KR20180063445A - Functional cosmetic compositions for preventing skin aging using the natural fermented extracts and the cosmetics using the same - Google Patents

Abstract

The present invention is to provide a skin anti-aging functional cosmetic composition excellent in antioxidative activity which suppresses active oxygen which directly causes various diseases and aging, and a cosmetic having skin anti-aging effect including the same by providing a natural fermented extract comprising a mixture of pine needle leaf extract, spirulina fermented extract, and medicinal plant fermented extract obtained through a fermentation process having an effect of enhancing active ingredients and producing physiologically active substances.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for preventing skin aging using natural fermentation extracts and a cosmetic composition having skin antioxidant effect,

The present invention provides a natural fermented extract prepared by mixing pine needle leaf extract, spirulina fermentation extract and fermented rice extract obtained through a fermentation process having an effect of enhancing an effective ingredient and producing a physiologically active substance, A skin aging-resistant functional cosmetic composition excellent in antioxidant activity that suppresses active oxygen which acts as a direct cause, and cosmetics having skin aging prevention effect including the same.

Among the many hypotheses that explain aging, the free radical theory proposed by Harman has attracted much attention. According to this hypothesis, the aging process proceeds as a result of continuous harmful reactions by free radicals. Oxidative stress theory has been proposed based on the finding that reactive oxygen species (ROS) accumulate oxidative damage of cellular components.

ROS and reactive nitrogen species (RNS) play an important role in elimination of foreign substances in infectious or inflammatory reactions, but when they are overproduced and accumulated, their inflammation, active oxygen and active nitrogen production are continuously worsened due to their destructive tendency, Resulting in loss of homeostasis and aging and cancer, cardiovascular disease, arthritis, and geriatric diseases such as retinal neuropathy.

An antioxidant defense system capable of eliminating strong active oxygen and nitrogen species is believed to play an important role in the prevention of aging and aging-related diseases. Research has been actively conducted on antioxidants related to inhibition of aging and diseases, as it has been found that active oxygen and peroxide act directly on various diseases and aging.

In addition, the life expectancy of human beings has been increasing due to the recent improvement in living standards and the advancement of modern medicine. Therefore, there is an increasing interest in health and a desire for youth and beauty. In addition to the field of cosmetics, such an atmosphere is extended to medical and dietary fields, and a variety of beauty technologies, medicines and foods are being developed.

The market size of the domestic cosmetics field related to the present invention is gradually expanding, and competition in the cosmetics market is intensifying as foreign large-scale cosmetics companies are actively entering the domestic market through FTA negotiations.

The development of natural, organic, and natural cosmetics is being continually developed, especially reflecting the increasing social and mood for youth and beauty.

The present invention is to provide a cosmetic composition having excellent antioxidative activity using natural substances based on the background of the related field and to provide cosmetics containing the cosmetic composition to help maintain the youth and beauty through skin health of consumers.

Korean Registered Patent No. 10-1403523 (Registered Date: 2014.05.28) Korean Patent Laid-Open No. 10-2012-0010687 (Published date: Feb. 26, 2012) Korean Registered Patent No. 10-1430591 (Registration date: Aug. 2014) Korean Registered Patent No. 10-1280482 (Registration date: 2013.06.25) Japan Patent Specification 2011-162457 Japanese Patent No. 01375225 (Apr. 22, 1987) China Patent 200980129606 (2009.05.07)

The present invention relates to a pine needle leaf extract, a spirulina fermentation extract, a fermented extract of a medicinal substance, a pine needle extract obtained by fermenting a pine needle, a spirulina or a medicinal material having excellent antioxidant properties, The present invention also provides a skin aging-resistant functional cosmetic composition comprising the same, and a cosmetic composition having the skin aging-preventing effect.

Another object of the present invention is to provide a natural fermented extract obtained by mixing the pine needle leaf extract, spirulina fermentation extract, and fermented extract of medicinal plant with flaxseed extract, grape seed extract, wanshong extract, onion bark extract, A natural antioxidant, an antioxidant, an antioxidant, an antioxidant, a mushroom extract, a mushroom extract, a parrot extract, and a seaweed extract, and a cosmetic having an anti-aging effect, .

In order to achieve the above object,

According to the present invention, the pine needle collected from the shrimp is washed with water,

The surface of the pine needle was washed with water and then cut,

35 to 64.5wt% of cut pine leaves, 35 to 64.5wt% of water and 0.5 to 1.5wt% of sucrose are mixed to prepare a mixture,

The active ingredient in the mixture is eluted by osmotic pressure and fermented at 18 to 25 ° C for 30 to 90 days so that natural fermentation takes place, and 0.1 to 0.5% by weight of sucrose is further added Lt; / RTI >

The pine needle liquor fermentation product obtained through the fermentation process was concentrated under reduced pressure,

1 to 80 wt% of a pine needle-leaf extract (A) prepared by lyophilizing the fermented product under reduced pressure concentration at -65 ° C to -50 ° C and 8 to 15 mmHg;

Spirulina was centrifuged at 2,000 to 3,000 rpm for 10 to 20 minutes to recover the submerged spirulina,

70% ethanol was added to the recovered spirulina in a ratio of 4.5 to 5.5, and the mixture was extracted with stirring at 38 to 42 캜 for 2 to 4 hours to obtain an extract,

After centrifuging the extract at 2,500 to 3,500 rpm for 10 to 15 minutes, a supernatant was obtained. The supernatant was added to the fermenter and the sugar content of the fermenter was adjusted to 20 to 25 ° Brix. Natural fermentation by microorganism After 10 to 15 days of fermentation,

1 to 80% by weight of the spirulina fermentation extract (B) prepared by powder-phase lyophilization under the conditions of -65 ° C to -50 ° C and 8 to 15 mmHg after concentrating the fermented product under reduced pressure;

90 to 96% by weight of distilled water and 4 to 10% by weight of a medicament are mixed and then bath for 3 to 5 hours to prepare a bath liquid. The bath liquid is prepared by mixing 85 to 90% by weight of the bath liquid, 5 to 10% After natural fermentation for 11 days, the sugar was removed through precipitation filtration using distilled water and ethanol,

The liquid fermentation product of the drug substance from which sugar was removed was concentrated under reduced pressure,

(A + B + C) of 1 ~ 80wt% of the fermented extract (C) prepared by powder lyophilizing the fermented product under reduced pressure at -65 ℃ ~ -50 ℃ and 8 ~ 15mmHg A functional cosmetic composition for preventing skin aging using the natural fermented extract is provided.

The cosmetic composition is prepared by adding the cosmetic composition to the cosmetic composition in an amount of 0.1 to 50 wt% based on the total weight of the cosmetic composition.

The functional anti-aging cosmetic composition using the natural fermented extract according to the present invention enhances useful ingredients contained in pine leaves, spirulina and medicines through fermentation process, and has a high antioxidative function through mixing of these ingredients, And has a free radical scavenging activity which acts as an important cause.

In addition to the antioxidant function, cosmetics having antibacterial, moisturizing and whitening effects by natural substances can be provided.

Hereinafter, a detailed description of the above-described technical configuration will be given.

