KR20180042541A - Cosmetic composition comprising the very high-pressure extract of narcissus tazetta bulb fermentation - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented ultra-high pressure extract of Narcissus tazetta bulbs obtained by fermenting Narcissus tazetta bulbs and then treating the same with ultra-high pressure, and extracting the same. More specifically, the present invention relates to a cosmetic composition comprising a fermented ultra-high pressure extract of Narcissus tazetta bulbs having obtained flavonoids in a high yield among active components by fermenting Narcissus tazetta bulbs with one or more selected from the group consisting of yeast, lactic acid bacteria, bacteria of Bacillus sp., and a mixture thereof, and using a ultra-high pressure extraction method to treat the fermentation product under 100-3,000 MPa at 40-90°C for 1-24 hours. According to the present invention, the cosmetic composition has excellent antioxidant effects and wrinkle-reducing effects.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a fermented ultra-high-pressure extract of narcissus scales,

The present invention relates to a cosmetic composition containing a daffodil stem extract and a fermented ultra-high-pressure extract, and more specifically, to a cosmetic composition having excellent antioxidative and wrinkle-reducing effects.

It is called aging of the skin through type and intangible changes that appear on the skin as the age goes on. In 2000, Korea became an aging society with more than 7% of the population aged 65 or older. In 2026, the population aged 65 or older is expected to enter the super-aged society of more than 20% The elderly as well as the younger generation are significantly more exposed to sunlight than previous generations, so care for skin aging and preventive measures against aging are needed more.

Skin is a protective membrane with various physiological functions and at the same time, it is an important organ for obtaining beauty appeal from others. Aging skin is accompanied by various functional dysfunctions of the skin with visible changes. On the other hand, elderly people with skin that looks younger than age are known to show more satisfaction in life. This is as important as the physiological function of the skin. Signs of severe aging have a profound effect on the vitality of life and can lead to depression in severe cases. Therefore, more aggressive efforts are needed to prevent or minimize skin aging.

Skin aging occurs in two main stages. Changes that occur with age without any special environmental factors are called intrinsic aging. The changes that appear on the face, neck, and back of a person exposed to environmental factors such as sunlight are referred to as photoaging.

The main cause of endogenous aging is the accumulation of damage to the constituents of our body caused by reactive oxygen radicals produced in the metabolic process of our body. Exogenous aging is different in that harmful free radicals are produced by ultraviolet rays. Once active oxygen is produced, it can not be effectively removed by various protective devices in the body, resulting in a series of inflammatory reactions that can lead to skin damage.

Photoaging is a major cause of skin aging and is responsible for most cosmetic and medical problems on the skin. The action spectrum of sunlight that causes photoaging is the ultraviolet region. Sunlight is mainly caused by ultraviolet B (UVB, 290 to 320 nm), but it causes skin aging and cancer as well as ultraviolet B and ultraviolet A (UVA, 320 to 400 nm). Therefore, ultraviolet rays A and B must be cut off to prevent photoaging.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness. In addition, there is a phenomenon in which fine wrinkles occur on the skin and the elasticity decreases due to aging. For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

To date, substances known to have antioxidant activity include ascorbic acid, dl-alpha-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT), and the like .

However, since these substances are used as synthetic products, they may cause problems of skin safety when they are used for a long period of time or in a large amount. Therefore, there is an urgent need to develop new antioxidative cosmetics that can be applied safely and effectively to skin. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

The name of daffodil is said to mean that it grows well in a place with moisture and likes water very much. Therefore, it is also called the Chinese lantern or the daffodil. It is native to the Mediterranean. The stem of the scales is wide egg-shaped and the skin is black. The leaves begin to grow in the late fall, are in the shape of a line, 20 ~ 40cm in length, 8 ~ 15mm in width, with dull end and greenish white.

Flowers bloom in December-March, length of tubers is 18 ~ 20mm, and peduncles are 20 ~ 40cm high. Bracts are membranous and enclose buds, and five to six flowers bloom at the end of the peduncle. There are 6 pieces of crustacean and white, and the hatching tube is 4mm high and yellow. Six stamens run under the horns, pistils can not bear fruit, and they multiply on scales.

The juice of daffodils is changed to cure the bruises, and the flowers are made of perfume to remove the wind. Scale stem is used medicinally such as geodam, pertussis.

