KR20180002156A - A composition for removing hangover comprising an extract of Angelica gigas - Google Patents

Abstract

The present invention relates to a composition for removing hangover comprising an Angelica gigas extract as an active ingredient, which inhibits the activity of alcohol dehydrogenase (ADH) and promotes the activity of aldehyde dehydrogenase (ALDH) to inhibit the production of acetaldehyde, which causes side effects to the human body.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for removing hangovers containing Angelica gigantosa extract as an active ingredient,

The present invention relates to a method for inhibiting the production of acetaldehyde, which inhibits the activity of alcohol dehydrogenase (ADH) and promotes the activity of aldehyde dehydrogenase (ALDH) The present invention relates to a composition for dissolving.

Alcohol ingested into the body is absorbed 20% in the stomach and 80% in the small intestine. The rate of absorption depends on the type of alcohol, but generally the higher the alcohol concentration, the faster the alcohol is consumed fast. In the stomach and intestines, the absorbed alcohol spreads throughout the body along with the blood, which is decomposed while passing through the liver.

The alcohol metabolism process in the liver is firstly oxidized to acetaldehyde by an alcohol dehydrogenase (ADH), and the acetaldehyde is decomposed into acetic acid (CH ₃ COOH) by an aldehyde dehydrogenase, It is exhausted out, it circulates in the body and is decomposed again into water (H ₂ O) and carbon dioxide (CO ₂ ).

Specifically, enzymes involved in the process of decomposing alcohol into acetaldehyde include alcohol dehydrogenase (ADH) as well as microsomal ethanol oxidase (MEOS). The alcohol dehydrogenase (ADH) accounts for about 80 to 90% of the total alcohol treatment, and the microsomal ethanol oxidase enzyme (MEOS) treats the remaining 10 to 20%. In addition, the acetaldehyde produced by the alcohol dehydrogenase (ADH) and the microsome ethanol oxidase enzyme (MEOS) is converted into acetic acid which does not harm the human body by the aldehyde dehydrogenase (ALDH) .

The acetaldehyde is toxic and is sent from the heart to the coronary artery. It is impregnated into muscular or fatty tissue throughout the body through tightly woven blood vessels like spider webs. In this process, acetaldehyde impacts the blood vessels. When the alcohol is moderately drunk, such shocks expand the blood vessels to help flow blood. However, alcohol and acetaldehyde, If you turn your body around in your blood, it will be harmful to many organs of your body.

In particular, the acetaldehyde stimulates the afferent nerve fibers in the vagus nerve and the sympathetic nerve to cause an alcohol effect (hangover) such as vomiting, dizziness, enlargement of the pupil, heartbeat, and breathing. Studies have shown that acetaldehyde is highly toxic, leading to cancer in animal experiments.

Therefore, there is a demand for a composition capable of inhibiting the production of acetaldehyde to minimize liver damage.

Korean Patent Publication No. 2015-0017114 Korea Patent Publication No. 2002-0078467

It is an object of the present invention to suppress the production of acetaldehyde which inhibits alcohol dehydrogenase (ADH) activity and accelerates the activity of aldehyde dehydrogenase (ALDH) And to provide a composition for relieving hangover.

Another object of the present invention is to provide a health functional food for hangover resolution.

To achieve the above object, the composition for relieving hangover of the present invention may contain Angelica gigas extracts as an active ingredient.

The Angelica giganta extract may be obtained by mixing Angelica gigas Nakai, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof at a weight ratio of 1:10 to 30.

The mixed solvent may be 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol.

The extraction temperature may be 50-100 < 0 > C.

In order to achieve the above-mentioned other objects, the health functional food for hangover resolution of the present invention may contain the Angelica gigantosa extract as an active ingredient.

The health functional food may be selected from the group consisting of capsules, tablets, powders, granules, liquids, rings, flakes, pastes, syrups, gels, jellies, and the like.

The composition for relieving hangover of the present invention inhibits the activity of alcohol dehydrogenase (ADH) to inhibit the production of acetaldehyde, which causes adverse effects on the human body. In addition, a part of acetaldehyde produced promotes the activity of aldehyde dehydrogenase (ALDH) It is metabolized by acetic acid.

In addition, since the composition for relieving hangover of the present invention is free of toxicity, it can be used as a health functional food.

The present invention relates to a method for inhibiting the activity of alcohol dehydrogenase (ADH) and promoting the activity of aldehyde dehydrogenase (ALDH) to inhibit the production of acetaldehyde, which causes adverse effects on the human body, and the production of acetaldehyde To a composition for relieving hangover.

Hereinafter, the present invention will be described in detail.

The composition for relieving hangover of the present invention contains Angelica gigas extracts as an active ingredient.

