JP2007051103A - Antioxidant composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition having antioxidant effect able to be used for wide range of foods and drinks and pharmaceuticals, as antioxidant materials are effectively ingested for scavenging active oxygen and SOD (Super Oxide Dismutase) can be supplied in case of SOD insufficiency in the body, but it is difficult to quickly absorb SOD even through orally ingested and SOD can be deactivated through decomposition caused by gastric acid, so it is an effective means to ingest an antioxidant material expressing the same activity as that of SOD. <P>SOLUTION: The antioxidant composition comprises fruits or seeds of Xanthium strumarium or an extract of the same. The extract of fruits or seeds of Xanthium strumarium is prepared by using at least one species of a group consisting of water, bases, acids, hydrophilic solvents and acetone. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an antioxidant composition containing fruit, seeds or an extract thereof, and food / beverage products, quasi-drugs and pharmaceuticals containing the same.

  Humans breathe constantly and take oxygen into their bodies. Approximately 2% to 4% of oxygen ingested by breathing is an active oxygen with unstable unpaired electrons (those with one or more unpaired electrons in the molecule are called free radicals, and oxygen free radicals are active oxygen. These active oxygens attack cell membranes for stability in an attempt to stabilize quickly. Thereafter, the unsaturated fatty acid in the cell membrane is oxidized, and the oxidized unsaturated fatty acid is converted into a lipid peroxyl radical. The lipid peroxyl radical again oxidizes unsaturated fatty acids and generates lipid peroxides while repeating oxidation. Although active oxygen also has a role of protecting itself from bacteria that have invaded the body, the problem is when a large amount of active oxygen is generated. Active oxygen is harmful because unsaturated fatty acids are oxidized by active oxygen in the body to become “lipid peroxide”. The human body is composed of approximately 60 trillion cells, and the outermost cell membrane is made of “unsaturated fatty acids”. When the unsaturated fatty acid is oxidized to become lipid peroxide and the cell membrane becomes tattered, even proteins in the cell membrane are involved and oxidized to form a more harmful secondary oxide. As a result, various diseases such as aging, stroke, autoimmune disease, cancer, cataract, fatty liver, myocardial infarction, ischemic heart disease, Alzheimer syndrome, diabetes, diabetic nephropathy, arteriosclerosis, allergy, hay fever and Aging such as skin wrinkle formation and skin elasticity decrease, inflammation, and skin pigmentation are caused. Fortunately, however, the human body is equipped with antioxidants, and it contains antioxidants such as SOD (superoxide dismutase), catalase and glutathione peroxidase in the body. Give me. However, this SOD also decreases with age. Moreover, due to many environmental pollutants such as recent agricultural chemicals, insecticides, food additives, nitrogen compounds, ultraviolet rays, radiation, stress, tobacco, environmental pollution only consumes precious SOD in our bodies. There is no environment where active oxygen is made.

  In order to eliminate active oxygen, it is only necessary to take an antioxidant, and if SOD in the body is insufficient, SOD may be supplemented, but it is difficult to absorb SOD quickly even if taken orally, In addition, it is decomposed into stomach acid and loses its activity. Therefore, taking an antioxidant substance exhibiting the same activity as SOD is an effective means.

  Examples of the antioxidant include water-soluble antioxidant compounds such as L-ascorbic acid (vitamin C), reduced glutathione, uric acid, polyphenols, α-tocopherol (vitamin E), ubiquinone (coenzyme Q), and carotenoids. And the like, and synthetic compounds such as BHT (butylhydroxytoluene) and BHA (butylhydroxyanisole) are known. However, synthetic antioxidants BHT and BHA have problems such as suspected carcinogenicity. Considering the daily intake of antioxidants, foods are mainly used as raw materials in the production of antioxidants because of their economics and safety. For example, polyphenols such as catechins from tea leaves and procyanidins from red wine Kinds are manufactured. As an antioxidant as other natural ingredients, an extract selected from plum, rapeseed, bengal karatachi, miracle berry, yaba santa, lemongrass (for example, refer to Patent Document 1), agaricus, mesimacob, and propolis ( For example, refer to Patent Document 2), spikelet extract (for example, refer to Patent Document 3), salamander aqueous solvent extract (for example, refer to Patent Document 4), cacao leaf, bark or root extract (for example, , Patent document 5), extract of bran, germ or flour with organic solvent (for example, refer to patent document 6), vegetables that are said to be nutritionally superior, pumpkin, spinach, morroheia, tomato, What consists of 15 or more types of natural food materials, such as sweet corn, carrot, purple corn, is known (for example, refer patent document 7). On the other hand, it has been found that ingesting various and various antioxidants in small amounts is more effective in preventing various diseases than ingesting large amounts of a single substance. For that purpose, it is necessary to provide various antioxidants.

