaa atacaggttg tcgatcagcg ctttgcgaac agacgggagc aagctcccat 64680 cctctgcaac agcctgctcg gtgcccgtcc cgttgaccac gatgtgaaga cgtttggagt 64740 cttcgatgat ctggtcaact gccttgtcag catcgctgct caacagggca atgatatcag 64800 tattggatga cacgcgaatc tcctcttatt tggattcttc gttgttggaa acttccgact 64860 ctttcttgac aataccccaa cgaagattga tgaggtcact cagaaggtga ggtgctacta 64920 ctgtgggtgc ataaacccaa acataccact ccgggagggt gttgttgaag ccgtaccaga 64980 ggaaaactgc cgtcatagcc gccataccca cgtttgacca gaacttcgtg tgggaagcct 65040 tgtcaggttc gatgggggaa gttacgaggt ctttgaagaa ttttagcata gaacaccttc 65100 attatcaatg cgtcacacca gatgggcaac aataacaagt gccagaacgg ttcctgttct 65160 gagccactcg gagactgtat tgtccttcca ggaatagacg aagataaaca cctcgatgaa 65220 atacctgtgt gcatcctgta cggcgaagga ataaacccaa tcggggaata agattggata 65280 ttggattgcg aatacttgta ccagcatcag aggcagggcc agcgccaaga cagcgacctg 65340 acgataccct cggacaaaga gtgccacggc aaataaaagt attgaatcca atgcccaagc 65400 tgtggtaaag tacagcgtga tgtcgaagaa ctcggccccg ctgaaaaacc aaaacatcag 65460 gttttcgaga ataagatagg cgaggccaag ccccgccata atcttggccc cggcatccgg 65520 gcgagttgcc aggatgacga agcagagtcc aaaaataatg gacgtgatca tttgttgcga 65580 cgactctcca acatgacgtt aagtacggag cggatgtcct tctgaagagc gttagtctct 65640 tctttggcct ctttccgcat gtcagtgatg tcggcacgaa tgtcgccaat agtgtcggtg 65700 atatgcttga tcttacgatc caggatgtca tcaatactat ctttgctgac catctcacca 65760 cgaagccgaa tcagatcttt ttcaagagcg tcctgcttct cctgcatttt cttgaagtcg 65820 gaataaaaga ttttgagtac ccccaacaga aggaaagtca ccaaacccca aagagacttc 65880 agaatcaaca taatatcaag agccatgtgt gttctcctgg ggccgggcaa atacccggcc 65940 tgaaaggtta tgcagcggtg aactcagacc aatcaatttc actcgcggga gtgtcatcag 66000 agatccgatt tagcttaaca cgaagtttca gaagagcatt atatttctca atctcctctt 66060 ctgttgcttc acccagtccc actgcatcag ccaatggtgt gatctggctg gacagagacc 66120 ccatgatggc tttcttacgc ttggcagttc cgatagccaa gtcgaagtcc accttggtaa 66180 ttttcttccc atcgaacatc caaggcccct tcatgatgtc gaagtccttt gggatgctgt 66240 tcaggtcgga aatagacaag cccaacggat aggtcataga aatatccacg gacttgacca 66300 agaagcggac cattccgaac tcgtcgaaaa ggaccacata tttcttggta aacaggtcga 66360 gactctcgta aaaatcacga cccttgtcat cagaaaaata ctgaacacct tccccgaagg 66420 ctttcgcctc cgggttgtag tgtttgaagt tgttcatacg catgttaatt accccgtgat 66480 cgtgacccag acgccgttaa ctttcttctg gatcggctta taataaacac caccgatgtt 66540 gtcgccggag tttgacccgg tgtcttgtac gatgatacct gtcagagcac aaccttgagg 66600 ggcttggaaa gtccaactga tttcgttgcc gggcgggatg taagatccta ccgcaccgag 66660 acggttgtcc tgtacgctct cttcaacctg ctcagggata attgtcactg aacgtgtcgc 66720 agaaacacca gagagagtac cccgtaccgt gatggttgca ttcccagcag aaacacctgt 66780 tacgtaacct gcatcgttca cagtcgcaac accggagtta tttgacagga attgcagagg 66840 ttcgttcgca ccccctgggt ttttgttaac aacaagcatc ttcgactctg tgaccggaat 66900 ctggcctgga tcagcaatgg agatgctggt cagaacagag tagctcgtaa cacggatcgc 66960 tacgttcatc cccgtggaga tggaagcgat gatgttggtt tcacccggac ctacaaggcg 67020 gatgttgcca gagccatcaa ccgttgccac cgcagcattt tgagtagacc aggatacagg 67080 gtattgacct gcgatggaag cagggagtac actcgcagtc gctttgacgg tgtatcctac 67140 acggttggtg attgtgtctg gagagacgac gatcgccgtt ggtgagttgc ctccggagga 67200 ttgtgcctgg tcagtcttga taatgtacat aaccgcgacg ttcttcggac ggttttccgg 67260 ggccgttgga acttggcggg aagcatcgaa gctatactta cgcacttcac cactacgaga 67320 accttggtca ccagaggtca gtgcgccatc gtcacggaac acaccaccaa cgtaacgacc 67380 gatccagcca gactgaccga tgtccgcagg gaagtgaccc gtcatgtttc ggatcgcgtc 67440 accctgcaca gaaccaaggg cacggcctgc atctggatca ttagcgccag agccgtgtgc 67500 ccagccgcgc aggaagagac cacggtagtc aggaacaacg ttacggccca gaactccata 67560 cagcttaggg tttgtcccgg tattaaacac ctgaccgtta cactccaacc agccgttagg 67620 gactgtagac ccaggccaag ggataacggc accgattgga acaagacgtg gctcaaggtc 67680 atcaattcgt ccgttgactt gagcaatcgc agcatcaaag tcttgacgca gtttgttcag 67740 atcgtttcgg acaccttgga tctcttgctt cagttcctga accttctgat cgacgtatcg 67800 acgaagatct gcaacatctt gcttgagttg ggcaatatcc tgacggataa cgttcaactg 67860 ttggttgatt cggttttcca gttctgccaa gagccgggcg atctccgcct caagttccgg 67920 gaacttctcg ttgacgatcc agttcaccca agcagaggac atattcagga tatagttaaa 67980 ctcttctgct gctggctttt gtcctttatc ataacccttg gcgagcaagt cgtcaatagg 68040 ctttgatttg ttaggacgac ccgtgcctgg aaggtttacg tcggcagcag cccaaacgta 68100 aatagggtcc tttggtttag ccatgaaaat ctcctcacgt gaacgaccag tcttcctcgt 68160 cgtccgatac ataaattagg gtggacatgc cacctgcccc ggtacgctct gagtcataaa 68220 ccgatgcgaa cccaaatgcc tgtgcgtcac cattaaaacc gaatgggtaa ccttggttct 68280 caaccacacg gaggtgggtt accaagggga gcatgtcgat gatctctggg aggatgtcag 68340 tgacttccat acaggtattg aaaatgttaa tatcgagacg gtagttgtaa cctttatagg 68400 ttgtcactcc gtcttccccg aagagttgct tcagggtggc aatgatgtcg gcgcgggtcc 68460 cgctcttgga gttgttcccg gtcaagatca tgataacagc gcgatactct gggtcattca 68520 ggccgttacg gtaaatgccc aactgtctgc cgatttcgtc aaggatggcg tcttctgcgt 68580 tcaatgtggt acgcagttca gcaagttcaa ccatcttttg gtcgatatac tcaaggcgct 68640 ccaacaagat ccgaagggtc ttctcgaagt tctctttgcc tgtgaaaata tcggaaggga 68700 cgtaatcaaa acctcccttc acgaaatccg gcagagggtg gatgtggtta acatccttca 68760 gtacaatttc ctctgccatc ttagctcctt acacgatctg gctgaaaatt acgtcttccg 68820 gctccaagga gaatacctgc gtagtccctg cggtaacgtc agtgttaacg tatgctgaat 68880 ccggctcgcc cttgtttttg atgtccacat agacctgtgt gaaacggtcc ggagaggtag 68940 aactcatcac agtgcccacc aagcgaacgt tgtacagggt ccctgcaatc atcgtcccat 69000 taacagcggt tgccaagcca ttggcaatgg cagtctcttc tgtaacagac agtggacgtc 69060 cacggtatcg tacccgaata gccaactggc gagcagtagc cttcgtatgc caaatacgtt 69120 ccacttggtc atcttctgtg gtgatatcgt agaaggtgtc accatacgtg ttgttagagg 69180 cagcgatcgt gtcgtaaagt acctgactga tctcggcggt gcccccacca taacagacgg 69240 tcatgaaacg atacgggggg attccaaggc tgtttgtctt gtccgtgttg ttgttaaaga 69300 tacgaacctt ctcgatacct tccaccttgt tcagcaacgc tgcaaggatc gccgggcggg 69360 ttgctttgcc ctcgttgatg gaggtcgctg cgcggatgcg gtattcgtta tccgactcaa 69420 catcagagcc gtcgataaac gcagacaggt tatcaatatc aacaaatcca cccggggtcg 69480 ggttgatgct acggacagaa tggacatcac gggaaatgta gcccggctca attgagcgaa 69540 catccataga gatgctgcga gtgccagcca tcgggttagt acggaagtcc acgcgacttg 69600 acaagccgat catgttcttg ttagtatcat aaccgatgta cagcgcaccc tctgtggaat 69660 cgatccagat acgatcttgg ttggacagga tcgtgttgtt cacgataaag tccttgatct 69720 gcccgaagaa tgtcatcaag ggctgaccag aggtcgcggt caaattcagg gagagggttt 69780 ttacagactg gtcggttgtg ttctgaattt gcaggcggta agttccgact gacaaatcag 69840 tacccttaat aagctgggcc acaatattac ctgctacctg aacgtcagaa gacagttcat 69900 aatcggtgtc gattgtatag gtagcggcgg aataaatcat gttatacggg actgaggagt 69960 cgatcgtcat cacgactgtc ccggtactgc gggtctttcc gaggcggtag ataccgcgct 70020 tgcccaagag gtcatccaag taaatgcctt cggcaccctg catggtctgg gcgtagtaaa 70080 cagaacccat caggagccag agttgatact ctcgctcgtt aaaaatctga atcagcttgt 70140 cggcaacgga gttgctctcg gtgttgaaat cttcaccaaa ggcggacttg aaagtcttct 70200 tcgtttcctg aacaaggtcg tccatcggcg ggaggttaaa tccgctgcct gtcaaaccgt 70260 aattctccgc cattactcct cctgcgctta aattaggcga taagcgtatt ttatcatatt 70320 ccactgagaa ttaaaagaaa agggagccag aggctccctt tgtcatttcc aagtattaac 70380 ccacgtactg tcaccgtatt tcggcagttg gaagttaata aggtagtaca gtttgttttt 70440 gaaattgatg atgacgcctt cgtcaccaca aagttgcacg ttgctctccg gtggtgtagg 70500 atattggtac tggtcctcac caacgaacgc caagttgatc tcttcacctt cggcagtcac 70560 cacggtgaag aagcacttat acgaacgacc aaccacatcc atcgttgact ggaagtcggt 70620 gatttgaaga acatccggct cttgccgaac ctccgacttg atccttccgt cgaggactgc 70680 ctttaccgtt ttcttgccaa tgaaggtccg gtaagggaat ccgaatgttt cgtcaaagta 70740 ccaatcccct gcccaaattg cgaaacgcaa atacaggcgc tggcgcaggg acagttggtt 70800 cgtaagagcc agtctcatgc cccggtctcc cggggttccg aagtccagat ccccggtcaa 70860 gggatcgagg agcaggtctt taaactgagt agccatgatt ctccttagtt cggcggattg 70920 gtgttatcac cgccgcgctc tacgccgccg tgggtgtggg acatatactt ctgatagaag 70980 tcgtccatgt caacaccatt ggcagtaatg attcttccag aggtggtgat ccgtgctccg 71040 ttacacatca actgcccaga ggactgaagg gtgaatgatc cacctttgtt tccgcccttg 71100 atctccccgg aaggactcat ggtgaactcc ccggtgccat tactgatctt gacgttgccg 71160 tccgcaaggg cttccacagt aactttaggg ttctgaaggg aagtgtcccc gttaggcttc 71220 agggtgataa tggccttatc gttctccaag acaacgttgt ctggatgaat cgcctttgag 71280 ttgccatcgg tgaagatctg ggtgacggcc cacccggcaa acagttggtg agtgttctga 71340 tcgttattgt ccccctcgtt ccgctcagag aaggagagac ctaccacgtc gccgggctta 71400 atcggcatgg tcaggcgggc cttaccccct gcgcctgacg gcaacaggac ggggacatca 71460 taaataaccg ggtatcggtc tgtggttcca gaagggaact ctgtgtaggc catcggttga 71520 acatcgacag acggtccact gtagttcacc ccaaccacct tcccacgaat gttcgtgtgg 71580 acgttattac gcaggaatcg ggaaatgtaa attccgaatg ctgcatcata gcggtcagcg 71640 tatgccacgt caactcctta tttgatcaat tcgccacgtg tttccaccag gccaagttcg 71700 gtaatccaat ccccggtttc gtagccgcct ctgtgcgtca actcggcaac tttgtagaag 71760 cctttgtggt agcgagtgtt gagatagacc gtcgattcag gtaggatcgc accattcagg 71820 agggttgaga ctgtcatccc cacgtcttcc ttgatgtcgt gctctttttt cttcttcgat 71880 ggggctttgg gttttttacc ctctgcctcg tctgacttgg ccttagcttt agccttaatc 71940 agctttttag aggacgatgg ttgtttcggt gtcggtgtgc caaccatccc gccctcttcg 72000 gaaatctcaa acatcacgtt gttgaaacga gaaccttctt tcgtccaata cacagcgccg 72060 tcttgcacag agaacgtaga tcccgtattc ttagccaaat tggcaaggtt ctgggaagcg 72120 ttacctgtaa aggccataga ggtttgcaga gtctgattac caaagtcaat cacacgaccc 72180 tttggcagtt tcaaatcagc aatcaggtca ttgagtacgc tgttgatagg agtccctttg 72240 cggtaagacc gcgccgtggt cgcagttgtc aggttcaatg taccgtcgcc gagaatgaac 72300 tttgttgttc tcgtttcttc tgggaagtcg tcctctacgt actccactgt ccctgagaag 72360 atcagctttt cgtcaccgtt gtaacccgca tgaagcataa ccgcaagaga ttcacgctgg 72420 tttacagaga gatagttgac aacctcatct gacaagttgt agactgtgac gtatcctttg 72480 ttgggttcct tcgagttatc cttcttcaca ttaaattcaa tgtggaggtc tttaatctcg 72540 taggcatcac ctttactggt gttggcaaac ttctcgatgt taacgggctt ctcaccgata 72600 accaccggac gaccaatcac caaggtataa gtcctctcca tagctcgcat tattcaatct 72660 cctccacgtc ctcaataagg gacgaatagg tcatctggat acctttaagg ggtccgatgt 72720 taaaacgtcc ggcccgggtt ctgatgtccc caaggggcca gaggtaaagg ttgccattag 72780 gtacgttgtc aagatagcgg taaggggcca agatgtcaga gtagcaggtc agtttagagg 72840 taatggttgg gcgctgggca ccgacatccc ccaaagacat gaaccatgag tcatctcgtt 72900 cattccacat gaatcggagt tcgtaagtca cggagtccaa taccacacgg aatgtttcgt 72960 ccgggtagcc actcaactca aagtcaaagg tccgaaccac agtacgttct gtaatgttag 73020 ccaaaattac ttcctcatca ggtcagatgg gtcaaacggt agcttcgctc ccggcttgcc 73080 gccgaaatca gtagtgccga tagctgcgtt ttcccagcgc tcttttgttt cagcattctt 73140 cccgatatac ggtgtttgac gcttctgctt cgcactgtca tccaccgcgc cgccttcagc 73200 cgtttgcttc gtcccggtgt tggcgttttt agccttcttc gggtcagaag tacgagccat 73260 tacggttttg ttaaccatct tgaagcggaa ctcttccaac gtaacctgca cgttgattga 73320 agatcctgtc tctgaactac gatccacttg gaagttggtg ataacatagt tactaaggat 73380 attgtcctcg gtgacgagag ttacaagctg gtgtgagtcg gcaatcagtt cgaggatctc 73440 gatggccttc gccgggcggc gggcaagcat cgggttttcc ttatctgtgt cccgatcaat 73500 catgttccga gggtcaatca agtaagggga gtcagtaact gtgccagaga acgtaaactt 73560 gccatcctgc gttgttacgt ggtcagaggc ttttgccttt gactccaccg catagcttga 73620 cttgttgtag tcctttgtgt aactatggtt ggtaactgag tcgaagagga ttgccagatc 73680 atctgtctgg tttttgctct cgaactcgtt cagccgtcca cggcgcaggc cggaaacaaa 73740 catggtgtac tgaatgtctt cacgtgcctt ggcgttagaa accgcctttt ccttccgatc 73800 ggctccagcc cgccagtcgt caacggcctc cttcattttc ttctcttgtt ccgttgacgg 73860 aacgtaaaca ccgttttcgt aacttgccat aaaagtcctc ctgtgctagt attttagcat 73920 gaatgcaggc aataaaaaag ggggccgaag cccccttgat tagttaggcc caccctggat 73980 gagcatgttg aagttgaact ggttgttctc atcaacaacg gcacgaatca tgttcttcaa 74040 ttcgccagcc ttgatctcga tggtcacgtt gccctcaacc ttttgaggag gctgattgat 74100 tgggaacggc cccggatatg gcccagaagc gccagcggta accaaacgtt caggatcgat 74160 ggtcgtgctt ggagcttcct tcttggaacc aaacaaagag tagaacgctg tcttcatgaa 74220 gagacccatg tcccccgtga gtgcttgctc gataggggac tgggttgcac gggcaggtgc 74280 catgaactca tttacagcct gagctttggc gttaacttcc tcatcgttca gccagttcca 74340 tccaaggata cctgccacca taggccacgg cccaggaaca acaggcagct tgaatccacc 74400 acccccgcga ggagcacccc aaccatttcc acggcctccg cccccacgtc cgcctctacc 74460 acctcttcct ccacggcctc cgcccatatc gccacccatt gtaccacgga tctctttaag 74520 agcaccacgc agtccagcga ttttgctaag gataccgaag attttgtaga gactgccgac 74580 gaagatcgcc atgccgactg cataccctgc ccagttgaaa acatccttga ggagttcacc 74640 ttggtaaccg agtttctcag catagtagct gacgatacgg tccaacagaa cgaagtcatc 74700 gtggatctct gtaaccatgt cccagaaccc ttcgatcaca ttgccgaaga aggcaccgag 74760 gttggtcgcc agttcaccgt tactgtcaag gattgttgca agatcacgga acacacgcgt 74820 catttgctcg ccgaaaccac cttggaagat ggcgttctgg aagttaaccc acgtttgctg 74880 catctgttgc atcgccacac ggttgctctg caatgccttg tccaacgcgc cgccgttacg 74940 ggcagcttca gcgaagtatt tggctacgaa cggcagaact ttggcagctt tcagttcacc 75000 cttctgcatc atatccatga gtttttcaac gtcgatggtg gtatcgttaa acgcttcctg 75060 agatgccttc acgaatacct gcaatgaacc cgggatacct tctgccaact gacctttcaa 75120 ttcttcagcc atgatctggc ctttacccat catctgcccg atcgcggtga taccacgttg 75180 gaacttaact gggtcaacct gaagggcggt tgcgtactct gagaaacctg tgaacagttc 75240 atcgttctgg gccttgttca gaatgccagc ggagttgacg gacatctgca cgtaaccctg 75300 agaggcaatc ttcagatcca gaccgagacg gtaagcctga tccttcagga actgaactcg 75360 ttttgccgct tcttcagatg aatcagaaac catcgtcata gttgcatcca tgccttcaaa 75420 gaactggccc tgacggataa tgcttgcacc agatgcgaac acactgtatg ctgcccctgc 75480 tcccaaaagt tgagaccgga tatcaccgag tgctgcacca aacccacctg cgttagcctg 75540 tgcggaacgg aactgacgtt cgagtgcctg gatgctctgg cgatactgcc cggcggacag 75600 cgccccggta cggaactgac ggttcagatc ttggatacct tgaccagcgg catttccacc 75660 gagacggccc gcgtagttgc gaccatactt ggatgacaaa cgaatacgct gttgtgccat 75720 aacatcttcg atgcgggctg cacgagccgc ttcagccgcc ttgcggcgtg tctctgcctt 75780 ggttctgcgg tcttgggcct gagactgact gcggattcga cctgccatcg cacggttctg 75840 ggcagcgatc aattccgggt tgtaaacttt gttcggatca ccaaccattc ccgttccggt 75900 tccaccacga ggtggcaact gtagggagtt gattcgagtg acgttcatag gacgagaagc 75960 tgcattcatt ccagtggtag ctttgcggtt agccttcttg gcttctcgct cttgctgctg 76020 gtagaatttc ttcatctcgc tcatcccggc ccgaggagca gtcatctgct gtacctgctg 76080 tttgatacgg gcagcggaca cctttgcgat cgcctctttg gctttagcct ctcgacgggc 76140 cagcgcgatg ctgcgttcag aggcgcgttt ctcttcacga gcgcgggttt ctgccaaacg 76200 cttattcacc aggcgcatct gctgggctga tttgtcgtag gcttttgccg ggtctttctt 76260 tggtttagag attgcatcag atgccttctc ccactctttg ccaacctgac ggatctgttt 76320 gaccgcccgg gcatacgagg atttgtccac ctcaaacgtt gtacggttga ctgttttggt 76380 gacaatcacg tttccacttg ccattgatcc tcctccggca attataacgc ctcaaaaaag 76440 aaaaggccag agtctttcga ctctagccct tatcctccct taaacttctt catgcgggca 76500 gcttgttcag cattcttctg tgcctgttcg atgtccttct gcttggcttc ctgtacgaaa 76560 tcctggatat ccaagtattc gtttttcttc aggagatctt ccatatcgcc agcttcaatc 76620 gagatctcat tgacaccctt gaaccgctcc attaccctga agtaaaggta gtcataccat 76680 tttagagaag agttcttccg tgcatactca agtgctttct ctacggtact tagcgggatc 76740 aggtttccgt ctgggtcgga ttctcgtcca gttgttccac cgctcgcgcc agccccagac 76800 gtttgaggaa cgtgtcgatg gtcccgaaac cgctcttgcc gaaaaaacag ccatagttga 76860 cttcaactac cttaccgagc agagtaatca ggtcttcaac gtggtcttcg aaaacttcgt 76920 cgatgttgat tggaccaccc atgctgtcta cttctacacc ctcaagcagc atgttgatga 76980 tctcttcacc accgtcctcg atctggtcga ggatgtaaag gatcgcagta gggatggcgt 77040 cactgaatcc ttctcctttt gagagggcag agccagccac cgtgcccatc gggaccgcca 77100 ccaggcgacc aatcttaggc atattacgaa tcacttggct tggagaccag tgataaattg 77160 tgaaacgctt accgccgcac agaacaactt ctgtggactt cttaacctta aacatgatac 77220 actcctgtgt cttctgttga ataatccatg ccgtgagaaa aggccacatc catgtggctc 77280 acgttgttat tggtccagtc caaccagacc agtgatcccg gcagcgacgc cttgaatctg 77340 gtcaggagac agccatgcgt ccagtacacc gatttcccag tccatctgac caacttcgga 77400 gccgtagctc aggtcaggct gcttctgaat ccagccgatg gtgttcaggg acagaccctg 77460 agaaccttcc acttggacag gcagataaat caggccagtt acgttagcct ggcgctgcca 77520 ctgggccaga tagccgttcc attgggccgt gttctggagg gagagggtca tgaccccggt 77580 gttatcacgg gacagtgcgt tactgatctc gccgtcaaca cccgcatgaa cggtgatgtt 77640 gtcgttgtta cgagtaataa cgatcttcgt atccgctgca aatccagaga cacgctgtcc 77700 accaataaag acgaggactt tcgcagggtc atacgcctgc atcgtcaaga ttttggattg 77760 gttcagcatc tattacactc ctacagcaga ggaagacatc ggggcctgag cattagtgct 77820 ctggcccgcc ggacgatcca gtacaacgga ggcgcggatc ttaacgtagt gcagagagtt 77880 gttgtacaca agctctacct tcacgtcacg caggatacgt gcagcaaggt cattcgtcgg 77940 gatctgggcg cgtttcggaa tagtgatcgt cgggtcgtag gacactttgt tttcagcgtc 78000 gtagccagtc agaataccac cattacgaat accgatgttg atcgggttgt tcatcagaac 78060 agacttcagt acaggcaggt cttcgtcgct catcttcatg gacagaccca tgtcagaacg 78120 gcgcttcatg tacgcgaaca cggattcttc catacggaat ttaacccaat gggagaagcg 78180 aatcacgtca acgtactgcc cagaagaagt tttaccttcg atgtagctac caccgccgta 78240 cagggaacgg tagatgtttg cgttacgaga ccaagcgttg gaacgctgag tgtcggagcc 78300 agcaaacgga accatacccg gcatcgtttt caggtgcagg gagtcgccgt aagaaggatc 78360 gttcgcagcc atcgcaccga tgacagagcc ttcggtgaag tcaacgtcag cacctgcgtc 78420 ggaggttccc agagagtcat aactcagagc cttcaacgtg tcgaacacgg aggcggctga 78480 gttctcaggg gcgtatgcgt ccacatcggt ggagttgtaa acgtgcagtt tgtagttcgc 78540 tttggagaac tctgcggcag caacgatgtc tgcatcagaa cgggcttccg ttgacaggaa 78600 gtaccagttg tggttctctg caataacgaa cggcagtgcg gttgcaacag tctcgccaga 78660 ttcgtttacg atcttgtaag tgcctgcgcc tttacccacg ctgaccacgt tcggcgatac 78720 cgctgcgatg gttacgtaag taccattcgc aatagcagtg actttgccag tcaaatctgc 78780 atctgcggta actgcatcag ccagtgccgt agcaatctgg gctggagtgt ttccaggcag 78840 tacgttaatt