KR102310813B1 - Composition for relieving hangover comprising matured silkworm and yeast extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for relieving hangover containing sleep apnea and yeast extract as active ingredients, and specifically, the complex of sleep sleep and yeast extract containing glutathione according to the present invention is an alcohol dehydrogenase (alcohol) involved in the alcohol decomposition process. It significantly improves dehydrogenase; ADH) and aldehyde dehydrogenase (ALDH) activity and rapidly decomposes alcohol and acetaldehyde in the body, so it can be usefully used as an active ingredient in a composition for preventing or relieving a hangover.

Description

Composition for relieving hangover comprising matured silkworm and yeast extract as an active ingredient

The present invention relates to a composition for relieving hangover containing hangover and yeast extract as active ingredients, and more particularly, to a composition for relieving hangover, which contains, as active ingredients, a hangover and yeast extract having an excellent effect in relieving a hangover after drinking. .

Drinking is becoming essential for modern people who have frequent dinner parties and entertainment and are stressed by various factors, and as a result, drinking opportunities are significantly increasing. According to the results of the National Health and Nutrition Examination Survey conducted by the Korea Institute for Health and Social Affairs under the Ministry of Health and Welfare recently, in Korea, adults aged 20 years and older drink alcohol on average about 8 days a month, about 11 days for men and about 4 days for women. At this time, the frequency of intoxication was found to be about 47% more than once a week, about 107% from 1 to 3 times a month, and about 132% from 1 to 3 times in 3 months. This is quite worrisome from this point of view.

As such, modern people consume an excessive amount of alcohol to an extent that the human body cannot afford it, and interest in drugs or beverages capable of removing a hangover is also steadily increasing. According to Nielsen Korea, a market research firm, the domestic hangover relief market sales amounted to only 60 billion won in 2005, but recorded 114 billion won in 2008, 205.8 billion won in 2011, 196.6 billion won in 2014, and 133.3 billion won in 2015. As such, the hangover relief market has grown to exceed the current sales of 100 billion won, and is attracting attention from overseas markets as well.

In the general alcohol metabolism process, when alcohol is consumed, it is mainly absorbed in the stomach or small intestine, and up to 1/3 of it is decomposed by alcohol dehydrogenase (ADH) in the stomach, depending on the person, to minimize the absorption of alcohol through the blood. The rest is transported to the liver and metabolized. About 90% of alcohol reaching the liver is oxidized to acetaldehyde by ADH and then oxidized to acetic acid by acetaldehyde dehydrogenase (ALDH). The remaining 10% of alcohol is metabolized to acetaldehyde by catalase. Acetaldehyde, which is the primary metabolite essential in the metabolic process of ingested alcohol, causes the biggest cause of acute alcohol hangover, namely nausea, vomiting, dizziness, thirst, lethargy, etc., leading to a decrease in work ability of the general public. In addition, if alcoholic fatty liver or alcoholic hepatitis occurs and continues to drink alcohol while the liver is in a bad state, cirrhosis may occur.

Therefore, studies of agents capable of removing a hangover caused by alcohol intake and reducing blood alcohol concentration have been actively conducted.

Herbal medicines mainly used for hangover remedies include sagebrush, radish, wormwood, arrowroot, persimmon leaf, sagebrush, sagebrush, nogeun, heartburn, gold and silver flower, upper leaf, goji berry, cheonmundong, longan meat, gardenia, tosaja, Haeseong tea, green tea, licorice, brown flower, lotus cheongpi, mokhyang, tangerine peel, ginseng, jeoryeong, baekbokryeong, shinguk, taeksa, dried ginger, baekchul, baekduguin, sine, oligosaccharide, gurume, etc. As a hangover remover, various drinks containing the above herbal medicines are currently on the market, and these drinks are consumed either alone after drinking or by adding them to alcoholic beverages with high alcohol content before drinking. However, drinks containing some herbal medicines take a long time to relieve hangover, cause general malaise, bloating, vomiting, or abdominal pain, and have various problems such as high cost by using expensive herbal medicines. have. Therefore, it is easy and efficient to secure raw materials, and there is still a need to develop a product capable of resolving alcohol decomposition and hangover within a short time.

