KR102176394B1 - A composition for immune enhancement comprising taheebo extract - Google Patents

Abstract

The present invention relates to a composition for enhancing immunity, comprising a taheebo extract and Illicium verum as an active ingredient.

Description

A composition for enhancing immunity containing tahibo extract as an active ingredient {A COMPOSITION FOR IMMUNE ENHANCEMENT COMPRISING TAHEEBO EXTRACT}

The present invention relates to a composition for enhancing immunity comprising a tahibo extract as an active ingredient, and can be used for various purposes such as pharmaceuticals and foods.

All living organisms constantly need a lot of nutrients in order to live life, and nutrients are always supplied from the outside in the form of food or some are synthesized in the body.

Food contains substances necessary to maintain human life phenomena, and humans can sustain life by ingesting food, digesting it in the digestive system, absorbing it, and supplying it to tissue cells in the body.

Recently, as the standard of living has increased and the diet has become westernized, the incidence of adult diseases such as obesity, diabetes, high blood pressure and heart disease due to overnutrition and lack of exercise is increasing. In addition, as the population gradually ages due to the extended life expectancy, interest in health is gradually increasing.

Health promotion is aimed at increasing resistance to diseases, especially chronic degenerative diseases or infections, and continuing stress, and increasing daily activity. To this end, improving nutrition, strengthening immune function, proper exercise and rest, and mental activity. Management that affects the overall lifestyle such as continuity is required.

Immune is a self-defense system that exists in the body and is a process by which the human body recognizes, removes, and metabolizes various substances or living organisms that invade from the outside as foreign substances to itself.

The living body acts as a defense against various external factors (pathogenic microorganisms, etc.), which is called the immune system. The body's immune system is a body defense mechanism against infection, and is composed of two mechanisms: innate immunity that has been born since birth and acquired immunity that is obtained through adaptation to life.

Acquired immunity is composed of immune cells of B lymphocytes and T lymphocytes, acting specifically against foreign antigens, exhibiting an immune response, and is activated through immune memory.

Innate immunity is also called natural immunity, and unlike acquired immunity, it means a non-specific immune response to an antigen.

Innate immunity is the fastest response of a host that is induced and operates within a few hours after infection. Receptors in the innate immune system recognize a wide range of viral infections and humoral factors such as cytokines and chemokines. factor).

The key innate immune response to viral infection is that the expression of interferon is induced, and interferon, which is considered the most important cytokine in viral infection, is known to have excellent antiviral activity.

Therefore, the enhancement of immune function can be very closely related to the proliferation or infection of viruses in the human body as well as the regulation of immune function in the human body.

Factors affecting immune function include genetic and environmental factors, age, sex, psychological stress, nutritional status, dietary habits, and disease.

Health functional foods with the function of regulating immune function are classified into the function of enhancing the reduced immune function, the function of controlling the excessive immune function, and the function of regulating the autoimmune function that regulates the immune response to magnetic components.

When the immune function is lowered, hypersensitivity reactions may occur.These hypersensitivity reactions vary, such as asthma, seasonal or homogenous rhinitis, allergic sinusitis, conjunctivitis, food and drug allergies, atopic dermatitis, urticaria, allergies caused by bee stings, etc. Appears to be.

In order to overcome immune function disorders due to various causes, various immunity enhancers are used, but there is a problem of side effects and biosafety, so long-term use is possible and an immunity enhancing composition suitable for prevention is required.

Under these circumstances, many efforts have been made to overcome the shortcomings of existing antiviral agents in both overseas and domestic markets, and as part of that effort, studies on antiviral efficacy have been actively conducted on herbal extracts and plant extracts in Korea. It is going on.

The present invention is to solve the problems of the prior art described above, an object of the present invention is to provide a functional food drug derived from a natural plant excellent in biosafety.

According to one aspect of the present invention, there is provided a composition for enhancing immunity comprising tahibo extract and octagonal fennel as an active ingredient.

