KR20180042626A - Composition comprising bee venom for relieving hangover - Google Patents

Abstract

The present invention relates to a composition comprising bee venom for relieving a hangover, wherein the composition comprising the bee venom has an effect of increasing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) to be excellent in relieving hangover after drinking, thereby being easily used as health functional food for reliving hangover comprising the same.

Description

[0001] Composition comprising bee venom for relieving hangover [

The present invention relates to a composition for hangover decomposition containing bee venom, and more particularly to a bee venom of bees having an effect of increasing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase To a composition for spoilage.

Drinking has become an important social life culture that solves the role of stress relief while acting as an essential bridge to human relationships for those living in modern times. Excessive drinking beyond the right line, however, will cause a hangover, such as a headache, sickness or vomiting, even the next day. Most of these phenomena are caused by the toxic action of alcohol or acetaldehyde accumulated in hepatocytes without decomposition, and as these substances accumulate, the fatigue persists or the abdominal bloom is felt.

As the alcohol is absorbed through the stomach and small intestine, the alcohol component is transported through the blood vessels to reach the liver. The hepatocyte is converted into acetaldehyde (alcohol) by using alcohol dehydrogenase (ADH) And acetaldehyde is again decomposed into acetic acid through acetaldehyde dehydrogenase in hepatocytes and finally decomposed into carbon dioxide gas and water. In this process, acetaldehyde and alcohol that are not 100% oxidized cause hangover phenomena such as helplessness and fatigue.

Therefore, various hangover products are pouring out in order to solve this phenomenon, and drinks or drinks are most developed as hangover and small-sized products. In recent years, medicines, food, and beverages containing a major component such as hinoki ginseng, ginseng, red ginseng, corn, barberry, honey, vitamins, soybean, 칡, dermis, green tea, bean sprouts, But it has been confirmed that the hangover resolution effect of the above compositions is not very high. In addition, since most of these plant materials have been traditionally used for hangover resolution, There is little research on the composition for hangover and sputum.

There are four species of bees: Apis mellifera , Apis cerana , Apis dorsata , Apis florea , and so on. At present, there are western and oriental bees in Korea. Especially, oriental bees have been reared since the Goryeo Dynasty, and have been used as a needle therapy in the private sector and in the oriental medicine. However, the western varieties of bees were introduced into Korea in 1892 by German missionaries during the Emperor Gojong period of Joseon Dynasty. Bee venom is contained in the bee venom of the bee, which is composed of about 40 substances and has been used for the treatment of diseases of the human body since BC. The bee venom shows differences in its components depending on the bees, and even in the same bee, there is a difference in composition depending on the age. Currently, studies on the action and mechanism of bee venom component, sensitivity and toxicity of bee venom, immunotherapy and treatment of diseases such as arthritis, herpes simplex, multiple sclerosis and tumor have been reported. Especially, It is known.

The present inventors have studied various physiological activities of tablet bee venom and have found that the tablet bee venom has an effect of increasing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase, And the present invention was completed.

Korean Patent No. 10-0706465 (Title of the Invention: Composition of Mixed Drink for Hangover Solution, Applicant: Taeyoung Lee & Co., Inc., Registered Date: April 04, 2007) Korean Patent Laid-Open No. 10-2013-0092653 (Name of the invention: natural tea for hangover and its preparation method, applicant: Wang Yong Zhong, publication date: Aug. 21, 2013)

It is an object of the present invention to provide a composition for hangover poultry containing bee venom.

The present invention relates to a composition for dead hangover containing bee venom.

The bee venom can be a purified rod isolated from a bee venom of the bees ( Apis mellifera L.). At this time, it is preferable that the worker bees are at least 15 days old.

The present invention can also provide a composition for hangover decoy comprising a composition containing bee venom to which at least one extract selected from the group consisting of Hovenia dulcis extract, asparagus extract, and bean sprouts root extract is added.

The present invention also relates to a health functional food for hangover habit characterized by comprising a composition containing bee venom.

