KR20160075378A - 신규한 돼지 유행성설사 바이러스 및 그의 이용 - Google Patents

신규한 돼지 유행성설사 바이러스 및 그의 이용 Download PDF

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KR20160075378A
KR20160075378A KR1020150183150A KR20150183150A KR20160075378A KR 20160075378 A KR20160075378 A KR 20160075378A KR 1020150183150 A KR1020150183150 A KR 1020150183150A KR 20150183150 A KR20150183150 A KR 20150183150A KR 20160075378 A KR20160075378 A KR 20160075378A
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Abstract

본 발명은 신규한 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주에 관한 것이다. 본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 백신 조성물에 관한 것이다. 또한, 본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물에 관한 것이다. 또한, 본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원, 또는 이를 검출할 수 있는 제제를 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물에 관한 것이다. 또한, 본 발명은 상기 바이러스 생장 억제제를 포함하는, 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물에 관한 것이다. 본 발명에서는 돼지유행성 설사 양성 분변 가검물에서 국내 최초로 현재 유행하고 있는 PED 바이러스 야외주를 분리하였으며, 야외주는 전형적 PEDV의 병원성을 나타내는 바, 이를 바탕으로, 백신 조성물, 치료제, 진단제 등 다양하게 적용될 수 있을 것으로 기대된다.

Description

신규한 돼지 유행성설사 바이러스 및 그의 이용 {Korean porcine epidemic diarrhea virus isolate and use thereof}
본 발명은 신규한 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주에 관한 것이다.
본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 백신 조성물에 관한 것이다.
또한, 본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물에 관한 것이다.
또한, 본 발명은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원, 또는 이를 검출할 수 있는 제제를 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물에 관한 것이다.
또한, 본 발명은 상기 바이러스 생장 억제제를 포함하는, 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물에 관한 것이다.
돼지유행성 설사 바이러스(porcine epidemic diarrhea virus; PED virus)는 자돈에서 급성 장염과 수양성 설사를 유발하며 특히 어린 자돈에 있어서는 폐사율이 100%에 달하는 돼지 바이러스성 원인체이다.
이 질환은 1971년 영국에서 최초로 보고되었으며, 유럽의 양돈국에 빠르게 퍼져나갔다. 1990년대, PED는 유럽에서는 희귀해졌으며, 성체 돼지에서 이유후 설사에 관련이 높아졌다. PED는 1982에 아시아세서 보곡되었으며, 그 후 아시아 양돈산업에 큰 경제적 손해를 야기하였다 (Chen et al., 2008; Kweon et al., 1993; Li et al., 2012; Puranaveja et al., 2009; Takahashi et al., 1983).
2013년 5월, PED가 미국에 창궐하였으며, 캐나다 및 맥시코로 전세계적으로 퍼졌으며, 이에 의하여 신생돈의 대량 사멸 및 거대한 경제적 우려를 야기하였다 (Mole, 2013; Stevenson et al., 2013 Vlasova et al., 2014).
PED의 병원성 유발물인 PED 바이러스 (PEDV)는 1978년도에 코로나 바이러스로 확인되었으며, Nidovirales 목, Coronaviridae 과, Alphacoronavirus 속에 해당한다 (Lai et al., 2007; Pensaert and de Bouck, 1978; Saif et al., 2012).
PEDV는 5' 캡과 3' 폴리 A tail을 가지는 약 28 kb의 단일-가닥 포지티브 센스 RNA 게놈을 포함하고 있는 외막 (enveloped) 바이러스이다 (Pensaert and de Bouck, 1978; Saif et al., 2012). 스파이크 (S, spike) 단백질은 비리온 (virion)의 주요 외피 당단백질로, 바이러스의 진입 및 자연숙주에서의 항체의 중화를 매개하기 위하여, 세포 수용체와 상호작용하는 역할을 수행한다 (Jackwood et al., 2001; Lai et al., 2007; Lee et al., 2010). 이에 따라, PEDV S 당단백질은 PEDV의 분리주의 유전적 관련성을 결정하고나, 진단 어세이 개발 및 백신의 개발에 적합한 바이러스 유전자이다 (Chen et al., 2014; Gerber et al., 2014; Lee et al., 2010; Lee and Lee, 2014; Oh et al., 2014).
한국에서 PED의 유행성을 확인한 것은 1992년이다 (Kweon et al., 1993).
그러나, 후향성 연구결과, PEDV는 이미 1987년 초반에 존재하였다는 것을 알수 있었다 (Park and Lee, 1997).
PED는 매해 발생하였으며, 2010년 초까지 한국 양돈산업에 막대한 경제적 손실을 야기하였다. 2010 내지 2011 기간 동안, FMD (foot-and-mouth disease)가 수회 발생한 후, 한국에서의 PEDV의 감염은 산발적이었다.
그러나, 2013년 11월을 시작으로, PED의 유행이 한국에서 다시 나타났으며, 40%이상의 양돈 농가를 휩쓸었다 (Lee and Lee, 2014; Lee et al., 2014a,b). PED에 대한 생균 또는 사균 바이러스가 한국에 시판되었으나, 지속적인 PED의 유행은 이러한 백신의 낮은 효율을 나타낸다. 이러한 결과는 백신과 야외주간의 S 단백질의 유전적 및 항원적 차이점에서 발생한다 (Lee et al., 2010; Oh et al., 2014; Lee and Lee, 2014). 이에 따라서, 효과적인 백신의 부재는 PED를 조절할 수 있는 다음 세대의 백신의 개발을 필요로 한다.
세포 배양물에서의 PEDV의 동정은 PED 예방을 위한 백신 또는 PEDV의 연구에 매우 필수적이다. 그러나, PEDV의 동정은 매우 어려우며, 동정된 바이러스여도 수회의 세포 계대배양에서 그 감염성을 유지하는 것은 불가능하였다 (Chen et al., 2014). 현재까지, 한국형 PEDV가 20계대까지 배양하였다는 것은 2차례 보고뿐이었으며, 이는 야외 PEDV와 유전적으로 상이한 것이었다 (Kweon et al., 1999; Song et al., 2003).
따라서 현재 유행하고 있는 야외주를 이용한 차세대 백신 개발이 시급한 상황이다. 하지만 실험실적으로 야외주 PED 바이러스 분리 및 배양이 어려워 야외주를 이용한 백신 개발에 난항을 겪고 있다.
이에 본 발명의 발명자는 돼지유행성 설사 양성 분변 가검물에서 국내 최초로 현재 유행하고 있는 PED 바이러스 야외주를 분리하였고 이 야외주에 대한 배양 방법을 포함한 in vitro 특성, 바이러스 유전자 분석 및 in vivo 병원성 특성을 확인함으로써, 본 발명을 완성하게 되었다.
이에 따라, 본 발명의 일 양상은 신규한 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주를 제공하는 것이다.
또한, 본 발명의 일 양상은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 백신 조성물을 제공하는 것이다.
또한, 본 발명의 일 양상은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물을 제공하는 것이다.
또한, 본 발명의 다른 양상은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물을 제공하는 것이다.
또한, 본 발명의 다른 양상은 상기 바이러스 생장 억제제를 포함하는, 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물을 제공하는 것이다.
본 발명의 일 양상은 신규한 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주를 제공한다.
이하 본 발명을 상세히 설명한다.
본 발명자는 돼지유행성 설사병 양성 분변으로부터 국내 최초로 2014년 유행하는 PED 바이러스를 분류하고, 이러한 PEDV 및 계대배양으로 유도된 변이된 바이러스를 수득하였으며, 이에 대한 in vitro 특성 및 in vivo 병원성 특성 및 서열을 특정하였다.
이에 따라 본 발명은 서열번호 1의 아미노산 서열 또는 이와 98% 이상의 상동성을 가지는 아미노산 서열로 이루어지며 동등한 면역원성을 가지는 스파이크 단백질을 포함하는 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주를 제공한다.
본 발명에서 용어, "PEDV(porcine epidemic diarrhea virus)"는 코로나바이러스과(coronaviridae)에 속하며 단일 가닥 RNA를 게놈(genome)으로 가지며 길이는 약 28Kb이고, 3개의 주요 구성 단백질인 spike 단백질(180-220KDa)과 막단백질 또는 외피 단백질 (27-32KDa) 그리고 뉴클레오캡시드 단백질(55-58KDa)을 암호화 하고 있다(Shenyang 2007). 이는 같은 종인 SARS 코로나바이러스와 유사한 구조를 가지며 트립신이 첨가된 아프리카 녹색원숭이 신장세포(Vero cell)에서 배양된다(Kim 2003; Stadler 2003; 권 2009).
본 발명에서 용어, "Spike 단백질"은 PEDV의 주요 구성 단백질로서, 목표 세포를 인식하고 바이러스와 세포막 (cellular membrane)를 융합시키는 생물학적으로 중요한 기능을 가지고 있다(Chang 2002).
본 발명의 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주는 서열번호 1의 아미노산 서열을 포함하거나, 상기 아미노산 서열과 98% 이상의 상동성을 나타내는 아미노산 서열을 포함할 수 있다. 상기 분리주는 서열번호 1의 아미노산 서열과 바람직하게는 98%, 98,5%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8% 또는 99.9%의 상동성을 나타내는 아미노산을 포함할 수 있다. 또한, 서열번호 1의 아미노산 서열과 98% 이상의 상동성을 나타내는 아미노산 서열은 서열번호 1의 아미노산 서열과 동등 또는 상응하는 면역원성을 나타내는 것이 바람직하다.
상기 98% 이상의 상동성을 가지는 아미노산 서열은 서열번호 8, 15, 22, 28, 34, 및 40으로 이루어진 군으로부터 선택된 아미노산 서열일 수 있다.
또한, 상기 분리주는 바람직하게는 서열번호 2의 ORF (open reading frame) 1a/1b, 서열번호 3의 ORF 3, 서열번호 4의 외피 단백질 (envelope protein), 서열번호 5의 막단백질 (membrane protein), 및 서열번호 6의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열 또는 이와 98% 이상의 상동성을 가지는 아미노산 서열을 더 포함하는 것 일 수 있다. 더욱 바람직하게 상기 분리주는 서열번호 7의 아미노산 서열로 이루어진 것일 수 있다. 가장 바람직하게, 상기 분리주는 수탁번호 KCTC12736BP로 수탁된 것일 수 있다. 본 발명자는 신규한 돼지 유행성설사 바이러스 국내 분리주인 KNU-141112 바이러스를 2014년 12월 18일자로 대한민국 대전시 유성구 어은동에 위치한 한국생명공학연구원 미생물자원센터에 기탁하여, 2014년 12월 18일자로 기탁번호 KCTC12736BP를 부여받았다.
또한, 상기 분리주는 서열번호 8의 스파이크 단백질, 서열번호 9의 ORF (open reading frame) 1a/1b, 서열번호 10의 ORF 3, 서열번호 11의 외피 단백질 (envelope protein), 서열번호 12의 막단백질 (membrane protein), 및 서열번호 13의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게는 상기 분리주는 서열번호 14의 아미노산 서열로 이루어진 것일 수 있다.
또한, 상기 분리주는 서열번호 15의 스파이크 단백질, 서열번호 16의 ORF (open reading frame) 1a/1b, 서열번호 17의 ORF 3, 서열번호 18의 외피 단백질 (envelope protein), 서열번호 19의 막단백질 (membrane protein), 및 서열번호 20의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게는 상기 분리주는 서열번호 21의 아미노산 서열로 이루어진 것일 수 있다.
또한, 상기 분리주는 서열번호 22의 스파이크 단백질, 서열번호 23의 ORF 3, 서열번호 24의 외피 단백질 (envelope protein), 서열번호 25의 막단백질 (membrane protein), 및 서열번호 26의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게 상기 분리주는 서열번호 27의 아미노산 서열을 포함하는 것일 수 있다.
또한, 상기 분리주는 서열번호 28의 스파이크 단백질, 서열번호 29의 ORF 3, 서열번호 30의 외피 단백질 (envelope protein), 서열번호 31의 막단백질 (membrane protein), 및 서열번호 32의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게는 상기 분리주는 상기 분리주는 서열번호 33의 아미노산 서열을 포함하는 것일 수 있다.
또한, 상기 분리주는 서열번호 34의 스파이크 단백질, 서열번호 35의 ORF 3, 서열번호 36의 외피 단백질 (envelope protein), 서열번호 37의 막단백질 (membrane protein), 및 서열번호 38의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게 상기 분리주는 서열번호 39의 아미노산 서열을 포함하는 것일 수 있다.
또한, 상기 분리주는 서열번호 40의 스파이크 단백질, 서열번호 41의 ORF 3, 서열번호 42의 외피 단백질 (envelope protein), 서열번호 43의 막단백질 (membrane protein), 및 서열번호 44의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것일 수 있으며, 바람직하게는 상기 분리주는 서열번호 45의 아미노산을 포함하는 것일 수 있다.
상기 서열번호의 정보는 하기 표1과 같다.
아미노산 명칭 서열번호 아미노산 명칭 서열번호
KNU-141112-feces spike protein 1 KNU-141112-P3
spike protein 		22
KNU-141112-feces ORF1a/1b 2 KNU-141112-P3
ORF3		 23
KNU-141112-feces ORF3 3 KNU-141112-P3
envelope protein		 24
KNU-141112-feces envelope protein 4 KNU-141112-P3
membrane protein		 25
KNU-141112-feces membrane protein 5 KNU-141112-P3
nucleocapsid protein		 26
KNU-141112-feces nucleocapsid protein 6 KNU-141112-P3
complete cds		 27
KNU-141112-feces complete genome 7 KNU-141112-P4 spike protein 28
KNU-141112-p5 spike protein 8 KNU-141112-P4 ORF3 29
KNU-141112- p5 ORF1a/1b 9 KNU-141112-P4 envelope protein 30
KNU-141112- p5 ORF3 10 KNU-141112-P4 membrane protein 31
KNU-141112- p5 envelope protein 11 KNU-141112-P4 nucleocapsid protein 32
KNU-141112- p5 membrane protein 12 KNU-141112-P4 complete cds 33
KNU-141112- p5 nucleocapsid protein 13 KNU-141112-P20 spike protein 34
KNU-141112- p5 complete genome 14 KNU-141112-P20 ORF3 35
KNU-141112-p10 spike protein 15 KNU-141112-P20 envelope protein 36
KNU-141112- p10 ORF1a/1b 16 KNU-141112-P20 membrane protein 37
KNU-141112- p10 ORF3 17 KNU-141112-P20 nucleocapsid protein 38
KNU-141112- p10 envelope protein 18 KNU-141112-P20 complete cds 39
KNU-141112- p10 membrane protein 19 KNU-141112-P30 spike protein 40
KNU-141112- p10 nucleocapsid protein 20 KNU-141112-P30 ORF3 41
KNU-141112- p10 complete genome 21 KNU-141112-P30 envelope protein 42
KNU-141112-P30 membrane protein 43
KNU-141112-P30 nucleocapsid protein 44
KNU-141112-P30 complete cds 45
또한, 본 발명의 일 양상은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 백신 조성물을 제공한다.
본 발명에서 용어, “백신”은 생체에 면역을 주는 항원을 함유한 생물학적인 제제로서, 감염증의 예방을 위하여 사람이나 동물에 주사하거나 경구 투여함으로써 생체에 면역이 생기게 하는 면역원 또는 항원성 물질을 말한다. 생체 내 면역은 병원균의 감염 후에 생체 내 면역력이 자동으로 얻어지는 자동면역과 외부에서 주입한 백신에 의하여 얻어지는 수동 면역으로 크게 나누어진다. 자동면역이 면역에 관계하는 항체의 생성 기간이 길고 지속적인 면역력의 특징이 있는 반면, 백신에 의한 수동 면역은 감염증 치료에 즉시 작용하나 지속력이 떨어지는 단점이 있다.
본 발명에서 용어, “면역원” 또는 “항원성 물질”은 상기 바이러스 유래의 펩티드, 폴리펩티드, 상기 폴리펩티드를 발현하는 유산균, 단백질, 상기 단백질을 발현하는 유산균, 올리고뉴클레오티드, 폴리뉴클레오티드, 및 재조합 바이러스로 구성된 군에서 선택된 어느 하나일 수 있다. 구체적인 예를 들면, 상기 항원 물질은 불활성화된 전체 또는 부분 바이러스 제제 형태, 또는 통상적인 단백질 정제, 유전 공학 기법 또는 화학 합성에 의해 수득되는 항원 분자 형태일 수 있다.
상기 항원의 함량은 백신 조성물 총 중량에 대해서 1 내지 10 중량%일 수 있으며, 바람직하게는 5% 중량이내 일 수 있다.
본 발명에 따른 백신 조성물은 안정제, 유화제, 수산화알루미늄, 인산알루미늄, pH 조정제, 계면활성제, 리포솜, 이스콤(iscom) 보조제, 합성 글리코펩티드, 증량제, 카복시폴리메틸렌, 세균 세포벽, 세균 세포벽의 유도체, 세균백신, 동물 폭스바이러스 단백질, 서브바이랄(subviral) 입자 보조제, 콜레라 독소, N, N-디옥타데실-N',N'-비스(2-하이드록시에틸)-프로판디아민, 모노포스포릴 지질 A, 디메틸디옥타데실-암모늄 브로마이드 및 이의 혼합물로 구성된 군에서 선택된 어느 하나 이상의 제 2 보조제를 추가로 함유하는 것을 특징으로 할 수 있다.
또한, 본 발명의 백신 조성물은 수의학적으로 허용 가능한 담체를 포함할 수 있다. 본 발명에서 용어, "수의학적으로 허용 가능한 담체"란 임의의 및 모든 용매, 분산 매질, 코팅제, 항원보강제, 안정제, 희석제, 보존제, 항균제 및 항진균제, 등장성 작용제, 흡착지연제 등을 포함한다. 백신용 조성물에 포함될 수 있는 담체, 부형제, 희석제로는 락토즈, 덱스트로스, 슈크로스, 솔비톨, 만니톨, 자일리톨, 말티톨, 전분, 글리세린, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘포스페이트, 칼슘실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 들 수 있다.
또한, 본 발명의 백신용 조성물은 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. 제제화할 경우에는 보통 사용되는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제할 수 있다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러 한 고형제제는 상기 레시틴 유사 유화제에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트 (calcium carbonate), 슈크로스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제할 수 있다. 또한 단순한 부형제 이외에 마그네슘 스티레이트 탈크 같은 윤활제들도 사용할 수 있다. 경구투여를 위한 액상제제로는 현탁제, 내용액제, 유제, 시럽제 등을 사용할 수 있으며, 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구투여를 위한 제제에는 멸균된 수용액, 비수용성제, 현탁제, 유제, 동결건조제제가 포함된다. 비수용성제제, 현탁제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다.
상기 상기 백신은 다양한 형태로 개체에 주입될 수 있다. "주입"은 피하주사, 근육내 주사, 피하내 주사, 복막내 주사, 비강투여, 구강투여, 경피투여 및 경구투여로 구성된 군에서 선택된 어느 하나의 방법으로 수행될 수 있다.
또한, 본 발명에서 용어, "주사" 또는 "투여"는 투여대상의 나이, 성별, 체중 등에 따라 투여량이 달라질 수 있고, 투여경로, 질병의 정도, 성별, 체중, 나이 등에 따라서도 백신의 투여량이 본 발명의 백신용 면역증강제는 비경구, 점막(경구 및 비강 등) 및 경피성 경로에 의한 백신 투여시 함께 사용할 수 있다.
본 발명의 백신조성물은 면역반응을 개선 또는 강화시키기 위하여 하나 이상의 보조제 등을 포함할 수 있다. 적절한 보조제에는 펩티드, 알루미늄 하이드록시드, 알루미늄 포스페이트, 알루미늄 옥시드 및 Marcol 52 같은 미네랄 오일 또는 식물성 오일 및 하나 이상의 유화제로 구성된 조성물 또는 리졸세시틴, 다가 양이온, 다가 음이온 같은 표면 활성물질 등이 포함된다.
본 발명의 백신 조성물은 1종 이상의 면역증강제를 추가로 포함할 수 있고, 바람직하게는 콜레라 톡신(CT; cholera toxin), 알루미늄하이드록사이드, 카보폴(carbopol), 광물성 오일 또는 생분해성(Biodegradable) 오일을 포함할 수 있다.
본 발명에서의 용어 "면역증강제"란, 일반적으로 항원에 대한 체액 및/또는 세포 면역 반응을 증가시키는 임의의 물질을 지칭한다. 전통적인 백신들은 죽은 병원성 미생물의 비가공 제제로 구성되어, 병원성 미생물의 배양액과 관련된 불순물들은 면역 반응을 향상시키기 위한 면역증강제로서 작용할 수 있으나, 정제된 단백질 서브유닛의 균질 제제를 백신접종을 위한 항원으로서 사용하는 경우, 상기와 같은 항원에 의해 발동된 면역성은 불충분하여 면역증강제로서 일부 외래물질의 첨가가 필요해 진다. 면역증강제를 사용함으로써 면역 반응을 자극하는 데 보다 적은 용량의 항원이 요구될 수 있으며, 이에 의해 백신 생산 비용이 절감될 수 있다. 이러한 면역증강제는 그 원료(미네랄, 세균, 식물)와 성분(유제현탁액)에 따라 분류된다. 상업적으로 이용되는 면역증강제는 알루미늄하이드록사이드, 카보폴(carbopol), 광물성 오일(미네랄오일) 또는 생분해성(Biodegradable) 오일등이 있다.
또한, 상기 백신은 생균백신 또는 사균백신일 수 있다.
생균백신 또는 생백신은 세균이나 바이러스를 병원성을 약하게 하여 동물에 투여되었을 때 질병의 임상적 증상이 없거나 감소되었음을 나타내는 바이러스를 의미하는 것으로, 본 발명의 약독화주는 당업계에 알려져 있는 방법, 예를 들어, 바이러스의 계대배양을 통해 분리될 수 있다.
사균백신 또는 사독 백신은 세균이나 바이러스를 가열(56~60℃, 30~60분) 또는 포르말린이나 페놀 등의 화학약품에 의해서 면역원성을 잃지 않고 병원성을 불활성으로 한 것이다. 바이러스의 불활화는 당업계에 알려진 방법에 의할 수 있다.
또한 본 발명의 일 양상은, 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물을 제공한다.
본 발명에서 용어, “예방”은 본 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 하는 조성물의 투여로 상기 PEDV 감염을 억제 또는 지연시키는 모든 행위를 말한다.
본 발명에서 용어, “치료”는 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 하는 조성물의 투여로 상기 PEDV의 감염 증상이 호전되거나 이롭게 되는 모든 행위를 말한다.
상기 약학적 조성물은 예를 들면 사료 첨가제로 이용될 수 있다.
본 발명의 사료는 본 발명에 따른 사료 첨가제를 유효성분으로 포함하는 한 당업계에 공지된 다양한 형태의 조성비로 당업자에 의해 적절하게 구성될 수 있으며, 바람직하게는 단백질 20%, 지방, 에테르 추출물 4.5%, 지방, 산 가수분해 5.4%, 조섬유질 4.7%, 회분 6%, 칼슘 0.80% 및 인산염 0.62%를 포함할 수 있다.
또한 본 발명의 일 양상은 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원 또는 이를 검출할 수 있는 제제를 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물을 제공한다.
상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 검출할 수 있는 제제는 바람직하게는 상기 바이러스, 상기 바이러스의 배양물 또는 이의 항원에 특이적인 항체일 수 있다.
본 발명에서 용어, “항체”는 면역계 내에서 항원의 자극에 의하여 만들어지는 물질로서, 면역글로불린이라고도 하며, 특정한 항원과 특이적으로 결합하여 림프와 혈액을 떠돌며 항원항체반응을 일으킨다. 항체가 특이적 항원에 대한 특이성을 나타내는 반면에, 면역글로불린은 항체, 및 항원 특이성이 결여된 항체 유사 물질모두를 포함한다. 후자의 폴리펩티드는 예컨대 림프계에서는 낮은 수준으로 생산되며 골수종에 의해서 증가된 수준으로 생산된다. 본 발명에서는 상기 PEDV 자체, spike 단백질, 또는 이에 포함된 에피토프일 수 있다.
상기 항체는 모노클로날 또는 폴리클로날 항체일 수 있다.
또한, 본 발명의 일 양상은 상기 바이러스 생장 억제제를 포함하는, 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물을 제공한다.
상기 바이러스의 생장 억제제는 바이러스, 상기 바이러스의 배양물 또는 이의 항원에 대한 siRNA(small interference RNA), shRNA(short hairpin RNA), miRNA(microRNA), 리보자임(ribozyme), DNAzyme, PNA(peptide nucleic acids), 안티센스 올리고뉴클레오타이드, 항체, 앱타머, 천연 추출물 및 화학물질 등일 수 있다. 바람직하게는 항체일 수 있다.
본 발명에서는 돼지유행성 설사 양성 분변 가검물에서 국내 최초로 현재 유행하고 있는 PED 바이러스 야외주를 분리하였으며, 야외주는 전형적 PEDV의 병원성을 나타내는 바, 이를 바탕으로, 백신 조성물, 치료제, 진단제 등 다양하게 적용될 수 있을 것으로 기대된다.
도 1. Vero 세포에 감염시킨 PEDV 분리주 KNU-141112의 세포병리학 및 IFA 결과를 나타낸 도이다. Vero 세포는 위-감염되거나, PEDV KNU-141112-P5 및 KNU-141112-P10 분리주로 감염되었다. PEDV-특이적 CPE를 매일 관찰하였으며, 24 hpi시에 200x의 배율에서 도립현미경을 이용하여 촬영하였다 (제1 패널). 면역염색을 위하여, 감염된 세포를 24 hpi시에 고정하고, N 단백질에 대한 Mab와 함께 인큐베이션 하고, Alexa green-접합된 염소 항-마우스 이차 항체로 인큐베이션하였다 (제2 패널). 뒤이어 세포를 DAPI로 대비염색하였으며 (제3 패널), 200x 배율에서 형광변미경으로 확인하였다.
도 2. Vero 세포에서의 KNU-141112의 성장동역학을 나타낸 도이다.
(A) 선택된 패시지에서의 바이러스 역가. Vero 세포를 명시한 패시지 번호에서 회수한 PEDV KNU-141112로 감염시켰다. 24 또는 48 hpi에서, 바이러스 상층액을 수집하고, 바이러스 역가를 결정하였다.
(B) KNU-141112 주의 1단계 증식곡선을 나타낸 도이다. Vero 세포를 PEDV KNU-141112-P10, -P20, 및 -P30로 독립적으로 감염시켰다. 감염 후 명시된 시점에서, 배양 상층액을 회수하고, 바이러스 역가를 결정하였다. TCID50 (50% tissue culture infectious dose)를 계산하였다. 결과를 3개의 웰에서의 평균값으로 나타내었으며, 에러바는 표준편차를 의미한다.
도 3. PEDV KNU-141112 분리주로 접종된 기니피그의 혈청 내의 바이러스중화항체 역가를 나타낸 도이다. 기니피그를 불활화된 KNU-141112-P10의 피하 투여로 2회 면역조치하였다. 혈액 샘플을 면역조치 전 수집하고, 2차 면역조치 2주 후, 동종 (KNU-141112; 원) 및 이종 (SM98-1; 마름모) 바이러스를 이용하여 바이러스 중화 어세이를 수행하였다. 감염된 동물 각각의 중화 항체 역가를 log2값으로 점으로 표시하였다. 값은 2회의 3개의 독립된 실험의 평균으로 나타내며, 에러바는 표준편차를 의미한다.
도 4. 한국 PEDV주 KNU-141112를 접종한 되지의 장의 미시적 및 거시적 관찰결과를 나타낸 도이다. (A) 감염된 돼지의 2 DPI에서의 소장은 얇고, 투명한 장벽을 나타내며, 응유로 가득찬 팽창된 위를 나타낸다.
(B 및 C) KNU-141112-접종된 돼지의 3DPI에서의 헤마톡실린 및 에오신 염색된 공장 (jejunum) (각각 100x 및 200x 배율).
(D) 3 DPI의 돼지로부터 분리된 공장 조직 조작의 IHC 분석 (100x 배율).
(E 및 F) KNU-141112로 접종한 돼지의 3 또는 4 DPI에서의 소장 절편의 면역형광 염색 결과. 절편은 DAPI로 대비염색하였으며, 400x 배율로 형광 현미경을 이용하여 확인하였다.
도 5. PEDV주의 스파이크 유전자 (A) 및 전장 게놈의 뉴클레오티드 서열을 기반으로 한 계통발생 분석결과를 나타낸 도이다. TGEV의 스파이크 단백질의 잠정적 유사 부위 및 완전 게놈 서열을 각 패널 내 outgroup으로 포함시켰다. 다중-시퀀싱 얼라인먼트를 ClustalX 프로그램을 이용하여 수행하였으며, 얼라인된 뉴클레오티드 서열로 인접-결합 방법 (neighbor-joining method)을 이용하여 계통수를 제조하였다. 각 가지의 수는 1000 replicate의 50% 이상의 부트스트랩 (bootstrap)값을 나타낸다. 바이러스주의 명칭, 국가, 및 분리년도, GeneBank accession number, 및 genogroups 및 subgroups를 나타내었다. 본 발명에서 규명한 PEDV 분리주는 원형으로 표시하였다. 스케일바는 각 위치당 뉴클레오티드 치환을 의미한다.
이하 본 발명을 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
실시예 1. 시료 및 방법
1.1. 세포, 임상 샘플, 바이러스 및 항체
Vero 세포 (ATCC CCL-81)를 5% FBS (Invitrogen) 및 antibiotic-antimycotic solutions (100x; Invitrogen) 를 포함하는 α- MEM에서 배양하고, 5% CO2 인큐베이터에서 37 ℃에서 유지시켰다. i-TGE/PED Detection Kit (iNtRON Biotechnology, Seongnam, South Korea)를 이용한 RT-PCR에 의하여 양성으로 확인된 7개의 소장 분쇄물 및 50개의 대변 표본을를 바이러스 분리 실험을 위하여 선별하였다. 소장 분쇄물을 MagNA Lyser (Roche Diagnostics, Mannheim, Germany)를 3회 15초간 7,000 rpm 속도로 실시하여 PBS 내 10% (wt/vol) 현탁액으로 준비하였다. 분변 샘플은 PBS 내에 10% (wt/vol) 현탁액으로 희석하였다. 뒤이어 현탁액을 교반하고, 10분간 4,500xg (Hanil Centrifuge FLETA5, Incheon, South Korea)로 원심분리하였다. 상청액을 0.22-μm 주사기 필터 (Millipore, Billerica, MA)를 통하여 필터링 하고, 바이러스 분리주의 접종원으로서 사용하기 전까지 -80℃에서 보관하였다.
PEDV의 바이러스 분리주를 Vero 세포로 적용하였다. 구체적으로, 6-웰 플레이트에서 생장시킨 컨플루언트 Vero 세포를 PBS로 세척하고, 10 ㎍/ml의 트립신 (USB, Cleveland, OH)을 포함하는 400 ㎕의 샘플로 접종하였다. 1시간동안 37℃에서 인큐베이션 한 후, 2ml의 바이러스 생장 배지 [항생-항진균 용액, 0.3% TPB (Sigma, St. Louis, MO), 0.02% 효모추출물(Difco, Detroit, MI), 10 mM HEPES (Invitrogen), 및 5 μg/ml의 트립신이 보충된 α- MEM]를 첨가하였다. 접종된 세포는 5% CO2 하에서 37 ℃에서 유지시켰으며, CPE (cytopathic effects)를 매일 관찰하였다. 70% CPE가 나타났을 때, 접종된 세포를 3회 동결 융해하였다. 뒤이어 배양 상층액을 수집하고 400xg에서 10분간 원심분리하였다. 정제된 상층액을 부분표본화 (aliquoted)하고, 사용되기 전까지 “P1 (passage 1)”으로서 -80℃에서 보관하였다.
100 밀리미터 직경의 조직 배양 디쉬를 분리의 다수의 패시지에 사용하였다.
7일 간 접종한 세포가 CPE를 보이지 않는 경우, 플레이트를 3회 동결 및 융해하고, 원심분리를 이용하여 상층액을 회수하고, 다음 패시지를 위하여 신선한 Vero 세포에 접종하였다. 6회 블라인드 패시지 후에 CPE 및 RT-PCR의 결과가 음성인 경우, 바이러스 분리주가 음성인 것으로 간주하였다. PEDV N 단백질-특이적 단클론 항체 (MAb)를 ChoogAng Vaccine Laboratory (CAVAC; Daejeon, South Korea)로부터 수득하였다.
1.2 바이러스 역가 측정 (Virus titration)
트립신 존재하에서 상기 기술한 바와 같이 각 패시지의 KNU-141112 바이러스 스톡을 Vero 세포에 감염시켰다. 70% CPE가 일반적으로 발휘되는 때, 24 또는 48시간 후감염 (postinfection (hpi)) 후 배양 상층액을 수집하였다. 성장 동역 실험을 위하여, 상이한 시점 (6, 12, 24, 36, 및 48 hpi)에서 각 선택된 패시지의 바이러스로 감염된 세포로부터 상층액을 수집하고 - 80℃에서 보관하였다.
바이러스 역가는 96-웰 플레이트 상에서 샘플의 10 배 단계 희석물을 이용하여 희석물 당 3차례씩 측정하였으며, 이는 감염된 Vero 세포의 50% CPE를 형성하는데 요구되는 바이러스의 양을 측정하며, Reed-Muench 방법 (Reed and Muench, 1938)를 이용하여 ml당 TCID50 로 계산하였다. 또한 PEDV 역가를 플라크 어세이로, Vero 세포를 이용하여 결정하였으며, ml당 PFU (plaque-forming unit)으로 결정하였다.
1.3 면역형광 어세이 ( IFA , Immunofluorescence assay)
6-웰 조직 배양 플레이스 상에 위치한 현미경 커버슬라이드 상에서 생장한 Vero 세포를 0.1 MOI (multiplicity of infection)에서 PEDV로 위감염 또는 감염시켰다. 뒤이어 바이러스-감염된 세포를 24 hpi까지 번식시키고, 4% 파라포름알데히드로 10분간 실온에서 고정하고, PBS 내 0.2% Triton 1 X-100으로 실온에서 10분간 삼투화 (permeabilized)하였다. 세포를 PBS 중 1% BSA로 30분간 실온에서 블로킹한 뒤, 2시간동안 N-특이적 Mab와 함께 인큐베이션하였다. PBS로 5회 세척후, 세포를 1시간동안 RT에서 Alexa Fluor 488 (Invitrogen)와 접합된 염소 항-마우스 이차 항체와 인큐베이션하고, 뒤이어, DAPI (4?,6-diamidino-2-phenylindole, Sigma)로 대비염색하였다. 커버슬라이드를 마운팅 버퍼 (mounting buffer) 내 현미경 글라스 슬라이드 상으로 마운팅하고, 세포 염색을 형광 Leica DM IL LED 현미경 (Leica, Wetzlar, Germany)을 이용하여 시각화하였다.
1.4. 정량적 실시간 RT- PCR
상기 기술된 바와 같이 준비된 바이러스 분리주 또는 분변 세포로부터 i-TGE/PED Detection Kit를 이용하여 바이러스의 RNA를 추출하였다. 정량적 실시간 RT-PCR을 One Step SYBR PrimeScript RT-PCR Kit (TaKaRa, Otsu, Japan)를 이용하여 실시하였다. 반응을 Thermal Cycler Dice Real Time System (TaKaRa)를 이용하여 수행하였으며, 결과를 Sagong and Lee, 2011에서 기술한 시스템을 이용하여 분석하였다.
1.5 기니피그의 면역조치 (Immunization)
8마리의 기니피그를 접종군 (n=6) 및 대조군 (n=2)으로 임의로 배분하였다. 6마리의 기니피그에 0.5 ml의 BEI (binary ethylenimine)-불활화된 KNU-141112-P10 바이러스를 프로인드 완전 어쥬번트 (Freund? complete adjuvant, Sigma)의 존재하에서 피하로 면역조치하고, 2주 간격으로, 신선하게 제조된 불활화된 바이러스 및 프로인드 완전 어주번트의 에멀전으로 1회 부스팅 (boost)하였다. 2개의 위-접종된 기니피그는 각각의 어주번트 존재 하에서 세포 배양 배지를 투여 및 부스팅하였다. 전면역 (pre-immune) 혈청을 면역조치의 시작 전 수집하고, 항혈청을 최종 부스팅 후 2주경에 수집하였다.