Professor Ahn Duk-gyun's book, "Korea's first basics book," uses daffodil roots and flowers as medicines. It has the effect of sticking the root on the boil, and melting the fish thorn in the neck. Flowers resolve the fever of women (苦热 症).

Folk remedies can be applied by applying dough to the affected area by grinding the stem of the daffodil to the steel plate, such as arthritis, neuralgia, mastitis. In addition, the juice was applied to the gum, and it was used for pertussis, asthma, and vomiting.

The stem of the narcissus scales is called a bulb. The stem of the stem of the narcissus is a layer of scales formed by the base of the leaf. Leaves, stems, and roots are specially enlarged to store nutrients, and are an organ of roots developed into a form that looks like the tissue of the root.

Korean Patent Laid-Open Publication No. 2016-0114794 discloses a cosmetic composition for moisturizing the skin, a cosmetic composition for improving skin elasticity or improving wrinkles, which comprises a solution of a solution of the above-mentioned extracts, have.

Korean Patent Laid-Open Publication No. 2016-0021371 discloses a skin external composition for improving skin containing plant cells or adventitious roots of daffodil plants or extracts thereof.

Accordingly, the present inventors intend to provide a cosmetic composition containing a flavonoid component at a higher yield than the conventional art, and having excellent antioxidative and wrinkle-reducing effects.

To achieve the above object, the present invention provides a cosmetic composition having an antioxidative effect or a wrinkle-reducing effect, which comprises fermenting a stem of a daffylate scavenger, subjecting it to ultra-high pressure treatment and solvent extraction,

Preferably, the fermentation is carried out by fermenting the stem of the daffylate scales with a bacterium selected from the group consisting of yeast, lactic acid bacterium, Bacillus subtilis, and mixtures thereof while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C for 1 to 7 days By weight of the cosmetic composition.

Further, preferably, the cosmetic composition is characterized in that the yeast is Saccharomyces cerevisisae.

Preferably, the cosmetic composition is obtained by treating the ultra-high pressure treatment at a pressure of 100 to 3000 MPa at a temperature of 40 to 90 DEG C for 1 to 24 hours.

Preferably, the extraction is carried out in the presence of one or more selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And extracting the mixture using a solvent.

In addition, preferably, the daffylabicinal stem fermentation ultra-high pressure extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

The present invention can provide a cosmetic composition having an excellent antioxidative effect and a wrinkle-reducing effect by increasing the content of flavonoids by ultra-high pressure treatment and solvent extraction after fermentation of the stem of the narcissus scales.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term " drug " is used in reference to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight, or length of 30, 25, 20, 25, 10, 9, 8, 7, 6, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a sample,

Throughout this specification, the words " comprising " and " comprising ", unless the context clearly requires otherwise, include the steps or components, or groups of steps or elements, And that they are not excluded.

The inventors of the present invention have investigated the possibility of application as a cosmetic composition containing a fermented extract of a daffodil scales stem among various natural products, and after various experiments, it has been found that antioxidative and wrinkle-reducing effects can be expected and the efficacy as a cosmetic product can be expected. The ingredients of the flavonoid having such excellent effects were obtained at the highest possible yields, and a cosmetic composition having more excellent effects was developed.

The present invention relates to a cosmetic composition containing a daffylate scaly stem fermentation ultra-high pressure extract obtained by fermentation, ultra-high pressure treatment and solvent extraction of a stem of a daffylate scales, and more particularly, to a cosmetic composition comprising a stem of a daffylate scales as a yeast, a lactic acid bacterium, Wherein the fermented microorganism is fermented using a fermenting microorganism selected from the group consisting of a fermented microorganism selected from the group consisting of soybean,

In order to obtain a flavonoid component having various effects at the highest possible yield, the present invention was subjected to ultrahigh pressure treatment and extraction after fermentation of the stem of a daffodil scales. By this treatment, it was confirmed that the content of active ingredient was increased, and it was confirmed that antioxidative effect and wrinkle-reducing effect were obtained by applying it to cosmetics, thereby completing the present invention.