ttaacagcct tgaccatctt gttcaaggta atattaacga caacctgttc 78900 ggtgtttgcg gtcccggtga agtccacacg tgtagattcc agagacatac gtccgattgc 78960 cacacgctgc ggggcgaagt tgcctgcgaa tgcgttggtg gcaaactgat atgccgggga 79020 acctactgca aatccatcag caaccatccc agcagtggac acgtagaaac gaatacgctc 79080 tgggaagtta ccgtgcattg ccaggaacat cggggtttca aagccaacgg tgtcgattgg 79140 ttgagtgcca aggctaacct caacgtcaac aaccttgtct tgatattcag ccattttgtg 79200 tcctctgctt ctgaacgatt attgaccagc acaaatgtgc caatatgtgg gggattactc 79260 ccccgtcttg tcactgataa gcgctggctt atcaataacc ggagttgcca tacaaacctg 79320 agcgatggcg tcatggtaga cgatcggagg gttaggcttt ggtggcaggc caggatcatc 79380 accgccggga ttgacaccct tgtcatagtc tgcttcaata ccaacaggcg gaccagaagg 79440 gttgtcaata tcgatcccga taatgatgtg ctcgacatcc tcgaacgccc caatatcttt 79500 ctctacgaat gtcgcgttga aagtgacaat ggttcgtgct cggttctcaa acatttgctg 79560 gttcagcggt acacgcattc gagcaatgtt cgaggttgaa gagtacgcat aaggagaccc 79620 cgttgggaag tatttctcac gaatgaacgg caggccgaaa gcctgatgca cccgggacaa 79680 tgcccagtga ggtttcccac ggtaagcggt gagggtatag ctggcggtat agttgtgtgc 79740 cgtatgaacg acaccttctt catccaccac ttcgtttgtt gcccagtcga gaggggtaag 79800 cagagtaagg tctacgagga tgtatggccc ttccggtttt ggaatggtct catcagcttc 79860 aacgacatca taacccgtta cgtccaaaca gagacgaccg acagccttgg tcagtgctcc 79920 catcacgaca tctgagtttg taaaatcaag agggagggcc attagagtcc tccctcagtc 79980 cctacaggga cagcgatcag gtagtagcgg tagcgaggca caccagaaga gggatacatg 80040 tccgccttca tcactgtgaa ccatgtctga ccgccgccgg aatccggcag caacatctga 80100 tcggaaaggt gttctgtgcc ctctcgcgct cggaacagca gagtttcaga gtaaacggtg 80160 taggagtcat aatcctcctc gccgccgatc atcaactggt tgttctggtc acggatggct 80220 ttcccacgca tcggttgaat agtgcattcc acggcagtaa acggggtgta caacacttcc 80280 cgcccgccgt tcagaaacgg actatcaggg tccgtcttga cccactcacg cttccgacat 80340 tgcacttcga cacgagggat gagtctgttt ctgcttgaga gcaacgaata agcccgtgcc 80400 atcggtcacc ccttatttcc cttggtactt gacgattttg tacgttgccg cactcgacaa 80460 gtcaccgtaa tgaatcattg cgtcatcaaa ccccttgtaa ctcgcccagt cttttgatac 80520 ctttggatta ctgaatgaac ccgtggagat cgtgaagcga atactgtcgg ctgcatcctt 80580 cccgattttg gtcaggtagc ttgagaagcc ccggccctga atgatcccgt tgtgcatacg 80640 tttaatatgt ttccggagac cttcttggaa gtgcatggag gttgagaaca tgaagttgcg 80700 ctcaggaagg ttattccagc cttcttcatg gatggcggca agagtcgcca tattcagccc 80760 agaatagtgt ttctcgtcgt aaaaaccgta ctccacttct ttctcagcta aatcagagag 80820 ttggctgaaa taccgcttca gttcagagat gtcagtatat gacttctttg ccacggtatt 80880 tccccatgaa agttatttta ccacaatagg tcagcaaaag caacaaagtc aacgctttag 80940 cggaccatcc acggactgcg ccgaacacgg tcacaaggcc acgcggattg cttcgggatc 81000 gccggggtag ggtagacgcc ttgctccatg aagccgttgt aggagttagg gttattcttc 81060 acacggaaga actcatcttt acgaacacca ccgatgacga caagggagga gttgaagttc 81120 agagatgggt caatgtaatc agggttcagt tccagccaat caaggaagtc tttccaggat 81180 tgccagcttg atccgccctt gatggagata gtctcatcac cgatcttttc aaatctctca 81240 cgaattgatg aatttccgct ggagacttct tgtacgatca accaacgcac acagtcaaca 81300 acggtgttgt acagggccac cggaattttc tccgggtggt tatccaaatc atacgtcagc 81360 ttccacttgg tccagaagaa gatgatcgtc tggtccgaaa tttcctcagt ggtgatccca 81420 ccaaggagga cacggacaag ctcaattact tgttcgttgg tcataatagt ctccatgctt 81480 tagccagtgc aaccatttta tcatgttggt cacaaaaagt cattgacagg taaaaagaaa 81540 gccgcccgga ggcggctttt aattatggga tgggaatgat cacaagccag ccattatggg 81600 ttgtagacat tacctgtgag taactcactg tccccggaga tctgtactgc atgtacagcc 81660 aaacgcccac ttgtgtcgcc cacagcagtt ttttgggcaa cccctgccac tgtcacttcc 81720 catgcttcaa gggtgtcgtg gcaaaaaaca acaaggacta ccttgtcgcc gaaccccacg 81780 ttgatagtag agtccgacgg ataaactact gtatttgaga tcgaggtggg gccggttctc 81840 ccggtcttaa tacgatttac gtccgtgtag gttgttgtta gtgcataaga gaatccgatc 81900 ctcttcgcca ctgacgaagt tcctgcggag aaaccaactc gggtctgaga gacattgttg 81960 tttgcagaga gaataagacg aacattcccc aagttgttac cggggagttg aatccaagag 82020 ttcgttttgt tgatcgggga ggcataaatc ccagaaatgg tcgggagggc attgtcaccc 82080 ttatctccct tagggccagt ctcccccgga ggtccctgtt ctccttgtgg acccgcaggg 82140 cctgccggac ctactggtcc ttgatctccc ttttcccctt tttcaccctg aacaccttga 82200 atacctcgct gaccttgtgg tccttgtagc ccttgaggtc cttgctctcc ttgtggtccg 82260 gcgggtccgg caggacctgt cgcaccggaa ggccctgcgg gtcctactgg cccttgcaaa 82320 ccctgcgggc ctgttggtcc tgctggacct actggtcccg gcgcaccttc tgaggccatt 82380 agtgcccagt ggtcgttaac ccttgtacca gtagattccg gcgggatacc tgtggatggc 82440 tgagtagaaa aatatgtggc accctgatac ccaactgcat cgttaacgtc gtaggagact 82500 gaatcatcat agtttcctct ccatacaaga cctgtcgcac cggaaggtcc aacggggcct 82560 gttgccccgg cgggtccctg ttccccttgc gggccagcag ggccttcagg accttgaggg 82620 cctggttcac ccctctcccc tttctcgccc tgagggcctg ttggtcctac tggcccctga 82680 ggtccttgag gaccaatttc ccctgcctcg ccctgagggc cttgttcccc ttgaggtcct 82740 ttttcaccag gattgcccgg ctctccttgg acaccttgag gtccttgagg accggggtct 82800 cccttatccc ccttctcccc ttgaggaccc tgtgggccgg gtacagaaga tcccccctcg 82860 ccaatttggt caaaggggat aagtttttcg ttaacgagat cgtaaagtgc atacccgaag 82920 tatacaccgt tctgctcccc tgtgataccc ctcattgttc ccatacagtc tccttatgta 82980 caaaaaccgc ccctaagggc ggattaatta tgcggctgga aggtcgaaag atgcagtctt 83040 cgttgtagag tcagacatgg tgaaggttag agttacagta tcgcctgagc gagccgctgc 83100 gatgtcagcg attccaacac cgtctgcacc tgctgccccc gccgggcctg ctggacctgc 83160 tggtccagtg tctcccgtat cacctttgtc acctttcggg ccgggagtac cgccaccacc 83220 tgcaaaaagt tcagagatgg tcttgctctc tcctgttgct tggtccatga tctggtaacc 83280 agtcaggaca ccaagggatg ctccgccaat tgctgccatg ttattctcct acgaaagttt 83340 gaggaacttc agggatgtct gtgatgactt cggtgtcata cacagttttt acgtatgcca 83400 acaggttctg gtggaccttg gcagttgcag ccaggatttc atcaatcttg acaacagcat 83460 ccgcagacgg tagagtcaaa actgtgtaat cagctttgcg aatcagaaca tccgattcag 83520 agtttgcaaa cgcaaacgct ttggacagtt caaggttcag gagttctcgt ggttcaacag 83580 agaaaacgtc gccgtcaaca gccacacccg actgaaggtc agaggtatat tgctgatacg 83640 cacgggtacg ataatcttcc ttggcgcggg ccaagttctc tgcgatctct tctggggaga 83700 aatcacgagc aacccaagtc ttgtaccaca gatcatcttc ttcgtttagt tccggttttc 83760 cttcggtgac cacatcacca ataggaactt cagaatcatg aaccatgcaa taaccccaca 83820 ggtctcgcat gtccttttcc caaacgtaag aaccaatact tacgttagta tgttctttga 83880 ggaagtcgct aagatagaca ggatatttca tgtcggacaa acgaatcatc ggtgtccaag 83940 cataaacctt gctggtagga tctacagcca tttgaatctc cttgtgaagg gggccgaagc 84000 ccccttgggt attagtctac agtcattgcc tgaacaccag tcaggtccgt gccgtcccag 84060 aagaacgtca ggatggtacg ggtagtacca agtgctggct gggtgccgtt agaccatgcc 84120 atcggagaag gccaagtcat ggacccacct ttaccgagga acttaacaac cacagtcatc 84180 gcacgacccg ctggcaggtt ggagatggtg attgccttag acgtggttcc gtcaacggtg 84240 aacacgttgg atacagaagc atccagtgcg ccagttgcgg acatgacacg caggtcgtag 84300 cgatccagtt tctgccacgc accgttcgca cggacgtagt agccgttgtc ttgaggcgct 84360 tccagaactt cgaccagttc ccaatctccg tcgatacgag cgtactgctt gccgtctttc 84420 ggggaatcgt aaacgttacc gccacccatg taccccatcg gtgcccaagt ggttgcgttc 84480 gtcttgcgga agaactgcaa cgtcgaggag ttcaggaagt agtcacctac acgaccatca 84540 gcaggacccg ggtcatggtc gaacacgatc cacaaggaac cttgatcacc cttatcaccc 84600 ttgacaccct gcggaccagt agggcctggg tcaccagtag gaccagtagg accaaccggg 84660 ccttggatac gtccgaggtt ttcgtaggca gagccagtcc atccccagaa gtcgccgtca 84720 atcaggtaac cctgacccag ttgcccggtt gaaggcagtt ctgaagtgct gttgagttta 84780 cccatgatct caacgcccgg gccgatgtca ccttgcgggc cttgagggcc tgtagcacca 84840 gtgtcgcctt tcacaccttg tgggccttgg tctccggtat cacctttgtc gccctgcggt 84900 ccttgcggtc cacgcacaac tcccaagtca acccaggttg ttccgttcca agagtatccg 84960 gtcccagtat cctcagtgac gtagtaatca ccgacggtgt tcccggtagg aggcagggtc 85020 gcagcagagg ttacagttcc cttcggaata acacctgcgc ctgtggcacc agtatccccc 85080 ggatcacctt tatccccttt gtcgcccttg tcgcctttct cgccttgcgg gccgcgagtc 85140 aggccagtaa caacccactg caacccatcc caagcgtaac cattgccatc ttcctctaca 85200 gcccagaaat cgcctttggt gttgtcttgg tcaggaagat ctgccactgt aggaacagaa 85260 cctttcggcg tcagaccagt accctgcgga ccttctgggc cagccgggcc gacatcacct 85320 tggtcaccct tgtcgccttt ctcgccttgg tcacccttgt cacccttagc tccagccggg 85380 ccagtggctc cggtgtcacc tttcggacca cggacagggc ctgcattctc ccaagcagtg 85440 ccgttgaaga tgtacaggtc ttctccgatc agccatgcgt caccagttac cggattggaa 85500 ggcagttctg aaggatcagc ttttgatccg aggatcgcga gaccttcccc tacttcccct 85560 ttcgcaccct gcgggccagt aagaccacgt ggaccagtcg ggcctgtgtc gcccttcgca 85620 cctttgagag atgccaggta gtcgctcagg gaaccttgga acccgttctc taccgccaat 85680 gcgtagttgt ctttgcctac gtcacctgcg tctcccttgt cgccttttgg gccagcaggg 85740 cctacatccc cctgatcacc tttagggcct tggaaggcac cgaggttttc ccagttgtca 85800 ttcccagtgg atacccaaac gttagttccg atcaggtagg catcgcctgc atccgcgcca 85860 gccgggaggc tcgcttcgtc tgcgacttta cccaagaggt tgatggcgtt ggcgttagta 85920 ccgtcagcac ccgtatcacc tttgtcgcct ttatcacctt tcggtccttg tgggccaatc 85980 agggagttca ggaagtcaac ctgagatccg gtgtgaccgt tagccagcca tacctgatac 86040 gcagatttac cctctgggcc aatctgacct acagtctgcc atccatcttc atcacctgtc 86100 cacacataca tcatgtcttg gatgatgtag cagtcaccag gcttgttacc ttcatcaggc 86160 agataggacg ggtcagggaa ggaaccaaga atttgcagac cgataccgtc agcccctttc 86220 agggattcca gccactcatc aacagtacca acgaagcccg cgccttctgc aacttcataa 86280 gcagacagac cttcaggccc cgcgaagtta ccgatgtcct tccactgctt cacgttgttt 86340 tcgttgacaa cggtccagaa attctgtcca ataacgtaag tgtcacccag ttccatttcc 86400 gcaacagccg gaagctgaga aacttcgtca agctgatcga tgacgttgat accaatacca 86460 gccgggcctt tcggaccttc tgggccacga gcaccctgtg cccctgccgg gccagttgct 86520 cccgctggtc ctacgtcccc ggtgtcacct ttatcgccct tgtcgccctt atcgccctgc 86580 ggaccttgag gacccgggtc tccctgtggg ccttgagggc ctgccgggcc agttgccccg 86640 gtatcccctt tatcaccttt tacagtgtca acgtaaatcc cgtcagactt gacttgaatc 86700 ttgttgtttg ggtcttggga gattttagtc tcttgtgggc gaacgtacag agcaccctgg 86760 cggatttcca gcgtgttgtc ttgagccaca gaaacaggaa cgctcaggcc agcagtgcct 86820 accttcaggg cgttcatgga atctggatca acgatcacgt cgatttgggt cttggctttc 86880 ccggtaggat cagccttgat ttccagagtc ttcttcggtg acgtggttga cacgttcacc 86940 ggatcagagt atacaccttc cagggccggg ttgccagcgt tagcagcttc ttgggcggag 87000 atgtaagtta cggtgttccc atccttagca gacaccttgg tgtcggagcc agcaacatac 87060 agaccctgct cactggaggt gctcaccact tggaggcggt ttcctgaacg acgggagatg 87120 ttgatgtttg cttgcagagg ggaggattga gtaccatcac caatcagcgt ggtggatacg 87180 gtgtcttggg tttcgatgcc cgtgaaaggc gcaccagaac caccagaggt gctcgcagac 87240 ggttccggga gagtcaccag aacttccacg accttatctt catattgtgc cattacttgc 87300 ttctccggag gcgggattgg aggaggatgg ccccgcacgg gggccggagg gaattacagt 87360 gcagctttca ggtcttccaa cattgcgtca aaagtcttag ccttgctcag agagatctcc 87420 atagtcaaag cgtaggattc cagcgcagcc ttagcagccg cttttttggt gtcgtcacgc 87480 agggtttctg catgagcgag gatctctgcc ttttgttcgt cagtgcgggc agaagcgggt 87540 ttttcttctt ccttcacttc ttccttgact tccggcgcag gatcaattac agttgtttgt 87600 accagagagg acagtggtga ttccacctcg gtctcaacat cttctgggat cacgttggcg 87660 cgagccgcct tgatctcacc gatgttagcc aagaacttct ctggagactt aggggtctcg 87720 tcgaagaaga cgaggaatgc agcgcctttc tgaacagatg cgatcgtatt gatgcccagt 87780 gcggtcatcg cttcaatctg gttcaggaac tcataaaggt ttcggcccat caccagttcg 87840 ttgtcatagg aatacaggaa cggtgccagg cgggcagatg cgtcaacgtc cagtttcggc 87900 aggtattgac cgaagaactt gagcatgtgg tattcgctct ggaacacttc cagatgaggt 87960 tgtgccatta taggatactc cttcgttgat aatcaaaagc ctatcttaca atggtacact 88020 attttgctta aaatgtacag ttataccgca gtcaataaaa aaggccaccc gagggtgacc 88080 ttttccaatt atgcagtgaa cttgcctgtg gacttaacga ccagttccgg acggttgttc 88140 acagtcagga aggaagtctc agactcaatc ttcgcttcac gcaggtagtt ggactctttg 88200 aagaacacgt acagttcttg agccacctgg ttagcgtcac gcagggtgtc gctcggcgcg 88260 tagtagatct cgaacatacg agagatacca cgtgggaaga tgtacgcttc acccgccggg 88320 atgttcccgg aaatgtcttc gatgaacagg atgttcttgt gctcgaagat acggttgtta 88380 gcgttaccgc ccagacgacg acgcagcatt tcctgagttg acgggtactg agagtacgca 88440 ccagtcacct gcgggtgagc gatcagcgcg gagaaccact gacgggacgc cagaacgacg 88500 atctcgtagt tgtcaccgtt gtcgccagcc cagtcgatga tgtgagcacg agcttcagct 88560 tccaggactt caattgggtc tacgcccagc ttggtgaagt caacgtcagc agtctgctga 88620 gttgcgcccc aaacttcgta gtagttgtag tcagaaaccg ggtcatccgg agaccaagag 88680 gtgcccttca gggcagcata catggccttg gacttcagga tcgcgtggct acgacgaata 88740 cgtgccatgt gacgctgaac ttccgcatca acagttgcag gggcattctc agtcccgaac 88800 tcacggaagt tctgaatgtc tgcacggtcg atggaacggt ccagagggaa gaacgggatg 88860 ttcaggttga cgatcttttt acgatcgtta gccaggtgct gacgcacacc gccacgctga 88920 acagccttga tgtcaccagc gccgtcatcc agacgttcga tacgggcaac agtggtacga 88980 ccgaaatgtt cggtgaacag gttcatgctc tgaagcagag tgtccacacg tggcatggta 89040 gagatcaggc cagtgtagtc gagaatctgg aaatcgccgt tttgcattta aatatctcct 89100 gttaaattac agtacggaac cgatgaactg ggtgccgaca atcttatcag ttacctggtt 89160 accttgggct tccagagctt taatgccagc agcatcaatg tcagagccgt cagcatactt 89220 cagtttcaac tggttcaggg tcaggccgcg aacagcgaga accataggga ctttttcgcc 89280 cggttcgaaa acgccgatgt agtggtcaac gcccggcaga accttacggt caaccagaac 89340 gccgaagacg tcagcagccg cagtagcagg ggtgccatct tcgccaacca gcataccagc 89400 gaacagagca gttttggaat ctgcatccgc atcagatgcg atgaaggtca tgtccacttc 89460 tttgaagttc caaccagtat cgccgaaagc gacaacacgg cccagaacga tgtcagagta 89520 gttaccttcg agagtagcaa cgtgtgcttt agccatttgt caggttctcc agattaggcg 89580 ttaataaatt cagcggattt agcggctttc gccagttccg cagcgcgttc tttgatcaca 89640 cggtccaggc gggcctgtgc atcttcggaa cctttggcta cttcagtcag agtgccatca 89700 gcaccgactt ctttgccatt tgcgaaggat tttttaactt cttcgatctc cagatcttta 89760 gcagccagag cttcgtcttt cgctttcaga tcagcagcag ctttttccag tacggatacc 89820 agaacagcct gcgccgggtt ttcgataacg tcagcaacaa catcgcgctg gtcagcagct 89880 acgaagctaa agccagcggc tttctcaacg gcgattgcac gtttagcagc agcttgggac 89940 ttgacgatct cttgggcagc ggtaagagtg ccttccatag atttcatctg cgccaccaga 90000 gcttcgttgg ccttcatcag gtcaatgaac tgctgggatt tggttacatc aacttctttc 90060 tccacgggaa tctcctcgtc gggtttgata gtcgctccag cggagctttc agttggcgct 90120 acaggagtca cagacttttg cgtttccaca acaggcttga cttcagcggc gggtttgact 90180 acagttgaat catcaccgaa gtcgatgccg aggtcacggc aaccttcttc gaagtcctta 90240 cgggccttaa tgagttcaaa ctgttcaagc agtgacagtt catcgccagc cttcgctttc 90300 ttgataaggg tgacagagga gagcttctgt tccaagtatt cttggtggcg ctcattccat 90360 tctgcttccc aggaatcatc atccggcggg ttgtcttcga tggactgttc gaactcggtc 90420 ttcatgccca gcactttggt gagcaactct gcatctccac tccataggtg gaagaagcgg 90480 cgcaggtact cttccataga aatggtgagt tggacctgtt cgagagattt gatgatgtca 90540 accgtgagtt tctcagggtc aacttcggat ttggtcagga ggctgacgtg gcggttattc 90600 gcgctgtagc cctgatctcg gtgagtgaga gcaatgccgc gagatttaat ctggcgacct 90660 ttctcgatcc acgtcttgat catttggctt cctcttcttc gagtgaaata ctgaagtcca 90720 gttcggaaag ttcattcgtc tcttcgttca gcattccgcc gcactgaagt gacaggccac 90780 cgataagtcc ggccttcttc aactcccatg tctcaggggt gtactgaact tttgcaaccc 90840 aagatccggc cttgattact tcgccagttt gcgctacatt aacgtcgaac tctggttgaa 90900 tccagtggtc aacgattttc caagagtcgg tgtcgaaaag gtgatacagg ttctccgtga 90960 cagcaccgag ttcctgagcc tttttgaaat cttgttgagc ctttgccaag gtctctttcg 91020 acatccactg tccgtgaagg tccggggtgt caggttcgta gatgatctca taagaaatca 91080 tctcttcttc accagcggct ttctggacga ggacggagcc aacggccccg ttaacggcag 91140 cagctttatt aaagccctgc ttcaacaggt cgttggcctt tttcacaaac cgactctttt 91200 ggattcgagt cagattagtt tgtgaaggaa ggtcggaaag ggtaatgttt tccatcattg 91260 ctcccgtgat aattatttta gcactgaact gactgttttg caacaggttg ttttcacgac 91320 tggattaatt atccaaatta gccgctgaat tatctcttga agcagagatt ttgccagtac 91380 cgttaccaga agatcctttc gtcatgccgt ctccagcgcg actggtatcc tcacccaaca 91440 gcttcatgag ttcttcctga gaaatgtctt cctcaatctt ctcatcaata cccagaactt 91500 ccagtacacg gttgataacc ttcggagttt tcggcaggta gccaacggca ccaatacgct 91560 gaatcgcctt ggagaaacct tccatatcca cgtccacgat ttccccagcc ttgacgtatg 91620 gcatctccgt ctccgggagg cgcatatcgt tcagggccag caattgtggc agcaggtttt 91680 cgttcagagc ttccagaatg atctcgttta cacgttgaac aaactgggtg tggatagtct 91740 gcttggactc agacaggttg taagaaccgc ctttgtcgtt acccacgttg atgaagccag 91800 cgcccagacg atcaaggatt gacttcttac ggtcactgat caactgtgcg gtgctgtact 91860 gcttacccat cccgtcgatg cccttgaggg tcattgaata ctgaggggcg ttatcctttg 91920 tatcggacgg cagcatgaag aatgactgct caccggagtg ggcatttgca gcgtcagaca 91980 tcaggccacg gaccatttct gcttcaggag aagactggtc cattgcagct ttgttcagga 92040 tctgggaagg gatcttcaat tcgatcacac cacccatgtc cttggttgcg ccaacaacct 92100 caaggttctc aatcagaatc ttttcacgcc acgctcggta gcaacctacc agaggagaga 92160 caccgagagc ctgggatgaa gagcctcccg tgttcatcag catgacacga ttgattggga 92220 taatgacatc actcatgcgg gtggatacgc caccaacacc aatctcgctg gctgtgtagt 92280 tctggaacgc agatagggat tgtttaatac cgatgatggc agagccatcc tcattgtaaa 92340 caatcgggtt tgtccggctc aaggacgcct gcggtcggaa agcaaggttc tttaccttgt 92400 acttgccgat gtattcccca acagtaacct gagtgtagac tttctccaca actgacaggc 92460 catactcgtt gaatgttgcg gcgtcacggg cgaactgacg aagggtttga ccttccatgt 92520 tcttcaggca atactcaaca aacttagcag cctttttgga ctcctcagat tcgccaggga 92580 ttaccttgaa atccttgaac gccttctcca cgaaagtgta cagcatgtca aggcccgtcg 92640 caacggtggc gtcctgcttc attgactcga ctgtaccgat caggttgggc cagcgaatct 92700 cttcggcctt catacgttcg gagtcaacct gaaccttagc cagagcaaag gtcccttgct 92760 cactcatacg catacgggat acgttaattt gctcgctggt ggccttagcc actttaacgg 92820 agcggttacg attacgtttt cttgacacgg catatcccct gtgatattaa taacgacgtg 92880 aggcgtacag agtaggggca tttggtcgag ggatagcgac ggcgcggacg acctcggtct 92940 tctctaggaa attaattcct gatgccacgg catccgccca gtcatcctta cgggacccgg 93000 tagaacgttc accgttgaaa gactcaagtt ctttcatcaa tgcgttgtac gtgtccagag 93060 ggaatgactt