JP 2003-183120 A (page 1-2) JP 2002-235084 A (page 1-2) JP 2002-371092 A (page 1-2) JP 2002-104985 A (page 2-3) JP 2000-60485 A (page 1-2) JP-A-10-147779 (page 1-2) JP-A-10-75740 (page 2-5)

  An object of the present invention is to provide a composition having an antioxidant effect that can be used for a wide variety of foods and drinks and pharmaceuticals, and foods and drinks, quasi drugs and pharmaceuticals containing the same.

  As a result of diversified research and studies for the purpose of searching for antioxidant components using various natural plants, the present inventors have demonstrated excellent antioxidative action and SOD-like activity in fruit, seeds or extracts thereof. The present invention was completed.

  Antioxidant composition containing fruit, seed or extract thereof of onam fir obtained in the present invention is a measurement result of DPPH (diphenylpicrylhydrazyl) free radical scavenging ability using an electron spin resonance (ESR) apparatus Similarly, SOD-like activity measurement results and streptozotocin (STZ) obtained by a method in which reactive oxygen species generated by an appropriate method are captured by a spin trap agent such as DMPO and an ESR signal specific to each reactive oxygen species is quantified. A substance that reacts with thiobarbituric acid (TBA) on lipid peroxide, which increases in vivo due to oxidation, by administration of an antioxidant composition to diabetic nephropathy rats induced by administration and addition test to fish meat ( From the result of measurement with the amount of thiobarbituric acid-reactive substance (TBARS), it was found that the antioxidant effect was high.

  The present invention uses an antioxidant composition containing a fruit of onamomi or an extract thereof for various foods and medicines, aging, stroke, autoimmune disease, cancer, cataract, fatty liver, myocardial infarction, ischemic Can suppress the progression of various diseases such as heart disease, Alzheimer's syndrome, diabetes, diabetic nephropathy, arteriosclerosis, allergy, hay fever and abnormal pigmentation such as stains and freckles, and the deterioration of food .

  The onamomi used in the present invention is called scientific name: Xantium Strumaria L. It is an annual plant belonging to the genus Onamomi and has a short bristle. Seeds alternate on stems, are almost heart-shaped, sharp at the tip, cut irregularly at seed edges, thicker, slightly harder, and short haired. In summer, yellow-green head-shaped flowers are attached in cones to the branches. Male flowers have a stiff green sting at the top and female flowers at the bottom. The fruit remains wrapped in a full bowl, has a sting around it, and is spread on clothes and animal hair. There are about 20 species of onamomi in the world, but the origin is the Asian continent, which is widely distributed in the world, but especially in the Americas. The ancient name was used as a dye to dye hair yellow, and the genus name is Xanthos, which is derived from the Greek word yellow. ) ”. It is also called Cooklebur in English. Herbal matured fruit is a herbal medicine, called a mushroom, and is used as an antipyretic, sweating, and headache medicine. In Europe and North Africa, it has been used as livestock feed and as a medicine to remove lymph gland swelling.

  In the present invention, fruits or seeds are used as the ona fir part. The form is not particularly limited, and any of immature fruit, fully-ripe fruit, dried fruit, seed powder, and the like may be used.

  In the case of using a product containing a water-insoluble component such as fresh fruit or dried fruit, it is preferable that the water-insoluble component is removed by extraction in order to increase the effect.

  In the case of using fresh fruits during extraction, it is preferable to use water that has been crushed and homogenized with a mixer or the like in order to increase extraction efficiency with or without the addition of water.

  When using a dried fruit, it is preferable to grind | pulverize so that it may become a particle size of 40 mesh or less, in order to improve extraction efficiency.

  The extraction method is not particularly limited, such as extraction solvent and extraction temperature, and water, base, acid, hydrophilic solvent, and acetone can be used as the extraction solvent. The hydrophilic solvent is preferably one or more selected from the group of lower alcohols such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, and butyl alcohol from the viewpoint of operability and extraction efficiency. Particularly preferred is water, base, or acid.