The health functional food is characterized in that it is selected from various drinks, meat, sausage, bread, candy, snack, noodle, ice cream, dairy product, soup, ionic drink, beverage, alcoholic beverage, gum, tea and vitamin complex.

The bee venom concentration in the health functional food having a liquid formulation is preferably 0.5 to 10 mg / ml. And preferably 0.5 to 5.0 mg / ml. More preferably 0.5 to 1.5 mg / ml. Most preferably 1.0 mg / ml.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for dead hangover containing bee venom. The bee venom may be a purified rod isolated from a gall bladder of a beeswax ( Apis mellifera L.) bees, and it is preferable that the bees be at least 15 days old.

The present invention can also provide a composition for hangover decoy comprising a composition containing bee venom to which at least one extract selected from the group consisting of Hovenia dulcis extract, asparagus extract, and bean sprouts root extract is added. Bee venom is mixed with Hovenia dulcis extract, Asparagus extract, Bean root root extract, etc.

The extract may be extracted with water, C1-C4 alcohol or a mixture thereof. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. The water, the C1-C4 alcohol, or the mixed solution thereof used in the preparation of the extract may be 1 to 40 times by volume (1 to 40 liters per 1 kg) of the erythroplumoformy used, preferably 5 to 40 Boat volume can be used. The extraction conditions of the extract may be from 20 to 120 ° C for 1 minute to 48 hours. The above process can be repeated 1 to 4 times. As an apparatus for extracting the extract, a conventional extracting apparatus, an ultrasonic pulverizing extractor or a fractionator may be used. The thus prepared extract can be removed by hot air drying, vacuum drying or freeze drying. The above-mentioned extract can be isolated or isolated by known methods used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), hexane and water, And may be fractionated or purified using a combination method. The chromatography can be carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The composition for spoiling hangover of the present invention can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, oral formulations, external preparations, suppositories and sterilized injection solutions according to a conventional method have. Examples of carriers, excipients and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as starch, calcium carbonate, sucrose or lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The dosage of the composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection. Since the composition of the present invention has little toxicity and side effects, it can be safely used for prolonged use for preventive purposes.

In addition, the present invention provides a health functional food for hangover marine including the above-described hangover remedy composition and a food-acceptable food supplementary additive. At this time, bee venom can be added to the health functional food of the present invention in an amount of 0.001 to 100% by weight. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various kinds of drinks, meat, sausage, bread, Snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamin complexes.

When the formulation is a liquid in the health functional food, the bee venom concentration is preferably 0.5 to 10 mg / ml. And preferably 0.5 to 5.0 mg / ml. More preferably 0.5 to 1.5 mg / ml. Most preferably 1.0 mg / ml. At this time, if the bee venom concentration is less than 0.5 mg / ml, the effective ingredient in the bee venom changes and the hangover resolution effect may be changed. If the bee venom concentration is more than 10 mg / ml, the effective ingredient in bee venom may not be stable. The health functional food having a liquid form can be a drink, an ice cream, a dairy product, a soup, an ionic drink, a drink, an alcoholic beverage, tea, and the like. At this time, it is preferable that the mixed concentration of the hinoki extract, the asparagus extract, and the bean root root extract is also 0.5-5.0 mg / ml.

The present invention relates to a composition for hangover decomposition containing bee venom, wherein the bee venom-containing composition has an effect of increasing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase, It is excellent in hangover relief and can be easily used as a health food for hangover, which contains it. Korean Patent No. 10-0706465 and Korean Patent Laid-Open No. 10-2013-0092653 disclose a composition for hangover marine including honey. However, it has not been disclosed that bee venom has a hangover resolution effect.

