KR20180002158A - A composition for removing hangover comprising an herb extract including ginseng - Google Patents

Abstract

The present invention relates to a composition for relieving hangover comprising a medicinal herb extract including ginseng as an active ingredient. The composition hinders alcohol dehydrogenase (ADH) activity and promotes aldehyde dehydrogenase (ALDH) activity, thereby inhibiting formation of acetaldehyde which causes side effects to the human body.

Description

[0001] The present invention relates to a composition for removing hangovers containing an extract of a medicinal herb containing ginseng as an active ingredient,

The present invention relates to a method for producing an acetaldehyde which inhibits alcohol dehydrogenase (ADH) activity and accelerates aldehyde dehydrogenase (ALDH) activity by containing an extract of herbal medicines containing ginseng as an active ingredient, To the composition for relieving hangover.

Alcohol ingested into the body is absorbed 20% in the stomach and 80% in the small intestine. The rate of absorption depends on the type of alcohol, but generally the higher the alcohol concentration, the faster the alcohol is consumed fast. In the stomach and intestines, the absorbed alcohol spreads throughout the body along with the blood, which is decomposed while passing through the liver.

The alcohol metabolism process in the liver is firstly oxidized to acetaldehyde by an alcohol dehydrogenase (ADH), and the acetaldehyde is decomposed into acetic acid (CH ₃ COOH) by an aldehyde dehydrogenase, It is exhausted out, it circulates in the body and is decomposed again into water (H ₂ O) and carbon dioxide (CO ₂ ).

Specifically, enzymes involved in the process of decomposing alcohol into acetaldehyde include alcohol dehydrogenase (ADH) as well as microsomal ethanol oxidase (MEOS). The alcohol dehydrogenase (ADH) accounts for about 80 to 90% of the total alcohol treatment, and the microsomal ethanol oxidase enzyme (MEOS) treats the remaining 10 to 20%. In addition, the acetaldehyde produced by the alcohol dehydrogenase (ADH) and the microsome ethanol oxidase enzyme (MEOS) is converted into acetic acid which does not harm the human body by the aldehyde dehydrogenase (ALDH) .

The acetaldehyde is toxic and is sent from the heart to the coronary artery. It is impregnated into muscular or fatty tissue throughout the body through tightly woven blood vessels like spider webs. In this process, acetaldehyde impacts the blood vessels. When the alcohol is moderately drunk, such shocks expand the blood vessels to help flow blood. However, alcohol and acetaldehyde, If you turn your body around in your blood, it will be harmful to many organs of your body.

In particular, the acetaldehyde stimulates the afferent nerve fibers in the vagus nerve and the sympathetic nerve to cause an alcohol effect (hangover) such as vomiting, dizziness, enlargement of the pupil, heartbeat, and breathing. Studies have shown that acetaldehyde is highly toxic, leading to cancer in animal experiments.

Therefore, there is a demand for a composition capable of inhibiting the production of acetaldehyde to minimize liver damage.

Korea Patent Publication No. 2014-0091850 Korea Patent Publication No. 2003-0018127 Korean Patent No. 1447231

It is an object of the present invention to provide a method for inhibiting alcohol dehydrogenase (ADH) activity and promoting aldehyde dehydrogenase (ALDH) activity by containing an extract of herb medicines containing ginseng as an active ingredient, And to inhibit the production of acetaldehyde causing side effects.

Another object of the present invention is to provide a health functional food for hangover resolution.

To achieve the above object, the composition for relieving hangover of the present invention may contain, as an active ingredient, a herbal medicine extract containing a herbal medicine containing ginseng, broiler chick, and Angelica gigantosa.

The ginseng may be at least one selected from the group consisting of Taicha ginseng, white ginseng, red ginseng and black ginseng.

The ginseng, broiler chicks and Angelica gigas can be mixed at a weight ratio of 1: 1-7: 1-7.

The medicinal herb may further include at least one selected from the group consisting of garlic cloves, clams and parasites.

5 to 50 parts by weight of chopped garlic, 5 to 50 parts by weight of clams and 5 to 50 parts by weight of paraffin can be further added to 100 parts by weight of the herbal medicine.