  When using an acid or a base as the extraction solvent, it is preferable to neutralize the extract. The salt produced by the neutralization reaction can be removed by a known method such as dialysis or gel filtration. When water is used as the extraction solvent, the neutralization reaction as described above is not necessary, and it is not necessary to remove the generated salt. Therefore, it is more preferable to use water.

  The acid used at this time is not particularly limited, and most of the acid can be used. However, it is preferable to use one kind or a combination of both selected from hydrochloric acid and sulfuric acid because of easy availability and operability. is there.

  Further, the base is not particularly limited, and most of the bases can be used, but preferably one kind selected from sodium hydroxide and potassium hydroxide or a combination of both.

  The concentration of the acid or base used for the extraction is not particularly limited before or after the extract is treated with the enzyme, and varies depending on the strength of the acid or base. From the viewpoint of efficiency, it is preferable to use a concentration of 0.01 to 0.5 mol.

In the above extraction, enzymes such as pectinase, protease, tannase or cellulase can be used alone or in combination of two or more.
Further, in the above extraction, it is preferable to repeat the extraction step once or more again on the extraction residue, because the extraction efficiency is improved and the yield is improved. The solvent used for extraction in this case may be the same, or another solvent may be used.

  The above fruit or seed extract can be used as it is, but it is preferable because the antioxidative effect is enhanced and the application range is widened by removing insoluble substances and solvent by filtration, centrifugation and fractional distillation.

  After removing the insoluble substance and the solvent, the extract is directly or concentrated and then distributed using an organic solvent to obtain each solvent-soluble fraction. These solvent-soluble fractions are preferable because the antioxidant effect (SOD-like activity) is further increased. As the organic solvent, methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, lower alcohol of butyl alcohol, ethyl acetate, butyl acetate, diethyl ether, methyl ether, methyl isobutyl ketone, hexane, acetone or chloroform can be used. Of these, ethyl alcohol is preferred. In order to increase the purity of the soluble fraction, partitioning with other hydrophobic solvents can be combined. The concentration of these solvents is not particularly limited, but the final concentration is preferably 10 to 100% from the viewpoint of yield and effect.

  In order to further increase the purity, it is also possible to use a hydrophobic resin based on phenol, styrene, acrylic acid, epoxyamine, pyridine, methacryl or the like. In that case, as an eluent after resin adsorption, lower alcohols such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, butyl alcohol, and acetone can be used alone or as an aqueous solution.

  The extract and fraction can be used as they are, but if necessary, they can be used after being dried and powdered by means such as spray drying or freeze drying.

  The antioxidant effect can be confirmed by measuring free radical scavenging ability and lipid peroxide in blood. The measurement of free radical scavenging ability is carried out by EDP, chemical method such as cytochrome C reduction method, nitrous acid method, nitro blue tetrazolium salt reduction method (NBT reduction method), DPPH radical (diphenylpicrylhydrazyl radical). ) The inhibition rate, superoxide radical scavenging ability, SOD-like activity value, and hydroxy radical value can be confirmed by measurement.

  The antioxidant composition of the present invention can be applied to foods and drinks, pharmaceuticals, feeds and the like, and preferably foods and drinks, quasi drugs and pharmaceuticals that can be easily consumed by humans.

  The food and drink in the present invention is not particularly limited as long as it is a form that can be taken orally, such as a solution, suspension, powder, or solid molded product. More specifically, instant noodles, retort foods, canned foods, microwave foods, instant soups and miso soups, freeze-dried foods, soft drinks, fruit juice drinks, vegetable drinks, soy milk drinks, coffee drinks, tea drinks Beverages such as powdered beverages, concentrated beverages, nutritional beverages, alcoholic beverages, bread, pasta, noodles, cake mixes, flour products such as fried flour and bread crumbs, rice cakes, caramel, chewing gum, chocolate, cookies, biscuits, cakes, Sweets such as pies, snacks, crackers, Japanese sweets, desserts, sauces, processed tomato seasonings, flavor seasonings, cooking mixes, sauces, dressings, soups, curry and stew seasonings, processing Fats and oils such as butter, margarine and mayonnaise, milk beverages, yogurts, lactic acid bacteria beverages, ice creams, chestnuts Agricultural processing of dairy products such as mussels, frozen foods, processed fishery products such as fish ham and sausages, marine products, processed livestock products such as livestock ham and sausages, canned agricultural products, jams and marmalades, pickles, boiled beans and cereals Products, nutritional foods, tablets, capsules and the like.