1 is a photograph showing a process of collecting bee venom using a bee venom collecting device.
FIG. 2 is a chromatogram showing the distribution of the bee venom components by liquid chromatography analysis of the capsular bag by the ages of bees.
FIG. 3 is a graph showing the bee venom contents in the viscus by liquid chromatography analysis of the viscus according to the age of bees.
Fig. 4 is a photograph (left side of Fig. 4) (left side of Fig. 4) and a photograph (right side of Fig. 4) of the tablet bean poison beet collected from a 15- .
FIG. 5 is a chromatogram showing the results of confirming whether the bean sprouts used as the bee venom reference agents, afalmine, phospholipase A2, adolapine, hyaluronidase and melittin are present in purified bee venom using ultra high performance liquid chromatography (UPLC).
6 shows the results of measuring the activity of alcohol dehydrogenase (ADH) in tablet bee venom.
FIG. 7 shows the results of measuring the activity of acetaldehyde dehydrogenase in tablet bee venom.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Preparation of tablet bee venom &

Collecting bee venom from bee venom using bee venom collecting device of western bees ( Apis meliffera L.), bees with an adult time of less than 1 hour, worker bees of 3 to 8 days, worker bees of 9 to 14 days, Respectively, and purified bee venom dried beverages with pure water solubility were prepared by removing saccharides and volatile substances through a simple purification method of bee venom. FIG. 1 shows a bee venom collecting process using such a bee venom collecting device.

On the other hand, when the bee venom contents of the bee venom of each day were checked, as shown in Fig. 2 and Fig. 3, the pure bee poison content was the highest in the tourniquet of the bees of more than 15 days old. Therefore, in the present invention, tablet bee venom collected from worker bees over 15 days of age will be used for the experiment.

≪ Example 2: Ultra high performance liquid chromatography analysis for confirming the components of tablet bee venom &

40 mg of purified bee venom collected from 15-day old or older bees was dissolved in 10 ml of distilled water and filtered through a 0.2 μm PTFE filter. The standard of apamin, melittin, phospholipase A2, adalapine and hyaluronidase Dissolved in tea distilled water to make 2 mg / ml and prepared by filtration using the same filter as the test bee venom. Analysis of the bee venom components was carried out by ultra high performance liquid chromatography (UPLC). Analytical instruments were the I class model of Waters Company and Halo peptide ES-C18 (particle size: 2.7 μm, inner diameter: 4.6 mm, Length: 10 cm). As the mobile phase, a solution A: a mixed solution obtained by mixing an acetonitrile (MeCN) solution of 20 mM trifluoroacetic acid (TFA) and an aqueous solution of B solution: 20 mM trifluoroacetic acid (TFA) was used. When the migration time is less than 3 minutes, the solution A is less than 31% by volume and when the migration time is less than 3 minutes and less than 5 minutes, the solution A is less than 40% by volume and the migration time is 5 Min and less than 10 minutes, the solution A was mixed with the solution B so that the volume ratio of the solution A was not less than 40 vol.% And not more than 45 vol.%. The flow rate of the mobile phase was 1.5 ml / min, the injection amount of the sample was 4 μl, and the wavelength of the ultraviolet absorptiometer was set at 220 nm. The temperature of the column was adjusted to 50 ° C during the analysis. The detection limits were calculated according to the ICH guidelines (International Conference on Harmonization Guideline). The above conditions are shown in detail in Table 1 below.

device Waters I class column Halo ES-18 (4.6 x 100 mm, 2.7 [mu] m) Column temperature 50 ℃ Flow rate 1.5 ml / min Volume 4 μl Detection wavelength 220 nm This phase (A) 20 mM TFA / MeCN, (B) 20 mM TFA / H ₂ O
(A) 0-3 min: 10-31 vol% / 3-5 min: 31-40 vol% / 5-10 min: 40-45 vol%

The analytical results of the thus-analyzed aqueous solutions of purified bee venom were shown in FIG. 5 through comparison of peaks with standard solutions, and the content of ampholytic, melittin, phospholipase A2, adolapine and hyaluronidase Respectively.

ingredient content(%) Apamin 2.3 Melitin 63.6 Phospholipase A2 11.5 Hyaluronidase 1.9 Adolapine 0.9

<Example 3> Measurement of alcohol dehydrogenase (ADH) activity of purified bee venom>

Activity of ADH (alcohol dehydrogenase) activity was measured using an ADH ELISA kit (Biocompare). For this purpose, 96 wells were injected with positive control and purified bee venom (collected from 15-day-old or older beetles), and the absorbance was measured at 340 nm for 6 hours. The increase rate of ADH activity was measured based on the negative control 6. The positive control used in the ADH ELISA kit.