1.6 혈청 중화
모든 군의 기니피그로부터 수집한 혈청 샘플 내 PEDV-특이적 중화 항체의 존재여부를 96-웰 마이크로타이터 (microtiter) 플레이트 내에서 PEDV 분리주 KNU-141112 또는 백신주 SM98-1를 이용한 혈청 중화 실험으로 결정하였다. 구체적으로, Vero 세포를 96-웰 조직 배양 플레이트 내 2x104/웰에서 1일간 생장시켰다. KNU-141112-P10 바이러스 스톡을 무혈청 α-MEM으로 희석시켜 50 ㎕ 부피 내 200 TCID로 만들었다. 뒤이어 96-웰 플레이트에서 개별적 불활화된 혈청의 50 ㎕의 2-fold 단계희석물과 상기희석된 바이러스를 혼합하였다. 혼합물을 Vero 세포 내에 접종하고, 37 ℃에서 1시간동안 인큐베이션 하였다. 혼합물을 제거하고, 세포를 5회 PBS로 세척하고, 2일간 5% CO2 인큐베이터 내에서 37℃에서 바이러스 생장 배지내에 유지시켰다. 백신주를 이용한 혈청 중화 실험을 위하여, 트립신 존재하에서 번식된 PEDV주 SM98-1를 무혈청 α-MEM로 희석하여 50 ㎕ 부피로 200 PFU를 만들었다. 뒤이어 96-웰 플레이트에서 상기 기술된 바와 같이 희석된 바이러스를 각 혈청과 혼합하고, 1시간동안 37℃에서 인큐베이션 하였다. 뒤이어, 5% FBS를 포함하는 100㎕의 α-MEM 중 약 1x104 Vero 세포를 각 웰에 첨가하고 혼합물을 37℃에서 3일간 CO2 인큐베이터 내에서 유지시켰다. 중화 역가를 모든 이중 웰에서 바이러스 특이적 CPE를 억제하는 가장 높은 혈청 희석물의 레시프로컬 (reciprocal)로 계산한다.
1.7 돼지 감염 실험
In vivo 돼지 실험을 동물실험윤리위원회의 가이드라인에 따라 ImproAH Animal Facility에서 실시하였다. PED가 발별하거나 PEDV로 예방접종되지 않은 총 8마리의 7일령 젖먹이 돼지를 구매하였다. 모든 동물이 PEDV 뿐만 아니라, TGEV (transmissible gastroenteritis virus) 및 돼지번식호흡장애 증후군 바이러스 (porcine reproductive and respiratory syndrome virus)에 대한 항체가 없음을 확인하였다. 돼지를 3개의 실험군으로 임의로 분류하고 2개의 분리된 방에서 사육하였다: 1번 방에는 PEDV-접종군 (n = 4) 및 접촉 대조군 (n = 2)을, 2번 방에는 위-접종대조군 (n = 2)을 사육하였다. 하루간의 순응기간 후, PEDV-접종군의 1마리의 돼지에 1 ml 용량의 103 TCID50/ml KNU-141112-P10 바이러스를 경구투여하였다. 2마리의 돼지는 같은 방에서 사육되어 접종된 돼지에 직접 접촉함으로써 바이러스에 노출시켰다. 위-접종 돼지에는 세포 배양 배지를 플라시보로 투여하였다.
설사 및 폐사의 임상 증상을 매일 모니터링하였다. 모든 군의 배변 샘플을 접종 및 매일 16인치의 면봉으로 수집하고, i-TGE/PED Detection Kit를 이용한 RT-PCR 및 실시간 RT-PCR을 수행하였다. PEDV-접종된 돼지를 사후 부검을 위한 공격 (challenge) 후 매일 (1, 2, 3, 및 4일) 부검하였으며, 반면 접촉 및 대조군의 모든 돼지는 사후 부검을 위한 사후-공격 (challenge) 4일경에 안락사시켰다. 각각의 돼지로부터 수집된 내장 조직 표본 (<3mm thick)를 10% 포르말린으로 24시간동안 실온에서 고정하고, 파라핀에 내장화하였다. 포르말린으로 고정되고 파라핀 내에 내장화된 조직을 마이크로톰 (Leica) 상에서 5-8 ㎛ 두께로 자르고, 증류수를 포함하는 40 ℃의 워터베스에서 부유시킨 후, 글라스 슬라이드 위로 이동시켰다. 조직을 자일렌 내에서 5분간 탈파라핀화하고, 각각 3분 100%, 95%, 90%, 80%, 및 70%의 감소하는 농도의 에탄올로 세척하였다. 탈파라핀화된 장 조직 절편을 헤마톡시린 및 에오신 (sigma)로 염색하고, PEDV N-특이적 Mab를 이용하여 면역염색 어세를 실시하였다.
1.8. 면역조직염색 ( Immunohistochemistry )
파라핀내에 내장화된 조직 절편을 탈파라핀화하고, 0.01 M 구연산염 버퍼 (pH 6.0)를 처리하고 5분간 전자레인지에 돌린 후, 실온에서 20분간 식힌 후, DW 중 0.3% 과산화수소와 함께 20분간 인큐베이션하여 내생적 퍼옥시다아제를 블로킹 하였다. PBS로 3회 세척 후, 절편을 정상 말혈청 (VECTASTAIN ABC Kit; Vector Laboratories, Burlingame, CA)으로 블로킹하고, 1시간동안 실온에서 N-특이적 Mab와 함께 인큐베이션하였다. PBS로 세척후, 샘플을 45분간 실온에서 말 항-마우스 이차 항체 (VECTASTAIN ABC Kit)와 반응시키고, 45분간 아비딘-비오틴 페록시다제 복합체 (VECTASTAIN ABC Kit)와 인큐베이션하고, DAB substrate Kit (Vector Laboratories)를 이용하여 발현시켰다. 뒤이어 슬라이드를 헤마톡실린으로 대비염색하고, 건조하고, 자일렌으로 세척후, 마운팅 버퍼 내의 현미경의 글라스 슬라이드 상에 마운팅하고 조직 염색을 현미경으로 시각화 하였다.
1.9. 뉴클레오티드 시퀀싱
원천의 분변 샘플 및 이의 P5 및 P10의 분리주의 완전 게놈 서열을 MGS (next-generation sequencing)기술을 이용하여 결정하였다. 분변 및 P5 및 P10 분리주로부터 총 RNA를 RNeasy Mini Kit (Qiagen, Hilden, Germany)를 이용하여 추출하고, 이를 cDNA 조각을 증폭하기 위한 주형으로 사용하였다.
각 샘플의 전체 게놈을 포함하도록 10개의 오버랩되는 cDNA 조각을 생성하고, 동몰량으로 모은 뒤, Ion Torrent Personal Genome Machine (PGM) Sequencer System (Life Technologies, Carlsbad, CA) 및 316 v2 sequencing chip (Life Technologies)를 이용하여 NGS를 수행하였다. 단일-뉴클레오티드 변이 (single-nucleotide variants, SNVs)를 CLC Genomic Workbench version 7.0 (CLC Bio, Cambridge, MA)을 이용하여 분석하고, 개별적 NGS 판독물을 PEDV 참조주 KOR/KNU-1305/2013 (Genbank accession no. KJ662670)를 이용하여 취합하였다.
분변 및 P5 및 P10의 분리주의 게놈의 5' 말단 및 3' 말단을 RACE (rapid amplification of cDNA end)를 이용하여 결정하였다.
KNU-141112-feces, KNU-141112-P5, and KNU-141112-P10의 전장 게놈 뉴클레오티드 서열을 각각 KR873431, KR873434, 및 KR873435의 accession number로 GenBank에 기탁하였다. 바이러스 분리주의 S 당단백 유전자 서열을 전형적 Sanger 방법으로 결정하였다. 각 분리주의 S 유전자를 포괄하는 2개의 오버랩핑되는 cDNA 조각을 RT-PCR로 증폭하였다. 개별적 cDNA 증폭체 (amplicon)를 겔-정제하고, pGEM-T easy (Promega, Madison, WI)내로 클로닝 하고, 벡터-특이적 T7 및 SP6 프라이머 및 S 유전자-특이적 프라이머를 이용하여 양쪽 방향으로 시퀀싱하였다.
또한, 선택된 패시지의 바이러스 분리주 ((KNU-141112-P3, KNU-141112-P4, KNU-141112-P20, 및 KNU-141112-P30)의 완전 구조 유전자 시퀀스를 Sanger 방식으로 결정하고, 도 5에 나타난 accession number로 GenBank 데이터베이스에 기탁하였다.
1.10 개통발생 분석 (Phylogenetic analysis)
42개의 완전히 시퀀싱된 S 유전자 서열 및 PEDV 분리주의 25개의 완전 게놈에 대하여 독립적으로 시퀀스 얼라이먼트 (sequence alignments) 및 계통발생 분석을 수행하였다. 다중-시퀀스 얼라이먼트를 ClustalX 2.0 program (Thompson et al., 1997)을 이용하여 생성하였으며, 뉴클레오티드 시퀀스 다이버젼스(divergences)의 퍼센트를 동일한 소프트웨어로 측정하였다.
개통수를 인접-결합 방법 (neighbor-joining method)을 이용하여 얼라인된 뉴클레오티드 또는 아미노산 서열로부터 구성하였으며, 뒤이어 복제 (replicate)를 이용한 부트스트랩 (bootstrap) 분석을 수행하여, 계통수의 각 내부 노드 (internal node) 상의 신뢰값 퍼센트를 결정하였다. 모든 계통수 도면은 Mega 4.0 software (Tamura et al., 2007)를 이용하여 생성하였다.
1.11 통계적 분석
모든 통계학적 분석을 위하여 Student? t test를 이용하였으며 0.05 보다 작은 p-value을 유의적인 것으로 간주하였다.
실시예 2. 결과
2.1 바이러스 분리 및 in vitro 특정
본 발명자는 50개의 분변 및 7개의 장 분쇄물을 포함하는, PCR-양성 임상 샘플로부터 Vero 세포 상에 PEDV를 분리고자 하였다. 그중 2014년 9월 29일경 수득한 경상북도에 위치한 농장으로부터 자연적으로 감염된 돼지의 분변으로부터, KNU-141112로 명명된 일 PEDV 분리주를 성공적으로 분리하였다.
또한, 본 발명자는 신규한 돼지 유행성설사 바이러스 국내 분리주인 KNU-141112 바이러스를 2014년 12월 18일자로 대한민국 대전시 유성구 어은동에 위치한 한국생명공학연구원 미생물자원센터에 기탁하여, 2014년 12월 18일자로 기탁번호 KCTC12736BP를 부여받았다.
KNU-141112 바이러스는 감염된 Vero 세포에서 패시지 3 (P3)부터 세포 융합, 합포체 (syncytium) 및 탈리 (detachment) 등 PEDV 감염에서 전형적인 분명한 CPE를 나타내었다.
뒤이어, 이 분리주가 세포 배양에서 효과적으로 배양되며 유지되는지를 확인하였다. 이에 따라, 분리된 PEDV 주 KNU- 141112를 Vero 세포에서 총 30 패시지동안 연속 계대배양하였다 (P1부터 P30). CPE 개시 시점은 24 hpi인바, 이에 따라, 현저한 CPE는 48 hpi 이내에, 첫 2 productive passage (P3 및 P4)에서 관찰되었다. 이후 패시지에서, 가지적인 CPE는 12 hpi에서 나타났으며, 24 hpi에서 뚜렷하였다. 바이러스 증식을 PEDV N 단백질-특이적 Mab를 이용한 IFA에 의하여 PEDV 항원을 검출함으로써 확인하였다. 뚜렷한 염색이 전형적인 합포체 (syncytial) 세포의 세포질에 분포하였다. 대조적으로, 위-접종된 Vero 세포에서는 CPE 및 N-특이적 염색이 나타나지 않았다. 선택된 패시지에서의 CPE의 예시 및 관련된 IFA 이미지를 도 1에 나타내었다.
선택된 패시지에서의 바이러스 게놈의 수준을 실시간 RT-PCR로 측정하고 평균 Ct (cycle threshold) 값을 15.3 (P10) 내지 18.7 (P5) 범위의 16.7로 결정하였다.
분리주의 P5까지의 감염적 역가를 105.1 내지 106.1 TCID50/ml 범위로 정하고, 이후 패시지에서는 약 107 TCID50/ml로 결정하였다. 패시지 10부터 피크 바이러스 역가는 107.8 TCID50/ml (107.5 PFU/ml와 상응)에 이르렀다 (도 2A).
또한, 성장 동역 실험은 KNU-141112가 Vero 세포에서 빠르고 효과적으로 복제되고, 24 dpi에서 최대역가 >107 TCID50/ml에 도달한다는 것을 나타내었다.
2.2. 기니피그에서의 KNU -141112의 항체 반응
면역조치 전 (전면역, pre-immune) 기니피그의 항혈청을 수집하고, 최종 부스트 후 2주 후 최종 부스트 (boost) 및 분리된 KNU-141112 또는 백신주에 대한 중화활성을 검사하였다.
도 3에 나타난 바와 같이, 기니피그 항혈청은 매우 KNU-141112 감염의 억제에 매우 효과적이였으며, 평균 중화항체 (neutralizing antibody, NA) 역가 1:112였다. 반면 상대적으로 낮은 희석의 항혈청은 SM98-1 감염으로부터 Vero 세포를 완전히 방어하였으며, 평균 NA 역가는 1:37 였다. 대조적으로, 전면역 또는 면역조치되지 않은 혈청은 각 주에 대한 중화활성을 나타낸다.
종합하였을 때, 이러한 데이터는 분리한 KNU-141112가 면역조치된 동물에서 잠재적 항체 반응을 이끌어낼수 있음을 나타낸다. 그러나, 항혈청이 동종 야외 분리주를 강하게 인식하는 반면, 이종 PEDV 백신주에는 효과가 없었으며, 이는 백신주 및 야외 PEDVs간의 항원변이를 암시한다.
2.3. KNU - 141112감염의 병원성
4마리의 돼지를 KNU-141112 바이러스로 경구 챌린지 (challenge)하고, 2마리 대조군 동물을 배도 배양 배지로 접종하였다. 2마리의 돼지를 직접 접촉 노출을 위하여 동일한 방에 접종된 돼지와 함께 사육하였다. 임상적 신호를 매일 기록하고, 분변 면봉을 본 실험기간 동안 접종 전후로 수집하였다. 순응 기간 후, 모든 돼지는 활동적이며 정상 분변 농도를 나타내었다. PEDV-챌린지된 돼지는 1 DPI(day post-inoculation)에서 무기력 및 설사변을 포함하는 임상적 신호를 나타내었으며, 심각한 수양성 설사 및 이후 구토를 나타내었다. PEDV-관련 폐사가 1DPI에 접종된 돼지로부터 발생하였다. 접종된군과 함께 사육된 접촉 돼지는 설사변 및 구토를 2 DPI에 나타내었으며, 임상적 신호의 진행은 접종된 동물과 유사하였다. 또한, RT-PCR로 결정한 바와 같이, 1 또는 2 DPI에서 모든 접종된 및 접촉된 돼지는 PEDV에 양성이였으며, 각각 18.7 (범위 16.4-21.0) 및 20.1 (범위 15.4-23.1)의 평균 Ct 값으로 분변 내에 PEDV를 배설하였다. 음성 대조군 돼지의 경우 정상적인 분변과 함께 활발하였으며, 실험 기간동안 PEDV가 존재하는 분변은 검출되지 않았다.
1마리의 돼지가 PEDV로 폐사하였으며, 뒤이어 1 DPI에 검시를 실시하였으며, 나머지 접종된 돼지는 이후 검시를 위하여 임의로 선별하였다. 모든 접촉 및 대조군 동물은 사후검시연구를 위하여 안락사하였다. 모든 접종 또는 직접 접촉 (naive contact) 돼지는 거시적으로 전형적인 PED-유사 병변을 나타내었다. 소장이 팽창되고, 황색 유채액이 축적되어 있으며, 융모 위축에 의하여 벽은 얇고 투명하였다 (도 4, 패널 A). 위도 팽창되어 있으며, 멍울지고 소화되지 않은 유액으로 차있었다. 대조적으로, 다른 장내기관은 정상적이었다. 바이러스성장염과 일치하는 장의 미시적 관찰은 모든 접종된 및 접촉된 돼지에서 발달되어 있으며, 이는 소장의 장세포의 액포 및 소장융모의 축소 및 융합을 포함한다 (Fig 4, 패널 B 및 C). 유사한 미세 병변이 4 DPI까지 챌린지된 및 접촉된 돼지에서 지속적으로 관찰되었다. 더욱이, IFA 및 IHC 염색은 소장의 모든 조각내 위축된 융모 상의 상피 세포의 세포질에서 바이러스성 항원이 뚜렷하게 검출됨을 나타내었다 (도 4, 패널 D 내지 F). 이러한 결과는 자연적 또는 실험적으로 악성 PEDV주가 감염된 돼지의 것과 비교가능하다 (Debouck et al., 1981; Jung et al., 2014; Madson et al., 2014; Stevenson et al, 2013). 실험기간 동안 음성 대조군 돼지에서는 미시적 또는 거시적 장내 병변이 관찰되지 않았다.
2.3. KNU -141112의 완전 게놈의 특정
원형의 분변 샘플 (KNU-141112-분변) 및 세포 배양-계대된 KNU-141112-P5 및 -P10 바이러스의 전체 게놈 서열 데이터를 NGS 기술을 이용하여 결정하였다.
KOR/KNU-1305/2013 PEDV의 전장 게놈을 각 NGS 판독물에 대한 초기 참조 (initial reference)로서 사용하였으며, 개별적인 완전 게놈 서열을 각각의 NGS 판독물의 취합으로 성공적으로 수득하였다. 또한, 이러한 게놈의 5'및 3'말단을 RACE로 시퀀싱하였다. 3개의 모든 게놈은 3'poly(A) tail을 제외하고는 길이상 28,038 뉴클레오티드였으며, 292-nt 5' UTR, 20345-nt ORF1a/1b (1a 에 대하여 nt 293 내지 12601, 및 1b에 대하여 nt 12601 내지 20637), 4161-nt S 유전자 (nt 20634 내지 24794), 675-nt ORF3 (nt 24794 내지 25468), 231-nt E 유전자 (nt 25449 내지 25679), 681-nt 세포막 (membrane, M) 유전자 (nt 25687 내지 26367), 1326-nt N 유전자 (nt 26379 내지 27704), 및 334-nt 3' UTR로 이루어진, 종래 시퀀싱된 PEDV주의 전형적인 게놈 구조를 나타내었다. 슬리퍼리 헵타머 (slippery heptamer) 서열, TTTAAAC, 뒤이은 stem-loop 구조가 PEDV 게놈내의 3' 말단의 ORF1a에 나타났으며, 이는 pp 1ab를 생성하기 위한 번역 중 리보솜 프레임 시프트의 잠재적 신호에 해당한다.
3개의 모든 바이러스의 완전 개놈 서열을 비교하였으며, 그 결과를 하기 표 2에 나타내었다.
Figure pat00001
KNU-141112-feces, KNU-141112-P5의 비교는 21756 위치의 1개의 상이한 nt를 나타내며, 이는 S 단백질 내의 1개의 아미노산 (amino acid, aa)의 변화 (Leu 에서 Phe)를 야기한다. 반면, KNU-141112-P10은 21448 및 24492의 2개의 추가적 nt 변화를 획득하였으며, 이는 S 단백질 상 2개의 aa 돌연변이를 야기한다.
원형의 분변 및 선택된 패시지 (P3, P4, P5, P10, P20, 및 P30)의 KNU-141112 바이러스의 전장 S 유전자를 종래 시퀀싱 방법을 이용하여 시퀀싱하였다.
KNU-141112-feces, -P5, 및 -P10의 S 단백질 서열은 NGS로 결정한 서열과 완전히 일치하였다. KNU-141112-P5의 S-유전자에 나타난 한 개의 nt 변화는 패시지 3 (KNU-141112-P3)부터 발생하였다. 분변과 비교하여 10 패시지에서 확인된, 총 3개의 nt 돌연변이는 패시지 30 (KNU-141112-30)까지 지속되었다.
또한, KNU-141112-P10의 비교에서, KNU-141112-P20에서는 24869 및 25656 위치에서 2개의 독립적인 돌연변이를 검출할 수 있었으며, 이는 각각 ORF3 (Asp에서 Tyr) 및 E (Pro에서 Ser)의 아미노산 변화를 야기하며, 이는 패시지 30까지 유지되었다.
종합하여 볼 때, 이러한 결과는 PEDV 분리된 KNU-141112가 세포 계대 배양시 유전적으로 안정하다는 것을 나타낸다.
본 발명에서 결정된 PEDV의 전체 게놈 서열을 Genebank에서 확인 가능한 종래 3개의 다른 국내주 또는 8개의 국외 PEDV주와 비교하였으며, 이러한 뉴클레오티드 상동성 데이터를 하기 표 3에 나타내었다.
Figure pat00002
또한, 본 발명에서 결정된 PEDV의 스파이크 단백질에 대한 종래 Genebank에서 확인 가능한 종래 3개의 다른 국내주 또는 8개의 국외 PEDV주와 비교하였으며, 이러한 뉴클레오티드 상동성 데이터를 하기 표 4에 나타내었다.
Figure pat00003
KNU-141112 분리주 (feces, 4 P5, 및 P10)는 국내 재출현한 바이러스주 KOR-KNU-1305 및 US주인, IN17846 및 MN와 게놈 수준에서 8 내지 46개의 nt 차이를 나타내어, 가장 높은 뉴클레오티드 상동성 (99.9%)를 나타내었다.
3개의 모든 바이러스는 한국 백신주인 SM98-1 및 DR-13, 및 원형 CV777주와 유전적으로 분리되며, 96.3% 내지 96.8%의 범위로 비교적 낮은 뉴클레오티드 상동성을 나타내었다.
전세계적 PEDV주의 얼라인먼트에 따라, 20204와 20205간의 단일-뉴클레오티드 삽입이 1개의 중국 AH2012주 및 3개의 US 주에서 종래 확인되었으며, 이는 길이에서 pp 1ab 단백질의 단축을 야기한다 (Chen et al., 2014). 그러나, 3개의 PEDV의 개놈 모두 이러한 삽입을 포함하지 않으며, 이러한 결과를 Sanger 시퀀싱 방법에 의하여 확인하였다.
S 유전자 및 S1 부분이 상이한 PEDV 분리주 간의 유전적 관련성을 분석하기에 적절한 부위인 바, 계통발생 분석을 위하여 공개된 PEDV주의 양 부분를 사용하였다. 종래 결과도 동일하게 (Lee et al., 2010; Lee and Lee, 2014; Lee et al., 2014a; Lee et al., 2014b), 전장 S 유전자-기반 계통수는 전세계적 PEDV주가 2개의 클러스터로 명확하게 구분됨을 나타내었으며, 이를 genogroup 1 (G1; classical) 및 genogroup 2 (G2; field epidemic)으로 명명하였다. 각 genogroup은 subgroup 1a, 1b, 2a, 및 2b으로 다시 분류될 수 있다 (Fig. 5A). 모든 원형 분변 및 30 패시지까지 계대된 PEDV 바이러스는 최근 국내 야외 분리주와 마찬가지로 모두 subgroup 2b로 분류되었으며, 동일한 subgroupso의 인접한 계통군 내 신생 US주와 가장 가깝게 클러스터되었다. S1 부분을 이용한 이후의 계통발생분석은 S 유전자-기반 계통수와 동일한 그루핑 구조를 나타내었다.
또한, 전체 게놈 서열을 바탕으로 한 계통발생 분석은 KNU-141112가 최근 국내주 및 US주와 동일한 클러스터로 분류됨을 증명하였다 (도 5B).
한국생명공학연구원 KCTC12736BP 20141218
<110> Kyungpook National University Industry-Academic Cooperation Foundation <120> Korean porcine epidemic diarrhea virus isolate and use thereof <130> 1-250-1P <150> 10-2014-0184981 <151> 2014-12-19 <160> 45 <170> KopatentIn 2.0 <210> 1 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces spike protein <400> 1 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 2 <211> 6781 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces ORF1a/1b <400> 2 Met Ala Ser Asn His Val Thr Leu Ala Phe Ala Asn Asp Ala Glu Ile 1 5 10 15 Ser Ala Phe Gly Phe Cys Thr Ala Ser Glu Ala Val Ser Tyr Tyr Ser 20 25 30 Glu Ala Ala Ala Ser Gly Phe Met Gln Cys Arg Phe Val Ser Phe Asp 35 40 45 Leu Ala Asp Thr Val Glu Gly Leu Leu Pro Glu Asp Tyr Val Met Val 50 55 60 Val Val Gly Thr Thr Lys Leu Ser Ala Tyr Val Asp Thr Phe Gly Ser 65 70 75 80 Arg Pro Lys Asn Ile Cys Gly Trp Leu Leu Phe Ser Asn Cys Asn Tyr 85 90 95 Phe Leu Glu Glu Leu Glu Leu Thr Phe Gly Arg Arg Gly Gly Asn Ile 100 105 110 Val Pro Val Asp Gln Tyr Met Cys Gly Ala Asp Gly Lys Pro Val Leu 115 120 125 Gln Glu Ser Glu Trp Glu Tyr Thr Asp Phe Phe Ala Asp Ser Glu Asp 130 135 140 Gly Gln Leu Asn Ile Ala Gly Ile Thr Tyr Val Lys Ala Trp Ile Val 145 150 155 160 Glu Arg Ser Asp Val Ser Tyr Ala Ser Gln Asn Leu Thr Ser Ile Lys 165 170 175 Ser Ile Thr Tyr Cys Ser Thr Tyr Glu His Thr Phe Pro Asp Gly Thr 180 185 190 Ala Met Lys Val Ala Arg Thr Pro Lys Ile Lys Lys Thr Val Val Leu 195 200 205 Ser Glu Pro Leu Ala Thr Ile Tyr Arg Glu Ile Gly Ser Pro Phe Val 210 215 220 Asp Asn Gly Ser Asp Ala Arg Ser Ile Ile Lys Arg Pro Val Phe Leu 225 230 235 240 His Ala Phe Val Lys Cys Lys Cys Gly Ser Tyr His Trp Thr Val Gly 245 250 255 Asp Trp Thr Ser Tyr Val Ser Thr Cys Cys Gly Phe Lys Cys Lys Pro 260 265 270 Val Leu Val Ala Ser Cys Ser Ala Thr Pro Gly Ser Val Val Val Thr 275 280 285 Arg Ala Gly Ala Gly Thr Gly Val Lys Tyr Tyr Asn Asn Met Phe Leu 290 295 300 Arg His Val Ala Asp Ile Asp Gly Leu Ala Phe Trp Arg Ile Leu Lys 305 310 315 320 Val Gln Ser Lys Asp Asp Leu Ala Cys Ser Gly Lys Phe Leu Glu His 325 330 335 His Glu Glu Gly Phe Thr Asp Pro Cys Tyr Phe Leu Asn Asp Ser Ser 340 345 350 Ile Ala Thr Lys Leu Lys Phe Asp Ile Leu Ser Gly Lys Phe Ser Asp 355 360 365 Glu Val Lys Gln Ala Ile Phe Ala Gly His Val Val Val Gly Ser Ala 370 375 380 Leu Val Asp Ile Val Asp Asp Ala Leu Gly Gln Pro Trp Phe Ile Arg 385 390 395 400 Lys Leu Gly Asp Leu Ala Ser Ala Ala Trp Glu Gln Leu Lys Ala Val 405 410 415 Val Arg Gly Leu Asn Leu Leu Ser Asp Glu Val Val Leu Phe Gly Lys 420 425 430 Arg Leu Ser Cys Ala Thr Leu Ser Ile Val Asn Gly Val Phe Glu Phe 435 440 445 Ile Ala Glu Val Pro Glu Lys Leu Ala Ala Ala Val Thr Val Phe Val 450 455 460 Asn Phe Leu Asn Glu Leu Phe Glu Ser Ala Cys Asp Cys Leu Lys Val 465 470 475 480 Gly Gly Lys Thr Phe Asn Lys Val Gly Ser Tyr Val Leu Phe Asp Asn 485 490 495 Ala Leu Val Lys Leu Val Lys Ala Lys Val Arg Gly Pro Arg Gln Ala 500 505 510 Gly Val Cys Glu Val Arg Tyr Thr Ser Leu Val Ile Gly Ser Thr Thr 515 520 525 Lys Val Val Ser Lys Arg Val Glu Asn Ala Asn Val Asn Leu Val Val 530 535 540 Val Asp Glu Asp Val Thr Leu Asn Thr Thr Gly Arg Thr Val Val Val 545 550 555 560 Asp Gly Leu Ala Phe Phe Glu Ser Asp Gly Phe Tyr Arg His Leu Ala 565 570 575 Asp Ala Asp Val Val Ile Glu His Pro Val Tyr Lys Ser Ala Cys Glu 580 585 590 Leu Lys Pro Val Phe Glu Cys Asp Pro Ile Pro Asp Phe Pro Met Pro 595 600 605 Val Ala Ala Ser Val Ala Glu Leu Cys Val Gln Thr Asp Leu Leu Leu 610 615 620 Lys Asn Tyr Asn Thr Pro Tyr Lys Thr Tyr Ser Cys Val Val Arg Gly 625 630 635 640 Asp Lys Cys Cys Ile Thr Cys Thr Leu His Ile Thr Ala Pro Ser Tyr 645 650 655 Met Glu Asp Ala Ala Asn Phe Val Asp Leu Cys Thr Lys Asn Ile Gly 660 665 670 Thr Ala Gly Phe His Glu Phe Tyr Ile Thr Ala His Glu Gln Gln Asp 675 680 685 Leu Gln Gly Phe Val Thr Thr Cys Cys Thr Met Ser Gly Phe Glu Cys 690 695 700 Phe Met Pro Ile Ile Pro Gln Cys Pro Ala Val Leu Glu Glu Ile Asp 705 710 715 720 Gly Gly Ser Ile Trp Arg Ser Phe Ile Thr Gly Leu Asn Thr Met Trp 725 730 735 Asp Phe Cys Lys His Leu Lys Val Ser Phe Gly Leu Asp Gly Ile Val 740 745 750 Val Thr Val Ala Arg Lys Phe Lys Arg Leu Gly Ala Leu Leu Ala Glu 755 760 765 Met Tyr Asn Thr Tyr Leu Ser Thr Val Val Glu Asn Leu Val Leu Ala 770 775 780 Gly Val Ser Phe Lys Tyr Tyr Ala Thr Ser Val Pro Lys Ile Val Leu 785 790 795 800 Gly Cys Cys Phe His Ser Val Lys Ser Val Leu Ala Ser Ala Phe Gln 805 810 815 Ile Pro Val Gln Ala Gly Ile Glu Lys Phe Lys Val Phe Leu Asn Cys 820 825 830 Val His Pro Val Val Pro Arg Val Ile Glu Thr Ser Phe Val Glu Leu 835 840 845 Glu Glu Thr Thr Phe Lys Pro Pro Ala Leu Asn Gly Ser Ile Ala Ile 850 855 860 Val Asp Gly Phe Ala Phe Tyr Tyr Asp Gly Thr Leu Tyr Tyr Pro Thr 865 870 875 880 Asp Gly Asn Ser Val Val Pro Ile Cys Phe Lys Lys Lys Gly Gly Gly 885 890 895 Asp Val Lys Phe Ser Asp Glu Val Ser Val Arg Thr Ile Asp Pro Val 900 905 910 Tyr Lys Val Ser Leu Glu Phe Glu Phe Glu Ser Glu Thr Ile Met Ala 915 920 925 Val Leu Asn Lys Ala Val Gly Asn Arg Ile Lys Val Thr Gly Gly Trp 930 935 940 Asp Asp Val Val Glu Tyr Ile Asn Val Ala Ile Glu Val Leu Lys Asp 945 950 955 960 His Ile Asp Val Pro Lys Tyr Tyr Ile Tyr Asp Glu Glu Gly Gly Thr 965 970 975 Asp Pro Asn Leu Pro Val Met Val Ser Gln Trp Pro Leu Asn Asp Asp 980 985 990 Thr Ile Ser Gln Asp Leu Leu Asp Val Glu Val Val Thr Asp Ala Pro 995 1000 1005 Ile Asp Phe Glu Gly Asp Glu Val Asp Ser Ser Asp Pro Asp Lys Val 1010 1015 1020 Ala Asp Val Ala Asn Ser Glu Pro Glu Asp Asp Gly Pro Asn Val Ala 1025 1030 1035 1040 Pro Glu Thr Asn Val Glu Ser Glu Val Glu Glu Val Ala Ala Thr Leu 1045 1050 1055 Ser Phe Ile Lys Asp Thr Pro Ser Thr Val Thr Lys Asp Pro Phe Ala 1060 1065 1070 Phe Asp Phe Ala Ser Tyr Gly Gly Leu Lys Val Leu Arg Gln Ser His 1075 1080 1085 Asn Asn Cys Trp Val Thr Ser Thr Leu Val Gln Leu Gln Leu Leu Gly 1090 1095 1100 Ile Val Asp Asp Pro Ala Met Glu Leu Phe Ser Ala Gly Arg Val Gly 1105 1110 1115 1120 Pro Met Val Arg Lys Cys Tyr Glu Ser Gln Lys Ala Ile Leu Gly Ser 1125 1130 1135 Leu Gly Asp Val Ser Ala Cys Leu Glu Ser Leu Thr Lys Asp Leu His 1140 1145 1150 Thr Leu Lys Ile Thr Cys Ser Val Val Cys Gly Cys Gly Thr Gly Glu 1155 1160 1165 Arg Ile Tyr Glu Gly Cys Ala Phe Arg Met Thr Pro Thr Leu Glu Pro 1170 1175 1180 Phe Pro Tyr Gly Ala Cys Ala Gln Cys Ala Gln Val Leu Met His Thr 1185 1190 1195 1200 Phe Lys Ser Ile Val Gly Thr Gly Ile Phe Cys Arg Asp Thr Thr Ala 1205 1210 1215 Leu Ser Leu Asp Ser Leu Val Val Lys Pro Leu Cys Ala Ala Ala Phe 1220 1225 1230 Ile Gly Lys Asp Ser Gly His Tyr Val Thr Asn Phe Tyr Asp Ala Ala 1235 1240 1245 Met Ala Ile Asp Gly Tyr Gly Arg His Gln Ile Lys Tyr Asp Thr Leu 1250 1255 1260 Asn Thr Ile Cys Val Lys Asp Val Asn Trp Thr Ala Pro Phe Val Pro 1265 1270 1275 1280 Asp Val Glu Pro Val Leu Glu Pro Val Val Lys Pro Phe Tyr Ser Tyr 1285 1290 1295 Lys Asn Val Asp Phe Tyr Gln Gly Asp Phe Ser Asp Leu Val Lys Leu 1300 1305 1310 Pro Cys Asp Phe Val Val Asn Ala Ala Asn Glu Asn Leu Ser His Gly 1315 1320 1325 Gly Gly Ile Ala Lys Ala Ile Asp Val Tyr Thr Lys Gly Met Leu Gln 1330 1335 1340 Lys Cys Ser Asn Asp Tyr Ile Lys Ala His Gly Pro Ile Lys Val Gly 1345 1350 1355 1360 Arg Gly Val Met Leu Glu Ala Leu Gly Leu Lys Val Phe Asn Val Val 1365 1370 1375 Gly Pro Arg Lys Gly Lys His Ala Pro Glu Leu Leu Val Lys Ala Tyr 1380 1385 1390 Lys Ser Val Phe Ala Asn Ser Gly Val Ala Leu Thr Pro Leu Ile Ser 1395 1400 1405 Val Gly Ile Phe Ser Val Pro Leu Glu Glu Ser Leu Ser Ala Phe Leu 1410 1415 1420 Ala Cys Val Gly Asp Arg His Cys Lys Cys Phe Cys Tyr Ser Asp Lys 1425 1430 1435 1440 Glu Arg Glu Ala Ile Ile Asn Tyr Met Asp Gly Leu Val Asp Ala Ile 1445 1450 1455 Phe Lys Asp Ala Leu Val Asp Thr Thr Pro Val Gln Glu Asp Val Gln 1460 1465 1470 Gln Val Ser Gln Lys Pro Val Leu Pro Asn Phe Glu Pro Phe Arg Ile 1475 1480 1485 Glu Gly Ala His Ala Phe Tyr Glu Cys Asn Pro Glu Gly Leu Met Ser 1490 1495 1500 Leu Gly Ala Asp Lys Leu Val Leu Phe Thr Asn Ser Asn Leu Asp Phe 1505 1510 1515 1520 Cys Ser Val Gly Lys Cys Leu Asn Asn Val Thr Gly Gly Ala Leu Leu 1525 1530 1535 Glu Ala Ile Asn Val Phe Lys Lys Ser Asn Lys Thr Val Pro Ala Gly 1540 1545 1550 Asn Cys Val Thr Phe Glu Cys Ala Asp Met Ile Ser Ile Thr Met Val 1555 1560 1565 Val Leu Pro Ser Asp Gly Asp Ala Asn Tyr Asp Lys Asn Tyr Ala Arg 1570 1575 1580 Ala Val Val Lys Val Ser Lys Leu Lys Gly Lys Leu Leu Leu Ala Val 1585 1590 1595 1600 Gly Asp Ala Met Leu Tyr Ser Lys Leu Ser His Leu Ser Val Leu Gly 1605 1610 1615 Phe Val Ser Thr Pro Asp Asp Val Glu Arg Phe Tyr Ala Asn Lys Ser 1620 1625 1630 Val Val Ile Lys Val Thr Glu Asp Thr Arg Ser Val Lys Thr Val Lys 1635 1640 1645 Val Glu Ser Thr Val Thr Tyr Gly Gln Gln Ile Gly Pro Cys Leu Val 1650 1655 1660 Asn Asp Thr Val Val Thr Asp Asn Lys Pro Val Val Ala Asp Val Val 1665 1670 1675 1680 Ala Lys Val Val Pro Ser Ala Asn Trp Asp Ser His Tyr Gly Phe Asp 1685 1690 1695 Lys Ala Gly Glu Phe His Met Leu Asp His Thr Gly Phe Ala Phe Pro 1700 1705 1710 Ser Glu Val Val Asn Gly Arg Arg Val Leu Lys Thr Thr Asp Asn Asn 1715 1720 1725 Cys Trp Val Asn Val Thr Cys Leu Gln Leu Gln Phe Ala Arg Phe Arg 1730 1735 1740 Phe Lys Ser Ala Gly Leu Gln Ala Met Trp Glu Ser Tyr Cys Thr Gly 1745 1750 1755 1760 Asp Val Ala Met Phe Val His Trp Leu Tyr Trp Leu Thr Gly Val Asp 1765 1770 1775 Lys Gly Gln Pro Ser Asp Ser Glu Asn Ala Leu Asn Met Leu Ser Lys 1780 1785 1790 Tyr Ile Val Pro Ala Gly Ser Val Thr Ile Glu Arg Val Thr His Asp 1795 1800 1805 Gly Cys Cys Cys Ser Lys Arg Val Val Thr Ala Pro Val Val Asn Ala 1810 1815 1820 Ser Val Leu Lys Leu Gly Val Glu Asp Gly Leu Cys Pro His Gly Leu 1825 1830 1835 1840 Asn Tyr Ile Asp Lys Val Val Val Val Lys Gly Thr Thr Ile Val Val 1845 1850 1855 Asn Val Gly Lys Pro Val Val Ala Pro Ser His Leu Phe Leu Lys Gly 1860 1865 1870 Val Ser Tyr Thr Thr Phe Leu Asp Asn Gly Asn Gly Val Ala Gly His 1875 1880 1885 Tyr Thr Val Phe Asp His Asp Thr Gly Met Val His Asp Gly Asp Val 1890 1895 1900 Phe Val Pro Gly Asp Leu Asn Val Ser Pro Val Thr Asn Val Val Val 1905 1910 1915 1920 Ser Glu Gln Thr Ala Val Val Ile Lys Asp Pro Val Lys Lys Val Glu 1925 1930 1935 Leu Asp Ala Thr Lys Leu Leu Asp Thr Met Asn Tyr Ala Ser Glu Arg 1940 1945 1950 Phe Phe Ser Phe Gly Asp Phe Met Ser Arg Asn Leu Ile Thr Val Phe 1955 1960 1965 Leu Tyr Ile Leu Ser Ile Leu Gly Leu Cys Phe Arg Ala Phe Arg Lys 1970 1975 1980 Arg Asp Val Lys Val Leu Ala Gly Val Pro Gln Arg Thr Gly Ile Ile 1985 1990 1995 2000 Leu Arg Lys Ser Val Arg Tyr Asn Ala Lys Ala Leu Gly Val Phe Phe 2005 2010 2015 Lys Leu Lys Leu Tyr Trp Phe Lys Val Leu Gly Lys Phe Ser Leu Gly 2020 2025 2030 Ile Tyr Ala Leu Tyr Ala Leu Leu Phe Met Thr Ile Arg Phe Thr Pro 2035 2040 2045 Ile