cttaacgatc cggacaaatc cgttttgtgc caactgggca aacggggaga 93120 agcgggttag cttcgatttg ttaccgggtg tcgggtcttt ctctaccata taccctttcg 93180 agataagatc acgggatgac gtggtgaact cggagatacc tgcctgaccg gggtcaacgg 93240 agaaaatgac gatcgtgtcg tcaccgtcca gcattgcctg cttcgtgata atcacgtcac 93300 gttcgccagc tttcttacag aagcgcccgg cggtgccagt ggaccactca ccattgtcga 93360 tgaaatcgtc atggtagttt ccgtacaggt aataaaatcc gtcgtcatcg cgggccacac 93420 caatccccgc cgtaaagtca ggggttttgt ttgctgtgga tcgctccgta cctgccttat 93480 cgtaagcacg gactttcgtg acgttcatcg ggaggcaatc cacctctttc aggaactcac 93540 gtttgaaata gtttgcacct tccgggcgag cgtcccagtt gccgtgaagc agacgggcgc 93600 ggtcaacatc gttcagacct tccaactggg caaggtactc cgggttgttg atcagcatcg 93660 gagggttgtc gtaaatggtg gaagagatga agctgaatga aacaggctta catttcttac 93720 cgaagcgttg cttcagttct tccttggtgg aaccccagat gtagttgtca tccacacgaa 93780 caaaccaacg gataactccg tcacgttcag ggatcggcag accattctca tccagatacc 93840 agtcaatcat ttcccggagc ttatgatccg ggtcggggtt acaagatacg accatacggg 93900 aagggtactt tgagtttgaa cgcaaacgcg acatcatgta ctcaagctgt tcccagtcaa 93960 actgggttcc ttcatccaca ccaatgaaag taaattccag accctggatg ttatacttat 94020 cgttgatgtg ctgcatggac tgccacttca ccgtcgcccc gttcgggaag gtcgcaatca 94080 tctcgttctc tttgaactta ggcttgacga ccggaggaag ctggttgtaa atatctttcg 94140 ctttgtcgaa cagaccgccc tgaccacgaa tctgaggcac tgtacggcgg aacatgatgc 94200 aagctgtacg aggatcatca atgatcaaca ggggcatcaa ctggagcagg taagatttgc 94260 cggagcctgc tgcgccaccc atgatgagga tctgtgcttt agattcgaga accatctgtt 94320 gcttacgact cgcgggtgcg aatgtagcgg acattaactc tcctgaataa ataaattgac 94380 atgcttcgat agtaggtata ttatcacaaa gtcacctaaa agaaaaggcc ctccgcgtat 94440 ggagagcctt ttggcacaat cacttaccct tctcgcagac ttccccaacc gggtcttgga 94500 agaggttgca gacgtattgc tggaggtctt cgagcctgcg ctcgactgtc gctgcccgca 94560 agttgttgtt acttataaca accgcgcctt caccgttgtc tgtacctttc ttgaggtact 94620 gctttggtgg agccttacgc attagctctc ggtccggctt gtctacaagg tcttctaccg 94680 tggtgccctt gaaggccacc tctgggattc tgtctttgtt gctacacgct gacagtgcca 94740 gaaccatgaa cattccgaga ataaatttcc tcatcagtcc cccaaggatt cgttgtacag 94800 atccacaaag tcatcgtcca accctttctt ccgggcggct tcagtcttgt tgtactcgac 94860 ctgcttatcg cttacgttgt gacgccgggc ttcttccagc ttgcgctttt cttcagagac 94920 ccgggccagt tcttcgttaa gagccgtgtt gtcagcctta cccttgtcga taaggtccac 94980 gtactctttc ccctttttct cccactgggt attggcagac tggaagccaa gagcataggc 95040 cgcatcttct ttggtgccga cataccagtt gaaggagaac agcagcagga ccgcgatccc 95100 gataccgatc agatacttct tgtacttatc aatgaaagta gccataatac ctcctttggg 95160 tattttatca ccgaaaccag aaataaaaaa gccctccgca aagggagggc actgaggcga 95220 aggagaggag atattttatt gttattggaa tttcgtaatc gtgatgtttt tcaacatccc 95280 gttcatgttg taagaatttg ttccgtagtg accgccgata cctacaggca ggccctcgga 95340 gtgtaccctg gaggcagcta tcgtcccgac gttgacgtcg tccatgaaaa tggtgatatt 95400 ctttccgtca aacttaaccc ggacattcac aggcgtcaga agggggacat ctcgatctgc 95460 aacgaccact tggcgggaag acaagctgcc attgaatgtg aagaaaaagg ccaatctttt 95520 cacgttcgtg acgcttgcca tcgtcaggcc aaaagttcct ccaccactgg agggactcaa 95580 ctgagagaat atagcacctt cggtggaagg atatccccac aggtacatgt cgagtttcaa 95640 ctcgaacggc ttggtcccga ttgcgaaatc tacattactg aggttttcga agtgacctcc 95700 tgcggattcc ataccgagga cttgatttcc gaaagcatgg cccacaacac ctgcggaacc 95760 tgtggcattg aattgccgaa ggcccgcaac gtccatgacc gttttagaac caccaccttc 95820 taccggggga atattaaaaa gcaaggtttc tgtttcttgg cgaccgatca aaagtaattc 95880 gttcattggg cacctgtgat tggtggaact gaacggattc gaaccgccga cgcgctgctc 95940 ttcaggcaac cgctctacca actgagctac agttccatgt ttggcagaag aggagagact 96000 tgaactctca ggccccgaag gaccccggat tagcagtccg gtgcggctac ctatttcgcc 96060 actcttctaa attggcggaa gataatagag tcgaactatc accgtggggt cgccacagcg 96120 ggccggggtt caaatccggg tgcgtaccac tacgccctat cttccattat ttgatgcttg 96180 tccaagccgt tcttgtgcgg agaagttgac ctgctgccct gaacagcgtc agaggtgcgg 96240 ctgaaactcc accttgggca ttggaccaag tttgaaattc ggtattactg actgcccgga 96300 acaacacttc tttctcatca acatacaaat gaacaccgtc tgatttcatt tcgtagctca 96360 agaggtgcca gcccttgttg ataggtactg tactgtcgag ttgcaccgga ttggagccgt 96420 tccatacata gaagcaaaga acagtggccc catttaagaa tttgacaccg aaggaccaat 96480 aatccaaggt gtctatgcct gcgaaaccta ccatagtggg gtagaagccg gaggggtaaa 96540 aatccacagc ggggtcaaga tacaccatag tgtctatcct ggctccacgt ctgatagcag 96600 acaggttcaa tccataattg tattggatga acccaccttg ggtgttctgt ataacagcgt 96660 gaccgtccac aacatccttg cttactccgc tcctgttggt ggagcttttg ttccctgcaa 96720 ggtcgggagt catagagttg tcggcaactg ccaaaaactg atacccggtc gggtctggag 96780 ggggaagata cttgcctcct tgcggggcag agacgatcat catttcgttc ataaacacct 96840 cagattgggg tgacctgtgg gactcgaacc cacgactgcc cggttcacaa ccggggattc 96900 tacctgctga attaaggcca cattgatgct cactaaaaca cacgtctgtg tcgcctgctc 96960 taagggttcg agttccactg acaagagcta atgagcatta atgtggcggg ggatggagga 97020 ttcgaacctc acgatgtcgg gatcaaaacc cgatgtttta ccaattaaac taatccccta 97080 cactgggtga gataacccga tcaccacggg caattgaatt ggagcggaca tccgggatcg 97140 aaccggactc attagcttgg aaggctaagg cacaaccact ataccatgcc cgctgggcgc 97200 tcaggtccga agaaatctga gccttgtgtt tggtcagagt ggagaggatc gaactcccga 97260 agacctgttc ccaaaacagg tggcataccg cttgcctaca ctctgcgtat tcctgcatgg 97320 acttctttat ggcagcagga gcaaagcagg acgcatttag agacctctga gaggtatgct 97380 gcccaactcc tgtggataac gtctgccacg ttgtcgtcct tgttatcgtc ggggtggtgc 97440 cactcaagag caccgccgta tgcgttgtat ccgcaaccga gacaacctat tgattcttta 97500 tgttccctca tccgcttaga gtacaagtca tttctggtct tgtttctatc gtaacgggaa 97560 aggccgtttg ggaggcagga gaagcagaat agtctgttct ttccgccgga gcctttctta 97620 gggatttcaa actctgccgt gcaataatca caaatcatat ttcctccttt tatggaagat 97680 taccatctgt ggttgcaggt gtcaagaaaa tggttgggga tggtggtttc gaaccaccga 97740 cctcacaatt atcagttgtg cgctctaccc ctgagctaat ccccatttga agtggcggtc 97800 ctaacgggat tcgaacccgt gactctcgcg tgacaggcga gtatgtttac cgctaatacc 97860 ataggacctg aatttggcag gaaggtgacg aatcgaacgc cttgaactgg ggttggaatc 97920 cagcgagcgg cctttgctca ttaatctacc gacctataat aattggttac ccggacaggg 97980 atcgaacctg tgtctccacg ttcattcgag cggtggacga cttaccgctt gtcgcaccgg 98040 gtaattgtat ggcgtcctga gaggggatcg aaccctcaaa ctccaccttg aaagggtggt 98100 gactttatcc agtttgtcta ccaggactta acgaatagtc atcgcaatga ctattgatta 98160 agtattggct ggtcctccgg gactcgaacc cggataaacg gattaacagt ccgccgtaat 98220 aaccgattat acgaaagacc aataattggg tgcgcccctt ccggtgtgac tgagaactgt 98280 acccgacagg cggggaagta ttactcaatc agggaccttc ggttggacga ctttagggtg 98340 gcctccatct gggctttggt ctgaggactc gcctctcgca ttaccagctt ggaccctctt 98400 cctttctggt ggaccgtcag ggactcgaac cccgaacctc gggattaaaa gtcccttgct 98460 ctaaccaatt gagctaacga tccgaattgg aggtaggaga gagatttgaa ctctcgggcc 98520 gtggggttac cacgaccgac acctttccaa gatgctgctt taagccactc agccatccta 98580 ccttaaattt ggtgccgagg gacgggatcg aaccgcccga gtcgcaatga cagcagattt 98640 acagtctgcc ccgctacctt ctacggtcta cctcggcaaa attctctttt gtacggctca 98700 cctgacatcc aaggatctgc gctgatccct atagaccttc ctcgccatca aacgccgttg 98760 tacaccgtcg tcattctgcg ggtcgttaac aagtgcccgt caaaagagaa cttctaataa 98820 aggctccttc gggagccttc cagttaatca tcttcgccta ctgctttata ctcacgaatc 98880 tgggttgtca acgggatggc atttttcatc tcgtcagaag aaccagcgtg agtttcagaa 98940 gaggcagatg ttgtgccgcc tgctgcgtcg tcttcgttat cctcttcagt atagaggttg 99000 ttaacctctt tcgcctgctc aaggatatac ttcgccgtgg tttccttcac tttctctgtg 99060 cctgttgtga gcaggttttc gagagtatga atagccttcg gcgtcagacg ggccaattcg 99120 cctcggagat caagcattgc cttttcaagg gcgattgctg tgctctcttc tttgcttggg 99180 cgaccacgct tacgagttac aatcgcagcc ttgctcataa tgcccccatt gaattagtgt 99240 atccatatta gcagtataat tacactatgt caataggtta cataaatttt ggtgcgcctt 99300 cccagattcg aactgggaac ccctacattt tgaatgtagt gcctctacca attggaccaa 99360 aggcgcttgg agcaacgtac tggattcgaa ccagtgctca tctcgtttgc agcgagagcc 99420 gttaccagac tccggcaacg ttgcatgatc tggtgctgga tgcaggattc gaacctcgcg 99480 acatcctcat tacaagtgag gcgctctacc tactgagcta atccagcaag tctcccagca 99540 agtccggtag ttgttacgct ccgggggagg gagaaatggg cagaggacgt atgcccgtgg 99600 ggttgacttc cccttctcgt ccagcaaaag cgtgtttcac gtaacgggtg gtcgtgagac 99660 ctgaagcaac cgtcgctatt ccagaggccg tcgatggagc cgctgtttat ccggctgaac 99720 tcaacccgaa ccgcaatggc actttggtcg tttaacagac cgggttgact tatccatcca 99780 cctacttgat tgtcatctcg ttcgggacat attcagatta cctgtagccc tactctttgt 99840 caacaggctt tttaccgttt gcacaaagat tgcacataat tgtgcgtttt gggccgaggt 99900 acttgtttcc gcacacgtaa caaaggcaga tgtatccctt gtattcgtgg ttccgggggt 99960 catccgcccc cggcaggtga ttaaaagtcc gggtgctcat cttcgttaca cccagagcca 100020 ataccttcac cccaacctac gaaaccgtcg aactcatcaa acatcccagt tctcctctcc 100080 aaggccccag tcctcttcgc tacgaatgcc ttctccgtag ccgatcacta attcggtgct 100140 gttcatttgc attctcctgc gaaaacagtt ttaactttgc tgttgccagc gtcaccgaga 100200 ccttcttgta aagcctcgtc aataccttcg gtcatcttct ctgcgaaaac acttacgaag 100260 ttttccgggg ctactccttc gaacgtctcg cagtcgataa ggtccggctg gtccttaatc 100320 atctgctcaa ggtggtcatc atccagatcg atggtgacta agtaatggaa ctttctcaaa 100380 cttcctcctt caggattgcc agcttatagc gagaggtttt atcaacctca ttcgggtaaa 100440 acgagtatag gtgattacct tcaccaaagt caacattttg ccaaccctca agagggaatc 100500 cgacggtact gaaagctaaa tcgtcggcgt catcgagagc gtcttgaagg gtggggtaat 100560 gctcacaatg ttcttcagaa gtgtaatcgg gggtgataaa gctgagagtg agtttatagg 100620 gattcataag tccaacagta agttaatcaa gtgatatggt cattttactg gaggacttga 100680 tgatagtcaa cgcccgggtg gaaggggagc accgggggca tttgtggagg cacgttcata 100740 ttcgctgtat gcgacgttct cagcgttttc tgcgtagtag tcaagcaaac cctcatctac 100800 ggtctgtcgt ggctccttgg agcgatccag gtgggttgct gcacggttcc tgtcatgttt 100860 tgcgacaagg tttctcattt atcctccgga tagtgaaaaa accgccccga agggcggctg 100920 aaattatttc aggtttgctg tgacacgctc acggatttca gtcagcgttt cgtcaaccaa 100980 cagcttgcca tcacgaaaca cgacacgaag ttcgttatcg gcatcgttga cttgctccag 101040 ttggaggtta tcaaccagct tcagttcgcc gtcttcacca cgaacaacct tcaggaagcc 101100 ctgtgcagac ttcttgccga ggtcggtctt cggctctttg acgaccatga tctgctgacc 101160 gtcgataacc gcaccagtag ccttcacggc ctgacccaga gaatcacggg tgttgaactg 101220 ggtggtgaag gaaccgaccc cgaacacaac gttgttcgat gcaaagcctt ttgcttcaag 101280 gcgctcgaag atctctttcg cacgggccac ggtgatggag tcaccgtaaa tcaggccgac 101340 gtgagtatcg agtgtacgat agcctttgga gttcaggtcg ccgccgaaca catcgtacag 101400 cagttcaaca gagcctttct gctcgttggt cagttccgct ttttcgtaag acagaacacg 101460 actaccgtcg atgtagtaat actgcttgtc ataacggttc cagtcaagct cacccttcat 101520 caggtacagg tcaccatcga acaggaagta acgttcaaca gtctcatcac cacattcacc 101580 gtgcggtgtg tcttggcgaa cttcttcttc cgcactttcc agcgcccact ctgcggcacg 101640 ttcgagagtg cccgcagcgt ccagttcttc aatctttctc cagtcgagtg ttccgcagat 101700 gatgtcttct ggaactccgg agtccggacg gaataccaca cgaccgtcac gggccatgat 101760 ttcgtctttc aggatgcgaa caccctcggt gatcagacgg aaccagtcgt agctgtcgcc 101820 gacgtatgac acgaaacctt tcgggaagat ttcagtgatg tatcggcgca ggaactcgac 101880 ttctgcgtgg aaacgcaggt catccagcga catttccgga tgctcagaac ggaggcgtcc 101940 gatgatgaac ccgatgttcg ttgttgcaac gctgtgctcg gatgccggga tggagccgga 102000 tacgaacttg cctttcatac cgtaataacg gtttgcatac aggatcgccg ggatggtgtc 102060 ggtgccctga gagttcatca gttgtcccag ttcagactgt gccgcatcgt gagtaccagc 102120 ctggccgcga taagcgaagc agtgaatctg gaacggaaca tgcaggttgt tgtcgcaggt 102180 tttctgcgcc cacagttcag ccagcttgcg atactgccag aaaatcgttg ccgtggttac 102240 ggttttccag ttttcagagg agatcactga ctccagatag tttgggagcc agtagaagtc 102300 cgggtgcgtg ttctgaattg tgaacagagg cacttgtacc ggagaaagag taccttccgg 102360 cagggcgcgg atttcaacag gcagatagcc caggtcatgc agcttttcga agccccacgt 102420 ttcaaccatg tctggaccga tcgccaggtc acagatctct ttgaactccc cgataacttc 102480 ttccttcgga cgaccgaaga actcacggtt gaagtgttca atcaggaacc acttaataaa 102540 gccttgcagg ccagcagaca tgacgcgctg gtcactgtca gaaaagtgct gaaagtgttt 102600 gcttgagcgt ggtgtcatgt tgtcttgtac gatctcagac tgtttgctgt acatggagat 102660 atggctcggt ttgtaccagt cagcaaacag gataggcagg tcgttatgta acatataaat 102720 ctcctcagtt aaattggggc aatgtgcccc ggaacgttgt gaatattact tgaactcaac 102780 catcttgtca acagatttga aaatattatt ttcgtttcgg ccttccaggt tcttcatcca 102840 cggataggcg gagtaaacct catcaataac gtccttaaac acatccgcgc cgtaggaata 102900 aatcccgtgc gtggtgtaca gaacaacctc tttcgcacca ttggcacgga gaaccttacc 102960 aagctcgata aatgtacgac ctccgtcgca aatgtcatca accatgatga cacgcttacc 103020 attaacatcc ggcccggcat aagtcgtgtc agtaatgtta ttggtcataa ggtcacggtg 103080 cttggtccca accccgattt cacggtggtt gaccttctga gccaacttcg tgaccttctt 103140 gattgccccg gcgtccggag ctacgataac ggcatcgtcg aagaagcccg ggccaagaat 103200 atccaaggcc agttcatatt gttcgaaaat ggcaacgttc ttgatgtgtg acggggacac 103260 atctgagtgt gggtcgtcaa caatgatacg gtcaaatcca cacaggttga taagtgttcc 103320 aaacactgcc gacgacagcg gttcgccagg aaccatcact cggtcttgac gggcatacgg 103380 gaggtatggc acataacaga ccttcttcac ttggttgcct gtgccgcgac ccatacggtt 103440 gaatgcgtcg gtaaacagca gcaggtccat gatggcatcg gaactgtcga tgtgggcgta 103500 gacaacgagg tctttgcctt ccggcatctt ctctttcagg tcaaccccgt atgcagggga 103560 atgctggaag cctttgatgc ggacgttacg ttcaccgcca gcgaagccag tgaactcaat 103620 gtcgagggct acaccgccca gaaatgcacg aatcatattt ctctcctctc agttaaagta 103680 cagcgatctt acaaaaagaa aaaggccccg tcaagggcct tttgttagag tggtgtaatt 103740 ttaagacttt tcaggcgtcc cttccaagag gcaccgtatg tgtacgaccc gccaaccgac 103800 agcttgcttc cattgccgat cgggcttgga cccaaggcaa ccctggagaa ctcggcaccg 103860 tcgcgataaa caaccattgg tcccgttcca tcatttgacc gttggaaggt tacagtctcc 103920 cattccccgg tgaaggttgt agctggaggt aggccacggc cccaagaccc gttggtatac 103980 atcatgaaat actggatgta cgtagcaggg tattgaccaa cactaaggca aatgccgggt 104040 gttggtggtc gtccactggg gtagttccca gtctcccaga cagaccctcc tgcggcaccg 104100 tcccacttaa ataccgcctc gatcatgaca ggtactccag cgagttgaag gtcctttacc 104160 ataggggttt cgaagtatcc cgtaccgggg aagtagaagc acttccctac ctcgggtctt 104220 cgactacccc accaccagtc gcgatagtac caacgagtgt gaaaggatgt ccgttcttgt 104280 ccacaatcgt ggtgctgcca atctcggcgt cttcaaaatc aatatccagg ccagctttag 104340 ctttggcctg cccaagcata agcatttcgt tcatgagttc ctccgccccc attttatcaa 104400 gattcagtca gatagtctaa gaaagaccat acctttccaa taacacaggg tcattgacca 104460 caatgtagtg ccgtggcttc attgaaccgt acagcattac tttctcacgt atgatggaaa 104520 tgtggcccga tgcctccatt cgcttgagaa actttccaac cttcgtcctt gatgcttcag 104580 acataccaaa caggcgacac atggtctcct gtgactcata gtaacaacgc ctaacgtctt 104640 caccactctg aaacgcttgc tctgcaaaat acttgaatcg gaaatgtaac aagaacatcc 104700 gcttcaggtc ttcagcaggc aagtcgaagc cccacttagt agggaggctt acaaaagaac 104760 cctctggaac aacaaaatcc ttcacatacc ctcctttgaa accctaacca gtgtttcatt 104820 aatcaaatcg gttgtcaacc atgtagccct gaaaggcaca cgaagtaata atagaatcta 104880 taaacctcgc tacgctcgcc ctccgggcta cggttttatt aatcttataa tttattcgtt 104940 ccactcataa attattgatc ttataattat tattcatata cattcataat aattattaaa 105000 aacaagaaaa caataggaga ttcctgttga tctttaggat ctttatatag atcttacatg 105060 aaaaatttaa aatttaaagt attgacaaga gggtcattgg atcatagaat gcacacatcg 105120 aaacgagaca accatacgag ggttatagaa tgttaagtaa taagcaacgt aataaaattg 105180 atgaactgga attagcccat ctggaagctg tgcgtgaggc atggcactca gcaaaagctg 105240 gcctgaaagt ctgcatggtg tctgtttacg agtgtggatg ggacacccta ttggggaaat 105300 attacgatgt gatgatcaaa tatgtggaaa aggggttgat ttatcatcgt gtggaggata 105360 gtatgaagtc tgaactccag cttactgctg atgacctcga caatgtactg attacgatgg 105420 agatgatctg atgaaacagt gtgtgaatga tattgtggct ttcggtatga acgataccga 105480 actccgtgtg gttgccactg tggttgccac tgtggctgcg tccgctgcca ttgtgtacct 105540 attcaaactt atccacaaat actcataagg atcaggtcat ggaacagtgc gtaaacaatg 105600 tcgttgcctt cggtctgaat ggtctggagt tctgcaccgt tatcggtgtg gctttggtgg 105660 cccttgcggt tgtcctgatt cgttacttga atcatggtat cggggagacc aaggaggagc 105720 gctgggcgcg gaagaacccg tttctggcgg tggacttgga catgcaccct cgtgggaaga 105780 aacgtgatgt gtggtaactg ttctgatggt tcataccctt actacggcgt agggccgcat 105840 atctgcttct acaagatacc gggtgcggtg atagggcaat caataacttt gtccacagca 105900 cctccgaatt acgtggagga ttctgactgc ccggactggg tgtatggtat tgcccggagt 105960 gtcgggcagg catgaaggag acataatggc tttacatttc aaagatggcg tcgttgatcg 106020 gatggaagag atctaccggg cacaagagaa gcctcgcttc tacaagtgga cgatagacct 106080 gaatgaccag tgggcgtacc tcgaagagcg gatacaccgc gccgggattg acttcactgt 106140 gacgagagtc cggcagaact gtgacaatat gcacgtcacg atcgatggtg acccggatca 106200 ggttgcatgg atggacatgg cccttctggt ctgggcaaac aacaagcggt gcaaacacct 106260 gctggtactg gagcacaagg gatgatggaa actattgttg catataagtt gtttcgtgtg 106320 cgaaaggatg gtacgatagg acccctattc atcaacagga agctcgtggt ggcccctggt 106380 gaatggatgg aggcagagtg ccacccaacg aaaggtttcg ccctacggcc cggctggcac 106440 tgcacactgg cccctgtggc ccctcacctt agcaccaacc tgaaatccgg tgaagtgcgt 106500 aagtggttta aggtggaggt tcagggaatt acgaccttcc agcgcccgga gtcccaaggt 106560 gggacgtggg tcctggcaga aagactccgt gtcgtggagg aagtgaatgt gtgattgcga 106620 aaggtgcaaa ctcggacgcc gagtgaaggc gctccgggaa aggctgatcg aaaccgattc 106680 tggtcgccat gtcgagacac tcgacgatct gtactcggaa tacgtacacg acagcatgga 106740 ccatgaccat ctgaaggcga tcgtggacaa ccaatggccc tctgcggagg cgtatatgaa 106800 atcgaagggg tgggttcgtg ggtaagagtt tatctgaaaa gtggcttgac gccgggatcg 106860 ataagtgcct taatcctccg gtagaggaga ccgaggatat gctggtcatg gttgacgaag 106920 agccggaata tgactcccca cacgggagtg tgtggatcgt ggcggtggat tatgctggga 106980 gggttaccgt gctggaccca ccaaacctgc atcccggtat catggacaac ggcccggagg 107040 cggagtatct tggcctgccg ccggacgtag atgacatgga ccctggtgtt tatcgctgga 107100 cgtgcagtta ccacacccat attgaccggg agtctggtca tgtggaagac tacgactttg 107160 aggtcgggga gtctgttttg ctctggtcac