As described above, in the functional cosmetic composition for preventing skin aging using the natural fermentation extract according to the present invention, the pine needle collected from the shrimp is washed with water,

The surface of the pine needle was washed with water and then cut,

35 to 64.5wt% of cut pine leaves, 35 to 64.5wt% of water and 0.5 to 1.5wt% of sucrose are mixed to prepare a mixture,

The active ingredient in the mixture is eluted by osmotic pressure and fermented at 18 to 25 ° C for 30 to 90 days so that natural fermentation takes place, and 0.1 to 0.5% by weight of sucrose is further added Lt; / RTI >

The pine needle liquor fermentation product obtained through the fermentation process was concentrated under reduced pressure,

1 to 80 wt% of a pine needle-leaf extract (A) prepared by lyophilizing the fermented product under reduced pressure concentration at -65 ° C to -50 ° C and 8 to 15 mmHg;

Spirulina was centrifuged at 2,000 to 3,000 rpm for 10 to 20 minutes to recover the submerged spirulina,

70% ethanol was added to the recovered spirulina in a ratio of 4.5 to 5.5, and the mixture was extracted with stirring at 38 to 42 캜 for 2 to 4 hours to obtain an extract,

After centrifuging the extract at 2,500 to 3,500 rpm for 10 to 15 minutes, a supernatant was obtained. The supernatant was added to the fermenter and the sugar content of the fermenter was adjusted to 20 to 25 ° Brix. Natural fermentation by microorganism After 10 to 15 days of fermentation,

1 to 80% by weight of the spirulina fermentation extract (B) prepared by powder-phase lyophilization under the conditions of -65 ° C to -50 ° C and 8 to 15 mmHg after concentrating the fermented product under reduced pressure;

90 to 96% by weight of distilled water and 4 to 10% by weight of a medicament are mixed and then bath for 3 to 5 hours to prepare a bath liquid. The bath liquid is prepared by mixing 85 to 90% by weight of the bath liquid, 5 to 10% After natural fermentation for 11 days, the sugar was removed through precipitation filtration using distilled water and ethanol,

The liquid fermentation product of the drug substance from which sugar was removed was concentrated under reduced pressure,

(A + B + C) of 1 ~ 80wt% of the fermented extract (C) prepared by powder lyophilizing the fermented product under reduced pressure at -65 ℃ ~ -50 ℃ and 8 ~ 15mmHg .

Fermentation is a chemical reaction that breaks up complex organic compounds into relatively simple substances. Fermentation has the effect of promoting useful components, destroying toxic substances through this process, and producing physiologically active substances.

The present invention provides a cosmetic composition having excellent antioxidative function by mixing the pine needle leaf extract, the spirulina fermentation extract and the fermented extract obtained from the fermentation process.

More specifically, a cosmetic composition is prepared by mixing 1 to 80 wt% of pine needle-leaf extract, 1 to 80 wt of spirulina fermentation extract, and 1 to 80 wt% of medicinal fermentation extract.

When the content of the pine needle leaf extract, the spirulina fermentation extract, and the fermentation extract of the medicinal material is less than 1 wt%, the effect obtained by the fermentation extract of each ingredient is difficult to expect. If the content exceeds 80 wt% The proper amount of the pine needle extract, the ferulic extract, and the fermentation extract of the medicinal herb extract are limited within the range of 1 to 80 wt% with respect to the total weight of the functional cosmetic composition desirable.

The composition of the pine needle leaf fermentation extract, the spirulina fermentation extract and the medicinal fermentation extract constituting the above-mentioned functional cosmetic composition is exemplified by mixing 35wt% of pine needle leaf extract, 50wt of spirulina fermentation extract and 15wt% of medicinal fermentation extract.

The 'brush' has long been used for edible, medicinal and industrial purposes. Pine needles contain vitamin C and K, carotene, flavonoid, anthocyan, tannin, essential oil, and various kinds of terpene compounds. And it is known that there is serum cholesterol lowering, antidiabetic, lipid lowering, antioxidant, antibacterial effect in flavonoid, alkaloid, lignan and organic acids.

Pine needles have long been known to prevent paralysis and prevent geriatric diseases such as arteriosclerosis, hypertension and diabetes. On the other hand, it has been known to be involved in promoting, anti-inflammation and hemostatic action of the redox process which is tasted and warm in the body tissues. Also, it is effective as liver oil, gastrointestinal tract, skin diseases according to Korean medicine and folk remedy.

Specific production examples of the pine needle leaf extract are as follows.

The pine leaves collected from Pinusdensiflora sieb, et Zucc. Were washed with water, and then the surface was drained and cut.

Specifically, for the fermentation of dried pine leaves, pine needles, water, and sucrose were mixed at a ratio of 1: 1: 0.125,

Add the same amount of sucrose three times at 15-day intervals while fermenting at 20 ° C for 60 days so that the oil component in the raw material is eluted by osmotic pressure and natural fermentation takes place simultaneously.

Then, the fermented pine needle liquor fermented product was concentrated under reduced pressure using a rotary evaporator (Model-NE, Tokyo RikakikaiCo, Japan) and freeze-dried at -60 ° C and 10 mmHg in a freeze dryer (Bondiro, Ilsin Engen Co., .

Next, the spirulina fermentation extract will be described.

Spirulina (Spirulinaplatensis) is one of the oldest algae on Earth and is the first photosynthetic creature born about 3.5 billion years ago. It is a spiral type derived from Latin and is used as a functional food like chlorella, a green alga that lives in fresh water. Blue-green algae Blue-green algae are blue microorganisms that are procaryotic cells belonging to the genus Neruschmae, Euremo and Spirulina. They are thin and long cells of 5 ~ 8 ㎛ in width and 0.3 ~ 0.5 mm in length Is a living organism.

Ingredients include 60-70% protein, 6-9% lipid, carbohydrate 15-20% carbohydrate and large amounts of vitamins, minerals and fiber. Among the lipid components, free-fatty acid accounts for 70 ~ 80%, which is composed of linoleic acid and γ-linolenic acid.

Carbohydrates include glucose, rhamnose, mannose, and xylose. Coloring agents include light yellow carotenoids, green chlorophyll, and blue phycocyanin. It also contains a large amount of vitamin B12 and phenolic acid, tocopherols, and β-carotene, which act as antioxidants.

Specific examples of the above-mentioned ferulic extract of Spirulina are as follows.

The spirulina was centrifuged at 3,000 rpm for 15 minutes to recover the submerged spirulina,

The spirulina thus recovered was added with 70% ethanol 5 times and extracted with stirring at 40 캜 for 3 hours to obtain an extract,

After centrifuging the extract at 3,000 rpm for 15 minutes, a supernatant was obtained. The supernatant was added to the fermenter, the sugar content of the fermenter was adjusted to 25 Brix, and the fermentation was performed for 10 days by natural microorganism To obtain a fermented product,

The fermented product was concentrated under reduced pressure and lyophilized at -60 ° C and 10 mmHg to prepare a powder.

Next, the medicinal fermentation extract will be described.

The medicinal fermented extract may be selected from the group consisting of Terminalia chebula, Alpinia officinarum, Sophora japonica, Salviae Miltiorrhizae, Sorbus commixta, Portulaca oleracea, Paeonia lactiflora, (Zanthoxylum schinifolium), Caesalpinia sappan, Epimedium koreanum, Syzygium aromaticum, Lycium chinense, Sanguisorba officinalis, Alpinia katsumadai, Selected from Taraxacum platycarpum, Carthamus tinctorius, Puerariae thunbergiana, Cimicifuga heracleifolia, Scutellaria baicalensis George, Golden thread, Glycyrrhiza uralensis Fisch, and Cinnamonum cassia Presl One or two or more kinds of medicaments are used.