The medicinal herb extract may be extracted by mixing the medicinal herb with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof in a weight ratio of 1: 0.5 to 10.

The extraction temperature may be 50-100 < 0 > C.

In order to accomplish the above-mentioned other objects, the health functional food for hangover resolution of the present invention may contain, as an active ingredient, an extract of herbal medicines containing herbal medicines containing ginseng, broiler chicks and Angelica gigas.

The health functional food may be selected from the group consisting of capsules, tablets, powders, granules, liquids, rings, flakes, pastes, syrups, gels, jellies, and the like.

The composition for relieving hangover of the present invention inhibits the activity of alcohol dehydrogenase (ADH) to inhibit the production of acetaldehyde, which causes adverse effects on the human body. In addition, a part of acetaldehyde produced promotes the activity of aldehyde dehydrogenase (ALDH) It is metabolized by acetic acid.

In addition, since the composition for relieving hangover of the present invention is free of toxicity, it can be used as a health functional food.

The present invention relates to inhibition of alcohol dehydrogenase (ADH) activity by promoting the activity of aldehyde dehydrogenase (ALDH) by inhibiting alcohol dehydrogenase (ADH) activity and inhibiting the production of acetaldehyde, To a composition for relieving hangover.

Hereinafter, the present invention will be described in detail.

The composition for relieving hangover of the present invention contains an extract of a medicinal herb containing an oriental medicinal herb including ginseng, broiler chick, and Angelica gigantosa as an active ingredient. The ginseng may be at least one selected from the group consisting of Taicha ginseng, white ginseng, red ginseng and black ginseng.

The Taegeuk ginseng is ginseng which is prepared by boiling unscratched ginseng in hot water at 80 to 85 캜 for 20 to 25 minutes and then being dried in an upright form and processed into an intermediate form of red ginseng and white ginseng. In addition, the Health Function Food Act can display the immunity enhancement and fatigue elimination functionality.

In addition, the white ginseng is ginseng which is prepared by removing the epidermis from the ginseng of more than 4 years old muscle as raw material and drying the ginseng so that the moisture content becomes 14% or less. As in the case of the ginseng tea ginseng, The fatigue resolution function can be displayed.

In addition, the red ginseng is steamed green ginseng and dried red ginseng. Its functionality based on the Health Functional Food Act helps blood flow by increasing immunity, relieving fatigue, improving memory, inhibiting platelet aggregation, Lt; / RTI >

In addition, the black ginseng refers to ginseng produced by repeating the process of growing and drying ginseng three times or more, and is called black ginseng. Recently, the Korea Food and Drug Administration (KFDA) classifies black ginseng as a type similar to red ginseng, and its functionality based on the Health Functional Food Act helps blood flow by increasing immunity, relieving fatigue, improving memory, inhibiting platelet aggregation, and exhibiting antioxidant activity .

In addition, the broiler meat refers to the bark of Cinnamomum loureiriie (broth). The broiler is spicy and sweet, and it is a medicinal material that is good for the good blood circulation of our body because it is hot, and it has the function of inhibiting the central nervous system, calming, analgesic, fever, increasing the blood flow of the coronary artery and inhibiting the skin fungus, , Frequent dilution of urine, diarrhea, vomiting, menstrual irregularities, menstrual cramps, postpartum hemorrhagic abdominal pain, boils, skin ulcers, and limb paralysis.

In addition, the above refers to the root of the perennial oriental herbarium Angelica gigas belonging to the falciparum gland, which shows high anticancer activity in stomach cancer and lung cancer cells, and also has a promoting effect of normal hepatocytes and also an inhibitory activity function. In addition, Angelica gangrene lowers the blood cholesterol level and lowers the triglyceride and total fat content of the liver, which is increased by alcohol administration, to prevent liver damage due to chronic alcohol consumption.

To prepare the medicinal herb extract, firstly medicinal herbs including Taegeuk ginseng, broiler ginseng and Angelica gigas are washed with water and dried.

