KR20170110976A

KR20170110976A - Method for Manufacturing Yeast powder coated by cocoon protein degradation material and Yeast powder coated by cocoon protein degradation material by the same

Info

Publication number: KR20170110976A
Application number: KR1020160035493A
Authority: KR
Inventors: 김상태
Original assignee: 주식회사 신도바이오실크
Priority date: 2016-03-24
Filing date: 2016-03-24
Publication date: 2017-10-12

Abstract

The present invention relates to a method for producing a yeast coated with silkworm protein decomposition product and a yeast coated with silkworm protein decomposition product produced by the method. The present invention optimizes the process of degrading cocoon protein and the process of coating cocoon protein decomposition product in yeast to produce a yeast having the best flavor and a unique yeast discomfort that the consumer has a sense of rejection. We have also developed a high - functional health supplement that combines yeast and silkworm proteolytic enzymes to improve nutritional balance and incorporate yeast and silkworm proteins into a single product.

Description

TECHNICAL FIELD The present invention relates to a method for producing a yeast powder coated with silkworm protein decomposition products and a yeast powder coated with silkworm protein degradation products,

The present invention relates to a method for producing a yeast powder coated with silkworm protein decomposition product and a yeast powder coated with the silkworm protein decomposition product produced by the above method. More specifically, a yeast powder is immersed in a silk amino acid solution hydrolyzed with silk protein of silkworm cocoons under a predetermined condition to form a certain thickness of amino acid (protein) layer on the surface of the yeast, thereby improving yeast protein nutrition balance and taste Powder and a method for producing the same.

Yeast is an excellent food rich in nutrients such as proteins, vitamins, nucleic acids, polysaccharides, minerals and enzymes. Of the nutrients that make up the yeast, 50% are proteins and contain large amounts of vitamin B and nucleic acids. Compared with the protein content of 100 g of food and 16 g of beef and 13 g of egg, the yeast having a protein content of 50% is a very good protein source and has excellent protein quality. The protein of yeast is especially suitable for supplementation of post-exercise protein which is high in content of leucine, isoleucine, and valine metabolized mainly in non-liver muscle, liver disease with weak digestive function of protein and muscle use.

In addition, yeast contains a large amount of ingredients that help maintain health, such as selenium (Se), which prevents oxidization of hepatocytes, and minerals and hydrolytic enzymes, such as chromium (Cr), which is effective for diabetes and stress relief . In addition, it is rich in potassium (K), phosphorus (P), magnesium (Mg), etc., and is good for hypertensive patients.

Yeast also helps to strengthen the anti-cancer action and immunity. It is due to the beta-glucan, a type of polysaccharide contained in yeast, which has antiviral, antibacterial, antifungal, antiparasitic and anti-cancer effects. Beta glucan is also effective in preventing adult diseases by lowering cholesterol, triglycerides, and low-density lipoprotein (LDL).

As described above, yeast contains many ingredients that are helpful for health, but also those who have a sense of rejection due to the unique flavor of the yeast. Consumers who want nutrition and efficacy of yeast but do not like the taste, consumers who consume yeast on yogurt or honey, or who have sensitive taste may choose other health supplements instead of yeast. In addition, if yeast protein and amino acid types are further increased, the yeast value as a nutrient can be further increased. Therefore, it is important to improve the nutrition and taste of yeast in order for yeast to become more popular as a health food.

On the other hand, the silk protein is a high-protein substance decomposing the cocoon. Silk, silk that forms the silkworm cocoon, is composed of sericin protein surrounding fibroin and fibroin, which is a collection of fibrils. When fibroin is hydrolyzed, it becomes free amino acid and silk peptide. A silk peptide is a substance in which two or more amino acids are bonded to each other and contains a total of 18 amino acids including eight essential amino acids. Silk peptides have recently become increasingly used as health supplements because of their medical effects such as lowering of blood cholesterol, elimination of hangover, prevention of senile dementia, and inhibition of non-insulin dependent diabetes mellitus. In addition, silk peptides (amino acids) are sweet and have good digestion and absorption and are being developed as various foods.