Gly Gly Pro Val Cys Asp Asp Val Val Ala Gly Tyr Ala Asn Ser 2050 2055 2060 Ser Phe Asp Lys Asn Glu Tyr Cys Asn Ser Val Ile Cys Lys Val Cys 2065 2070 2075 2080 Leu Tyr Gly Tyr Gln Glu Leu Ser Asp Phe Ser His Thr Gln Val Val 2085 2090 2095 Trp Gln His Leu Arg Asp Pro Leu Ile Gly Asn Val Met Pro Phe Phe 2100 2105 2110 Tyr Leu Ala Phe Leu Ala Ile Phe Gly Gly Val Tyr Val Lys Ala Ile 2115 2120 2125 Thr Leu Tyr Phe Ile Phe Gln Tyr Leu Asn Ile Leu Gly Val Phe Leu 2130 2135 2140 Gly Leu Gln Gln Ser Ile Trp Phe Leu Gln Leu Val Pro Phe Asp Val 2145 2150 2155 2160 Phe Gly Asp Glu Ile Val Val Phe Phe Ile Val Thr Arg Val Leu Met 2165 2170 2175 Phe Leu Lys His Val Phe Leu Gly Cys Asp Lys Ala Ser Cys Val Ala 2180 2185 2190 Cys Ser Lys Ser Ala Arg Leu Lys Arg Val Pro Val Gln Thr Ile Phe 2195 2200 2205 Gln Gly Thr Ser Lys Ser Phe Tyr Val His Ala Asn Gly Gly Ser Lys 2210 2215 2220 Phe Cys Lys Lys His Asn Phe Phe Cys Leu Asn Cys Asp Ser Tyr Gly 2225 2230 2235 2240 Pro Gly Cys Thr Phe Ile Asn Asp Val Ile Ala Thr Glu Val Gly Asn 2245 2250 2255 Val Val Lys Leu Asn Val Gln Pro Thr Gly Pro Ala Thr Ile Leu Ile 2260 2265 2270 Asp Lys Val Glu Phe Ser Asn Gly Phe Tyr Tyr Leu Tyr Ser Gly Asp 2275 2280 2285 Thr Phe Trp Lys Tyr Asn Phe Asp Ile Thr Asp Asn Lys Tyr Thr Cys 2290 2295 2300 Lys Glu Ser Leu Lys Asn Cys Ser Ile Ile Thr Asp Phe Ile Val Phe 2305 2310 2315 2320 Asn Asn Asn Gly Ser Asn Val Asn Gln Val Lys Asn Ala Cys Val Tyr 2325 2330 2335 Phe Ser Gln Met Leu Cys Lys Pro Val Lys Leu Val Asp Ser Ala Leu 2340 2345 2350 Leu Ala Ser Leu Ser Val Asp Phe Gly Ala Ser Leu His Ser Ala Phe 2355 2360 2365 Val Ser Val Leu Ser Asn Ser Phe Gly Lys Asp Leu Ser Ser Cys Asn 2370 2375 2380 Asp Met Gln Asp Cys Lys Ser Thr Leu Gly Phe Asp Asp Val Pro Leu 2385 2390 2395 2400 Asp Thr Phe Asn Ala Ala Val Ala Glu Ala His Arg Tyr Asp Val Leu 2405 2410 2415 Leu Thr Asp Met Ser Phe Asn Asn Phe Thr Thr Ser Tyr Ala Lys Pro 2420 2425 2430 Glu Glu Lys Leu Pro Val His Asp Ile Ala Thr Cys Met Arg Val Gly 2435 2440 2445 Ala Lys Ile Val Asn His Asn Val Leu Val Lys Asp Ser Ile Pro Val 2450 2455 2460 Val Trp Leu Val Arg Asp Phe Ile Val Leu Ser Glu Glu Thr Arg Lys 2465 2470 2475 2480 Tyr Ile Ile Arg Thr Thr Lys Val Lys Gly Ile Thr Phe Met Leu Thr 2485 2490 2495 Phe Asn Asp Cys Arg Met His Thr Thr Ile Pro Thr Val Cys Ile Ala 2500 2505 2510 Asn Lys Lys Gly Ala Gly Leu Pro Ser Phe Ser Lys Val Lys Lys Phe 2515 2520 2525 Phe Trp Phe Leu Cys Leu Phe Ile Val Ala Val Phe Phe Ala Leu Ser 2530 2535 2540 Phe Phe Asp Phe Ser Thr Gln Val Ser Ser Asp Ser Asp Tyr Asp Phe 2545 2550 2555 2560 Lys Tyr Ile Glu Ser Gly Gln Leu Lys Thr Phe Asp Asn Pro Leu Ser 2565 2570 2575 Cys Val His Asn Val Phe Ser Asn Phe Asp Gln Trp His Asp Ala Lys 2580 2585 2590 Phe Gly Phe Thr Pro Val Asn Asn Pro Ser Cys Pro Ile Val Val Gly 2595 2600 2605 Val Ser Asp Glu Ala Arg Thr Val Pro Gly Ile Pro Ala Gly Val Tyr 2610 2615 2620 Leu Ala Gly Lys Thr Leu Val Phe Ala Ile Asn Thr Ile Phe Gly Thr 2625 2630 2635 2640 Ser Gly Leu Cys Phe Asp Ala Ser Gly Val Ala Asp Lys Gly Ala Cys 2645 2650 2655 Ile Phe Asn Ser Ala Cys Thr Thr Leu Ser Gly Leu Gly Gly Thr Ala 2660 2665 2670 Val Tyr Cys Tyr Lys Asn Gly Leu Val Glu Gly Ala Lys Leu Tyr Ser 2675 2680 2685 Glu Leu Ala Pro His Ser Tyr Tyr Lys Met Val Asp Gly Asn Ala Val 2690 2695 2700 Ser Leu Pro Glu Ile Ile Ser Arg Gly Phe Gly Ile Arg Thr Ile Arg 2705 2710 2715 2720 Thr Lys Ala Met Thr Tyr Cys Arg Val Gly Gln Cys Val Gln Ser Ala 2725 2730 2735 Glu Gly Val Cys Phe Gly Ala Asp Arg Phe Phe Val Tyr Asn Ala Glu 2740 2745 2750 Ser Gly Ser Asp Phe Val Cys Gly Thr Gly Leu Phe Thr Leu Leu Met 2755 2760 2765 Asn Val Ile Ser Val Phe Ser Lys Thr Val Pro Val Thr Val Leu Ser 2770 2775 2780 Gly Gln Ile Leu Phe Asn Cys Ile Ile Ala Phe Ala Ala Val Ala Val 2785 2790 2795 2800 Cys Phe Leu Phe Thr Lys Phe Lys Arg Met Phe Gly Asp Met Ser Val 2805 2810 2815 Gly Val Phe Thr Val Gly Ala Cys Thr Leu Leu Asn Asn Val Ser Tyr 2820 2825 2830 Ile Val Thr Gln Asn Thr Leu Gly Met Leu Gly Tyr Ala Thr Leu Tyr 2835 2840 2845 Phe Leu Cys Thr Lys Gly Val Arg Tyr Met Trp Ile Trp His Leu Gly 2850 2855 2860 Phe Leu Ile Ser Tyr Ile Leu Ile Ala Pro Trp Trp Val Leu Met Val 2865 2870 2875 2880 Tyr Ala Phe Ser Ala Ile Phe Glu Phe Met Pro Asn Leu Phe Lys Leu 2885 2890 2895 Lys Val Ser Thr Gln Leu Phe Glu Gly Asp Lys Phe Val Gly Ser Phe 2900 2905 2910 Glu Asn Ala Ala Ala Gly Thr Phe Val Leu Asp Met His Ala Tyr Glu 2915 2920 2925 Arg Leu Ala Asn Ser Ile Ser Thr Glu Lys Leu Arg Gln Tyr Ala Ser 2930 2935 2940 Thr Tyr Asn Lys Tyr Lys Tyr Tyr Ser Gly Ser Ala Ser Glu Ala Asp 2945 2950 2955 2960 Tyr Arg Leu Ala Cys Phe Ala His Leu Ala Lys Ala Met Met Asp Tyr 2965 2970 2975 Ala Ser Asn His Asn Asp Thr Leu Tyr Thr Pro Pro Thr Val Ser Tyr 2980 2985 2990 Asn Ser Thr Leu Gln Ala Gly Leu Arg Lys Met Ala Gln Pro Ser Gly 2995 3000 3005 Val Val Glu Lys Cys Ile Val Arg Val Cys Tyr Gly Asn Met Ala Leu 3010 3015 3020 Asn Gly Leu Trp Leu Gly Asp Thr Val Ile Cys Pro Arg His Val Ile 3025 3030 3035 3040 Ala Ser Ser Thr Thr Ser Thr Ile Asp Tyr Asp Tyr Ala Leu Ser Val 3045 3050 3055 Leu Arg Leu His Asn Phe Ser Ile Ser Ser Gly Asn Val Phe Leu Gly 3060 3065 3070 Val Val Gly Val Thr Met Arg Gly Ala Leu Leu Gln Ile Lys Val Asn 3075 3080 3085 Gln Asn Asn Val His Thr Pro Lys Tyr Thr Tyr Arg Thr Val Arg Pro 3090 3095 3100 Gly Glu Ser Phe Asn Ile Leu Ala Cys Tyr Asp Gly Ser Ala Ala Gly 3105 3110 3115 3120 Val Tyr Gly Val Asn Met Arg Ser Asn Tyr Thr Ile Arg Gly Ser Phe 3125 3130 3135 Ile Asn Gly Ala Cys Gly Ser Pro Gly Tyr Asn Ile Asn Asn Gly Thr 3140 3145 3150 Val Glu Phe Cys Tyr Leu His Gln Leu Glu Leu Gly Ser Gly Cys His 3155 3160 3165 Val Gly Ser Asp Leu Asp Gly Val Met Tyr Gly Gly Tyr Glu Asp Gln 3170 3175 3180 Pro Thr Leu Gln Val Glu Gly Ala Ser Ser Leu Phe Thr Glu Asn Val 3185 3190 3195 3200 Leu Ala Phe Leu Tyr Ala Ala Leu Ile Asn Gly Ser Thr Trp Trp Leu 3205 3210 3215 Ser Ser Ser Arg Ile Ala Val Asp Arg Phe Asn Glu Trp Ala Val His 3220 3225 3230 Asn Gly Met Thr Thr Val Val Asn Thr Asp Cys Phe Ser Ile Leu Ala 3235 3240 3245 Ala Lys Thr Gly Val Asp Val Gln Arg Leu Leu Ala Ser Ile Gln Ser 3250 3255 3260 Leu His Lys Asn Phe Gly Gly Lys Gln Ile Leu Gly Tyr Thr Ser Leu 3265 3270 3275 3280 Thr Asp Glu Phe Thr Thr Gly Glu Val Ile Arg Gln Met Tyr Gly Val 3285 3290 3295 Asn Leu Gln Ser Gly Tyr Val Ser Arg Ala Cys Arg Asn Val Leu Leu 3300 3305 3310 Val Gly Ser Phe Leu Thr Phe Phe Trp Ser Glu Leu Val Ser Tyr Thr 3315 3320 3325 Lys Phe Phe Trp Val Asn Pro Gly Tyr Val Thr Pro Met Phe Ala Cys 3330 3335 3340 Leu Ser Leu Leu Ser Ser Leu Leu Met Phe Thr Leu Lys His Lys Thr 3345 3350 3355 3360 Leu Phe Phe Gln Val Phe Leu Ile Pro Ala Leu Ile Val Thr Ser Cys 3365 3370 3375 Ile Asn Leu Ala Phe Asp Val Glu Val Tyr Asn Tyr Leu Ala Glu His 3380 3385 3390 Phe Asp Tyr His Val Ser Leu Met Gly Phe Asn Ala Gln Gly Leu Val 3395 3400 3405 Asn Ile Phe Val Cys Phe Val Val Thr Ile Leu His Gly Thr Tyr Thr 3410 3415 3420 Trp Arg Phe Phe Asn Thr Pro Val Ser Ser Val Thr Tyr Val Val Ala 3425 3430 3435 3440 Leu Leu Thr Ala Ala Tyr Asn Tyr Phe Tyr Ala Ser Asp Ile Leu Ser 3445 3450 3455 Cys Ala Met Thr Leu Phe Ala Ser Val Thr Gly Asn Trp Phe Val Gly 3460 3465 3470 Ala Val Cys Tyr Lys Ala Ala Val Tyr Met Ala Leu Arg Phe Pro Thr 3475 3480 3485 Phe Val Ala Ile Phe Gly Asp Ile Lys Ser Val Met Phe Cys Tyr Leu 3490 3495 3500 Val Leu Gly Tyr Phe Thr Cys Cys Phe Tyr Gly Ile Leu Tyr Trp Phe 3505 3510 3515 3520 Asn Arg Phe Phe Lys Val Ser Val Gly Val Tyr Asp Tyr Thr Val Ser 3525 3530 3535 Ala Ala Glu Phe Lys Tyr Met Val Ala Asn Gly Leu Arg Ala Pro Thr 3540 3545 3550 Gly Thr Leu Asp Ser Leu Leu Leu Ser Ala Lys Leu Ile Gly Ile Gly 3555 3560 3565 Gly Glu Arg Asn Ile Lys Ile Ser Ser Val Gln Ser Lys Leu Thr Asp 3570 3575 3580 Ile Lys Cys Ser Asn Val Val Leu Leu Gly Cys Leu Ser Ser Met Asn 3585 3590 3595 3600 Val Ser Ala Asn Ser Thr Glu Trp Ala Tyr Cys Val Asp Leu His Asn 3605 3610 3615 Lys Ile Asn Leu Cys Asn Asp Pro Glu Lys Ala Gln Glu Met Leu Leu 3620 3625 3630 Ala Leu Leu Ala Phe Phe Leu Ser Lys Asn Ser Ala Phe Gly Leu Asp 3635 3640 3645 Asp Leu Leu Glu Ser Tyr Phe Asn Asp Asn Ser Met Leu Gln Ser Val 3650 3655 3660 Ala Ser Thr Tyr Val Gly Leu Pro Ser Tyr Val Ile Tyr Glu Asn Ala 3665 3670 3675 3680 Arg Gln Gln Tyr Glu Asp Ala Val Asn Asn Gly Ser Pro Pro Gln Leu 3685 3690 3695 Val Lys Gln Leu Arg His Ala Met Asn Val Ala Lys Ser Glu Phe Asp 3700 3705 3710 Arg Glu Ala Ser Thr Gln Arg Lys Leu Asp Arg Met Ala Glu Gln Ala 3715 3720 3725 Ala Ala Gln Met Tyr Lys Glu Ala Arg Ala Val Asn Arg Lys Ser Lys 3730 3735 3740 Val Val Ser Ala Met His Ser Leu Leu Phe Gly Met Leu Arg Arg Leu 3745 3750 3755 3760 Asp Met Ser Ser Val Asp Thr Ile Leu Asn Leu Ala Lys Asp Gly Val 3765 3770 3775 Val Pro Leu Ser Val Ile Pro Ala Val Ser Ala Thr Lys Leu Asn Ile 3780 3785 3790 Val Thr Ser Asp Ile Asp Ser Tyr Asn Arg Ile Gln Arg Glu Gly Cys 3795 3800 3805 Val His Tyr Ala Gly Thr Ile Trp Asn Ile Ile Asp Ile Lys Asp Asn 3810 3815 3820 Asp Gly Lys Val Val His Val Lys Glu Val Thr Ala Gln Asn Ala Glu 3825 3830 3835 3840 Ser Leu Ser Trp Pro Leu Val Leu Gly Cys Glu Arg Ile Val Lys Leu 3845 3850 3855 Gln Asn Asn Glu Ile Ile Pro Gly Lys Leu Lys Gln Arg Ser Ile Lys 3860 3865 3870 Ala Glu Gly Asp Gly Ile Val Gly Glu Gly Lys Ala Leu Tyr Asn Asn 3875 3880 3885 Glu Gly Gly Arg Thr Phe Met Tyr Ala Phe Ile Ser Asp Lys Pro Asp 3890 3895 3900 Leu Arg Val Val Lys Trp Glu Phe Asp Gly Gly Cys Asn Thr Ile Glu 3905 3910 3915 3920 Leu Glu Pro Pro Arg Lys Phe Leu Val Asp Ser Pro Asn Gly Ala Gln 3925 3930 3935 Ile Lys Tyr Leu Tyr Phe Val Arg Asn Leu Asn Thr Leu Arg Arg Gly 3940 3945 3950 Ala Val Leu Gly Tyr Ile Gly Ala Thr Val Arg Leu Gln Ala Gly Lys 3955 3960 3965 Gln Thr Glu Gln Ala Ile Asn Ser Ser Leu Leu Thr Leu Cys Ala Phe 3970 3975 3980 Ala Val Asp Pro Ala Lys Thr Tyr Ile Asp Ala Val Lys Ser Gly His 3985 3990 3995 4000 Lys Pro Val Gly Asn Cys Val Lys Met Leu Ala Asn Gly Ser Gly Asn 4005 4010 4015 Gly Gln Ala Val Thr Asn Gly Val Glu Ala Ser Thr Asn Gln Asp Ser 4020 4025 4030 Tyr Gly Gly Ala Ser Val Cys Leu Tyr Cys Arg Ala His Val Glu His 4035 4040 4045 Pro Ser Met Asp Gly Phe Cys Arg Leu Lys Gly Lys Tyr Val Gln Val 4050 4055 4060 Pro Leu Gly Thr Val Asp Pro Ile Arg Phe Val Leu Glu Asn Asp Val 4065 4070 4075 4080 Cys Lys Val Cys Gly Cys Trp Leu Ala Asn Gly Cys Thr Cys Asp Arg 4085 4090 4095 Ser Ile Met Gln Ser Thr Asp Tyr Gly Leu Phe Lys Arg Val Arg Gly 4100 4105 4110 Ser Ser Ala Ala Arg Leu Glu Pro Cys Asn Gly Thr Asp Thr Gln His 4115 4120 4125 Val Tyr Arg Ala Phe Asp Ile Tyr Asn Lys Asp Val Ala Cys Leu Gly 4130 4135 4140 Lys Phe Leu Lys Val Asn Cys Val Arg Leu Lys Asn Leu Asp Lys His 4145 4150 4155 4160 Asp Ala Phe Tyr Val Val Lys Arg Cys Thr Lys Ser Ala Met Glu His 4165 4170 4175 Glu Gln Ser Ile Tyr Ser Arg Leu Glu Lys Cys Gly Ala Val Ala Glu 4180 4185 4190 His Asp Phe Phe Thr Trp Lys Asp Gly Arg Ala Ile Tyr Gly Asn Val 4195 4200 4205 Cys Arg Lys Asp Leu Thr Glu Tyr Thr Met Met Asp Leu Cys Tyr Ala 4210 4215 4220 Leu Arg Asn Phe Asp Glu Asn Asn Cys Asp Val Leu Lys Ser Ile Leu 4225 4230 4235 4240 Ile Lys Val Gly Ala Cys Glu Glu Ser Tyr Phe Asn Asn Lys Val Trp 4245 4250 4255 Phe Asp Pro Val Glu Asn Glu Asp Ile His Arg Val Tyr Ala Leu Leu 4260 4265 4270 Gly Thr Ile Val Ser Arg Ala Met Leu Lys Cys Val Lys Phe Cys Asp 4275 4280 4285 Ala Met Val Glu Gln Gly Ile Val Gly Val Val Thr Leu Asp Asn Gln 4290 4295 4300 Asp Leu Asn Gly Asp Phe Tyr Asp Phe Gly Asp Phe Thr Cys Ser Ile 4305 4310 4315 4320 Lys Gly Met Gly Ile Pro Ile Cys Thr Ser Tyr Tyr Ser Tyr Met Met 4325 4330 4335 Pro Val Met Gly Met Thr Asn Cys Leu Ala Ser Glu Cys Phe Val Lys 4340 4345 4350 Ser Asp Ile Phe Gly Glu Asp Phe Lys Ser Tyr Asp Leu Leu Glu Tyr 4355 4360 4365 Asp Phe Thr Glu His Lys Thr Ala Leu Phe Asn Lys Tyr Phe Lys Tyr 4370 4375 4380 Trp Gly Leu Gln Tyr His Pro Asn Cys Val Asp Cys Ser Asp Glu Gln 4385 4390 4395 4400 Cys Ile Val His Cys Ala Asn Phe Asn Thr Leu Phe Ser Thr Thr Ile 4405 4410 4415 Pro Ile Thr Ala Phe Gly Pro Leu Cys Arg Lys Cys Trp Ile Asp Gly 4420 4425 4430 Val Pro Leu Val Thr Thr Ala Gly Tyr His Phe Lys Gln Leu Gly Ile 4435 4440 4445 Val Trp Asn Asn Asp Leu Asn Leu His Ser Ser Arg Leu Ser Ile Asn 4450 4455 4460 Glu Leu Leu Gln Phe Cys Ser Asp Pro Ala Leu Leu Ile Ala Ser Ser 4465 4470 4475 4480 Pro Ala Leu Val Asp Gln Arg Thr Val Cys Phe Ser Val Ala Ala Leu 4485 4490 4495 Gly Thr Gly Met Thr Asn Gln Thr Val Lys Pro Gly His Phe Asn Lys 4500 4505 4510 Glu Phe Tyr Asp Phe Leu Leu Glu Gln Gly Phe Phe Ser Glu Gly Ser 4515 4520 4525 Glu Leu Thr Leu Lys His Phe Phe Phe Ala Gln Lys Gly Asp Ala Ala 4530 4535 4540 Val Lys Asp Phe Asp Tyr Tyr Arg Tyr Asn Arg Pro Thr Val Leu Asp 4545 4550 4555 4560 Ile Cys Gln Ala Arg Val Val Tyr Gln Ile Val Gln Arg Tyr Phe Asp 4565 4570 4575 Ile Tyr Glu Gly Gly Cys Ile Thr Ala Lys Glu Val Val Val Thr Asn 4580 4585 4590 Leu Asn Lys Ser Ala Gly Tyr Pro Leu Asn Lys Phe Gly Lys Ala Gly 4595 4600 4605 Leu Tyr Tyr Glu Ser Leu Ser Tyr Glu Glu Gln Asp Glu Leu Tyr Ala 4610 4615 4620 Tyr Thr Lys Arg Asn Ile Leu Pro Thr Met Thr Gln Leu Asn Leu Lys 4625 4630 4635 4640 Tyr Ala Ile Ser Gly Lys Glu Arg Ala Arg Thr Val Gly Gly Val Ser 4645 4650 4655 Leu Leu Ser Thr Met Thr Thr Arg Gln Tyr His Gln Lys His Leu Lys 4660 4665 4670 Ser Ile Val Asn Thr Arg Gly Ala Ser Val Val Ile Gly Thr Thr Lys 4675 4680 4685 Phe Tyr Gly Gly Trp Asp Asn Met Leu Lys Asn Leu Ile Asp Gly Val 4690 4695 4700 Glu Asn Pro Cys Leu Met Gly Trp Asp Tyr Pro Lys Cys Asp Arg Ala 4705 4710 4715 4720 Leu Pro Asn Met Ile Arg Met Ile Ser Ala Met Ile Leu Gly Ser Lys 4725 4730 4735 His Thr Thr Cys Cys Ser Ser Thr Asp Arg Phe Phe Arg Leu Cys Asn 4740 4745 4750 Glu Leu Ala Gln Val Leu Thr Glu Val Val Tyr Ser Asn Gly Gly Phe 4755 4760 4765 Tyr Leu Lys Pro Gly Gly Thr Thr Ser Gly Asp Ala Thr Thr Ala Tyr 4770 4775 4780 Ala Asn Ser Val Phe Asn Ile Phe Gln Ala Val Ser Ala Asn Val Asn 4785 4790 4795 4800 Lys Leu Leu Ser Val Asp Ser Asn Val Cys His Asn Leu Glu Val Lys 4805 4810 4815 Gln Leu Gln Arg Lys Leu Tyr Glu Cys Cys Tyr Arg Ser Thr Thr Val 4820 4825 4830 Asp Asp Gln Phe Val Val Glu Tyr Tyr Gly Tyr Leu Arg Lys His Phe 4835 4840 4845 Ser Met Met Ile Leu Ser Asp Asp Gly Val Val Cys Tyr Asn Asn Asp 4850 4855 4860 Tyr Ala Ser Leu Gly Tyr Val Ala Asp Leu Asn Ala Phe Lys Ala Val 4865 4870 4875 4880 Leu Tyr Tyr Gln Asn Asn Val Phe Met Ser Ala Ser Lys Cys Trp Ile 4885 4890 4895 Glu Pro Asp Ile Asn Lys Gly Pro His Glu Phe Cys Ser Gln His Thr 4900 4905 4910 Met Gln Ile Val Asp Lys Asp Gly Thr Tyr Tyr Leu Pro Tyr Pro Asp 4915 4920 4925 Pro Ser Arg Ile Leu Ser Ala Gly Val Phe Val Asp Asp Val Val Lys 4930 4935 4940 Thr Asp Ala Val Val Leu Leu Glu Arg Tyr Val Ser Leu Ala Ile Asp 4945 4950 4955 4960 Ala Tyr Pro Leu Ser Lys His Glu Asn Pro Glu Tyr Lys Lys Val Phe 4965 4970 4975 Tyr Val Leu Leu Asp Trp Val Lys His Leu Tyr Lys Thr Leu Asn Ala 4980 4985 4990 Gly Val Leu Glu Ser Phe Ser Val Thr Leu Leu Glu Asp Ser Thr Ala 4995 5000 5005 Lys Phe Trp Asp Glu Ser Phe Tyr Ala Asn Met Tyr Glu Lys Ser Ala 5010 5015 5020 Val Leu Gln Ser Ala Gly Leu Cys Val Val Cys Gly Ser Gln Thr Val 5025 5030 5035 5040 Leu Arg Cys Gly Asp Cys Leu Arg Arg Pro Met Leu Cys Thr Lys Cys 5045 5050 5055 Ala Tyr Asp His Val Ile Gly Thr Thr His Lys Phe Ile Leu Ala Ile 5060 5065 5070 Thr Pro Tyr Val Cys Cys Ala Ser Asp Cys Gly Val Asn Asp Val Thr 5075 5080 5085 Lys Leu Tyr Leu Gly Gly Leu Ser Tyr Trp Cys His Asp His Lys Pro 5090 5095 5100 Arg Leu Ala Phe Pro Leu Cys Ser Ala Gly Asn Val Phe Gly Leu Tyr 5105 5110 5115 5120 Lys Asn Ser Ala Thr Gly Ser Pro Asp Val Glu Asp Phe Asn Arg Ile 5125 5130 5135 Ala Thr Ser Asp Trp Thr Asp Val Ser Asp Tyr Arg Leu Ala Asn Asp 5140 5145 5150 Val Lys Asp Ser Leu Arg Leu Phe Ala Ala Glu Thr Ile Lys Ala Lys 5155 5160 5165 Glu Glu Ser Val Lys Ser Ser Tyr Ala Cys Ala Thr Leu His Glu Val 5170 5175 5180 Val Gly Pro Lys Glu Leu Leu Leu Lys Trp Glu Val Gly Arg Pro Lys 5185 5190 5195 5200 Pro Pro Leu Asn Arg Asn Ser Val Phe Thr Cys Tyr His Ile Thr Lys 5205 5210 5215 Asn Thr Lys Phe Gln Ile Gly Glu Phe Val Phe Glu Lys Ala Glu Tyr 5220 5225 5230 Asp Asn Asp Ala Val Thr Tyr Lys Thr Thr Ala Thr Thr Lys Leu Val 5235 5240 5245 Pro Gly Met Val Phe Val Leu Thr Ser His Asn Val Gln Pro Leu Arg 5250 5255 5260 Ala Pro Thr Ile Ala Asn Gln Glu Arg Tyr Ser Thr Ile His Lys Leu 5265 5270 5275 5280 His Pro Ala Phe Asn Ile Pro Glu Ala Tyr Ser Ser Leu Val Pro Tyr 5285 5290 5295 Tyr Gln Leu Ile Gly Lys Gln Lys Ile Thr Thr Ile Gln Gly Pro Pro 5300 5305 5310 Gly Ser Gly Lys Ser His Cys Val Ile Gly Leu Gly Leu Tyr Tyr Pro 5315 5320 5325 Gly Ala Arg Ile Val Phe Thr Ala Cys Ser His Ala Ala Val Asp Ser 5330 5335 5340 Leu Cys Val Lys Ala Ser Thr Ala Tyr Ser Asn Asp Lys Cys Ser Arg 5345 5350 5355 5360 Ile Ile Pro Gln Arg Ala Arg Val Glu Cys Tyr Asp Gly Phe Lys Ser 5365 5370 5375 Asn Asn Thr Ser Ala Gln Tyr Leu Phe Ser Thr Val Asn Ala Leu Pro 5380 5385 5390 Glu Cys Asn Ala Asp Ile Val Val Val Asp Glu Val Ser Met Cys Thr 5395 5400 5405 Asn Tyr Asp Leu Ser Val Ile Asn Gln Arg Ile Ser Tyr Arg His Val 5410 5415 5420 Val Tyr Val Gly Asp Pro Gln Gln Leu Pro Ala Pro Arg Val Met Ile 5425 5430 5435 5440 Ser Arg Gly Thr Leu Glu Pro Lys Asp Tyr Asn Val Val Thr Gln Arg 5445 5450 5455 Met Cys Ala Leu Lys Pro Asp Val Phe Leu His Lys Cys Tyr Arg Cys 5460 5465 5470 Pro Ala Glu Ile Val Arg Thr Val Ser Glu Met Val Tyr Glu Asn Gln 5475 5480 5485 Phe Ile Pro Val His Pro Asp Ser Lys Gln Cys Phe Lys Ile Phe Cys 5490 5495 5500 Lys Gly Asn Val Gln Val Asp Asn Gly Ser Ser Ile Asn Arg Arg Gln 5505 5510 5515 5520 Leu Asp Val Val Arg Met Phe Leu Ala Lys Asn Pro Arg Trp Ser Lys 5525 5530 5535 Ala Val Phe Ile Ser Pro Tyr Asn Ser Gln Asn Tyr Val Ala Ser Arg 5540 5545 5550 Met Leu Gly Leu Gln Ile Gln Thr Val Asp Ser Ser Gln Gly Ser Glu 5555 5560 5565 Tyr Asp Tyr Val Ile Tyr Thr Gln Thr Ser Asp Thr Ala His Ala Cys 5570 5575 5580 Asn Val Asn Arg Phe Asn Val Ala Ile Thr Arg Ala Lys Lys Gly Ile 5585 5590 5595 5600 Leu Cys Ile Met Cys Asp Arg Ser Leu Phe Asp Val Leu Lys Phe Phe 5605 5610 5615 Glu Leu Lys Leu Ser Asp Leu Gln Ala Asn Glu Gly Cys Gly Leu Phe 5620 5625 5630 Lys Asp Cys Ser Arg Gly Asp Asp Leu Leu Pro Pro Ser His Ala Asn 5635 5640 5645 Thr Phe Met Ser Leu Ala Asp Asn Phe Lys Thr Asp Gln Asp Leu Ala 5650 5655 5660 Val Gln Ile Gly Val Asn Gly Pro Ile Lys Tyr Glu His Val Ile Ser 5665 5670 5675 5680 Phe Met Gly Phe Arg Phe Asp Ile Asn Ile Pro Asn His His Thr Leu 5685 5690 5695 Phe Cys Thr Arg Asp Phe Ala Met Arg Asn Val Arg Gly Trp Leu Gly 5700 5705 5710 Phe Asp Val Glu Gly Ala His Val Val Gly Ser Asn Val Gly Thr Asn 5715 5720 5725 Val Pro Leu Gln Leu Gly Phe Ser Asn Gly Val Asp Phe Val Val Arg 5730 5735 5740 Pro Glu Gly Cys Val Val Thr Glu Ser Gly Asp Tyr Ile Lys Pro Val 5745 5750 5755 5760 Arg Ala Arg Ala Pro Pro Gly Glu Gln Phe Ala His Leu Leu Pro Leu 5765 5770 5775 Leu Lys Arg Gly Gln Pro Trp Asp Val Val Arg Lys Arg Ile Val Gln 5780 5785 5790 Met Cys Ser Asp Tyr Leu Ala Asn Leu Ser Asp Ile Leu Ile Phe Val 5795 5800 5805 Leu Trp Ala Gly Gly Leu Glu Leu Thr Thr Met Arg Tyr Phe Val Lys 5810 5815 5820 Ile Gly Pro Ser Lys Ser Cys Asp Cys Gly Lys Val Ala Thr Cys Tyr 5825 5830 5835 5840 Asn Ser Ala Leu His Thr Tyr Cys Cys Phe Lys His Ala Leu Gly Cys 5845 5850 5855 Asp Tyr Leu Tyr Asn Pro Tyr Cys Ile Asp Ile Gln Gln Trp Gly Tyr 5860 5865 5870 Lys Gly Ser Leu Ser Leu Asn His His Glu His Cys Asn Val His Arg 5875 5880 5885 Asn Glu His Val Ala Ser Gly Asp Ala Ile Met Thr Arg Cys Leu Ala 5890 5895 5900 Ile His Asp Cys Phe Val Lys Asn Val Asp Trp Ser Ile Thr Tyr Pro 5905 5910 5915 5920 Phe Ile Gly Asn Glu Ala Val Ile Asn Lys Ser Gly Arg Ile Val Gln 5925 5930 5935 Ser His Thr Met Arg Ser Val Leu Lys Leu Tyr Asn Pro Lys Ala Ile 5940 5945 5950 Tyr Asp Ile Gly Asn Pro Lys Gly Ile Arg Cys Ala Val Thr Asp Ala 5955 5960 5965 Lys Trp Phe Cys Phe Asp Lys Asn Pro Thr Asn Ser Asn Val Lys Thr 5970 5975 5980 Leu Glu Tyr Asp Tyr Ile Thr His Gly Gln Phe Asp Gly Leu Cys Leu 5985 5990 5995 6000 Phe Trp Asn Cys Asn Val Asp Met Tyr Pro Glu Phe Ser Val Val Cys 6005 6010 6015 Arg Phe Asp Thr Arg Cys Arg Ser Pro Leu Asn Leu Glu Gly Cys Asn 6020 6025 6030 Gly Gly Ser Leu Tyr Val Asn Asn His Ala Phe His Thr Pro Ala Phe 6035 6040 6045 Asp Lys Arg Ala Phe Ala Lys Leu Lys Pro Met Pro Phe Phe Phe Tyr 6050 6055 6060 Asp Asp Thr Glu Cys Asp Lys Leu Gln Asp Ser Ile Asn Tyr Val Pro 6065 6070 6075 6080 Leu Arg Ala Ser Asn Cys Ile Thr Lys Cys Asn Val Gly Gly Ala Val 6085 6090 6095 Cys Ser Lys His Cys Ala Met Tyr His Ser Tyr Val Asn Ala Tyr Asn 6100 6105 6110 Thr Phe Thr Ser Ala Gly Phe Thr Ile Trp Val Pro Thr Ser Phe Asp 6115 6120 6125 Thr Tyr Asn Leu Trp Gln Thr Phe Ser Asn Asn Leu Gln Gly Leu Glu 6130 6135 6140 Asn Ile Ala Phe Asn Val Val Lys Lys Gly Ser Phe Val Gly Ala Glu 6145 6150 6155 6160 Gly Glu Leu Pro Val Ala Val Val Asn Asp Lys Val Leu Val Arg Asp 6165 6170 6175 Gly Thr Val Asp Thr Leu Val Phe Thr Asn Lys Thr Ser Leu Pro Thr 6180 6185 6190 Asn Val Ala Phe Glu Leu Tyr Ala Lys Arg Lys Val Gly Leu Thr Pro 6195 6200 6205 Pro Ile Thr Ile Leu Arg Asn Leu Gly Val Val Cys Thr Ser Lys Cys 6210 6215 6220 Val Ile Trp Asp Tyr Glu Ala Glu Arg Pro Leu Thr Thr Phe Thr Lys 6225 6230 6235 6240 Asp Val Cys Lys Tyr Thr Asp Phe Glu Gly Asp Val Cys Thr Leu Phe 6245 6250 6255 Asp Asn Ser Ile Val Gly Ser Leu Glu Arg Phe Ser Met Thr Gln Asn 6260 6265 6270 Ala Val Leu Met Ser Leu Thr Ala Val Lys Lys Leu Thr Gly Ile Lys 6275 6280 6285 Leu Thr Tyr Gly Tyr Leu Asn Gly Val Pro Val Asn Thr His