In one embodiment, the composition may further include a flower mushroom extract.

In one embodiment, the composition may further include fucoidan or sasamin extract.

According to another aspect of the present invention, tahibo extract and octagonal fennel; And there is provided a health functional food for enhancing immunity comprising at least one selected from the group consisting of a flower mushroom extract, fucoidan, and sasam extract.

In one embodiment, the health functional food may further include antiviral use.

In one embodiment, the virus may be one or more selected from the group consisting of coronavirus, influenza virus, swine influenza A virus (Influenza A virus subtype H1N1), and adenovirus. .

According to another aspect of the present invention, tahibo extract and octagonal fennel; And there is provided a pharmaceutical composition for improving immunity comprising at least one selected from the group consisting of a flower mushroom extract, fucoidan, and sasam extract.

In one embodiment, the pharmaceutical composition may further include a use for preventing and treating viral diseases.

The composition according to an aspect of the present invention includes a natural extract as an active ingredient, and thus has excellent biosafety as well as excellent immunity-promoting activity, and thus can be usefully utilized not only as a health functional food but also for the treatment of related diseases.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Terms used in the present specification have selected general terms that are currently widely used as possible while considering functions in the present invention, but this may vary depending on the intention or precedent of a technician working in the field, the emergence of new technologies, and the like.

In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning of the terms will be described in detail in the description of the corresponding invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall contents of the present invention, not a simple name of the term.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in this application. Does not.

The numerical range includes the numerical values defined in the above range. All maximum numerical limits given throughout this specification include all lower numerical limits as if the lower numerical limits were expressly written. All minimum numerical limits given throughout this specification are inclusive of all higher numerical limits as if the higher numerical limits were expressly written. All numerical limits given throughout this specification will include all better numerical ranges within the wider numerical range, as if the narrower numerical limits were expressly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

One aspect of the present invention provides a composition for enhancing immunity comprising tahibo extract and octagonal fennel as an active ingredient.

The “immunity” refers to a mechanism that protects the organism from diseases by detecting pathogens and tumor cells in an organism, and disabling it. The “immunostimulation” refers to various diseases such as cancer and inflammation. A set of therapeutic or prophylactic strategies to augment the body's defense mechanisms against the disease can be included.

The “Taheebo” is made from the Tabebuiaavellanedae tree of the Bignoniaceae family, which grows naturally in some areas of the Amazon.

The taheebo can only be used as a raw material with an inner bark of about 7 mm between the outer skin of the Tabevia Abellanedae tree and the wood. Among the ingredients of tahibo, the anticancer effect of quinones against naphtoquinon (NFD) is attracting attention, and it is known that NFD plays a role of inhibiting tumor invasion by inhibiting the formation of new blood vessels.

The " Ilicium verum Hooker fil. " refers to the fruits of magnolias and evergreen trees, and is generally called star anise because eight pods wrapped in a hard shell are attached like stars.

The octagonal fennel is widely used as a spice after drying in Paju, making it into a powder form, and anti-cancer (Journal of Medicinal Plants Research, 4(24): 2666-2673. 2016), antioxidant (Food Chemistry, 104(1): 59- 66, 2007), and anti-inflammatory activity (Journal of Ethnopharmacology, 144(1): 182-189, 2012) have been reported.

The “contained as an active ingredient” means containing an amount sufficient to provide a therapeutic or prophylactic effect to the individual to be administered, and the natural extract-based composition is excellent in biosafety, so those skilled in the art will have a quantitative upper limit Can be adjusted appropriately.

The "extract" refers to a solvent in which the active ingredient contained in the raw material for extraction is transferred by contacting the solvent with the raw material for extraction under specific conditions. You can include all regardless.

For example, the extract may include extracting a component soluble in a solvent from a natural product using water or an organic solvent, and extracting only a specific component of a natural product, such as an oil.