Accordingly, the present inventors have made efforts to develop a hangover reliever with fast alcohol decomposition and excellent effect of relieving hangover after drinking. As a result, the complex of yeast extract containing sleep and glutathione has ADH and ALDH activity involved in the alcohol decomposition process. By significantly improving and confirming that the body alcohol and acetaldehyde are rapidly decomposed, the present invention was completed by revealing that it can be usefully used as an active ingredient in a composition for relieving a hangover.

Republic of Korea Patent No. 10-1068128 Republic of Korea Patent No. 10-0385344 Republic of Korea Patent Registration No. 10-1859166

An object of the present invention is to provide a composition for relieving a hangover having an excellent effect in relieving a hangover after drinking.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or relieving a hangover containing sleep nap and yeast extract as active ingredients.

In addition, the present invention provides a health functional food composition for preventing or relieving hangover containing sleep apnea and yeast extract as active ingredients.

In addition, the present invention provides a food composition for preventing or relieving hangover containing sleep nap and yeast extract as active ingredients.

The complex of yeast extract containing sukjam and glutathione according to the present invention significantly improves alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities involved in the alcohol decomposition process, Since it rapidly decomposes alcohol and acetaldehyde in the body, it can be usefully used as an active ingredient in a composition for relieving a hangover.

1 is a view confirming the effect of a complex of a yeast extract containing sleep and glutathione according to an embodiment of the present invention on aldehyde dehydrogenase (ALDH) activity.
Figure 2 is a view confirming the effect of the complex of sukjam and glutathione-containing yeast extract according to an embodiment of the present invention on alcohol dehydrogenase (ADH) activity.
3 is a diagram schematically illustrating a clinical test method using a complex of sleep and glutathione-containing yeast extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention provides a pharmaceutical composition for preventing or relieving a hangover, comprising a hangover and yeast extract as active ingredients.

In the present invention, the matured silkworm is a cooked silkworm, wherein the silkworm is a silkworm that stops eating mulberry and moves in search of a place to fix it due to the extreme growth of Maimak young (齡). In addition, the sleep sleep may be prepared by a method comprising the following steps, but is not limited thereto, and commercially available ones may be used without limitation:

1) boiling the silkworm in water or steaming it with steam to cook;

2) The step of drying the cooked silkworm of step 1).

In the present invention, the drying in step 2) may be reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, and specifically may be freeze drying, but is not limited thereto. In addition, after the step 2), a step of pulverizing may be performed.

In the present invention, the yeast extract preferably contains glutathione, and specifically, it is preferable to contain glutathione in an amount of 7 to 10 parts by weight based on 100 parts by weight of the yeast extract. In addition, the yeast extract may be commercialized and sold as a commercial product may be used.

In the present invention, the composition may contain sukjam: yeast extract in a weight ratio of 1: 0.5 to 2, more specifically, in a weight ratio of 1: 0.7 to 1.5, but is not limited thereto.

In a specific example of the present invention, the present inventors prepared a complex stock solution and tablets of yeast extract containing sukjam and glutathione.

In addition, the complex stock solution and purification significantly improve the alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities involved in the alcohol decomposition process, and rapidly decompose alcohol and acetaldehyde in the body. confirmed that

Therefore, since the yeast extract containing hangover sleep and glutathione of the present invention exhibits an excellent effect in relieving hangover after drinking, it can be usefully used as an active ingredient in a pharmaceutical composition for preventing or relieving hangover.

The pharmaceutical composition for preventing or relieving a hangover of the present invention may further contain a pharmaceutically or physiologically acceptable carrier. As the carrier, any carrier that is pharmaceutically or physiologically acceptable according to a method for preparing a functional composition known in the art to which the present invention belongs, for example, lactose, dextrose, sucrose, sorbitol, mannitol , xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate ate, talc, magnesium stearate, mineral oil, and the like. In addition, the pharmaceutical composition for preventing or relieving a hangover of the present invention may further contain a filler, an anti-aggregant, a lubricant, a wetting agent, a fragrance, an emulsifier or a preservative.

In addition, the pharmaceutical composition for preventing or relieving hangover of the present invention is a natural flavor such as plum flavor, lemon flavor, pineapple flavor or herb flavor, natural fruit juice, natural pigment such as chlorphyllin, fructose, honey, sugar alcohol, sugar It may further contain sweetening agents such as and/or acidifying agents such as citric acid and sodium citrate, or mixtures thereof.