  The intake amount of the antioxidant composition of the present invention as a food or drink should be adjusted according to the disease state of the present invention, the body weight, age, constitution, physical condition, etc. of the sick person, but in general, the antioxidant composition per day The product can be appropriately selected in the range of 0.05 g to 20 g, preferably 0.1 g to 5 g. This can be taken one to several times a day depending on the state of the disease and the form of food.

  In this invention, when processing into an antioxidant composition or food-drinks, various nutrient components can be strengthened.

Nutritional ingredients that can be enhanced include vitamins such as vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin D, vitamin E, niacin (nicotinic acid), pantothenic acid, folic acid, Essential amino acids such as lysine, threonine and tryptophan, minerals such as calcium, magnesium, iron, zinc and copper, and for example, α-linolenic acid, EPA, DHA, evening primrose oil, octacosanol, casein phosphopeptide (CPP), Casein calcium peptide (CCP), water-soluble dietary fiber, insoluble dietary fiber, substances that contribute to human health, such as oligosaccharides, and one or more useful substances approved as other foods and food additives Can be used.

  The quasi drugs and pharmaceuticals in the present invention can be prepared as oral preparations or injections according to conventional methods using excipients and other additives suitable for oral or parenteral administration. Preferred is an oral preparation, and most preferred is an oral solid preparation that can be easily taken and is easy to store and carry.

  As oral solid preparations, tablets, powders, fine granules, granules, capsules, pills, sustained-release preparations and the like are used. In such solid preparations, appropriate pharmacologically acceptable carriers, excipients (eg starch, lactose, sucrose, calcium carbonate, calcium phosphate etc.), binders (eg starch, gum arabic, carboxymethylcellulose, Mixed with hydroxypropylcellulose, crystalline cellulose, alginic acid, gelatin, polyvinylpyridone, etc.), lubricant (eg, stearic acid, magnesium stearate, calcium stearate), disintegrating agent (eg, carboxymethylcellulose, talc, etc.), etc. Tablets, powders, fine granules, granules, capsules, pills, sustained release agents, etc. can be prepared by the method. Oral liquid preparations include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, elixirs, etc., and generally used inert diluents such as purified water and ethanol. . In addition to the inert diluent, the composition may contain adjuvants such as wetting agents and suspending agents, sweeteners, flavors, fragrances and preservatives.

  Examples of injections for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of the aqueous solution and suspension diluent include distilled water for injection and physiological saline. Examples of diluents for water-insoluble solutions and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, alcohols such as ethanol, polysorbate 80, and the like. Such compositions may further contain adjuvants such as preservatives, wetting agents, emulsifiers, dispersants, stabilizers (eg lactose), solubilizers (eg glutamic acid, aspartic acid). These are sterilized by, for example, filtration through a bacteria storage filter, blending with a bactericide or irradiation. These can also be used by producing a sterile solid composition and dissolving it in sterile water or a sterile solvent for injection before use.

  The dosage of the antioxidant composition of the present invention as a pharmaceutical agent is appropriately determined according to individual cases in consideration of the administration route, disease symptoms, age of the administration subject, sex, etc. The active ingredient is about 40 mg to 3 g / day, preferably 100 to 500 mg / day.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, the following Example does not limit the scope of the present invention.

(Example 1) Preparation 1 of an antioxidant composition
The dried fruit of onamomi was pulverized to 40 mesh or less, 3 L of distilled water was added to 150 g of the powder, and extracted at 100 ° C. for 3 hours. Then, it centrifuged (8500 rpm, 10 minutes), the supernatant was filtered, and the extract and the residue were isolate | separated. The residue was concentrated under reduced pressure, and then freeze-dried to obtain 29.8 g of the antioxidant composition A of the present invention.

(Example 2) Preparation 2 of an antioxidant composition
The dried seeds of onamomi were pulverized to 40 mesh or less, 2 L of distilled water was added to 100 g of the powder, and extracted at 100 ° C. for 3 hours. Then, it centrifuged (8500 rpm, 10 minutes), the supernatant was filtered, and the extract and the residue were isolate | separated. The residue was concentrated under reduced pressure, and then freeze-dried to obtain 13.4 g of the antioxidant composition B of the present invention.