Specifically, in Japanese Patent Application Laid-open No. Hei 1-256352, a food using a silk protein as it is and a manufacturing method thereof have been introduced. The above-described technique has a limitation in being used for various foods because it is difficult to make the silk protein to be used at a high concentration and at the late stage of absorption when the silk protein is ingested by itself without treatment. Korean Patent No. 0075546 resolves silk proteins into low molecular weight amino acids in order to solve the problems of the Japanese patent, thereby facilitating digestion and absorption.

However, silk protein also has room for improvement in terms of nutrition and taste like the yeast, and new products are required in the market.

Japanese Laid-Open Patent Publication No. 1989-256352 (published October 12, 1989) Korean Patent No. 10-0075546 (published on May 20, 1993)

Choi, Hyoung-Sook, Lee Young-Soon, Hyun-Ok Lee, Mi-Ok Kim, Seo Hyun Kim "Advanced Nutrition Using the Keyword" "The Nutrient Encyclopedia" by Munhong Hong and Hong-Young Hee (SHUFU TO SEIKTSU SHA Co., Ltd., Tokyo, 2005) Oliver DK Maddocks, Celia R. Berkers, Susan M. Mason, Liang Zheng, Karen Blyth, Eyal Gottlieb & Karen H. Vousden, "Serine starvation inducing stress and p53-dependent metabolic remodeling in cancer cells", Nature 493, 542-546 (24 January 2013)

The present invention was made to solve the above problems, and the present invention was invented to enhance the nutritional composition of the yeast and improve the taste of the yeast.

It is an object of the present invention to provide a yeast powder coated with silk protein degradation products such as silk amino acids.

It is another object of the present invention to provide a method for coating a yeast with a silk protein degradation product such as a silk amino acid.

In order to accomplish the above-mentioned object, the present invention provides a method for producing a silkworm protein proteolytic coating yeast, comprising the steps of: (A) preparing a silkworm cocoon removing a pupa and a foreign substance; (B) mixing an aqueous acid solution with the cocoon cocoons and reacting them with each other to obtain an aqueous solution of the silkworm protein decomposition product by decomposing the cocoon cocoin protein; (C) neutralizing the aqueous solution of the silkworm protein decomposition product by adding a base or an aqueous base solution thereto; (D) 400 to 1000 parts by weight, preferably 500 to 900 parts by weight, more preferably 600 to 800 parts by weight, of yeast per 100 parts by weight of silkworm protein decomposition product, based on dry weight, of the neutralized silk protein hydrolyzate solution Mixing the silk protein hydrolyzate with the yeast; (E) lyophilizing the coated yeast; And (F) pulverizing the lyophilized yeast.

In addition, the method for producing a silkworm proteolytic protease yeast of the present invention may further comprise a step of desalting the aqueous solution of the neutralized silk protein hydrolyzate before the step (C) and after the step (D).

In addition, the method for producing a silkworm proteolytic protease yeast according to the present invention may further comprise the step of purifying the aqueous solution of the neutralized silk protein hydrolyzate before the step (C) and after the step (D).

Further, the method for producing a silkworm proteolipid coating yeast according to the present invention further comprises a step of centrifuging or vacuum-concentrating a mixture of the aqueous solution of the silk protein hydrolyzate and yeast prior to the step (D) and after the step (E) can do.

In addition, the method for producing a cocoon proteolytic coating yeast of the present invention may further include washing the yeast coated with the silkworm protein decomposition product before centrifugation or vacuum concentration and before freeze-drying.

In addition, the method for producing a silkworm proteolipid coating yeast of the present invention may further comprise a step of sterilizing a mixture of the aqueous solution of the silkworm protein protease and the yeast before the step (D) and after the step (E).

In addition, the sterilization may be performed at 134 to 145 캜 for 1 to 2 seconds.

Further, the acid aqueous solution of step (B) may be an aqueous hydrochloric acid solution.

