KR20160144428A - 생물학적 물질 분리를 위한 개선된 장치 - Google Patents
생물학적 물질 분리를 위한 개선된 장치 Download PDFInfo
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
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| EP3129481A4 (en) | 2014-04-08 | 2017-10-25 | Biological Dynamics, Inc. | Improved devices for separation of biological materials |
| EP3158088A4 (en) * | 2014-06-20 | 2017-12-27 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
| KR101701618B1 (ko) * | 2015-06-23 | 2017-02-13 | 국립암센터 | 전도성 고분자를 이용한 세포 유리 dna 검출용 구조체 및 이의 용도 |
| WO2017165852A1 (en) | 2016-03-24 | 2017-09-28 | Biological Dynamics, Inc. | Disposable fluidic cartridge and components |
| PL3442711T3 (pl) * | 2016-04-15 | 2021-11-15 | Fluid-Screen, Inc. | Sposoby wykrywania analitu oraz urządzenie wykorzystujące dielektroforezę i elektroosmozę |
| US9873129B1 (en) * | 2016-12-19 | 2018-01-23 | Charlot Biosciences, Inc. | Multi-planar microelectrode array device and methods of making and using same |
| WO2018208820A1 (en) | 2017-05-08 | 2018-11-15 | Biological Dynamics, Inc. | Methods and systems for analyte information processing |
| WO2019126388A1 (en) | 2017-12-19 | 2019-06-27 | Biological Dynamics, Inc. | Methods and devices for detection of multiple analytes from a biological sample |
| WO2019195196A1 (en) * | 2018-04-02 | 2019-10-10 | Biological Dynamics, Inc. | Dielectric materials |
| US12390810B2 (en) | 2019-08-23 | 2025-08-19 | The Regents Of The University Of California | Electrokinetic microelectrode devices and methods for biomarker analysis |
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| EP4058055A4 (en) | 2019-11-13 | 2023-12-13 | Fluid-Screen, Inc. | APPARATUS AND METHODS FOR RAPIDLY DETECTING, SEPARATING, PURIFYING AND QUANTIFYING VARIOUS VIRUSES CONTAINED IN CELLS, CULTURE MEDIUM AND OTHER LIQUIDS |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6149789A (en) | 1990-10-31 | 2000-11-21 | Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Process for manipulating microscopic, dielectric particles and a device therefor |
| US5632957A (en) | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
| US6403367B1 (en) | 1994-07-07 | 2002-06-11 | Nanogen, Inc. | Integrated portable biological detection system |
| US6071394A (en) | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
| US6641708B1 (en) | 1996-01-31 | 2003-11-04 | Board Of Regents, The University Of Texas System | Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation |
| GB9615775D0 (en) | 1996-07-26 | 1996-09-04 | British Tech Group | Apparatus and method for characterising particles using dielectrophoresis |
| US6749736B1 (en) | 1998-06-26 | 2004-06-15 | Evotec Technologies Gmbh | Electrode arrangement for the dielectrophoretic diversion of particles |
| US6203683B1 (en) | 1998-11-09 | 2001-03-20 | Princeton University | Electrodynamically focused thermal cycling device |
| US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
| CN1181337C (zh) | 2000-08-08 | 2004-12-22 | 清华大学 | 微流体系统中实体分子的操纵方法及相关试剂盒 |
| US6824664B1 (en) | 1999-11-04 | 2004-11-30 | Princeton University | Electrode-less dielectrophorises for polarizable particles |