Glu Asp 6290 6295 6300 Lys Pro Phe Thr Trp Tyr Ile Tyr Thr Arg Lys Asn Gly Lys Phe Glu 6305 6310 6315 6320 Asp His Pro Asp Gly Tyr Phe Thr Gln Gly Arg Thr Thr Ala Asp Phe 6325 6330 6335 Ser Pro Arg Ser Asp Met Glu Lys Asp Phe Leu Ser Met Asp Met Gly 6340 6345 6350 Leu Phe Ile Asn Lys Tyr Gly Leu Glu Asp Tyr Gly Phe Glu His Val 6355 6360 6365 Val Tyr Gly Asp Val Ser Lys Thr Thr Leu Gly Gly Leu His Leu Leu 6370 6375 6380 Ile Ser Gln Val Arg Leu Ala Cys Met Gly Val Leu Lys Ile Asp Glu 6385 6390 6395 6400 Phe Val Ser Ser Asn Asp Ser Thr Leu Lys Ser Cys Thr Val Thr Tyr 6405 6410 6415 Ala Asp Asn Pro Ser Ser Lys Met Val Cys Thr Tyr Met Asp Leu Leu 6420 6425 6430 Leu Asp Asp Phe Val Ser Ile Leu Lys Ser Leu Asp Leu Gly Val Val 6435 6440 6445 Ser Lys Val His Glu Val Met Val Asp Cys Lys Met Trp Arg Trp Met 6450 6455 6460 Leu Trp Cys Lys Asp His Lys Leu Gln Thr Phe Tyr Pro Gln Leu Gln 6465 6470 6475 6480 Ala Ser Glu Trp Lys Cys Gly Tyr Ser Met Pro Ser Ile Tyr Lys Ile 6485 6490 6495 Gln Arg Met Cys Leu Glu Pro Cys Asn Leu Tyr Asn Tyr Gly Ala Gly 6500 6505 6510 Ile Lys Leu Pro Asp Gly Ile Met Phe Asn Val Val Lys Tyr Thr Gln 6515 6520 6525 Leu Cys Gln Tyr Leu Asn Ser Thr Thr Met Cys Val Pro His His Met 6530 6535 6540 Arg Val Leu His Leu Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly 6545 6550 6555 6560 Thr Ala Val Leu Arg Arg Trp Leu Pro Leu Asp Ala Ile Ile Val Asp 6565 6570 6575 Asn Asp Ser Val Asp Tyr Val Ser Asp Ala Asp Tyr Ser Val Thr Gly 6580 6585 6590 Asp Cys Ser Thr Leu Tyr Leu Ser Asp Lys Phe Asp Leu Val Ile Ser 6595 6600 6605 Asp Met Tyr Asp Gly Lys Ile Lys Ser Cys Asp Gly Glu Asn Val Ser 6610 6615 6620 Lys Glu Gly Phe Phe Pro Tyr Ile Asn Gly Val Ile Thr Glu Lys Leu 6625 6630 6635 6640 Ala Leu Gly Gly Thr Val Ala Ile Lys Val Thr Glu Phe Ser Trp Asn 6645 6650 6655 Lys Lys Leu Tyr Glu Leu Ile Gln Arg Phe Glu Tyr Trp Thr Met Phe 6660 6665 6670 Cys Thr Ser Val Asn Thr Ser Ser Ser Glu Ala Phe Leu Ile Gly Val 6675 6680 6685 His Tyr Leu Gly Asp Phe Ala Ser Gly Ala Val Ile Asp Gly Asn Thr 6690 6695 6700 Met His Ala Asn Tyr Ile Phe Trp Arg Asn Ser Thr Ile Met Thr Met 6705 6710 6715 6720 Ser Tyr Asn Ser Val Leu Asp Leu Ser Lys Phe Asn Cys Lys His Lys 6725 6730 6735 Ala Thr Val Val Ile Asn Leu Lys Asp Ser Ser Ile Ser Asp Val Val 6740 6745 6750 Leu Gly Leu Leu Lys Asn Gly Lys Leu Leu Val Arg Asn Asn Asp Ala 6755 6760 6765 Ile Cys Gly Phe Ser Asn His Leu Val Asn Val Asn Lys 6770 6775 6780 <210> 3 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces ORF3 <400> 3 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Asp Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 4 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces envelope protein <400> 4 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Pro Ser Thr Val Ile Asp Val 65 70 75 <210> 5 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces membrane protein <400> 5 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 6 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces nucleocapsid protein <400> 6 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 7 <211> 28038 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-feces complete genome <400> 7 Ala Cys Thr Thr Ala Ala Ala Gly Ala Gly Ala Thr Thr Thr Thr Cys 1 5 10 15 Thr Ala Thr Cys Thr Ala Cys Gly Gly Ala Thr Ala Gly Thr Thr Ala 20 25 30 Gly Cys Thr Cys Thr Thr Thr Thr Thr Cys Thr Ala Gly Ala Cys Thr 35 40 45 Cys Thr Thr Gly Thr Cys Thr Ala Cys Thr Cys Ala Ala Thr Thr Cys 50 55 60 Ala Ala Cys Thr Ala Ala Ala Cys Gly Ala Ala Ala Thr Thr Thr Thr 65 70 75 80 Gly Thr Cys Cys Thr Thr Cys Cys Gly Gly Cys Cys Gly Cys Ala Thr 85 90 95 Gly Thr Cys Cys Ala Thr Gly Cys Thr Gly Cys Thr Gly Gly Ala Ala 100 105 110 Gly Cys Thr Gly Ala Cys Gly Thr Gly Gly Ala Ala Thr Thr Thr Cys 115 120 125 Ala Thr Thr Ala Gly Gly Thr Thr Thr Gly Cys Thr Thr Ala Ala Gly 130 135 140 Thr Ala Gly Cys Cys Ala Thr Cys Gly Cys Ala Ala Gly Thr Gly Cys 145 150 155 160 Thr Gly Thr Gly Cys Thr Gly Thr Cys Cys Thr Cys Thr Ala Gly Thr 165 170 175 Thr Cys Cys Thr Gly Gly Thr Thr Gly Gly Cys Gly Thr Thr Cys Cys 180 185 190 Gly Thr Cys Gly Cys Cys Thr Thr Cys Thr Ala Cys Ala Thr Ala Cys 195 200 205 Thr Ala Gly Ala Cys Ala Ala Ala Cys Ala Gly Cys Cys Thr Thr Cys 210 215 220 Cys Thr Cys Cys Gly Gly Thr Thr Cys Cys Gly Thr Cys Thr Gly Gly 225 230 235 240 Gly Gly Gly Thr Thr Gly Thr Gly Thr Gly Gly Ala Thr Ala Ala Cys 245 250 255 Thr Ala Gly Thr Thr Cys Cys Gly Thr Cys Thr Ala Gly Thr Thr Thr 260 265 270 Gly Ala Ala Ala Cys Cys Ala Gly Thr Ala Ala Cys Thr Gly Thr Cys 275 280 285 Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Ala Gly Cys Ala Ala Cys 290 295 300 Cys Ala Thr Gly Thr Thr Ala Cys Ala Thr Thr Gly Gly Cys Thr Thr 305 310 315 320 Thr Thr Gly Cys Cys Ala Ala Thr Gly Ala Thr Gly Cys Ala Gly Ala 325 330 335 Ala Ala Thr Thr Thr Cys Ala Gly Cys Thr Thr Thr Thr Gly Gly Cys 340 345 350 Thr Thr Thr Thr Gly Cys Ala Cys Thr Gly Cys Thr Ala Gly Thr Gly 355 360 365 Ala Ala Gly Cys Cys Gly Thr Cys Thr Cys Ala Thr Ala Cys Thr Ala 370 375 380 Thr Thr Cys Thr Gly Ala Gly Gly Cys Cys Gly Cys Cys Gly Cys Thr 385 390 395 400 Ala Gly Thr Gly Gly Ala Thr Thr Thr Ala Thr Gly Cys Ala Ala Thr 405 410 415 Gly Cys Cys Gly Thr Thr Thr Cys Gly Thr Gly Thr Cys Cys Thr Thr 420 425 430 Cys Gly Ala Thr Cys Thr Cys Gly Cys Thr Gly Ala Cys Ala Cys Thr 435 440 445 Gly Thr Thr Gly Ala Gly Gly Gly Ala Thr Thr Gly Cys Thr Thr Cys 450 455 460 Cys Cys Gly Ala Ala Gly Ala Cys Thr Ala Thr Gly Thr Cys Ala Thr 465 470 475 480 Gly Gly Thr Gly Gly Thr Gly Gly Thr Cys Gly Gly Cys Ala Cys Thr 485 490 495 Ala Cys Cys Ala Ala Gly Cys Thr Thr Ala Gly Thr Gly Cys Gly Thr 500 505 510 Ala Thr Gly Thr Gly Gly Ala Cys Ala Cys Thr Thr Thr Thr Gly Gly 515 520 525 Thr Ala Gly Cys Cys Gly Cys Cys Cys Cys Ala Ala Ala Ala Ala Cys 530 535 540 Ala Thr Thr Thr Gly Thr Gly Gly Thr Thr Gly Gly Cys Thr Gly Thr 545 550 555 560 Thr Ala Thr Thr Thr Thr Cys Thr Ala Ala Cys Thr Gly Thr Ala Ala 565 570 575 Thr Thr Ala Cys Thr Thr Cys Cys Thr Cys Gly Ala Ala Gly Ala Gly 580 585 590 Thr Thr Ala Gly Ala Gly Cys Thr Thr Ala Cys Thr Thr Thr Thr Gly 595 600 605 Gly Thr Cys Gly Thr Cys Gly Thr Gly Gly Thr Gly Gly Thr Ala Ala 610 615 620 Cys Ala Thr Cys Gly Thr Gly Cys Cys Ala Gly Thr Thr Gly Ala Cys 625 630 635 640 Cys Ala Ala Thr Ala Cys Ala Thr Gly Thr Gly Thr Gly Gly Cys Gly 645 650 655 Cys Thr Gly Ala Cys Gly Gly Thr Ala Ala Ala Cys Cys Thr Gly Thr 660 665 670 Thr Cys Thr Thr Cys Ala Gly Gly Ala Ala Thr Cys Cys Gly Ala Ala 675 680 685 Thr Gly Gly Gly Ala Gly Thr Ala Thr Ala Cys Ala Gly Ala Thr Thr 690 695 700 Thr Cys Thr Thr Thr Gly Cys Thr Gly Ala Cys Thr Cys Cys Gly Ala 705 710 715 720 Ala Gly Ala Cys Gly Gly Thr Cys Ala Ala Cys Thr Cys Ala Ala Cys 725 730 735 Ala Thr Thr Gly Cys Thr Gly Gly Thr Ala Thr Cys Ala Cys Thr Thr 740 745 750 Ala Thr Gly Thr Gly Ala Ala Gly Gly Cys Cys Thr Gly Gly Ala Thr 755 760 765 Thr Gly Thr Ala Gly Ala Gly Cys Gly Ala Thr Cys Gly Gly Ala Thr 770 775 780 Gly Thr Cys Thr Cys Thr Thr Ala Thr Gly Cys Gly Ala Gly Thr Cys 785 790 795 800 Ala Gly Ala Ala Thr Thr Thr Ala Ala Cys Ala Thr Cys Thr Ala Thr 805 810 815 Thr Ala Ala Gly Thr Cys Thr Ala Thr Thr Ala Cys Thr Thr Ala Cys 820 825 830 Thr Gly Thr Thr Cys Ala Ala Cys Cys Thr Ala Thr Gly Ala Gly Cys 835 840 845 Ala Thr Ala Cys Thr Thr Thr Thr Cys Cys Thr Gly Ala Thr Gly Gly 850 855 860 Thr Ala Cys Thr Gly Cys Cys Ala Thr Gly Ala Ala Gly Gly Thr Thr 865 870 875 880 Gly Cys Ala Cys Gly Thr Ala Cys Thr Cys Cys Ala Ala Ala Gly Ala 885 890 895 Thr Thr Ala Ala Gly Ala Ala Gly Ala Cys Thr Gly Thr Thr Gly Thr 900 905 910 Cys Thr Thr Gly Thr Cys Thr Gly Ala Gly Cys Cys Ala Cys Thr Thr 915 920 925 Gly Cys Thr Ala Cys Thr Ala Thr Cys Thr Ala Cys Ala Gly Gly Gly 930 935 940 Ala Ala Ala Thr Thr Gly Gly Thr Thr Cys Thr Cys Cys Thr Thr Thr 945 950 955 960 Thr Gly Thr Gly Gly Ala Thr Ala Ala Thr Gly Gly Gly Ala Gly Cys 965 970 975 Gly Ala Thr Gly Cys Thr Cys Gly Thr Thr Cys Thr Ala Thr Cys Ala 980 985 990 Thr Thr Ala Ala Gly Ala Gly Ala Cys Cys Ala Gly Thr Gly Thr Thr 995 1000 1005 Cys Cys Thr Cys Cys Ala Cys Gly Cys Thr Thr Thr Thr Gly Thr Thr 1010 1015 1020 Ala Ala Gly Thr Gly Thr Ala Ala Gly Thr Gly Thr Gly Gly Thr Ala 1025 1030 1035 1040 Gly Thr Thr Ala Thr Cys Ala Thr Thr Gly Gly Ala Cys Thr Gly Thr 1045 1050 1055 Thr Gly Gly Thr Gly Ala Thr Thr Gly Gly Ala Cys Thr Thr Cys Cys 1060 1065 1070 Thr Ala Thr Gly Thr Cys Thr Cys Cys Ala Cys Thr Thr Gly Cys Thr 1075 1080 1085 Gly Thr Gly Gly Cys Thr Thr Thr Ala Ala Gly Thr Gly Thr Ala Ala 1090 1095 1100 Gly Cys Cys Ala Gly Thr Cys Cys Thr Thr Gly Thr Gly Gly Cys Thr 1105 1110 1115 1120 Thr Cys Ala Thr Gly Cys Thr Cys Thr Gly Cys Thr Ala Cys Gly Cys 1125 1130 1135 Cys Thr Gly Gly Thr Thr Cys Thr Gly Thr Thr Gly Thr Gly Gly Thr 1140 1145 1150 Thr Ala Cys Gly Cys Gly Cys Gly Cys Thr Gly Gly Thr Gly Cys Thr 1155 1160 1165 Gly Gly Cys Ala Cys Thr Gly Gly Thr Gly Thr Thr Ala Ala Gly Thr 1170 1175 1180 Ala Thr Thr Ala Cys Ala Ala Cys Ala Ala Cys Ala Thr Gly Thr Thr 1185 1190 1195 1200 Cys Cys Thr Gly Cys Gly Cys Cys Ala Thr Gly Thr Gly Gly Cys Ala 1205 1210 1215 Gly Ala Cys Ala Thr Thr Gly Ala Thr Gly Gly Gly Thr Thr Gly Gly 1220 1225 1230 Cys Ala Thr Thr Cys Thr Gly Gly Cys Gly Ala Ala Thr Thr Cys Thr 1235 1240 1245 Cys Ala Ala Gly Gly Thr Gly Cys Ala Gly Thr Cys Cys Ala Ala Ala 1250 1255 1260 Gly Ala Cys Gly Ala Cys Cys Thr Cys Gly Cys Thr Thr Gly Cys Thr 1265 1270 1275 1280 Cys Thr Gly Gly Thr Ala Ala Ala Thr Thr Cys Cys Thr Thr Gly Ala 1285 1290 1295 Ala Cys Ala Cys Cys Ala Thr Gly Ala Gly Gly Ala Ala Gly Gly Thr 1300 1305 1310 Thr Thr Cys Ala Cys Ala Gly Ala Thr Cys Cys Thr Thr Gly Cys Thr 1315 1320 1325 Ala Cys Thr Thr Thr Thr Thr Gly Ala Ala Thr Gly Ala Cys Thr Cys 1330 1335 1340 Gly Ala Gly Cys Ala Thr Thr Gly Cys Thr Ala Cys Thr Ala Ala Gly 1345 1350 1355 1360 Cys Thr Cys Ala Ala Gly Thr Thr Thr Gly Ala Cys Ala Thr Cys Cys 1365 1370 1375 Thr Thr Ala Gly Thr Gly Gly Cys Ala Ala Gly Thr Thr Thr Thr Cys 1380 1385 1390 Thr Gly Ala Thr Gly Ala Ala Gly Thr Cys Ala Ala Ala Cys Ala Ala 1395 1400 1405 Gly Cys Thr Ala Thr Cys Thr Thr Thr Gly Cys Thr Gly Gly Thr Cys 1410 1415 1420 Ala Thr Gly Thr Thr Gly Thr Thr Gly Thr Thr Gly Gly Cys Ala Gly 1425 1430 1435 1440 Cys Gly Cys Gly Cys Thr Cys Gly Thr Thr Gly Ala Cys Ala Thr Thr 1445 1450 1455 Gly Thr Thr Gly Ala Cys Gly Ala Thr Gly Cys Ala Cys Thr Gly Gly 1460 1465 1470 Gly Ala Cys Ala Gly Cys Cys Thr Thr Gly Gly Thr Thr Thr Ala Thr 1475 1480 1485 Ala Cys Gly Thr Ala Ala Gly Cys Thr Thr Gly Gly Thr Gly Ala Cys 1490 1495 1500 Cys Thr Thr Gly Cys Ala Ala Gly Thr Gly Cys Ala Gly Cys Thr Thr 1505 1510 1515 1520 Gly Gly Gly Ala Gly Cys Ala Gly Cys Thr Thr Ala Ala Gly Gly Cys 1525 1530 1535 Thr Gly Thr Cys Gly Thr Thr Ala Gly Ala Gly Gly Cys Cys Thr Thr 1540 1545 1550 Ala Ala Cys Cys Thr Cys Cys Thr Gly Thr Cys Thr Gly Ala Thr Gly 1555 1560 1565 Ala Gly Gly Thr Cys Gly Thr Gly Cys Thr Cys Thr Thr Thr Gly Gly 1570 1575 1580 Cys Ala Ala Ala Ala Gly Ala Cys Thr Thr Ala Gly Cys Thr Gly Thr 1585 1590 1595 1600 Gly Cys Cys Ala Cys Thr Cys Thr Thr Ala Gly Thr Ala Thr Cys Gly 1605 1610 1615 Thr Thr Ala Ala Cys Gly Gly Thr Gly Thr Thr Thr Thr Thr Gly Ala 1620 1625 1630 Gly Thr Thr Cys Ala Thr Cys Gly Cys Cys Gly Ala Ala Gly Thr Gly 1635 1640 1645 Cys Cys Thr Gly Ala Gly Ala Ala Gly Thr Thr Gly Gly Cys Thr Gly 1650 1655 1660 Cys Gly Gly Cys Thr Gly Thr Thr Ala Cys Ala Gly Thr Thr Thr Thr 1665 1670 1675 1680 Thr Gly Thr Cys Ala Ala Cys Thr Thr Cys Thr Thr Gly Ala Ala Thr 1685 1690 1695 Gly Ala Gly Cys Thr Thr Thr Thr Thr Gly Ala Gly Thr Cys Thr Gly 1700 1705 1710 Cys Cys Thr Gly Thr Gly Ala Cys Thr Gly Cys Thr Thr Ala Ala Ala 1715 1720 1725 Gly Gly Thr Cys Gly Gly Ala Gly Gly Thr Ala Ala Ala Ala Cys Cys 1730 1735 1740 Thr Thr Thr Ala Ala Cys Ala Ala Gly Gly Thr Thr Gly Gly Cys Thr 1745 1750 1755 1760 Cys Thr Thr Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Gly Ala 1765 1770 1775 Cys Ala Ala Cys Gly Cys Ala Thr Thr Gly Gly Thr Thr Ala Ala Gly 1780 1785 1790 Cys Thr Thr Gly Thr Cys Ala Ala Gly Gly Cys Ala Ala Ala Ala Gly 1795 1800 1805 Thr Thr Cys Gly Cys Gly Gly Cys Cys Cys Ala Cys Gly Ala Cys Ala 1810 1815 1820 Gly Gly Cys Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Gly Ala Ala 1825 1830 1835 1840 Gly Thr Thr Cys Gly Thr Thr Ala Cys Ala Cys Ala Ala Gly Cys Cys 1845 1850 1855 Thr Thr Gly Thr Thr Ala Thr Thr Gly Gly Gly Ala Gly Thr Ala Cys 1860 1865 1870 Thr Ala Cys Cys Ala Ala Gly Gly Thr Gly Gly Thr Thr Thr Cys Cys 1875 1880 1885 Ala Ala Gly Cys Gly Cys Gly Thr Thr Gly Ala Ala Ala Ala Thr Gly 1890 1895 1900 Cys Cys Ala Ala Thr Gly Thr Gly Ala Ala Thr Cys Thr Cys Gly Thr 1905 1910 1915 1920 Cys Gly Thr Cys Gly Thr Thr Gly Ala Cys Gly Ala Gly Gly Ala Thr 1925 1930 1935 Gly Thr Gly Ala Cys Cys Cys Thr Cys Ala Ala Cys Ala Cys Cys Ala 1940 1945 1950 Cys Thr Gly Gly Thr Cys Gly Thr Ala Cys Ala Gly Thr Thr Gly Thr 1955 1960 1965 Thr Gly Thr Thr Gly Ala Cys Gly Gly Ala Cys Thr Thr Gly Cys Ala 1970 1975 1980 Thr Thr Cys Thr Thr Cys Gly Ala Gly Ala Gly Thr Gly Ala Cys Gly 1985 1990 1995 2000 Gly Gly Thr Thr Thr Thr Ala Cys Ala Gly Ala Cys Ala Thr Cys Thr 2005 2010 2015 Thr Gly Cys Thr Gly Ala Thr Gly Cys Thr Gly Ala Cys Gly Thr Thr 2020 2025 2030 Gly Thr Cys Ala Thr Thr Gly Ala Ala Cys Ala Thr Cys Cys Thr Gly 2035 2040 2045 Thr Thr Thr Ala Thr Ala Ala Gly Thr Cys Thr Gly Cys Thr Thr Gly 2050 2055 2060 Thr Gly Ala Gly Cys Thr Cys Ala Ala Gly Cys Cys Ala Gly Thr Thr 2065 2070 2075 2080 Thr Thr Thr Gly Ala Gly Thr Gly Thr Gly Ala Cys Cys Cys Ala Ala 2085 2090 2095 Thr Ala Cys Cys Thr Gly Ala Thr Thr Thr Thr Cys Cys Thr Ala Thr 2100 2105 2110 Gly Cys Cys Thr Gly Thr Gly Gly Cys Cys Gly Cys Thr Ala Gly Thr 2115 2120 2125 Gly Thr Thr Gly Cys Ala Gly Ala Gly Cys Thr Thr Thr Gly Thr Gly 2130 2135 2140 Thr Gly Cys Ala Ala Ala Cys Thr Gly Ala Thr Cys Thr Gly Thr Thr 2145 2150 2155 2160 Gly Cys Thr Thr Ala Ala Ala Ala Ala Thr Thr Ala Cys Ala Ala Cys 2165 2170 2175 Ala Cys Thr Cys Cys Thr Thr Ala Thr Ala Ala Ala Ala Cys Thr Thr 2180 2185 2190 Ala Cys Ala Gly Cys Thr Gly Cys Gly Thr Thr Gly Thr Gly Ala Gly 2195 2200 2205 Ala Gly Gly Thr Gly Ala Thr Ala Ala Gly Thr Gly Thr Thr Gly Cys 2210 2215 2220 Ala Thr Cys Ala Cys Thr Thr Gly Cys Ala Cys Cys Thr Thr Ala Cys 2225 2230 2235 2240 Ala Thr Ala Thr Cys Ala Cys Ala Gly Cys Ala Cys Cys Ala Ala Gly 2245 2250 2255 Thr Thr Ala Thr Ala Thr Gly Gly Ala Gly Gly Ala Thr Gly Cys Thr 2260 2265 2270 Gly Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Ala Gly Ala Cys Cys 2275 2280 2285 Thr Cys Thr Gly Thr Ala Cys Cys Ala Ala Gly Ala Ala Cys Ala Thr 2290 2295 2300 Thr Gly Gly Thr Ala Cys Thr Gly Cys Thr Gly Gly Thr Thr Thr Thr 2305 2310 2315 2320 Cys Ala Thr Gly Ala Gly Thr Thr Thr Thr Ala Cys Ala Thr Thr Ala 2325 2330 2335 Cys Gly Gly Cys Cys Cys Ala Thr Gly Ala Ala Cys Ala Ala Cys Ala 2340 2345 2350 Gly Gly Ala Thr Cys Thr Gly Cys Ala Ala Gly Gly Gly Thr Thr Cys 2355 2360 2365 Gly Thr Ala Ala Cys Cys Ala Cys Thr Thr Gly Thr Thr Gly Cys Ala 2370 2375 2380 Cys Gly Ala Thr Gly Thr Cys Ala Gly Gly Thr Thr Thr Thr Gly Ala 2385 2390 2395 2400 Gly Thr Gly Thr Thr Thr Thr Ala Thr Gly Cys Cys Thr Ala Thr Ala 2405 2410 2415 Ala Thr Cys Cys Cys Ala Cys Ala Gly Thr Gly Thr Cys Cys Ala Gly 2420 2425 2430 Cys Ala Gly Thr Gly Cys Thr Thr Gly Ala Ala Gly Ala Gly Ala Thr 2435 2440 2445 Thr Gly Ala Thr Gly Gly Thr 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2645 2650 2655 Ala Ala Gly Thr Ala Thr Thr Ala Thr Gly Cys Cys Ala Cys Cys Ala 2660 2665 2670 Gly Thr Gly Thr Cys Cys Cys Ala Ala Ala Ala Ala Thr Thr Gly Thr 2675 2680 2685 Thr Thr Thr Gly Gly Gly Cys Thr Gly Thr Thr Gly Thr Thr Thr Thr 2690 2695 2700 Cys Ala Cys Ala Gly Thr Gly Thr Thr Ala Ala Ala Ala Gly Thr Gly 2705 2710 2715 2720 Thr Thr Cys Thr Thr Gly Cys Ala Ala Gly Thr Gly Cys Cys Thr Thr 2725 2730 2735 Cys Cys Ala Gly Ala Thr Thr Cys Cys Thr Gly Thr Cys Cys Ala Gly 2740 2745 2750 Gly Cys Ala Gly Gly Cys Ala Thr Thr Gly Ala Gly Ala Ala Gly Thr 2755 2760 2765 Thr Thr Ala Ala Ala Gly Thr Cys Thr Thr Cys Cys Thr Thr Ala Ala 2770 2775 2780 Cys Thr Gly Thr Gly Thr Thr Cys Ala Cys Cys Cys Thr Gly Thr Thr 2785 2790 2795 2800 Gly Thr Ala Cys Cys Ala Cys Gly Cys Gly Thr Cys Ala Thr Thr Gly 2805 2810 2815 Ala Ala Ala Cys Thr Thr Cys Thr Thr Thr Thr Gly Thr Gly Gly Ala 2820 2825 2830 Ala Thr Thr Ala Gly Ala Ala Gly Ala Gly Ala Cys Gly Ala Cys Ala 2835 2840 2845 Thr Thr Thr Ala Ala Ala Cys 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3045 3050 3055 Ala Gly Thr Cys Thr Gly Ala Gly Ala Cys Thr Ala Thr Thr Ala Thr 3060 3065 3070 Gly Gly Cys Thr Gly Thr Gly Cys Thr Thr Ala Ala Thr Ala Ala Gly 3075 3080 3085 Gly Cys Thr Gly Thr Thr Gly Gly Thr Ala Ala Thr Cys Gly Thr Ala 3090 3095 3100 Thr Cys Ala Ala Gly Gly Thr Thr Ala Cys Ala Gly Gly Thr Gly Gly 3105 3110 3115 3120 Thr Thr Gly Gly Gly Ala Cys Gly Ala Thr Gly Thr Thr Gly Thr Thr 3125 3130 3135 Gly Ala Gly Thr Ala Thr Ala Thr Cys Ala Ala Cys Gly Thr Thr Gly 3140 3145 3150 Cys Cys Ala Thr Thr Gly Ala Gly Gly Thr Thr Cys Thr Thr Ala Ala 3155 3160 3165 Ala Gly Ala Thr Cys Ala Thr Ala Thr Cys Gly Ala Thr Gly Thr Gly 3170 3175 3180 Cys Cys Thr Ala Ala Gly Thr Ala Cys Thr Ala Cys Ala Thr Cys Thr 3185 3190 3195 3200 Ala Thr Gly Ala Thr Gly Ala Gly Gly Ala Ala Gly Gly Thr Gly Gly 3205 3210 3215 Cys Ala Cys Cys Gly Ala Thr Cys Cys Thr Ala Ala Thr Cys Thr Thr 3220 3225 3230 Cys Cys Cys Gly Thr Ala Ala Thr Gly Gly Thr Thr Thr Cys Thr Cys 3235 3240 3245 Ala Gly Thr Gly Gly Cys Cys Gly Thr Thr Gly Ala Ala Thr Gly Ala 3250 3255 3260 Thr Gly Ala Cys Ala Cys Gly Ala Thr Cys Thr Cys Ala Cys Ala Gly 3265 3270 3275 3280 Gly Ala Thr Cys Thr Gly Cys Thr Thr Gly Ala Thr Gly Thr Gly Gly 3285 3290 3295 Ala Ala Gly Thr Thr Gly Thr Thr Ala Cys Thr Gly Ala Thr Gly Cys 3300 3305 3310 Ala Cys Cys Ala Ala Thr Thr Gly Ala Thr Thr Thr Cys Gly Ala Gly 3315 3320 3325 Gly Gly Thr Gly Ala Thr Gly Ala Ala Gly Thr Ala Gly Ala Cys Thr 3330 3335 3340 Cys Cys Thr Cys Thr Gly Ala Cys Cys Cys Thr Gly Ala Thr Ala Ala 3345 3350 3355 3360 Gly Gly Thr Gly Gly Cys Ala Gly Ala Thr Gly Thr Gly Gly Cys Thr 3365 3370 3375 Ala Ala Cys Thr Cys Thr Gly Ala Gly Cys Cys Thr Gly Ala Gly Gly 3380 3385 3390 Ala Thr Gly Ala Thr Gly Gly Thr Cys Cys Thr Ala Ala Thr Gly Thr 3395 3400 3405 Ala Gly Cys Thr Cys Cys Thr Gly Ala Ala Ala Cys Ala Ala Ala Thr 3410 3415 3420 Gly Thr Ala Gly Ala Gly Thr Cys Thr Gly Ala Ala Gly Thr Thr Gly 3425 3430 3435 3440 Ala Gly Gly Ala Ala Gly Thr Thr Gly Cys Cys Gly Cys Ala Ala Cys 3445 3450 3455 Cys Thr Thr Gly Thr Cys Thr Thr Thr Thr Ala Thr Thr Ala Ala Ala 3460 3465 3470 Gly Ala Thr Ala Cys Ala Cys Cys Thr Thr Cys Cys Ala Cys Ala Gly 3475 3480 3485 Thr Thr Ala Cys Thr Ala Ala Gly Gly Ala Thr Cys Cys Thr Thr Thr 3490 3495 3500 Thr Gly Cys Thr Thr Thr Thr Gly Ala Cys Thr Thr Thr Gly Cys Ala 3505 3510 3515 3520 Ala Gly Cys Thr Ala Thr Gly Gly Ala Gly Gly Ala Cys Thr Thr Ala 3525 3530 3535 Ala Gly Gly Thr Thr Thr Thr Ala Ala Gly Ala Cys Ala Ala Thr Cys 3540 3545 3550 Thr Cys Ala Thr Ala Ala Cys Ala Ala Cys Thr Gly Cys Thr Gly Gly 3555 3560 3565 Gly Thr Thr Ala Cys Thr Thr Cys Thr Ala Cys Cys Thr Thr Gly Gly 3570 3575 3580 Thr Gly Cys Ala Gly Cys Thr Ala Cys Ala Ala Thr Thr Gly Cys Thr 3585 3590 3595 3600 Thr Gly Gly Cys Ala Thr Cys Gly Thr Thr Gly Ala Thr Gly Ala Cys 3605 3610 3615 Cys Cys Thr Gly Cys Ala Ala Thr Gly Gly Ala Gly Cys Thr Thr Thr 3620 3625 3630 Thr Thr Ala Gly Thr Gly Cys Thr Gly Gly Thr Ala Gly Ala Gly Thr 3635 3640 3645 Thr Gly Gly Thr Cys Cys Ala Ala Thr Gly Gly Thr Thr Cys Gly Cys 3650 3655 3660 Ala Ala Ala Thr Gly Cys Thr Ala Thr Gly Ala Gly Thr Cys Ala Cys 3665 3670 3675 3680 Ala Ala Ala Ala Gly Gly Cys Thr Ala Thr Cys Thr Thr Gly Gly Gly 3685 3690 3695 Ala Thr Cys Thr Thr Thr Gly Gly Gly Thr Gly Ala Thr Gly Thr Gly 3700 3705 3710 Thr Cys Gly Gly Cys Thr Thr Gly Cys Cys Thr Ala Gly Ala Gly Thr 3715 3720 3725 Cys Thr Cys Thr Gly Ala Cys Thr Ala Ala Gly Gly Ala Cys Cys Thr 3730 3735 3740 Ala Cys Ala Cys Ala Cys Ala Cys Thr Thr Ala Ala Gly Ala Thr Thr 3745 3750 3755 3760 Ala Cys Cys Thr Gly Thr Thr Cys Thr Gly Thr Ala Gly Thr Cys Thr 3765 3770 3775 Gly Thr Gly Gly Thr Thr Gly Thr Gly Gly Thr Ala Cys Thr Gly Gly 3780 3785 3790 Thr Gly Ala Ala Cys Gly Thr Ala Thr Cys Thr Ala Thr Gly Ala Gly 3795 3800 3805 Gly Gly Thr Thr Gly Thr Gly Cys Thr Thr Thr Thr Cys Gly Thr Ala 3810 3815 3820 Thr Gly Ala Cys Gly Cys Cys Ala Ala Cys Thr Thr Thr Gly Gly Ala 3825 3830 3835 3840 Ala Cys Cys Gly Thr Thr Cys Cys Cys Ala Thr Ala Thr Gly Gly Thr 3845 3850 3855 Gly Cys Thr Thr Gly Thr Gly Cys Thr Cys Ala Gly Thr Gly Thr Gly 3860 3865 3870 Cys Thr Cys Ala Ala Gly Thr Thr Thr Thr Gly Ala Thr Gly Cys Ala 3875 3880 3885 Cys Ala Cys Thr Thr Thr Thr Ala Ala Ala Ala Gly Thr Ala Thr Thr 3890 3895 3900 Gly Thr Thr Gly Gly Cys Ala Cys Cys Gly Gly Cys Ala Thr Cys Thr 3905 3910 3915 3920 Thr Thr Thr Gly Thr Cys Gly Ala Gly Ala Thr Ala Cys Thr Ala Cys 3925 3930 3935 Thr Gly Cys Thr Cys Thr Cys Thr Cys Cys Thr Thr Gly Gly Ala Thr 3940 3945 3950 Thr Cys Thr Thr Thr Gly Gly Thr Thr Gly Thr Ala Ala Ala Ala Cys 3955 3960 3965 Cys Thr Cys Thr Thr Thr Gly Thr Gly Cys Gly Gly Cys Thr Gly Cys 3970 3975 3980 Thr Thr Thr Thr Ala Thr Ala Gly Gly Cys Ala Ala Gly Gly Ala Cys 3985 3990 3995 4000 Ala Gly Thr Gly Gly Thr Cys Ala Thr Thr Ala Thr Gly Thr Cys Ala 4005 4010 4015 Cys Thr Ala Ala Cys Thr Thr Thr Thr Ala Thr Gly Ala Thr Gly Cys 4020 