The acid aqueous solution of the step (B) may be 80 to 150 parts by weight, preferably 90 to 130 parts by weight, more preferably 100 to 120 parts by weight, of an acid aqueous solution of 3 to 6 N per 100 parts by weight of the cocoon .

In addition, the base of step (C) may be sodium hydroxide.

In addition, the reaction of step (B) may be carried out for 40 to 80 hours, preferably 48 to 72 hours, more preferably 55 to 65 hours.

The reaction of step (B) may be carried out at 100 to 120 ° C, preferably at 100 to 110 ° C.

Further, the purification can be carried out using activated carbon, diatomaceous earth, or activated carbon and diatomaceous earth.

The yeast of step (D) may be prepared by mixing 100 parts by weight of yeast and 5 to 500 parts by weight of a water-soluble carbohydrate, preferably glucose, sugar, erythritol, xylitol, sorbitol, mannitol, May be granules or powder which is obtained by mixing parts by weight with water or alcohol and then evaporating the water or alcohol and then pulverizing.

In addition, the mixing of step (D) may be carried out in a mixer with an impeller, preferably a turbine or a downward propeller.

In addition, the scum may be removed by a scum removing device installed in the vicinity of the liquid level in the rotating shaft of the impeller of the step (D).

The mixing of step (D) may be performed in a mixer equipped with an ultrasonic generator.

In addition, the aqueous solution of the neutralized silk protein hydrolyzate of step (D) may be 10 to 25 Brix, preferably 13 to 20 Brix.

In addition, the coating of step (D) may be carried out for 6 to 15 hours, preferably 8 to 12 hours.

Further, the coating of step (D) may be carried out at 5 to 95 캜, preferably 10 to 90 캜, more preferably 30 to 50 캜.

On the other hand, the silkworm proteolytic activity coating yeast of the present invention is characterized by being produced by the above method.

On the other hand, the yeast coated with the silkworm protein decomposition product of the present invention is characterized in that part or all of the surface of the yeast is coated with the cocoon protein decomposition product.

The yeast coated with the silkworm protein decomposition product of the present invention is characterized in that the weight ratio of the silkworm protein decomposition product to the yeast is 400 to 1000 parts by weight, preferably 500 to 900 parts by weight per 100 parts by weight of the silkworm protein decomposition product, By weight, and more preferably 600 to 800 parts by weight.

In addition, the yeast coated with the silkworm protein decomposition product of the present invention may have a thickness of 0.25 to 1.2 탆, preferably 0.3 to 1.0 탆, of the silkworm protein decomposition product coating layer.

In addition, the yeast coated with the silkworm protein decomposition product of the present invention may contain 400 to 1000 parts by weight, preferably 450 to 900 parts by weight, more preferably 500 to 900 parts by weight of yeast per 100 parts by weight of the silkworm protein decomposition product, 800 parts by weight, and the silkworm protein decomposition product may be an amino acid, a peptide, or an amino acid and a peptide.

The yeast powder coated with the silkworm protein decomposition product prepared according to the method of the present invention is nutritionally excellent including the nutrients of the yeast itself and the amino acids of the silkworm cocoons, and can be used for preventing hypertension, improving liver disease, , The prevention of dementia, and the suppression of diabetes mellitus can provide both the efficacy of yeast and cocoon protein. In particular, the balance of glycine, alanine and serine is improved.

Glycine is a potent antioxidant of glutathione, and according to Earl L. Mindell's book "Vitamin Bibles New Vitamin Bible" (Lee, 1985) translated by Ryu Young-hoon, glycine helps to treat dysfunction of the pituitary gland, It is also effective for the treatment of progressive asthenia because it constitutes the essential creatine of It has been used to treat bipolar disorder and hyperactivity disorder as an essential ingredient for the proper functioning of the central nervous system, and to prevent epileptic seizures. Glycine promotes the secretion of glucagon (insulinotropic hormone) that causes glycogen to flow. Since glycogen is secreted into the blood in the form of glucose, many doctors use it to treat hypoglycemia. It is also effective in treating gastric hyperplasia, so it enters many antacids. It is also used for the treatment of pathological acidosis in which blood pH (pH) is lowered. Especially, it is effective for acidosis due to imbalance of leucine. Michelle L. Rose et al. (Michelle L. Rose, Russell C. Cattley, Corrie Dunn, Victoria Wong, Xiang Li, Ronald G. Thurman, "Dietary glycine prevents the development of liver tumors caused by the peroxisome proliferator WY- , Carcinogenesis (1999) 20 (11): 2075-2081) reported that ingestible glycine inhibited hepatoma cell proliferation by peroxisome proliferators WY-14, 643.