Terminalia chebula is a mature fruit of Gaza (Terminalia chebula Retz), a deciduous tree with Combretaceae. The mature fruit of Gaza contains chebulinic acid, chebulagic acid, 1,3,6-β-trigalloyl-glucose and 1,2,3,4,6-pentagalloyl-β-glucose, corlagin, terchebin, glucogalin, ellagic acid, , gallic acid, terflavin A, B, C, D, and isoquercitrin.

The above-mentioned Alpinia officinarum Rhizoma (AO) is a rhizome of Alpinia officinarum Hance, a perennial herb belonging to Zingiberaceae.

5-Hydroxy-7- (4'-hydroxy-3'-methoxyphenyl) -1-phenyl-3-heptanone and 3-Methylethergalangin have been reported as inhibitors of pancreaticlipase. The phenylpropanoids are antioxidants, diarylheptanoids, pinocembrin, galangrin The substances in the family are anti-inflammatory and anticancer substances, galangrin is whitening, quercetin and kaempferol are known to have fattyacidsynthase inhibitory activity.

The Sophora japonica refers to the barren flower peaks of a deciduous tree that belongs to Leguminosae. The pharmacological effects of the Rutin and Quercetin component of the capillaries vascular permeability and anti-inflammatory and anti-ulcer action. In addition, hemostasis, capillary permeability reduction, hypotension, cholesterol lowering, and anti-cancer action.

Salviae Miltiorrhizae is a perennial herb that belongs to the family Lamiaceae (Labiatae) and is a Chinese origin and rarely medicinally cultivated. Among the antioxidative components of Danshang, the quinone-type compounds tanshinone Ⅰ, dihydrotanshinone Ⅰ, tanshinone ⅡA, tanshinone ⅡB, methyenetanshinquinone, and danshenxinkun B were identified as antioxidants. Among them, all quinone types except tanshinone ⅡA It has an effect comparable to that of BHA and BHT.

The Sorbus commixta is a deciduous broad-leaved arboreous tree. Ingredients include sorbitol, cyanogenic compounds, p-o-coumaric acid in leaves, and β-sitosteril, aucuparin, and methoxyaucuparin in the throat.

The marjoram (Portulaca oleracea) is the outpost of Portulaca oleracea L., which belongs to Portulacaceae. It contains a large amount of noradrenaline and a large amount of potassium salt. It has alanine and aspartic acid as amino acids. Glucose, fructose and sucrose are the polysaccharides, and malic acid, oxalic acid, citric acid, glutamic acid, asparic acid, and alanine have been reported. Sterols such as stigmasterol, campesterol, vitamin B1 and C, saponin, tannin, and flavonoid have been reported.

Paeonia lactiflora belongs to the family Ranunculaceae, and several stems are straight from the root of the root, with no hairs on leaves and stems, and thick roots.

Major efficacy and efficacy include pain, fever, diarrhea, diuretic, depression, abdominal pain, stomach pain, arthritis, anemia, bruises. In recent years, research on physiological activity against peanuts has revealed anti-inflammatory, anti-allergic analgesic, antimicrobial activity and whitening effect, and Paeoniflorin, Paeonol, Albiflorin and so on.

The bananas (Areca catechu) are ripe seeds of the bean (Areca catechu Linne (Palmae)) and are harvested and boiled in water to peel the fruit.

According to Donguibogam, the wind of the wind in the body is removed and all the gear is dropped. Make sure that the bones and the nine holes in your body work well and that the food you eat is well digested. The wall goes to the side of the flank, the sore body, swelling of the body, the bad energy in the body is healed and has the effect of turning the auspicious muscles are blocked.

The above-mentioned Zanthoxylum schinifolium is a fruit of Sancho (Japanese apricot) tree. According to the old document, the fruit husk has been used separately as a flower, a green, and a seed as a plant. The fruit skin contains 2-4% essential oil and the main ingredients are dipentene (dl-limonene), citronellal, l-β-phellandrene, geraniol and citronellol. Fruit contains xanthoxine, a phlegmatic toxin, xanthoxine, paroxetine, sterol campesterol, stigmasterol, and β-sitosterol.

Caesalpinia sappan is a deciduous arboreous tree or shrub belonging to Leguminosae and distributed in tropical Asia such as India, Malaysia Peninsula and southern China. The efficacy includes anti-inflammatory, antioxidant, and antiviral.

Epimedium koreanum is a perennial grass belonging to the genus Melvinaceae. It contains a large amount of flavonoid compounds in leaves and stems, mainly icariin, des-o-methylicariine, and β-anhydroicaritine. There are icariin, icarisid Ⅰ in E. brevicornum and icariin, epimedin A, B, C, quercetin, epimedokoreanoside Ⅰ, Ⅱ, campesterol, β-sitosterol and ikarisosideA in E. koreanum.

Syzygium aromaticum is an evergreen tree that grows in tropical areas and has a short stick shape with a total length of 1 to 2 cm. The buds of cloves contain 16 ~ 19% of essential oils and 80 ~ 87% of eugenol, 9 ~ 12% of caryophyllene, methyl salicylate, alpha-caryophyllene, benzylalcohol, methyl acetate, benzylaldehyde, methoxy benzylaldehyde .

Lycium chinense refers to the root bark of the ginger tree. It has a unique flavor. Its main ingredients are betaine, β-sitosterol glucoside, linoleic acid, scopoletin, and kukoamine. It is one of the medicinal herbs which has been used as medicinal herb because of its inhibitory activity of angiotensin I converting enzyme (ACE) in methanol extract and its blood pressure lowering effect and diverse effects such as diabetes and fever. In addition, in alloxan - induced diabetic mice, hypoglycemic effect of hypoglycemia, serum cholesterol and triglyceride lowering effect were confirmed, and skin damage by UV and liver damage by CCl ₄ were suppressed, and antioxidant ability was suggested.

The Sanguisorba officinalis is the root of the Sanguisorba officinalis Linne and its relative plants (Rosaceae Rosaceae). The root contains tannin, triterpenoid ziyu-glycoside I, Ⅱ, sanguisorbin A, B, E and sanguinin H-1, H-2 and H-3. The efficacy includes blood hemostasis, detoxification, dental bleeding, hemorrhage, hemorrhoids, bursitis, hydration, and sore throat.

The alpinia katsumadai is a perennial herb that belongs to the ginger family, and is called primordial, duo, and anyone. Dried seeds are dried and used as herbal medicines.

Taraxacum platycarpum is the outpost of Taraxacum platycarpum, white dandelion and Western dandelion (Compositae), and lives in Korea, China and Japan. Its pharmacological actions include antibacterial, antifungal, immunosuppressive, , Fever, sweating, inflammation, dryness, diuretic, glaze, gastritis, edam, jaundang. The main ingredients include austricin, luteolin, chrysoolol, quercetin, caffeic acid, scopoletin, esculetin, cichoriin.

The safflower (Carthamus tinctorius) removes most of the yellow pigment from the flower of Carthamustinctorius L., belonging to the Compositae, and compresses it into a plate form.