In the cosmetic composition of the present invention, the fermentation ultra high pressure extract of daffylate scales stem fermentation can be carried out in the presence of at least one bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus sp. 5 to 7, and fermentation is carried out for 1 to 7 days. More preferably, fermentation is carried out using Saccharomyces cerevisisae, and most preferred is saccharomyces cerevisiae KCTC 7904 And fermentation is carried out using a strain.

The Narcissus scales stem used in the present invention was prepared by a generally known method, and they were thoroughly washed, then milled and finely ground using 300 mesh. The pulverized narcissus scales stem was added to the culture medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) at a concentration of 100 g / L. 1 to 4% (W / V), preferably 1 to 2% (W / V) of glucose was added as a carbon source and 0.1 to 1% (W / V) W / V). The cultivation was carried out using a 5 L fermenter at a temperature of 20 to 40 ° C and for 1 to 7 days while maintaining the pH at 5-7. After culturing, the culture was centrifuged to remove the cultures first.

The ultra-high pressure treatment is carried out at a pressure of 100 to 3000 MPa at a temperature of 40 to 90 DEG C for 1 to 24 hours, preferably at a pressure of 500 to 1000 MPa at a temperature of 40 to 60 DEG C for 12 to 24 hours , Most preferably at a pressure of 1000 MPa and a temperature of 50 < 0 > C for 24 hours.

Preferably, the solvent extraction is carried out in the presence of a solvent selected from the group consisting of purified water, one selected from the group consisting of methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, The extract is preferably extracted using ethanol, 70% ethanol aqueous solution or water, and most preferably 70% ethanol aqueous solution.

Ethanol was added to the ultrafast processed daffylate scales stem fermentation extract to make a final 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, concentrated under reduced pressure, and lyophilized. Concentration under reduced pressure was carried out using a rotary vacuum concentrator. Concentration conditions were as follows: 700 ml of extract was put into a 1,000 ml round-bottomed flask, and the mixture was concentrated under reduced pressure at 650 to 750 mmHg at 50 to 70 ° C and 600 to 1200 rpm for 2 hours Respectively. The concentration of the concentrated solution was reduced to -40 ° C by preliminary freezing, maintained at -40 ° C for 2 to 4 hours, then dropped to -80 through trap freezing, the vacuum was maintained at 10 to 30 mTorr, Min up to -40 ° C. Then, the temperature was maintained at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, at 30 ° C for 130 minutes, and then maintained at 1300 minutes until completely dried.

The fermented ultra-high pressure extract of the narcissus scales may be an extract obtained by fermenting the fermented product obtained by fermenting the stem of the narcissus with a solvent and then extracting the extract by vacuum, concentrating and freeze-drying the extract, or a combination of the powder and an extraction solvent .

The extract of the daffylate stem fermentation ultra high-pressure extract is not limited to a specific range, but may be contained in an amount of preferably 0.0001 to 30% by weight, more preferably 0.1 to 10% by weight, based on the total weight of the cosmetic composition .

When the content of the extract is less than 0.0001% by weight, the effect of improving wrinkles is not exhibited. When the content of the extract is more than 30% by weight, the effect of increasing the content of the extract is insignificant, and there is a problem in safety and stability.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

< Example  1> Manufacture of Daffodil Scale Stem Fermented Extra-high Pressure Extract

Narcissus scales stem was selected, thoroughly washed, and finely pulverized to pass through a 300-mesh sieve.

The pulverized narcissus scales stem was added to the culture medium of Saccharomyces (Saccharomyces cerevisiae KCTC 7904) to be 10 g / L. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The culture was incubated for 1 to 7 days, preferably for 5 days, while maintaining the pH at 5-7, preferably at pH 6, using a 5 L fermenter at 37 ° C. After culturing the microorganism, the culture was centrifuged and the culture was firstly removed.

The fermented stem of the daffodil scales stemmed from the culture was first heated to 50 ° C under a pressure of 1000 MPa using an ultrahigh pressure processor, treated at an ultra-high pressure for 24 hours, and cooled to room temperature.

Ethanol was added to the ultrahigh pressure spermatogonial stem fermentation product to make a final 70% (v / v) ethanol solution, refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper.

When the filtration is completed, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure at 50 DEG C or below, followed by lyophilization. The lyophilized product was dissolved in 2% of 50% glycerin to prepare a daffodil scales stem fermentation ultra-high pressure extract (Example 1).