The extract may be obtained by drying and powdering the raw material for extraction, or by various conventionally known extraction methods such as hot water extraction and ethanol extraction.

The extract may be extracted with water, alcohol, ethyl acetate, acetone, nucleic acid, dichloromethane, or a mixed solvent thereof, and preferably with ethanol at a concentration of 60 to 90% (w/w).

Since the extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent, it may be changed in consideration of the selectivity of the physiologically active substance according to the solvent.

The extract is extracted by conventional methods such as reflux extraction, pressure extraction, ultrasonic extraction, etc. for about 1 to 24 hours with a solvent having a volume of 8 to 12 times the weight of the raw material by washing the extraction raw material with water, drying and grinding, and It can be prepared by filtration.

The extract can be obtained in powder form by an additional process such as distillation under reduced pressure or freeze drying.

The extract may also include an extract that has undergone a conventional purification process. For example, the extract may be subjected to various purification methods additionally performed, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (one prepared for separation according to size, charge, hydrophobicity or affinity). It may contain a fraction obtained through.

The composition may further include a flower mushroom extract.

The " Sparassis crispa " is an edible mushroom belonging to the flower family of the Minorum Mushroom Order, and is rich in dietary fiber, and in particular, the side chain of beta-1,6-linked to the main chain of which glucose is linked by a beta-1,3-bond It is known that the content of beta-glucan (β) is 10 times higher than that of edible mushrooms such as Ganoderma lucidum or Pseudomonas mushrooms, and has various physiological activities such as anti-tumor and anti-diabetes.

The composition may further include fucoidan or sasamin extract.

The “Fucoidan” is a polysaccharide containing a sulfuric acid group as a main component of fucose present in seaweed. It is generally present in a large amount in brown algae such as kelp, seaweed, and soybean. These fucoidan improve blood circulation, anticancer, etc. Not only is it excellent in physiological activity, but it is also known to be effective in whitening, moisturizing, and wrinkle improvement.

The “sasam” refers to the root of the plantain family ( Adenophora triphylla var. japonica Hara ) or the dangzandae. Sasamin is used as a medicinal herb, and supplements essence to moisten the lungs, cools heat, and removes phlegm to dry the throat, dry cough, phlegm, seawater, and asthma.

The present inventors have found out that the natural extract can be implemented with excellent immune enhancing activity through the interaction.

Another aspect of the present invention is tahibo extract and octagonal fennel; And it provides a health functional food for improving immunity comprising at least one selected from the group consisting of a flower mushroom extract, fucoidan, and sasam extract.

The composition for enhancing immunity may be used as a food composition, such as an immunity enhancing or antiviral-related health functional food.

The health functional food refers to a food manufactured and processed using raw materials or ingredients that have useful functionality for the human body according to the Health Functional Food Act, and the functionality is to control nutrients for the structure and function of the human body or It may mean ingestion for the purpose of obtaining a useful effect for health purposes such as action.

The health functional food may contain ordinary food additives, and the food additives comply with the standards and standards for the relevant item in accordance with the general rules and general test methods of food additives approved by the Ministry of Food and Drug Safety, unless otherwise specified. The suitability can be determined by this.

The items described in the food additive code include, for example, ketones, glycine, potassium citrate, nicotinic acid, cinnamic acid, etc., chemical compounds such as dark pigments, licorice extract, crystalline cellulose, high color pigments, natural additives such as guar gum, sodium L-glutamate preparations, noodles Mixed preparations, such as an added alkali agent, a preservative preparation, and a tar color preparation, are mentioned.

The composition for improving immunity may be variously used in foods and beverages for improving health, and for example, various foods, beverages, gums, teas, vitamin complexes, health functional supplements, food additives, and the like.

The health functional food may be manufactured and processed into any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills for the purpose of improving health.