The herbal medicines are mixed at a weight ratio of 1: 1-7: 1-7, preferably 1: 1-3: 1-3, by weight of ginseng, broiler chicks and Angelica gigas. When the content of broiler chicks and Angelica gigas is less than the lower limit, the alcohol dehydrogenase (ADH) inhibitory activity is low and the activity of the aldehyde dehydrogenase (ALDH) is not promoted. On the other hand, when the content of the alcohol dehydrogenase ) Inhibitory activity may be lowered.

The medicinal herb may further comprise at least one additive selected from the group consisting of 5 to 50 parts by weight of ground garlic, 5 to 50 parts by weight of clam, and 5 to 50 parts by weight of parchment, relative to 100 parts by weight of the medicinal herb. In the case of not further containing one or more additives selected from the group consisting of garlic cloves, cloves and parasites, the activity of aldehyde dehydrogenase (ALDH) may not be further promoted.

When the content of at least one selected from the group consisting of the above-mentioned chopped garlic, clam, and paraffin is less than the lower limit, the activity of the aldehyde dehydrogenase (ALDH) may not be further promoted. When the content is above the upper limit, And the alcohol dehydrogenase (ADH) inhibitory activity may be lowered.

The medicinal herb (or mixture of herbal medicines and additives) and the extraction solvent are mixed at a weight ratio of 1: 0.5 to 10, preferably 1: 0.5 to 7, at 50 to 100 ° C, preferably 60 to 90 ° C for 10 minutes To 10 hours, preferably 2 to 8 hours, to prepare an extract.

The extraction solvent is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably water. The mixed solvent is not particularly limited, but methanol, ethanol, butanol or propanol of 20 to 80% is exemplified.

When the weight ratio of the herbal medicine (or the mixture of herbal medicine and the additive) to water or the alcohol is out of the range, the effective amount of the herbal medicine (or the mixture of the herbal medicine and the additive) in the extract may be extracted in a small amount.

The herbal medicine extract prepared as described above may be used as an extract itself or may be powdered for convenient use. The method for pulverizing the herbal medicine extract is lyophilized and lyophilized by a freeze dryer.

The composition containing the medicinal herb extract of the present invention as an active ingredient to inhibit the production of acetaldehyde and capable of eliminating the hangover can be obtained by extracting the medicinal herb extract in an amount of 0.01 to 80% by weight, preferably 0.1 to 50% by weight, .

The composition of the present invention can be formulated into various oral or parenteral administration forms.

Examples of formulations for oral administration include tablets, pills, hard, soft capsules, liquids, suspensions, emulsions, syrups, granules and the like. These formulations may contain, in addition to the active ingredient, a diluent such as lactose, dextrose, (For example, silica, talc, stearic acid and magnesium or calcium salts thereof and / or polyethylene glycol), in addition to the active ingredient (s). The tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and / or polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid Or a disintegrating or boiling mixture such as its sodium salt and / or an absorbent, a colorant, a flavoring agent and a sweetening agent. The formulations may be prepared by conventional mixing, granulating or coating methods.

Representative examples of formulations for parenteral administration include injectable preparations, and water, Ringer's solution, isotonic saline or suspensions may be mentioned as a solvent for the injectable preparation. The sterile, fixed oils of the injectable preparations may be used as a solvent or suspending medium, and any non-irritating fixed oils, including mono-, di-glycerides, may be used for this purpose. The injectable preparation may be a fatty acid such as oleic acid.

Although the dose of the composition for hangover decontamination containing the herbal medicine extract of the present invention as the active ingredient varies depending on the age, sex, weight and severity of the disease of the patient, the composition of the present invention is not more than 300 mg / Preferably, 10 to 200 mg is administered 1 to 4 times a day, but is not limited thereto.

The health functional food for hangover poultry containing the herbal medicine extract of the present invention as an active ingredient contains 0.01 to 80% by weight of herbal extracts based on the total weight of the health functional food.

The health functional food may be in the form of a capsule, tablet, powder, granule, liquid, ring, flaky, paste, syrup, gel, jelly and jute for the purpose of supplementing nutrients that may be lacking in daily eating or supplementing functional raw materials useful in the human body And is easily manufactured and operated once.