The above refers to the root of perennial oriental herbaceous ginseng belonging to the subfamily of Ganoderma lucidum, which shows high anticancer effect on gastric cancer and lung cancer cells, and has a promoting effect of normal hepatocyte and also an inhibitory activity function. In addition, Angelica gangrene lowers the blood cholesterol level and lowers the triglyceride and total fat content of the liver, which is increased by alcohol administration, to prevent liver damage due to chronic alcohol consumption.

To prepare the Angelica giganta extract, Angelicae is first washed with water and dried.

Next, the washed Angelica gigas and the extraction solvent are mixed at a weight ratio of 1:10 to 30, preferably 1:15 to 20, at 50 to 100 ° C, preferably 60 to 90 ° C for 10 minutes to 10 hours , Preferably 2 to 8 hours, to prepare an extract.

The extraction solvent is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably water. The mixed solvent is not particularly limited, but methanol, ethanol, butanol or propanol of 20 to 80% is exemplified.

When the weight ratio of Angelicae gigantis extract to the extraction solvent is out of the above range, the active ingredient of Angelicae gigantis can be extracted in a small amount in the extract.

The thus-prepared Angelica gigantosa extract can be used as an extract itself or as powder for convenient use. In order to powder the Angelica gigantosae extract, the extract is concentrated under reduced pressure to reduce its volume, and then lyophilized by a freeze dryer to be powdered.

The composition containing the angelica guinea ginseng extract of the present invention as an active ingredient to inhibit the production of acetaldehyde and capable of resolving a good hangover is characterized in that the Angelica gigantosa extract is contained in an amount of 0.01 to 80% by weight, preferably 0.1 to 50% by weight .

The composition of the present invention can be formulated into various oral or parenteral administration forms.

Examples of formulations for oral administration include tablets, pills, hard, soft capsules, liquids, suspensions, emulsions, syrups, granules and the like. These formulations may contain, in addition to the active ingredient, a diluent such as lactose, dextrose, (For example, silica, talc, stearic acid and magnesium or calcium salts thereof and / or polyethylene glycol), in addition to the active ingredient (s). The tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and / or polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid Or a disintegrating or boiling mixture such as its sodium salt and / or an absorbent, a colorant, a flavoring agent and a sweetening agent. The formulations may be prepared by conventional mixing, granulating or coating methods.

Representative examples of formulations for parenteral administration include injectable preparations, and water, Ringer's solution, isotonic saline or suspensions may be mentioned as a solvent for the injectable preparation. The sterile, fixed oils of the injectable preparations may be used as a solvent or suspending medium, and any non-irritating fixed oils, including mono-, di-glycerides, may be used for this purpose. The injectable preparation may be a fatty acid such as oleic acid.

The dose of the hangover detoxification composition containing the Angelicae Radix extract of the present invention as an active ingredient varies depending on the age, sex, weight and severity of the disease of the patient, but the composition of the present invention is not more than 300 mg / Preferably, 10 to 200 mg is administered 1 to 4 times a day, but is not limited thereto.

The health functional food for hangover marine supernatant containing the Angelica keiskei Radix extract of the present invention as an active ingredient contains 0.01 to 80% by weight of Angelica gigantosa extracts based on the total weight of the health functional food.

The health functional food may be in the form of a capsule, tablet, powder, granule, liquid, ring, flaky, paste, syrup, gel, jelly and jute for the purpose of supplementing nutrients that may be lacking in daily eating or supplementing functional raw materials useful in the human body And is easily manufactured and operated once.

In addition, the health functional food may contain at least one selected from the group consisting of glucose, citric acid, liquid oligosaccharide, corn syrup, soybean lecithin, butter, vegetable hardening oil, skim milk, sugar, margarine, salt, starch, And can be manufactured by adding conventionally used components such as sodium bicarbonate and sugar ester, and additionally, for the purpose of preventing liver damage, by adding hematocrit (Hovenia dulcis) and / or silymarin (milk seed).

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Control group 1.

Water was used.

Example 1. Water extract of Angelica gigas Nakai

The roots of Angelica gigas Nakai were washed with water, dried and then mixed with 5 g of Angelica gigas Nakai and 100 ml of purified water. The mixture was covered with aluminum foil and hot water extracted at 80 ℃ for 8 hours.

Example 2. Ethanol extract of Angelica gigas Nakai

The same procedure as in Example 1 was carried out except that ethanol extract of Angelicae gigantis extract was prepared by extracting with 80% ethanol instead of water as an extraction solvent, concentrated under reduced pressure and adjusted to 100 ml with purified water.

Example 3. Extract of Angelica gigantea propanol

The same procedure as in Example 1 was carried out except that the extract was extracted with 80% propanol instead of water as an extraction solvent, and the resulting extract was concentrated under reduced pressure and adjusted to 100 ml with purified water.