(Antioxidant capacity measurement method-DPPH)
The measurement of free radical scavenging activity was carried out by a method using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) having a relatively stable radical. In this method, DPPH having a maximum absorption at 517 nm in a radical state is reduced by an antioxidant substance, and a decrease in absorbance at 517 nm is captured to evaluate the antioxidant ability.

(Test Example 1) DPPH measurement DPPH was measured using a spectrophotometer.
Antioxidant composition A obtained in Example 1 was weighed and placed in an Eppendorf tube, and distilled water was added so that the concentration of antioxidant composition A was 10 mg / mL. The sample solution was serially diluted to prepare sample solutions of 18.75, 37.5, 150, and 300 μg / mL. 50 μL each of this solution was transferred to another test tube, and 450 μL of 50 mM Tris-HCl and 1 mL of 50 mM DPPH were added thereto. These sample solutions were measured with a spectrophotometer (517 nm) to measure DPPH radical scavenger activity. As a comparative control, an extract obtained by extracting green tea in the same manner as in Example 1 was used.
The results are shown in Table 1.

From the above results, it was revealed that the antioxidant composition A of the present invention has DPPH inhibitory activity equivalent to that of the green tea extract, and the IC ₅₀ is not much different.

(Active oxygen scavenging activity measurement method-SOD measurement)
Superoxide dismutase (Superoxide Dismutase, SOD) superoxide anion (· _{O 2} ^-, active oxygen) with an enzyme that from the oxygen and hydrogen molecules by removing electrons, are often localized to the cell nucleus. It plays a role in reducing oxidative stress.
The active oxygen scavenging activity was measured by the scavenging ability for superoxide generated by the xanthine oxidase / hypoxanthine system. In this measurement method, a chemiluminescence reagent with high specificity to superoxide, when 2-methyl-p-methoxyphenylethynylimidazopyrazinone (MPEC), a luciferin derivative, was used and added to the xanthine oxidase / hypoxanthine system The chemiluminescence of was measured with a luminometer.

(Test Example 2) Measurement of SOD-like activity Antioxidant composition B obtained in Example 2 was prepared to 18.75, 37.5, 75, 150, and 300 μg / mL, and each 10 μL and 300 μM MPEC luminescence reagent. 10 μL, xanthine oxidase 60 μL, KH ₂ PO ₄ / NaOH buffer 170 μL and hypoxanthine 50 μL were prepared. This solution was measured with a Veritas microplate luminometer to detect superoxide. The activity was determined from the following formula.
SOD-like activity% = 1− (reacted sample / control) × 100
The results are shown in Table 2.

  From the above results, it was revealed that the antioxidant composition B has a high SOD-like activity.

(Example 3) Preparation of antioxidant composition 3
The dried fruit of onamomi was pulverized to 40 mesh or less, 3 L of distilled water was added to 150 g of the powder, and extracted at 100 ° C. for 3 hours. Then, it centrifuged (8500 rpm, 10 minutes), the supernatant was filtered, and the extract and the residue were isolate | separated. The residue was concentrated under reduced pressure to 400 mL. Ethanol was added to this concentrated solution to prepare 500 mL (final ethanol concentration 20%), and then allowed to stand at room temperature for 24 hours to precipitate insoluble components. The precipitate was separated by centrifugation (8500 rpm, 10 minutes), the supernatant was concentrated under reduced pressure, and 1 L of distilled water was added and redissolved. Then, the insoluble component was removed by filtration, and the filtrate was concentrated under reduced pressure and then freeze-concentrated and dried to obtain 11.4 g (yield: 7.6%) of the antioxidant composition C of the present invention. Further, ethanol was added to the concentrate obtained in the same manner to precipitate insoluble components when the final ethanol concentration was 80%, and the same operation was performed to obtain 9.8 g of the antioxidant composition D of the present invention.

(Test Example 3) DPPH Measurement Antioxidant composition D obtained in Example 3 was weighed and placed in an Eppendorf tube, and distilled water was added so that the concentration of antioxidant composition D was 10 mg / mL. The sample solution was diluted to prepare a 1000 μg / mL sample solution. DPPH radical scavenger activity was measured in the same manner as in Test Example 1. For comparison, an extract obtained by extracting green tea in the same manner as in Example 3 was used.
The results are shown in Table 3.

  From the above results, the antioxidant composition D was found to have high DPPH inhibitory activity.