It is said that safflower improves blood circulation and relieves pain in the primary gland. It is widely used for blood circulation disorder, circulatory fluid, blindness, menopausal disorder, especially postpartum woman's disease It is known to have a laxative action, a perspiration action, a lower heat action, and an effect of soothing the hysterical mood

Puerariae thunbergiana has been widely used for edible and medicinal purposes together with flowers (갈 花 花 花.....................) In folk remedies PuerariaeRadix has been used for the treatment of colds, headache, seizures, diabetes, diarrhea, dysentery, etc. In one room, it has been used for hypertension, coronary atherosclerosis, angina pectoris, senile diabetes, hangover removal, sweating and fever.

In addition, recent studies have reported the effects of hypotensive, anti-inflammatory, antioxidant, alcohol detoxification and hepatic protective actions.

Representative physiologically active substances of Pueraria are isoflavone, saponin, plant sterol and phenol compounds. Isoflavone is a representative component of daidzin, genistin and puerarin.

The horse (Cimicifuga heracleifolia) is a rootstock of Cimicifugaeracleifoliakomarov belonging to the Ranunculaceae family. It is taken in the summer and autumn, and the beard roots are removed and dried in the sun, dried and grown in Korea. A variety of 9,19-cyclolanostane glycosides have been found in horse riding, while acetylshengmanolxyloside and cimicifugoside H-1 are also found in horse riding. Other compounds such as cimicifugin, khellol, amminol, caffeic acid and ferulic acid are known.

Appearance is dark brown, many roots remain, there is no incense, strong detoxification action, and it is used for headache, skin diseases and so on. There are also a number of medicines that can be applied to cosmetics, such as whitening, spotting, spots, and freckles.

Scutellaria baicalensis George is a perennial plant belonging to the family Ranunculaceae. It has a strong antimicrobial activity against various pathogens and has excellent anti-inflammatory, antioxidant, hemostatic, blood pressure lowering and anticancer effects. In particular, it is known that the anti-inflammatory effect is excellent. Many studies have reported that the extract of Rhodiola extract inhibits the production of inflammatory mediators IL-1, IL-6, TNF-α, PGE2 and NO.

The golden (Scutellaria baicalensis) is a perennial herb of Lamiaceae which has been used medicinally in Korea and China for a long time. Gold is known as a crop that exerts powerful pharmacological effects and has been used as an alternative medicine for antimicrobial, antiviral, anticancer and antioxidant effects. Baicalin, baicalein, and wogonin are known to be effective ingredients of gold, and about 50 kinds of flavones have been identified to date.

Since gold has efficacy as a fever, diuretic, branching, insect and anti-inflammatory agent, it has not only been used as a private prescription drug since ancient times, but also has been used for drying dysentery, pyrexia, jaundice and carbuncle, Has been used as a therapeutic agent.

The roots of gold have physiologically active components such as baicalein, baicalin, wogonin and acteoside, and physiologically active components such as wogonoside, neobaicalein, oroxylin, oroxylin A, skullcapflavone, dihydrooroxylin A, chrysin, stigmasterol and beta-sitosterol. And antioxidant activity, as well as in vitro and in vivo antimicrobial, antiallergic, antiinflammatory, diuretic, excretory juice, antiepileptic, hypotensive and antipyretic effects as well as various pharmacological effects such as central nervous system and lipid metabolism.

The licorice (Glycyrrhiza uralensis Fisch) is a perennial herb belonging to leguminosae, which is a dried herb medicine of roots and rootstocks of Glycyrrhiza uralensis, G. inflata, and G. glabra, containing glycyrrhizin in an amount of 6 to 14% It has a warm aura and has various pharmacological actions such as anti-inflammatory action, antioxidant action, anti-cancer effect, hepatitis treatment effect, and tuberculosis treatment effect.

Among the components of licorice, there are glycyrrhizin, licorion, teprenon, and flavonoid, terpenoid, and quinone. Especially, the antimicrobial activity of 75% ethanol extract of licorice is liquiritigenin, a flavanone compound, and glabridin, an isoflavone compound, has antimicrobial activity and antioxidant activity.

Cinnamonum cassia Presl is an evergreen tree of Lauracease, and Cinnamonum cassia Presl is used as medicine. The cinnamon is the barks of cassia, and the bamboo is the thinnest among the twigs of the cassia, and is thin and thin.

The above-mentioned medicinal materials may be used alone or in combination of two or more thereof (Compound 1)

Preferred examples of formulations (compounding 2) include 0.1 to 30 wt% of terminalia chebula, 0.1 to 30 wt% of Alpinia officinarum, 0.1 to 30 wt% of Sophora japonica, 0.1 to 30 wt% of salviae miltiorrhizae, 0.1 to 30 wt% of Sorbus commixta, 0.1 to 30 wt% of Portulaca oleracea, 0.1 to 30 wt% of Paeonia lactiflora, 0.1 to 30 wt% of Areca catechu, 0.1 to 30 wt% of Zanthoxylum schinifolium, 0.1 to 30 wt% of Caesalpinia sappan, 0.1 to 30 wt% of Epimedium koreanum, 0.1 to 30 wt% of Syzygium aromaticum, 0.1 to 30 wt% of Lycium chinense, 0.1 to 30 wt% of Sanguisorba officinalis, 0.1 to 30 wt% of Alpinia katsumadai, 0.1 to 30 wt% of Taraxacum platycarpum, 0.1 to 30 wt% of Carthamus tinctorius, 0.1 to 30 wt% of Puerariae thunbergiana, 0.1 to 30 wt% of Cimicifuga heracleifolia 0.1 to 30 wt% of Scutellaria baicalensis George, 0.1 to 30 wt% of Golden thread, 0.1 to 30 wt% of licorice (Glycyrrhiza uralensis Fisch) and 0.1 to 30 wt% of Cinnamonum cassia Presl It shall be used.

More specifically, it is preferable to use a mixture containing 4.5 wt% of Terminalia chebula, 4.5 wt% of Alpinia officinarum, 4.5 wt% of Sophora japonica, 4.5 wt% of Salviae Miltiorrhizae, 4.5 wt% of Sorbus commixta, , 4.5 wt% of Portulaca oleracea, 4.5 wt% of Paeonia lactiflora, 4.5 wt% of Areca catechu, 4.5 wt% of Zanthoxylum schinifolium, 4.5 wt% of Caesalpinia sappan, 4.5% by weight of Syzygium aromaticum, 4.5% by weight of Lycium chinense, 4.5% by weight of Sanguisorba officinalis, 4.5% by weight of Alpinia katsumadai, 4.5% by weight of Taraxacum platycarpum, , 4.5 wt% of Carthamus tinctorius, 3.0 wt% of Puerariae thunbergiana, 4.5 wt% of Cimicifuga heracleifolia, 3.5 wt% of Scutellaria baicalensis George, 3.5 wt% of Golden thread, 4.5 wt% of Glycyrrhiza uralensis Fisch, and 4.5 wt% of Cinnamonum cassia Presl (Compound 3)

As the more preferable formulation examples (formulations 4 and 5)

The medicinal material is a mixture of 10 to 30 wt% of Lycium chinense, 10 to 30 wt% of Areca catechu, 10 to 30 wt% of Puerariae thunbergiana, and 10 to 30 wt% of Cimicifuga heracleifolia, Lt; / RTI >

Or a mixture of 10-30 wt% of Lycium chinense, 10-30 wt% of Areca catechu, 10-30 wt% of Scutellaria baicalensis George and 10-30 wt% of Golden thread, use.