On the other hand, Philip Felig et al. (Philip Felig, Thomas Pozefsk, Errol Marlis, George F. Cahill, "Alanine: Key Role in Gluconeogenesis", Science 13 February 1970: Vol. 167 no. 3920 pp. 1003-1004) It has been reported that it is involved in the role of glucose in the hypoglycemic state or in the fasting state of 4 to 6 weeks in the liver.

Finally, Choline, synthesized from serine, is involved in the synthesis of acetylcholine, a neurotransmitter, according to Choi Hyang-suk, Lee Young-sun, Lee Hyun-ok, Kim Mi-ok, According to the "Encyclopedia of Nutrients" Translated by Hong Young-hee (SHUFU TO SEIKTSU SHA Co., Ltd. Tokyo, 2005), acetylcholine can prevent hypertension by dilating blood vessels and lowering blood pressure. And, choline is the material of lecithin that constitutes cell membrane, and lecithin prevents cirrhosis and atherosclerosis because it prevents cholesterol from attaching to blood vessel wall and fat accumulation in liver. In addition, choline helps memory formation in the brain and enhances memory. Oliver DK Maddocks et al., "Serine starvation induces stress and p53-dependent metabolic remodeling in cancer cells", Nature (ed.), Nature, Vol. 493, 542- 546 (24 January 2013) reported that when serine is deficient, stress induces stress-induced metastasis and that P53-dependent metabolic degeneration occurs in cancer cells.

In addition, by coating yeast with a sweet cocoon protein decomposition product, it is possible to alleviate the pungent taste and odor peculiar to the yeast, and improve the taste to be soft and sophisticated, thereby increasing consumer's preference.

1 is an enlarged photograph showing the surface of a dried yeast.
Fig. 2 is an enlarged photograph showing the surface of a yeast coated with silkworm protein decomposition product.
3 is an enlarged photograph showing a cross-section of a dried yeast observed by cutting with an ion beam.
Fig. 4 is an enlarged photograph showing a cross-section of a yeast coated with silkworm protein degradation product observed by cutting with an ion beam.
Fig. 5 is an enlarged photograph of the surface of the coating layer of the silkworm protein decomposition product where cracks occurred.
Fig. 6 is an enlarged photograph of the surface of the silkworm protein decomposition product partially separated from the coating layer.
Fig. 7 is a table comparing the free amino acid test results of the control group and Example 1 of the present invention. Fig.
8 is a table comparing the test results of the constituent amino acids of the control group and Example 1 of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. In the following description, numerous specific details, such as specific elements, are set forth in order to provide a thorough understanding of the present invention, and it is to be understood that the present invention may be practiced without these specific details, It will be obvious to those who have knowledge of. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

In order to accomplish the above-mentioned object, the present invention provides a method for producing a cocoon cocoons, comprising the steps of: (A) preparing a silkworm cocoons removing pupae and foreign matter; (B) mixing an aqueous acid solution with the cocoon cocoons and reacting them with each other to obtain an aqueous solution of the silkworm protein decomposition product by decomposing the cocoon cocoin protein; (C) neutralizing the aqueous solution of the silkworm protein decomposition product by adding a base or an aqueous base solution thereto; (D) mixing the neutralized silk protein hydrolyzate aqueous solution with yeast to coat the silk protein hydrolyzate with the yeast; (E) lyophilizing the coated yeast; And (F) pulverizing the lyophilized yeast. The present invention also provides a method for producing a cocoon protein degradation product coated yeast.