(Test Example 4) Measurement of SOD-like activity Antioxidant composition D obtained in Example 3 was prepared to 31, 62.5, 125, 250, 500, 1000 μg / mL, and SOD was prepared in the same manner as in Test Example 2. Like activity was determined.
The results are shown in Table 4.

  From the above results, the SOD-like activity higher than that of the untreated ethanol (antioxidant composition A) was observed in the antioxidant composition D.

(Example 4) Preparation of antioxidant composition-containing food (tablet confectionery) Antioxidant composition A 5 g obtained in Example 1, lactose 30 g, DHA-containing powdered oil (Suncoat DY-5; manufactured by Taiyo Kagaku) 12 g , 4 g of sucrose fatty acid ester and 4 g of yogurt flavor were mixed, and the mixture was pressure-molded using a rotary tableting machine to obtain 300 mg of the antioxidant composition-containing food and beverage product (tablet cake) of the present invention. Obtained.

(Example 5) Preparation of antioxidant composition-containing beverage Antioxidant composition B 5 g obtained in Example 2, 1/5 concentrated grapefruit clear fruit juice 2.1 g, erythritol 30 g, citric acid crystals 2.5 g, citric acid Trisodium 0.5g, L-ascorbic acid 0.5g, calcium lactate 1.93g, CCP 0.15g, grapefruit flavor 1.0 are mixed and dissolved in water to make a total volume of 1000ml, and it is filled into a 100ml bottle. After sealing with a cap, it was sterilized by heating at 90 ° C. for 30 minutes to obtain an antioxidant composition-containing food or drink according to the present invention.

(Example 6) Preparation of antioxidant composition-containing beverage (vegetable juice mixed drink) Antioxidant composition B 0.2 g obtained in Example 2 and guar gum degradation product (Sunfiber R; manufactured by Taiyo Kagaku Co., Ltd.) 3 g The antioxidant composition containing food / beverage product (vegetable juice mixed drink) of this invention was obtained by adding and mixing and dissolving in 100 ml of commercially available vegetable juice mixed drink.

Example 7 Preparation of Antioxidant Composition-Containing Cookies After putting 4 g of the antioxidant composition B obtained in Example 2 and 200 g of commercially available cake mix powder into a container, put 35 g of butter and mix with a wooden eggplant. It was. 25g of beaten egg was added to it and kneaded well until it became a smooth dough. The dough is taken out on a table on which the flour has been shaken, and the flour is further shaken and stretched to a thickness of 5 mm with a rolling pin, extracted with a round shape, and baked in an oven at 170 ° C. for 10 minutes, and about 5 g of this invention. An antioxidant composition-containing cookie was obtained.

Example 8 Preparation of Antioxidant Composition-Containing Yogurt In Example 1, antioxidant composition A 1 g, commercially available skim milk (manufactured by Meiji Dairies Co., Ltd., 95 g protein content), and commercially available unsalted butter (Snow Brand Milk Products Co., Ltd.) 35 g) was dissolved in 0.8 L of warm water, homogenized, and the total amount was adjusted to 1 L. Next, the mixture is sterilized by heating at 90 ° C. for 15 minutes, cooled, inoculated with 3 g of commercially available lactic acid bacteria starter (manufactured by Hansen) (2 g of Streptococcus thermophilus and 1 g of Lactobacillus bulgaricus), mixed uniformly, and divided into a 100 ml container. Note, filled, sealed, fermented at 37 ° C. for 20 hours and then cooled to obtain the antioxidant composition-containing yogurt of the present invention.

(Example 9) Preparation of oral liquid food containing antioxidant composition 50 g sodium caseinate (manufactured by DMV), 42.5 g egg white enzyme degradation product (manufactured by Taiyo Kagaku Co., Ltd.), 100 g dextrin (manufactured by Matsutani Chemical Co., Ltd.) The aqueous phase was prepared in a tank. Separately, 45 g of MCT (manufactured by Kao Corporation), 17.5 g of palm oil (manufactured by Fuji Oil Co., Ltd.), 35 g of safflower oil (manufactured by Taiyo Oil & Fats Co., Ltd.), 0.7 g of lecithin (manufactured by Taiyo Kagaku Co., Ltd.), defoaming 1 g of an agent (manufactured by Taiyo Chemical Co., Ltd.) was mixed and dissolved to prepare an oil phase. The oil phase was added to the aqueous phase in the tank, stirred and mixed, then heated to 70 ° C., and further homogenized with a homogenizer at a pressure of 14.7 MPa. Next, the mixture was sterilized at 90 ° C. for 10 minutes, then concentrated and spray-dried to prepare about 260 g of an intermediate product powder. 200 g of this intermediate product powder, 4 g of the antioxidant composition A obtained in Example 1, 156 g of dextrin (manufactured by Matsutani Chemical Co., Ltd.), 18 g of guar gum degradation product (Sunfiber R; manufactured by Taiyo Chemical Co., Ltd.), a small amount of vitamins and minerals and powder A fragrance was added and mixed uniformly to obtain about 380 g of an oral liquid food containing an antioxidant composition.