cagataatgc ttgacgcgat ccgatttctt 107220 gcggtattct taattctcgg cggggcaatg ctcgccgggc ttacaattgt ggcgttaatg 107280 aggagaccaa atgtcaaacg taacgagtat tctcgcagag ttgtctgcaa cccgatctct 107340 caaggagaag gaagcgatcc tgcgggctaa tgccgataac gatggcctaa aacaagcctt 107400 cgctgttgca tattccaaag aactgaactt tttcgtccga ggactgcggg ttccattctt 107460 cacgactggt cttaccccgc tgtctgaaag cgtgggtctg ctggtgaaga acatcgcagg 107520 gcgagtttac acaggtgatg atgctaagtc gtacatgact caactggtac agacctgcga 107580 ggagccaggc acacttctga agatcatcaa ccgagatctg gagtgtggca tccagaccac 107640 tcttaccaat aaggtgtgga aagacctgat cacggagcct ccgtaccaga gctacaccct 107700 gttcaaagag gatctgctgc gtaagttcaa ctacaagggc gctttctccg acgagaaaat 107760 ggacggcctg tatgcggaca tcatggtgtg gcctgagcag gtgatctacc gttcccgctc 107820 cgggaaggaa ctgaacttcc gtgcgccgga agagatcgag cagcgcctga tgcaggcggc 107880 agaaaaccgt ggggcctttg tggcacacgg cgaaggtctg gttatcgacg atgacggcct 107940 gcatggtgtg atggaacgtg ccgagggcaa cggctacctg aaccaagacc cggcagacat 108000 cgatcgtgat ctggtgcgct tggtgatctg ggacatcgtg acgatggaag agtacatcgc 108060 acgtaagtcg aagaccgact acgctgatcg tcgtatcgag gcggatctgc tggtgcagga 108120 agtcgatcgc cgttacaact tccgtatggt cacgggccgt aagatcaaca gcctgaagga 108180 agcgatcgac cacttcatcg aaatgcgcct ccagaagaaa gagggcaccg tcctcaaaga 108240 ggcgaagatg ccgtggggcg acaacaagac gaagaaaggc gtcaagttga agaacgagtt 108300 cgatgttgat ctggaagtcg tggctacaac gccgcacaag aaagatcctg gtctcgtcgg 108360 cgcactgatc tgtcgtactc gtgacggctt gctggaagtt ggcgtcggct ccggcttgac 108420 ggacgcgctt cgtaagaagc cagcggactt cttcatcggg cagatcatca cgatcaaggc 108480 caacgacatc acgaagtcgg aaaccaaaga gcttcagtct ctgttcctgc ctcgtctgaa 108540 ctacaagttc gtcgagatcc gtatggacaa gaccaaggct aacagcctgg aagaggtgat 108600 cgaggcatct gactccctgc tggatctgct gcgggctatt gcggaggatc tggaatgacg 108660 ggattctggg tctgcacaag tttgtttatt cttctggtcc tcgtatgggc atgggataag 108720 tacgtgacac caaggcgcat gacccggccc ccggtcaatc ggattctcga attgatcgct 108780 gccaaaaagc tggaagtgta cgacaaccca tcgcaagctg accgagaccg ctgtagccac 108840 tttgtggact atgtgatcct cgaccacgaa cggaaggtga agacgtacct ttaccgtgac 108900 aacttccacg gctcccctgt tgtctccggg gatctcgact ggatgaaccg ttgggagcag 108960 gatcgtgttc tgcgtctggt cgaaggtatc tgcggtgccc ggcggaaagc ctaccgggaa 109020 gcgtgtgcca tcgaaaggaa ggcccgtgac gaacgtcgcc gggaagaagc agaaaagatt 109080 tacaaaagtc gttgacagtg taacggggtc atgtataatg gccccactga aacagaaaag 109140 gagaagttga tgtcgcagtt tattaaagat gctatccgta ccgagtccca caacttttat 109200 ctggacgcgg ttggcccgcg cctgattcac gcttcaatgg gtctggtgac cgaggcggca 109260 gagttccagg atgccctgaa gaagtccctg ttctacggca aacctctgga taccaccaac 109320 ctgaaagagg aaatgggtga cctgctgtgg tatctggcac tggcgatgga tgcactggaa 109380 acagacttcg atgcagagca ggaccgtgtg atccgcaagc tgaaggcgcg atacccgagc 109440 aagttcgata atgaactggc agagaaccgt gatctggcta ctgagcgtaa gatcctggag 109500 acagacaagt aatgcctacg tattcttaca aatgccccgg gtgcggggca gtatttgaca 109560 agaatcaaaa gattgcagag cgcgaaactg ccccttgcaa ttgcggagct actgcggtaa 109620 agtccctgtc tgctccccgt ggtattaaaa acggatacta cgagcaagga cgaatgttcg 109680 tccgatagtt tcaaaaagtt gttgacacac tgagttaaaa cgagcataat gctcacacat 109740 tcactgaaga ggagtaacac aaatggctaa agtaaaagtt gaactgctga acaccctgcc 109800 ttctgacccg gctgtacgca agcgccttgc aaatatcgtt caggaaggtg ttgatgcgat 109860 tcgtaagcag aaggacgccg cagaagacct gaaaagcatc ctggcgatca ccaaagaaga 109920 tctgaacatc gatccgaaat tcctgcgcac cctgatcaac ggtgagtacg acaagcagga 109980 cgccgccgag aagaaacgtg cggcgctgga aggccaaatc gagcagctta acgaactgga 110040 catcctgatg ggccgcgctt ctgaagaaga cggcgagtaa taccccgctg tgcagtaccc 110100 cgcccctacg ggggcggtta acccaaacat aattcagaaa ggagatttac aatatgaaca 110160 ttaacttcaa cgatgcaacc ttcgaaaaac tgcgtttcct ggctgaccgt gaaggtcgtt 110220 ccgtcgccgc cttcgtttcc atgcgtatgg atgaactggc tggacaactg gtcaacacca 110280 cgttcaccat caacgaaatt ccgccgggtg ttaagctggc ccatctggac ccgacaccgc 110340 gcccggcccc agtccgtagc gagcagtaca actttacgct aaaggacgtg gataacacag 110400 aaaatcagcg ccgctacgct gaagatcaat actaaaagaa ccgcccttcg gggcggtttt 110460 tctctatctg cctcttgact cctgcctccc atcctgtata ctactcccat caaacaacga 110520 aagaggaaat atcatgagca acagtatcaa cctggccttc attgaaaagt ggatcaacaa 110580 cgtatctatc taccctccgg cagagatctt cttccaggct ccaaagagcc aagagaagaa 110640 ggagaagcgc tgcaaggaaa tctgtgagtc tctttaccgc ttcaactggg aaaacgcccc 110700 ggtagccaac ccgatcggca ccgttcgtga gatggtccgc gagatggtta ttctggataa 110760 gatgttcgag tacaacccgt ttccaccgaa gtacgaaaat attgtgaaat tggttgcaaa 110820 gttcctgaat catgcgtatt atcttattga ccagttggac agtatgcaca aagagcacga 110880 caaactgagt gcatggtacg gtgaatggcg gcgcgggccg ctgcacatca tgatgaacgc 110940 caaccgctgg ggtgccgaca tcctccgcct gccgggcgat gacaagacgc agactcagta 111000 tttggacgac atagcgaaag agatgggggc aaagtgagta cagaaatgaa atggcgagac 111060 cttgcgcttc agttcgatgg gcaccgtatg caggctttgt gttttctgaa acagatcctg 111120 aatgcacttc cggagactga atttccagag gcccgtgaat ttattaaggc cgggccaata 111180 ccaggcgaag agatccttcg cgaacgaatc gaaaatatcg caaaaggggt gacgaaatga 111240 aagaagtatt cttagggttt atgctgggag ttctgattac aggtgtctta aatttctctg 111300 cggcacatgc ggctcaaacg ctggacctgg aggattacta caccgacggt agtgacaagg 111360 tgtgcattta tagcaacgga cgcaccaccg tggcgataac tcacgaaggt ggcggttctt 111420 gcccttcaaa atacgttaag cactaagggg acaccatgct aatcaaattc aaccgagagc 111480 agatcgcagc actcctgaaa aaccagtctg tccagtctgg tatcgacaag gcaagccgag 111540 acataaaact gccagagatg aacaccacgg cggctgtgct ggagcacata ggggcgaaga 111600 tcctcagtaa gttggagaat tgccagtgaa atatttcaaa ctgcactccg cacacgaacc 111660 ttggctccgt gctgaaattg ccaaagggac catcttttac gacatgcaca acggttttcg 111720 tggtattgag ggccggagaa tgatctccac ggctgcttac cgtgaatcag gtcggtcata 111780 tgacgacatg cctgtgagag aggttgtgcg cctcatcaag ctgtacaaaa atgtttactg 111840 gaggaactga tggaaaacca aatcaaagac gacctgccgg acgtgtgcta caagattgct 111900 gactggtggg acggacgtaa actccaacgc cggatcgttt gtgccgccaa ccgattccag 111960 ttgaaagatg gcggggaact ggtaatccct ggtgttcgcc actattcgaa agatatggcc 112020 ttggttctgg accaacttcg tgataaagtg gtcagcgacc acgtaacggg agacgaccaa 112080 gggttcgttg accagtgggg cgattaccac acccgggagg tggcagttat tatcgccgaa 112140 catgcaggcc agcttaatac ggtctgcaag aagtccggtc cggtggacga actctttagc 112200 gaggatcttt actgatggaa actgtaacca ttgaggtgtt tgctgtggat atgtcaaaag 112260 cggttggttt ccgtaagatg gagccgactg gcccggctgg gctgcgcttt ggtctttacc 112320 tcccgtcgaa aggtgagccg ctgattacct tcgggcagtt gatggacatc gtggagaccc 112380 acatcgtgaa cagtgcgctg ttcccgtgga agatctctta catccacatc aatgccgggc 112440 agtatagcaa caattcaagc atcctgttgg cgcaatacct ccaggagttc ccgttagcaa 112500 aagactgccg gatcagttgc ccggcgtccc tgatttttgg agatggatta tgagagcttt 112560 aatttggggt ggtgtgtacg ttctgtttgt catcttcgtg gcctgcctga tcccggcagt 112620 gatgattccg gtattccaag acttcggcct gtacgcccgc gatgttgtag ggttcaccct 112680 gaccatcaca ctggtcgctg ttctgctttt gatggcattc gggcactgat atggagcgtt 112740 ctgtcatgaa agtgatccac ccaatcaaga atggcatgtt cccgatccag actgtcctgc 112800 gggaccttgc gagtcaggag aactgcgacg gcgatccgta tgaccagatg attcaggctg 112860 cggatcatat cgacgacctg gaggtccgcc ttgagttctt ccgggaaaag tacaatcgac 112920 tggcagggga gttctgcgta tgagagtgac cgacaagcac gtgttcttct attcgtggaa 112980 ggacatgttc agtaatcatt accgaaatga ccgggctttc gctctgccgc gccatgagca 113040 ggaaggtgtg aagttctaca ccggggagca catgatgatg tacgagaagg ccatgctgtt 113100 tgccgatcac aatatcgccc gtcagatcac cgaggtcttc cacccgcagg aagccaagat 113160 gcttggtcgt aaggtgaagg gtttcaacaa tgagttgtgg gagaagtccc gggagaacat 113220 tgttgagaat gtgtgctatt gtcgccttgt gtacgacctg gatcttcggg gcgctgccat 113280 cattcacaga ctagctggca ggagtttcgt agaggcttca ccacgtgacc gcatctgggg 113340 gatcgggatg gatgagtttc acccgatgat taacgaagag cagaattggc tgggccttaa 113400 cctactgggt aaggcttggg accgggcgac tgacgccctt attgaccgtt gcggcggtta 113460 tgaccgagtg agaggtgact ttgcagatca agcataaaat ttgtctaatc ccacagactg 113520 cctgggataa caatcttcgc tcctacctga ctcaagccgg gtgggataaa gttcgccgca 113580 aatgctatgc agagcataac tataagtgcc agatctgtgg cggtcagggc ggcaacggaa 113640 gaaaacatcc agttgagtgc catgaaagct ggacattcga aaatggagag gttaaactgg 113700 tcggcctcat gagcctgtgt ccgccgtgcc atgagttcca ccacccgggc ctcgccgaga 113760 agaacgggaa actggaaagg gcgatcaagc aattcatgaa ggttaatggg atcactcgtg 113820 atcaggccat tgcctatctg aaaagtgagt ttgccacatg gcggcagcgc agccagcagg 113880 agtggtcgct taatatcgac tacctgagtg agtacatggg gccggacttt aagttcaagc 113940 gccccgtcaa ggaagaagag gactttggtg atgcgtttta agaggagtga gtaatgggtt 114000 atcccgttgt agaaaacctg aaggatatcg ttacggatga tgttactatt gttcttgatt 114060 tggatcagac gtgttatgtt gcggcggcgg gcgcggaaaa gcgtaccatt atcgcaaccc 114120 acaacaaatc cggtcgtcag atggagttca agaacaagac ggagttttgg ggccgacaga 114180 aaacagtagt cggcgggtgg ctgaaggacc agaacaccaa catggaggcg aagatggcgg 114240 cagcgggccg ggagttcact ccttggaccc gcgatgattt cacgattacg gacaagcagt 114300 acccggaacc cgtggaaaac tgcctgcatc tgttgaagat taagatcaac gcgatccttg 114360 agcatatggg gaccaccaac ggccttggtg tcttgggtgg gagcggaaac ttccggttgg 114420 cactgcctac gccggaaatc tacaaaggca accgtgagga cactgttcgc ccgttgttgc 114480 tgtcggatac ccgtgaatat gttcaacgga aatacggcgc gatcgtgatt gacgggatcg 114540 aggcggatga ttatctgacc atgaaacagt atgagggctg gttgcattac aaaaagactg 114600 gcaagttcaa ctatatcgtg gcatcattcg acaaagatca agtccagtgc cctggcctta 114660 tcttcaatac catgcgtgac tcaacagagc gcgactggaa gcatccgctt ccgtggctta 114720 ttgatgagtc gatgggggag atctggatgg agaagggtaa ggtaaaaggc tggggccaga 114780 agttctttgg ttaccaaatg ttgttcggtg atcaatcgga taacgtgaag ccataccaaa 114840 acttcgacaa gatcaagttt ggcgagacct ctgccttcca agtcatctct ccgtgtcagg 114900 atgagaaggc catgtgggaa gcgatcgtaa atcagtacaa ggagtggttc cctgacgggg 114960 tggcattcac tgatcacatg ggcgtggatc gtaagttcag cgccgggcag tgggcctctg 115020 ttatcttcca agcggtgtac atgaagcgca ccccggatga taagaccact ctgggacatg 115080 ttcttcgtaa agtgggggtt gtgtaatgag tcaaaacgac gctggcggtt tggatgttcg 115140 cgtcgaggag atgattaagg cagatgaact ccgggcggca ttcgtgcccg gggcttcaaa 115200 gaccttctat ctcatcgagg agccgtggac catgctggaa gaaccttggc gaatccagtg 115260 gtacgaccca cgggtgcgta gtctgtgcga agtatacccg gtgggtgttc cttacccggg 115320 ggacaagtac gccatgttca cggtgtacga cgaagttcaa aaggtcatca agaactatgt 115380 gtttttgatg aacgagtacc attttattgc taaggatgaa gatgcgctta ttgacgattg 115440 accaaagttt gtcttcatgt gcctatgtgg tgttggaaga cgggattccg atctttaagg 115500 aggtactcca caccacgggg tcagagaaga aggccaagga ctccaaaggt tggtgcatgt 115560 acttcgagca cactgctgaa cagattgcct acatcaccgg gcagattgcc ggggtgtgtg 115620 attcgttcga tgtggatcac atcgtgcttg aatcgttgag tttaggttct gttggtgatg 115680 ccacaaggga tttggctgga ttattttaca gtatccagtt gactcttctt cgtgacggat 115740 atactatgga ccaaattcac gtcatcgcac cgacatctgt taagtcttgg gcacgtaagt 115800 ggttgccggg ggaagagcag gaaaccctga acgactccgg gaccaagatg gtgaaggtga 115860 agatgggcaa aaacgaaatg atgcgtgttt gtgagatcct gcacccgggg ttcctgaagg 115920 gctataacaa gtccggaaag aatggcgggg ctaccgacct cgccgacgcc atcctgatcg 115980 gagggtgttt ccttgagaga aacggagaaa gactgggatt caatcttaaa gaaagcaaca 116040 cgtaagatta tcctgtgtta cgaccggagt gataagcagg actggaggtt ttctttgcag 116100 gggactcttt cccgcgataa aacatacgag tctgtagaca ggaaacactt cgacgagatc 116160 attgttcgtg acaaggtgct gaatgttgat ttcctgatca acttttactg cactaatcaa 116220 tggtcttggg cagagaagac aaggggcctc atctatgatg aggtcctcgt caccgggcgg 116280 gggtttgaga ggatggagta tcctgaaaac ctagcggagg tgatgtctcg ttgcaaaagg 116340 tcagaataca taccgggcat ggctatgttc ctcttaacgc aaaacgacga gaggatcagg 116400 aacgggctgg gttcagatat tgccctgacc agtacaggtg tcggtacaaa tgtcggatca 116460 ctgtcggata agtttgactt tcctgttacg ctgttccgcc ggaccatgct gggatatttt 116520 gaggacttca aaaacccgac ccgggaggag ttccttttcg ctctgacgat ggagggtatt 116580 agtggcaata cggacaggga gaagaaggcg atcgaggaag cccagcttca ctgctaaagt 116640 gaaagctaca acgccgattc gtgtaccttt gagcaaggac aagtgggtgt ctgggtcgaa 116700 gagtattgcg gcctacaggg atcaactcct tttggagcag ggtggccttt gtgctatcct 116760 gcgggagccg atgacggacc catgtttgga ccatgaccat tacgacggaa aatgccgtgg 116820 ggtcatcggg tcaactctta atcttttcga gggcggagtc cagaagttgt ggtctaagca 116880 catggaagga aaaacagggt tgacaatgag tgaaactctg cgtagactgg cagactatct 116940 tgaaagggac tatctccacc ataagttcca cggagagatt gtcgccgact tgaagaagtc 117000 actcaagagg tggacaaagg aaactatcgc ccgtaacgcc tacctgaact tcggtatcgt 117060 tattgacgag aacctggaca agggcgtgat gatcaccatc tacgtcactg agtttgtccg 117120 gaaattggag gaagactatc tttatgagta agatcgatcg aagtctgtat gcggggttgg 117180 atgccccgaa cattcggttc ttctaccgta actggagagg tgaatgcggt tatcgaacca 117240 tcaagggtgc cccaatgttc tggtacgggg aaagtgagta ccataaaggc gctcagtggt 117300 tcatcaaggc gcacgatgtc gataaagacg atgttcgtga cttcgccgtg gtagacatta 117360 tcgaattttg taagtaatcg ggagattaat tttggctaat gtgactttta ctgttgaaca 117420 ggttatggct ctgatgaagg gtgggttgtc ccagcgcgag gtagcccgtc aactgggcaa 117480 ggaatctcaa gagtcaactg tacgcaacat gctgaagcgt gaactggaag cacgtaatct 117540 ggaaactgtc caacagttga ttgacagtat cccaacacca gacgagtacg ctgattttat 117600 gcagcaggaa gtcaatggtg gtaaaacgct ggcagagatc cagaaaacct tcatcgaaaa 117660 ggtgaaggaa aataacccgg gtgccaaagt gttccttggt gatgtcgaga cctctccaag 117720 catcgcctat tcgtttggtc gcttcaagca attcatcgct ccgaagcagg ttattcaaga 117780 gccgtacatg ctgacgttcg ccgggaagtg gctgcacaac ccttcaatct tcagccgtaa 117840 gctgaccgac tacccggaag agttcaaaag ggatcatcag tcggatcgta aattgatcga 117900 agacctctgg caggttctgg acgagtgtga tatcttcatt gcacataacg cccgcttcga 117960 ccgtggttgg gcgaatcagc gcttcgcata ttacggtatg caaccgccaa gtccgtatat 118020 cacgattgat accctggcgg cactgaagga gtcgttctct ctgccttcca atgcactgga 118080 agcggcgtgt aactacttcg aactggacag ccgtaagttg tcccacgaag gattcggtct 118140 gtggcgtcgc tgtgtggaag gtgatcccac ggcgttcgat gaaatggaag aatacaacat 118200 cggggatatt ccgacgctgg aagaactgta cctgactgtt cgaccgttta tgcgtaagca 118260 cccgaacatc gccctgttcc acccggatca gtctgtgatg cgctgtgtac gctgcggctc 118320 tgacgatttg gaggaagaaa aaggcaaatc tgcccacacc ttcctgtcca agttcaaggt 118380 gtaccgttgt caggaatgcg ggtcttgtgc ccgtgaccgc cagaacgttc gtgagaaggc 118440 agcgatgaag aaaacgctgg cgaacattct gtaacccaag ccgcccttcg gggcggtttt 118500 tctttttgaa acaggaggat aatgatgttc atctcccaag aaatgaaaaa ggcccttgac 118560 aaaaagcagg cgcatcggga taatggcgat cagttcaaat atcgaatcca gtacgacccg 118620 agagcttatt tctttaagta ccgggttcaa gagttcaggt attgcgaagg ctcttatgag 118680 tgttttgaac tcccggttca ttacaagggg tggaagacac tccgccgctt tacaaaatta 118740 agttcggcag agaagatcct gaacgaactt gagcgtgtga atggttactt ggcacctatg 118800 ccaaaatggt acagagagga gtgggagcac taatgattct gattaatttg aatgccccgg 118860 cgggcgcggg aaaagatgca ctgtgcgaag gtctccacag ttatatcaac accccggggt 118920 ttgaagtcgt ccacatggag ttcaaggaac ttctgttcga tatcgctgtc cgggcatccg 118980 gcctgagccg taagctgtgg ttcgctctgt atgagcgtga gtacaaagag aagccgtgcc 119040 cgtatctcca gatcaacggc gtaaacgtca gccctcgtga ctggatgatc cactgctccg 119100 agaacatcat gaagcccaca tttgggaatg atgtctttgg caaggcattt gccgccaagt 119160 tgcaaaagct gaaggatgaa accccggcag acgtgcaact ggtggttgtg gtcagcgacg 119220 ggggcttctt agaggagtcg attccggttg tgaatgtggt tggcccggac aactatttcc 119280 ttgtccgaat ccaccgtctg aaaaaagatg gctcggagta caatttcgat ggggattctc 119340 gccgatatat ctatgcgaaa gagtttcctg aacatctccg gatgtacgag actgatattc 119400 tgaacgaaga aggtgatctt aacggcacgg tccgtaagat ttgtgatttt gtgcaagact 119460 gtaaagggag cgacctgtat gtcattcact aagaccccga tcctggtaga tgtagatctg 119520 acgttcgttg attcagggtg gccttggctt tactggatgg aaagtgtcta tcaaatccct 119580 gcggacgttg atttgatgga caaggattat gaggaaaagg ggttgttcaa ttacaacctc 119640 tccaaatact tcccgactcg ttgcaaagag gcgattgagc cgtatgagtt ttgggaagat 119700 cctcatctgt acgacaagat gacgccgatt gccggggcag tggatgcaat tcgtcgtttg 119760 cacgaggccg ggcacccgat tcgtttcgta tcttactgca agaaggggca cttcagcagc 119820 aaggtacgat tcctgaagcg tcattgtccg ttccttgatc tcgacaacgg gacagacggc 119880 agcggttttt atgccacaaa ggttaaagct ggcgtcgctg gaggggtaat cattgacgat 119940 cggaaccagt tcctgaatca attcccggac gacgtgatta agatcaagtt cgacacgcct 120000 ttcagtcagg atgttgagcc acgcatcaat tacgacttaa cttcagcgga ctggaaagaa 120060 atcgctgatt ggttgctgga aaacctgtaa tgcgttgcgg aaaaggtcga tatgttgtcc 120120 gctacgggga gcatattgag gtaagaacgg agcagggaga cctgctcact tctttcaggg 120180 tgacgcagga tatgtgcaag gagatgcttg acttgtacct tgccgtcagg catgatgttg 120240 ttgttcctta caaaattcgt aagttgaagt aataaaagga gattttatgg gtcaggtatt 120300 tagtcgtggc aatatcgaag ttcagatcat cgccgattct gtggggcagc gcagtggtcg 120360 tatcacgact tttgaattga cttaccctcg catcatccac agtgagttca tgactcaccg 120420 actgttcagc cgcaacgcga tgtcatcccg cgccgtgccg atcaagaaga tggttgaatt 120480 gatccgtacc aacatggcaa tgcctgtccg attcggatca aaccaacccg ggatgcagga 120540 caaaggcgtc gagcacgacg gtcaggtttg gtacggttgg accgaagacg aagacggttc 120600 tatgaaagaa ctgtattgta ccggaagaga ggcatggcac cgtgcagcag aagaagcctg 120660 ccgatgggca gagcgctttg acgaagcagg gtatcacaaa cagatcgcca atcgcctgat 120720 cgaaccgttc cagatgatga agacggtcat gaccacaacg gagatggaaa acttcctgtg 120780 gctgcgtgtg gatgcggatg ctgacccaac gatcgaagtg ctggcaaacc tcatgaaaga 120840 ggcgctggaa aactccgagc ctgagtggct acagcctggc gtgtggcaca cgccgtatgt 120900 tgaccacgtg tacggcttcg ggccggaggg cgaggcaaac atcttcgaag gctattgcgt 120960 tctggatgag aatgatcgcc cggtaatgct aacggaggaa gaggcgctac gcatctctgc 121020 ctcttgttgt ggtcaggtga gctaccgccg tctggacagc accaaggaca aggcgctgga 121080 catctacacc cgactggttg ccgggaagaa ggtccatgct tctcctttcg agcatcaggc 121140 gcgtgagatc gaagagactt tggatgacgt cgagtacggc cagcagcaag tgaacgtctt 121200 ctggtgtatt