More specifically, a mixture of 25 wt% of Lycium chinense, 25 wt% of Areca catechu, 25 wt% of Puerariae thunbergiana, and 25 wt% of Cimicifuga heracleifolia is used as a medicinal material, 6)

Or mixture of 25 wt% of Lycium chinense, 25 wt% of Areca catechu, 25 wt% of Scutellaria baicalensis George and 25 wt% of Golden thread. (Formulation 7)

Specific examples of the preparation of the above fermented pharmaceutical extracts are as follows.

95% by weight of distilled water and 5% by weight of medicinal materials are mixed, and the mixture is transferred for 4 hours to prepare a bath liquid. At this time, the medicinal material is selected by using one of the specific formulation examples (Formulations 3, 6 and 7), and 5 wt% of the composition of 100 wt% is selected according to the formulation examples (Formulations 3, 6 and 7) do. The medicaments are prepared by removing impurities, drying them, pulverizing them with a mixer (HMF-985), homogenizing the powder with a mesh sieve (0.42 mm), and storing them in a refrigerator at 4 ° C.

The mixture of 90 wt% of the prepared bath liquid and 4 wt% of the same medicinal materials as the medicinal materials used for the above-mentioned bath liquid preparation and 6 wt% of sugar were mixed and fermented for 11 days. Then, the mixture was subjected to precipitation filtration using distilled water and ethanol to remove the sugar.

As a specific example, a mixture of distilled water and ethanol was subjected to precipitation filtration three times. Distilled water and ethanol were mixed in a ratio of 1: 3 by weight at one time, and distilled water and ethanol were mixed at a ratio of 1: 5.5 by weight . In the third cycle, distilled water and ethanol are mixed in a weight ratio of 1:19.

After removing the sugar via the above process, the fermented liquid of the drug substance liquid phase was concentrated under reduced pressure (740 mmHg, 60 ° C., 6 hours), and the fermented substance subjected to the concentration under reduced pressure was lyophilized at -60 ° C. and 11 mmHg to prepare a powdery form .

Specific examples of the cosmetic composition prepared using the pine needle leaf extract, the spirulina fermentation extract, and the fermented extract of the medicinal plant obtained through the above extraction method are shown in Table 1 below.

A cosmetic composition (Examples 1 to 3) comprising a pine needle leaf extract, a pine needle leaf extract, a Spirulina fermentation extract,

ingredient Example 1 Example 2 Example 3 Pine needle extract 40wt% 35wt% 30wt% Spirulina fermented extract 40wt% 35wt% 30wt% Medicinal fermentation extract * 20wt% 30wt% 40wt% Sum 100wt% 100wt% 100wt%

(* The above fermented extract is a fermented extract prepared from a medicinal substance prepared according to compound 3.)

The cosmetic composition further comprises 0.5 to 10 wt% of an antioxidant activity extract obtained from any one or more selected from flaxseed, grape seed, persimmon, and onion skin to further enhance antioxidant functionality.

In addition to the above-described composition, the present invention further comprises 0.1 to 0.5 wt% of a flaxseed extract, 0.1 to 0.5 wt% of a grape seed extract, 0.1 to 0.5 wt% of a wormy extract, and 0.1 to 0.5 wt% of an onion skin extract Including, but not limited to,

0.1 to 0.5 wt% of Sparassis crispa extract, 0.1 to 0.5 wt% of Phellinus linteus extract and 0.1 to 0.5 wt% of Inonotus obliquus extract,

0.1 to 0.5 wt% of Caposiphon fulvescens extract, 0.1 to 0.5 wt% of Enteromorpha intestinalis extract and 0.1 to 0.5 wt% of Undaria pinnatifida extract.

The scientific name of the Flaxeed is Linum usitatissimum, a seed oil of linaceae plant, which is self-supporting and contains about 40% of oil.

It is known that flaxseed is rich in many essential ingredients such as fat, protein, fiber, minerals and vitamins. It contains a high content of a high content of a-linolenic acid and various essential components and amino acids such as omega-3 fatty acid, lignan, tocoleferol, folic acid and minerals and has been used as a medicinal substance according to Dong- Has been recognized as a pharmacological effect since ancient times in the West. a-linolenic acid, which releases blood cholesterol to treat arteriosclerosis, liver detoxification, skin moisturizing, and inhibits aging.

The general components of flaxseed are crude fat 36.6 ~ 42.5%, carbohydrate 30.0 ~ 34.5%, crude protein 18.0 ~ 22.7%, crude oil 3.1 ~ 40% and moisture content 0.2 ~ 6.8%.

The flaxseed is very rich in lignans. Lignans are similar to estrogen, a hormone secreted by our body. It has a lot of essential fatty acids and it has a good moisturizing effect. It can solve dryness, collagen reduction and elasticity degradation due to aging of menopausal women. It has a high level of unsaturated fatty acid metabolism, a decrease in the synthesis of fatty acids in the liver, a decrease in cholesterol concentration and inhibition of cholesterol absorption and synthesis, and an improvement in liver function and an antihypertensive effect while promoting alcohol decomposition.

The flaxseed extract was prepared by adding 4000 ml of methanol (MeOH) to the sample, and extracting it three times for 5 hours each with a shaker. The extract was filtered with a filter paper (Watman No. 2) and concentrated under reduced pressure (740 mmHg, 60 ° C, ≪ / RTI >

The grape (vitis vinifera) is a deciduous vine plant belonging to the Rhamnales vitaceae. The grape has 11 genera and 700 kinds of grapes.

Vitis vinifera Linne is rich in resveratrol, which is known as an antioxidant and phenolic compounds such as anthocyanins, benzoic acid, flavonol, procyanidin, and prophylgallate, as well as organic acids, vitamins and minerals to lower blood cholesterol levels, , Stroke, prevention and treatment of heart disease, anticancer, antioxidant, antifungal action.

Grape seed contains antioxidants such as catechin, resveratrol and tannin, and has physiological effects such as antioxidation, anti-inflammation, and blood cholesterol lowering.

The extract of the grape seeds was extracted with 3 times of shaker for 6 hours by adding 10 times MeOH to 300 g of the grape seed powder. The extract was filtered with a filter paper (Watman No. 2) and concentrated under reduced pressure (740 mmHg, 60 ° C, 6 hours) MeOH < / RTI >

The above-mentioned worm (Orostachys japonicus A. Berger) is a perennial herbaceous plant of Crassulaceae and belongs to the so-called rocksol, new tower,

Moisture (48.5%) is the most common component of warsh, and other carbohydrates (38.2%), crude fiber (15.8%), crude protein (13.2%) and reducing sugar (12.%) are included. In addition, it is also possible to use aluminum (Al), calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na) Zn), and the content of calcium and potassium is high. It is composed of 17 kinds of amino acids and has the highest content of glutamic acid. In addition, aspartic acid, leucine, alanine, valine, tyrosine, . Wassong's free sugar is made up of glucose, sucrose and fructose, galactose and maltose.

