KR20170091240A

KR20170091240A - Composition for antioxidant and whitening effects comprising extract of Tilia taquetii, Picrasma quassioides, Quercus mongolica, Geranium nepalense subsp. thunbergii

Info

Publication number: KR20170091240A
Application number: KR1020160011810A
Authority: KR
Inventors: 남궁우; 황혜민; 정소연; 이예진; 최누리; 황소담; 이득희; 유연호
Original assignee: 순천향대학교 산학협력단
Priority date: 2016-01-29
Filing date: 2016-01-29
Publication date: 2017-08-09
Also published as: KR101862093B1

Abstract

The composition according to one embodiment of the present invention contains an extract of a mixture containing mulberry leaf, tree, quince tree, and alopecia, and is widely used as a cosmetic composition or a food composition. The composition has excellent antioxidative activity, and is particularly excellent in whitening activity.

Description

A composition having an antioxidant and whitening complex functionalities, which comprises an extract of Mulberry Leaf Pinus densiflora, Pinus densiflora, a Quercus mongolica var. thunbergii}

The present invention relates to a composition containing an extract of a mixture comprising mulberry leaf, tree saplings, Quercus mongolica, and algae, and is useful as a cosmetic composition or a food composition and has a composite function of whitening and antioxidant activity will be.

Most Asian people have a desire for clean and fine skin, ie, whitening, for young and old whitening, compared to other continents. The skin color of a person largely depends on the amount of melanin synthesized mainly in pigment cells (melanocytes) existing in the basal layer of the skin.

Melanin pigment is most affected by genetic factors but also by various environmental and physiological factors such as stress and ultraviolet rays.

People with a lot of melanin pigments have brown or black skin, while those with very low melanin pigment cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in the melanocyte, a pigment cell present in the basement membrane of the skin.

Melanin pigment protects skin from excessive ultraviolet rays, and it plays an important role in suppressing skin damage and skin cancer caused by light rays. However, when melanin is excessively produced by daylight, hormone changes, inflammation, medicines, etc., the pigment of the skin is deposited, which causes various cosmetic problems. For example, skin pigmentation caused by hyperpigmentation of the skin is due to an increase in the amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased. Accordingly, in recent years, whitening cosmetics and pharmaceuticals have been developed and widely marketed in order to prevent and improve such problems.

Tyrosinase is an enzyme that acts as a catalyst in the oxidation of monophenols and is contained in the melanocytes of higher animals. Tyrosine is oxidized by this enzyme and melanin pigment is produced through a series of chemical reactions and oxidation.

In recent years, there have been developed and marketed a whitening agent which focuses on inhibiting the activity of tyrosinase, which is an enzyme that forms melanin pigment, and a method of decolorizing by reducing the already generated melanin pigment.

The tyrosinase activity inhibitors that have been studied so far include arbutin, alpha hydroxy acid (AHA), vitamin A, vitamin C, cysteine, azelaic acid, kojic acid, licorice extract, Mugwort extract, mexi extract, licorice extract, mulberry bark extract, and animal-derived placenta extract.

However, the whitening agent using tocopherol, vitamins or the like used for reducing the melanin pigment has an insufficient decolorizing effect of the melanin pigment, and therefore, an inhibitor that inhibits the formation of the melanin pigment by attracting the activity of the tyrosinase is attracting attention have.

Not only that, many women want to keep the skin that starts to naturally aging as a tough skin.

Humans experience two biological changes during their lifetime: growth and aging.

Growth means that the size or function of an individual increases, and aging means that the size or function of an individual decreases or declines. Growth and aging can occur at the same time, but aging phenomenon appears more prominently as time and age increase. Cell division and metabolic activity gradually weaken, do.

There are many hypotheses about the mechanism of aging.

In addition, it has been shown that a number of genes are involved in the cell cycle, such as the somatic mutation that causes aging due to the mutation of the gene, an error in the accumulation of abnormally produced proteins due to errors in the synthesis of the protein, There are various theories related to aging such as crosslinking theory, fiber theory, and free radical theory that aging progresses as crosslinking increases, but it is not yet clearly defined as the cause of aging.