4025 4030 Thr Gly Cys Thr Ala Thr Gly Gly Cys Thr Ala Thr Thr Gly Ala Thr 4035 4040 4045 Gly Gly Thr Thr Ala Thr Gly 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25985 25990 25995 26000 Gly Ala Cys Ala Cys Ala Thr Thr Cys Thr Thr Gly Gly Thr Gly Gly 26005 26010 26015 Thr Cys Thr Thr Thr Cys Ala Ala Thr Cys Cys Thr Gly Ala Ala Ala 26020 26025 26030 Cys Ala Gly Ala Cys Gly Cys Gly Cys Thr Thr Cys Thr Cys Ala Cys 26035 26040 26045 Thr Ala Cys Thr Thr Cys Thr Gly Thr Gly Ala Thr Gly Gly Gly Cys 26050 26055 26060 Cys Gly Ala Cys Ala Gly Gly Thr Cys Thr Gly Cys Ala Thr Thr Cys 26065 26070 26075 26080 Cys Ala Gly Thr Gly Cys Thr Thr Gly Gly Ala Gly Cys Ala Cys Cys 26085 26090 26095 Ala Ala Cys Thr Gly Gly Thr Gly Thr Ala Ala Cys Gly Cys Thr Ala 26100 26105 26110 Ala Cys Ala Cys Thr Cys Cys Thr Thr Ala Gly Thr Gly Gly Thr Ala 26115 26120 26125 Cys Ala Thr Thr Gly Cys Thr Thr Gly Thr Ala Gly Ala Gly Gly Gly 26130 26135 26140 Cys Thr Ala Thr Ala Ala Gly Gly Thr Thr Gly Cys Thr Ala Cys Thr 26145 26150 26155 26160 Gly Gly Cys Gly Thr Ala Cys Ala Gly Gly Thr Ala Ala Gly Thr Cys 26165 26170 26175 Ala Ala Thr Thr Ala Cys Cys Thr Ala Ala Thr Thr Thr Cys Gly Thr 26180 26185 26190 Cys Ala Cys Ala Gly Thr Cys Gly Cys Cys Ala Ala Gly Gly Cys Cys 26195 26200 26205 Ala Cys Thr Ala Cys Ala Ala Cys Ala Ala Thr Thr Gly Thr Cys Thr 26210 26215 26220 Ala Cys Gly Gly Ala Cys Gly Thr Gly Thr Thr Gly Gly Thr Cys Gly 26225 26230 26235 26240 Thr Thr Cys Ala Gly Thr Cys Ala Ala Thr Gly Cys Thr Thr Cys Ala 26245 26250 26255 Thr Cys Thr Gly Gly Cys Ala Cys Thr Gly Gly Thr Thr Gly Gly Gly 26260 26265 26270 Cys Thr Thr Thr Cys Thr Ala Thr Gly Thr Cys Cys Gly Gly Thr Cys 26275 26280 26285 Cys Ala Ala Ala Cys Ala Cys Gly Gly Cys Gly Ala Cys Thr Ala Cys 26290 26295 26300 Thr Cys Ala Gly Cys Thr Gly Thr Gly Ala Gly Thr Ala Ala Thr Cys 26305 26310 26315 26320 Cys Gly Ala Gly Thr Thr Cys Gly Gly Thr Thr Cys Thr Cys Ala Cys 26325 26330 26335 Ala Gly Ala Thr Ala Gly Thr Gly Ala Gly Ala Ala Ala Gly Thr Gly 26340 26345 26350 Cys Thr Thr Cys Ala Thr Thr Thr Ala Gly Thr Cys Thr Ala Ala Ala 26355 26360 26365 Cys Ala Gly Ala Ala Ala Cys Thr Thr Thr Ala Thr Gly Gly Cys Thr 26370 26375 26380 Thr Cys Thr Gly Thr Cys Ala Gly Thr Thr Thr Thr Cys Ala Gly Gly 26385 26390 26395 26400 Ala Thr Cys Gly Thr Gly Gly Cys Cys Gly Cys Ala Ala Ala Cys Gly 26405 26410 26415 Gly Gly Thr Gly Cys Cys Ala Thr Thr Ala Thr Cys Cys Cys Thr Cys 26420 26425 26430 Thr Ala Thr Gly Cys Cys Cys Cys Thr Cys Thr Thr Ala Gly Gly Gly 26435 26440 26445 Thr Thr Ala Cys Thr Ala Ala Thr Gly Ala Cys Ala Ala Ala Cys Cys 26450 26455 26460 Cys Cys Thr Thr Thr Cys Thr Ala Ala Gly Gly Thr Ala Cys Thr Thr 26465 26470 26475 26480 Gly Cys Ala Ala Ala Thr Ala Ala Thr Gly Cys Thr Gly Thr Ala Cys 26485 26490 26495 Cys Cys Ala Cys Thr Ala Ala Thr Ala Ala Ala Gly Gly Ala Ala Ala 26500 26505 26510 Thr Ala Ala Gly Gly Ala Cys Cys Ala Gly Cys Ala Ala Ala Thr Thr 26515 26520 26525 Gly Gly Ala Thr Ala Cys Thr Gly Gly Ala Ala Thr Gly Ala Gly Cys 26530 26535 26540 Ala Ala Ala Thr Thr Cys Gly Cys Thr Gly Gly Cys Gly Cys Ala Thr 26545 26550 26555 26560 Gly Cys Gly Cys Cys Gly Thr Gly Gly Thr Gly Ala Gly Cys Gly Ala 26565 26570 26575 Ala Thr Thr Gly Ala Ala Cys Ala Ala Cys Cys Thr Thr Cys Cys Ala 26580 26585 26590 Ala Thr Thr Gly Gly Cys Ala Thr Thr Thr Cys Thr Ala Cys Thr Ala 26595 26600 26605 Cys Cys Thr Cys Gly Gly Ala Ala Cys Ala Gly Gly Ala Cys Cys Thr 26610 26615 26620 Cys Ala Cys Gly Cys Cys Gly Ala Cys Cys Thr Cys Cys Gly Cys Thr 26625 26630 26635 26640 Ala Thr Ala Gly Gly Ala Cys Thr Cys Gly Thr Ala Cys Thr Gly Ala 26645 26650 26655 Gly Gly Gly Thr Gly Thr Thr Thr Thr Cys Thr Gly Gly Gly Thr Thr 26660 26665 26670 Gly Cys Thr Ala Ala Ala Gly Ala Ala Gly Gly Cys Gly Cys Ala Ala 26675 26680 26685 Ala Gly Ala Cys Thr Gly Ala Ala Cys Cys Cys Ala Cys Thr Ala Ala 26690 26695 26700 Cys Cys Thr Gly Gly Gly Thr Gly Thr Cys Ala Gly Ala Ala Ala Gly 26705 26710 26715 26720 Gly Cys Gly Thr Cys Thr Gly Ala Ala Ala Ala Gly Cys Cys Ala Ala 26725 26730 26735 Thr Thr Ala Thr Thr Cys Cys Ala Ala Ala Thr Thr Thr Cys Thr Cys 26740 26745 26750 Thr Cys Ala Ala Cys Ala Gly Cys Thr Thr Cys Cys Cys Ala Gly Cys 26755 26760 26765 Gly Thr Ala Gly Thr Thr Gly Ala Gly Ala Thr Thr Gly Thr Thr Gly 26770 26775 26780 Ala Ala Cys Cys Thr Ala Ala Cys Ala Cys Ala Cys Cys Thr Cys Cys 26785 26790 26795 26800 Thr Ala Cys Thr Thr Cys Ala Cys Gly Thr Gly Cys Ala Ala Ala Thr 26805 26810 26815 Thr Cys Ala Cys Gly Thr Ala Gly Cys Ala Gly Gly Ala Gly Thr Cys 26820 26825 26830 Gly Thr Gly Gly Thr Ala Ala Thr Gly Gly Cys Ala Ala Cys Ala Ala 26835 26840 26845 Cys Ala Gly Gly Thr Cys Cys Ala Gly Ala Thr Cys Thr Cys Cys Ala 26850 26855 26860 Ala Gly Thr Ala Ala Cys Ala Ala Cys Ala Gly Ala Gly Gly Cys Ala 26865 26870 26875 26880 Ala Thr Ala Ala Cys Cys Ala Gly Thr Cys Cys Cys Gly Cys Gly Gly 26885 26890 26895 Thr Ala Ala Thr Thr Cys Ala Cys Ala Gly Ala Ala Thr Cys Gly Thr 26900 26905 26910 Gly Gly Ala Ala Ala Thr Ala Ala Cys Cys Ala Gly Gly Gly Thr Cys 26915 26920 26925 Gly Thr Gly Gly Ala Gly Cys Thr Thr Cys Thr Cys Ala Gly Ala Ala 26930 26935 26940 Cys Ala Gly Ala Gly Gly Ala Gly Gly Cys Ala Ala Thr Ala Ala Thr 26945 26950 26955 26960 Ala Ala Thr Ala Ala Cys Ala Ala Thr Ala Ala Cys Ala Ala Gly Thr 26965 26970 26975 Cys Thr Cys Gly Thr Ala Ala Cys Cys Ala Gly Thr Cys Cys Ala Ala 26980 26985 26990 Gly Ala Ala Cys Ala Gly Ala Ala Ala Cys Cys Ala Gly Thr Cys Ala 26995 27000 27005 Ala Ala Thr Gly Ala Cys Cys Gly Thr Gly Gly Thr Gly Gly Thr Gly 27010 27015 27020 Thr Ala Ala Cys Ala Thr Cys Ala Cys Gly Cys Gly Ala Thr Gly Ala 27025 27030 27035 27040 Thr Cys Thr Gly Gly Thr Gly Gly Cys Thr Gly Cys Thr Gly Thr Cys 27045 27050 27055 Ala Ala Gly Gly Ala Thr Gly Cys Cys Cys Thr Thr Ala Ala Ala Thr 27060 27065 27070 Cys Thr Thr Thr Gly Gly Gly Thr Ala Thr Thr Gly Gly Cys Gly Ala 27075 27080 27085 Ala Ala Ala Cys Cys Cys Thr Gly Ala Cys Ala Ala Gly Cys Thr Thr 27090 27095 27100 Ala Ala Gly Cys Ala Ala Cys Ala Gly Cys Ala Gly Ala Ala Gly Cys 27105 27110 27115 27120 Cys Cys Ala Ala Ala Cys Ala Gly Gly Ala Ala Ala Gly Gly Thr Cys 27125 27130 27135 Thr Gly Ala Cys Ala Gly Cys Ala Gly Cys Gly Gly Cys Ala Ala Ala 27140 27145 27150 Ala Ala Thr Ala Cys Ala Cys Cys Thr Ala Ala Gly Ala Ala Gly Ala 27155 27160 27165 Ala Cys Ala Ala Ala Thr Cys Cys Ala Gly Ala Gly Cys Cys Ala Cys 27170 27175 27180 Thr Thr Cys Gly Ala Ala Ala Gly Ala Ala Cys Gly Thr Gly Ala Cys 27185 27190 27195 27200 Cys Thr Cys Ala Ala Ala Gly Ala Cys Ala Thr Cys Cys Cys Ala Gly 27205 27210 27215 Ala Gly Thr Gly Gly Ala Gly Gly Ala Gly Ala Ala Thr Thr Cys Cys 27220 27225 27230 Cys Ala Ala Gly Gly Gly Cys Gly Ala Ala Ala Ala Thr Ala Gly Cys 27235 27240 27245 Gly Thr Ala Gly Cys Ala Gly Cys Thr Thr Gly Cys Thr Thr Cys Gly 27250 27255 27260 Gly Ala Cys Cys Cys Ala Gly Gly Gly Gly Ala Gly Gly Cys Thr Thr 27265 27270 27275 27280 Cys Ala Ala Ala Ala Ala Thr Thr Thr Thr Gly Gly Ala Gly Ala Thr 27285 27290 27295 Gly Cys Gly Gly Ala Ala Thr Thr Thr Gly Thr Cys Gly Ala Ala Ala 27300 27305 27310 Ala Ala Gly Gly Thr Gly Thr Thr Gly Ala Thr Gly Cys Cys Thr Cys 27315 27320 27325 Ala Gly Gly Cys Thr Ala Thr Gly Cys Thr Cys Ala Gly Ala Thr Cys 27330 27335 27340 Gly Cys Cys Ala Gly Thr Thr Thr Ala Gly Cys Ala Cys Cys Ala Ala 27345 27350 27355 27360 Ala Thr Gly Thr Thr Gly Cys Ala Gly Cys Ala Thr Thr Gly Cys Thr 27365 27370 27375 Cys Thr Thr Thr Gly Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Gly 27380 27385 27390 Gly Cys Thr Gly Thr Thr Cys Gly Thr Gly Ala Gly Cys Thr Ala Gly 27395 27400 27405 Cys Gly Gly Ala Cys Thr Cys Thr Thr Ala Cys Gly Ala Gly Ala Thr 27410 27415 27420 Thr Ala Cys Ala Thr Ala Thr Ala Ala Thr Thr Ala Thr Ala Ala Ala 27425 27430 27435 27440 Ala Thr Gly Ala Cys Thr Gly Thr Gly Cys Cys Ala Ala Ala Gly Thr 27445 27450 27455 Cys Thr Gly Ala Thr Cys Cys Ala Ala Ala Thr Gly Thr Ala Gly Ala 27460 27465 27470 Gly Cys Thr Thr Cys Thr Thr Gly Thr Thr Thr Cys Ala Cys Ala Gly 27475 27480 27485 Gly Thr Gly Gly Ala Thr Gly Cys Ala Thr Thr Thr Ala Ala Ala Ala 27490 27495 27500 Cys Thr Gly Gly Gly Ala Ala Thr Gly Cys Ala Ala Ala Ala Cys Cys 27505 27510 27515 27520 Cys Cys Ala Gly Ala Gly Ala Ala Ala Gly Ala Ala Gly Gly Ala Ala 27525 27530 27535 Ala Ala Gly Ala Ala Gly Ala Ala Cys Ala Ala Gly Cys Gly Thr Gly 27540 27545 27550 Ala Ala Ala Cys Cys Ala Cys Gly Cys Ala Gly Cys Ala Gly Cys Thr 27555 27560 27565 Gly Ala Ala Thr Gly Ala Ala Gly Ala Gly Gly Cys Cys Ala Thr Cys 27570 27575 27580 Thr Ala Cys Gly Ala Thr Gly Ala Thr Gly Thr Gly Gly Gly Thr Gly 27585 27590 27595 27600 Thr Gly Cys Cys Ala Thr Cys Thr Gly Ala Thr Gly Thr Gly Ala Cys 27605 27610 27615 Thr Cys Ala Thr Gly Cys Cys Ala Ala Thr Thr Thr Gly Gly Ala Ala 27620 27625 27630 Thr Gly Gly Gly Ala Cys Ala Cys Ala Gly Cys Thr Gly Thr Thr Gly 27635 27640 27645 Ala Thr Gly Gly Thr Gly Gly Thr Gly Ala Cys Ala Cys Gly Gly Cys 27650 27655 27660 Cys Gly Thr Thr Gly Ala Ala Ala Thr Thr Ala Thr Cys Ala Ala Cys 27665 27670 27675 27680 Gly Ala Gly Ala Thr Cys Thr Thr Cys Gly Ala Cys Ala Cys Ala Gly 27685 27690 27695 Gly Ala Ala Ala Thr Thr Ala Ala Ala Cys Ala Ala Thr Gly Thr Thr 27700 27705 27710 Thr Gly Ala Cys Thr Gly Gly Cys Thr Thr Ala Thr Cys Cys Thr Gly 27715 27720 27725 Gly Cys Thr Ala Thr Gly Thr Cys Cys Cys Ala Gly Gly Gly Thr Ala 27730 27735 27740 Gly Thr Gly Cys Cys Ala Thr Thr Ala Cys Ala Cys Thr Gly Thr Thr 27745 27750 27755 27760 Ala Thr Thr Ala Cys Thr Gly Ala Gly Thr Gly Thr Thr Thr Thr Thr 27765 27770 27775 Cys Thr Ala Gly Cys Gly Ala Cys Thr Thr Gly Gly Cys Thr Gly Cys 27780 27785 27790 Thr Gly Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Thr Thr Gly Cys 27795 27800 27805 Cys Cys Thr Cys Thr Ala Ala Cys Thr Ala Gly Cys Gly Gly Thr Cys 27810 27815 27820 Thr Thr Gly Gly Thr Cys Thr Thr Gly Cys Ala Cys Ala Cys Ala Ala 27825 27830 27835 27840 Cys Gly Gly Thr Ala Ala Gly Cys Cys Ala Gly Thr Gly Gly Thr Ala 27845 27850 27855 Ala Thr Gly Thr Cys Ala Gly Thr Gly Cys Ala Ala Gly Ala Ala Gly 27860 27865 27870 Gly Ala Thr Ala Thr Thr Ala Cys Cys Ala Thr Ala Gly Cys Ala Cys 27875 27880 27885 Thr Gly Thr Cys Ala Thr Gly Ala Gly Gly Gly Gly Ala Ala Cys Gly 27890 27895 27900 Cys Ala Gly Thr Ala Cys Cys Thr Thr Thr Thr Cys Ala Thr Cys Thr 27905 27910 27915 27920 Ala Ala Ala Cys Cys Thr Thr Thr Gly Cys Ala Cys Gly Ala Gly Thr 27925 27930 27935 Ala Ala Thr Cys Ala Ala Ala Gly Ala Thr Cys Cys Gly Cys Thr Thr 27940 27945 27950 Gly Ala Cys Gly Ala Gly Cys Cys Thr Ala Thr Ala Thr Gly Gly Ala 27955 27960 27965 Ala Gly Ala Gly Cys Gly Thr Gly Cys Cys Ala Gly Gly Thr Ala Thr 27970 27975 27980 Thr Thr Gly Ala Cys Thr Cys Ala Ala Gly Gly Ala Cys Thr Gly Thr 27985 27990 27995 28000 Thr Ala Gly Thr Ala Ala Cys Thr Gly Ala Ala Gly Ala Cys Cys Thr 28005 28010 28015 Gly Ala Cys Gly Gly Thr Gly Thr Thr Gly Ala Thr Ala Thr Gly Gly 28020 28025 28030 Ala Thr Ala Cys Ala Cys 28035 <210> 8 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-p5 spike protein <400> 8 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 9 <211> 6781 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 ORF1a/1b <400> 9 Met Ala Ser Asn His Val Thr Leu Ala Phe Ala Asn Asp Ala Glu Ile 1 5 10 15 Ser Ala Phe Gly Phe Cys Thr Ala Ser Glu Ala Val Ser Tyr Tyr Ser 20 25 30 Glu Ala Ala Ala Ser Gly Phe Met Gln Cys Arg Phe Val Ser Phe Asp 35 40 45 Leu Ala Asp Thr Val Glu Gly Leu Leu Pro Glu Asp Tyr Val Met Val 50 55 60 Val Val Gly Thr Thr Lys Leu Ser Ala Tyr Val Asp Thr Phe Gly Ser 65 70 75 80 Arg Pro Lys Asn Ile Cys Gly Trp Leu Leu Phe Ser Asn Cys Asn Tyr 85 90 95 Phe Leu Glu Glu Leu Glu Leu Thr Phe Gly Arg Arg Gly Gly Asn Ile 100 105 110 Val Pro Val Asp Gln Tyr Met Cys Gly Ala Asp Gly Lys Pro Val Leu 115 120 125 Gln Glu Ser Glu Trp Glu Tyr Thr Asp Phe Phe Ala Asp Ser Glu Asp 130 135 140 Gly Gln Leu Asn Ile Ala Gly Ile Thr Tyr Val Lys Ala Trp Ile Val 145 150 155 160 Glu Arg Ser Asp Val Ser Tyr Ala Ser Gln Asn Leu Thr Ser Ile Lys 165 170 175 Ser Ile Thr Tyr Cys Ser Thr Tyr Glu His Thr Phe Pro Asp Gly Thr 180 185 190 Ala Met Lys Val Ala Arg Thr Pro Lys Ile Lys Lys Thr Val Val Leu 195 200 205 Ser Glu Pro Leu Ala Thr Ile Tyr Arg Glu Ile Gly Ser Pro Phe Val 210 215 220 Asp Asn Gly Ser Asp Ala Arg Ser Ile Ile Lys Arg Pro Val Phe Leu 225 230 235 240 His Ala Phe Val Lys Cys Lys Cys Gly Ser Tyr His Trp Thr Val Gly 245 250 255 Asp Trp Thr Ser Tyr Val Ser Thr Cys Cys Gly Phe Lys Cys Lys Pro 260 265 270 Val Leu Val Ala Ser Cys Ser Ala Thr Pro Gly Ser Val Val Val Thr 275 280 285 Arg Ala Gly Ala Gly Thr Gly Val Lys Tyr Tyr Asn Asn Met Phe Leu 290 295 300 Arg His Val Ala Asp Ile Asp Gly Leu Ala Phe Trp Arg Ile Leu Lys 305 310 315 320 Val Gln Ser Lys Asp Asp Leu Ala Cys Ser Gly Lys Phe Leu Glu His 325 330 335 His Glu Glu Gly Phe Thr Asp Pro Cys Tyr Phe Leu Asn Asp Ser Ser 340 345 350 Ile Ala Thr Lys Leu Lys Phe Asp Ile Leu Ser Gly Lys Phe Ser Asp 355 360 365 Glu Val Lys Gln Ala Ile Phe Ala Gly His Val Val Val Gly Ser Ala 370 375 380 Leu Val Asp Ile Val Asp Asp Ala Leu Gly Gln Pro Trp Phe Ile Arg 385 390 395 400 Lys Leu Gly Asp Leu Ala Ser Ala Ala Trp Glu Gln Leu Lys Ala Val 405 410 415 Val Arg Gly Leu Asn Leu Leu Ser Asp Glu Val Val Leu Phe Gly Lys 420 425 430 Arg Leu Ser Cys Ala Thr Leu Ser Ile Val Asn Gly Val Phe Glu Phe 435 440 445 Ile Ala Glu Val Pro Glu Lys Leu Ala Ala Ala Val Thr Val Phe Val 450 455 460 Asn Phe Leu Asn Glu Leu Phe Glu Ser Ala Cys Asp Cys Leu Lys Val 465 470 475 480 Gly Gly Lys Thr Phe Asn Lys Val Gly Ser Tyr Val Leu Phe Asp Asn 485 490 495 Ala Leu Val Lys Leu Val Lys Ala Lys Val Arg Gly Pro Arg Gln Ala 500 505 510 Gly Val Cys Glu Val Arg Tyr Thr Ser Leu Val Ile Gly Ser Thr Thr 515 520 525 Lys Val Val Ser Lys Arg Val Glu Asn Ala Asn Val Asn Leu Val Val 530 535 540 Val Asp Glu Asp Val Thr Leu Asn Thr Thr Gly Arg Thr Val Val Val 545 550 555 560 Asp Gly Leu Ala Phe Phe Glu Ser Asp Gly Phe Tyr Arg His Leu Ala 565 570 575 Asp Ala Asp Val Val Ile Glu His Pro Val Tyr Lys Ser Ala Cys Glu 580 585 590 Leu Lys Pro Val Phe Glu Cys Asp Pro Ile Pro Asp Phe Pro Met Pro 595 600 605 Val Ala Ala Ser Val Ala Glu Leu Cys Val Gln Thr Asp Leu Leu Leu 610 615 620 Lys Asn Tyr Asn Thr Pro Tyr Lys Thr Tyr Ser Cys Val Val Arg Gly 625 630 635 640 Asp Lys Cys Cys Ile Thr Cys Thr Leu His Ile Thr Ala Pro Ser Tyr 645 650 655 Met Glu Asp Ala Ala Asn Phe Val Asp Leu Cys Thr Lys Asn Ile Gly 660 665 670 Thr Ala Gly Phe His Glu Phe Tyr Ile Thr Ala His Glu Gln Gln Asp 675 680 685 Leu Gln Gly Phe Val Thr Thr Cys Cys Thr Met Ser Gly Phe Glu Cys 690 695 700 Phe Met Pro Ile Ile Pro Gln Cys Pro Ala Val Leu Glu Glu Ile Asp 705 710 715 720 Gly Gly Ser Ile Trp Arg Ser Phe Ile Thr Gly Leu Asn Thr Met Trp 725 730 735 Asp Phe Cys Lys His Leu Lys Val Ser Phe Gly Leu Asp Gly Ile Val 740 745 750 Val Thr Val Ala Arg Lys Phe Lys Arg Leu Gly Ala Leu Leu Ala Glu 755 760 765 Met Tyr Asn Thr Tyr Leu Ser Thr Val Val Glu Asn Leu Val Leu Ala 770 775 780 Gly Val Ser Phe Lys Tyr Tyr Ala Thr Ser Val Pro Lys Ile Val Leu 785 790 795 800 Gly Cys Cys Phe His Ser Val Lys Ser Val Leu Ala Ser Ala Phe Gln 805 810 815 Ile Pro Val Gln Ala Gly Ile Glu Lys Phe Lys Val Phe Leu Asn Cys 820 825 830 Val His Pro Val Val Pro Arg Val Ile Glu Thr Ser Phe Val Glu Leu 835 840 845 Glu Glu Thr Thr Phe Lys Pro Pro Ala Leu Asn Gly Ser Ile Ala Ile 850 855 860 Val Asp Gly Phe Ala Phe Tyr Tyr Asp Gly Thr Leu Tyr Tyr Pro Thr 865 870 875 880 Asp Gly Asn Ser Val Val Pro Ile Cys Phe Lys Lys Lys Gly Gly Gly 885 890 895 Asp Val Lys Phe Ser Asp Glu Val Ser Val Arg Thr Ile Asp Pro Val 900 905 910 Tyr Lys Val Ser Leu Glu Phe Glu Phe Glu Ser Glu Thr Ile Met Ala 915 920 925 Val Leu Asn Lys Ala Val Gly Asn Arg Ile Lys Val Thr Gly Gly Trp 930 935 940 Asp Asp Val Val Glu Tyr Ile Asn Val Ala Ile Glu Val Leu Lys Asp 945 950 955 960 His Ile Asp Val Pro Lys Tyr Tyr Ile Tyr Asp Glu Glu Gly Gly Thr 965 970 975 Asp Pro Asn Leu Pro Val Met Val Ser Gln Trp Pro Leu Asn Asp Asp 980 985 990 Thr Ile Ser Gln Asp Leu Leu Asp Val Glu Val Val Thr Asp Ala Pro 995 1000 1005 Ile Asp Phe Glu Gly Asp Glu Val Asp Ser Ser Asp Pro Asp Lys Val 1010 1015 1020 Ala Asp Val Ala Asn Ser Glu Pro Glu Asp Asp Gly Pro Asn Val Ala 1025 1030 1035 1040 Pro Glu Thr Asn Val Glu Ser Glu Val Glu Glu Val Ala Ala Thr Leu 1045 1050 1055 Ser Phe Ile Lys Asp Thr Pro Ser Thr Val Thr Lys Asp Pro Phe Ala 1060 1065 1070 Phe Asp Phe Ala Ser Tyr Gly Gly Leu Lys Val Leu Arg Gln Ser His 1075 1080 1085 Asn Asn Cys Trp Val Thr Ser Thr Leu Val Gln Leu Gln Leu Leu Gly 1090 1095 1100 Ile Val Asp Asp Pro Ala Met Glu Leu Phe Ser Ala Gly Arg Val Gly 1105 1110 1115 1120 Pro Met Val Arg Lys Cys Tyr Glu Ser Gln Lys Ala Ile Leu Gly Ser 1125 1130 1135 Leu Gly Asp Val Ser Ala Cys Leu Glu Ser Leu Thr Lys Asp Leu His 1140 1145 1150 Thr Leu Lys Ile Thr Cys Ser Val Val Cys Gly Cys Gly Thr Gly Glu 1155 1160 1165 Arg Ile Tyr Glu Gly Cys Ala Phe Arg Met Thr Pro Thr Leu Glu Pro 1170 1175 1180 Phe Pro Tyr Gly Ala Cys Ala Gln Cys Ala Gln Val Leu Met His Thr 1185 1190 1195 1200 Phe Lys Ser Ile Val Gly Thr Gly Ile Phe Cys Arg Asp Thr Thr Ala 1205 1210 1215 Leu Ser Leu Asp Ser Leu Val Val Lys Pro Leu Cys Ala Ala Ala Phe 1220 1225 1230 Ile Gly Lys Asp Ser Gly His Tyr Val Thr Asn Phe Tyr Asp Ala Ala 1235 1240 1245 Met Ala Ile Asp Gly Tyr Gly Arg His Gln Ile Lys Tyr Asp Thr Leu 1250 1255 1260 Asn Thr Ile Cys Val Lys Asp Val Asn Trp Thr Ala Pro Phe Val Pro 1265 1270 1275 1280 Asp Val Glu Pro Val Leu Glu Pro Val Val Lys Pro Phe Tyr Ser Tyr 1285 1290 1295 Lys Asn Val Asp Phe Tyr Gln Gly Asp Phe Ser Asp Leu Val Lys Leu 1300 1305 1310 Pro Cys Asp Phe Val Val Asn Ala Ala Asn Glu Asn Leu Ser His Gly 1315 1320 1325 Gly Gly Ile Ala Lys Ala Ile Asp Val Tyr Thr Lys Gly Met Leu Gln 1330 1335 1340 Lys Cys Ser Asn Asp Tyr Ile Lys Ala His Gly Pro Ile Lys Val Gly 1345 1350 1355 1360 Arg Gly Val Met Leu Glu Ala Leu Gly Leu Lys Val Phe Asn Val Val 1365 1370 1375 Gly Pro Arg Lys Gly Lys His Ala Pro Glu Leu Leu Val Lys Ala Tyr 1380 1385 1390 Lys Ser Val Phe Ala Asn Ser Gly Val Ala Leu Thr Pro Leu Ile Ser 1395 1400 1405 Val Gly Ile Phe Ser Val Pro Leu Glu Glu Ser Leu Ser Ala Phe Leu 1410 1415 1420 Ala Cys Val Gly Asp Arg His Cys Lys Cys Phe Cys Tyr Ser Asp Lys 1425 1430 1435 1440 Glu Arg Glu Ala Ile Ile Asn Tyr Met Asp Gly Leu Val Asp Ala Ile 1445 1450 1455 Phe Lys Asp Ala Leu Val Asp Thr Thr Pro Val Gln Glu Asp Val Gln 1460 1465 1470 Gln Val Ser Gln Lys Pro Val Leu Pro Asn Phe Glu Pro Phe Arg Ile 1475 1480 1485 Glu Gly Ala His Ala Phe Tyr Glu Cys Asn Pro Glu Gly Leu Met Ser 1490 1495 1500 Leu Gly Ala Asp Lys Leu Val Leu Phe Thr Asn Ser Asn Leu Asp Phe 1505 1510 1515 1520 Cys Ser Val Gly Lys Cys Leu Asn Asn Val Thr Gly Gly Ala Leu Leu 1525 1530 1535 Glu Ala Ile Asn Val Phe Lys Lys Ser Asn Lys Thr Val Pro Ala Gly 1540 1545 1550 Asn Cys Val Thr Phe Glu Cys Ala Asp Met Ile Ser Ile Thr Met Val 1555 1560 1565 Val Leu Pro Ser Asp Gly Asp Ala Asn Tyr Asp Lys Asn Tyr Ala Arg 1570 1575 1580 Ala Val Val Lys Val Ser Lys Leu Lys Gly Lys Leu Leu Leu Ala Val 1585 1590 1595 1600 Gly Asp Ala Met Leu Tyr Ser Lys Leu Ser His Leu Ser Val Leu Gly 1605 1610 1615 Phe Val Ser Thr Pro Asp Asp Val Glu Arg Phe Tyr Ala Asn Lys Ser 1620 1625 1630 Val Val Ile Lys Val Thr Glu Asp Thr Arg Ser Val Lys Thr Val Lys 1635 1640 1645 Val Glu Ser Thr Val Thr Tyr Gly Gln Gln Ile Gly Pro Cys Leu Val 1650 1655 1660 Asn Asp Thr Val Val Thr Asp Asn Lys Pro Val Val Ala Asp Val Val 1665 1670 1675 1680 Ala Lys Val Val Pro Ser Ala Asn Trp Asp Ser His Tyr Gly Phe Asp 1685 1690 1695 Lys Ala Gly Glu Phe His Met Leu Asp His Thr Gly Phe Ala Phe Pro 1700 1705 1710 Ser Glu Val Val Asn Gly Arg Arg Val Leu Lys Thr Thr Asp Asn Asn 1715 1720 1725 Cys Trp Val Asn Val Thr Cys Leu Gln Leu Gln Phe Ala Arg Phe Arg 1730 1735 1740 Phe Lys Ser Ala Gly Leu Gln Ala Met Trp Glu Ser Tyr Cys Thr Gly 1745 1750 1755 1760 Asp Val Ala Met Phe Val His Trp Leu Tyr Trp Leu Thr Gly Val Asp 1765 1770 1775 Lys Gly Gln Pro Ser Asp Ser Glu Asn Ala Leu Asn Met Leu Ser Lys 1780 1785 1790 Tyr Ile Val Pro Ala Gly Ser Val Thr Ile Glu Arg Val Thr His Asp 1795 1800 1805 Gly Cys Cys Cys Ser Lys Arg Val Val Thr Ala Pro Val Val Asn Ala 1810 1815 1820 Ser Val Leu Lys Leu Gly Val Glu Asp Gly Leu Cys Pro His Gly Leu 1825 1830 1835 1840 Asn Tyr Ile Asp Lys Val Val Val Val Lys Gly Thr Thr Ile Val Val 1845 1850 1855 Asn Val Gly Lys Pro Val Val Ala Pro Ser His Leu Phe Leu Lys Gly 1860 1865 1870 Val Ser Tyr Thr Thr Phe Leu Asp Asn Gly Asn Gly Val Ala Gly His 1875 1880 1885 Tyr Thr Val Phe Asp His Asp Thr Gly Met Val His Asp Gly Asp Val 1890 1895 1900 Phe Val Pro Gly Asp Leu Asn Val Ser Pro Val Thr Asn Val Val Val 1905 1910 1915 1920 Ser Glu Gln Thr Ala Val Val Ile Lys Asp Pro Val Lys Lys Val Glu 1925 1930 1935 Leu Asp Ala Thr Lys Leu Leu Asp Thr Met Asn Tyr Ala Ser Glu Arg 1940 1945 1950 Phe Phe Ser Phe Gly Asp Phe Met Ser Arg Asn Leu Ile Thr Val Phe 1955 1960 1965 Leu Tyr Ile Leu Ser Ile Leu Gly Leu Cys Phe Arg Ala Phe Arg Lys 1970 1975 1980 Arg Asp Val Lys Val Leu Ala Gly Val Pro Gln Arg Thr Gly Ile Ile 1985 1990 1995 2000 Leu Arg Lys Ser Val Arg Tyr Asn Ala Lys Ala Leu Gly Val Phe Phe 2005 2010 2015 Lys Leu Lys Leu Tyr Trp Phe Lys Val Leu Gly Lys Phe Ser Leu Gly 2020 2025 2030 Ile Tyr Ala Leu Tyr Ala Leu Leu Phe Met Thr Ile Arg Phe Thr Pro 2035 2040 2045 Ile Gly Gly Pro Val Cys Asp Asp Val Val Ala Gly Tyr Ala Asn Ser 2050 2055 2060 Ser Phe Asp Lys Asn Glu Tyr Cys Asn Ser Val Ile Cys Lys Val Cys 2065 2070 2075 2080 Leu Tyr Gly Tyr Gln Glu Leu Ser