Specifically, for the health functional food in the form of a tablet, a mixture of an excipient, a binder, a disintegrant, and other additives together with an active ingredient is granulated in a conventional manner, and then compression molded by putting a lubricant or the like, or directly compression molding the mixture. It can be manufactured.

In addition, the health functional food in the form of a tablet may contain a mating agent or the like, if necessary, and may be coated with a suitable coating agent if necessary.

Among the capsule-type health functional foods, hard capsules can be prepared by filling a conventional hard capsule with a mixture of additives such as excipients, granular or skinned granules thereof, and additives such as active ingredients and excipients. A mixture of and can be prepared by filling a capsule base such as gelatin.

The soft capsules may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, and the like, if necessary.

The ring-shaped health functional food can be prepared by molding a mixture of excipients, binders, disintegrants, etc. together with the active ingredients in an appropriate way, and if necessary, the skin is coated with a white sugar or other suitable skin-forming agent, or starch, talc, or a suitable material. It can also make you happy.

The granular health functional food may be prepared in a granular form by an appropriate method of a mixture of an excipient, a binder, and a disintegrant together with an active ingredient, and may contain a flavoring agent, a flavoring agent, and the like, if necessary.

In addition, the definitions of terms for the excipients, binders, disintegrants, lubricants, flavoring agents, flavoring agents, and the like are described in documents known in the art, and may include those having the same or similar functions.

The health functional food may further include antiviral use.

The “anti-virus” is used in conjunction with an antiviral effect or action, and is used to inhibit viral infection and proliferation, a filter pathogen that causes various diseases by parasitic and proliferation in living cells such as plants, animals, and bacteria. Means that.

Specifically, the antivirus inhibits the proliferation of at least one virus selected from the group consisting of coronavirus, influenza virus, swine influenza A virus (Influenza A virus subtype H1N1), and adenovirus. It may mean the activity of killing or killing, but it is sufficient if it can kill or inhibit the proliferation of various viruses in addition to that.

Another aspect of the present invention is tahibo extract and octagonal fennel; And it provides a pharmaceutical composition for enhancing immunity comprising at least one selected from the group consisting of a flower mushroom extract, fucoidan, and sasam extract.

The composition can be usefully used in the prevention, improvement or treatment of diseases caused by immunodeficiency, immunodeficiency or damage to the immune system by enhancing immune activity.

The "prevention" means any action that reduces the frequency or severity of pathological symptoms. Prevention can be complete or partial. In this case, it may refer to a phenomenon in which symptoms caused by inflammation in the individual decrease compared to the case where the composition is not used.

The “treatment” refers to any action that clinically intervenes in order to change the natural process of a target or cell to be treated, and may be performed while a clinical pathology is in progress or to prevent it.

The desired therapeutic effect is to prevent the occurrence or recurrence of the disease, alleviate symptoms, reduce all direct or indirect pathological consequences of the disease, prevent metastasis, reduce the rate of disease progression, or It may include alleviating or temporarily alleviating the condition, or improving the prognosis.

In addition, the pharmaceutical composition may further include a use for preventing and treating viral diseases.

The “viral disease” is a disease caused by infection with a virus, and the pharmaceutical composition includes a coronavirus, an influenza virus, a swine influenza A virus (Influenza A virus subtype H1N1), and an adenovirus ( adenovirus) can effectively relieve symptoms caused by infection.

The composition may be administered in the form of oral delivery or parenteral delivery. The composition may be administered systemically or locally, and the administration may include oral administration and parenteral administration. The composition may be formulated with an appropriate amount of a pharmaceutically acceptable vehicle or carrier to provide an appropriate dosage form.

The composition may further include a carrier, an excipient, and a diluent used in the preparation of a pharmaceutical composition. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil may be mentioned, but the present invention is not limited thereto.

In addition, the composition may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, external preparations, suppositories, and sterile injectable solutions.