gagacatggg aagaggggat tactcatgtt gatcgtgaag gtaacttctg 121260 gtcgggtaac ttcaaggggt tcatccagca ccgtcagtta atcccgggcc atgttgcaaa 121320 ataaagtttg acaaagaagt aaccttaccc tattctgtta tgaccggggc attggccccg 121380 gaatttgtgt acgaggagat gtagaatggc tttcggtaaa aatattaaat atggctctgt 121440 gactctggat ttcattgtcg agtgtgcaaa gtttcttgga aaggacatga agaaaaagga 121500 aactcttcgc caagtgttgc acctgatcgg atttgaggtc tctgtcctga atgaagagac 121560 tggtctgtac gtcccatgca agattgaccg catggacggc gtaaatgtcc gatgtgctga 121620 taagccttac atgttccgca cgacaaccat tttctcaggt cgtctgcgtg atgaacgtga 121680 cttcccgttc gtggggattt atgataaagt agatgtcctc gatgtaaaca gcgccgtggg 121740 tgctgcactg gtgaactccc tggcgttcga catcccatct gtggaaaagg tcaacacccg 121800 caagtacacc aaacgcgagg atcgtagcga cgttgttgtc atggacctgc cagagttcga 121860 cgacatggat gatatcttca acctggtgaa aggagcgtaa tcatggcaaa gaaaaacaat 121920 gctgttccgg acgagcagac taagtggttc aaacgtggct tccaaagcaa agccgagtac 121980 attggctggt tgcagttcaa cgacctgtgt gatgagtcga tgggttacga cgatacgtac 122040 cacgacccgt actccggtaa aacaatccgc ctcggtgcgg cttccgagga agatgttgag 122100 cagtacgaag tcaaaaagcc tgaagctaag taattcaata aggagatccg cttgaccagc 122160 attaatgttg taaaaaattc cggcgcgaca gagaagttcg atgccgagaa ggctaaccgt 122220 gtggtagagt gggcatgtga aggtctcgat gtgtgtgcat cagagatcct tatgaaagcc 122280 cagattcaaa tgttcgatgg agtaacaaca aaggccatcc acgaaaccct gattaaggcg 122340 gcatccgacc tgattagtgt tcagaatccg gattaccagt acgctgctgc acgactgctg 122400 ctgttcaaga tccgcaaaga ggcatatggt gatttccagc cgttcgatct gttccaccag 122460 atttatctga acgtgggcaa cggcaagtac gaccgccacc tgctggacga ttacacccgt 122520 gatgagattg ctttcctgaa tgacaagatc gttcacgacc gcgacttcga ttacacttat 122580 gcagcaatgc gtcagtacga agacaagtac ctggtcaagc accgcgtcac cggggagatt 122640 tatgaatccc ctcagatggc gctgatgctg attgcaatgg gcctgttgtc acaggaagac 122700 aaaaagaccc gtctggaacg tattgttgac ctgtacgaga acgtatcgct gatgaaggtg 122760 tctttaccga ccccgatcat ggcgggtgtt cgtaccccga cccgtcagtt tagctcttgc 122820 gtactgattg agtctggtga taatatcgac cagatcttta acgccaacac ggcgcagggt 122880 aaatatgcgg ctcaacgagc cgggatcggc ctgaacttcg gcatgattcg tggtcaggat 122940 tctgccattc gtggtggtga ggttaaacac gcaggcaccg tgccgattgt ccgcatgtac 123000 caagagtctc ttgggtggac ctctcagggc ggcgtccgta aggcatcggc taactatttc 123060 tacccgatgt ggcactggga ttttgagagc ctgatcgttc tgaagaacaa ccgtggcacc 123120 gagagcactc gtgctcgcca tgtggactac ggcctccaga tcaacaagct gctttatacc 123180 cgcctccagc agggcaagaa catcacgttg ttccatccga acgtggctgg cggtaagctg 123240 tacgaatatt tcttctctga ccaggagaaa ttccaacagc tttacgagga actggaagca 123300 gatccttccg tggcgaagaa gtctatccct gcggcggacg cattctctgt ctttgggcag 123360 gagcgttcac aaactggtcg tatttatgtt cagcacgttg accactgtaa taccaacagc 123420 ccgtttgacc ctaaacaggc cccggtgaag cagtccaacc tgtgtatgga gatcgcgctg 123480 ccgacgcatc cgatcaacga cattaacgat ccagaagacc cgggcgaggt agccctgtgt 123540 actctgtctg ccattaacct tggcaagatc gagaagctgg aagacatgcg tgagcctgtc 123600 cgtacactgg ttcgtgctct ggaccgactg ctggactatc agaactaccc ggttctggcg 123660 gcagagaaga ccaagctgcg ccgtacactg gggattggct ggaccaacct cgcgtactac 123720 ctggcgaagc gcgggatgaa gtacagcgac cctcgtgcag ctaacgctgt tcacgaactg 123780 gctgaagcat tccagtatta cctactggaa gcgtcggttg acctggcaaa agaactgggc 123840 aaatgtgatc tcttccacga gaccacttac tctcagggca ttctgccgat cgaccgttac 123900 aaaaaagagg tggatgctct ccacactgcg gaactgaaga tggactggga aaagctgcgt 123960 gtcgatatcg cgattcatgg cctgcgtaac tccacactgt ccacgatgat gccttgcgag 124020 acatccagcc agatcactaa ctccacgaat ggttttgagc cacctcgtga tgcgatttcc 124080 ttcaagaaat ccggagcggg tatgctgcca gtgttggtgc cgggggtagg tgacccaagt 124140 gtggtttacg agtacaagtg ggacatggct tcgcctattc cttacctgac tctcgtggca 124200 atcatgcaga agttcatcga ccaggcggca tcggcgaata cgttctacaa gccgtgggac 124260 ttcccgggcg gaaagctgcc gatcaaggtc gtgctgcgtg acattatgtg ggcgtataaa 124320 cacggactga agactctgta ttaccaagag accaaagacg gcaacgatca ggaagacgat 124380 ggttgcggcg gcggggcttg caaactgtaa ggggcagtat gactgtgtta tggtggttca 124440 tctatcgccg ctggcggatg atcagttgga ccaagacgta tgggccgttg gagaagaaga 124500 ctctcatcaa caacgcatac aaggtgaatc tgtgctcgtg tcgtgaccac aaagcgatct 124560 accgtgacat gcagaagatc ctcaaatagt aaaccgccct tcggggcggt ttttctgtct 124620 tagggtattg acacttggtc cggcatccgt ataatggacc acaagtttag agaaaaggag 124680 aaaacaatgt cttacagcac tttcaaccag aacaaattcg atttcctgac tcagccgatg 124740 ttcctcggcg agacaccgaa cgtttctcgc tatgaccagc agaaacacgc caagttcgag 124800 aagctgatcg agaagcaact ggcgttcttc tggcgtccgg aagaagttaa cgtgacccgt 124860 gaccgtatcg actacatgaa tctgccggat cacgagaagc acatcttcat cagcaacctg 124920 aagtaccaga tccttctgga ttctgtacag ggccgtgggc cgaacgcagt tctgctcccg 124980 atcgtgtccg acacaaccct ggaaacatgg gtcgagactt gggcgttcag tgagactatc 125040 cactctcgct catacaccca tatcatccgt aacatcctgg cagatccgtc gatcgtcttt 125100 gacgacatca tgaagtcgcc ggagattatc gcccgcgccg aggcggtgac gaagatgtat 125160 gatgacctgt acgaactgaa ctgccgcaag gccctgaacc ttccggttga cccgtacact 125220 catgcccgtg cgctgtacct gtgcctgcac aatatcaacg cactggaagc gatccgcttc 125280 tacgtgagct tcgcctgctc atttgcgttc ggcgagcgcg gggtaatgct gggcaacacc 125340 aacattattg aactgattgc ccgtgatgaa gccttgcatt tgtccggcac tcagtatatg 125400 ctgaaagaaa tgcagaacgg taacgaaggt gatctgtttg ctcgtgtggc agaggattgc 125460 tacgaagagg cacgacaggg ctttatcgac gcatcggtgc aggagaaaga ctggggcagc 125520 ttcctgttca aagacggttc gatgatcggt ctgaacaaag gtatgctgga cggttacatc 125580 gactacatca cagatcaacg tatgcgggcg gttggccttg gtgaactgat cgggaaaccg 125640 accaagaacc caatcccttg gatcaacagc cacctgaata ccgggaatgt gcaggtaaca 125700 ccgcaggaaa ccgagaacgg tcagtatctg tccggccagg ttgattcctc tatgtctgcc 125760 gaagatttca atatggacct gtaaggaggt tttatggcaa agctgaaatg taagaccgtt 125820 ggtaatgggt atggaaagtt cttcaccatc ggaaaggtgt acggggcaga tttggagtat 125880 ggtgttgctg acaacgactc catcgacgag actgatcaca actgggaatt ggagccggat 125940 ggggttacgg tccgtgccgc ttgttgcgat gaagacagcg ccattgtggc aacttttgaa 126000 attgtggagg aatgatggaa gacctgcaag atttgtattg caaggtggtt gccaaatcta 126060 tgaacaaccc gttcaaggct ggctcggtgt acaaatacag caaagatctg aatctggtgt 126120 atgatgctga gggtatacct catacgctgg gcacttttga aaatcacggc ccgctggagg 126180 cacacgtcga ttatttcgac gccaagtctc cggtactggc tatgtttgtg gaggtttgat 126240 gtactttacg gtcttcggta aggaaaactg tccgtactgt gtacgggcca aggagcttct 126300 ccagtcaaaa ctgttggagt ttacatacaa ggatgtttta gaggacgaag tgttctttat 126360 gcaaatgaac gcttgggtga aagatgcgac cggacgacct gctgcaactg tgccgcagat 126420 tttccttgac gatcggtaca tcggtgggta cactgactta gtggctcatt ttgagtctca 126480 aaaagaggtc gttaaagctg acgacttcga tattggcgaa ctgtaaggag aagggaatga 126540 cagcactggc tgagaacatc gtggtttccc cggaagaggc ggaactggac attctccatg 126600 acgtggctat tcgcctcgcg gagatcaaca tcgaaccgca gattgccgta gctgtcgata 126660 ctctggagtt tctccaggat attgaagaca aaacggctga tgaccttcgc cgcatcaaag 126720 atcaagagaa acttattgat cagttgtcag gcgaagatta tcgtaaccgt ctccagaatc 126780 tgttcgctga acacttggcg acactggagc cagagcacct gaagcaatat cacgcagata 126840 tcgtctatca agatgcgatt gcagaaatga acctgcaact cgtcccggcg cttcaagaag 126900 tgcagcagga gcttctttat ggcgtcgtca gtaagccgac cctgcaataa ggagttgtag 126960 tgccagcacg atttagtaaa gaaggcatta tccgcgccaa ggtgaatatc gaggcagggt 127020 atgtcaacaa caagaacgac cttggtggtg aaaccaatca tgggatcacc attgcaacgg 127080 caagggagtt cgggtataaa ggcgcgatga aagacttgac agtggcccaa gccttggaca 127140 tttatgaccg tggttggtgg cagcgtatgt ggttggatga tatcttggct atcagcccgg 127200 ctatcgccga ccgcctgttt gacttcggga tcaactccgg acgtgcaaac gcagtcaaga 127260 ctcttcagcg tatgttgaac gtactgaatc gccagggcaa actctacgga gatattgcca 127320 cggatggagg catgggtaaa aatacccttg ctgccctgaa ggcgttccag aaggcgcgtg 127380 gagacaaggg catcaacgtt cttgcattcg cactgacctg tcatcaggtg tcttattaca 127440 cagacatcag tgagaagcgt caggccaacg aggagttcac ctacgggtgg tacgaccgtg 127500 tgttccggga aatgccggag tacaacgttg aactgggcct ttgccagaag taactgacgg 127560 cccccgccgg gggccataaa cagctttcgg aggtactatg cgtcataaca atcgtcaaga 127620 gcgtcaatct cgtcgtgaga agaaggcaag tcgtgagcgt gtggtaacac cccgcgcccg 127680 cgaggaagtt gaactggcta aggctatcca gacccggacc cacttccaga aaagcctttt 127740 gagcgcggta aaccagaagg atgccgtttt cgtaaacggc cccgctggca ctggtaagac 127800 tttcgttgtg atgagtaccg tcattgactg gctcaaatct gggaagatta aaaagatcgt 127860 cctgtcccgc ccaaccgtgg gtatggggaa cagtttgggc ctgctgccgg gtgatatcag 127920 agagaaattt gaaccgtacc tcgcggcact ggttcaggtg atcaaagatc gctacggggc 127980 gaactactac gagacccagt tgaacaacaa gaacatcgag ttcgttcctc tggagtacgt 128040 gcgtggtcga tcctttgagg attctgtggt tattatcgac gagtttcaga acacggacga 128100 agagaccgca tatacgatca tgacacgact gggtgaaggt tccaagatgt tctgcctggg 128160 tgacattacc cagaacgaca tgaagggccg tgaatccggg ctggactggg cgattgagtt 128220 tatcgatcgt cacaacctct tccatctggc agagtttgtc gaaggttcca gtgacgatat 128280 cgtccgtagc ccgttctgta aggcagtggt taaggcgcgt gagcgtgact tgaaggagca 128340 gtaatgacgg catacaccgg gattggttcc cgtgaaaccc cggctgaagt cgtaggcgtc 128400 atggaagacg ccgcttaccg actagcccgt atggggtttg ttttgcgttc cggtaaagct 128460 gccggggcgg atgcggcgtt ccaacgtgga gcgcagagat ttgaaggtgc aaaatgtgag 128520 atcttcatcc cttggagagg tttcacaggt ggtgatggcc ttgtcaatac gtgggacatc 128580 actttggacg ctgtggacag acagttcccg gaacacgcag agatgcgctg ggagtgggtg 128640 aaggaagtgc atggtggctg ggagaggttg tcccaaggtg ctcgtaagtt gcatgaacgg 128700 aacgtccacc agttgtttgg tgcggatctg ggcaatgcgt acttgaacca gagtaaattt 128760 gtgctatatt acgccctcga aaccaagaag ggtgacccga aaggtgggac ggcgacggca 128820 gtgaatcttg cgaagaagca gggtattcgt accttgaacc tccttcatga gaaaaaccac 128880 gaagtccttg agaagttttt ggtaagtatg gaggaaaagc gtggccttaa atcgtaataa 128940 ccttaacagc gcttttgcac tgggtaagga taagaagacc gggaagtggt cggtaggtca 129000 gtgggccaac caagcgctct ggaccgtgga tgggtccaac aagcgtatct gtgctttcaa 129060 ggagatgacc cattggcgtt atttccgaga ggcaattgag cacatttctg atgtgcagtt 129120 gtcccctggt gatcgggttg gcattgctgt taagcgaggt atcaatgagt ggtactacgt 129180 tattgacaaa attacgtcag atgacggaaa atttcactac ctcgtcggta tctaagggag 129240 agtttatgga taagggtatt tttgccacag ttgaacatcg caaactgatg gaacttaaaa 129300 actgtggtgc tgtcgatgcc gctcgcctgg aagatctggc ggaactcctt ttcttggaca 129360 tgaaagagga aatcatcaac gtccgtaagc tacgggttaa gcccggcttt tgggccaaac 129420 tattcgacct gtcgatgcca gttgagcaag aggtccacct catgttctca aagcgtcaag 129480 aactggttga ccatttggca gatatcgagg tgccaagtga actgtgtccg gtgacaggcg 129540 atgcaaaaga gcgcctgctg tttatcgacc gggcggcgcg gtatgcggtc gaggctcaaa 129600 agatcctcga tatgttcaag cgccatcatg aagtgattgt ggattcggat cggtctcagg 129660 ttattgagtg gattctgcaa aaccacatcc gtgtccgtac tgaactgagc aagggggcct 129720 gatggaagag caattcgata ccgttgatca aacttaccgt gggaatctgt ggtgtactcc 129780 ccaagagcgt cgtgactggc gtctctatgt gaccgaactg gacaatgatc cacgaaacca 129840 cttcgagggt ttggacatga ttcggaccgc agaggaagag gatgttattg aaatcctgat 129900 cacatcaccg ggcgggcgag tagacattgc cgacatgtac ctcgctgctt tccgtgacag 129960 caaggcgaag atcattaccc gggccattgg tgagtgtgcc agtgcagcga ccacgatctt 130020 cctggccggg gatgagcgtg tttgtgaaga cggctgctat ttcatgttcc acaacgttca 130080 gttgggatct ggaggtgata gtgcccacgt gatttcccgc agcaagtttt atgaacgtct 130140 ctttaaagag aagttctacg ggatgatggc ggaggttctg acacctgtag aactgggtga 130200 gttgttcgaa cgtgcaggcg aaatctacct gacagcggag gagatgcgtg aaaggctgga 130260 taaggccgcg cctaatcaac ctgaaatcgc cattgtcaat gagaatggaa agtccggggt 130320 tttgatgaag acccctgacg gagggttggt agggtatggc gcaactacct gcgaagaggc 130380 tcgtggtgaa ctctcccccg gggagtggtg gaatttggta accaagggac aaaacaagcc 130440 gacgaccgag gttgtgtcga tgcctttccc agagaccaag gttaagtcgg cggaagagtt 130500 cccgcaggga gacgagttcg acatcactct ggacgacggc tacaaaaagg tgtttcgtct 130560 tagtaccctg tgccagaagg atttcgatga gtacaacatg gacgagatcg aggagatcgg 130620 cattgccttt ggccttgatc tcgatccctc gcagaatact cgtcagcagg cactggagtg 130680 cttgattgaa gaaatcctgg cgggaagtca gttctatgtt ggagaggaag attaatggcg 130740 gcactgaagc cgaagggcga acgtaaggca ttcgatgtat acgaaactcc tgagtgggca 130800 gtaaaatgtc tgttcgacct gattccaatc cgcaagggct ggacttacat ggaaccttgt 130860 cgcgcctccg ggcgctttta caacgaactg ccgatcgggt cagcgtgggg agagatcagg 130920 gacggtgttg attaccttaa tacttcttac gatcgtgtgg attgcattat caccaatccg 130980 ccctactcct tggcccaaga gtttgtggag aaggcccata aagatgcaga tgtcgtaatc 131040 atgctgctgc gcctggggtt cttggagagt atgcgtcgag aggagttctg gaaacagtac 131100 ccccttgacc acctgatcac gttgtcgaag cgaccatcct tcaccgagga tggtaaaaca 131160 gacggtgccg ggtacgcata tttcatctgg gacccactga aaaagctgaa tctggggaag 131220 cctttctact gggtctctcc caaataacaa aaaggtcacc ttcgggtggc cttttttatt 131280 gcattgcgac cagacatgtg tataatgacc atcattgaag aggagataat catgaaatac 131340 gacatttttt accgcccggt gtttacaaag aaagccaaaa ctctgtcagg gtatgaaatg 131400 gtcgtgtacc ctcatgatcc tgctcgtcca cacgatgatt ttgacgtaat gaccattgag 131460 gcaaaggccg acaatgagtt cactcgattc gaaaacatgc ttcgtggtga gtatgacccg 131520 ttcagcgaca tttcttttgt tgacaagcac ccgctgaata tcataagata cctgcgtggt 131580 gagaaagaga tgcccaaacg aacgatggat tgggaaaagg atattgaccg ctggtctgtc 131640 aacttacgag gagaaagagt atgagcggat acgtagaaag aacctacctg tttgaaggcg 131700 ggacggtgcc gggcaagttt ttcgatgatg tgtacaaccc atcatggggt gccaagcacc 131760 tcaaggttgt tgagtctgga gtcaccaact ggggtgatga tacggaagta gtggtctctg 131820 ccgattgcga agagacattc gatgattatc tgcacaactg ggaagggaac atcaaaaaat 131880 gaaaatctat ctggtcggcg gcgcggtccg cgatgaactg atgggtgttg agagcaaaga 131940 ccgtgactac gttgtggtcg gctccaccca cgaggagatg atggcactcg gcttcgagca 132000 agtcggtgcc gcattcccag tttacctgca ccctgagacc aaggaagagt acgccctagc 132060 ccgtaccgag cgttcaactg gcgcaggata ccacgatttc gaggtgatgt tcagcccaga 132120 tgtgaccatc gaagaggatt tggcccgccg ggacttgacc attaatgcga tcgcaaagga 132180 tctggagacc ggggaataca tcgacccata cggcggtatc cgtgacatcc aaaaaggtgt 132240 tctgaggaac gtatcagcgg cagctatgcg tgaagacccg ctccgtatct accgcctggc 132300 tcggttcttg gcccgctttg gcggcaagtt tgtggtggac cactcaacga tgttcatttg 132360 tgagagtgca aaagcgacac tggctgcgct cccgaaagag cgcaagatgg cggagatccg 132420 caaatgcttc gaggaccaga gtaatgacaa ccgaccctcg ttgatgatcg atatgctctg 132480 caatcttggg gaaatgccgg aggtggcaaa cctgcggggt gcattgcagc cgaaagccca 132540 ccaccccgag ggtgacgcat acgtccacac gctactgtgt ttggactatg cccagcgcat 132600 catgtcatca ccggaggtga aatgggcagt cctgtgtcac gacttgggaa aggtccagta 132660 ttttgagtac gggaacctgc acggacacga agagtttggc cttgggttgg ttgcacaact 132720 gagcgagcga ttcggcgtac caaacaaatg ggcagacctc gcccatcgcg tttgtgccga 132780 ccacacacga ctgcaccgga tcatggatct gaagcctcgc aaggtattcg acctgattga 132840 acgcatcaag gcgcacaaac cgcaggaaaa tcagttcgca ctgaagttca ttgaggcttg 132900 tatgtgcgac gcacggggcc gtgggccgga gaaaatgttt gacatctacg accagcctca 132960 tgtactatgg gcctgtgctg ttgagttgca caagaaccga ctggaaatct ccgagaagag 133020 taaagagatc tccacgagat tggcaggtcg tcgtgaatta atctcttctg agatccgagc 133080 gatgaaagtt aaatacgttg cccgcgctct atcacgagta aaggcagaaa tcaaaaaatt 133140 aagaggggag gaagggtatg acgattccaa tttacctact tggcgtctgt attagtgcag 133200 cagtgtggtt cgggattatc aagtggcggc agactggccg ggggataaag atgccggacg 133260 ccttcgatgg gtggtatctt tgccctttgg acaagggtaa aaccgtggat tggtccatgc 133320 taatgctggg gtgggtcttg atcgcagtgg tttggcccat tgcatggttt atcctgatca 133380 gcggttacgc tatcgcgtgg gcaattgaaa taatccgatg ggtttggaac cacacgatcg 133440 gcgatgagga attggcccgt aagattttcg gaggtaaaga gtgaagactc gtatgtattt 133500 cttggtcaac cagtatatca tgggtatcca ggcaggtatc cagccgggac atgctgcttg 133560 cgaactgctg gctgactact gggacgaacc gcgagacaat ccgaaggtaa agctgatcga 133620 gcaatggctc aagaaggata aaacctttat cgtgctggac ggcggatatc agcaacggat 133680 gtatgaattt gcggataaga tcccaagttc cattccgttt gcctacttcc acgaggaaga 133740 agatgcactg aacggagcat tgaccgcagt ggcctttgtt ctcccggatc acttctacga 133800 cgtgaagttg gatgaggacg ggaaggcccc ggcggggtac tttgccgagg gcgattactg 133860 ccgctgggta gactttacgg aaacagaacg taatttcatc gtcgaactga aaaagttcca 133920 tcttaaaagg ggttaatatg tttgatttaa tggttgcggc ctcgaacgcc atcggtagtg 133980 tcgtgatgat cgggtgggta tgggcgataa tcggtgtcct ggtgtggtta ggggtggtac 134040 tcttggccct cgccggggaa ccgagttaca atcgtgaaaa tggggccgta ctggggtcat 134100 tggttggtgc cgttcttggc ttcatcaccc cggtactcct gacaatacta ccgttctttt 134160 tacctttcat cttggcggcg gcaattctgt ccctgattgg gctggccttg gcacatttct 134220 gtaaggttgg caaatgataa aagttaaaga gttgattgag cagttgcaga agtgcaaccc 134280 ggaagacaaa gtggcggtac agatcgacaa aggtttcgtg accattggcg gaacaccccg 134340 tgtcggtgta gactctgccc acagcggatt cgactgggac cacgggactg tctttctgaa 134400 actggaagac ggaaagatgt tgacagacat cacccgggaa gagtattatg agcacatccg 134460 gtacaagcag ttggtcagtg ggatgcgtgg caagcaggac attgcccact tgtcggatgt 134520 gtttgtgaag aaggacttcg ttatcgagcg actgaagcgg ttgattaacg ttgaagagtt 134580 agaacctttc gaggtctctg acctcatcac tgatatttac gaggagaaat tataatgttg 134640 gcaccaactt ggcagtacat cgttcttatg gtaatcttct gcttgggcgc gagcctgaat 134700 gtaatcagct tgggcctgat gctcttcttg gaaagcatta agccggaggc gttgaagttc 134760 gaacagcgaa cgttaggtcc cgggtctctt ctcttcaaga ctttgatggg tatctttgca 134820 gcgtaccttt cgtactcgct gtacatttgg tcccctggcg cactgagcct cggcatcatg 134880 attgtacact gggcatcgat catcgttagc tatatcttgg gcattgccaa tgcaggcaag 134940 gcgaaaacgt acacttacgg tcagcacgtc ttcggtgcaa tctacacaac cgggatggtg 135000 atcgctctga ttttctacgg ggtaaaatgt ctgtaacaaa agactgtact aactgtgaca 135060 gtttcatccg ggtgaaaaag cccggatggg ttggtctctg tgatttgcac gactggagga 135120 ttaatggaaa ataccattgt gacaaatgga agggtaaaaa gcatcgtcga gaagctggaa 135180 agtcacaaag acgtatcgaa ggtgctgatg ctatgtctcg caggatctca tgcgtatggg 