However, free radicals are currently the most powerful. The theory is that free radicals act on cells and connective tissues to produce harmful substances, which accumulate and progress through aging.

Skin damage is caused by external factors such as various UV and radiation and internal stress factors. Among the main factors inducing such skin damage are very oxidative substances such as free radicals. These oxidative high-molecular substances, inducing factors damage the stratum corneum and the lipid layer, and drastically increase the transdermal water loss, resulting in skin dryness.

Antioxidation is the prevention of various oxidizations that occur in the body. Human aging is caused by O ₂ ^- (superoxide), NO (nitric oxide), NO ₂ (nitrogen dioxide), OH And hydroperoxyl radicals such as HO ₂ (hydroperoxyl) radicals. In vivo, it is known that superoxide dismutase (SOD), catalase (CAT), glutathione Antioxidant enzymes such as glutathione peroxidase (GSHpx), vitamin E and glutathione are present to protect the living body from oxidative damage by removing free radicals.

When these antioxidant defense systems are degraded to such an extent that free radical formation can not be controlled, the oxidative stress and damage of the tissues are promoted, and excess free radicals and lipid peroxides produced in the body are oxidized by oxidative stress such as protein oxidation and DNA damage Many synthetic / natural antioxidants have been developed for the reduction of oxidative stress. However, since synthetic antioxidants have safety problems, they are safe and have antioxidative effects using plant extracts such as various edible plant extracts. We are making efforts to develop excellent natural antioxidants.

In the conventional cosmetics field, as a result of active research on natural plant extracts or natural substances as a natural whitening extract, suppression of oxidative action inhibits reduction of elasticity due to aging and inhibits formation of melanin, A variety of methods for suppressing spot formation are sought.

Korean Patent Publication No. 10-2009-0124930 (published on June 22, 2011) Korean Published Patent Application No. 10-2012-0097091 (Publication date 2014.03.12.) Korean Patent Publication No. 10-1997-0014958 (published November 16, 1998).

It is an object of the present invention to provide a composition containing an extract of a mixture comprising mulberry leaf tree, alder tree, Quercus mongolica, and alopecia; and a multifunctional plant extract having both high antioxidant and whitening function, .

In order to achieve the above object, the cosmetic composition according to an embodiment of the present invention contains an extract of a mixture including mulberry leaf, tree, quince tree, and alfalfa.

The mixture may contain mulberry leaf, tree, mongolian and algae at a weight ratio of 2: 1.5 ~ 2.5: 0.5 ~ 1.5: 0.5 ~ 1.5, respectively.

The mixture may contain mulberry leaf, tree, mongolian and algae at a weight ratio of 2: 1.8-2.2: 0.8-1.2: 0.8-1.2, respectively.

The mulberry leaf of the mixture may be a leaf, a stem, or a mixture thereof. The mulberry tree contained in the mixture may be a leaf, a stem, or a mixture thereof. The mulberry tree contained in the mixture may be leaf, Stems, and mixtures thereof may be applied, and the spores contained in the mixture may be outposts.

The extract may be a water or alcohol extract of the mixture.

The extract may be a hot-water extract, a methanol extract, an ethanol extract or a spirit extract of the mixture.

The extract may have an antioxidative activity. The extract may be one having antioxidative activity by DPPH scavenging activity.

The extract may be one having whitening activity. The extract may be one having whitening activity by tyrosinase inhibition.

The extract may have a combined functionality of whitening activity and antioxidant activity.

The extract may be contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition.

The extract may be contained in an amount of 3 to 27% by weight based on the total weight of the cosmetic composition, and may be contained in an amount of 3 to 25% by weight.

The cosmetic composition further comprises at least one auxiliary ingredient selected from the group comprising lyserin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .

The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, Or more in cosmetics having the above-mentioned formulations.

A food composition according to another embodiment of the present invention contains an extract of a mixture comprising mulberry leaf, tree, mongolian, and alfalfa.

The extract may be a water or alcohol extract of the mixture.

The extract may be contained in an amount of 0.0001 to 30% by weight based on the total weight of the food composition.

The extract may be contained in an amount of 3 to 27% by weight based on the total weight of the food composition, and may be contained in an amount of 3 to 25% by weight.