First, in step (A) of the method according to the present invention, a part of the cocoon is cut to remove the pupa inside. Gasoline, trichlorethylene, or other solvent is used to wash the oil of the cut silkworm cocoons, and the inorganic impurities are washed away using water. Sericin is removed with an aqueous solution containing hot water, a soap solution, or an enzyme.

In step (B) of the method according to the present invention, the cocoon piece of step (A) is added to 80 to 150 parts by weight, preferably 90 to 130 parts by weight, of an aqueous 3 to 6 N acid solution per 100 parts by weight of cocoon cocoons Preferably 100 to 120 parts by weight, are mixed and reacted with each other. The reaction of step (B) is maintained at 100 to 120 ° C, preferably at 100 to 110 ° C for 40 to 80 hours, preferably 48 to 72 hours, more preferably 55 to 65 hours.

When the amount of the aqueous acid solution to be mixed per 100 parts by weight of the silkworm cocoons, the reaction time and the reaction temperature are within the above range, the hydrolysis of the cocoons proceeds smoothly and the production of free amino acids and oligopeptides The ratio can be adjusted at a desired level.

The acid is not limited as long as it can decompose the protein of cocoon cocoons into amino acids. For example, hydrochloric acid, sulfuric acid, nitric acid and the like can be used, and hydrochloric acid is particularly preferable.

In step (C) of the method according to the present invention, a base or aqueous base solution is added to the aqueous solution of the cocoon proteolytic degradant of step (B) to neutralize it. The base is not limited as long as it can neutralize the aqueous solution of the acidic silkworm protein decomposition product. For example, sodium hydroxide, magnesium hydroxide, potassium hydroxide, calcium hydroxide and the like can be used, and sodium hydroxide is particularly preferable. After neutralization, the protein solution is decolorized using activated carbon, which is a food additive, and purified by adding activated charcoal, diatomaceous earth, activated charcoal, and diatomaceous earth to obtain a protein solution in a liquid phase. Then, the salt of the proteolytic aqueous solution is removed.

In step (D) of the method according to the present invention, the yeast is mixed with the aqueous solution of the hydrocracked cocoon protein solution to coat the cocoon protein decomposition product on the surface of the yeast. More specifically, it is preferable that 400 to 1000 parts by weight, preferably 450 to 900 parts by weight, more preferably 500 to 800 parts by weight of the yeast is used per 100 parts by weight of the silkworm protein decomposition product based on the dry weight, 10 to 25 Brix, preferably 13 to 20 Brix. The coating of step (D) is also carried out at a temperature of 5 to 95 DEG C, preferably 10 to 90 DEG C, more preferably 30 to 50 DEG C for 6 to 15 hours, preferably 8 to 12 hours.

In the step (D), it is important to control the weight ratio of the cocoon protein degradation product and the yeast, the concentration of the solid content, the reaction time, and the reaction temperature. As a result, the coating thickness and coating density of the coco peptic proteolytic material and the adhesion between the coating layer and the yeast are different, which affects the taste and nutrition of the product.

If the yeast is less than 400 parts by weight based on 100 parts by weight of the silkworm protein decomposition product on the basis of the dry weight, the coating layer of the silkworm protein decomposition product becomes excessively thick, or particles composed only of the silkworm protein decomposition product are produced without yeast coating , Whereas if it exceeds 1000 parts by weight, the silkworm protein decomposition product may not be coated or coated with the yeast, but the coating layer may be too thin to be easily peeled off.

As described above, an aqueous solution of the silkworm protein decomposition product of 10 to 25 Brix does not cause an excessively high viscosity, so that it is possible to ensure workability and form a silkworm protein decomposition product coating layer having an appropriate thickness.

In addition, the mixing time for the yeast coating is preferably 6 to 15 hours in view of the thickness of the coating layer and the economical efficiency.