135240 acggcgacgc cgactagcga cactgatatt cgtggcatca tcgttgcccg cccgggagta 135300 atccgggcac ctttccgaac cctccgtgag atggggatcg aggacgagga agatggaaag 135360 gtttacgaac tgaacaactt catggagttg ttctgcgaca tgaacccaaa tatcattgaa 135420 ctgggcttca ctgatgatca ggcgactctg tacaagcatc cggcgtggga ctatttgaag 135480 tccttcctgc cgcaactgct gaacaaaaac gtggcctacc gcttcggcgg ttatgcgatg 135540 gcgcagttga aacgcatcaa gggtcacaac aagcacatca acaacccgat gccggaagaa 135600 aagccgactc aacttgagtt cttccgtttg gtacagagct acctcgacgt gaaggtgctg 135660 aagcacgaag acttcatgcg taagctgaac aatatgcagg gtgtctgcac gttggtgccg 135720 ttcggggcgg acatctatgg cgtagtaccg gaccaccgtg aagatgttgg gccgatgttc 135780 aaccccgacg ggtcgatccg taaattgcag tacgatgaga tcccagagtc tgtgaaaaag 135840 cagccgccgt tgttcatagt taaatatctt cgtcaggatc acacggttgc caaggataag 135900 caccacaact actggcagtg ggtgaagaac cgtaacgccg cccggcatga gctggaggag 135960 aagttcggtt acgacaccaa acacgccatg cacttggtgc gcctgctccg tatgggacag 136020 gaaatccttg aaacggggca ggtcatcgtt cgccgcccgg acgcagagga actgctgggc 136080 attcgtaacg gaacccacag ttacgaggac atcgttgagt gggcatcgga caaggacaaa 136140 ctgatccgtg aagttctcta ccacgagagt aagctgccga agaacagcaa caagcgattg 136200 gcggaagaac tgatcctcaa tgtacaggat tctgcatggt cacaatgact aacgggagaa 136260 tggcgcatct cctgtctgtc gatacacacc gggccaaaag cccggttatc tcttggatag 136320 aggaaaacat tcgcacaggg aaggcaaatc ctgattacgg atcaagttgc acagaggtaa 136380 tccaagaggt ttcgatcagg ctggttgaga gcgtggatta tgaggtaaag gacggtaagg 136440 ttatccatgt cattactgga tggaaatcat acccgtattt tgagaatccg gcaaaaccga 136500 caaaacaaga gattgacttg ttggaggtat gcgagcataa tcactttata tggttgagag 136560 gactttgttt tgagatcctc gatatgctgg accatccaca ctcatgttcc atcgactacc 136620 acattatgga agaggggtgc agcgggtcag ggtgtgagag agcagggcca gtaaactacc 136680 acgggggtag aagctggcaa tattattgtg gtggagggcc gcgctgctgc ccataggagg 136740 agaaagtgca agataaacct ggttatcatc tggacaaaat agaaaagcgc cgtttcggat 136800 ctctcggcaa gattcaagag gaggtcgagg aactggtcga tgcacaccgt cagggcagtc 136860 acgtcatgat tcttgtcgag atgtctgatc tttacggggc catgcaagga ttcttggagg 136920 aaaactaccc ggggttcaag atggaagacc tgaaaaagtt ctccggcatt acccgccgag 136980 cattcgaaaa cgggcacagg gggtaatatg aaatatccta tgaacttggc actggtacgt 137040 gacgcgacta actggtgggg gctttggttt gagaccaaaa aggtcccggc ccttgtagac 137100 ccggatgtta tgtctcacac ctgcccgccg ggcgtacatg attcccgcct gtacggtaaa 137160 tatccggata ctgttgttgc ctcggcggag caatcttttc tccagttgga gaaagagggc 137220 aaacttaaac cggggcgttg gatggctttg actccgtgct accgggatga accaattctt 137280 gatgaaactc acctgcctgt gttcctgaaa ctggaactca tgaagctgag tgacgacagc 137340 aacttctaca cccggacgga tgcactgtgg atcgctggga agatgcagaa gtttctgagg 137400 gagttttatg cactgcctac agaggtgatc gagacagagg acgggttcga cgtcatgtac 137460 gaagatctgg aactcggatc atacggtgtg cgtaaaacca tgaccggaaa atcgtatgtt 137520 tacgggaccg gattggcgga gccaaggacc tcgatcgcat tggaacgttt ccagcataaa 137580 tgtgaaacga cttttgacta aaaattcatt gcaaggttat gcctattgac cagatcccga 137640 cgataggcat tttcgatgta ttaaaataga ttttagcaat ttaaatgtga agcggagaat 137700 ttgttgtgta aaatgacaac atggcaactt tgtcaagagt cttctgttgt atttttgcat 137760 aacatggtaa aatggttgtg cggaatctta caacgggagg aaggctatgt tttacgcata 137820 cgttcgtcaa cttccgatgg tagagggtaa cctcgatctg atccgagggg atcggttgga 137880 ttactcgtgc ggatctatcc gccgggccaa ggccgatctt tggggattca gggcatacgt 137940 ccttgaagat aaggtccgag gggttactta ccgggtaaat cagcgcggca tggttgtgga 138000 aacaccatct gggtcgattg cagtcccgaa ccccggagag ttgaagcaca ctccattcgc 138060 tatgacaggc gacgccggag agatcaatgc tttcctgaca ctctgcaaga atcttgttgt 138120 ggagcataag aaaagaaact ccctgttcgc tcgtctaaag cgcttcacac gcaagccatt 138180 cggaatgatt ctcattgccg gggcggcggt atacgccatt gtgacagtca tcgtgctgtt 138240 ctttggataa tcgggtcgat cgtcgaaaga cggtcggcct tttcttttgg agggtgcatg 138300 gcaaatttta acactggtaa tgtatctaac ccgtattcaa cgtctcccgg ccttttcccc 138360 gcttcgacac aaaatatggt gatacaaggg tcattgcaat cagtgaccaa tctacctttg 138420 acggcaaacc acggcgacac ctatatcatc gggggtgttc tgtatatgtg gatgcacggg 138480 agttgggcaa acttaggtgc gccgggagga ccgatgggtc acagcggatc atacgaaccg 138540 ccgaaagaaa ttatcgaatc caagttgatc tggaagggta tttcggttac attgtattcg 138600 gaagaggaca taaaggctct cttctttaac cagtatgccc tcaccaggga cgatatggca 138660 ttcttccaga tgacgtatcc ggatgaattt aaagcgttta gggagcagtg ggcggaaaag 138720 tgggccatcg aaagggccaa agaagaattt gacgaatggg agttagacta ctatgagaaa 138780 aagccaagaa gaaattaccc gtgaggtatt tggacggggt gctcgctgga tcgggtggaa 138840 ccactggctg aagcaccaag ttttcttcaa gttccttgaa catcgggtgg agagagggat 138900 agatatagac agtcgggcgt cgttctacct caaaaccaag atggattctt cttgggagac 138960 ggacaagatg gtaaaggcga atatcgatcc tgagacagga gatctggcga gcatagagat 139020 ccctggtatt gggataaaag gtctccgggc cagattggta gtcactgaaa actcctgcac 139080 ttacgagtgt gacggtgtaa agactcattt atcggagatg tttgacgacc cttggcgtcc 139140 gacatttgaa gagttggata tgttccagat gctttacggc gacgagcaaa tgctcataga 139200 tatgaatcgg gagttcttcg gcgtcacccg aaaactcgat aaggcggcgg gttccaatac 139260 cctgtctgta tttttcccaa tgaaggataa ataatgagtg aaatcaaaga tgtaattgag 139320 agactggctc gggattggag ttggaaggcc gttatggatg accctgttcg acgtcagata 139380 gttactaagt tggaaggtaa gttccgaggt ttgactatcg aagtccccac cgggccgaac 139440 actgtccggg aatttaccat gcgccacgaa atgaagaacg gggacctgcg tctgtggacc 139500 aagagcatga gtgggcttct tcagtggaga acctgccctg agaagggtga tcgtttggtt 139560 tatcatgcct ttgggcgagg agataccgaa cgtctgtcta ttgaagaaat cttcttagac 139620 acgcacaacg tggacgaagg agaggtggcg atgttcgaga tgctgtacgc agatctttcc 139680 ccaatggtgc ctctctttgc tggggctttg gacgaagccg ataaactgtt gctggagaaa 139740 ttcaatgggc caaaagcgaa gcgctaatga tgcactgagg gcggcgcagt tgaacgaact 139800 gcgttacgac gcaactaccg gggttgtgat gggggtaaat gggatgttgg gaccaagagg 139860 ggatatgggg gtgtccgggg tgtgcgggac acctgggcct atgggtatct gcacagagct 139920 ttatgtttta tcgtatgcgg ggcttccctt taaggtccgg ttgaaaccag aaacctttgc 139980 catgattgag gatgctaccc aattgaacgg ggacgaactt tcttttatca aggtggtgga 140040 tgagtccgag tggaaatcga tcagggaggc ttgggcagaa gagtggaaca aacgccaagc 140100 gtaccgggcc tttgatttgg gggagatgat gcctgatgaa taacttaaat aatcatatcg 140160 acgcagcact gatgggtagc gaaacaacag gggtcaacgt gaacccgtgg agaattggag 140220 atggctacac cacgccgaac attcaccaac cttggactcc ctccccgtcc gatattaagg 140280 cccgggagtg ggaaaggatt atgagggaga tcgtgaacgc cccgacacca accaccactt 140340 cgaccgggac caaggttatc gtagaccctc ctacgtcgag aaacaggacg attaagttca 140400 aagagatcgt ctttacggct tacctttctg atgaggctat ggagttcttc gaggacgaca 140460 ggcacgtaac tcgggacgag cttcgattac tggaagttgc tcatcctaaa gagtacgcca 140520 agataaagca ggcatggatt gccgcctgga aagagcttca ggcactaaat gcttttgaaa 140580 acgattgaca ttgacacagg accctgtatg atgggtccta tcaaacaaca gaggagagaa 140640 gagtgaaaaa ggtatccacc atcgaaaaag catttatcat tggcgtgaca tccctgattg 140700 gcgggtttgg ggcatacttg ggccagatga ccacaccaca ggcggtagtg aatggcatag 140760 tctttgggat cttgggcgct gccatcatcg gccttctgca taagagtcgt agctgatgag 140820 caagaacacc cgatactctc accttttgtt ccacatcatg tcgcctgaac tccgtgagca 140880 gttcttcacc gacgatgaag agaacttcga tgcaggctgg gacctctttg agaccctgca 140940 tccggaaaag gccgaggtgc tggtcagttt actcgaaccg catctcgaat atgtgataaa 141000 agagttgaag ttccagcggg accataatat attgctgggc aaaggcgacg aactcggcgc 141060 tgcacgactg gcaatctgcc accgcgctga taaactggac tggtaaagga gatcaatatg 141120 ttacagatga acaaagaagt taccgctcaa gccgttacgg cgatgtctga gcctccggcg 141180 gcaaccggac tatttctggt gttcgcatcg gaaaaatact acccgcaggg cgggtttaac 141240 gacttcctgt gtgtggcact gtcgttggag gaagcccgcc gggtgatcct tgagactgac 141300 atctacttgg ggagctacca gatcgtgaac tacaaaaatc tggaagtggt cgaagctaaa 141360 gaaatcaaca ggttgaactg atatgagcag atctcgtttt ggtgttgtgt ctctgatggt 141420 ggttacgccg gggttcatgt ccttccaagg ctcaaactgc ggatttgccc agaactgcaa 141480 gatgatgttg cagacagcct tccccggcct gccgtatgac gtgttcaagc gtccggacat 141540 catgcagatg atggatcagg tggacaatgt taccgggacg ctgtatatca tcgaccctgt 141600 ggagggccag ccgcttatgg ctaatgcgac ccttgagggt ctggaagagg agtaccgcca 141660 gtgggaagag cgtcgttaat tttactggca ctcttggcct ttggggccag tgccgaggaa 141720 gacctgaaga agtgtaccgt gcatgaccac ggcggtggtg ccactatcat cgcctgcccg 141780 gattacgtca tcacgaagac atcagataag acgatgatct gtcgtcttat ggaaggtagt 141840 ccaaatgtga gttgcaccaa ggttccttaa aacgccctgt aacgcgctgt aagcgtttta 141900 gaggtgaggt aaggtgattt atcatccttg gcgagaaaac gcctcagaat cgctctggtg 141960 cgtcctgacg ggcattcccg tctaaaactt tacaggagaa tacaaatgaa ggtaaaaggt 142020 gccctttgga aacaattcta caatgacgag gggttctggg gcagtcgttg gcatgacgac 142080 accctgatcc tatttgacgg tgttgagcaa gaggagtatg acaacccagc agatgacgcc 142140 gtggttgacg tacaggccgg ggatgtgtat tttgaagccg aaggcttcca acttgccgcc 142200 cgtgatactt ctctggaggc tttctatcgc cgttggctca aaatccaaac taccgaggtc 142260 cttatcgtaa ctgtggaaaa atcccaagct gcatcggtaa aggcggaaat taaaaatatt 142320 actggcgtaa agggggtcaa atgattgatt tcctgatcct tgctacactc tggttctttg 142380 gtattatcgt accgctgggt ggtctgatcc tgatcgtaag gaaggtcatc gtcccgtatt 142440 gggtaaaggg ttggtccggt cagtgggtat tctggtacat actgggggct gttcccctgt 142500 attactacat cttctgcaag gtcttggtat ggataggggt ggagagtgga gcaggagctt 142560 aaaagaacaa ctgacttgat tggtgccttg caggctgcta tccgtgtcgc cgggtatgat 142620 atggactgcg gagatatctg gtcccacata aagggtatcg ctgctgcgga aataaagaac 142680 caccctggcc ctatggggcc tatgggcatg gctggtgccc cgggagagcc ggggaaggat 142740 gcgtgggata agtatgccta tgaccctcag accgaaagaa aagcccagtt cttctggaag 142800 ggtattgagt tttggtctcc ggtgccgcgc cacttctacg acgccttgcc tgtagggaag 142860 gagttgacat atggcgatat tggcttcatc cgtacaatgt acgggaacca aatcgtcgaa 142920 actatggctg tcttttggcg tcgtgaatac gacctgaaga cagatatgtc tttgtttatt 142980 aacgaggaag ataaatgtca tttagcgaat atcaacaaat ccgattcatc tccggcccat 143040 ttgaaggtct gatgggaaaa actctcgctc cggttgctga caccgaaaac tggtacgttg 143100 aggtgaagat cggtggtcgg cctgttgttc aggaagttcc tgagtcggac atcgcccctc 143160 tttaaaaaca accgccttcg ggcggttttt tattgcttgc aatccgatga tgttcgtgta 143220 tactggccct ggaggtgact tatgaagaca acgccgtgga gaaaacccgt cgcggagtgc 143280 ggtgggataa cggatctgat taaaagggag attaagttca gattccccag cggcatggag 143340 aggtataggg cagatatccg tatccatgct taccgggagg tagggacgag cttggaggcc 143400 agttatgagg ttgaggttga tggaccgttt ggtaaatttt tcaagtggga gaccatagtt 143460 atatgataca cgtggacgat atgcactggc acctgtggga ggccattgtt cttcaggccc 143520 gggatatgta ttacaaaggt ttcgtgagta aggaactgaa cgcagaggtc acagctatca 143580 gttgtgagac tgttgctaca gggaagtttt ctgaagacct gcggaccaga gcctgccttg 143640 tggctcaggc actggaacca aacgcagaag aatggcaaaa agccgtggct attgaatacg 143700 aggggatgaa tcgtgaagct gaaatgtatg aacgtgatca cagacttcaa caaggtgttt 143760 ccgaagggac aggtcttcac cgcttcagag cttaaaaacg ggttctgtga tgttgaaggg 143820 acccggccca agcgtgacgg ctctccgtgg acaggtgtcc tgtctctggg tcacgtggtg 143880 gtactgggtg ttgccaaatt cgagatcctt ccggaggagg aaagtgatga ttccacggat 143940 gactaagacc actctgcaca aactgtttaa agagtggaca tggcgagacc tgactcggcg 144000 taaatacgtc gggaacctga tctttacagg aaatggcggc taccgcaagg acaatcgtgt 144060 ggttcagaac gtcggcaaac ctgccattaa gtttcgtttc gatattggtc cataccttgg 144120 tgggacagtc acctccactc cgagacactt cagtatttcc acaaccggaa agctggatcg 144180 ggtctcttgg atggcaatgc ctgacgggga tgttttcttg aagcgtcgtg tcggaaaggt 144240 atggcaatcc ccggtgaaga tgacagactt cttcttggat tgccgtgaca tccagaacga 144300 agaactggag atgctggagt ttctgtatcc gggcataacg gctcaggtag tggagttgct 144360 gcccggcttg cacgggatag actctgcact ttgggaaaaa ttctgtggaa aggggccata 144420 tcatggaatg tctgcctaag tgttacgatt ctccacgggt gccgtcccag tggaaaaagg 144480 atcagcgtgt acgtatcttg cgcgggaaga ataagggccg cacggccctc gtttcctacg 144540 agacagagcc gcatcctctg tcaagtaagc gtcggttcta cctgtggctt gagtccaggc 144600 acaatttctg ggcaccgaac cacgtactga tgaacgagga tgggtttgtc gcagtctaac 144660 aaaagacctc cgtacaatgg gctacttgag tatggcgaat ttgagtacgg aggtcacaaa 144720 gtgagtctca ggttgcccaa agatgtgtgg gattatataa aacaggttgg agaaaaggac 144780 tttttatggt ccccggagga agaggtgttc ttcgaaatgc tctaccctgg ctggattgga 144840 tacattcggc atcaggttga ggttcaatat tcgtttttgg tgtttcaaac cagcaccttc 144900 ggagaatccc tgcttggggc cagagaaatc aaggattacg tccctgacga aatttcaacc 144960 acaagatatg gttgatttct accctatttt catgtattct gcacaaatcg ggtgaaaatg 145020 tgccgttttt gcatgtaaaa gtcaaaattc tggcactttt ctgcacaaaa ccgcaaaata 145080 attgcactca ggaggcaaaa tgatcgaaaa atctgcaatt gaattgttgc ttgaaatggc 145140 aaagactaac gaaaccctcc gccggaatat cggcaatttg atgatgttcg ggtattggga 145200 gtcgaaatat tacaattttg aggacatgat caaggacgag ttccccgaac tcctccggct 145260 gtacacacct tgggagtacg acatgcactg catgggtgtt tttagggccg cagccaacaa 145320 ggcgcagctt gctggtcttc cggggaaggt ggacatgttc aagactctta tcaatgccca 145380 caaggacagc atcaaggcta ccgcagaggc tatccgggat cacatctacg aaaatgatat 145440 ggaggggatg tttgaatgaa gatccttatc gagaaaaacg tgaagaccta tgccaacggg 145500 cgtgtgattg agtattaccg ggtgaagtac aatcgtggct tctggtggcc cttctggcaa 145560 tatgtccagc agcattactc ccacggcgac cccgacatca aggattttga aaccttggag 145620 gaagcaaaaa gggttgcaaa gaaactggca gaggactata ctggccctca gcacataatc 145680 accgaccttg atatcaacgg tgaaccagtt aacaaatact gtgggggatg atatggctgg 145740 cggttaccat gatgcttttg cgataaagca gaaaggaaat gacgggattg tttgtatcga 145800 cgggcacttc cacatttata actccatgca ggctgctgca ctcaacctga cggttttggc 145860 tgctgagtat cctggcaggg agtttacaat cgggcagtta cgtattccac tgccttggac 145920 aaaacagggg ttctaatgta cacaattatt tcttaccgtg aaaacgatgc cgtctaccgt 145980 ggcggctact gtgaaggccg cactgacagt gacatgacgc ttcagtcaac tcaggatcgt 146040 gaacaggcta ttgagttgct ggcccacgcc atctatctgg acactgtggc ccaggatcgt 146100 agcctgaata aggattacga gttcactgct ggggcagaca tcactttggg catcaacggg 146160 tacttcggca catcttatgc ggttgacgaa atctccgacg aggagtatga gaaatactcg 146220 gctgaacgtc agttgatgct ggaagatgca gagatcctcg ccaagcgtaa gtatgatgcc 146280 cgtctggtag actaccgcga ggcacagcgc ctccgggccg agaaggcaga gcagcaggcg 146340 gctgaacgtc gtgaggccga ggaacgtgcc cagttggccc gcctgaaagc aaaatacggg 146400 gagtgagatg aaagcatacg tttaccagtt attccacgta agtatgcgtg gagagatggg 146460 acagaccgat gtggactggg gtttgtttac cagcctgccg ctggttcgtg aagtgatctt 146520 gaagtctaac gacaacccgg aggatttcga ttgctatcgc caccctctga acccgcaggg 146580 gcattacgtg ccgaccccgg tacaaatctt ccgcaaataa ccgccgaacc gccctccggg 146640 cggttttcat gtttatgaaa tgaacttttt tcgacaaata ccccgaaaca tgtttacagc 146700 gtaaacatat gggtgtatat tgaccttacc gactgaagag gagaagacta tggagccgac 146760 tattgaacaa attcgtgaga ctatcagccg cctggaggac cctgttatcc tgtccacgct 146820 accgctgatg cgtaagatgg ccctgtgcct cggcaaggat agctccgctg ccctggtggt 146880 tgtggaactg tgccgtgaag gcgtgaagcg gggtattctg gaatgatggg cttttgtttg 146940 ttcatggcct gcgttgctgg cgcggtattc ctgtacgggg aactgtgcct ggggcacggt 147000 agtcactgag gggaggacaa atgatcaaca accctaacgc tgaatgggag aactgcgatt 147060 atcttttgtg gcctgcatgg tacgcatatg tgtcatgggc catcgtggcc ctgatattcg 147120 ccccttgtgc ctttgcgatc tggatgggcc tatgagcaag aaccgtgacc cgtgggacca 147180 gggcgtccgt ataatccagt acccgaacgg gcaccgtaca gaactgcgcc tgtcctcgac 147240 aggattcgtg catgagttcc cgatagaaac gcctgaagag gctcgtgtcc gggaaatctg 147300 tactctgcaa cgccgaatta aatctgacac cgcccggctg gcggaactgc aaggagattg 147360 gtacgatgaa cattgaagaa atgaacgcac cacatatcat cgaggacatc aacggcaacc 147420 cgttggcctt agtgcataac atcgttatgg cccgctcagt ggcccgctac tgggacagat 147480 tcctgatggt gtccactcat atccgtctgg caaatgagga cgacatagcg gcgtttggac 147540 gccgactggt ggacgcatga ctcccggcat tgtaatcgtc ggcgggttcc tgctgataat 147600 cgccgccggg gtggcggggt attacttcga gcgttacctg gacaggcgct gggctgaaca 147660 agccgcagaa cgcatctggg aaaaccgtga taaatggctg aagagaggtt agtgtgggcg 147720 taccgtatgc aatcgtagac cggaacggca tggtagccgc cattgtgccg gagcgcatcg 147780 tagcggaggt catggcctct gcgctcgttc ccaacatggg ttaccggatg gaatgccgcc 147840 cggcaacgga agaggaaatc taccaactaa tctaccaact ggaacagaac cgccctcccc 147900 tgccggagcg ctccgtgcgc tacaagaccc tgaagggtgt gggccaacaa ttggtggtgg 147960 tagcattacc aagggagcct gtcgagtccc atcagtaccc tgcctgaagt ggcgaacctg 148020 ttgataacta aggagtttct gtggaaaata ccgaagaccg aagcccgtat gccgggtaca 148080 atccgattat cccgaacagt gacgaagagg cagcgctgga gcagcgtgtc tttgacatgc 148140 tgtacaacgg caggaacaac ctgtatagcc gctgccagcg ggagcacgtc gccatcatcc 148200 aagacctgtg gagagagttc gtattccgcg aatcctggta ctcgaaagag gacaagccca 148260 aggactggta catcgggtat gtggacgcac tgaagcactt tgagggtgag tgcccgccgg 148320 gcggctacaa gggcgcgaac ggtgatccca aaactgatta cgacttcggc tggaacgccg 148380 ggtttaaggc atcctcatga ttaaggtccc tccggagatc aaggtctcgg aatggtatcc 148440 gacgtatggc ttcacacaga aagaactgcg gtatctgtat gtggactgcg acgtccgcat 148500 cttcgaggac cagagagacg gcgttatcta ccagatcatg gatagcggtc cagtggataa 148560 acgacgccgg gacctgtctg gtttctggct caaaaagttg gaggactact tatgagtcga 148620 accccatgca ggctcgctac ccgctggaac ctgaaggccc gccccaagaa gcctgtgtgg 148680 aggcaactct acttcccggt gccggagtcc ggcatggctg tatgccttga gcacaatggc 148740 ttcggcttcg tgaagggtaa ggcgtatcgc ttccacacac aggtaagggc tgaccagtac 148800 ccgccatacg atgtggtcac ggtcaccaga gatgactact ctgaggacca ccccatcacc 148860 acgtgggtgt tcctcaacaa tcaggatgac cttttccgta agtcattcca aatcatctga 148920 ccgccacaca ctcttgacct ccttcgggag gtctttttgt ttcccacaga gggtaaccca 148980 gagagcctaa agaggcagac ctgtgcataa ctcatgtgcc acaggctggc gcaccacccc 149040 gtccgggagg ctctctcccg ccgggtagta accgtcccac agagacgtac ccgtctgtat 149100 tcccacattg cccaaatatc aagggagccg gagaggctca cctaccctga ctttttggtg 149160 tttttaccct cccgccaaaa tttttagaac gcgatcaggg gtagcccttc ttgcccaaaa 149220 accagaaaaa tatctcagcg tatggatata gctgcca 149257