Hereinafter, the present invention will be described in more detail.

The composition according to an embodiment of the present invention contains an extract of a mixture comprising mulberry leaf, tree, manganese, and alfalfa.

The mulberry blooming tree ( Tilia taquetii ) is a deciduous broad-leaved arboreous tree of the dicotyledonous plant (Tiliaceae) . It is also known as mulberry tree, and it is distributed in Gangwon, Pyongbuk, Chungbuk, Jeonnam, Jeonbuk, Jeju and so on. The young branches are dense with brown hairs, and the height is 5m. Leaves are alternate phyllotaxis, the tip is suddenly lengthened and pointed, and the bottom is under the heart. There is a sawtooth on the edge of the leaf, and the back vein (vein axilla) is dense with brown hairs. The petiole is 7 ~ 45mm long and has no hairs or brown hairs. The flowers bloom in June, bred in the mountain breeze, and run on the inflorescence. The bract is 2 to 3 mm in length, and the back has sparse hairs. The peduncle is 4-5mm long, with no hairs, and 3-5 flowers. The fruit looks like a long egg upside down. It has short hairs and ripens in October. Bark is used as a substitute for children, and used to make wooden bowls.

The picrasma quassioides) is galip keunki trees belonging to simaroubaceae (Simaroubaceae) sotae hawthorn. There are about 150 genera in the tropics and about 28 genera in the tropics. There are about 8 species in the world, and one species is distributed in our country. The trees grow up to a height of 9 to 12 meters. When it grows to some extent, the branch spreads in all directions and the top becomes rounded. It lives mainly in the sloping valley of 100 ~ 1100m high mountain, and the tattered rocky area of the middle. The bark becomes black gray-blue, but gradually becomes reddish-brown. The darker it is, the darker the dark brown color. It is flat and does not split very well, but it is rough with lots of skin. However, the nerves split vertically. The young branch is dark green, has no hair, and has distinctive yellowish-skinned eyes. The length of the small leaf is 4 ~ 10cm, width is 2 ~ 4cm, the end is long and pointed, the bottom is wide wedge, both sides are asymmetric, and there are sawtooths on the edge. Petiole is very short or very little. The front side of the small leaf has no hairs, it is glossy and there is no hair on the leaf vein on the back side. Calyx is 4-5, ovate, sometimes covered with thin hairs. The petals are 4-5, attached to the bottom of 4- calyx calyx. Flowering period is from April to June, and fertility period is from August to September. The egg-shaped nucleus and the fruit are black and ripe with calyx.

Trees are mostly wet and grow in fertile valleys, forests, and creeks. The name of the tree is called "gutae" because the bark of this tree is used like cattle. It contains a very bitter quassin, yellow bark on the bark, and a height of about 20 meters. The leaf is the right middle leaf, the small leaf is ovate, the bottom is round and the tip is sharp. The flower is yellowish-green, migrating in the form of acanthopteran in May-June, and the fruit is ovate, oval-shaped, ripened in red around September.

The Oak Wood (Quercus mongolica) is a deciduous tall tree that belongs to the dicotyledon Fagales Fagaceae Fagaceae (Fagaceae) native to the country and all over the country, especially in northern sanjidae. It is also distributed in China, Sakhalin, and Mongolia. It is also known as a scapegoat tree or pine tree. It grows mainly in mountainous areas, and it makes green leaves in high mountains. It is about 30m in height and about 1m in diameter. The bark is blackish brown and is divided vertically, and the winter snow is oval. There are no hairs on the whole, but the hybrids are hairy. The leaves are similar to oak trees but differ in that they do not have brown hairs on the back. It is a single male and female flower with April to May leaves. It is yellowish green. It is bloomed from the side of the leaf of the three base leaves. It grows straight from the upper part. Fruit is nuts ripened in September, length is 6 ~ 25mm, diameter is 6 ~ 20mm. The forbidden ponytail is bent and the back protrudes, and when the acorn falls, it shoots soon. Because wood is solid and patterned, it is widely used as sewing machine, high-quality furniture, plywood, and single-plate, and is also widely used as wood for building materials, sleepers and mushrooms.