In addition to the basic nutritional ingredients such as minerals and other antioxidants and antioxidants, which are taken during the summer and autumn, are used for medicinal purposes. In one room, it is used to treat cyanosis, detoxification, humidification, hemostasis, and extinction, and has long been used as a cure for cancer as a folk remedy.

The wort extract is extracted three times by adding 20 times methanol to the weight of the sample and refluxing for 5 hours at 65 to 80 ° C. The extract was filtered through a filter paper (Watman No. 2) and concentrated under reduced pressure (740 mmHg, 60 ° C, 6 hours) to obtain a MeOH extract.

Onion (Allium cepa L.) is a perennial plant belonging to the lily family. It has been cultivated for a long time. It is one of the representative spices of Korea. It is one of the main seasonal vegetables in our diet along with pepper and garlic.

Onion water has about 88 ~ 90% of carbohydrates and 6.8 ~ 10.0% of carbohydrates. Fructose is the most abundant in the composition, glucose and sucrose are almost the same amount, and polysaccharides are many. The sugars that make up these polysaccharides are galactose, arabinose, ribose and rhamnose, and contain little starch.

Onion's main physiologically active substances are flavonoids and sulphur compounds, which have powerful antioxidant properties and functions to reduce body fat levels.

The onion extracts were extracted with the same amount of onion skin and onion meat. 10% of 70% ethanol was added to the extract and the mixture was leached at room temperature for 12 to 15 hours. After filtration, the remaining extracts were repeatedly extracted twice, filtered and concentrated under reduced pressure (740 mmHg, 60 DEG C, 6 hours) to obtain a concentrate. The concentrate is lyophilized and stored frozen at -18 ° C and used as a sample.

The Sparassis crispa belongs to Aphyllophorales, Sparassidaceae and Sparassis, and is a mushroom not only for edible but also medicinal purposes because of its soft texture and chewy texture.

It has been reported that beta-glucan is a high-molecular polysaccharide containing dietary fiber and beta-1, 6-glucan in its main chain linked with beta-1, 3-glucan. Total insoluble dietary fiber (TDF) was 61.8 g / 100 g and water soluble dietary fiber was 2.6 g / 100 g in the general composition of P. falciparum, and 96% of total dietary fiber was insoluble dietary fiber.

The word "Phellinus linteus" refers to the word "mulberry," derived from China, and it is called mulberry. Basidiomycetes It is a white-footed mushroom belonging to the mushroom (phellinus) and belongs to the pine scaly mushroom (Hymenochaetaceae). It is a perennial mushroom of extremely rare bivalve mushroom which does not grow very well and grows in hardwoods or mountain mulberry trees that live in high mountains.

The chaga mushroom is a species of black birch carcinoid that is native to Russia and its scientific name is Inonotus obliquus. Phenol, aldehyde, β-glucan, oxyphenolcarboxylic acid, and polyphenol are the active ingredients of chaga mushrooms which are known to have an effect on the anticancer physiological activity, and lignin having pharmacological activity is one of the main components of chaga mushroom . Chaga mushrooms also contain steroid compounds that enhance the body's adaptability and immunity by affecting the hormones and hormones. They stimulate the central nervous system activity, hormonal activity and metabolism to enhance the external and internal resistance of the body. It is also well known that it has efficacy in activity.

The seaweed fulvescens (Capsosiphon fulvescens) is one of the algae that have recently been spotlighted as a green algae belonging to the reptilian family. The essential amino acid content is much higher than other seaweeds and it is a nutritionally superior plant because it contains a large amount of vitamin A, C and fatty acids.

Studies on moxifloin have immunosuppressive and anti-cancer effects, inhibition of lipid peroxidation, and inhibition of triglyceride and cholesterol.

Enteromorpha intestinalis contains not only a large amount of carbohydrate and protein among the three major nutrients, but also vitamin A, vitamin B1, ribofuran and niacin, which are excellent in not only body composition but also calorie content. Mineral contains a lot of calcium and iron, and contains plenty of vegetable fiber to help the peristaltic movement of the colon is effective to smooth the bowel movements.

Undaria pinnatifida is a marine algae (Aliasaceae) plant. It is used as a food or medicinal material after it is harvested by taking the ground part when the leaves are silent from winter to April to April.

Seaweeds contain 35.3% of carbohydrates, 30.8% of ash, 15% of protein, 13% of moisture, 3.2% of fat, 2.7% of fiber, carotene and vitamins A, B1, B2, B3, B12, C, etc., and 1.8% of iodine, bromine and calcium, which have a pharmacological action, and 1.6% of organic acid, alginic acid, alanine, glycine, proline, palmitic acid, fucosterol, fucoidan and mannitol.

Seaweed lowers blood pressure and blood cholesterol level, corrects the lack of thyroid function, enters the tissues and blood, accelerates the absorption of pathological products, disrupts and dissolves the pathological tissues, and acts as a diet for stomach cancer. It has been reported that seaweed has anticancer effect like the permission bar.

Wherein the composition comprises 0.1 to 0.5 wt% of flaxseed extract, 0.1 to 0.5 wt% of grape seed extract, 0.1 to 0.5 wt% of salted extract, 0.1 to 0.5 wt% of onion skin extract, (Examples 4 to 6) of the cosmetic composition to be added are further shown in Table 2 below.

ingredient Example 4 Example 5 Example 6 Pine needle extract 35wt% 35wt% 35wt% Spirulina fermented extract 35wt% 35wt% 35wt% Fermented herbal extract 29.6 wt% 28.8 wt% 28wt% Flaxseed extract 0.1 wt% 0.3 wt% 0.5 wt% Grape seed extract 0.1 wt% 0.3 wt% 0.5 wt% Wassong extract 0.1 wt% 0.3 wt% 0.5 wt% Onion skin extract 0.1 wt% 0.3 wt% 0.5 wt% Sum 100wt% 100wt% 100wt%

(Phellinus linteus) extract, 0.1 to 0.5 wt% of Sparassis crispa extract, 0.1 to 0.5 wt% of Phellinus linteus extract, and Inonotus obliquus extract are added to a composition composed of a pine needle leaf extract, a pine needle leaf extract, a Spirulina fermentation extract, Specific examples (Examples 7 to 9) of the cosmetic composition prepared by further adding 0.1 to 0.5 wt% of the extract are shown in Table 3 below.

ingredient Example 7 Example 8 Example 9 Pine needle extract 35wt% 35wt% 35wt% Spirulina fermented extract 35wt% 35wt% 35wt% Fermented herbal extract 29.7 wt% 29.1 wt% 28.5 wt% Mushroom extract 0.1 wt% 0.3 wt% 0.5 wt% Mushroom extract 0.1 wt% 0.3 wt% 0.5 wt% Mushroom extract 0.1 wt% 0.3 wt% 0.5 wt% Sum 100wt% 100wt% 100wt%

A composition comprising 0.1 to 0.5 wt% of Caposiphon fulvescens extract, 0.1 to 0.5 wt% of Enteromorpha intestinalis extract, 0.1 to 0.5 wt% of Undaria pinnatifida extract, (Examples 10 to 12) of the cosmetic composition prepared by further adding 0.5wt% of the composition are shown in Table 4 below.

ingredient Example 10 Example 11 Example 12 Pine needle extract 35wt% 35wt% 35wt% Spirulina fermented extract 35wt% 35wt% 35wt% Fermented herbal extract 29.7 wt% 29.1 wt% 28.5 wt% Mash extract 0.1 wt% 0.3 wt% 0.5 wt% Parasitic extract 0.1 wt% 0.3 wt% 0.5 wt% Seaweed extract 0.1 wt% 0.3 wt% 0.5 wt% Sum 100wt% 100wt% 100wt%

[ Test Example 1 ]

Whether it is applicable to cosmetics by measuring the antioxidative ability of the cosmetic composition shown in Examples 1 to 12 will be described.