Claims (11)

  1. 크로노박터 사카자키(Cronobacter sakazakii)에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 CS3.
  2. 제1항에 있어서, 상기 박테리오파지는 수탁번호 KFCC11736P인, 박테리오파지 CS3.
  3. 제1항의 박테리오파지 CS3를 포함하는, 항생용 조성물.
  4. 제1항의 박테리오파지 CS3를 포함하는, 사료 첨가용 조성물.
  5. 제4항의 사료 첨가용 조성물을 포함하는, 사료.
  6. 제1항의 박테리오파지 CS3를 포함하는, 소독제.
  7. 제1항의 박테리오파지 CS3를 포함하는, 세척제.
  8. 제1항의 박테리오파지 CS3를 포함하는, 식품 조성물.
  9. 제8항에 있어서, 상기 식품은 분유 또는 이유식인 것을 특징으로 하는, 식품 조성물.
  10. 제1항의 박테리오파지 CS3를 식품에 처리하는 단계를 포함하는, 크로노박터 사카자키균이 제거된 식품 제조 방법.
  11. 제1항의 박테리오파지 CS3를 인간을 제외한 개체에 투여하는 단계를 포함하는, 크로노박터 사카자키에 의한 감염성 질병의 예방 또는 치료 방법.
KR1020170181009A 2017-12-27 2017-12-27 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물 KR102066920B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020170181009A KR102066920B1 (ko) 2017-12-27 2017-12-27 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020170181009A KR102066920B1 (ko) 2017-12-27 2017-12-27 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물