The cranesbill (Geranium nepalense subsp . thunbergii ) is a mussel and perennial plant. It is about 50cm in height, and its roots have no straight roots and are divided into several pieces. The stem comes out and grows diagonally and spreads hair. Leaves run in opposite directions, split into 3 ~ 5 pieces, 3 ~ 7cm wide, and often have black pattern. The cut pieces are shaped like inverted eggs. The ends are dull and shallowly divided into three, with irregular sawtooth on the upper part. The petiole is long facing. Flowers bloom in June to August in light red, red purple or white with a diameter of 1-1.5cm. Flower stems come out from the axilla, and two small flower stalks are separated from flower stems and run one each. Short hairs and glandular hair on calyxes of small flower stems and calyxes, hairy on ovaries. The fruit is a capsule with a length of 1 ~ 1.2cm, divided into 5 pieces and dried back.

The inventors of the present invention have found that the extracts of each of these plants exhibit antioxidant activity or whitening activity, but the extract of these mixtures shows a superior effect to the dose in comparison with each of them, And particularly has excellent tyrosinase inhibitory activity.

The mixture may contain mulberry leaf, tree, mongolian and algae at a weight ratio of 2: 1.5 ~ 2.5: 0.5 ~ 1.5: 0.5 ~ 1.5, respectively. When a mixture containing these plants is used in such a weight ratio range, a composition having antioxidative activity and whitening activity at the same time can be prepared.

Preferably, the mixture may comprise a weight ratio of 2: 1.8 ~ 2.2: 0.8 ~ 1.2: 0.8 ~ 1.2, respectively, and most preferably 2: 1.9 ~ 2.1: 0.9 ~ 1.1: 0.9 to 1.1.

The mulberry leaf of the mixture may be a leaf, a stem, or a mixture thereof. The mulberry tree contained in the mixture may be a leaf, a stem, or a mixture thereof. The mulberry tree contained in the mixture may be leaf, Stems, and mixtures thereof can be applied, and outlines contained in the mixture can be applied to the outposts.

The extract may be a water or alcohol extract of the mixture. Here, the alcohol may be an alcohol having 1 to 5 carbon atoms.

The extract may have a complex function of a whitening activity and an antioxidative activity, and thus its use is particularly excellent when it is used as a cosmetic composition.

The extract may be contained in an amount of 3 to 27% by weight based on the total weight of the cosmetic composition, and may be contained in an amount of 3 to 25% by weight. When it is included in such a range, it may have whitening activity with antioxidative activity, and in particular, be equivalent to arbutin, which is a commercially available whitening active substance, or have a whitening activity better than arbutin. In the case of the above-mentioned extract, there is an advantage that it can be safely utilized because it is a non-toxic or small toxic substance derived from a natural plant.

The extract can be obtained by immersing the mixture which is a dried plant material in an extraction solvent, preferably by heating at 40 to 100 ° C for 2 to 10 hours.

The extraction solvent may be water, an organic solvent or a mixture thereof. The organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, butyl run- Can be applied.

The extract may be applied at a volume ratio of 1 to 10 based on the volume of the dried plant pulverized product, and the extract may be repeatedly extracted 1 to 5 times. The thus-prepared extract can be applied as an extract in powder form by removing the solvent by a method such as concentration under reduced pressure.

The composition may be applied as a pharmaceutical composition, a food composition or a cosmetic composition.

The pharmaceutical composition may comprise a pharmaceutically acceptable carrier or excipient. In the present invention, the expression "pharmaceutically acceptable" means that the application (subject) does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient. The "carrier" is defined as a compound that facilitates the addition of a compound into a cell or tissue.