| US6790330B2 (en) | 2000-06-14 | 2004-09-14 | Board Of Regents, The University Of Texas System | Systems and methods for cell subpopulation analysis |
| CN100392384C (zh) | 2000-10-09 | 2008-06-04 | 清华大学 | 芯片上分离实体分子的方法和样品溶液 |
| US7014744B2 (en) * | 2001-08-24 | 2006-03-21 | Applera Corporation | Method of purification and concentration using AC fields with a transfer tip |
| WO2003052269A1 (en) * | 2001-12-18 | 2003-06-26 | Massachusetts Institute Of Technology | Microfluidic pumps and mixers driven by induced-charge electro-osmosis |
| US6887362B2 (en) | 2002-02-06 | 2005-05-03 | Nanogen, Inc. | Dielectrophoretic separation and immunoassay methods on active electronic matrix devices |
| GB2392977A (en) | 2002-09-13 | 2004-03-17 | Suisse Electronique Microtech | A fluidic dielectrophoretic system and method for analysing biomolecules |
| US7105081B2 (en) | 2002-12-20 | 2006-09-12 | Board Of Regents, The University Of Texas System | Methods and apparatus for electrosmear analysis |
| DE10311716A1 (de) | 2003-03-17 | 2004-10-14 | Evotec Oai Ag | Verfahren und Vorrichtung zur Trennung von Partikeln in einer Flüssigkeitsströmung |
| AU2004262301A1 (en) | 2003-07-25 | 2005-02-10 | Platypus Technologies, Llc | Liquid crystal based analyte detection |
| US7709262B2 (en) | 2004-02-18 | 2010-05-04 | Trustees Of Boston University | Method for detecting and quantifying rare mutations/polymorphisms |
| US20070240495A1 (en) | 2004-05-25 | 2007-10-18 | Shuzo Hirahara | Microfluidic Device and Analyzing/Sorting Apparatus Using The Same |
| ITBO20040420A1 (it) * | 2004-07-07 | 2004-10-07 | Type S R L | Macchina per taglio e formatura di piattine metalliche |
| JP2006047153A (ja) | 2004-08-05 | 2006-02-16 | Sony Corp | Dnaチップの製造方法と製造システム、ハイブリダイゼーション検出方法と検出システム、並びに基板処理装置と基板処理方法 |
| JP4645110B2 (ja) | 2004-09-15 | 2011-03-09 | ソニー株式会社 | 誘電泳動を利用するハイブリダイゼーション検出部と該検出部を備えるセンサーチップ、並びにハイブリダイゼーション検出方法 |
| EP1764418B1 (en) | 2005-09-14 | 2012-08-22 | STMicroelectronics Srl | Method and device for the treatment of biological samples using dielectrophoresis |
| US20070080062A1 (en) * | 2005-10-03 | 2007-04-12 | Harnett Cindy K | Coated metal structures and methods of making and using thereof |
| WO2007102839A2 (en) | 2005-10-27 | 2007-09-13 | Applera Corporation | Optoelectronic separation of biomolecules |
| TWI304752B (en) | 2005-12-09 | 2009-01-01 | Ind Tech Res Inst | Multi-sample microfluidic dielectrophoresis separator |
| KR100745754B1 (ko) * | 2005-12-29 | 2007-08-02 | 삼성전자주식회사 | 금속 기둥 전극 구조를 포함하는 유전 영동을 이용하여입자를 조작하기 위한 장치 및 그를 이용하여 빠른유속으로 유전 영동에 의하여 입자를 조작할 수 있는 방법 |
| DE102006002462A1 (de) * | 2006-01-18 | 2007-07-19 | Evotec Technologies Gmbh | Elektrischer Feldkäfig und zugehöriges Betriebsverfahren |
| JP2009530634A (ja) | 2006-03-21 | 2009-08-27 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | フィールド電極群を備えたマイクロエレクトロニクスデバイス |
| EP2010323B1 (en) * | 2006-04-10 | 2012-11-14 | Technion Research and Development of Foundation, Ltd. | Method and device for electrokinetic manipulation |
| ITTO20060273A1 (it) * | 2006-04-12 | 2007-10-13 | Silicon Biosystem S P A | Metodi ed apparati per la selezione e/o il processamento di particellle, in particolare per la lisi selettiva e/o ottimizzata di cellule |