<Test Example>

In the extracts prepared in Examples and Comparative Examples, when measuring the ADH inhibitory activity and ALDH activity, 0.1 ml of the diluted extract was added to the reaction solution after the dilution twice, and in the other experiment, the extract was used as it was.

Test Example 1. Measurement of Alcohol Dehydrogenase ( ADH) Inhibitory Activity

Preincubation of the test tube with distilled water, buffer (0.1 M phosphate buffer, pH 7.0), NAD ⁺ (6.25 mg / mL buffer soln.) And ADH (3 mg / mL buffer soln. Ethanol was added thereto, and the reaction was carried out at 30 ° C for 5 minutes. After completion of the reaction, the reaction mixture was cooled with ice water, and the absorbance at 340 nm was measured to determine the inhibitory activity (%) according to the following formula: Table 2] shows the inhibitory activity (%) relative to the control.

Conditions for measuring the inhibitory activity are shown in Table 1 below, and the results are shown in Table 2.

[Equation 1]

(%) = {1 - [(T-CC) - (T-TC) / C-CC]} x 100

T: Absorbance of the sample, TC: Absorbance of the sample control

C: Absorbance of enzyme reaction sphere, CC: Absorbance of sample and enzyme reaction control

division T TC C CC Distilled water (ml) 0.5 0.5 0.5 0.6 Buffer 0.5 0.6 0.6 0.6 NAD 0.15 0.15 0.15 0.15 ADH 0.1 - 0.1 - extract 0.10 0.10 - - Ethanol (99.9%) 0.15 0.15 0.15 0.15

division Control Example 1 Example 2 Example 3 ADH inhibitory activity (%) 0.0 67.0 65.5 64.6

As shown in Table 2 above, the extracts of Examples 1 to 3 of the present invention were confirmed to have high ADH inhibitory activity.

In particular, the water extract of Angelica gigas Nakai showed the highest ADH inhibitory activity, and the ethanol extract of Angelica gigantosa showed the higher ADH inhibitory activity.

Test Example  2. Aldehyde dehydrogenase ( ALDH ) Active measurement

To the test tube was added distilled water, buffer (1M-Tris-HCl, pH 8.0), 3M-KCl, 0.33M-2-Mercaptoethanol, NAD ⁺ (20 mM, 6.75 mg / ml in buffer) and ALDH (2.5 mg / ) And extracts were reacted at 25 ° C. for 5 minutes, and acetaldehyde (1 M) was added thereto. The reaction was carried out at 25 ° C. for 5 minutes. After completion of the reaction, the reaction mixture was cooled with ice water and then absorbance was measured at 340 nm The activity (%) was measured according to the following formula (2), and the activity (%) in [Table 4] indicates the relative activity (%) relative to the control.

The activity measurement conditions are shown in Table 3 below, and the results are shown in Table 4.

&Quot; (2) &quot;

(%) = {[(T-CC) - (T-TC) / C-CC]

T: Absorbance of the sample, TC: Absorbance of the sample control

C: Absorbance of enzyme reaction sphere, CC: Absorbance of sample and enzyme reaction control

division T TC C CC Distilled water (ml) 0.5 0.5 0.5 0.55 Buffer 0.5 0.55 0.55 0.55 3M-KCl 0.1 0.1 0.1 0.1 0.33M-2-mercaptoethanol 0.1 0.1 0.1 0.1 NAD 0.1 0.1 0.1 0.1 ALDH 0.05 - 0.05 - extract 0.05 0.05 - - Acetaldehyde 0.1 0.1 0.1 0.1

division Control Example 1 Example 2 Example 3 ALDH
activation(%) 100 112.3 105.5 100.8

As shown in Table 4 above, the extracts of Examples 1 and 2 of the present invention showed high ALDH activity.

In particular, the water extract of Angelica gigas Nakai showed the highest ADH inhibitory activity, followed by the ethanol extract of Angelica gigas Nakai.

Test Example  3. Evaluation of alcohol decomposition

In order to confirm the alcohol decomposing effect using the extracts prepared in the above Examples and Comparative Examples, the extracts were administered to the test animals together with the alcohol, and the blood alcohol concentration and acetaldehyde concentration after 1, 3 and 5 hours were measured, Was evaluated.

Six-week old SD rats were administered 3 mL of alcohol per kg of body weight, and the test product was administered twice 30 minutes before and after alcohol administration. Blood alcohol concentration and acetaldehyde concentration after 0, 1, 3, and 5 hr of alcohol administration were measured. The results are shown in Table 5 below.

division Blood alcohol concentration (% alcohol) Acetaldehyde concentration (mg / L) 1 hr 3 hr 5 hr 1 hr 3 hr 5 hr Control 0.032 0.034 0.028 8.6 6.7 5.5 Example 1 0.054 0.040 0.027 1.0 1.8 1.1 Example 2 0.053 0.038 0.025 1.3 1.7 1.2 Example 3 0.050 0.036 0.023 1.2 1.5 1.1

As shown in Table 5, it was confirmed that the alcohol content of the extracts of Examples 1 to 3 was higher than that of the control at 1 hour and 3 hours, although the blood alcohol concentration decreased with time. At 5 hours after administration, the alcohol concentration is slightly lower than that of the control group.