Asp Phe Ser His Thr Gln Val Val 2085 2090 2095 Trp Gln His Leu Arg Asp Pro Leu Ile Gly Asn Val Met Pro Phe Phe 2100 2105 2110 Tyr Leu Ala Phe Leu Ala Ile Phe Gly Gly Val Tyr Val Lys Ala Ile 2115 2120 2125 Thr Leu Tyr Phe Ile Phe Gln Tyr Leu Asn Ile Leu Gly Val Phe Leu 2130 2135 2140 Gly Leu Gln Gln Ser Ile Trp Phe Leu Gln Leu Val Pro Phe Asp Val 2145 2150 2155 2160 Phe Gly Asp Glu Ile Val Val Phe Phe Ile Val Thr Arg Val Leu Met 2165 2170 2175 Phe Leu Lys His Val Phe Leu Gly Cys Asp Lys Ala Ser Cys Val Ala 2180 2185 2190 Cys Ser Lys Ser Ala Arg Leu Lys Arg Val Pro Val Gln Thr Ile Phe 2195 2200 2205 Gln Gly Thr Ser Lys Ser Phe Tyr Val His Ala Asn Gly Gly Ser Lys 2210 2215 2220 Phe Cys Lys Lys His Asn Phe Phe Cys Leu Asn Cys Asp Ser Tyr Gly 2225 2230 2235 2240 Pro Gly Cys Thr Phe Ile Asn Asp Val Ile Ala Thr Glu Val Gly Asn 2245 2250 2255 Val Val Lys Leu Asn Val Gln Pro Thr Gly Pro Ala Thr Ile Leu Ile 2260 2265 2270 Asp Lys Val Glu Phe Ser Asn Gly Phe Tyr Tyr Leu Tyr Ser Gly Asp 2275 2280 2285 Thr Phe Trp Lys Tyr Asn Phe Asp Ile Thr Asp Asn Lys Tyr Thr Cys 2290 2295 2300 Lys Glu Ser Leu Lys Asn Cys Ser Ile Ile Thr Asp Phe Ile Val Phe 2305 2310 2315 2320 Asn Asn Asn Gly Ser Asn Val Asn Gln Val Lys Asn Ala Cys Val Tyr 2325 2330 2335 Phe Ser Gln Met Leu Cys Lys Pro Val Lys Leu Val Asp Ser Ala Leu 2340 2345 2350 Leu Ala Ser Leu Ser Val Asp Phe Gly Ala Ser Leu His Ser Ala Phe 2355 2360 2365 Val Ser Val Leu Ser Asn Ser Phe Gly Lys Asp Leu Ser Ser Cys Asn 2370 2375 2380 Asp Met Gln Asp Cys Lys Ser Thr Leu Gly Phe Asp Asp Val Pro Leu 2385 2390 2395 2400 Asp Thr Phe Asn Ala Ala Val Ala Glu Ala His Arg Tyr Asp Val Leu 2405 2410 2415 Leu Thr Asp Met Ser Phe Asn Asn Phe Thr Thr Ser Tyr Ala Lys Pro 2420 2425 2430 Glu Glu Lys Leu Pro Val His Asp Ile Ala Thr Cys Met Arg Val Gly 2435 2440 2445 Ala Lys Ile Val Asn His Asn Val Leu Val Lys Asp Ser Ile Pro Val 2450 2455 2460 Val Trp Leu Val Arg Asp Phe Ile Val Leu Ser Glu Glu Thr Arg Lys 2465 2470 2475 2480 Tyr Ile Ile Arg Thr Thr Lys Val Lys Gly Ile Thr Phe Met Leu Thr 2485 2490 2495 Phe Asn Asp Cys Arg Met His Thr Thr Ile Pro Thr Val Cys Ile Ala 2500 2505 2510 Asn Lys Lys Gly Ala Gly Leu Pro Ser Phe Ser Lys Val Lys Lys Phe 2515 2520 2525 Phe Trp Phe Leu Cys Leu Phe Ile Val Ala Val Phe Phe Ala Leu Ser 2530 2535 2540 Phe Phe Asp Phe Ser Thr Gln Val Ser Ser Asp Ser Asp Tyr Asp Phe 2545 2550 2555 2560 Lys Tyr Ile Glu Ser Gly Gln Leu Lys Thr Phe Asp Asn Pro Leu Ser 2565 2570 2575 Cys Val His Asn Val Phe Ser Asn Phe Asp Gln Trp His Asp Ala Lys 2580 2585 2590 Phe Gly Phe Thr Pro Val Asn Asn Pro Ser Cys Pro Ile Val Val Gly 2595 2600 2605 Val Ser Asp Glu Ala Arg Thr Val Pro Gly Ile Pro Ala Gly Val Tyr 2610 2615 2620 Leu Ala Gly Lys Thr Leu Val Phe Ala Ile Asn Thr Ile Phe Gly Thr 2625 2630 2635 2640 Ser Gly Leu Cys Phe Asp Ala Ser Gly Val Ala Asp Lys Gly Ala Cys 2645 2650 2655 Ile Phe Asn Ser Ala Cys Thr Thr Leu Ser Gly Leu Gly Gly Thr Ala 2660 2665 2670 Val Tyr Cys Tyr Lys Asn Gly Leu Val Glu Gly Ala Lys Leu Tyr Ser 2675 2680 2685 Glu Leu Ala Pro His Ser Tyr Tyr Lys Met Val Asp Gly Asn Ala Val 2690 2695 2700 Ser Leu Pro Glu Ile Ile Ser Arg Gly Phe Gly Ile Arg Thr Ile Arg 2705 2710 2715 2720 Thr Lys Ala Met Thr Tyr Cys Arg Val Gly Gln Cys Val Gln Ser Ala 2725 2730 2735 Glu Gly Val Cys Phe Gly Ala Asp Arg Phe Phe Val Tyr Asn Ala Glu 2740 2745 2750 Ser Gly Ser Asp Phe Val Cys Gly Thr Gly Leu Phe Thr Leu Leu Met 2755 2760 2765 Asn Val Ile Ser Val Phe Ser Lys Thr Val Pro Val Thr Val Leu Ser 2770 2775 2780 Gly Gln Ile Leu Phe Asn Cys Ile Ile Ala Phe Ala Ala Val Ala Val 2785 2790 2795 2800 Cys Phe Leu Phe Thr Lys Phe Lys Arg Met Phe Gly Asp Met Ser Val 2805 2810 2815 Gly Val Phe Thr Val Gly Ala Cys Thr Leu Leu Asn Asn Val Ser Tyr 2820 2825 2830 Ile Val Thr Gln Asn Thr Leu Gly Met Leu Gly Tyr Ala Thr Leu Tyr 2835 2840 2845 Phe Leu Cys Thr Lys Gly Val Arg Tyr Met Trp Ile Trp His Leu Gly 2850 2855 2860 Phe Leu Ile Ser Tyr Ile Leu Ile Ala Pro Trp Trp Val Leu Met Val 2865 2870 2875 2880 Tyr Ala Phe Ser Ala Ile Phe Glu Phe Met Pro Asn Leu Phe Lys Leu 2885 2890 2895 Lys Val Ser Thr Gln Leu Phe Glu Gly Asp Lys Phe Val Gly Ser Phe 2900 2905 2910 Glu Asn Ala Ala Ala Gly Thr Phe Val Leu Asp Met His Ala Tyr Glu 2915 2920 2925 Arg Leu Ala Asn Ser Ile Ser Thr Glu Lys Leu Arg Gln Tyr Ala Ser 2930 2935 2940 Thr Tyr Asn Lys Tyr Lys Tyr Tyr Ser Gly Ser Ala Ser Glu Ala Asp 2945 2950 2955 2960 Tyr Arg Leu Ala Cys Phe Ala His Leu Ala Lys Ala Met Met Asp Tyr 2965 2970 2975 Ala Ser Asn His Asn Asp Thr Leu Tyr Thr Pro Pro Thr Val Ser Tyr 2980 2985 2990 Asn Ser Thr Leu Gln Ala Gly Leu Arg Lys Met Ala Gln Pro Ser Gly 2995 3000 3005 Val Val Glu Lys Cys Ile Val Arg Val Cys Tyr Gly Asn Met Ala Leu 3010 3015 3020 Asn Gly Leu Trp Leu Gly Asp Thr Val Ile Cys Pro Arg His Val Ile 3025 3030 3035 3040 Ala Ser Ser Thr Thr Ser Thr Ile Asp Tyr Asp Tyr Ala Leu Ser Val 3045 3050 3055 Leu Arg Leu His Asn Phe Ser Ile Ser Ser Gly Asn Val Phe Leu Gly 3060 3065 3070 Val Val Gly Val Thr Met Arg Gly Ala Leu Leu Gln Ile Lys Val Asn 3075 3080 3085 Gln Asn Asn Val His Thr Pro Lys Tyr Thr Tyr Arg Thr Val Arg Pro 3090 3095 3100 Gly Glu Ser Phe Asn Ile Leu Ala Cys Tyr Asp Gly Ser Ala Ala Gly 3105 3110 3115 3120 Val Tyr Gly Val Asn Met Arg Ser Asn Tyr Thr Ile Arg Gly Ser Phe 3125 3130 3135 Ile Asn Gly Ala Cys Gly Ser Pro Gly Tyr Asn Ile Asn Asn Gly Thr 3140 3145 3150 Val Glu Phe Cys Tyr Leu His Gln Leu Glu Leu Gly Ser Gly Cys His 3155 3160 3165 Val Gly Ser Asp Leu Asp Gly Val Met Tyr Gly Gly Tyr Glu Asp Gln 3170 3175 3180 Pro Thr Leu Gln Val Glu Gly Ala Ser Ser Leu Phe Thr Glu Asn Val 3185 3190 3195 3200 Leu Ala Phe Leu Tyr Ala Ala Leu Ile Asn Gly Ser Thr Trp Trp Leu 3205 3210 3215 Ser Ser Ser Arg Ile Ala Val Asp Arg Phe Asn Glu Trp Ala Val His 3220 3225 3230 Asn Gly Met Thr Thr Val Val Asn Thr Asp Cys Phe Ser Ile Leu Ala 3235 3240 3245 Ala Lys Thr Gly Val Asp Val Gln Arg Leu Leu Ala Ser Ile Gln Ser 3250 3255 3260 Leu His Lys Asn Phe Gly Gly Lys Gln Ile Leu Gly Tyr Thr Ser Leu 3265 3270 3275 3280 Thr Asp Glu Phe Thr Thr Gly Glu Val Ile Arg Gln Met Tyr Gly Val 3285 3290 3295 Asn Leu Gln Ser Gly Tyr Val Ser Arg Ala Cys Arg Asn Val Leu Leu 3300 3305 3310 Val Gly Ser Phe Leu Thr Phe Phe Trp Ser Glu Leu Val Ser Tyr Thr 3315 3320 3325 Lys Phe Phe Trp Val Asn Pro Gly Tyr Val Thr Pro Met Phe Ala Cys 3330 3335 3340 Leu Ser Leu Leu Ser Ser Leu Leu Met Phe Thr Leu Lys His Lys Thr 3345 3350 3355 3360 Leu Phe Phe Gln Val Phe Leu Ile Pro Ala Leu Ile Val Thr Ser Cys 3365 3370 3375 Ile Asn Leu Ala Phe Asp Val Glu Val Tyr Asn Tyr Leu Ala Glu His 3380 3385 3390 Phe Asp Tyr His Val Ser Leu Met Gly Phe Asn Ala Gln Gly Leu Val 3395 3400 3405 Asn Ile Phe Val Cys Phe Val Val Thr Ile Leu His Gly Thr Tyr Thr 3410 3415 3420 Trp Arg Phe Phe Asn Thr Pro Val Ser Ser Val Thr Tyr Val Val Ala 3425 3430 3435 3440 Leu Leu Thr Ala Ala Tyr Asn Tyr Phe Tyr Ala Ser Asp Ile Leu Ser 3445 3450 3455 Cys Ala Met Thr Leu Phe Ala Ser Val Thr Gly Asn Trp Phe Val Gly 3460 3465 3470 Ala Val Cys Tyr Lys Ala Ala Val Tyr Met Ala Leu Arg Phe Pro Thr 3475 3480 3485 Phe Val Ala Ile Phe Gly Asp Ile Lys Ser Val Met Phe Cys Tyr Leu 3490 3495 3500 Val Leu Gly Tyr Phe Thr Cys Cys Phe Tyr Gly Ile Leu Tyr Trp Phe 3505 3510 3515 3520 Asn Arg Phe Phe Lys Val Ser Val Gly Val Tyr Asp Tyr Thr Val Ser 3525 3530 3535 Ala Ala Glu Phe Lys Tyr Met Val Ala Asn Gly Leu Arg Ala Pro Thr 3540 3545 3550 Gly Thr Leu Asp Ser Leu Leu Leu Ser Ala Lys Leu Ile Gly Ile Gly 3555 3560 3565 Gly Glu Arg Asn Ile Lys Ile Ser Ser Val Gln Ser Lys Leu Thr Asp 3570 3575 3580 Ile Lys Cys Ser Asn Val Val Leu Leu Gly Cys Leu Ser Ser Met Asn 3585 3590 3595 3600 Val Ser Ala Asn Ser Thr Glu Trp Ala Tyr Cys Val Asp Leu His Asn 3605 3610 3615 Lys Ile Asn Leu Cys Asn Asp Pro Glu Lys Ala Gln Glu Met Leu Leu 3620 3625 3630 Ala Leu Leu Ala Phe Phe Leu Ser Lys Asn Ser Ala Phe Gly Leu Asp 3635 3640 3645 Asp Leu Leu Glu Ser Tyr Phe Asn Asp Asn Ser Met Leu Gln Ser Val 3650 3655 3660 Ala Ser Thr Tyr Val Gly Leu Pro Ser Tyr Val Ile Tyr Glu Asn Ala 3665 3670 3675 3680 Arg Gln Gln Tyr Glu Asp Ala Val Asn Asn Gly Ser Pro Pro Gln Leu 3685 3690 3695 Val Lys Gln Leu Arg His Ala Met Asn Val Ala Lys Ser Glu Phe Asp 3700 3705 3710 Arg Glu Ala Ser Thr Gln Arg Lys Leu Asp Arg Met Ala Glu Gln Ala 3715 3720 3725 Ala Ala Gln Met Tyr Lys Glu Ala Arg Ala Val Asn Arg Lys Ser Lys 3730 3735 3740 Val Val Ser Ala Met His Ser Leu Leu Phe Gly Met Leu Arg Arg Leu 3745 3750 3755 3760 Asp Met Ser Ser Val Asp Thr Ile Leu Asn Leu Ala Lys Asp Gly Val 3765 3770 3775 Val Pro Leu Ser Val Ile Pro Ala Val Ser Ala Thr Lys Leu Asn Ile 3780 3785 3790 Val Thr Ser Asp Ile Asp Ser Tyr Asn Arg Ile Gln Arg Glu Gly Cys 3795 3800 3805 Val His Tyr Ala Gly Thr Ile Trp Asn Ile Ile Asp Ile Lys Asp Asn 3810 3815 3820 Asp Gly Lys Val Val His Val Lys Glu Val Thr Ala Gln Asn Ala Glu 3825 3830 3835 3840 Ser Leu Ser Trp Pro Leu Val Leu Gly Cys Glu Arg Ile Val Lys Leu 3845 3850 3855 Gln Asn Asn Glu Ile Ile Pro Gly Lys Leu Lys Gln Arg Ser Ile Lys 3860 3865 3870 Ala Glu Gly Asp Gly Ile Val Gly Glu Gly Lys Ala Leu Tyr Asn Asn 3875 3880 3885 Glu Gly Gly Arg Thr Phe Met Tyr Ala Phe Ile Ser Asp Lys Pro Asp 3890 3895 3900 Leu Arg Val Val Lys Trp Glu Phe Asp Gly Gly Cys Asn Thr Ile Glu 3905 3910 3915 3920 Leu Glu Pro Pro Arg Lys Phe Leu Val Asp Ser Pro Asn Gly Ala Gln 3925 3930 3935 Ile Lys Tyr Leu Tyr Phe Val Arg Asn Leu Asn Thr Leu Arg Arg Gly 3940 3945 3950 Ala Val Leu Gly Tyr Ile Gly Ala Thr Val Arg Leu Gln Ala Gly Lys 3955 3960 3965 Gln Thr Glu Gln Ala Ile Asn Ser Ser Leu Leu Thr Leu Cys Ala Phe 3970 3975 3980 Ala Val Asp Pro Ala Lys Thr Tyr Ile Asp Ala Val Lys Ser Gly His 3985 3990 3995 4000 Lys Pro Val Gly Asn Cys Val Lys Met Leu Ala Asn Gly Ser Gly Asn 4005 4010 4015 Gly Gln Ala Val Thr Asn Gly Val Glu Ala Ser Thr Asn Gln Asp Ser 4020 4025 4030 Tyr Gly Gly Ala Ser Val Cys Leu Tyr Cys Arg Ala His Val Glu His 4035 4040 4045 Pro Ser Met Asp Gly Phe Cys Arg Leu Lys Gly Lys Tyr Val Gln Val 4050 4055 4060 Pro Leu Gly Thr Val Asp Pro Ile Arg Phe Val Leu Glu Asn Asp Val 4065 4070 4075 4080 Cys Lys Val Cys Gly Cys Trp Leu Ala Asn Gly Cys Thr Cys Asp Arg 4085 4090 4095 Ser Ile Met Gln Ser Thr Asp Tyr Gly Leu Phe Lys Arg Val Arg Gly 4100 4105 4110 Ser Ser Ala Ala Arg Leu Glu Pro Cys Asn Gly Thr Asp Thr Gln His 4115 4120 4125 Val Tyr Arg Ala Phe Asp Ile Tyr Asn Lys Asp Val Ala Cys Leu Gly 4130 4135 4140 Lys Phe Leu Lys Val Asn Cys Val Arg Leu Lys Asn Leu Asp Lys His 4145 4150 4155 4160 Asp Ala Phe Tyr Val Val Lys Arg Cys Thr Lys Ser Ala Met Glu His 4165 4170 4175 Glu Gln Ser Ile Tyr Ser Arg Leu Glu Lys Cys Gly Ala Val Ala Glu 4180 4185 4190 His Asp Phe Phe Thr Trp Lys Asp Gly Arg Ala Ile Tyr Gly Asn Val 4195 4200 4205 Cys Arg Lys Asp Leu Thr Glu Tyr Thr Met Met Asp Leu Cys Tyr Ala 4210 4215 4220 Leu Arg Asn Phe Asp Glu Asn Asn Cys Asp Val Leu Lys Ser Ile Leu 4225 4230 4235 4240 Ile Lys Val Gly Ala Cys Glu Glu Ser Tyr Phe Asn Asn Lys Val Trp 4245 4250 4255 Phe Asp Pro Val Glu Asn Glu Asp Ile His Arg Val Tyr Ala Leu Leu 4260 4265 4270 Gly Thr Ile Val Ser Arg Ala Met Leu Lys Cys Val Lys Phe Cys Asp 4275 4280 4285 Ala Met Val Glu Gln Gly Ile Val Gly Val Val Thr Leu Asp Asn Gln 4290 4295 4300 Asp Leu Asn Gly Asp Phe Tyr Asp Phe Gly Asp Phe Thr Cys Ser Ile 4305 4310 4315 4320 Lys Gly Met Gly Ile Pro Ile Cys Thr Ser Tyr Tyr Ser Tyr Met Met 4325 4330 4335 Pro Val Met Gly Met Thr Asn Cys Leu Ala Ser Glu Cys Phe Val Lys 4340 4345 4350 Ser Asp Ile Phe Gly Glu Asp Phe Lys Ser Tyr Asp Leu Leu Glu Tyr 4355 4360 4365 Asp Phe Thr Glu His Lys Thr Ala Leu Phe Asn Lys Tyr Phe Lys Tyr 4370 4375 4380 Trp Gly Leu Gln Tyr His Pro Asn Cys Val Asp Cys Ser Asp Glu Gln 4385 4390 4395 4400 Cys Ile Val His Cys Ala Asn Phe Asn Thr Leu Phe Ser Thr Thr Ile 4405 4410 4415 Pro Ile Thr Ala Phe Gly Pro Leu Cys Arg Lys Cys Trp Ile Asp Gly 4420 4425 4430 Val Pro Leu Val Thr Thr Ala Gly Tyr His Phe Lys Gln Leu Gly Ile 4435 4440 4445 Val Trp Asn Asn Asp Leu Asn Leu His Ser Ser Arg Leu Ser Ile Asn 4450 4455 4460 Glu Leu Leu Gln Phe Cys Ser Asp Pro Ala Leu Leu Ile Ala Ser Ser 4465 4470 4475 4480 Pro Ala Leu Val Asp Gln Arg Thr Val Cys Phe Ser Val Ala Ala Leu 4485 4490 4495 Gly Thr Gly Met Thr Asn Gln Thr Val Lys Pro Gly His Phe Asn Lys 4500 4505 4510 Glu Phe Tyr Asp Phe Leu Leu Glu Gln Gly Phe Phe Ser Glu Gly Ser 4515 4520 4525 Glu Leu Thr Leu Lys His Phe Phe Phe Ala Gln Lys Gly Asp Ala Ala 4530 4535 4540 Val Lys Asp Phe Asp Tyr Tyr Arg Tyr Asn Arg Pro Thr Val Leu Asp 4545 4550 4555 4560 Ile Cys Gln Ala Arg Val Val Tyr Gln Ile Val Gln Arg Tyr Phe Asp 4565 4570 4575 Ile Tyr Glu Gly Gly Cys Ile Thr Ala Lys Glu Val Val Val Thr Asn 4580 4585 4590 Leu Asn Lys Ser Ala Gly Tyr Pro Leu Asn Lys Phe Gly Lys Ala Gly 4595 4600 4605 Leu Tyr Tyr Glu Ser Leu Ser Tyr Glu Glu Gln Asp Glu Leu Tyr Ala 4610 4615 4620 Tyr Thr Lys Arg Asn Ile Leu Pro Thr Met Thr Gln Leu Asn Leu Lys 4625 4630 4635 4640 Tyr Ala Ile Ser Gly Lys Glu Arg Ala Arg Thr Val Gly Gly Val Ser 4645 4650 4655 Leu Leu Ser Thr Met Thr Thr Arg Gln Tyr His Gln Lys His Leu Lys 4660 4665 4670 Ser Ile Val Asn Thr Arg Gly Ala Ser Val Val Ile Gly Thr Thr Lys 4675 4680 4685 Phe Tyr Gly Gly Trp Asp Asn Met Leu Lys Asn Leu Ile Asp Gly Val 4690 4695 4700 Glu Asn Pro Cys Leu Met Gly Trp Asp Tyr Pro Lys Cys Asp Arg Ala 4705 4710 4715 4720 Leu Pro Asn Met Ile Arg Met Ile Ser Ala Met Ile Leu Gly Ser Lys 4725 4730 4735 His Thr Thr Cys Cys Ser Ser Thr Asp Arg Phe Phe Arg Leu Cys Asn 4740 4745 4750 Glu Leu Ala Gln Val Leu Thr Glu Val Val Tyr Ser Asn Gly Gly Phe 4755 4760 4765 Tyr Leu Lys Pro Gly Gly Thr Thr Ser Gly Asp Ala Thr Thr Ala Tyr 4770 4775 4780 Ala Asn Ser Val Phe Asn Ile Phe Gln Ala Val Ser Ala Asn Val Asn 4785 4790 4795 4800 Lys Leu Leu Ser Val Asp Ser Asn Val Cys His Asn Leu Glu Val Lys 4805 4810 4815 Gln Leu Gln Arg Lys Leu Tyr Glu Cys Cys Tyr Arg Ser Thr Thr Val 4820 4825 4830 Asp Asp Gln Phe Val Val Glu Tyr Tyr Gly Tyr Leu Arg Lys His Phe 4835 4840 4845 Ser Met Met Ile Leu Ser Asp Asp Gly Val Val Cys Tyr Asn Asn Asp 4850 4855 4860 Tyr Ala Ser Leu Gly Tyr Val Ala Asp Leu Asn Ala Phe Lys Ala Val 4865 4870 4875 4880 Leu Tyr Tyr Gln Asn Asn Val Phe Met Ser Ala Ser Lys Cys Trp Ile 4885 4890 4895 Glu Pro Asp Ile Asn Lys Gly Pro His Glu Phe Cys Ser Gln His Thr 4900 4905 4910 Met Gln Ile Val Asp Lys Asp Gly Thr Tyr Tyr Leu Pro Tyr Pro Asp 4915 4920 4925 Pro Ser Arg Ile Leu Ser Ala Gly Val Phe Val Asp Asp Val Val Lys 4930 4935 4940 Thr Asp Ala Val Val Leu Leu Glu Arg Tyr Val Ser Leu Ala Ile Asp 4945 4950 4955 4960 Ala Tyr Pro Leu Ser Lys His Glu Asn Pro Glu Tyr Lys Lys Val Phe 4965 4970 4975 Tyr Val Leu Leu Asp Trp Val Lys His Leu Tyr Lys Thr Leu Asn Ala 4980 4985 4990 Gly Val Leu Glu Ser Phe Ser Val Thr Leu Leu Glu Asp Ser Thr Ala 4995 5000 5005 Lys Phe Trp Asp Glu Ser Phe Tyr Ala Asn Met Tyr Glu Lys Ser Ala 5010 5015 5020 Val Leu Gln Ser Ala Gly Leu Cys Val Val Cys Gly Ser Gln Thr Val 5025 5030 5035 5040 Leu Arg Cys Gly Asp Cys Leu Arg Arg Pro Met Leu Cys Thr Lys Cys 5045 5050 5055 Ala Tyr Asp His Val Ile Gly Thr Thr His Lys Phe Ile Leu Ala Ile 5060 5065 5070 Thr Pro Tyr Val Cys Cys Ala Ser Asp Cys Gly Val Asn Asp Val Thr 5075 5080 5085 Lys Leu Tyr Leu Gly Gly Leu Ser Tyr Trp Cys His Asp His Lys Pro 5090 5095 5100 Arg Leu Ala Phe Pro Leu Cys Ser Ala Gly Asn Val Phe Gly Leu Tyr 5105 5110 5115 5120 Lys Asn Ser Ala Thr Gly Ser Pro Asp Val Glu Asp Phe Asn Arg Ile 5125 5130 5135 Ala Thr Ser Asp Trp Thr Asp Val Ser Asp Tyr Arg Leu Ala Asn Asp 5140 5145 5150 Val Lys Asp Ser Leu Arg Leu Phe Ala Ala Glu Thr Ile Lys Ala Lys 5155 5160 5165 Glu Glu Ser Val Lys Ser Ser Tyr Ala Cys Ala Thr Leu His Glu Val 5170 5175 5180 Val Gly Pro Lys Glu Leu Leu Leu Lys Trp Glu Val Gly Arg Pro Lys 5185 5190 5195 5200 Pro Pro Leu Asn Arg Asn Ser Val Phe Thr Cys Tyr His Ile Thr Lys 5205 5210 5215 Asn Thr Lys Phe Gln Ile Gly Glu Phe Val Phe Glu Lys Ala Glu Tyr 5220 5225 5230 Asp Asn Asp Ala Val Thr Tyr Lys Thr Thr Ala Thr Thr Lys Leu Val 5235 5240 5245 Pro Gly Met Val Phe Val Leu Thr Ser His Asn Val Gln Pro Leu Arg 5250 5255 5260 Ala Pro Thr Ile Ala Asn Gln Glu Arg Tyr Ser Thr Ile His Lys Leu 5265 5270 5275 5280 His Pro Ala Phe Asn Ile Pro Glu Ala Tyr Ser Ser Leu Val Pro Tyr 5285 5290 5295 Tyr Gln Leu Ile Gly Lys Gln Lys Ile Thr Thr Ile Gln Gly Pro Pro 5300 5305 5310 Gly Ser Gly Lys Ser His Cys Val Ile Gly Leu Gly Leu Tyr Tyr Pro 5315 5320 5325 Gly Ala Arg Ile Val Phe Thr Ala Cys Ser His Ala Ala Val Asp Ser 5330 5335 5340 Leu Cys Val Lys Ala Ser Thr Ala Tyr Ser Asn Asp Lys Cys Ser Arg 5345 5350 5355 5360 Ile Ile Pro Gln Arg Ala Arg Val Glu Cys Tyr Asp Gly Phe Lys Ser 5365 5370 5375 Asn Asn Thr Ser Ala Gln Tyr Leu Phe Ser Thr Val Asn Ala Leu Pro 5380 5385 5390 Glu Cys Asn Ala Asp Ile Val Val Val Asp Glu Val Ser Met Cys Thr 5395 5400 5405 Asn Tyr Asp Leu Ser Val Ile Asn Gln Arg Ile Ser Tyr Arg His Val 5410 5415 5420 Val Tyr Val Gly Asp Pro Gln Gln Leu Pro Ala Pro Arg Val Met Ile 5425 5430 5435 5440 Ser Arg Gly Thr Leu Glu Pro Lys Asp Tyr Asn Val Val Thr Gln Arg 5445 5450 5455 Met Cys Ala Leu Lys Pro Asp Val Phe Leu His Lys Cys Tyr Arg Cys 5460 5465 5470 Pro Ala Glu Ile Val Arg Thr Val Ser Glu Met Val Tyr Glu Asn Gln 5475 5480 5485 Phe Ile Pro Val His Pro Asp Ser Lys Gln Cys Phe Lys Ile Phe Cys 5490 5495 5500 Lys Gly Asn Val Gln Val Asp Asn Gly Ser Ser Ile Asn Arg Arg Gln 5505 5510 5515 5520 Leu Asp Val Val Arg Met Phe Leu Ala Lys Asn Pro Arg Trp Ser Lys 5525 5530 5535 Ala Val Phe Ile Ser Pro Tyr Asn Ser Gln Asn Tyr Val Ala Ser Arg 5540 5545 5550 Met Leu Gly Leu Gln Ile Gln Thr Val Asp Ser Ser Gln Gly Ser Glu 5555 5560 5565 Tyr Asp Tyr Val Ile Tyr Thr Gln Thr Ser Asp Thr Ala His Ala Cys 5570 5575 5580 Asn Val Asn Arg Phe Asn Val Ala Ile Thr Arg Ala Lys Lys Gly Ile 5585 5590 5595 5600 Leu Cys Ile Met Cys Asp Arg Ser Leu Phe Asp Val Leu Lys Phe Phe 5605 5610 5615 Glu Leu Lys Leu Ser Asp Leu Gln Ala Asn Glu Gly Cys Gly Leu Phe 5620 5625 5630 Lys Asp Cys Ser Arg Gly Asp Asp Leu Leu Pro Pro Ser His Ala Asn 5635 5640 5645 Thr Phe Met Ser Leu Ala Asp Asn Phe Lys Thr Asp Gln Asp Leu Ala 5650 5655 5660 Val Gln Ile Gly Val Asn Gly Pro Ile Lys Tyr Glu His Val Ile Ser 5665 5670 5675 5680 Phe Met Gly Phe Arg Phe Asp Ile Asn Ile Pro Asn His His Thr Leu 5685 5690 5695 Phe Cys Thr Arg Asp Phe Ala Met Arg Asn Val Arg Gly Trp Leu Gly 5700 5705 5710 Phe Asp Val Glu Gly Ala His Val Val Gly Ser Asn Val Gly Thr Asn 5715 5720 5725 Val Pro Leu Gln Leu Gly Phe Ser Asn Gly Val Asp Phe Val Val Arg 5730 5735 5740 Pro Glu Gly Cys Val Val Thr Glu Ser Gly Asp Tyr Ile Lys Pro Val 5745 5750 5755 5760 Arg Ala Arg Ala Pro Pro Gly Glu Gln Phe Ala His Leu Leu Pro Leu 5765 5770 5775 Leu Lys Arg Gly Gln Pro Trp Asp Val Val Arg Lys Arg Ile Val Gln 5780 5785 5790 Met Cys Ser Asp Tyr Leu Ala Asn Leu Ser Asp Ile Leu Ile Phe Val 5795 5800 5805 Leu Trp Ala Gly Gly Leu Glu Leu Thr Thr Met Arg Tyr Phe Val Lys 5810 5815 5820 Ile Gly Pro Ser Lys Ser Cys Asp Cys Gly Lys Val Ala Thr Cys Tyr 5825 5830 5835 5840 Asn Ser Ala Leu His Thr Tyr Cys Cys Phe Lys His Ala Leu Gly Cys 5845 5850 5855 Asp Tyr Leu Tyr Asn Pro Tyr Cys Ile Asp Ile Gln Gln Trp Gly Tyr 5860 5865 5870 Lys Gly Ser Leu Ser Leu Asn His His Glu His Cys Asn Val His Arg 5875 5880 5885 Asn Glu His Val Ala Ser Gly Asp Ala Ile Met Thr Arg Cys Leu Ala 5890 5895 5900 Ile His Asp Cys Phe Val Lys Asn Val Asp Trp Ser Ile Thr Tyr Pro 5905 5910 5915 5920 Phe Ile Gly Asn Glu Ala Val Ile Asn Lys Ser Gly Arg Ile Val Gln 5925 5930 5935 Ser His Thr Met Arg Ser Val Leu Lys Leu Tyr Asn Pro Lys Ala Ile 5940 5945 5950 Tyr Asp Ile Gly Asn Pro Lys Gly Ile Arg Cys Ala Val Thr Asp Ala 5955 5960 5965 Lys Trp Phe Cys Phe Asp Lys Asn Pro Thr Asn Ser Asn Val Lys Thr 5970 5975 5980 Leu Glu Tyr Asp Tyr Ile Thr His Gly Gln Phe Asp Gly Leu Cys Leu 5985 5990 5995 6000 Phe Trp Asn Cys Asn Val Asp Met Tyr Pro Glu Phe Ser Val Val Cys 6005 6010 6015 Arg Phe Asp Thr Arg Cys Arg Ser Pro Leu Asn Leu Glu Gly Cys Asn 6020 6025 6030 Gly Gly Ser Leu Tyr Val Asn Asn His Ala Phe His Thr Pro Ala Phe 6035 6040 6045 Asp Lys Arg Ala Phe Ala Lys Leu Lys Pro Met Pro Phe Phe Phe Tyr 6050 6055 6060 Asp Asp Thr Glu Cys Asp Lys Leu Gln Asp Ser Ile Asn Tyr Val Pro 6065 6070 6075 6080 Leu Arg Ala Ser Asn Cys Ile Thr Lys Cys Asn Val Gly Gly Ala Val 6085 6090 6095 Cys Ser Lys His Cys Ala Met Tyr His Ser Tyr Val Asn Ala Tyr Asn 6100 6105 6110 Thr Phe Thr Ser Ala Gly Phe Thr Ile Trp Val Pro Thr Ser Phe Asp 6115 6120 6125 Thr Tyr Asn Leu Trp Gln Thr Phe Ser Asn Asn Leu Gln Gly Leu Glu 6130 6135 6140 Asn Ile Ala Phe Asn Val Val Lys Lys Gly Ser Phe Val Gly Ala Glu 6145 6150 6155 6160 Gly Glu Leu Pro Val Ala Val Val Asn Asp Lys Val Leu Val Arg Asp 6165 6170 6175 Gly Thr Val Asp Thr Leu Val Phe Thr Asn Lys Thr Ser Leu Pro Thr 6180 6185 6190 Asn Val Ala Phe Glu Leu Tyr Ala Lys Arg Lys Val Gly Leu Thr Pro 6195 6200 6205 Pro Ile Thr Ile Leu Arg Asn Leu Gly Val Val Cys Thr Ser Lys Cys 6210 6215 6220 Val Ile Trp Asp Tyr Glu Ala Glu Arg Pro Leu Thr Thr Phe Thr Lys 6225 6230 6235 6240 Asp Val Cys Lys Tyr Thr Asp Phe Glu Gly Asp Val Cys Thr Leu Phe 6245 6250 6255 Asp Asn Ser Ile Val Gly Ser Leu Glu Arg Phe Ser Met Thr Gln Asn 6260 6265 6270 Ala Val Leu Met Ser Leu Thr Ala Val Lys Lys Leu Thr Gly Ile Lys 6275 6280 6285 Leu Thr Tyr Gly Tyr Leu Asn Gly Val Pro Val Asn Thr His Glu Asp 6290 6295 6300 Lys Pro Phe Thr Trp Tyr Ile Tyr Thr Arg Lys Asn Gly Lys Phe Glu 6305 6310 6315 6320 Asp His Pro Asp Gly Tyr Phe Thr Gln Gly Arg Thr Thr Ala Asp Phe 6325 6330 6335 Ser