Meanwhile, the yeast of step (D) is preferably well dispersed without being aggregated when added to the aqueous solution of silkworm protein decomposition product, and may be granules or powder mixed with water-soluble carbohydrate for improving dispersibility. Specifically, 5 to 500 parts by weight, preferably 10 to 300 parts by weight of a sugar selected from water-soluble carbohydrate, preferably glucose, sugar, erythritol, xylitol, sorbitol, mannitol and mixtures thereof, together with 100 parts by weight of yeast, After mixing, the water or alcohol may be evaporated and then granulated or powdered to increase the dispersibility.

In addition, the mixing of step (D) may be performed in a mixer equipped with an impeller so that the cocoon protein decomposition product can be coated well on the yeast. The impeller that can be used in the present invention can be used without restriction if it can provide mixing power, and particularly turbines or downward propellers are preferred. A yeast that can not be mixed with the aqueous solution may be accumulated in the rotating shaft of the impeller in the vicinity of the liquid surface. In order to smoothly mix the yeast with the cocoon proteolytic product, a scum removing device may be installed on the rotating shaft near the liquid surface.

Furthermore, mixing of the step (D) may be performed by an ultrasonic generator alone or in a mixer provided with the impeller for improving the dispersibility of the yeast.

After step (D) above, the mixture of the aqueous solution of the silk protein hydrocracker and the yeast is centrifuged or concentrated under reduced pressure and sterilized at 134 to 145 ° C for 1 to 2 seconds.

In addition, the method for producing a cocoon proteolytic protease yeast of the present invention may further include washing the yeast coated with the silkworm protein decomposition product before centrifugation or lyophilization after concentration under reduced pressure. This allows removal of uncoated cocoons proteolytic, neutralized salts, and excess water soluble carbohydrates. If such washing step is added, the washing step may be followed by centrifugation or vacuum concentration again.

In step (E) of the method according to the invention, the yeast coated with the centrifugation or vacuum-concentrated and sterilized cocoon protein degradation product is dried with a freeze dryer.

Nutrient destruction that can occur when applying other drying methods can be minimized by applying this lyophilization.

In step (F) of the method according to the present invention, the yeast coated with the lyophilized silk protein degradation product of step (E) is ground to a size of, for example, 30 to 60 mesh.

The yeast powder thus prepared may be ingested by the powder itself or may be tableted for chewing or granulated to facilitate ingestion.

In the present invention, the yeast coated with the cocoon protein decomposition product is completed through the above steps, and is sealed and packaged as a multi-side aseptic packaging material with air and moisture-proof sealed packing material.

The present invention also provides a yeast coated with the silkworm protein decomposition product produced by the above-described method, wherein the yeast has a thickness of 0.25 to 1.2 mu m, preferably 0.3 to 1.0 mu m, 400 to 1000 parts by weight, preferably 450 to 900 parts by weight, more preferably 500 to 800 parts by weight of yeast per 100 parts by weight of amino acid, peptide, amino acid and peptide as the silkworm protein decomposition product. As described above, the coating thickness is derived from the range of optimum taste and nutrition while considering workability and economical efficiency.

EXAMPLES Hereinafter, the present invention will be described in detail with reference to Examples. However, the following Examples are intended to illustrate the present invention and are not to be construed as limiting the scope of the present invention.

Example 1: Cocoon protein Degradation product Preparation of Coated Yeast

[Cocoon protein decomposition process]

A portion of the cocoon was cut, the pupa inside was removed, and the cocoon was washed with gasoline and water in that order. Then, the cocoon was removed with an aqueous solution of soap, and 5 N Of hydrochloric acid aqueous solution 110 g were added and mixed. The solution was heated to 105 < 0 > Lt; / RTI > and allowed to decompose the cocoon proteins. The aqueous solution of the silk protein hydrolyzate of the silkworm cocoons was neutralized with an aqueous solution of sodium hydroxide, and the neutralized solution was desalted and added with activated carbon, followed by decolorization and filtration by stirring and filtration to obtain an aqueous solution of silkworm protein decomposition product.