In addition, the health functional food may contain at least one selected from the group consisting of glucose, citric acid, liquid oligosaccharide, corn syrup, soybean lecithin, butter, vegetable hardening oil, skim milk, sugar, margarine, salt, starch, And can be manufactured by adding conventionally used components such as sodium bicarbonate and sugar ester, and additionally, for the purpose of preventing liver damage, by adding hematocrit (Hovenia dulcis) and / or silymarin (milk seed).

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

≪ Embodiment 1 >

Examples 1-4.

Each material and purified water were mixed with aluminum foil according to the contents of the following Table 1, hot-water-extracted at 80 ° C for 8 hours at atmospheric pressure, and mixed with water to prepare a water extract. The unit is g.

division Example 1 Example 2 Example 3 Example 4 Tai Chi Gam 33.4 - - - White ginseng - 33.4 - - Red ginseng - - 33.4 - Black - - - 33.4 Broiler 33.3 33.3 33.3 33.3 Angelica 33.3 33.3 33.3 33.3 Total weight of medicinal herbs 100 100 100 100

< Test Example _1>

In the extracts prepared in Examples and Comparative Examples, 0.1 ml of the extract was added to the reaction solution when ADH inhibitory activity and ALDH activity were measured.

Test Example 1. Measurement of alcohol dehydrogenase ( ADH ) activity

Preincubation of the test tube with distilled water, buffer (0.1 M phosphate buffer, pH 7.0), NAD ⁺ (6.25 mg / mL buffer soln.) And ADH (3 mg / mL buffer soln. Ethanol was added thereto, and the reaction was carried out at 30 ° C for 5 minutes. After completion of the reaction, the reaction was cooled with ice water, and the absorbance at 340 nm was measured to find that the alcohol dehydrogenase Activity (%) was measured.

The inhibitory activity measurement conditions are shown in Table 2 below, and the results are shown in Table 3.

[Equation 1]

(%) = {[(T-CC) - (T-TC) / C-CC]

T: Absorbance of the sample, TC: Absorbance of the sample control

C: absorbance of enzyme reaction sphere, CC: absorbance of sample and enzyme reaction sieve

division T TC C CC Distilled water (ml) 0.5 0.5 0.5 0.6 Buffer 0.5 0.6 0.6 0.6 NAD 0.15 0.15 0.15 0.15 ADH 0.1 - 0.1 - extract 0.10 0.10 - - Ethanol (99.9%) 0.15 0.15 0.15 0.15

division Example 1 Example 2 Example 3 Example 4 ADH activity
(%) 0.1 0.2 0.2 0.3

As shown in Table 3 above, it was confirmed that the ADH activity of Examples 1 to 4 was nearly 0%.

Test Example  2. Aldehyde dehydrogenase ( ALDH ) Active measurement

To the test tube was added distilled water, buffer (1M-Tris-HCl, pH 8.0), 3M-KCl, 0.33M-2-Mercaptoethanol, NAD ⁺ (20 mM, 6.75 mg / ml in buffer) and ALDH (2.5 mg / ) And extracts were reacted at 25 ° C. for 5 minutes, and acetaldehyde (1 M) was added thereto. The reaction was carried out at 25 ° C. for 5 minutes. After completion of the reaction, the reaction mixture was cooled with ice water and then absorbance was measured at 340 nm The activity (%) was measured according to the following formula (2), and the activity (%) of the [Table 5] represents the relative activity (%) relative to the control.

The activity measurement conditions are shown in Table 4 below, and the results are shown in Table 5.