Solid preparations for oral administration may include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may contain at least one excipient, such as starch, calcium carbonate, sucrose, or the like, in the natural extract and its fractions. It can be prepared by mixing lactose or gelatin. In addition, lubricants such as magnesium stearate and talc may be used in addition to the excipients.

Liquid preparations for oral administration may include suspensions, liquid solutions, emulsions, syrups, etc., and various excipients, such as wetting agents, sweeteners, fragrances, preservatives, etc., may be used in addition to simple diluents such as water and liquid paraffin.

Formulations for parenteral administration may be sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories.

The non-aqueous solvent and suspension may be propylene glycol, polyethylene glycol, vegetable oil such as olive oil, or injectable ester such as ethyl oleate. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin paper, and glycerogelatin may be used.

The pharmaceutical composition may be administered to a subject in a pharmaceutically effective amount. The “pharmaceutically effective amount” refers to an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, drug Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including co-used drugs and other factors well known in the medical field.

The pharmaceutical composition may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is preferable to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and this can be easily determined by a person skilled in the art.

It is apparent that the present invention is further elaborated through the following examples, but the present invention is not limited by the following examples.

Examples and Comparative Examples

In order to verify the physiological activity of the obtained extract, samples were mixed as shown in Tables 1 and 2 to set Examples and Comparative Examples.

Each raw material was obtained and used on the market.

[Content (parts by weight)] division Example One 2 3 4 5 6 Taheebo extract 200 200 200 200 200 200 Octagonal fennel 150 75 75 75 50 37.5 Blossom Mushroom Extract - 75 - - 50 37.5 Fucoidan - - 75 - 50 37.5 Four ginseng extract - - - 75 - 37.5

[Content (parts by weight)] division Comparative example One 2 3 4 5 6 7 8 9 Taheebo extract 350 - - - - 200 200 200 - Octagonal fennel - 350 - - - - - - 87.5 Blossom Mushroom Extract - - 350 - - 150 - - 87.5 Fucoidan - - - 350 - 150 - 87.5 Four ginseng extract - - - - 350 - - 150 87.5

Experimental Example 1: Cell culture and toxicity evaluation (MTT assay)

The cell line RAW264.7 macrophage cell was purchased and used from the American Type Culture Collection (ATCC, Manassas. USA).

DMEM (Dulbecco's modified of Eagle's medium, Hyclone, USA) medium containing 10% Fetal Bovin Serum (FBS, Hyclone USA), 100 units/mL penicillin/Streptoycin (Hyclone, USA) was used for cell culture, 37 C. Incubated in an incubator (Thermo, Forma, Germany) in the presence of 5% CO ₂ .

Cell viability was measured by measuring the concentration of the generated formazan with a spectrophotometer using the change from Tetrazolium salt (WST-1) to formazan by mitochondrial dehydrogenase in living cells.

MTT assay was performed according to the protocol using Ez-cytox (Dozen, Korea).

Raw264.7 cells were dispensed into 96 wells at 5x10 ³ cells/100μL and cultured for 24 hours in a 37°C, 5% CO ₂ incubator.

The samples were treated by concentration and incubated for 24 hours.

After incubation, it was replaced with a medium containing 10% MTT reagent, reacted at 37° C. for 1 hour, and then measured with a microplate reader (Epoch, Biotek) at 450 nm wavelength.

Toxicity evaluation based on cell viability for each sample was measured by repeating three times, and the mean value and standard deviation were calculated.

As a result of the measurement, the samples of Examples and Comparative Examples did not show significant cytotoxicity at all concentrations (10, 100, 1000 μg/mL).

Experimental Example 2: Evaluation of immune efficacy (NO assay)

To measure the concentration of Nitric Oxide (NO), the absorbance was measured at 540 nm using a grease reaction.

N-(1-naphthyl)-ethylenediamine, sulfanilamide, and NO ₂ ^- react to form azo couling, and the two ring shapes have maximum absorbance values at a wavelength of 540 nm.