[Production process of yeast powder coated with silkworm protein decomposition product]

Yeast was added to the aqueous solution of the cococcus proteolytic degradation product of 17 Brix, and 100 g of dry yeast was added to 20 g of silkworm protein decomposition product based on the dry weight, and the mixture was kept at 45 캜 for 10 hours. The mixture of the aqueous solution of cocoon proteolytic enzyme and yeast was concentrated under reduced pressure at 110 DEG C for 48 hours and sterilized at 140 DEG C for 2 seconds. The vacuum-concentrated and sterilized coco peptibacter protein-coated yeast was lyophilized and pulverized.

Example 2: Dry yeast and cocoon in the coating process Decomposition Change in weight ratio

The procedure of Example 1 was repeated except that the mass of the yeast injected into the aqueous solution of the silkworm protein decomposition product of Example 1 was changed to 80 g.

Example 3: Dry yeast and cocoon coating process Decomposition Change in weight ratio

The procedure of Example 1 was repeated except that the mass of the yeast injected into the aqueous solution of the silkworm protein decomposition product of Example 1 was changed to 200 g.

Comparative Example 1: Dry yeast and cocoon coating process Decomposition Change in weight ratio

The procedure of Example 1 was repeated except that the mass of the yeast injected into the aqueous solution of the silkworm protein decomposition product of Example 1 was changed to 40 g.

Comparative Example 2: Dry yeast and cocoon in the coating process Decomposition Change in weight ratio

The procedure of Example 1 was repeated except that the mass of yeast injected into the aqueous solution of silkworm protein decomposition of Example 1 was changed to 400 g.

Experimental Example 1: Sensory evaluation

The yeasts prepared in Examples 1 to 3 and Comparative Example 1 and Comparative Example 2 were tested for 20 persons (10 males and 10 females) in total sensory evaluation according to the following scale, The strength was evaluated. The evaluated results were averaged.

- 5 points: very good (taste) / very weak (unpleasant)

- 4 points: Good (taste) / weak (unpleasant)

- 3 points: normal (taste) / normal (unpleasant odor)

- 2 points: Not good (taste) / Strong (unpleasant odor)

- 1 point: Not very good (taste) / Very strong (unpleasant odor)

The results of the sensory evaluation are shown in Table 1 below.

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Control group flavor 4.5 4.4 4.2 3.1 2.5 1.7 Unpleasant odor 4.3 4.1 3.9 3.0 1.1 0.9

The control group shows the result of sensory evaluation for the dry yeast itself not coated with the silkworm protein decomposition product. The evaluation group reported that the yeast of the present invention, coated with silkworm protein decomposition products, had a more sophisticated taste, a smooth aftertaste, and a much lower bitter taste characteristic of yeast. In particular, as shown in Table 1 above, Examples 1 to 3 prepared at the composition ratio of the present invention were evaluated as having better taste and less discomfort than the comparative example and the control group. It can be seen that the weight ratio of the protein hydrolyzate and the yeast is greatly influenced by minimizing the optimum taste and discomfort. This is because the thickness and quality of the coco peptic proteolytic coating layer are greatly influenced by the weight ratio.

Fig. 1 is an enlarged photograph showing the surface of dry yeast, and Fig. 2 is an enlarged photograph showing the surface of yeast coated with silkworm protein decomposition product. Comparing the two figures, it can be seen that the surface of the yeast of FIG. 2 coated with the silkworm protein decomposition product is generally made rougher by the coating layer, and the peeled part in FIG. 2 is a part of the coating layer.

Fig. 3 is an enlarged view showing a cross-section of a dried yeast observed by cutting with an ion beam, and Fig. 4 is an enlarged photograph showing a cross section of a yeast coated with a silkworm protein decomposition product observed by cutting with an ion beam. When the two figures are compared, a cloudy region (indicated by a yellow dotted line) which can not be seen in FIG. 3 is observed at the upper part of the cross section of FIG. 4, which corresponds to a coating layer composed of cocoon protein decomposition products.