< Comparative Example  1> Daffodil scaly stem extract preparation

Comparative Example 1 was a general organic solvent extract of the stem of the narcissus, and the stem of the narcissus was selected by a sample, washed thoroughly and then pulverized and passed through a 300-mesh sieve to finely crush the product. Ethanol was added to make a final concentration of 70% (v / v) ethanol aqueous solution, refluxed three times for 5 hours, cooled down, and filtered through a filter of whatman # 2. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved in 50% glycerin to 2% (W / V) to prepare a Narcissus scales stem extract (Comparative Example 1).

&Lt; Comparative Example 2 > Production of fermented extract of stem of Narcissus scales

Comparative Example 2 was prepared by fermenting the stem of the narcissus scales. The stem of the narcissus was selected as a sample, thoroughly washed, and finely pulverized so as to pass through a 300-mesh sieve. This was added to the culture medium of Saccharomyces sp. (Saccharomyces cerevisiae KCTC 7904) to make 10 g / L of the stem of the daffodil scales. 2% (W / V) of glucose was added as a carbon source and 0.5% (w / v) of peptone was further added thereto. The culture was incubated for 1 to 7 days, preferably 5 days, while maintaining the pH at 5-7, preferably at pH 6, at 37 ° C in a 5 L fermenter. After culturing, the culture was centrifuged to remove the cultures first.

Ethanol was added so that the fermented stem of the daffylate scales, which had been firstly removed from the culture, was added to the final 70% (v / v) ethanol solution, refluxed three times for 5 hours, cooled, Filtered. After the filtration was completed, the extract was transferred to a thickener, and the filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved in 50% glycerin to 2% (W / V) to prepare a fermented extract of Daffodil stalks (Comparative Example 2).

&Lt; Comparative Example 3 > Preparation of Extra High Pressure Extract of Stem Stalks of Narcissus

The stem of the daffylate scales was thoroughly washed with a sample, washed thoroughly, and then finely pulverized so as to pass through a 300-mesh sieve, the temperature was raised to 50 ° C under a pressure of 1000 MPa using an ultra-high pressure processor, Lt; / RTI &gt;

Ethanol was added to the ultrasound-treated daffylate scales stem extract to make a final 70% (v / v) ethanol solution, refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. When the filtration is completed, the extract obtained in the extracting step is transferred to the concentration tank, and the filtered extract is concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a high-pressure extract of the narcissus scaly stem (Comparative Example 3).

<Experimental Example 1> Measurement of the active ingredient content of the stem of the narcissus scales

The content of each flavonoid in the stem of the narcissus of Example 1 and Comparative Examples 1 to 3 was analyzed and the results are shown in Table 1 below.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 content Flavonoid 1.89 1.2 1.35 1.31 %

As a result of measuring the active ingredient content of the narcissus stem of Example 1 and Comparative Examples 1 to 3, it was confirmed that the flavonoid component content of Example 1 was significantly higher than that of Comparative Examples 1 to 3.

From the above results, it can be seen that the extract of the stem of Narcissus scuta extracted in the processing condition (Example 1) in which the stem of the present invention is extracted by fermentation and subjected to high pressure treatment is compared with other extraction conditions (Comparative Examples 1 to 3) .

<Experimental Example 2> Measurement of antioxidative effect

The antioxidative activities of the extracts prepared in Example 1 and Comparative Examples 1 to 3 of the present invention were measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction . That is, Example 1 and Comparative Example 1 were carried out in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 占 퐇 of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 占 퐉 ol of ferrous chloride and 2 占 퐉 ol of hydrogen peroxide The dimethylsulfoxide (DMSO) test solution of the extract prepared in step 3 was added, and the mixture was incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. Add 0.3 ml of this solution to a test tube, add 3.0 ml of 0.05 N hydrochloric acid, 0.67% thiobarbituric acid (TBA) solution, and boil for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following formula.

The results are shown in Table 2 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Example 1 32 57 69 Comparative Example 1 21 30 41 Comparative Example 2 25 34 48 Comparative Example 3 24 35 47

The antioxidative effects of the extracts of the narcissus scales of Example 1 and Comparative Examples 1 to 3 were mostly high, and the extracts of the narcissus scales of Example 1 and Comparative Examples 1 to 3 and the conventional antioxidants ascorbic acid, The antioxidative activities of dl-alpha-tocopherol, beta-carotene and dibutylhydroxyltoluene (BHT) were measured by the same method as Experimental Example 2 to compare IC ₅₀ as a 50% inhibitory concentration Respectively.