The extracts of the present invention may be administered alone or in admixture with any convenient carrier, etc., and such dosage forms may be single-dose or repeated-dose formulations. The pharmaceutical composition containing the extract may be a solid preparation or a liquid preparation. Solid preparations include, but are not limited to, powders, granules, tablets, capsules, suppositories, and the like. Solid form preparations may include, but are not limited to, carriers, flavoring agents, binders, preservatives, disintegrants, lubricants, fillers, and the like. Examples of the liquid preparation include water, a solution such as a solution of propylene glycol, a suspension, an emulsion, and the like, but not limited thereto, and it can be prepared by adding a suitable coloring agent, a flavoring agent, a stabilizer, a tackifying agent and the like. For example, powders can be prepared by simple mixing of an extract, which is an effective ingredient of the present invention, with a suitable pharmaceutically acceptable carrier such as lactose, starch, microcrystalline cellulose and the like. The granule is the above-mentioned extract of the present invention; A suitable pharmaceutically acceptable carrier; And a suitable pharmaceutically acceptable binder such as polyvinylpyrrolidone and hydroxypropylcellulose, followed by wet granulation using a solvent such as water, ethanol, or isopropanol, or dry granulation using a compressive force . Tablets may also be prepared by mixing the granules with a suitable pharmaceutically acceptable lubricant such as magnesium stearate and then tableting using a tableting machine.

The extract of the present invention may be administered orally or parenterally depending on the disease to be treated and the condition of the subject, an injection (for example, intramuscular injection, intraperitoneal injection, intravenous injection, infusion, subcutaneous injection, implant) Rectal, rectal, transdermal, topical, and the like, but the present invention is not limited thereto. May be formulated into suitable dosage unit formulations, including those conventionally used and non-toxic, pharmaceutically acceptable carriers, additives, vehicles according to the route of administration.

The pharmaceutical composition of the present invention may be administered at a daily dose of about 0.0001 mg / kg to about 10 g / kg, with a daily dosage of about 0.001 mg / kg to about 1 g / kg being preferred. However, the dosage may vary depending on the degree of purification of the mixture, the condition of the patient (age, sex, weight, etc.), severity of the condition being treated, and the like. For convenience, the total daily dose may be divided as needed and divided into several doses throughout the day.

When the composition of the present invention is used as a pharmaceutical composition, the content of the extract in the composition can be appropriately adjusted to an effective amount capable of exhibiting anti-inflammatory activity according to the symptoms of the disease, the progress of symptoms, the condition of the patient, The amount may be 0.0001 wt% or more, specifically 0.001 wt% or more, and 80 wt% or less, specifically 50 wt% or less, based on the total weight of the composition, but is not limited thereto.

In the present invention, the composition may be a cosmetic composition.

The cosmetic composition can be prepared in various forms including, for example, emulsion, lotion, cream (underwater type, water type, multiphase), solution, suspension (anhydrous and aqueous), anhydrous product Gel, mask, pack, or powder. The cosmetic composition of the present invention may contain an acceptable carrier in cosmetic formulations in addition to its effective ingredient. As used herein, the term " acceptable carrier for a cosmetic preparation "means a compound or composition that is already known and used in the cosmetic preparation, or a compound or composition to be developed in the future, and which is toxic to the human body. The carrier may be included in the composition of the present invention in an amount of from about 1% by weight to about 99.99% by weight, specifically from about 50% by weight to about 99% by weight of the composition, based on the total weight thereof.

However, since the ratio depends on the above-mentioned formulation of the cosmetic composition and its specific application site (face or hands) or its preferable application amount, the ratio is limited in any aspect to the scope of the present invention It should not be.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, humectants, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents and perfumes. The compounds / compositions which can be used as the carrier and which can be used as alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosifiers, emulsions, stabilizers, sunscreens, A person skilled in the art can select and use appropriate substances / compositions.

Specifically, the cosmetic composition can be manufactured to have a formulation selected from a solution suspension, an emulsion, a gel, a cream, a lotion, a powder, a soap, a cleansing, a body wash, a cleansing and a spray, although the formulation is not limited.

In the present invention, the composition may be a food composition.

When the composition of the present invention is used as a food composition, it may contain an acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of food.

In the present invention, the term " food " means a natural product or a processed product containing one or more nutrients. Specifically, the term " food " means that the food can be directly eaten through a certain degree of processing. Functional foods, beverages, food additives, and beverage additives. Examples of the food include various foods, beverages, gums, tea, vitamin complex, and functional foods. In addition, the food of the present invention may contain special nutritional foods (eg, crude oil, spirit, infant food, etc.), meat products, fish products, tofu, jelly, noodles (eg, (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, natural flavors (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages and other health supplement foods. The functional food, beverage, food additive or beverage additive may be produced by a conventional production method.