Referring to FIG. 6, it was confirmed that the tablet bee venom significantly increased ADH activity above the concentration of 0.5 mg / ml. Although not shown in FIG. 6, tablet bee venom appears to have a stable ADH activity increasing effect up to a concentration of 10 mg / ml.

<Example 3> Measurement of acetaldehyde dehydrogenase (ALDH) activity of purified bee venom>

ALDH (acetaldehyde dehydrogenase) activity was measured using an ALDH ELISA kit (Biocompare). For this, 96 wells were injected with positive control and purified bee venom (collected from 15-day-old or older beetles), and the absorbance was measured at 340 nm for 6 hours. The ALDH activity increase rate was measured based on the negative control 7. At this time, the positive control used in the ALDH ELISA kit was used.

Referring to FIG. 7, it was confirmed that the serum bee venom significantly increased the ALDH activity above the concentration of 0.5 mg / ml. Although not shown in FIG. 7, tablet bee venom appears to have a stable ALDH activity increasing effect up to a concentration of 10 mg / ml.

Example 5. Confirmation of Blood Alcohol Degradation Effect < RTI ID = 0.0 >

In order to measure the alcohol degradation effect of purified bee venom collected from 15 - day - old and older work bees, 30 healthy adult men and women over 20 years of age were studied. The specific characteristics (average value) of the subject of the clinical experiment are shown in Table 3 below, and the blood alcohol concentration is shown in Table 4 below. For the experiment, 300 ㎖ of soju was drunk for 20 minutes, and 3 tablets of bean sprouts per 1 cup of soju were drunk for 20 minutes. Ml). In addition, a group of 10 ㎖ mixed liquids containing 1 ㎎ / ㎖ of tablets and 4 ㎎ / ㎖ of Hodgkin 's hot - water extract, asparagus hot - water extract and bean sprout root hydrothermal extract, Respectively. There was a bee venom / extract-free group in the control group and a group (100 ml) of morning-care sold in the market instead of bee venom / extract. After 90 minutes of drinking, the alcohol concentration was measured using a breathalyzer.

division range Age (age) 30 years old Gender Male Female) 2: 1 weight 70.9

Condition After 90 minutes of drinking
Blood alcohol concentration (%) Tablet bee venom (1 mg / ml) 0.079 Tablet bee venom (5 mg / ml) 0.043 Tablet bee venom (1 mg / ml) +
Houttuynia cordata extract (4 mg / ml) 0.021 Tablet bee venom (1 mg / ml) +
Asparagus hot water extract (4 mg / ml) 0.035 Tablet bee venom (1 mg / ml) +
Soybean sprout root hydrothermal extract (4 ㎎ / ㎖) 0.018 Houttuynia cordata extract (5 mg / ml) 0.213 Asparagus hot water extract (5 mg / ml) 0.221 Soybean sprout root hydrothermal extract (5 mg / ml) 0.224 Non-intake group 0.231 Morning care 0.195

Referring to the results of Table 4, it can be confirmed that the blood alcohol reduction rate is excellent when the composition for a hangover remedy according to the present invention is used. Especially, when the beverage containing Houttuynia cordata hydrothermal extract, Asparagus hot-water extract, and bean sprout root hydrothermal extract was added to tablet bee venom, these effects were more excellent.

Example 6: Confirmation of hangover resolution effect [

In order to evaluate subjective symptoms on the day following drinking, the subjects who performed the experiment of Example 5 were classified into the items of hangover elimination effect, digestive effect, and headache degree as "no effect" as 1 and " (1 to 5), and the results are shown in Table 5 below.