Publications (2)

Publication Number Publication Date
KR20190079075A true KR20190079075A (ko) 2019-07-05
KR102066920B1 KR102066920B1 (ko) 2020-01-16

Family

ID=67225595

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020170181009A KR102066920B1 (ko) 2017-12-27 2017-12-27 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물

Country Status (1)

Country Link
KR (1) KR102066920B1 (ko)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210110044A (ko) * 2020-02-28 2021-09-07 연세대학교 원주산학협력단 크로노박터 사카자키 박테리오파지의 대량 생산 방법
CN114540313A (zh) * 2020-11-24 2022-05-27 广东省微生物研究所（广东省微生物分析检测中心） 一种克罗诺杆菌噬菌体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120065718A (ko) * 2010-12-13 2012-06-21 가천대학교 산학협력단 크로노박터 사카자키에 대해 생육 억제능을 나타내는 신규의 박테리오파지
KR20160082141A (ko) * 2014-12-31 2016-07-08 한국외국어대학교 연구산학협력단 크로노박터 사카자키의 박테리오파아지 pbes01 및 그 용도

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120065718A (ko) * 2010-12-13 2012-06-21 가천대학교 산학협력단 크로노박터 사카자키에 대해 생육 억제능을 나타내는 신규의 박테리오파지
KR20160082141A (ko) * 2014-12-31 2016-07-08 한국외국어대학교 연구산학협력단 크로노박터 사카자키의 박테리오파아지 pbes01 및 그 용도

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Cronobacter phage CR3, complete genome. GenBank: JQ691612.1, 2012.05.15. *
J. Virol. 2012, vol. 86, no. 11, pp. 6367-6368.* *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210110044A (ko) * 2020-02-28 2021-09-07 연세대학교 원주산학협력단 크로노박터 사카자키 박테리오파지의 대량 생산 방법
CN114540313A (zh) * 2020-11-24 2022-05-27 广东省微生物研究所（广东省微生物分析检测中心） 一种克罗诺杆菌噬菌体及其应用
CN114540313B (zh) * 2020-11-24 2024-02-23 广东省微生物研究所（广东省微生物分析检测中心） 一种克罗诺杆菌噬菌体及其应用

Also Published As

Publication number Publication date
KR102066920B1 (ko) 2020-01-16

Similar Documents

Publication Publication Date Title
CN112831474B (zh) 一株宽裂解谱沙门氏菌噬菌体rdp-nsa-19050及其应用
CN107257853B (zh) 新型肠出血性大肠杆菌噬菌体Esc-CHP-1及其用于抑制肠出血性大肠杆菌增殖的用途
CN112662635B (zh) 一株耐高温型大肠杆菌噬菌体rdp-ec-20031及其应用
KR101993121B1 (ko) 신규한 클로스트리디움 퍼프린젠스 특이 박테리오파지 cp3 및 이를 포함하는 항균 조성물
KR102193499B1 (ko) 신규한 바실러스 세레우스 균 특이 박테리오파지 bc13 및 이를 포함하는 항균 조성물
KR102066920B1 (ko) 신규한 크로노박터 사카자키 특이 박테리오파지 cs3 및 이를 포함하는 항균 조성물
KR101993122B1 (ko) 신규한 클렙시엘라 속 세균 특이 박테리오파지 kp1 및 이를 포함하는 항균 조성물
CN113166730A (zh) 新型猪链球菌噬菌体str-sup-1及其在抑制猪链球菌细菌增殖方面的用途
KR20180042748A (ko) 신규한 포도상구균 특이 박테리오파지 sa7 및 이를 포함하는 항균 조성물
KR102037394B1 (ko) 신규한 크로노박터 사카자키 특이 박테리오파지 cs4 및 이를 포함하는 항균 조성물
KR20180074608A (ko) 신규한 클로스트리디움 퍼프린젠스 특이 박테리오파지 cp5 및 이를 포함하는 항균 조성물
KR101863593B1 (ko) 신규한 살모넬라균 특이 박테리오파지 sp1 및 이를 포함하는 항균 조성물
KR102016921B1 (ko) 신규한 살모넬라균 특이 박테리오파지 se131 및 이를 포함하는 항균 조성물
KR102003773B1 (ko) 신규한 비브리오균 특이 박테리오파지 vp4 및 이를 포함하는 항균 조성물
KR101871342B1 (ko) 신규한 살모넬라균 특이 박테리오파지 st1 및 이를 포함하는 항균 조성물
KR101896512B1 (ko) 신규한 사이트로박터 프렌디 특이 박테리오파지 cf1 및 이를 포함하는 항균 조성물
KR102037383B1 (ko) 신규한 비브리오균 특이 박테리오파지 vp2 및 이를 포함하는 항균 조성물
KR102237011B1 (ko) 병원성 살모넬라균 특이 신규 박테리오파지 kfs-sm 및 이를 포함하는 항균 조성물
KR102193495B1 (ko) 신규한 아시네토박터 바우마니 균 특이 박테리오파지 ab63 및 이를 포함하는 항균 조성물
KR102016919B1 (ko) 신규한 살모넬라균 특이 박테리오파지 sc1 및 이를 포함하는 항균 조성물
KR20120073724A (ko) 박테리오파지 ｔ10-1, ｍｙ-1 및 이를 포함하는 채소 무름병 방제용 조성물
KR20190069901A (ko) 신규한 비브리오균 특이 박테리오파지 vp2 및 이를 포함하는 항균 조성물
KR20210035537A (ko) 슈도모나스 에루기노사의 박테리오파아지 pbpa162 및 그 용도
KR102193473B1 (ko) 신규한 클렙시엘라 속 세균 특이 박테리오파지 kp12 및 이를 포함하는 항균 조성물
KR102203675B1 (ko) 신규한 여시니아균 특이 박테리오파지 ye12 및 이를 포함하는 항균 조성물

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
AMND Amendment
X701 Decision to grant (after re-examination)