1) Free Radical Scavenging Activity by DPPH Method

For the cosmetic composition according to the present invention (Examples 1 to 12), free radical scavenging activity, which is considered to be a causative agent of skin aging, was measured (using the DPPH method).

2 ml of a 0.3 mM DPPH solution dissolved in methanol and 2 ml of ethanol are mixed, and 2 ml of each of the cosmetic compositions according to Examples 4 to 12 is added thereto.

After standing at room temperature for 15 minutes, the absorbance was measured at 517 nm using a spectrophotometer.

The size of the activity was represented by the activity inhibition rate of DPPH as a control group in which the cosmetic composition according to the present invention was added to the experimental group and the cosmetic composition was not added.

Free radical scavenging activity was expressed as the concentration of free radical scavenging activity (FSC ₅₀ , ㎍ / mL) required to reduce the concentration of DPPH by 50%. The results are shown in Table 5 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Control group free radical
elimination
activation

5.82

5.90

5.98

4.62

4.85

4.95

4.53

4.65

4.86

5.01

5.23

5.62

22.02

The antioxidant capacity of the cosmetic composition according to the present invention can be determined by reacting with DPPH which is a stable free radical. Table 5 shows that the radical scavenging activity of the cosmetic composition according to the present invention is significantly larger than that of the control group.

2) Fe using Luminol emission method ²⁺  - EDTA / H ₂ O ₂ Active oxygen scavenging activity in the system

The Fe ³⁺ -EDTA / H ₂ O ₂ system generates various ROS, and iron catalyzes this reaction. Therefore, the total antioxidant capacity of ROS can be measured using this system. Detection of the resulting ROS can be determined by measuring chemiluminescence through the reaction between luminol and ROS.

2 ml of distilled water was added to the tube for chemiluminescence measurement, and the cosmetic composition according to Examples 1 to 12 of the present invention was added. Here 3mM EDTA and 5mM FeCl ₃ · 40㎕ After adding 10 μl of 6H ₂ O, add 80 μl of 36 mM luminol and shake.

The tube for chemiluminescence measurement is placed in a cell holder of a chemiluminescence device, and the temperature is maintained for 6 minutes. Then, 40 μl of 150 mM H ₂ O ₂ is added and the chemiluminescence is measured for 30 minutes.

The control group (control) in Example 1. Distilled water was added in place of the cosmetic composition according to to 12, the blank test (blank) is the experimental conditions and the same one H ₂ O ₂ and FeCl ₃ · 6H ₂ O was not added.

The size of the active oxygen scavenging activity was expressed as the concentration of the sample required to reduce the intensity of chemiluminescence by 50% (reactive oxygen species scavenging activity, OSC ₅₀ , / / mL). The results are shown in Table 6 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Control group activation
Oxygen
elimination
activation

1.78

1.72

1.70

1.50

1.48

1.42

1.49

1.47

1.45

1.52

1.51

1.47

2.45

As shown in Table 6, it can be seen that the active oxygen scavenging activity (total antioxidant ability, OSC ₅₀ ) of the cosmetic composition is larger than that of the control group.

The cosmetic having the skin aging prevention effect according to the present invention is prepared by adding the above-mentioned cosmetic composition in an amount of 0.1 to 50 wt% based on the total weight of the cosmetic.

If the added amount of the cosmetic composition is less than 0.1 wt%, it is difficult to impart the antioxidative functionality through the composition. If the added amount exceeds 50 wt%, there may be a problem in the basic composition of the cosmetic product, The cosmetic composition is preferably limited to a range of 0.1 to 50 wt% with respect to the total weight of the cosmetic.

Specific examples (Examples 13 to 24) of the cosmetic (lotion) produced using the above-described cosmetic composition (Examples 1 to 12) are shown in Table 7 below (unit: wt%).

division Raw material name Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Example 19 Example 20 Example 21 Example 22 Example 23 Example 24 Comparative Example 1











Paid Butlyparaben 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Parsol mcx 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 Cetanol 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 Arlacel165 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Tagocare450 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Meadowfoam Seed oil 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Vitamine E Acetate 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 DC 345 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Salacos99 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Silicone 200F 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00






Awards Methylparaben 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 TEA 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Glycerin 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 EDTA 2Na 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Propylene Glycol 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 DI Water 68.2 68.2 68.2 68.2 68.2 68.2 68.2 68.2 68.2 68.2 68.2 68.2 73.2

Incrementer
Carbopol 940 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 D.I.Water 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00
Spices Spices (D41749) 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Example 1 Cosmetic composition 5.00 0 0 0 0 0 0 0 0 0 0 0 0 Example 2 Cosmetic composition 0 5.00 0 0 0 0 0 0 0 0 0 0 0 Example 3 Cosmetic composition 0 0 5.00 0 0 0 0 0 0 0 0 0 0 Example 4 Cosmetic composition 0 0 0 5.00 0 0 0 0 0 0 0 0 0 Example 5 Cosmetic composition 0 0 0 0 5.00 0 0 0 0 0 0 0 0 Example 6 Cosmetic composition 0 0 0 0 0 5.00 0 0 0 0 0 0 0 Example 7 Cosmetic composition 0 0 0 0 0 0 5.00 0 0 0 0 0 0 Example 8 Cosmetic composition 0 0 0 0 0 0 0 5.00 0 0 0 0 0 Example 9 Cosmetic composition 0 0 0 0 0 0 0 0 5.00 0 0 0 0 Example 10 Cosmetic composition 0 0 0 0 0 0 0 0 0 5.00 0 0 0 Example 11 Cosmetic composition 0 0 0 0 0 0 0 0 0 0 5.00 0 0 Example 12 Cosmetic composition 0 0 0 0 0 0 0 0 0 0 0 5.00 0 Sum 100 100 100 100 100 100 100 100 100 100 100 100 100

[ Test Example 2 ]

The moisturizing effect of the cosmetic lotion (Examples 13 to 24) prepared by containing the cosmetic composition of antioxidation function shown in Examples 1 to 12 and the cosmetic lotion (Comparative Example 2) containing no cosmetic composition was measured.

The subjects were 60 men and women with healthy skin (22 ~ 28 years old) without any history of diseases such as allergic diseases or atopic dermatitis.

The subjects were divided into 12 groups of 5 persons. The first group was the cosmetic lotion of Example 13, the second group was the cosmetic lotion of Example 14, the third group was the cosmetic lotion of Example 15, the fourth group was conducted The cosmetic lotion of Example 16, the cosmetic lotion of Example 16, the cosmetic lotion of Example 16, the cosmetic lotion of Example 16, the cosmetic lotion of Example 16, the cosmetic lotion of Example 16, , The ninth group is the cosmetic lotion of Example 21, the 10th group is the cosmetic lotion of Example 22, the 11th group is the cosmetic lotion of Example 23, the 12th group is the cosmetic lotion of Example 24, The moisturizing effect of the skin was evaluated by the 5-point scale method using lotion. The results are shown in Table 8 below.