Fig. 5 is an enlarged photograph of the surface of the coating layer of the silkworm protein decomposition product, showing cracks, and Fig. 6 is an enlarged photograph of the surface of the coating layer of the silkworm protein decomposition product partially removed. In FIG. 5, it can be seen that cracks were formed on various surfaces. These cracks, which were not observed in FIG. 1, can be confirmed to be a coating layer of the cocoon protein decomposition product, not the surface of the yeast itself. Further, the area indicated by the red dotted line in FIG. 6 indicates that a part of the coating layer is peeled off, and the peeled surface is also rough, which indicates that the coating layer is not completely removed but peeling occurs in the middle of the coating layer.

FIG. 7 is a comparison of free amino acid test results (Korea Functional Food Research Institute) with the control group and Example 1, showing a 16% increase in crude protein as well as an increase in amino acid by 324% due to the coating of the silkworm protein decomposition product . This is a result of remarkably elevating the utility of the present invention in terms of nutritional supply considering that the amino acid absorption rate is about 3 to 10 times higher than that of protein. In particular, glycine was significantly increased by 14774%, alanine by 418%, serine by 1543%, and aspartic acid and threonine by 300% or more.

FIG. 8 is a graph comparing the results of the compositional amino acid test (KFRI) with the control group and Example 1, wherein glycine, alanine, and serine, which were prominent in FIG. 7, were 277%, 123%, and 65% As shown in Fig. As a result, the yeast coated with the silkworm protein decomposition product of the present invention has an advantage of being able to supply nutrients such as amino acids or proteins more effectively to the food-borne person by having the improved amino acid profile reinforced with the three amino acids as described above.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Accordingly, the scope of the present invention should not be construed as being limited to the above-described embodiments, but should be determined by equivalents to the appended claims, as well as the following claims.

Claims

(A) preparing a silkworm cocoon removing a pupa and a foreign substance;
(B) mixing an aqueous acid solution with the cocoon cocoons and reacting them with each other to obtain an aqueous solution of the silkworm protein decomposition product by decomposing the cocoon cocoin protein;
(C) neutralizing the aqueous solution of the silkworm protein decomposition product by adding a base or an aqueous base solution thereto;
(D) mixing 400 to 1000 parts by weight of yeast with 100 parts by weight of silkworm protein decomposition product based on the dry weight of the neutralized silk protein hydrolyzate aqueous solution to coat the silk protein hydrolyzate with the yeast;
(E) lyophilizing the coated yeast; And
(F) powdering the lyophilized yeast.

The method according to claim 1,
Before the step (C) and after the step (D)
Further comprising the step of desalting the neutralized aqueous solution of silkworm protein decomposition product.

The method according to claim 1,
Before the step (C) and after the step (D)
Further comprising the step of purifying the neutralized aqueous solution of silkworm protein decomposition product.

The method according to claim 1,
Wherein the acid aqueous solution of step (B) is an aqueous hydrochloric acid solution.

The method according to claim 1,
Wherein the aqueous acid solution of step (B) is 80 to 150 parts by weight of an aqueous acid solution of 3 to 6 N per 100 parts by weight of the cocoon of step (A).

The method according to claim 1,
Wherein the reaction of step (B) is carried out for 40 to 80 hours.

The method according to claim 1,
Wherein the reaction of step (B) is carried out at 100-120 < 0 > C.

The method of claim 3,
Wherein said purification is carried out using activated carbon, diatomaceous earth, or activated carbon and diatomaceous earth.

The method according to claim 1,
Wherein the neutralized aqueous solution of the silk protein degradation product of step (D) is 10-25 Brix.

The method according to claim 1,
Wherein the coating of step (D) is carried out for 6 to 15 hours.

A cocoon-coated proteolytic enzyme-coated yeast produced by the method of any one of claims 1 to 10.

A yeast coated with a silkworm protein decomposition product characterized in that a part or all of the yeast surface is coated with a cocoon protein decomposition product.

The method of claim 12,
Wherein the weight ratio of the silkworm protein decomposition product to the yeast is 400-1000 parts by weight per 100 parts by weight of the silkworm protein decomposition product based on the dry weight.

The method of claim 12,
Wherein the coating layer of the silkworm protein decomposition product coating layer has a thickness of 0.25 to 1.2 占 퐉.