Pro Arg Ser Asp Met Glu Lys Asp Phe Leu Ser Met Asp Met Gly 6340 6345 6350 Leu Phe Ile Asn Lys Tyr Gly Leu Glu Asp Tyr Gly Phe Glu His Val 6355 6360 6365 Val Tyr Gly Asp Val Ser Lys Thr Thr Leu Gly Gly Leu His Leu Leu 6370 6375 6380 Ile Ser Gln Val Arg Leu Ala Cys Met Gly Val Leu Lys Ile Asp Glu 6385 6390 6395 6400 Phe Val Ser Ser Asn Asp Ser Thr Leu Lys Ser Cys Thr Val Thr Tyr 6405 6410 6415 Ala Asp Asn Pro Ser Ser Lys Met Val Cys Thr Tyr Met Asp Leu Leu 6420 6425 6430 Leu Asp Asp Phe Val Ser Ile Leu Lys Ser Leu Asp Leu Gly Val Val 6435 6440 6445 Ser Lys Val His Glu Val Met Val Asp Cys Lys Met Trp Arg Trp Met 6450 6455 6460 Leu Trp Cys Lys Asp His Lys Leu Gln Thr Phe Tyr Pro Gln Leu Gln 6465 6470 6475 6480 Ala Ser Glu Trp Lys Cys Gly Tyr Ser Met Pro Ser Ile Tyr Lys Ile 6485 6490 6495 Gln Arg Met Cys Leu Glu Pro Cys Asn Leu Tyr Asn Tyr Gly Ala Gly 6500 6505 6510 Ile Lys Leu Pro Asp Gly Ile Met Phe Asn Val Val Lys Tyr Thr Gln 6515 6520 6525 Leu Cys Gln Tyr Leu Asn Ser Thr Thr Met Cys Val Pro His His Met 6530 6535 6540 Arg Val Leu His Leu Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly 6545 6550 6555 6560 Thr Ala Val Leu Arg Arg Trp Leu Pro Leu Asp Ala Ile Ile Val Asp 6565 6570 6575 Asn Asp Ser Val Asp Tyr Val Ser Asp Ala Asp Tyr Ser Val Thr Gly 6580 6585 6590 Asp Cys Ser Thr Leu Tyr Leu Ser Asp Lys Phe Asp Leu Val Ile Ser 6595 6600 6605 Asp Met Tyr Asp Gly Lys Ile Lys Ser Cys Asp Gly Glu Asn Val Ser 6610 6615 6620 Lys Glu Gly Phe Phe Pro Tyr Ile Asn Gly Val Ile Thr Glu Lys Leu 6625 6630 6635 6640 Ala Leu Gly Gly Thr Val Ala Ile Lys Val Thr Glu Phe Ser Trp Asn 6645 6650 6655 Lys Lys Leu Tyr Glu Leu Ile Gln Arg Phe Glu Tyr Trp Thr Met Phe 6660 6665 6670 Cys Thr Ser Val Asn Thr Ser Ser Ser Glu Ala Phe Leu Ile Gly Val 6675 6680 6685 His Tyr Leu Gly Asp Phe Ala Ser Gly Ala Val Ile Asp Gly Asn Thr 6690 6695 6700 Met His Ala Asn Tyr Ile Phe Trp Arg Asn Ser Thr Ile Met Thr Met 6705 6710 6715 6720 Ser Tyr Asn Ser Val Leu Asp Leu Ser Lys Phe Asn Cys Lys His Lys 6725 6730 6735 Ala Thr Val Val Ile Asn Leu Lys Asp Ser Ser Ile Ser Asp Val Val 6740 6745 6750 Leu Gly Leu Leu Lys Asn Gly Lys Leu Leu Val Arg Asn Asn Asp Ala 6755 6760 6765 Ile Cys Gly Phe Ser Asn His Leu Val Asn Val Asn Lys 6770 6775 6780 <210> 10 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 ORF3 <400> 10 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Asp Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 11 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 envelope protein <400> 11 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Pro Ser Thr Val Ile Asp Val 65 70 75 <210> 12 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 membrane protein <400> 12 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 13 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 nucleocapsid protein <400> 13 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 14 <211> 28038 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p5 complete genome <400> 14 Ala Cys Thr Thr Ala Ala Ala Gly Ala Gly Ala Thr Thr Thr Thr Cys 1 5 10 15 Thr Ala Thr Cys Thr Ala Cys Gly Gly Ala Thr Ala Gly Thr Thr Ala 20 25 30 Gly Cys Thr Cys Thr Thr Thr Thr Thr Cys Thr Ala Gly Ala Cys Thr 35 40 45 Cys Thr Thr Gly Thr Cys Thr Ala Cys Thr Cys Ala Ala Thr Thr Cys 50 55 60 Ala Ala Cys Thr Ala Ala Ala Cys Gly Ala Ala Ala Thr Thr Thr Thr 65 70 75 80 Gly Thr Cys Cys Thr Thr Cys Cys Gly Gly Cys Cys Gly Cys Ala Thr 85 90 95 Gly Thr Cys Cys Ala Thr Gly Cys Thr Gly Cys Thr Gly Gly Ala Ala 100 105 110 Gly Cys Thr Gly Ala Cys Gly Thr Gly Gly Ala Ala Thr Thr Thr Cys 115 120 125 Ala Thr Thr Ala Gly Gly Thr Thr Thr Gly Cys Thr Thr Ala Ala Gly 130 135 140 Thr Ala Gly Cys Cys Ala Thr Cys Gly Cys Ala Ala Gly Thr Gly Cys 145 150 155 160 Thr Gly Thr Gly Cys Thr Gly Thr Cys Cys Thr Cys Thr Ala Gly Thr 165 170 175 Thr Cys Cys Thr Gly Gly Thr Thr Gly Gly Cys Gly Thr Thr Cys Cys 180 185 190 Gly Thr Cys Gly Cys Cys Thr Thr Cys Thr Ala Cys Ala Thr Ala Cys 195 200 205 Thr Ala Gly Ala Cys Ala Ala Ala Cys Ala Gly Cys Cys Thr Thr Cys 210 215 220 Cys Thr Cys Cys Gly Gly Thr Thr Cys Cys Gly Thr Cys Thr Gly Gly 225 230 235 240 Gly Gly Gly Thr Thr Gly Thr Gly Thr Gly Gly Ala Thr Ala Ala Cys 245 250 255 Thr Ala Gly Thr Thr Cys Cys Gly Thr Cys Thr Ala Gly Thr Thr Thr 260 265 270 Gly Ala Ala Ala Cys Cys Ala Gly Thr Ala Ala Cys Thr Gly Thr Cys 275 280 285 Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Ala Gly Cys Ala Ala Cys 290 295 300 Cys Ala Thr Gly Thr Thr Ala Cys Ala Thr Thr Gly Gly Cys Thr Thr 305 310 315 320 Thr Thr Gly Cys Cys Ala Ala Thr Gly Ala Thr Gly Cys Ala Gly Ala 325 330 335 Ala Ala Thr Thr Thr Cys Ala Gly Cys Thr Thr Thr Thr Gly Gly Cys 340 345 350 Thr Thr Thr Thr Gly Cys Ala Cys Thr Gly Cys Thr Ala Gly Thr Gly 355 360 365 Ala Ala Gly Cys Cys Gly Thr Cys Thr Cys Ala Thr Ala Cys Thr Ala 370 375 380 Thr Thr Cys Thr Gly Ala Gly Gly Cys Cys Gly Cys Cys Gly Cys Thr 385 390 395 400 Ala Gly Thr Gly Gly Ala Thr Thr Thr Ala Thr Gly Cys Ala Ala Thr 405 410 415 Gly Cys Cys Gly Thr Thr Thr Cys Gly Thr Gly Thr Cys Cys Thr Thr 420 425 430 Cys Gly Ala Thr Cys Thr Cys Gly Cys Thr Gly Ala Cys Ala Cys Thr 435 440 445 Gly Thr Thr Gly Ala Gly Gly Gly Ala Thr Thr Gly Cys Thr Thr Cys 450 455 460 Cys Cys Gly Ala Ala Gly Ala Cys Thr Ala Thr Gly Thr Cys Ala Thr 465 470 475 480 Gly Gly Thr Gly Gly Thr Gly Gly Thr Cys Gly Gly Cys Ala Cys Thr 485 490 495 Ala Cys Cys Ala Ala Gly Cys Thr Thr Ala Gly Thr Gly Cys Gly Thr 500 505 510 Ala Thr Gly Thr Gly Gly Ala Cys Ala Cys Thr Thr Thr Thr Gly Gly 515 520 525 Thr Ala Gly Cys Cys Gly Cys Cys Cys Cys Ala Ala Ala Ala Ala Cys 530 535 540 Ala Thr Thr Thr Gly Thr Gly Gly Thr Thr Gly Gly Cys Thr Gly Thr 545 550 555 560 Thr Ala Thr Thr Thr Thr Cys Thr Ala Ala Cys Thr Gly Thr Ala Ala 565 570 575 Thr Thr Ala Cys Thr Thr Cys Cys Thr Cys Gly Ala Ala Gly Ala Gly 580 585 590 Thr Thr Ala Gly Ala Gly Cys Thr Thr Ala Cys Thr Thr Thr Thr Gly 595 600 605 Gly Thr Cys Gly Thr Cys Gly Thr Gly Gly Thr Gly Gly Thr Ala Ala 610 615 620 Cys Ala Thr Cys Gly Thr Gly Cys Cys Ala Gly Thr Thr Gly Ala Cys 625 630 635 640 Cys Ala Ala Thr Ala Cys Ala Thr Gly Thr Gly Thr Gly Gly Cys Gly 645 650 655 Cys Thr Gly Ala Cys Gly Gly Thr Ala Ala Ala Cys Cys Thr Gly Thr 660 665 670 Thr Cys Thr Thr Cys Ala Gly Gly Ala Ala Thr Cys Cys Gly Ala Ala 675 680 685 Thr Gly Gly Gly Ala Gly Thr Ala Thr Ala Cys Ala Gly Ala Thr Thr 690 695 700 Thr Cys Thr Thr Thr Gly Cys Thr Gly Ala Cys Thr Cys Cys Gly Ala 705 710 715 720 Ala Gly Ala Cys Gly Gly Thr Cys Ala Ala Cys Thr Cys Ala Ala Cys 725 730 735 Ala Thr Thr Gly Cys Thr Gly Gly Thr Ala Thr Cys Ala Cys Thr Thr 740 745 750 Ala Thr Gly Thr Gly Ala Ala Gly Gly Cys Cys Thr Gly Gly Ala Thr 755 760 765 Thr Gly Thr Ala Gly Ala Gly Cys Gly Ala Thr Cys Gly Gly Ala Thr 770 775 780 Gly Thr Cys Thr Cys Thr Thr Ala Thr Gly Cys Gly Ala Gly Thr Cys 785 790 795 800 Ala Gly Ala Ala Thr Thr Thr Ala Ala Cys Ala Thr Cys Thr Ala Thr 805 810 815 Thr Ala Ala Gly Thr Cys Thr Ala Thr Thr Ala Cys Thr Thr Ala Cys 820 825 830 Thr Gly Thr Thr Cys Ala Ala Cys Cys Thr Ala Thr Gly Ala Gly Cys 835 840 845 Ala Thr Ala Cys Thr Thr Thr Thr Cys Cys Thr Gly Ala Thr Gly Gly 850 855 860 Thr Ala Cys Thr Gly Cys Cys Ala Thr Gly Ala Ala Gly Gly Thr Thr 865 870 875 880 Gly Cys Ala Cys Gly Thr Ala Cys Thr Cys Cys Ala Ala Ala Gly Ala 885 890 895 Thr Thr Ala Ala Gly Ala Ala Gly Ala Cys Thr Gly Thr Thr Gly Thr 900 905 910 Cys Thr Thr Gly Thr Cys Thr Gly Ala Gly Cys Cys Ala Cys Thr Thr 915 920 925 Gly Cys Thr Ala Cys Thr Ala Thr Cys Thr Ala Cys Ala Gly Gly Gly 930 935 940 Ala Ala Ala Thr Thr Gly Gly Thr Thr Cys Thr Cys Cys Thr Thr Thr 945 950 955 960 Thr Gly Thr Gly Gly Ala Thr Ala Ala Thr Gly Gly Gly Ala Gly Cys 965 970 975 Gly Ala Thr Gly Cys Thr Cys Gly Thr Thr Cys Thr Ala Thr Cys Ala 980 985 990 Thr Thr Ala Ala Gly Ala Gly Ala Cys Cys Ala Gly Thr Gly Thr Thr 995 1000 1005 Cys Cys Thr Cys Cys Ala Cys Gly Cys Thr Thr Thr Thr Gly Thr Thr 1010 1015 1020 Ala Ala Gly Thr Gly Thr Ala Ala Gly Thr Gly Thr Gly Gly Thr Ala 1025 1030 1035 1040 Gly Thr Thr Ala Thr Cys Ala Thr Thr Gly Gly Ala Cys Thr Gly Thr 1045 1050 1055 Thr Gly Gly Thr Gly Ala Thr Thr Gly Gly Ala Cys Thr Thr Cys Cys 1060 1065 1070 Thr Ala Thr Gly Thr Cys Thr Cys Cys Ala Cys Thr Thr Gly Cys Thr 1075 1080 1085 Gly Thr Gly Gly Cys Thr Thr Thr Ala Ala Gly Thr Gly Thr Ala Ala 1090 1095 1100 Gly Cys Cys Ala Gly Thr Cys Cys Thr Thr Gly Thr Gly Gly Cys Thr 1105 1110 1115 1120 Thr Cys Ala Thr Gly Cys Thr Cys Thr Gly Cys Thr Ala Cys Gly Cys 1125 1130 1135 Cys Thr Gly Gly Thr Thr Cys Thr Gly Thr Thr Gly Thr Gly Gly Thr 1140 1145 1150 Thr Ala Cys Gly Cys Gly Cys Gly Cys Thr Gly Gly Thr Gly Cys Thr 1155 1160 1165 Gly Gly Cys Ala Cys Thr Gly Gly Thr Gly Thr Thr Ala Ala Gly Thr 1170 1175 1180 Ala Thr Thr Ala Cys Ala Ala Cys Ala Ala Cys Ala Thr Gly Thr Thr 1185 1190 1195 1200 Cys Cys Thr Gly Cys Gly Cys Cys Ala Thr Gly Thr Gly Gly Cys Ala 1205 1210 1215 Gly Ala Cys Ala Thr Thr Gly Ala Thr Gly Gly Gly Thr Thr Gly Gly 1220 1225 1230 Cys Ala Thr Thr Cys Thr Gly Gly Cys Gly Ala Ala Thr Thr Cys Thr 1235 1240 1245 Cys Ala Ala Gly Gly Thr Gly Cys Ala Gly Thr Cys Cys Ala Ala Ala 1250 1255 1260 Gly Ala Cys Gly Ala Cys Cys Thr Cys Gly Cys Thr Thr Gly Cys Thr 1265 1270 1275 1280 Cys Thr Gly Gly Thr Ala Ala Ala Thr Thr Cys Cys Thr Thr Gly Ala 1285 1290 1295 Ala Cys Ala Cys Cys Ala Thr Gly Ala Gly Gly Ala Ala Gly Gly Thr 1300 1305 1310 Thr Thr Cys Ala Cys Ala Gly Ala Thr Cys Cys Thr Thr Gly Cys Thr 1315 1320 1325 Ala Cys Thr Thr Thr Thr Thr Gly Ala Ala Thr Gly Ala Cys Thr Cys 1330 1335 1340 Gly Ala Gly Cys Ala Thr Thr Gly Cys Thr Ala Cys Thr Ala Ala Gly 1345 1350 1355 1360 Cys Thr Cys Ala Ala Gly Thr Thr Thr Gly Ala Cys Ala Thr Cys Cys 1365 1370 1375 Thr Thr Ala Gly Thr Gly Gly Cys Ala Ala Gly Thr Thr Thr Thr Cys 1380 1385 1390 Thr Gly Ala Thr Gly Ala Ala Gly Thr Cys Ala Ala Ala Cys Ala Ala 1395 1400 1405 Gly Cys Thr Ala Thr Cys Thr Thr Thr Gly Cys Thr Gly Gly Thr Cys 1410 1415 1420 Ala Thr Gly Thr Thr Gly Thr Thr Gly Thr Thr Gly Gly Cys Ala Gly 1425 1430 1435 1440 Cys Gly Cys Gly Cys Thr Cys Gly Thr Thr Gly Ala Cys Ala Thr Thr 1445 1450 1455 Gly Thr Thr Gly Ala Cys Gly Ala Thr Gly Cys Ala Cys Thr Gly Gly 1460 1465 1470 Gly Ala Cys Ala Gly Cys Cys Thr Thr Gly Gly Thr Thr Thr Ala Thr 1475 1480 1485 Ala Cys Gly Thr Ala Ala Gly Cys Thr Thr Gly Gly Thr Gly Ala Cys 1490 1495 1500 Cys Thr Thr Gly Cys Ala Ala Gly Thr Gly Cys Ala Gly Cys Thr Thr 1505 1510 1515 1520 Gly Gly Gly Ala Gly Cys Ala Gly Cys Thr Thr Ala Ala Gly Gly Cys 1525 1530 1535 Thr Gly Thr Cys Gly Thr Thr Ala Gly Ala Gly Gly Cys Cys Thr Thr 1540 1545 1550 Ala Ala Cys Cys Thr Cys Cys Thr Gly Thr Cys Thr Gly Ala Thr Gly 1555 1560 1565 Ala Gly Gly Thr Cys Gly Thr Gly Cys Thr Cys Thr Thr Thr Gly Gly 1570 1575 1580 Cys Ala Ala Ala Ala Gly Ala Cys Thr Thr Ala Gly Cys Thr Gly Thr 1585 1590 1595 1600 Gly Cys Cys Ala Cys Thr Cys Thr Thr Ala Gly Thr Ala Thr Cys Gly 1605 1610 1615 Thr Thr Ala Ala Cys Gly Gly Thr Gly Thr Thr Thr Thr Thr Gly Ala 1620 1625 1630 Gly Thr Thr Cys Ala Thr Cys Gly Cys Cys Gly Ala Ala Gly Thr Gly 1635 1640 1645 Cys Cys Thr Gly Ala Gly Ala Ala Gly Thr Thr Gly Gly Cys Thr Gly 1650 1655 1660 Cys Gly Gly Cys Thr Gly Thr Thr Ala Cys Ala Gly Thr Thr Thr Thr 1665 1670 1675 1680 Thr Gly Thr Cys Ala Ala Cys Thr Thr Cys Thr Thr Gly Ala Ala Thr 1685 1690 1695 Gly Ala Gly Cys Thr Thr Thr Thr Thr Gly Ala Gly Thr Cys Thr Gly 1700 1705 1710 Cys Cys Thr Gly Thr Gly Ala Cys Thr Gly Cys Thr Thr Ala Ala Ala 1715 1720 1725 Gly Gly Thr Cys Gly Gly Ala Gly Gly Thr Ala Ala Ala Ala Cys Cys 1730 1735 1740 Thr Thr Thr Ala Ala Cys Ala Ala Gly Gly Thr Thr Gly Gly Cys Thr 1745 1750 1755 1760 Cys Thr Thr Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Gly Ala 1765 1770 1775 Cys Ala Ala Cys Gly Cys Ala Thr Thr Gly Gly Thr Thr Ala Ala Gly 1780 1785 1790 Cys Thr Thr Gly Thr Cys Ala Ala Gly Gly Cys Ala Ala Ala Ala Gly 1795 1800 1805 Thr Thr Cys Gly Cys Gly Gly Cys Cys Cys Ala Cys Gly Ala Cys Ala 1810 1815 1820 Gly Gly Cys Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Gly Ala Ala 1825 1830 1835 1840 Gly Thr Thr Cys Gly Thr Thr Ala Cys Ala Cys Ala Ala Gly Cys Cys 1845 1850 1855 Thr Thr Gly Thr Thr Ala Thr Thr Gly Gly Gly Ala Gly Thr Ala Cys 1860 1865 1870 Thr Ala Cys Cys Ala Ala Gly Gly Thr Gly Gly Thr Thr Thr Cys Cys 1875 1880 1885 Ala Ala Gly Cys Gly Cys Gly Thr Thr Gly Ala Ala Ala Ala Thr Gly 1890 1895 1900 Cys Cys Ala Ala Thr Gly Thr Gly Ala Ala Thr Cys Thr Cys Gly Thr 1905 1910 1915 1920 Cys Gly Thr Cys Gly Thr Thr Gly Ala Cys Gly Ala Gly Gly Ala Thr 1925 1930 1935 Gly Thr Gly Ala Cys Cys Cys Thr Cys Ala Ala Cys Ala Cys Cys Ala 1940 1945 1950 Cys Thr Gly Gly Thr Cys Gly Thr Ala Cys Ala Gly Thr Thr Gly Thr 1955 1960 1965 Thr Gly Thr Thr Gly Ala Cys Gly Gly Ala Cys Thr Thr Gly Cys Ala 1970 1975 1980 Thr Thr Cys Thr Thr Cys Gly Ala Gly Ala Gly Thr Gly Ala Cys Gly 1985 1990 1995 2000 Gly Gly Thr Thr Thr Thr Ala Cys Ala Gly Ala Cys Ala Thr Cys Thr 2005 2010 2015 Thr Gly Cys Thr Gly Ala Thr Gly Cys Thr Gly Ala Cys Gly Thr Thr 2020 2025 2030 Gly Thr Cys Ala Thr Thr Gly Ala Ala Cys Ala Thr Cys Cys Thr Gly 2035 2040 2045 Thr Thr Thr Ala Thr Ala Ala Gly Thr Cys Thr Gly Cys Thr Thr Gly 2050 2055 2060 Thr Gly Ala Gly Cys Thr Cys Ala Ala Gly Cys Cys Ala Gly Thr Thr 2065 2070 2075 2080 Thr Thr Thr Gly Ala Gly Thr Gly Thr Gly Ala Cys Cys Cys Ala Ala 2085 2090 2095 Thr Ala Cys Cys Thr Gly Ala Thr Thr Thr Thr Cys Cys Thr Ala Thr 2100 2105 2110 Gly Cys Cys Thr Gly Thr Gly Gly Cys Cys Gly Cys Thr Ala Gly Thr 2115 2120 2125 Gly Thr Thr Gly Cys Ala Gly Ala Gly Cys Thr Thr Thr Gly Thr Gly 2130 2135 2140 Thr Gly Cys Ala Ala Ala Cys Thr Gly Ala Thr Cys Thr Gly Thr Thr 2145 2150 2155 2160 Gly Cys Thr Thr Ala Ala Ala Ala Ala Thr Thr Ala Cys Ala Ala Cys 2165 2170 2175 Ala Cys Thr Cys Cys Thr Thr Ala Thr Ala Ala Ala Ala Cys Thr Thr 2180 2185 2190 Ala Cys Ala Gly Cys Thr Gly Cys Gly Thr Thr Gly Thr Gly Ala Gly 2195 2200 2205 Ala Gly Gly Thr Gly Ala Thr Ala Ala Gly Thr Gly Thr Thr Gly Cys 2210 2215 2220 Ala Thr Cys Ala Cys Thr Thr Gly Cys Ala Cys Cys Thr Thr Ala Cys 2225 2230 2235 2240 Ala Thr Ala Thr Cys Ala Cys Ala Gly Cys Ala Cys Cys Ala Ala Gly 2245 2250 2255 Thr Thr Ala Thr Ala Thr Gly Gly Ala Gly Gly Ala Thr Gly Cys Thr 2260 2265 2270 Gly Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Ala Gly Ala Cys Cys 2275 2280 2285 Thr Cys Thr Gly Thr Ala Cys Cys Ala Ala Gly Ala Ala Cys Ala Thr 2290 2295 2300 Thr Gly Gly Thr Ala Cys Thr Gly Cys Thr Gly Gly Thr Thr Thr Thr 2305 2310 2315 2320 Cys Ala Thr Gly Ala Gly Thr Thr Thr Thr Ala Cys Ala Thr Thr Ala 2325 2330 2335 Cys Gly Gly Cys Cys Cys Ala Thr Gly Ala Ala Cys Ala Ala Cys Ala 2340 2345 2350 Gly Gly Ala Thr Cys Thr Gly Cys Ala Ala Gly Gly Gly Thr Thr Cys 2355 2360 2365 Gly Thr Ala Ala Cys Cys Ala Cys Thr Thr Gly Thr Thr Gly Cys Ala 2370 2375 2380 Cys Gly Ala Thr Gly Thr Cys Ala Gly Gly Thr Thr Thr Thr Gly Ala 2385 2390 2395 2400 Gly Thr Gly Thr Thr Thr Thr Ala Thr Gly Cys Cys Thr Ala Thr Ala 2405 2410 2415 Ala Thr Cys Cys Cys Ala Cys Ala Gly Thr Gly Thr Cys Cys Ala Gly 2420 2425 2430 Cys Ala Gly Thr Gly Cys Thr Thr Gly Ala Ala Gly Ala Gly Ala Thr 2435 2440 2445 Thr Gly Ala Thr Gly Gly Thr Gly Gly Thr Ala Gly Cys Ala Thr Cys 2450 2455 2460 Thr Gly Gly Cys Gly Gly Thr Cys Thr Thr Thr Thr Ala Thr Cys Ala 2465 2470 2475 2480 Cys Thr Gly Gly Thr Cys Thr Thr Ala Ala Thr Ala Cys Ala Ala Thr 2485 2490 2495 Gly Thr Gly Gly Gly Ala Thr Thr Thr Thr Thr Gly Cys Ala Ala Gly 2500 2505 2510 Cys Ala Thr Cys Thr Thr Ala Ala Ala Gly Thr Cys Ala Gly Cys Thr 2515 2520 2525 Thr Thr Gly Gly Ala Cys Thr Ala Gly Ala Thr Gly Gly Cys Ala Thr 2530 2535 2540 Thr Gly Thr Thr Gly Thr Cys Ala Cys Thr Gly Thr Ala Gly Cys Ala 2545 2550 2555 2560 Cys Gly Cys Ala Ala Ala Thr Thr Thr Ala Ala Ala Cys Gly Ala Cys 2565 2570 2575 Thr Thr Gly Gly Thr Gly Cys Thr Cys Thr Cys Thr Thr Gly Gly Cys 2580 2585 2590 Ala Gly Ala Ala Ala Thr Gly Thr Ala Thr Ala Ala Cys Ala Cys Thr 2595 2600 2605 Thr Ala Cys Cys Thr Thr Thr Cys Ala Ala Cys Thr Gly Thr Gly Gly 2610 2615 2620 Thr Gly Gly Ala Ala Ala Ala Cys Thr Thr Gly Gly Thr Ala Cys Thr 2625 2630 2635 2640 Gly Gly Cys Cys Gly Gly Thr Gly Thr Thr Ala Gly Cys Thr Thr Cys 2645 2650 2655 Ala Ala Gly Thr Ala Thr Thr Ala Thr Gly Cys Cys Ala Cys Cys Ala 2660 2665 2670 Gly Thr Gly Thr Cys Cys Cys Ala Ala Ala Ala Ala Thr Thr Gly Thr 2675 2680 2685 Thr Thr Thr Gly Gly Gly Cys Thr Gly Thr Thr Gly Thr Thr Thr Thr 2690 2695 2700 Cys Ala Cys Ala Gly Thr Gly Thr Thr Ala Ala Ala Ala Gly Thr Gly 2705 2710 2715 2720 Thr Thr Cys Thr Thr Gly Cys Ala Ala Gly Thr Gly Cys Cys Thr Thr 2725 2730 2735 Cys Cys Ala Gly Ala Thr Thr Cys Cys Thr Gly Thr Cys Cys Ala Gly 2740 2745 2750 Gly Cys Ala Gly Gly Cys Ala Thr Thr Gly Ala Gly Ala Ala Gly Thr 2755 2760 2765 Thr Thr Ala Ala Ala Gly Thr Cys 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26865 26870 26875 26880 Ala Thr Ala Ala Cys Cys Ala Gly Thr Cys Cys Cys Gly Cys Gly Gly 26885 26890 26895 Thr Ala Ala Thr Thr Cys Ala Cys Ala Gly Ala Ala Thr Cys Gly Thr 26900 26905 26910 Gly Gly Ala Ala Ala Thr Ala Ala Cys Cys Ala Gly Gly Gly Thr Cys 26915 26920 26925 Gly Thr Gly Gly Ala Gly Cys Thr Thr Cys Thr Cys Ala Gly Ala Ala 26930 26935 26940 Cys Ala Gly Ala Gly Gly Ala Gly Gly Cys Ala Ala Thr Ala Ala Thr 26945 26950 26955 26960 Ala Ala Thr Ala Ala Cys Ala Ala Thr Ala Ala Cys Ala Ala Gly Thr 26965 26970 26975 Cys Thr Cys Gly Thr Ala Ala Cys Cys Ala Gly Thr Cys Cys Ala Ala 26980 26985 26990 Gly Ala Ala Cys Ala Gly Ala Ala Ala Cys Cys Ala Gly Thr Cys Ala 26995 27000 27005 Ala Ala Thr Gly Ala Cys Cys Gly Thr Gly Gly Thr Gly Gly Thr Gly 27010 27015 27020 Thr Ala Ala Cys Ala Thr Cys Ala Cys Gly Cys Gly Ala Thr Gly Ala 27025 27030 27035 27040 Thr Cys Thr Gly Gly Thr Gly Gly Cys Thr Gly Cys Thr Gly Thr Cys 27045 27050 27055 Ala Ala Gly Gly Ala Thr Gly Cys Cys Cys Thr Thr Ala Ala Ala 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27250 27255 27260 Gly Ala Cys Cys Cys Ala Gly Gly Gly Gly Ala Gly Gly Cys Thr Thr 27265 27270 27275 27280 Cys Ala Ala Ala Ala Ala Thr Thr Thr Thr Gly Gly Ala Gly Ala Thr 27285 27290 27295 Gly Cys Gly Gly Ala Ala Thr Thr Thr Gly Thr Cys Gly Ala Ala Ala 27300 27305 27310 Ala Ala Gly Gly Thr Gly Thr Thr Gly Ala Thr Gly Cys Cys Thr Cys 27315 27320 27325 Ala Gly Gly Cys Thr Ala Thr Gly Cys Thr Cys Ala Gly Ala Thr Cys 27330 27335 27340 Gly Cys Cys Ala Gly Thr Thr Thr Ala Gly Cys Ala Cys Cys Ala Ala 27345 27350 27355 27360 Ala Thr Gly Thr Thr Gly Cys Ala Gly Cys Ala Thr Thr Gly Cys Thr 27365 27370 27375 Cys Thr Thr Thr Gly Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Gly 27380 27385 27390 Gly Cys Thr Gly Thr Thr Cys Gly Thr Gly Ala Gly Cys Thr Ala Gly 27395 27400 27405 Cys Gly Gly Ala Cys Thr Cys Thr Thr Ala Cys Gly Ala Gly Ala Thr 27410 27415 27420 Thr Ala Cys Ala Thr Ala Thr Ala Ala Thr Thr Ala Thr Ala Ala Ala 27425 27430 27435 27440 Ala Thr Gly Ala Cys Thr Gly Thr Gly Cys Cys Ala Ala Ala Gly 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27635 27640 27645 Ala Thr Gly Gly Thr Gly Gly Thr Gly Ala Cys Ala Cys Gly Gly Cys 27650 27655 27660 Cys Gly Thr Thr Gly Ala Ala Ala Thr Thr Ala Thr Cys Ala Ala Cys 27665 27670 27675 27680 Gly Ala Gly Ala Thr Cys Thr Thr Cys Gly Ala Cys Ala Cys Ala Gly 27685 27690 27695 Gly Ala Ala Ala Thr Thr Ala Ala Ala Cys Ala Ala Thr Gly Thr Thr 27700 27705 27710 Thr Gly Ala Cys Thr Gly Gly Cys Thr Thr Ala Thr Cys Cys Thr Gly 27715 27720 27725 Gly Cys Thr Ala Thr Gly Thr Cys Cys Cys Ala Gly Gly Gly Thr Ala 27730 27735 27740 Gly Thr Gly Cys Cys Ala Thr Thr Ala Cys Ala Cys Thr Gly Thr Thr 27745 27750 27755 27760 Ala Thr Thr Ala Cys Thr Gly Ala Gly Thr Gly Thr Thr Thr Thr Thr 27765 27770 27775 Cys Thr Ala Gly Cys Gly Ala Cys Thr Thr Gly Gly Cys Thr Gly Cys 27780 27785 27790 Thr Gly Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Thr Thr Gly Cys 27795 27800 27805 Cys Cys Thr Cys Thr Ala Ala Cys Thr Ala Gly Cys Gly Gly Thr Cys 27810 27815 27820 Thr Thr Gly Gly Thr Cys Thr Thr Gly Cys Ala Cys Ala Cys Ala Ala 27825 27830 27835 27840 Cys Gly Gly Thr Ala Ala Gly Cys Cys Ala Gly Thr Gly Gly Thr Ala 27845 27850 27855 Ala Thr Gly Thr Cys Ala Gly Thr Gly Cys Ala Ala Gly Ala Ala Gly 27860 27865 27870 Gly Ala Thr Ala Thr Thr Ala Cys Cys Ala Thr Ala Gly Cys Ala Cys 27875 27880 27885 Thr Gly Thr Cys Ala Thr Gly Ala Gly Gly Gly Gly Ala Ala Cys Gly 27890 27895 27900 Cys Ala Gly Thr Ala Cys Cys Thr Thr Thr Thr Cys Ala Thr Cys Thr 27905 27910 27915 27920 Ala Ala Ala Cys Cys Thr Thr Thr Gly Cys Ala Cys Gly Ala Gly Thr 27925 27930 27935 Ala Ala Thr Cys Ala Ala Ala Gly Ala Thr Cys Cys Gly Cys Thr Thr 27940 27945 27950 Gly Ala Cys Gly Ala Gly Cys Cys Thr Ala Thr Ala Thr Gly Gly Ala 27955 27960 27965 Ala Gly Ala Gly Cys Gly Thr Gly Cys Cys Ala Gly Gly Thr Ala Thr 27970 27975 27980 Thr Thr Gly Ala Cys Thr Cys Ala Ala Gly Gly Ala Cys Thr Gly Thr 27985 27990 27995 28000 Thr Ala Gly Thr Ala Ala Cys Thr Gly Ala Ala Gly Ala Cys Cys Thr 28005 28010 28015 Gly Ala Cys Gly Gly Thr Gly Thr Thr Gly Ala Thr Ala Thr Gly Gly 28020 28025 28030 Ala Thr Ala Cys Ala Cys 28035 <210> 15 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-p10 spike protein <400> 15 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Thr 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Gln Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 16 <211> 6781 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 ORF1a/1b <400> 16 Met Ala Ser Asn