The term "functional food" as used herein refers to a food group that is imparted with added value to function or express the function of the food by physical, biochemical, biotechnological techniques, etc., or to control the biological defense rhythm of the food composition, Means a food which has been designed and processed so as to sufficiently express the body's control function with respect to the living body. Specifically, it may be a health functional food. In particular, the composition of the present invention is suitable for application as an antioxidant-functional food composition. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

In the food composition, the amount of the extract may be 0.00001 wt% or more, specifically 0.1 wt% or more, 800 wt% or less, specifically 50 wt% or less, more specifically 40 wt% or less , And when the food is a beverage, it is contained in a proportion of not less than 0.001 g, specifically not less than 0.01 g, not more than 50 g, specifically not more than 10 g, more specifically not more than 2 g based on 100 ml of the total volume of the food But is not limited thereto.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof. Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Specifically, natural sweeteners are used. Examples of the natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose. Flavors may be used to enhance taste or flavor, both natural and synthetic. Specifically, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used. Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin. As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition. Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid). Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like. Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste. Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

The composition of the present invention is a complex plant extract, and has excellent antioxidative and whitening activities not obtained in each single plant extract. The composition of the present invention is highly utilized as a food composition or a cosmetic composition. When the composition is applied as a food composition, it is particularly useful as an antioxidant functional food composition. When the composition is applied as a cosmetic composition, it is used as a whitening functional cosmetic composition, a whitening functional and antioxidant (anti-aging) .

Fig. 1 shows the results of the antioxidative effects of the mixed extracts (compound extracts) obtained in Experimental Example 1 of the present invention.
Fig. 2 is a graph showing the antioxidative effect of the extract of Ganoderma lucidum extract in Test Example 1 of the present invention.
Fig. 3 shows the results of testing the antioxidant activity of a single extract of Quercus mongolica in Test Example 1 of the present invention.
Fig. 4 shows the results of testing the antioxidative effect of the extract of the alopecia areata in Test Example 1 of the present invention.
FIG. 5 is a graph showing the tyrosinase inhibitory effect of the mixed extract (compound extract) identified in Experimental Example 2 of the present invention.
Fig. 6 shows the test results of tyrosinase inhibitory effect of mulberry leaf single extract in Test Example 2 of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example One: Heat number Preparation of extracts.

The completely dried, mulberry leaf, tree, mongolian and alfalfa were mixed at a weight ratio of 2: 2: 1: 1 1 kg (333 g, 333 g, 167 g and 167 g, respectively) of the mixed sample was put into 10 L of water, and extracted with hot water twice at 85 ° C for 4 hours in an extractor equipped with a condenser. The extract was filtered with 400 mesh filter cloth and concentrated to obtain a hot water extract of about 39 g (dry weight).

At this time, the mulberry leaf, tree, and mongolian tree were extracted using leaves and stems, and shoots were used. The same was applied to Examples 2 and 3 below.

Example 2: Preparation of ethanol extract.

1 kg of a mixed sample of 2: 2: 1: 1 mixture of completely dried, mulberry leaf, alfalfa, Quercus mongolica, and alfalfa was added to 10 L of ethanol and extracted twice at 85 ° C. for 4 hours in an extractor equipped with a cooling condenser . The extract was filtered with 400 mesh filter cloth and concentrated under reduced pressure to obtain an ethanol extract of about 33 g (dry weight).

Example 3: Preparation of methanol extract.

1 kg of a mixed sample prepared by mixing completely dried, mulberry leaf, alfalfa, Quercus mongolica, and alfalfa at a weight ratio of 2: 2: 1: 1 was added to 10 liters of methanol and the mixture was extracted twice with a condenser at 85 ° C for 4 hours Respectively. The extract was filtered with 400 mesh filter cloth and concentrated under reduced pressure to obtain a methanol extract of about 32 g (dry weight).

Experimental Example 1: of the composition containing the extract DPPH Inhibition of free radical scavenging activity.