&Quot; (2) &quot;

(%) = {[(T-CC) - (T-TC) / C-CC]

T: Absorbance of the sample, TC: Absorbance of the sample control

C: absorbance of enzyme reaction sphere, CC: absorbance of sample and enzyme reaction sieve

division T TC C CC Distilled water (ml) 0.5 0.5 0.5 0.55 Buffer 0.5 0.55 0.55 0.55 3M-KCl 0.1 0.1 0.1 0.1 0.33M-2-mercaptoethanol 0.1 0.1 0.1 0.1 NAD 0.1 0.1 0.1 0.1 ALDH 0.05 - 0.05 - extract 0.05 0.05 - - Acetaldehyde 0.1 0.1 0.1 0.1

division Example 1 Example 2 Example 3 Example 4 ALDH
activation(%) 106.4 102.3 103.0 103.5

As shown in Table 5 above, it was confirmed that Example 1 using Taechuk ginseng as ginseng stimulated ALDH activity more than Examples 2 to 4.

Among the four kinds of ginseng, the efficacy of Taegeuk ginseng was excellent.

< Example  And Comparative Example _2>

Control group 1.

Water was used.

Example  And Comparative Example .

The procedure of Example 1 was repeated.

division Example 1 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Tai Chi Gam 33.4 33.4 33.4 25 25 20 20 5.8 - 50 50 50 Broiler 33.3 33.3 33.3 50 50 60 60 47.1 50 - 50 50 Angelica 33.3 33.3 33.3 25 25 20 20 47.1 50 50 - - Total weight of medicinal herbs
(Weight ratio) 100
(1: 1: 1) 100
(1: 1: 1) 100
(1: 1: 1) 100
(1: 2: 1) 100
(1: 2: 1) 100
(1: 3: 1) 100
(1: 3: 1) 100
(1: 8: 8) 100 100 100 100 chopped garlic - 33.3 33.3 - 33.3 - - - - - - 33.3 Clam - - 33.3 - - - - - - - - 33.3 Blue - - - - - - 33.3 - - - - 33.3 Purified water 400 400 400 400 400 400 400 400 400 400 400 400

< Test Example >

Test Example 3. Measurement of alcohol dehydrogenase ( ADH ) activity

The test was carried out in the same manner as in Test Example 1 to obtain an alcohol dehydrogenase Activity (%) was measured.

division Example 1 Example 5 Example 6 Example 7 Example 8 Example 9 ADH activity
(%) 23.4 17.2 16.7 0 0 0 division Example 10 Example 11 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 ADH activity
(%) 0 0 0 25.8 26.4 22.3

As shown in Table 7, it was confirmed that the extracts of Examples 7 to 11 of the present invention inhibited ADH activity by 100%.

Specifically, the extracts of Examples 1, 5 and 6 were obtained by mixing Taegeuk ginseng, broiler chickens, and Angelica gigas Nakai at a weight ratio of 1: 1: 1. Although the addition of additives did not inhibit ADH activity 100% 80%. &Lt; / RTI &gt; In addition, as in Comparative Examples 2 to 4, it was confirmed that ADH activity was inhibited by 70-80% when ADH activity was not inhibited by 100% when no one of Taegeuk ginseng, broiler ginseng and Angelica giganteus were used.

The ADH activity of the extracts of Taegeuk ginseng, broiler chicks, and Angelica gigantus were measured using a weight ratio of 1: 1: 2, a weight ratio of 1: 1: 3, a weight ratio of 1: 2: 2, and a weight ratio of 1: 8: It was confirmed that ADH activity was measured at 0%, thereby inhibiting ADH activity by 100%.

Test Example  4. Aldehyde dehydrogenase ( ALDH ) Active measurement

The activity of aldehyde dehydrogenase (%) was measured in the same manner as in Test Example 2 above.

division Example 1 Example 5 Example 6 Example 7 Example 8 Example 9 ALDH
activation(%) 106.4 107.8 114.1 110.7 121.8 111.9 division Example 10 Example 11 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 ALDH
activation(%) 127.1 83.6 74.6 78.4 84.8 87.2

As shown in Table 8 above, it was confirmed that the group to which the additives were added as a whole accelerated ALDH activity as compared with the group to which the additive was not added.

Specifically, it was confirmed that the addition of parasites among the additives accelerated the ALDH activity more than the addition of the minced garlic or clam.

It was confirmed that the extracts of Example 1 and Examples 5 to 10 of the present invention had an ALDH activity of 100% or more and promoted ALDH activity. However, Example 11 and Comparative Examples 1 to 4 showed that the ALDH activity was not promoted Respectively.