Condition Hangover removal effect Degree of digestion Degree of headache reduction Tablet bee venom (1 mg / ml) 3.7 3.8 3.5 Tablet bee venom (5 mg / ml) 3.9 4.0 3.9 Tablet bee venom (1 mg / ml) +
Houttuynia cordata extract (4 mg / ml) 4.3 4.2 4.1 Tablet bee venom (1 mg / ml) +
Asparagus hot water extract (4 mg / ml) 4.3 4.5 4.0 Tablet bee venom (1 mg / ml) +
Soybean sprout root hydrothermal extract (4 ㎎ / ㎖) 4.2 4.1 4.2 Houttuynia cordata extract (5 mg / ml) 1.6 1.6 1.9 Asparagus hot water extract (5 mg / ml) 1.9 1.6 1.6 Soybean sprout root hydrothermal extract (5 mg / ml) 1.7 1.9 1.7 bottled water 1.0 1.1 1.1 Morning care 2.5 2.4 2.4

As shown in Table 5, it was confirmed that the tablets of the present invention had the highest hangover resolution effect. In addition, it can be seen that the purified bee venom drink containing the hydrothermal extract of Hovenia dulcis, the hydrothermal extract of Asparagus, and the hydrothermal extract of the bean sprouts has a better hangover removal effect.

&Lt; Formulation example 1. Food production >

Formulation Example 1-1. Manufacture of cooking seasonings

Tablet bean poison taken from a bee of a Western species of bees aged 15 days or more in Example 1 of the present invention was added to the cooking seasoning at 1 wt% to prepare a cooking sauce for health promotion.

Formulation Example 1-2. Manufacture of flour food products

The bee venom collected from the bees of the western species of bees aged 15 days or more in Example 1 of the present invention was added to flour at 0.1 wt% and bread, cake, cookies, crackers and noodles were prepared using this mixture to prepare health promotion foods .

Formulation Example 1-3. Manufacture of soups and gravies

The health enhancing soup and the juice were prepared by adding 0.1% by weight of purified beacon collected from the bees of the western species of bees aged 15 days or more in Example 1 of the present invention to the soup and the juice.

Formulation Examples 1-4. Manufacture of dairy products

The bee venom collected from the bees of the western species of bees aged 15 days or more in Example 1 of the present invention was added to milk at 0.1 wt%, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 1-5. Vegetable juice manufacturing

The vegetable juice for health promotion was prepared by adding 0.5 g of tablets bee venom collected from the bees of the western species of bees aged 15 days or more in Example 1 of the present invention to 1,000 ml of tomato juice or carrot juice.

Formulation Example 1-6. Manufacture of fruit juice

Health enhancing fruit juice was prepared by adding 0.1 g of purified bee venom collected from a worker bees of a Western species of bees aged 15 days or more in Example 1 of the present invention to apple juice or 1,000 ml of grape juice.

Formulation Example 1-7. Manufacture of tablets

200 g of purified bee venom collected from the bees of the western species of bees aged 15 days or more in Example 1 of the present invention were mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

Claims (7)

A composition for spit hunting characterized by containing bee venom. The method according to claim 1,
Wherein the bee venom is a tablet bee venom isolated from a bee venom of a beeswax ( Apis mellifera L.) worker bee. 3. The method of claim 2,
Wherein said worker bees are at least 15 days old. The composition of claim 1, wherein at least one extract selected from the group consisting of Hovenia dulcis extract, asparagus extract, and bean sprouts root extract is added to the composition of claim 1. A health functional food for hangover habit, comprising the composition of claim 1 or 4. 6. The method of claim 5,
Wherein the health functional food is selected from a variety of drinks, meats, sausages, breads, candies, snacks, noodles, ice creams, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamins Health functional foods. 5. The method of claim 4,
Wherein the bee venom concentration in the health functional food having a liquid formulation is 0.5 to 10 mg / ml.

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