On the other hand, the extracts of Examples 1 to 3 confirmed that the concentration of acetaldehyde was remarkably reduced at all times compared to the control.

As a result, it is considered that the extracts of Examples 1 to 3 can protect liver and brain tissue by minimizing liver damage caused by acetaldehyde.

Test Example  4. Measurement of respiratory alcohol concentration

To each male and female of 20 to 25 years old, alcohol was administered at a concentration of 20 ° C / 20 ml / kg body weight in 1 minute, and 100 ml of the extracts of Examples and Comparative Examples were taken at 10, 60, And 120 minutes later, respiratory alcohol concentration was measured.

division Control Example 1 Example 2 Example 3 Breathing alcohol concentration (%) 10 minutes 0.015 0.024 0.022 0.023 60 minutes 0.018 0.030 0.027 0.026 120 minutes 0.007 0.009 0.010 0.010

 As shown in Table 6 above, respiratory alcohol concentration was decreased in all the groups until 60 minutes, but after 90 minutes, respiratory alcohol concentration began to decrease.

The extracts prepared according to Examples 1 to 3 of the present invention were found to have a higher respiratory alcohol concentration than the control.

That is, as a result of Test Examples 3 and 4, the extracts prepared according to Examples 1 to 3 showed a high alcohol content and a low acetaldehyde content.

Test Example  5. Stability measurement at pH 2

The extracts prepared in the above Examples and Comparative Examples were adjusted to pH 2 with 35% HCl, treated at 37 ° C for 0.5-4 hours (gastrointestinal condition) and adjusted to pH 7.0 with 36% NaOH Alcohol dehydrogenase (ADH) inhibitory activity was measured according to the method of Test Example 1, and the results are shown in Table 7 below. The inhibitory activity (%) in the following [Table 7] shows the relative inhibitory activity (%) against the control (0%).

division Example 1 Example 2 Example 3 ADH inhibition
activation(%) 0.5 hr 67.1 65.5 64.6 1 hr 67.3 65.0 64.4 2 hr 66.8 65.8 65.5 4 hr 66.5 65.2 64.4

As shown in Table 7 above, it was confirmed that the extracts of Examples 1 to 3 were stable at pH 2.0 with almost no change in ADH inhibitory activity when they were treated at pH 2.0 and 37 ° C, which are conditions in the stomach, for 4 hours.

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation example  One. Sanje  Produce

The extract powder obtained in Example 1                     500 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

Formulation example  2. Preparation of tablets

300 mg of the extract powder obtained in Example 1

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

Formulation example  3. Preparation of capsules

200 mg of the extract powder obtained in Example 1

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Formulation example  4. Preparation of injections

600 mg of the extract powder obtained in Example 1

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO _{4 ,} 12H ₂ O 26 mg

It is prepared by the above-mentioned component content per ampoule according to the usual injection preparation method.

Formulation example  5. Liquid  Produce

4 g of the extract powder obtained in Example 1

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100g by adding purified water, To prepare a liquid agent.

Formulation example  6. Preparation of granules

1,000 mg of the extract powder obtained in Example 1

Vitamin mixture quantity

70 ug of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2 ug

Vitamin C 10 mg

Biotin 10 ug

Nicotinic acid amide 1.7 mg

Folic acid 50 ug

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above-mentioned vitamins and minerals is comparatively comparatively mixed with the granules according to the preferred embodiment. However, the blending ratio may be arbitrarily changed, and the above components are mixed according to the ordinary granule preparation method, Can be prepared and used in the manufacture of a health functional food composition according to a conventional method.

Formulation example  7. Manufacture of functional beverages

1,000 mg of the extract powder obtained in Example 1

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added and the entire 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the compounding ratio may be arbitrarily varied depending on the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims (6)

A composition for relieving hangover characterized by containing Angelica ganoderma extract as an active ingredient. [3] The composition for eliminating hangover according to claim 1, wherein the Angelica keiskei koidz. Extract is prepared by mixing Angelica gigas Nakai, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof at a weight ratio of 1:10 to 30. The composition for removing hangover according to claim 1, wherein the mixed solvent is 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol. [3] The composition for removing hangover according to claim 2, wherein the extraction temperature is 50 to 100 &lt; 0 &gt; C. A health functional food for hangover resolution characterized by containing Angelica ganoderma extract as an active ingredient. The health functional food according to claim 5, wherein the health functional food is selected from the group consisting of capsules, tablets, powders, granules, liquids, rings, flakes, pastes, syrups, gels, .