The ALDH activity of the extracts of Taegeuk ginseng, broiler chickens and Angelica gigas were measured using a weight ratio of 1: 1: 2, a weight ratio of 1: 1: 3, and a weight ratio of 1: 2: 2. As a result, 108.5%, 109.4% %, Indicating that ALDH activity was promoted.

According to the results measured in Test Examples 3 and 4, the extracts promoting the activity of the aldehyde dehydrogenase (ALDH) while inhibiting the activity of alcohol dehydrogenase (ADH) were obtained from the extracts of Examples 7 to 10 and the extracts of Tai Chi, : 1: 2, a weight ratio of 1: 1: 3, and a weight ratio of 1: 2: 2.

The following Test Example 5-7 was conducted using the extracts of Examples 7 to 10 and the extracts of Comparative Examples 1 to 4.

Test Example  5. Evaluation of alcohol decomposition

The extracts prepared in Examples 7 to 10 and Comparative Examples 1 to 4 were tested for their ability to decompose alcohol, and then the extracts were administered to the test animals together with the alcohol. After 1, 3, and 5 hours, Aldehyde concentration was measured to evaluate the alcohol degradation effect in the body.

Six-week old SD rats were administered 3 mL of alcohol per kg of body weight, and the test product was administered twice 30 minutes before and after alcohol administration. Blood alcohol concentration and acetaldehyde concentration after 0, 1, 3, and 5 hr of alcohol administration were measured. The results are shown in Table 5 below.

division Blood alcohol concentration (% alcohol) Acetaldehyde concentration (mg / L) 1 hr 3 hr 5 hr 1 hr 3 hr 5 hr Control 0.034 0.038 0.031 8.8 6.7 5.7 Example 7 0.065 0.048 0.036 1.0 2.0 1.1 Example 8 0.068 0.050 0.037 0.9 1.8 1.3 Example 9 0.067 0.049 0.035 0.9 1.9 1.2 Example 10 0.067 0.048 0.036 1.1 1.8 1.0 Comparative Example 1 0.047 0.038 0.029 2.1 2.8 2.4 Comparative Example 2 0.052 0.040 0.031 1.9 2.6 2.1 Comparative Example 3 0.050 0.039 0.030 1.7 2.2 1.8 Comparative Example 4 0.055 0.042 0.032 1.4 2.0 1.4

As shown in Table 9, the extracts of Examples 7 to 10 showed that the blood alcohol concentration was about twice as high as that of the control (based on 1 hour), and that the extracts of Comparative Examples 1 to 4 were 1.5 times higher than those of the control (1 hour basis).

On the other hand, since the extracts of Examples 7 to 10 and Comparative Examples 1 to 4 exhibit a much lower concentration of acetaldehyde than those of the control, the extracts of Examples and Comparative Examples strongly inhibit the activity of ADH while promoting the activity of ALDH Respectively.

Test Example  6. Measure respiratory alcohol concentration

Nine individuals of 20 to 25 years old were allowed to consume 2 ml / kg body weight of alcohol at an alcohol concentration of 20 ° C in 1 minute, and then 100 ml of the extract prepared in Examples 7 to 10 and Comparative Examples 1 to 4 was taken in an aerobic Respiratory alcohol concentration was measured 10, 60, and 120 minutes after alcohol consumption using a breathalyzer.

division Control Example 7 Example 8 Example 9 Example 10 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Breathing alcohol concentration (%) 10 minutes 0.017 0.023 0.023 0.022 0.024 0.019 0.018 0.018 0.019 60 minutes 0.019 0.032 0.031 0.033 0.030 0.028 0.025 0.027 0.026 120 minutes 0.007 0.013 0.015 0.013 0.014 0.015 0.017 0.017 0.018

As shown in Table 10, respiratory alcohol concentration was decreased in all the groups until 60 minutes, but after 90 minutes, respiratory alcohol concentration began to decrease.