Raw264.7 cells were dispensed in a 24 well plate at 5x10 ⁴ cells / 250 μL and incubated in a 37°C, 5% CO ₂ incubator for 24 hours.

The sample was treated at a concentration of 500 μg/mL and incubated in a CO ₂ incubator for 48 hours. Take 50 μL of the cell culture supernatant, mix 50 μL of N1 buffer, and react at room temperature for 5 to 10 minutes.

After 50 μL of N2 buffer was mixed and reacted for 10 minutes, absorbance at 560 nm was measured using a microplate reader (Epoch, Biotek).

It was measured three times in the same way, and the average value and standard deviation thereof were calculated.

The measured value was expressed as the increase rate of NO concentration compared to the control without the sample treatment.

Remark NO generation ability (%) Example 1 236 Example 2 274 Example 3 269 Example 4 285 Example 5 337 Example 6 393 Comparative Example 1 136 Comparative Example 2 121 Comparative Example 3 118 Comparative Example 4 125 Comparative Example 5 132 Comparative Example 6 145 Comparative Example 7 138 Comparative Example 8 148 Comparative Example 9 143

Referring to Table 3, the sample of the above example significantly improved the NO generation ability compared to the sample of the comparative example. The results show that in the case of the example in which Tahibo extract and octagonal fennel were applied at the same time, the immunity enhancing effect would be significantly increased due to the synergistic effect of each raw material Suggests that you can.

Experimental Example 4: Evaluation of immune efficacy (evaluation of TNF-a production amount)

In order to examine the immunity increasing efficacy, Raw264.7 cells were dispensed in a 24 well plate at 5×10 ⁴ cells/250 μL and incubated for 24 hours in a 37°C, 5% CO ₂ incubator.

The sample was treated at a concentration of 500 μg/mL, incubated in a CO ₂ incubator for 4 hours, and then the cell suspension was centrifuged to precipitate the cells and the supernatant was collected.

The amount of TNF-a produced in the supernatant was quantified according to the method described in the user's manual using an ELISA kit (R&D systems Inc., Minneapolis, MN, USA) (Table 4).

Remark TNF-a production amount (pg/mL) Example 1 5231 Example 2 5858 Example 3 5721 Example 4 6026 Example 5 6519 Example 6 7262 Comparative Example 1 2356 Comparative Example 2 2125 Comparative Example 3 2231 Comparative Example 4 1953 Comparative Example 5 2205 Comparative Example 6 2834 Comparative Example 7 2753 Comparative Example 8 2634 Comparative Example 9 2731

Referring to Table 4, the sample of the above example significantly improved the TNF-a production ability compared to the sample of the comparative example.

The above results suggest that in the case of the examples in which the tahibo extract and the octagonal fennel are applied at the same time, the immunity enhancing activity can be remarkably increased due to the synergistic effect of each raw material.

Experimental Example 5: Evaluation of antiviral activity

Samples of Examples and Comparative Examples were subjected to the sulforhodamine B (SRB) method to confirm the cytopathic effect (CPE) inhibitory activity caused by the virus.

Specifically, in a 96-well culture plate (BD Biosciences, San Jose, CA, USA) 2 × 10 ⁴ vero cells per well (American Type Culture Collection; ATCC) fetal bovine serum (fetal bovine) Serum; FBS) and a minimum essential medium (MEM) containing a 1% antibiotic-antifungal solution (Gibco, Germany) was inoculated and incubated at 37°C for 24 hours.

After 24 hours, the medium of each well was removed, washed once with phosphate buffered saline (PBS) solution, and replaced with a medium containing virus (Korea Centers for Disease Control and Prevention, KCDC) to convert Vero cells to virus. Each was infected.

Samples of Examples and Comparative Examples were treated with the infected cells at concentrations of 1, 10, and 100 μM to each of the cells and cultured for 48 hours.