The ethanol extract prepared in Example 2 was diluted with DMSO to prepare samples at concentrations of 100, 20, 4, 0.8, and 0.16 μg / ml, respectively, and the DPPH free radical scavenging activity was measured according to the concentration.

4 mg of DPPH (1-1-diphenyl-2-picryl-hydrazyl) reagent was mixed with 50 ml of distilled water and stirred for 1 minute to prepare a 0.2 mM DPPH solution. Blank (2 wells) was treated with 100 μl of methanol in a 96-well plate, and 100 μl of methanol and 100 μl of 0.2 mM DPPH solution were added to Control (2 wells).

In the experimental group, 2 μl of the sample prepared above, 100 μl of methanol and 100 μl of 0.2 mM DPPH solution were treated. plate was incubated in an incubator at 25 ° C for 30 minutes and then the absorbance was measured at 517 nm using a microplate counter.

Vitamin C was dissolved in DMSO at a concentration of 10 mg / ml and diluted to 100, 20, 4, 0.8 and 0.16 ㎍ / ㎖, respectively.

- Blank (2 wells): 100 [mu] l 100% MeOH

Control (2 wells): 100 [mu] l 100% MeOH, 100 [mu] l DPPH

- Plant Extract Sample: Sample 2 μl, 100 μl of 100% MeOH, 100 μl of DPPH

And the absorbance reduction rate of the experimental group and the control group of the sample solution. The activity inhibition rate was calculated using the following equation (1). The experimental results are shown in Table 1 and also in the graph of Fig.

[Equation 1]

Inhibition (%)

= 100 - [{OD ₅₁₇ (Sample) -OD ₅₁₇ (Blank)} / {OD ₅₁₇ (Control) -OD (Blank)} x 100]

Water extract sample Sample concentration that eliminates 50% free radicals
(IC ₅₀ , [mu] g / ml) Mulberry leaf, tree, mongolian, and gnat
Mixed extract of 12.1 Vitamin C 2.2

As shown in Table 1 and Fig. 1, the mixed extract of mulberry leaf, alfalfa, Quercus mongolica, and Quercus mongolica had a lower ability to cleave DPPH free radicals than Vitamin C. However, since the antioxidant effect of Vitamin C is well known, it is lower than Vitamin C, but it can be said that it is sufficient to utilize. However, the DPPH free radical scavenging activity (%) of the sample solution dissolved at a concentration of 100 and 20 μg / ml was higher than that of the other diluted sample solutions, and in particular, 100 μg / Concentration DPPH free radical scavenging ability (%) was higher in the concentration of sample solution than other concentrations.

On the other hand, the same antioxidative effect was tested by using the extracts of each of the extracts of Sophora japonica, Quercus mongolica, and Cryptomeria japonica. However, these antioxidative effects were excellent (see FIGS. 2 to 4) (IC ₅₀ ) concentration of 50 占 퐂 / ml or more, indicating that the antioxidant effect is low (not shown).

Therefore, it is estimated that the extract of Mulberry Leaf Pinus densiflora, Pinus densiflora, Quercus mongolica and Mulberry leaf extract according to the present invention inhibits free radicals promoting the skin aging process and can lower the aging process and skin damage.

Experimental Example 2: Tyrosinase inhibitory effect of a composition containing an extract

The tyrosinase inhibitory effect of the combined extracts (compound extract) of the mulberry leaf pitcher, alfalfa leafwood, Quercus mongolica and alfalfa sp. Obtained in Example 2 was measured.

Tyrosinase is an enzyme purified from potato or mushroom. In this experiment, Sigma (Sigma) extracted from mushrooms was used. First, tyrosine, a substrate, was dissolved in a sodium phosphate buffer solution (0.1 M, pH 6.8) to prepare a solution having a concentration of 0.3 mg / ml. 334 占 퐇 of the solution was put into a test tube, Solution (0.1 M, pH 6.8). The combined dry plant extracts of Example 2 were dissolved in methanol to a concentration of 10 mg / ml and diluted to 100, 20, 4, 0.8, and 0.16 μg / ml using DMSO, respectively. Each of the diluted extract samples was taken in an amount of 70 μl each, added to the solution in the test tube, and reacted in a 37 ° C thermostat for 10 minutes.