The pharmaceutical composition for preventing or relieving hangover according to the present invention may be formulated by a method well known in the art to control the release rate of the active ingredient after administration (eg, rapid, sustained or delayed release). have. The formulation may be in the form of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders, and the like.

In addition, the pharmaceutical composition for preventing or relieving a hangover according to the present invention may be administered through various routes such as oral, transdermal, subcutaneous, intravenous or intramuscular, but it is most preferable to use an oral administration method.

A preferred dosage of the composition for relieving a hangover according to the present invention varies depending on the absorption of the active ingredient in the body, the age, sex, and severity of symptoms of the patient, but may be appropriately selected by those skilled in the art. However, for a desirable effect, in the case of oral administration, the composition of the present invention per 1 kg of body weight per day is generally administered to adults at 0.0001 to 1000 mg/kg, more specifically, 0.001 to 700 mg/kg per day. have. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

In addition, the present invention provides a health functional food composition for preventing or relieving hangover containing sleep apnea and yeast extract as active ingredients.

In addition, the present invention provides a food composition for preventing or relieving hangover containing sleep apnea and yeast extract as active ingredients.

In addition, the present invention provides a hangover reliever containing hangover sleep and yeast extract as active ingredients.

The sleep and yeast extract, and their mixing ratio is the same as the description of the pharmaceutical composition for preventing or relieving hangover containing the sleep and yeast extract as an active ingredient. Only the specific composition of the nutraceutical composition or food composition will be described.

On the other hand, the complex of sleep nap and glutathione-containing yeast extract according to the present invention significantly improves the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase involved in the alcohol decomposition process, and rapidly decomposes alcohol and acetaldehyde in the body, so it is a hangover reliever It can be usefully used as an active ingredient in health functional food compositions and food compositions, including.

As used herein, "health functional food" refers to food prepared by adding the active ingredient of the present invention to food materials such as beverages, teas, spices, gum, and confectionery, or as a tablet, encapsulation, powder, suspension, etc. It means that it brings a specific effect on health, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis.

The food composition according to the present invention includes all foods such as beverages, meat, chocolate, food, confectionery, pizza, ramen, other noodles, gum, ice cream, alcoholic beverages, and vitamin complex, including hangover relievers. Although it cannot be uniformly defined according to the type of food, it may be added in a range that does not impair the original taste of the food, and is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight relative to the target food. In addition, in the case of food in the form of granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 5 to 100% by weight.

Hereinafter, the present invention will be described in detail by way of Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

<Example 1> Preparation of complex of yeast extract containing sukjam and glutathione

In order to prepare a complex of sukjam and glutathione-containing yeast extract, sukjam powder was used in Jeonbukbu through the Korean Jam Society. In addition, the yeast extract powder containing glutathione was purchased from OHJUN Life Sciences Co., Ltd. (Japan) and used. The glutathione-containing yeast extract was prepared using Candida Utilis , and it was confirmed that it contained 8.9% glutathione.

<1-1> Preparation of stock solution

After dissolving the yeast extract containing sukjam and glutathione in 20 ml of water according to the composition shown in Table 1 below by sonication for one hour, it was filtered through a 0.45 μl filter to prepare a stock solution of the complex.

ingredient Example 1-1 Content (mg) a good night's sleep 500 Yeast Extract with Glutathione 500

<1-2> Preparation of tablets

After mixing the yeast extract containing sukjam and glutathione in the composition shown in Table 2 below, tablets were prepared by tableting according to a conventional manufacturing method of tablets.

In addition, after mixing with the composition shown in Table 2 below, tablets were prepared by tableting according to a conventional tablet manufacturing method, and this was used as a control agent for human application tests.

ingredient Example 1-2 control agent Hamrang (mg) Mixing ratio (%) Content (mg) Mixing ratio (%) a good night's sleep 500 31.8 0 0 Yeast Extract with Glutathione 500 31.8 0 0 crystalline cellulose 0 0 1000 63.6 fructooligosaccharide 572 36.4 572 36.4 Sum 1572 100 1572 100

<Comparative Examples 1 to 4>

A single stock solution of sukjam or glutathione-containing yeast extract was prepared and used as Comparative Examples (Comparative Examples 1 and 2). In addition, hutgae single undiluted solution, which is used as a main ingredient in hangover relieving drink, and sukjam and hutgae complex undiluted solution were prepared and used as comparative examples (Comparative Examples 3 and 4). Comparative Examples 1 to 4 were mixed with the composition shown in Table 3 below and prepared by the method described in Example <1-1>.