The extracts prepared according to Examples 7 to 10 of the present invention were found to have a higher respiratory alcohol concentration than the control. It was confirmed that Comparative Examples 1 to 4 were also higher in respiratory alcohol concentration than the control group but lower than those of Examples 7 to 10.

That is, as a result of Test Examples 5 and 6, the extracts prepared according to Examples 7 to 10 showed a high alcohol content and a low acetaldehyde content.

Test Example  7. Stability measurement at pH 2

The extract prepared in Examples 7 to 10 and Comparative Examples 1 to 4 was adjusted to pH 2 with 35% HCl, treated at 37 ° C for 0.5 to 4 hours (in the gastrointestinal condition), and then treated with 36% NaOH To adjust the pH to 7.0, and the activity of alcohol dehydrogenase (ADH) was measured according to the method of Test Example 1, and the results are shown in Table 11 below.

division Example 7 Example 8 Example 9 Example 10 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 ADH
activation(%) 0.5 hr 1.0 1.1 1.3 1.5 32.2 25.5 28.2 22.2 1 hr 1.5 1.3 1.3 1.1 33.5 26.6 26.1 23.8 2 hr 1.4 1.2 1.2 0.9 34.1 25.0 27.4 23.0 4 hr 2.0 1.7 1.5 1.4 34.8 26.7 28.7 23.5

As shown in Table 11, the extracts of Examples 7 to 10 and Comparative Examples 1 to 4 were found to be stable with almost no change in ADH activity when they were treated at pH 2.0, 37 ° C, which is the gastrointestinal condition, for up to 4 hours .

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  One. Sanje  Produce

The extract powder obtained in Example 4                     500 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation example  2. Preparation of tablets

300 mg of the extract powder obtained in Example 4

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

200 mg of the extract powder obtained in Example 4

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

600 mg of the extract powder obtained in Example 4

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

It is prepared by the above-mentioned component content per ampoule according to the usual injection preparation method.

Formulation example  5. Liquid  Produce

4 g of the extract powder obtained in Example 4

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100g by adding purified water, To prepare a liquid agent.

Formulation example  6. Preparation of granules

1,000 mg of the extract powder obtained in Example 4

Vitamin mixture quantity

70 ug of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2 ug

Vitamin C 10 mg

Biotin 10 ug

Nicotinic acid amide 1.7 mg

Folic acid 50 ug

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above-mentioned vitamins and minerals is comparatively comparatively mixed with the granules according to the preferred embodiment. However, the blending ratio may be arbitrarily changed, and the above components are mixed according to the ordinary granule preparation method, Can be prepared and used in the manufacture of a health functional food composition according to a conventional method.

Formulation example  7. Manufacture of functional beverages

1,000 mg of the extract powder obtained in Example 4

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added and the entire 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the compounding ratio may be arbitrarily varied depending on the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (8)

A composition for removing hangover characterized by containing an extract of a medicinal herb containing a medicinal herb including ginseng, broiler chicks and Angelica gigas as an active ingredient. The composition for removing hangover according to claim 1, wherein the ginseng is at least one selected from the group consisting of Taegeuk ginseng, white ginseng, red ginseng and black ginseng. The composition for removing hangover according to claim 1, wherein the ginseng, broiler chicks and Angelica gigas are mixed at a weight ratio of 1: 1-7: 1-7. [5] The method according to claim 3, further comprising at least one selected from the group consisting of 5 to 50 parts by weight of chopped garlic, 5 to 50 parts by weight of clams and 5 to 50 parts by weight of parasitic to 100 parts by weight of the herbal medicine. / RTI &gt; The composition for relieving hangover according to claim 1, wherein the medicinal herb extract is mixed with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof at a weight ratio of 1: 0.5 to 10. The composition for removing hangover according to claim 1, wherein the extraction temperature is 50 to 100 ° C. A health functional food for relieving hangover characterized by containing an extract of a medicinal herb containing a medicinal herb including ginseng, broiler, and Angelica gigas as an active ingredient. The health functional food according to claim 7, wherein the health functional food is selected from the group consisting of capsules, tablets, powders, granules, liquids, rings, flakes, pastes, syrups, gels, .