Specifically, 1M PB (Phosphate Buffer, pH 6.8) was sterilized at 121 ° C for 20 minutes to cool (room temperature: 25 ° C). 1M PB was made to 1/15 M (final concentration = 66.6 mM), and L-DOPA was dissolved in phosphate buffer (pH 6.8) at a concentration of 2.0 mM. Tyrosinase (46 units / ml) was dissolved in phosphate buffer (pH 6.8), and the cells were dispensed into 1.5 ml tubes. Blank (2 wells) was treated with 2ul DMSO and 120ul PB in a 96 well plate. Control (2wells) was treated with 2 μl of DMSO, 120 μl of PB and 40 μl of Tyrosinase. The experimental group was treated with 2 μl of sample, 120 μl of PB and 40 μl of tyrosinase. 40 μl of L-DOPA solution (2 mM) was dispensed into each well, reacted for 10 minutes in a 37 ° C thermostatic chamber, and then the absorbance at 475 nm was measured by an analytical system.

When measuring the inhibitory effect of tyrosinase on arbutin, arbutin was dissolved in DMSO to a concentration of 10 mg / ml, and further diluted with DMSO to give 100, 20, 4, 0.8 and 0.16 占 퐂 / ml Diluted to the experimental group.

- Blank (2 wells): 100 μl DMSO 2 μl, PB 160 μl, not treated

- Control (2 wells): 2 [mu] l of 100% DMSO, 120 [mu] l of PB, 40 [

- Extract sample: 2 100 of 100% DMSO, 120 ㎕ of PB, 40 Ty of tyrosinase

And the absorbance reduction rate of the sample solution and the control. The active inhibition rate was calculated using the following equation (2). The experimental results are shown in Table 2 and also shown in the graph of FIG.

&Quot; (2) "

Inhibition activity (%)

= {1- (Control O.D - Sample O.D) / (Control O.D - Blank O.D)} x100

Plant extract sample A concentration that inhibits tyrosinase activity by 50%
(IC ₅₀ : [mu] g / ml) Mulberry leaf, tree, mongolian, and gnat
Mixed extract of 2.74 Arbutin 87.0

As shown in Table 2, it was evaluated that the extract of Mulberry Leaf Pinus densiflora, Quercus mongolica, Quercus mongolica and Mulberry jelly extract significantly inhibited the activity of tyrosinase by 50% compared to arbutin and showed excellent whitening effect. Further, as shown in Fig. 5, the mixed extracts (compound extracts) of mulberry leafwood, alfalfa, Quercus mongolica and alfalfa were respectively dissolved in a concentration of 20 μg / ml and a concentration of 4 μg / The inhibition rate of the enzyme activity was high. Especially, the inhibitory activity of tyrosinase activity was remarkably higher than that of other concentrations in the sample solution of 20 μg / ml. Furthermore. As a result of confirming the degree of inhibition of tyrosinase activity by extracts obtained by applying each of these plants alone, the activity of mulberry leaf trees showing the most excellent effect was insignificant with an IC 50 value of 50 / / ml or more (see FIG. 6 , Other results not shown).

Therefore, it was confirmed that the extracts of Mulberry Leaf Pinus densiflora, Pinus densiflora, Quercus mongolica, and Quercus mongolica extract according to the present invention effectively inhibited the production of melanin pigment according to the activity of tyrosinase, which plays a key role in the melanin biosynthesis process. In addition, it showed higher whitening effect at a concentration of 20 ㎍ / ㎖ than other mixed extracts, and it was confirmed that the whitening activity was superior to the positive control group, arbutin.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims

Wherein the cosmetic composition contains an extract of a mixture comprising mulberry leafwood, alfalfa, Quercus mongolica, and alfalfa.

The method according to claim 1,
Wherein the extract is contained in an amount of 0.0001 to 30% by weight based on the total weight of the cosmetic composition, and has a combined functionality of whitening activity and antioxidative activity.

Wherein the composition comprises an extract of a mixture comprising mulberry leaf, tree, mung bean tree, and algae.