In this case, as a comparative example, Heotgae fruit extract powder purchased from Saerom F&B was used.

ingredient Content (mg) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 a good night's sleep 1000 - - 500 Yeast Extract with Glutathione - 1000 - - bullshit - - 1000 500

<Experimental Example 1> Confirmation of acetaldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) activity

The effect of the complex of sleep nap and glutathione-containing yeast extract on the activities of aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH), which are key enzymes for hangover relieving efficacy, was confirmed.

Specifically, ALDH activity was measured using the complex stock solution of sukjam and glutathione-containing yeast extract prepared in Example <1-1> and Comparative Examples 1 to 4. ALDH activity was measured using Aldehyde Dehydrogenase Activity Colorimetric Assay Kit (Catalog Number MAK082, USA) manufactured by Sigma-aldrich. That is, by carrying out according to the manufacturer's procedure using the kit, the rate at which NAD+ used as a coenzyme is converted in the process of ALDH generating acetate from acetaldyhyde as a substrate is reduced to 450 nm. The absorbance was measured in the wavelength region, and it was expressed as a relative activity (%) with respect to Comparative Example 1 (FIG. 1 and Table 4).

In addition, ADH activity was measured using the complex stock solution of the yeast extract containing sukjam and glutathione prepared in Example <1-1> and Comparative Examples 1 to 4. ADH activity was measured using Sigma-aldrich's Alcohol Dehydrogenase Activity Colorimetric Assay Kit (Catalog Number MAK053, USA). That is, by performing according to the manufacturer's procedure using the kit, the rate of generation of NADH generated when ADH reacts with ethanol as a substrate was measured by absorbance in the 450 nm wavelength region, and the relative activity (%) for Comparative Example 1 (Fig. 2 and Table 4).

In this case, distilled water was used as a control.

As a result, as shown in FIGS. 1, 2, and Table 1, the complex stock solution (Example 1-1) of the yeast extract containing sukjam and glutathione was the stock solution (Comparative Example 1) and the glutathione-containing yeast extract stock solution (Comparative Example 2) ), it was confirmed that the ALDH and ADH activities were improved compared to the stock solution (Comparative Example 3) and Sukjam and Huotgae complex (Comparative Example 4). In addition, it was confirmed that the ALDH activity was lower in the case of the sukjam and hutgae complex undiluted solution (Comparative Example 4) than the sukjam undiluted solution (Comparative Example 1).

sample ALDH activity (%) ADH activity (%) control 0 0 Comparative Example 1 100 100 Comparative Example 2 103 96 Comparative Example 3 62 98 Comparative Example 4 94 102 Example 1-1 130 142

From the above results, it can be seen that the combination of yeast extract containing sleep apnea and glutathione is an excellent combination for the effect of relieving hangover.

<Experimental Example 2> Confirmation of blood alcohol and acetaldehyde

A human application test was conducted to find out the hangover relieving effect of the complex of sleep sleep and glutathione-containing yeast extract.

Specifically, the human application test was designed as a double-blind, randomized, placebo-controlled, cross-design human application trial as shown in the schematic diagram of FIG. 3 . If a person who voluntarily signed the consent form for human application participates in this human application test, demographic survey, medical history survey, drug administration history survey, physical examination, vital signs, weight measurement, clinical pathology test, pregnancy test (only for women of childbearing age) If it meets the selection/exclusion criteria by carrying out the corresponding), the human body application subjects were registered through randomization. After clinical pathology and pregnancy test (only for women of childbearing age) were performed as screening tests at Visit 1, exclusion criteria were confirmed and randomization was made at Visit 2, CP group (Visit 2: Example 1-2), and visit 3: Control agent (Placebo)) or PC group (Visit 2: Control agent (Placebo)), Visit 3: The administration was performed according to the double-blind procedure after being assigned to one of the groups. Visit 3 after Bingmun 2 was conducted after washing-out for 7 days or more and 10 days or less.

Thirty people who meet the criteria for selection and exclusion of human subjects were secured, and randomly assigned to visit 2, divided into 15 CP group and 15 PC group, respectively, test food (Example 1-2) and control food (control). tablets) were taken. On visit 3, 15 people in the CP group took the control food (control agent), and 15 people in the PC group took the test food (Example 1-2).