The results are shown in Table 3 below.

Test substance IC ₅₀ (10 μg / ml) Example 1 30.7 Comparative Example 1 44.5 Comparative Example 2 38.8 Comparative Example 3 37.2 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The Narcissus scallop extracts of Example 1 and Comparative Examples 1 to 3 showed comparable high activity to other antioxidants. In particular, it can be seen that the extract of the present invention has an extremely high antioxidative activity despite being a mixture of natural products.

Formulation Example 1 and Comparative Formulation Examples 1 to 3

The prescription examples of the cream in the cosmetic containing the narcissus scaly stem extract are shown in Table 4 below. The extracts of the narcissus scales of Example 1 and Comparative Examples 1 to 3 were used.

Component (% by weight) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 The extract of Example 1 5.0 - - - The extract of Comparative Example 1 - 5.0 - - The extract of Comparative Example 2 - - 5.0 - The extract of Comparative Example 3 - - - 5.0 glycerin 5.0 5.0 5.0 5.0 Sodium hyaruronate 4.0 4.0 4.0 4.0 Polysorbate 60 1.5 1.5 1.5 1.5 Mineral oil 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 Dimethicone 2.0 2.0 2.0 2.0 Triethanolamine 1.0 1.0 1.0 1.0 Chin type monostearin glycerin 2.0 2.0 2.0 2.0 Cetearyl alcohol 2.2 2.2 2.2 2.2 Stearic acid 1.5 1.5 1.5 1.5 Sorbitan stearate 0.6 0.6 0.6 0.6 Sodium magnesium silicate 0.1 0.1 0.1 0.1 Betaine 3.0 3.0 3.0 3.0 antiseptic a very small amount a very small amount a very small amount a very small amount incense a very small amount a very small amount a very small amount a very small amount Pigment a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance system 100 100 100 100

< Experimental Example  3> Evaluation of Wrinkle Reduction Effect

The effect of improving the wrinkles of the daffodil scales stem fermented ultra-high pressure extract cream (Formulation Example 1) and Comparative Formulation Examples 1 to 3 was evaluated as follows.

Forty subjects (35 to 50 year-old women) of the experiment used the creams prepared in Formulation Example 1 on the right side of the face and the creams of Comparative Formulation Examples 1 to 3 on the left side of the face twice a day The wrinkle improvement effect after 6 weeks was visually evaluated to confirm the wrinkle improvement degree. The results of wrinkle improvement based on this evaluation are shown in Table 5 below.

Experimental product Great slightly none Effective rate (%) Formulation Example 1 8 2 0 88.3 Comparative Formulation Example 1 4 4 2 58.4 Comparative Formulation Example 2 6 2 2 66.5 Comparative Formulation Example 3 5 3 2 61.7

As shown in Table 5, it was found that the skin wrinkle-improving effect of the applicator coated with the cream containing the daffylate scales stem fermentation ultra-high pressure extract was excellent. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition having an antioxidative effect, which comprises fermenting a stem of a daffodil scales, followed by ultra-high pressure treatment and solvent extraction, and extracting a daffodil scales stem fermentation ultra-high pressure extract. A cosmetic composition having a wrinkle-improving effect, comprising a fermented ultra-high-pressure extract of a daffodil scales stem obtained by fermenting stem of a daffodil scales, followed by ultra-high pressure treatment and solvent extraction. 3. The method according to claim 1 or 2,
The fermentation is carried out by fermenting the stem of the daffylate scales for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 DEG C by at least one bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis, &Lt; / RTI &gt; 3. The method according to claim 1 or 2,
Wherein the ultra high pressure treatment is performed at a pressure of 100 to 3000 MPa and a temperature of 40 to 90 DEG C for 1 to 24 hours. 3. The method according to claim 1 or 2,
The solvent extraction may be carried out using at least one solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And extracting the cosmetic composition. 3. The method according to claim 1 or 2,
The cosmetic composition according to any one of claims 1 to 3, wherein the Dextrose Stem Fermentation Ultra High Pressure Extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.