Each well was treated with SRB (Sigma-Aldrich, USA), and absorbance was measured at a wavelength of 540 nm using a VERSAmax microplate reader (Molecular Devices, Palo Alto, CA, USA), and the degree of viability of cells was confirmed. The baseline absorbance was set to 620 nm.

Measurement values of all experimental groups were normalized to the degree of cell viability in non-infected normal control cells, and then the relative cell viability (%) of the infected cells was calculated according to the maximum viability of the non-infected cells.

In order to confirm cytotoxicity, non-infected Vero cells were treated with the above compounds to evaluate cell viability.

In all experiments, the same process was repeated three times, the average value was calculated, and the viability of virus-infected cells at a concentration of 10 μM is shown in Table 5.

Remark Infected cell viability (%) Coronavirus Influenza virus Example 1 69.3 73.6 Example 2 73.7 78.8 Example 3 75.2 80.6 Example 4 73.5 78.6 Example 5 79.2 85.4 Example 6 87.2 90.2 Comparative Example 1 38.2 40.2 Comparative Example 2 34.6 33.9 Comparative Example 3 36.8 34.6 Comparative Example 4 40.1 38.5 Comparative Example 5 34.7 32.0 Comparative Example 6 41.2 39.8 Comparative Example 7 42.8 43.8 Comparative Example 8 39.2 37.4

Referring to Table 5, when the sample of Example was treated with virus-infected Vero cells, the cell survival rate increased as the treatment concentration increased, and the sample of Example significantly increased the cell survival rate compared to the sample of Comparative Example or the untreated group. Made it.

Experimental Example 6: Evaluation of antioxidant activity

The extracts of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging acticity.

The DPPH measurement method is a method of measuring the absorbance at 540n to determine the degree of decolorization by scavenging the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical) by an inhibitor.

Vitamin C was used as a control, and the same experiment was repeated 3 times for the accuracy of the experiment, and the results are shown in Table 6 below.

[Concentration to eliminate 50% of radical DPPH] division SC ₅₀ (μg/mL) Example 1 139.2 Example 2 127.8 Example 3 125.0 Example 4 124.5 Example 5 120.8 Example 6 118.8 Comparative Example 1 214.3 Comparative Example 2 209.9 Comparative Example 3 197.2 Comparative Example 4 196.0 Comparative Example 5 192.9 Comparative Example 6 185.4 Comparative Example 7 176.0 Comparative Example 8 182.9 Comparative Example 9 175.4 Control (vitamin C) 121.5

The lower the value of SC _50, the higher the scavenging activity of DPPH free radicals is. Referring to Table 6, the sample of Example showed remarkably superior antioxidant effect compared to the comparative example.

In particular, the sample of the Example containing both the Tahibo extract and the octagonal fennel at the same time showed remarkably superior free radical scavenging activity compared to the comparative sample containing only part of the sample, and the result suggests a synergistic effect according to the combination of each composition.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains will be able to understand that other specific forms can be easily modified without changing the technical spirit or essential features of the present invention will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be construed as being included in the scope of the present invention.

Claims (8)

Health functional food for enhancing immunity containing taheebo extract and octagonal fennel as active ingredients. The method of claim 1,
A health functional food that further contains a flower mushroom extract. The method of claim 2,
Health functional food further comprising fucoidan or sasamin extract. Tahibo extract and octagonal fennel; And
A health functional food for antiviral against coronavirus or influenza virus comprising at least one selected from the group consisting of blossom mushroom extract, fucoidan, and sasam extract. The method according to any one of claims 1 to 3,
Health functional food that further includes antiviral use for coronavirus or influenza virus. delete A pharmaceutical composition for the prevention or treatment of diseases caused by coronavirus or influenza virus, comprising taheebo extract and octagonal fennel as active ingredients. The method of claim 7,
A pharmaceutical composition further comprising at least one selected from the group consisting of flower mushroom extract, fucoidan, and sasam extract.