(Example 10) Preparation of antioxidant composition-containing tablet 10 g of the antioxidant composition A obtained in Example 1, 5 g of crystalline cellulose, 13.8 g of corn starch, 32.5 g of lactose, and 3.3 g of hydroxypropylcellulose were mixed. Granulated. Add 1.0 g of magnesium stearate to the granulated product, mix uniformly, and press-mold the mixture using a rotary tableting machine to obtain a tablet containing the antioxidant composition of the present invention, each containing 130 mg. It was.

The embodiment of the present invention and the target product are as follows.
(1) An antioxidant composition characterized by containing fruit, seeds or extracts thereof of Onamomi.
(2) The antibacterial fruit or seed extract is extracted from the fruit, fruit juice or seed of onamomi with any one of water, base, acid, and hydrophilic solvent. Oxidizing composition.
(3) The antioxidant composition according to (1) or (2) above, wherein the extract of fruit and seeds of onamomi is extracted from the fruit, fruit juice or seeds of onamomi with water.
(4) The antioxidant composition according to (2), wherein the hydrophilic solvent is one or more lower alcohols selected from the group consisting of methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, and butyl alcohol. object.
(5) The antioxidant composition characterized by being fractionated by any one or more of organic solvents from the fruit and seed extract of the ona fir of any one of (1) to (4) above .
(6) The antioxidant composition according to (5) above, wherein the organic solvent is ethyl alcohol.
(7) The antioxidant composition according to the above (6), wherein the extract of fruit and seeds of onamomi is fractionated as a soluble fraction with ethyl alcohol.
(8) The ethanol concentration when fractionating fruit, seed or extract thereof of ethanol with ethanol is 20 to 80%, and the ethanol-soluble fraction as described in (7) above Antioxidant composition.
(9) The antioxidant composition according to any one of (1) to (8) above, wherein the extract of fruit and seeds of onamomi enhances the effect by chromatography or a column using a hydrophobic resin.
(10) The antioxidant composition as described in (11) above, wherein the hydrophobic resin is based on phenol, styrene, acrylic, epoxyamine, pyridine, methacryl, or the like.
(11) Food / beverage products containing the antioxidant composition in any one of said (1)-(10).
(12) A quasi-drug containing the antioxidant composition according to any one of (1) to (10).
(13) A pharmaceutical comprising the antioxidant composition according to any one of (1) to (10).
(14) A feed comprising the antioxidant composition according to any one of (1) to (10).

  The antioxidant composition containing the fruit, seed or extract thereof of the onam fir obtained in the present invention has a high DPPH free radical scavenging ability and SOD-like activity value. Used for food and drink, quasi-drugs and medicines, aging, stroke, autoimmune disease, cancer, cataract, fatty liver, myocardial infarction, ischemic heart disease, Alzheimer's syndrome, diabetes, diabetic nephropathy, arteriosclerosis It is possible to suppress the progression of various diseases such as allergies and hay fever and abnormal pigmentation such as spots and freckles and the deterioration of food.

Claims (7)

An antioxidant composition comprising onaomi fruit, seeds or an extract thereof. 2. The antioxidant composition according to claim 1, wherein the extract of fruit or seeds of onamomi is extracted with at least one selected from the group consisting of water, base, acid, hydrophilic solvent, and acetone. 3. An antioxidant composition, wherein the extract of fruit or seed of onamomi according to claim 1 or 2 is fractionated with an organic solvent. The antioxidant composition according to claim 3, wherein the organic solvent is at least one selected from the group consisting of ethyl alcohol and acetone. Food / beverage products containing the antioxidant composition in any one of Claims 1-4. A quasi-drug containing the antioxidant composition according to any one of claims 1 to 4. A pharmaceutical comprising the antioxidant composition according to claim 1.