Each subject was treated with the same soap, and then the arm was covered with the soap and the test area was stabilized for 20 minutes under constant temperature and humidity conditions (temperature 26 ° C, relative humidity 45%).

division Average moisturizing effect score Example 13 3.2 = [(4 points * 1) + (3 points * 4) / 5] Example 14 3.4 = [(4 points * 2) + (3 points * 3) / 5] Example 15 3.4 = [(4 points * 2) + (3 points * 3) / 5] Example 16 4.2 = [(5 points * 2) + (4 points * 2) + (3 points * 1) / 5] Example 17 4.2 = [(5 points * 2) + (4 points * 2) + (3 points * 1) / 5] Example 18 4.6 = [(5 points * 3) + (4 points * 2) / 5] Example 19 3.4 = [(5 points * 1) + (3 points * 4) / 5] Example 20 3.8 = [(5 points * 2) + (3 points * 3) / 5] Example 21 4.2 = [(5 points * 3) + (3 points * 2) / 5] Example 22 3.4 = [(5 points * 1) + (3 points * 4) / 5] Example 23 3.4 = [(5 points * 1) + (3 points * 4) / 5] Example 24 3.8 = [(5 points * 2) + (3 points * 3) / 5] Comparative Example 2 2.4 = [(3 points * 2) + (2 points * 3) / 5]

(1: very bad, 2: bad, 3: normal, 4: good, 5: very good)

According to the results of the above Table 8, the cosmetic lotions containing the cosmetic composition according to the present invention (Examples 13 to 24) are superior to the cosmetic lotion without cosmetic composition according to the present invention (Comparative Example 2) It can be confirmed that the effect is very excellent.

In particular, it was confirmed that the moisturizing effect of the cosmetic lotion according to Examples 16 to 18 and 21 was better than that of the cosmetic lotion according to the other examples. It can be concluded that it is more preferable to produce a cosmetic lotion comprising the flaxseed extract, the grape seed extract, the waxy extract and the onion skin extract.

The functional anti-aging cosmetic composition using the natural fermented extract according to the present invention enhances useful components contained in pine leaves, spirulina, and medicines through fermentation and has a high antioxidative function through mixing of these ingredients, It is possible to provide a cosmetic having free radical scavenging activity which acts as an important cause and has an antioxidative, antimicrobial, moisturizing and whitening effect by a natural substance, which is highly industrially applicable.

Claims (3)

The pine leaves collected from the shrimp were washed with water,
The surface of the pine needle was washed with water and then cut,
35 to 64.5wt% of cut pine leaves, 35 to 64.5wt% of water and 0.5 to 1.5wt% of sucrose are mixed to prepare a mixture,
The active ingredient in the mixture is eluted by osmotic pressure and fermented at 18 to 25 ° C for 30 to 90 days so that natural fermentation takes place, and 0.1 to 0.5% by weight of sucrose is further added Lt; / RTI >
The pine needle liquor fermentation product obtained through the fermentation process was concentrated under reduced pressure,
1 to 80 wt% of a pine needle-leaf extract (A) prepared by lyophilizing the fermented product under reduced pressure concentration at -65 ° C to -50 ° C and 8 to 15 mmHg;
Spirulina was centrifuged at 2,000 to 3,000 rpm for 10 to 20 minutes to recover the submerged spirulina,
70% ethanol was added to the recovered spirulina in a ratio of 4.5 to 5.5, and the mixture was extracted with stirring at 38 to 42 캜 for 2 to 4 hours to obtain an extract,
After centrifuging the extract at 2,500 to 3,500 rpm for 10 to 15 minutes, a supernatant was obtained. The supernatant was added to the fermenter and the sugar content of the fermenter was adjusted to 20 to 25 ° Brix. Natural fermentation by microorganisms is carried out for 10 to 15 days to obtain a fermented product,
1 to 80% by weight of the spirulina fermentation extract (B) prepared by powder-phase lyophilization under the conditions of -65 ° C to -50 ° C and 8 to 15 mmHg after concentrating the fermented product under reduced pressure;
90 to 96% by weight of distilled water and 4 to 10% by weight of a medicament are mixed and then bath for 3 to 5 hours to prepare a bath liquid. The bath liquid is prepared by mixing 85 to 90% by weight of the bath liquid, 5 to 10% After natural fermentation for 11 days, the sugar was removed through precipitation filtration using distilled water and ethanol,
The liquid fermentation product of the drug substance from which sugar was removed was concentrated under reduced pressure,
(A + B + C) of 1 ~ 80wt% of the fermented extract (C) prepared by powder lyophilizing the fermented product under reduced pressure at -65 ℃ ~ -50 ℃ and 8 ~ 15mmHg As a result,
The medicinal material may be selected from the group consisting of Terminalia chebula 0.1 to 30 wt%, Alpinia officinarum 0.1 to 30 wt%, Sophora japonica 0.1 to 30 wt%, Salviae miltiorrhizae 0.1 to 30 wt%, Sorbus commixta 0.1 (Caesalpinia sappan), Zanthoxylum schinifolium (0.1 to 30 wt%), Phenolic acid (0.1 to 30 wt%), Portulaca oleracea (0.1 to 30 wt.%), Paeonia lactiflora (0.1 to 30 wt.%), Areca catechu 0.1 to 30 wt% of Epimedium koreanum, 0.1 to 30 wt% of Syzygium aromaticum, 0.1 to 30 wt% of Lycium chinense, 0.1 to 30 wt% of Sanguisorba officinalis, 0.1 to 30 wt% of Taraxacum platycarpum, 0.1 to 30 wt% of Carthamus tinctorius, 0.1 to 30 wt% of Puerariae thunbergiana, 0.1 to 30 wt% of Cimicifuga heracleifolia, Scutellaria baicalensis And 0.1 to 30 wt% of Golden thread, 0.1 to 30 wt% of licorice (Glycyrrhiza uralensis Fisch), and 0.1 to 30 wt% of Cinnamonum cassia Presl. Using anti-aging natural fermentation extract functional cosmetic composition.
The method according to claim 1,
Wherein the cosmetic composition is prepared by adding one of the following ingredients 1, 2, or 3 to the total weight of the cosmetic composition.
1. 0.1 ~ 0.5wt% of flaxseed extract, 0.1 ~ 0.5wt% of grape seed extract, 0.1 ~ 0.5wt% of salted extract, 0.1 ~ 0.5wt% of onion skin extract,
2. Spolassis crispa extract 0.1 ~ 0.5wt%, Phellinus linteus extract 0.1 ~ 0.5wt%, Inonotus obliquus extract 0.1 ~ 0.5wt%
3. Capsiphon fulvescens extract 0.1 ~ 0.5wt%, Enteromorpha intestinalis extract 0.1 ~ 0.5wt%, Undaria pinnatifida extract 0.1 ~ 0.5wt%
The cosmetic composition according to claim 1, wherein the cosmetic composition is prepared by adding 0.1 to 50 wt% of the cosmetic composition according to claim 1.