| KR100813254B1 (ko) | 2006-05-29 | 2008-03-13 | 삼성전자주식회사 | 유전 영동을 통하여 분극성 분석물을 분리하기 위한 장치및 그를 이용하여 시료 중의 분극성 물질을 분리하는 방법 |
| JP2008298575A (ja) | 2007-05-31 | 2008-12-11 | Panasonic Corp | 電極および製造方法とそれを用いた検出装置と検出方法 |
| JP2011516867A (ja) * | 2008-04-03 | 2011-05-26 | ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア | 細胞、小胞、ナノ粒子およびバイオマーカーを分離および単離するためのエキソビボの多次元システム |
| JP5306092B2 (ja) * | 2009-07-17 | 2013-10-02 | キヤノン株式会社 | 流体制御装置 |
| DE102009028493B4 (de) * | 2009-08-13 | 2023-08-24 | Robert Bosch Gmbh | Mikrofluidische Zelle |
| WO2011057347A1 (en) | 2009-11-12 | 2011-05-19 | Tgr Biosciences Pty Ltd | Analyte detection |
| GB201102385D0 (en) | 2011-02-10 | 2011-03-30 | Biocule Scotland Ltd | Two-dimensional gel electrophoresis apparatus and method |
| CN102320559B (zh) * | 2011-09-14 | 2014-06-18 | 上海交通大学 | 一种中空结构的微阵列电极的制备方法 |
| US8932815B2 (en) | 2012-04-16 | 2015-01-13 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
| JP2015519886A (ja) | 2012-04-16 | 2015-07-16 | バイオロジカル ダイナミクス,インク. | 核酸サンプル調製 |
| US20150219618A1 (en) | 2012-07-18 | 2015-08-06 | Biological Dynamics, Inc. | Manipulation of microparticles in low field dielectrophoretic regions |
| WO2014028222A1 (en) | 2012-07-31 | 2014-02-20 | Crown Bioscience, Inc. | Biomarkers for identifying esophageal cancer patients for treatment with an anti-egfr drug |
| US20140066317A1 (en) | 2012-09-04 | 2014-03-06 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| US9551665B2 (en) | 2012-10-01 | 2017-01-24 | National Cheng Kung University | Method for detecting mitochondria gene alterations |
| WO2015038797A1 (en) | 2013-09-13 | 2015-03-19 | The Board Of Trustees Of The Leland Stanford Junior University | Plasmonic beads for multiplexed analysis by flow detection systems |
| EP3129481A4 (en) * | 2014-04-08 | 2017-10-25 | Biological Dynamics, Inc. | Improved devices for separation of biological materials |
| EP3158088A4 (en) | 2014-06-20 | 2017-12-27 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
| US20160327549A1 (en) | 2015-05-04 | 2016-11-10 | Biological Dynamics, Inc. | Particle based immunoassay with alternating current electrokinetics |
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- 2015-04-07 CA CA2945146A patent/CA2945146A1/en not_active Abandoned
- 2015-04-07 CN CN201580030621.7A patent/CN106459966A/zh active Pending
- 2015-04-07 JP JP2016561352A patent/JP2017512483A/ja active Pending
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Also Published As
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|---|---|
| EP3129481A4 (en) | 2017-10-25 |
| JP2017512483A (ja) | 2017-05-25 |
| US9682385B2 (en) | 2017-06-20 |
| CA2945146A1 (en) | 2015-10-15 |
| US20150283553A1 (en) | 2015-10-08 |
| US9387489B2 (en) | 2016-07-12 |
| MX2016013216A (es) | 2017-05-01 |
| EP3129481A1 (en) | 2017-02-15 |
| US20160271622A1 (en) | 2016-09-22 |
| IL248200A0 (en) | 2016-11-30 |
| WO2015157217A1 (en) | 2015-10-15 |
| CN106459966A (zh) | 2017-02-22 |
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| PG1501 | Laying open of application | ||
| PC1203 | Withdrawal of no request for examination |