In addition, for the usefulness evaluation, blood was collected before drinking and 0.5, 1, 2, 4, and 8 hours after drinking to obtain the subject's blood, and the blood alcohol concentration and blood acetaldehyde concentration were measured using the obtained blood. Blood alcohol concentration was measured through blood and exhalation tests. That is, blood alcohol and acetaldehyde concentrations were analyzed using gas chromatography mass spectrometry (GC-MS). Mass spectrometer (MASS) is a device that separates and records ions generated by applying energy to molecules according to their m/z (mass to charge) value. The amount required for analysis is several microgram-nanograms, so it is one of the most sensitive chemical analysis instruments. In the GC-MS method, a mass spectrum can be obtained for each compound in the mixture that can be analyzed by GC by separating the mixture from the GC column and introducing it to the mass spectrometer on-line. It is used for separation and qualitative analysis of organic compounds, quantitative analysis, and analysis of trace organic harmful components. In order to enable accurate measurement of blood alcohol and acetaldehyde obtained in this experiment, the analysis was requested by the Korea Functional Food Research Institute. It was delivered to the Functional Food Research Institute.

As a result, as shown in Tables 5 and 6, in the case of subjects who ingested a tablet containing as a main component a complex of sleep and glutathione-containing yeast extract, after drinking compared to blood alcohol and acetaldehyde concentrations 30 minutes after drinking After 2 hours, it was confirmed that the blood alcohol and acetaldehyde concentrations decreased significantly. In addition, it was confirmed that the blood alcohol and acetaldehyde concentrations were lower throughout the concentration measurement time compared to the subjects who took the control agent.

Average blood acetaldehyde concentration over time division n 0 hours of drinking 0.5 hours of drinking 1 hour of drinking 2 hours of drinking Example 1-2 intake 25 0.312 0.425 0.552 0.534 Contraceptive intake 25 0.362 0.587 0.589 0.682 p-value t-test 0.366 0.045 0.192 0.040

Average blood alcohol concentration over time division n 0 hours of drinking 0.5 hours of drinking 1 hour of drinking 2 hours of drinking Example 1-2 intake 25 24.71 1128.21 903.90 767.27 Contraceptive intake 25 13.36 1217.35 936.79 815.33 p-value t-test 0.438 0.224 0.621 0.264

Through the above results, it can be seen that the complex of sukjam and glutathione-containing yeast extract rapidly decomposes alcohol and acetaldehyde in the body.

Claims (10)

As a pharmaceutical composition for preventing or relieving hangover containing sleep nap and yeast extract as an active ingredient,
The composition is sukjam: yeast extract containing 1: 0.5 to 2 parts by weight, the yeast extract will contain glutathione (glutathione), the composition.
delete The pharmaceutical composition for preventing or relieving a hangover according to claim 1, wherein the glutathione is contained in an amount of 7 to 10 parts by weight based on 100 parts by weight of the yeast extract.
delete The pharmaceutical composition for preventing or relieving a hangover according to claim 1, wherein the composition promotes the decomposition of alcohol and acetaldehyde.
According to claim 1, wherein the composition is alcohol dehydrogenase (alcohol dehydrogenase; ADH) or acetaldehyde dehydrogenase (aldehyde dehydrogenase; ALDH) characterized in that increasing the activity, hangover prevention or relief pharmaceutical composition.
The composition of claim 1 , wherein the composition consists of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders. A pharmaceutical composition for preventing or relieving hangover, characterized in that it has a formulation selected from the group.
As a health functional food composition for preventing or relieving hangover containing sleep sleep and yeast extract as active ingredients,
The composition is sukjam: yeast extract containing 1: 0.5 to 2 parts by weight, the yeast extract will contain glutathione (glutathione), the composition.
As a food composition for preventing or relieving hangover containing sleep nap and yeast extract as active ingredients,
The composition is sukjam: yeast extract containing 1: 0.5 to 2 parts by weight, the yeast extract will contain glutathione (glutathione), the composition.
As a hangover reliever containing sleep sleep and yeast extract as active ingredients,
The hangover reliever is a hangover: a yeast extract containing 1: 0.5 to 2 parts by weight, the yeast extract containing glutathione (glutathione), a hangover reliever.

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