His Val Thr Leu Ala Phe Ala Asn Asp Ala Glu Ile 1 5 10 15 Ser Ala Phe Gly Phe Cys Thr Ala Ser Glu Ala Val Ser Tyr Tyr Ser 20 25 30 Glu Ala Ala Ala Ser Gly Phe Met Gln Cys Arg Phe Val Ser Phe Asp 35 40 45 Leu Ala Asp Thr Val Glu Gly Leu Leu Pro Glu Asp Tyr Val Met Val 50 55 60 Val Val Gly Thr Thr Lys Leu Ser Ala Tyr Val Asp Thr Phe Gly Ser 65 70 75 80 Arg Pro Lys Asn Ile Cys Gly Trp Leu Leu Phe Ser Asn Cys Asn Tyr 85 90 95 Phe Leu Glu Glu Leu Glu Leu Thr Phe Gly Arg Arg Gly Gly Asn Ile 100 105 110 Val Pro Val Asp Gln Tyr Met Cys Gly Ala Asp Gly Lys Pro Val Leu 115 120 125 Gln Glu Ser Glu Trp Glu Tyr Thr Asp Phe Phe Ala Asp Ser Glu Asp 130 135 140 Gly Gln Leu Asn Ile Ala Gly Ile Thr Tyr Val Lys Ala Trp Ile Val 145 150 155 160 Glu Arg Ser Asp Val Ser Tyr Ala Ser Gln Asn Leu Thr Ser Ile Lys 165 170 175 Ser Ile Thr Tyr Cys Ser Thr Tyr Glu His Thr Phe Pro Asp Gly Thr 180 185 190 Ala Met Lys Val Ala Arg Thr Pro Lys Ile Lys Lys Thr Val Val Leu 195 200 205 Ser Glu Pro Leu Ala Thr Ile Tyr Arg Glu Ile Gly Ser Pro Phe Val 210 215 220 Asp Asn Gly Ser Asp Ala Arg Ser Ile Ile Lys Arg Pro Val Phe Leu 225 230 235 240 His Ala Phe Val Lys Cys Lys Cys Gly Ser Tyr His Trp Thr Val Gly 245 250 255 Asp Trp Thr Ser Tyr Val Ser Thr Cys Cys Gly Phe Lys Cys Lys Pro 260 265 270 Val Leu Val Ala Ser Cys Ser Ala Thr Pro Gly Ser Val Val Val Thr 275 280 285 Arg Ala Gly Ala Gly Thr Gly Val Lys Tyr Tyr Asn Asn Met Phe Leu 290 295 300 Arg His Val Ala Asp Ile Asp Gly Leu Ala Phe Trp Arg Ile Leu Lys 305 310 315 320 Val Gln Ser Lys Asp Asp Leu Ala Cys Ser Gly Lys Phe Leu Glu His 325 330 335 His Glu Glu Gly Phe Thr Asp Pro Cys Tyr Phe Leu Asn Asp Ser Ser 340 345 350 Ile Ala Thr Lys Leu Lys Phe Asp Ile Leu Ser Gly Lys Phe Ser Asp 355 360 365 Glu Val Lys Gln Ala Ile Phe Ala Gly His Val Val Val Gly Ser Ala 370 375 380 Leu Val Asp Ile Val Asp Asp Ala Leu Gly Gln Pro Trp Phe Ile Arg 385 390 395 400 Lys Leu Gly Asp Leu Ala Ser Ala Ala Trp Glu Gln Leu Lys Ala Val 405 410 415 Val Arg Gly Leu Asn Leu Leu Ser Asp Glu Val Val Leu Phe Gly Lys 420 425 430 Arg Leu Ser Cys Ala Thr Leu Ser Ile Val Asn Gly Val Phe Glu Phe 435 440 445 Ile Ala Glu Val Pro Glu Lys Leu Ala Ala Ala Val Thr Val Phe Val 450 455 460 Asn Phe Leu Asn Glu Leu Phe Glu Ser Ala Cys Asp Cys Leu Lys Val 465 470 475 480 Gly Gly Lys Thr Phe Asn Lys Val Gly Ser Tyr Val Leu Phe Asp Asn 485 490 495 Ala Leu Val Lys Leu Val Lys Ala Lys Val Arg Gly Pro Arg Gln Ala 500 505 510 Gly Val Cys Glu Val Arg Tyr Thr Ser Leu Val Ile Gly Ser Thr Thr 515 520 525 Lys Val Val Ser Lys Arg Val Glu Asn Ala Asn Val Asn Leu Val Val 530 535 540 Val Asp Glu Asp Val Thr Leu Asn Thr Thr Gly Arg Thr Val Val Val 545 550 555 560 Asp Gly Leu Ala Phe Phe Glu Ser Asp Gly Phe Tyr Arg His Leu Ala 565 570 575 Asp Ala Asp Val Val Ile Glu His Pro Val Tyr Lys Ser Ala Cys Glu 580 585 590 Leu Lys Pro Val Phe Glu Cys Asp Pro Ile Pro Asp Phe Pro Met Pro 595 600 605 Val Ala Ala Ser Val Ala Glu Leu Cys Val Gln Thr Asp Leu Leu Leu 610 615 620 Lys Asn Tyr Asn Thr Pro Tyr Lys Thr Tyr Ser Cys Val Val Arg Gly 625 630 635 640 Asp Lys Cys Cys Ile Thr Cys Thr Leu His Ile Thr Ala Pro Ser Tyr 645 650 655 Met Glu Asp Ala Ala Asn Phe Val Asp Leu Cys Thr Lys Asn Ile Gly 660 665 670 Thr Ala Gly Phe His Glu Phe Tyr Ile Thr Ala His Glu Gln Gln Asp 675 680 685 Leu Gln Gly Phe Val Thr Thr Cys Cys Thr Met Ser Gly Phe Glu Cys 690 695 700 Phe Met Pro Ile Ile Pro Gln Cys Pro Ala Val Leu Glu Glu Ile Asp 705 710 715 720 Gly Gly Ser Ile Trp Arg Ser Phe Ile Thr Gly Leu Asn Thr Met Trp 725 730 735 Asp Phe Cys Lys His Leu Lys Val Ser Phe Gly Leu Asp Gly Ile Val 740 745 750 Val Thr Val Ala Arg Lys Phe Lys Arg Leu Gly Ala Leu Leu Ala Glu 755 760 765 Met Tyr Asn Thr Tyr Leu Ser Thr Val Val Glu Asn Leu Val Leu Ala 770 775 780 Gly Val Ser Phe Lys Tyr Tyr Ala Thr Ser Val Pro Lys Ile Val Leu 785 790 795 800 Gly Cys Cys Phe His Ser Val Lys Ser Val Leu Ala Ser Ala Phe Gln 805 810 815 Ile Pro Val Gln Ala Gly Ile Glu Lys Phe Lys Val Phe Leu Asn Cys 820 825 830 Val His Pro Val Val Pro Arg Val Ile Glu Thr Ser Phe Val Glu Leu 835 840 845 Glu Glu Thr Thr Phe Lys Pro Pro Ala Leu Asn Gly Ser Ile Ala Ile 850 855 860 Val Asp Gly Phe Ala Phe Tyr Tyr Asp Gly Thr Leu Tyr Tyr Pro Thr 865 870 875 880 Asp Gly Asn Ser Val Val Pro Ile Cys Phe Lys Lys Lys Gly Gly Gly 885 890 895 Asp Val Lys Phe Ser Asp Glu Val Ser Val Arg Thr Ile Asp Pro Val 900 905 910 Tyr Lys Val Ser Leu Glu Phe Glu Phe Glu Ser Glu Thr Ile Met Ala 915 920 925 Val Leu Asn Lys Ala Val Gly Asn Arg Ile Lys Val Thr Gly Gly Trp 930 935 940 Asp Asp Val Val Glu Tyr Ile Asn Val Ala Ile Glu Val Leu Lys Asp 945 950 955 960 His Ile Asp Val Pro Lys Tyr Tyr Ile Tyr Asp Glu Glu Gly Gly Thr 965 970 975 Asp Pro Asn Leu Pro Val Met Val Ser Gln Trp Pro Leu Asn Asp Asp 980 985 990 Thr Ile Ser Gln Asp Leu Leu Asp Val Glu Val Val Thr Asp Ala Pro 995 1000 1005 Ile Asp Phe Glu Gly Asp Glu Val Asp Ser Ser Asp Pro Asp Lys Val 1010 1015 1020 Ala Asp Val Ala Asn Ser Glu Pro Glu Asp Asp Gly Pro Asn Val Ala 1025 1030 1035 1040 Pro Glu Thr Asn Val Glu Ser Glu Val Glu Glu Val Ala Ala Thr Leu 1045 1050 1055 Ser Phe Ile Lys Asp Thr Pro Ser Thr Val Thr Lys Asp Pro Phe Ala 1060 1065 1070 Phe Asp Phe Ala Ser Tyr Gly Gly Leu Lys Val Leu Arg Gln Ser His 1075 1080 1085 Asn Asn Cys Trp Val Thr Ser Thr Leu Val Gln Leu Gln Leu Leu Gly 1090 1095 1100 Ile Val Asp Asp Pro Ala Met Glu Leu Phe Ser Ala Gly Arg Val Gly 1105 1110 1115 1120 Pro Met Val Arg Lys Cys Tyr Glu Ser Gln Lys Ala Ile Leu Gly Ser 1125 1130 1135 Leu Gly Asp Val Ser Ala Cys Leu Glu Ser Leu Thr Lys Asp Leu His 1140 1145 1150 Thr Leu Lys Ile Thr Cys Ser Val Val Cys Gly Cys Gly Thr Gly Glu 1155 1160 1165 Arg Ile Tyr Glu Gly Cys Ala Phe Arg Met Thr Pro Thr Leu Glu Pro 1170 1175 1180 Phe Pro Tyr Gly Ala Cys Ala Gln Cys Ala Gln Val Leu Met His Thr 1185 1190 1195 1200 Phe Lys Ser Ile Val Gly Thr Gly Ile Phe Cys Arg Asp Thr Thr Ala 1205 1210 1215 Leu Ser Leu Asp Ser Leu Val Val Lys Pro Leu Cys Ala Ala Ala Phe 1220 1225 1230 Ile Gly Lys Asp Ser Gly His Tyr Val Thr Asn Phe Tyr Asp Ala Ala 1235 1240 1245 Met Ala Ile Asp Gly Tyr Gly Arg His Gln Ile Lys Tyr Asp Thr Leu 1250 1255 1260 Asn Thr Ile Cys Val Lys Asp Val Asn Trp Thr Ala Pro Phe Val Pro 1265 1270 1275 1280 Asp Val Glu Pro Val Leu Glu Pro Val Val Lys Pro Phe Tyr Ser Tyr 1285 1290 1295 Lys Asn Val Asp Phe Tyr Gln Gly Asp Phe Ser Asp Leu Val Lys Leu 1300 1305 1310 Pro Cys Asp Phe Val Val Asn Ala Ala Asn Glu Asn Leu Ser His Gly 1315 1320 1325 Gly Gly Ile Ala Lys Ala Ile Asp Val Tyr Thr Lys Gly Met Leu Gln 1330 1335 1340 Lys Cys Ser Asn Asp Tyr Ile Lys Ala His Gly Pro Ile Lys Val Gly 1345 1350 1355 1360 Arg Gly Val Met Leu Glu Ala Leu Gly Leu Lys Val Phe Asn Val Val 1365 1370 1375 Gly Pro Arg Lys Gly Lys His Ala Pro Glu Leu Leu Val Lys Ala Tyr 1380 1385 1390 Lys Ser Val Phe Ala Asn Ser Gly Val Ala Leu Thr Pro Leu Ile Ser 1395 1400 1405 Val Gly Ile Phe Ser Val Pro Leu Glu Glu Ser Leu Ser Ala Phe Leu 1410 1415 1420 Ala Cys Val Gly Asp Arg His Cys Lys Cys Phe Cys Tyr Ser Asp Lys 1425 1430 1435 1440 Glu Arg Glu Ala Ile Ile Asn Tyr Met Asp Gly Leu Val Asp Ala Ile 1445 1450 1455 Phe Lys Asp Ala Leu Val Asp Thr Thr Pro Val Gln Glu Asp Val Gln 1460 1465 1470 Gln Val Ser Gln Lys Pro Val Leu Pro Asn Phe Glu Pro Phe Arg Ile 1475 1480 1485 Glu Gly Ala His Ala Phe Tyr Glu Cys Asn Pro Glu Gly Leu Met Ser 1490 1495 1500 Leu Gly Ala Asp Lys Leu Val Leu Phe Thr Asn Ser Asn Leu Asp Phe 1505 1510 1515 1520 Cys Ser Val Gly Lys Cys Leu Asn Asn Val Thr Gly Gly Ala Leu Leu 1525 1530 1535 Glu Ala Ile Asn Val Phe Lys Lys Ser Asn Lys Thr Val Pro Ala Gly 1540 1545 1550 Asn Cys Val Thr Phe Glu Cys Ala Asp Met Ile Ser Ile Thr Met Val 1555 1560 1565 Val Leu Pro Ser Asp Gly Asp Ala Asn Tyr Asp Lys Asn Tyr Ala Arg 1570 1575 1580 Ala Val Val Lys Val Ser Lys Leu Lys Gly Lys Leu Leu Leu Ala Val 1585 1590 1595 1600 Gly Asp Ala Met Leu Tyr Ser Lys Leu Ser His Leu Ser Val Leu Gly 1605 1610 1615 Phe Val Ser Thr Pro Asp Asp Val Glu Arg Phe Tyr Ala Asn Lys Ser 1620 1625 1630 Val Val Ile Lys Val Thr Glu Asp Thr Arg Ser Val Lys Thr Val Lys 1635 1640 1645 Val Glu Ser Thr Val Thr Tyr Gly Gln Gln Ile Gly Pro Cys Leu Val 1650 1655 1660 Asn Asp Thr Val Val Thr Asp Asn Lys Pro Val Val Ala Asp Val Val 1665 1670 1675 1680 Ala Lys Val Val Pro Ser Ala Asn Trp Asp Ser His Tyr Gly Phe Asp 1685 1690 1695 Lys Ala Gly Glu Phe His Met Leu Asp His Thr Gly Phe Ala Phe Pro 1700 1705 1710 Ser Glu Val Val Asn Gly Arg Arg Val Leu Lys Thr Thr Asp Asn Asn 1715 1720 1725 Cys Trp Val Asn Val Thr Cys Leu Gln Leu Gln Phe Ala Arg Phe Arg 1730 1735 1740 Phe Lys Ser Ala Gly Leu Gln Ala Met Trp Glu Ser Tyr Cys Thr Gly 1745 1750 1755 1760 Asp Val Ala Met Phe Val His Trp Leu Tyr Trp Leu Thr Gly Val Asp 1765 1770 1775 Lys Gly Gln Pro Ser Asp Ser Glu Asn Ala Leu Asn Met Leu Ser Lys 1780 1785 1790 Tyr Ile Val Pro Ala Gly Ser Val Thr Ile Glu Arg Val Thr His Asp 1795 1800 1805 Gly Cys Cys Cys Ser Lys Arg Val Val Thr Ala Pro Val Val Asn Ala 1810 1815 1820 Ser Val Leu Lys Leu Gly Val Glu Asp Gly Leu Cys Pro His Gly Leu 1825 1830 1835 1840 Asn Tyr Ile Asp Lys Val Val Val Val Lys Gly Thr Thr Ile Val Val 1845 1850 1855 Asn Val Gly Lys Pro Val Val Ala Pro Ser His Leu Phe Leu Lys Gly 1860 1865 1870 Val Ser Tyr Thr Thr Phe Leu Asp Asn Gly Asn Gly Val Ala Gly His 1875 1880 1885 Tyr Thr Val Phe Asp His Asp Thr Gly Met Val His Asp Gly Asp Val 1890 1895 1900 Phe Val Pro Gly Asp Leu Asn Val Ser Pro Val Thr Asn Val Val Val 1905 1910 1915 1920 Ser Glu Gln Thr Ala Val Val Ile Lys Asp Pro Val Lys Lys Val Glu 1925 1930 1935 Leu Asp Ala Thr Lys Leu Leu Asp Thr Met Asn Tyr Ala Ser Glu Arg 1940 1945 1950 Phe Phe Ser Phe Gly Asp Phe Met Ser Arg Asn Leu Ile Thr Val Phe 1955 1960 1965 Leu Tyr Ile Leu Ser Ile Leu Gly Leu Cys Phe Arg Ala Phe Arg Lys 1970 1975 1980 Arg Asp Val Lys Val Leu Ala Gly Val Pro Gln Arg Thr Gly Ile Ile 1985 1990 1995 2000 Leu Arg Lys Ser Val Arg Tyr Asn Ala Lys Ala Leu Gly Val Phe Phe 2005 2010 2015 Lys Leu Lys Leu Tyr Trp Phe Lys Val Leu Gly Lys Phe Ser Leu Gly 2020 2025 2030 Ile Tyr Ala Leu Tyr Ala Leu Leu Phe Met Thr Ile Arg Phe Thr Pro 2035 2040 2045 Ile Gly Gly Pro Val Cys Asp Asp Val Val Ala Gly Tyr Ala Asn Ser 2050 2055 2060 Ser Phe Asp Lys Asn Glu Tyr Cys Asn Ser Val Ile Cys Lys Val Cys 2065 2070 2075 2080 Leu Tyr Gly Tyr Gln Glu Leu Ser Asp Phe Ser His Thr Gln Val Val 2085 2090 2095 Trp Gln His Leu Arg Asp Pro Leu Ile Gly Asn Val Met Pro Phe Phe 2100 2105 2110 Tyr Leu Ala Phe Leu Ala Ile Phe Gly Gly Val Tyr Val Lys Ala Ile 2115 2120 2125 Thr Leu Tyr Phe Ile Phe Gln Tyr Leu Asn Ile Leu Gly Val Phe Leu 2130 2135 2140 Gly Leu Gln Gln Ser Ile Trp Phe Leu Gln Leu Val Pro Phe Asp Val 2145 2150 2155 2160 Phe Gly Asp Glu Ile Val Val Phe Phe Ile Val Thr Arg Val Leu Met 2165 2170 2175 Phe Leu Lys His Val Phe Leu Gly Cys Asp Lys Ala Ser Cys Val Ala 2180 2185 2190 Cys Ser Lys Ser Ala Arg Leu Lys Arg Val Pro Val Gln Thr Ile Phe 2195 2200 2205 Gln Gly Thr Ser Lys Ser Phe Tyr Val His Ala Asn Gly Gly Ser Lys 2210 2215 2220 Phe Cys Lys Lys His Asn Phe Phe Cys Leu Asn Cys Asp Ser Tyr Gly 2225 2230 2235 2240 Pro Gly Cys Thr Phe Ile Asn Asp Val Ile Ala Thr Glu Val Gly Asn 2245 2250 2255 Val Val Lys Leu Asn Val Gln Pro Thr Gly Pro Ala Thr Ile Leu Ile 2260 2265 2270 Asp Lys Val Glu Phe Ser Asn Gly Phe Tyr Tyr Leu Tyr Ser Gly Asp 2275 2280 2285 Thr Phe Trp Lys Tyr Asn Phe Asp Ile Thr Asp Asn Lys Tyr Thr Cys 2290 2295 2300 Lys Glu Ser Leu Lys Asn Cys Ser Ile Ile Thr Asp Phe Ile Val Phe 2305 2310 2315 2320 Asn Asn Asn Gly Ser Asn Val Asn Gln Val Lys Asn Ala Cys Val Tyr 2325 2330 2335 Phe Ser Gln Met Leu Cys Lys Pro Val Lys Leu Val Asp Ser Ala Leu 2340 2345 2350 Leu Ala Ser Leu Ser Val Asp Phe Gly Ala Ser Leu His Ser Ala Phe 2355 2360 2365 Val Ser Val Leu Ser Asn Ser Phe Gly Lys Asp Leu Ser Ser Cys Asn 2370 2375 2380 Asp Met Gln Asp Cys Lys Ser Thr Leu Gly Phe Asp Asp Val Pro Leu 2385 2390 2395 2400 Asp Thr Phe Asn Ala Ala Val Ala Glu Ala His Arg Tyr Asp Val Leu 2405 2410 2415 Leu Thr Asp Met Ser Phe Asn Asn Phe Thr Thr Ser Tyr Ala Lys Pro 2420 2425 2430 Glu Glu Lys Leu Pro Val His Asp Ile Ala Thr Cys Met Arg Val Gly 2435 2440 2445 Ala Lys Ile Val Asn His Asn Val Leu Val Lys Asp Ser Ile Pro Val 2450 2455 2460 Val Trp Leu Val Arg Asp Phe Ile Val Leu Ser Glu Glu Thr Arg Lys 2465 2470 2475 2480 Tyr Ile Ile Arg Thr Thr Lys Val Lys Gly Ile Thr Phe Met Leu Thr 2485 2490 2495 Phe Asn Asp Cys Arg Met His Thr Thr Ile Pro Thr Val Cys Ile Ala 2500 2505 2510 Asn Lys Lys Gly Ala Gly Leu Pro Ser Phe Ser Lys Val Lys Lys Phe 2515 2520 2525 Phe Trp Phe Leu Cys Leu Phe Ile Val Ala Val Phe Phe Ala Leu Ser 2530 2535 2540 Phe Phe Asp Phe Ser Thr Gln Val Ser Ser Asp Ser Asp Tyr Asp Phe 2545 2550 2555 2560 Lys Tyr Ile Glu Ser Gly Gln Leu Lys Thr Phe Asp Asn Pro Leu Ser 2565 2570 2575 Cys Val His Asn Val Phe Ser Asn Phe Asp Gln Trp His Asp Ala Lys 2580 2585 2590 Phe Gly Phe Thr Pro Val Asn Asn Pro Ser Cys Pro Ile Val Val Gly 2595 2600 2605 Val Ser Asp Glu Ala Arg Thr Val Pro Gly Ile Pro Ala Gly Val Tyr 2610 2615 2620 Leu Ala Gly Lys Thr Leu Val Phe Ala Ile Asn Thr Ile Phe Gly Thr 2625 2630 2635 2640 Ser Gly Leu Cys Phe Asp Ala Ser Gly Val Ala Asp Lys Gly Ala Cys 2645 2650 2655 Ile Phe Asn Ser Ala Cys Thr Thr Leu Ser Gly Leu Gly Gly Thr Ala 2660 2665 2670 Val Tyr Cys Tyr Lys Asn Gly Leu Val Glu Gly Ala Lys Leu Tyr Ser 2675 2680 2685 Glu Leu Ala Pro His Ser Tyr Tyr Lys Met Val Asp Gly Asn Ala Val 2690 2695 2700 Ser Leu Pro Glu Ile Ile Ser Arg Gly Phe Gly Ile Arg Thr Ile Arg 2705 2710 2715 2720 Thr Lys Ala Met Thr Tyr Cys Arg Val Gly Gln Cys Val Gln Ser Ala 2725 2730 2735 Glu Gly Val Cys Phe Gly Ala Asp Arg Phe Phe Val Tyr Asn Ala Glu 2740 2745 2750 Ser Gly Ser Asp Phe Val Cys Gly Thr Gly Leu Phe Thr Leu Leu Met 2755 2760 2765 Asn Val Ile Ser Val Phe Ser Lys Thr Val Pro Val Thr Val Leu Ser 2770 2775 2780 Gly Gln Ile Leu Phe Asn Cys Ile Ile Ala Phe Ala Ala Val Ala Val 2785 2790 2795 2800 Cys Phe Leu Phe Thr Lys Phe Lys Arg Met Phe Gly Asp Met Ser Val 2805 2810 2815 Gly Val Phe Thr Val Gly Ala Cys Thr Leu Leu Asn Asn Val Ser Tyr 2820 2825 2830 Ile Val Thr Gln Asn Thr Leu Gly Met Leu Gly Tyr Ala Thr Leu Tyr 2835 2840 2845 Phe Leu Cys Thr Lys Gly Val Arg Tyr Met Trp Ile Trp His Leu Gly 2850 2855 2860 Phe Leu Ile Ser Tyr Ile Leu Ile Ala Pro Trp Trp Val Leu Met Val 2865 2870 2875 2880 Tyr Ala Phe Ser Ala Ile Phe Glu Phe Met Pro Asn Leu Phe Lys Leu 2885 2890 2895 Lys Val Ser Thr Gln Leu Phe Glu Gly Asp Lys Phe Val Gly Ser Phe 2900 2905 2910 Glu Asn Ala Ala Ala Gly Thr Phe Val Leu Asp Met His Ala Tyr Glu 2915 2920 2925 Arg Leu Ala Asn Ser Ile Ser Thr Glu Lys Leu Arg Gln Tyr Ala Ser 2930 2935 2940 Thr Tyr Asn Lys Tyr Lys Tyr Tyr Ser Gly Ser Ala Ser Glu Ala Asp 2945 2950 2955 2960 Tyr Arg Leu Ala Cys Phe Ala His Leu Ala Lys Ala Met Met Asp Tyr 2965 2970 2975 Ala Ser Asn His Asn Asp Thr Leu Tyr Thr Pro Pro Thr Val Ser Tyr 2980 2985 2990 Asn Ser Thr Leu Gln Ala Gly Leu Arg Lys Met Ala Gln Pro Ser Gly 2995 3000 3005 Val Val Glu Lys Cys Ile Val Arg Val Cys Tyr Gly Asn Met Ala Leu 3010 3015 3020 Asn Gly Leu Trp Leu Gly Asp Thr Val Ile Cys Pro Arg His Val Ile 3025 3030 3035 3040 Ala Ser Ser Thr Thr Ser Thr Ile Asp Tyr Asp Tyr Ala Leu Ser Val 3045 3050 3055 Leu Arg Leu His Asn Phe Ser Ile Ser Ser Gly Asn Val Phe Leu Gly 3060 3065 3070 Val Val Gly Val Thr Met Arg Gly Ala Leu Leu Gln Ile Lys Val Asn 3075 3080 3085 Gln Asn Asn Val His Thr Pro Lys Tyr Thr Tyr Arg Thr Val Arg Pro 3090 3095 3100 Gly Glu Ser Phe Asn Ile Leu Ala Cys Tyr Asp Gly Ser Ala Ala Gly 3105 3110 3115 3120 Val Tyr Gly Val Asn Met Arg Ser Asn Tyr Thr Ile Arg Gly Ser Phe 3125 3130 3135 Ile Asn Gly Ala Cys Gly Ser Pro Gly Tyr Asn Ile Asn Asn Gly Thr 3140 3145 3150 Val Glu Phe Cys Tyr Leu His Gln Leu Glu Leu Gly Ser Gly Cys His 3155 3160 3165 Val Gly Ser Asp Leu Asp Gly Val Met Tyr Gly Gly Tyr Glu Asp Gln 3170 3175 3180 Pro Thr Leu Gln Val Glu Gly Ala Ser Ser Leu Phe Thr Glu Asn Val 3185 3190 3195 3200 Leu Ala Phe Leu Tyr Ala Ala Leu Ile Asn Gly Ser Thr Trp Trp Leu 3205 3210 3215 Ser Ser Ser Arg Ile Ala Val Asp Arg Phe Asn Glu Trp Ala Val His 3220 3225 3230 Asn Gly Met Thr Thr Val Val Asn Thr Asp Cys Phe Ser Ile Leu Ala 3235 3240 3245 Ala Lys Thr Gly Val Asp Val Gln Arg Leu Leu Ala Ser Ile Gln Ser 3250 3255 3260 Leu His Lys Asn Phe Gly Gly Lys Gln Ile Leu Gly Tyr Thr Ser Leu 3265 3270 3275 3280 Thr Asp Glu Phe Thr Thr Gly Glu Val Ile Arg Gln Met Tyr Gly Val 3285 3290 3295 Asn Leu Gln Ser Gly Tyr Val Ser Arg Ala Cys Arg Asn Val Leu Leu 3300 3305 3310 Val Gly Ser Phe Leu Thr Phe Phe Trp Ser Glu Leu Val Ser Tyr Thr 3315 3320 3325 Lys Phe Phe Trp Val Asn Pro Gly Tyr Val Thr Pro Met Phe Ala Cys 3330 3335 3340 Leu Ser Leu Leu Ser Ser Leu Leu Met Phe Thr Leu Lys His Lys Thr 3345 3350 3355 3360 Leu Phe Phe Gln Val Phe Leu Ile Pro Ala Leu Ile Val Thr Ser Cys 3365 3370 3375 Ile Asn Leu Ala Phe Asp Val Glu Val Tyr Asn Tyr Leu Ala Glu His 3380 3385 3390 Phe Asp Tyr His Val Ser Leu Met Gly Phe Asn Ala Gln Gly Leu Val 3395 3400 3405 Asn Ile Phe Val Cys Phe Val Val Thr Ile Leu His Gly Thr Tyr Thr 3410 3415 3420 Trp Arg Phe Phe Asn Thr Pro Val Ser Ser Val Thr Tyr Val Val Ala 3425 3430 3435 3440 Leu Leu Thr Ala Ala Tyr Asn Tyr Phe Tyr Ala Ser Asp Ile Leu Ser 3445 3450 3455 Cys Ala Met Thr Leu Phe Ala Ser Val Thr Gly Asn Trp Phe Val Gly 3460 3465 3470 Ala Val Cys Tyr Lys Ala Ala Val Tyr Met Ala Leu Arg Phe Pro Thr 3475 3480 3485 Phe Val Ala Ile Phe Gly Asp Ile Lys Ser Val Met Phe Cys Tyr Leu 3490 3495 3500 Val Leu Gly Tyr Phe Thr Cys Cys Phe Tyr Gly Ile Leu Tyr Trp Phe 3505 3510 3515 3520 Asn Arg Phe Phe Lys Val Ser Val Gly Val Tyr Asp Tyr Thr Val Ser 3525 3530 3535 Ala Ala Glu Phe Lys Tyr Met Val Ala Asn Gly Leu Arg Ala Pro Thr 3540 3545 3550 Gly Thr Leu Asp Ser Leu Leu Leu Ser Ala Lys Leu Ile Gly Ile Gly 3555 3560 3565 Gly Glu Arg Asn Ile Lys Ile Ser Ser Val Gln Ser Lys Leu Thr Asp 3570 3575 3580 Ile Lys Cys Ser Asn Val Val Leu Leu Gly Cys Leu Ser Ser Met Asn 3585 3590 3595 3600 Val Ser Ala Asn Ser Thr Glu Trp Ala Tyr Cys Val Asp Leu His Asn 3605 3610 3615 Lys Ile Asn Leu Cys Asn Asp Pro Glu Lys Ala Gln Glu Met Leu Leu 3620 3625 3630 Ala Leu Leu Ala Phe Phe Leu Ser Lys Asn Ser Ala Phe Gly Leu Asp 3635 3640 3645 Asp Leu Leu Glu Ser Tyr Phe Asn Asp Asn Ser Met Leu Gln Ser Val 3650 3655 3660 Ala Ser Thr Tyr Val Gly Leu Pro Ser Tyr Val Ile Tyr Glu Asn Ala 3665 3670 3675 3680 Arg Gln Gln Tyr Glu Asp Ala Val Asn Asn Gly Ser Pro Pro Gln Leu 3685 3690 3695 Val Lys Gln Leu Arg His Ala Met Asn Val Ala Lys Ser Glu Phe Asp 3700 3705 3710 Arg Glu Ala Ser Thr Gln Arg Lys Leu Asp Arg Met Ala Glu Gln Ala 3715 3720 3725 Ala Ala Gln Met Tyr Lys Glu Ala Arg Ala Val Asn Arg Lys Ser Lys 3730 3735 3740 Val Val Ser Ala Met His Ser Leu Leu Phe Gly Met Leu Arg Arg Leu 3745 3750 3755 3760 Asp Met Ser Ser Val Asp Thr Ile Leu Asn Leu Ala Lys Asp Gly Val 3765 3770 3775 Val Pro Leu Ser Val Ile Pro Ala Val Ser Ala Thr Lys Leu Asn Ile 3780 3785 3790 Val Thr Ser Asp Ile Asp Ser Tyr Asn Arg Ile Gln Arg Glu Gly Cys 3795 3800 3805 Val His Tyr Ala Gly Thr Ile Trp Asn Ile Ile Asp Ile Lys Asp Asn 3810 3815 3820 Asp Gly Lys Val Val His Val Lys Glu Val Thr Ala Gln Asn Ala Glu 3825 3830 3835 3840 Ser Leu Ser Trp Pro Leu Val Leu Gly Cys Glu Arg Ile Val Lys Leu 3845 3850 3855 Gln Asn Asn Glu Ile Ile Pro Gly Lys Leu Lys Gln Arg Ser Ile Lys 3860 3865 3870 Ala Glu Gly Asp Gly Ile Val Gly Glu Gly Lys Ala Leu Tyr Asn Asn 3875 3880 3885 Glu Gly Gly Arg Thr Phe Met Tyr Ala Phe Ile Ser Asp Lys Pro Asp 3890 3895 3900 Leu Arg Val Val Lys Trp Glu Phe Asp Gly Gly Cys Asn Thr Ile Glu 3905 3910 3915 3920 Leu Glu Pro Pro Arg Lys Phe Leu Val Asp Ser Pro Asn Gly Ala Gln 3925 3930 3935 Ile Lys Tyr Leu Tyr Phe Val Arg Asn Leu Asn Thr Leu Arg Arg Gly 3940 3945 3950 Ala Val Leu Gly Tyr Ile Gly Ala Thr Val Arg Leu Gln Ala Gly Lys 3955 3960 3965 Gln Thr Glu Gln Ala Ile Asn Ser Ser Leu Leu Thr Leu Cys Ala Phe 3970 3975 3980 Ala Val Asp Pro Ala Lys Thr Tyr Ile Asp Ala Val Lys Ser Gly His 3985 3990 3995 4000 Lys Pro Val Gly Asn Cys Val Lys Met Leu Ala Asn Gly Ser Gly Asn 4005 4010 4015 Gly Gln Ala Val Thr Asn Gly Val Glu Ala Ser Thr Asn Gln Asp Ser 4020 4025 4030 Tyr Gly Gly Ala Ser Val Cys Leu Tyr Cys Arg Ala His Val Glu His 4035 4040 4045 Pro Ser Met Asp Gly Phe Cys Arg Leu Lys Gly Lys Tyr Val Gln Val 4050 4055 4060 Pro Leu Gly Thr Val Asp Pro Ile Arg Phe Val Leu Glu Asn Asp Val 4065 4070 4075 4080 Cys Lys Val Cys Gly Cys Trp Leu Ala Asn Gly Cys Thr Cys Asp Arg 4085 4090 4095 Ser Ile Met Gln Ser Thr Asp Tyr Gly Leu Phe Lys Arg Val Arg Gly 4100 4105 4110 Ser Ser Ala Ala Arg Leu Glu Pro Cys Asn Gly Thr Asp Thr Gln His 4115 4120 4125 Val Tyr Arg Ala Phe Asp Ile Tyr Asn Lys Asp Val Ala Cys Leu Gly 4130 4135 4140 Lys Phe Leu Lys Val Asn Cys Val Arg Leu Lys Asn Leu Asp Lys His 4145 4150 4155 4160 Asp Ala Phe Tyr Val Val Lys Arg Cys Thr Lys Ser Ala Met Glu His 4165 4170 4175 Glu Gln Ser Ile Tyr Ser Arg Leu Glu Lys Cys Gly Ala Val Ala Glu 4180 4185 4190 His Asp Phe Phe Thr Trp Lys Asp Gly Arg Ala Ile Tyr Gly Asn Val 4195 4200 4205 Cys Arg Lys Asp Leu Thr Glu Tyr Thr Met Met Asp Leu Cys Tyr Ala 4210 4215 4220 Leu Arg Asn Phe Asp Glu Asn Asn Cys Asp Val Leu Lys Ser Ile Leu 4225 4230 4235 4240 Ile Lys Val Gly Ala Cys Glu Glu Ser Tyr Phe Asn Asn Lys Val Trp 4245 4250 4255 Phe Asp Pro Val Glu Asn Glu Asp Ile His Arg Val Tyr Ala Leu Leu 4260 4265 4270 Gly Thr Ile Val Ser Arg Ala Met Leu Lys Cys Val Lys Phe Cys Asp 4275 4280 4285 Ala Met Val Glu Gln Gly Ile Val Gly Val Val Thr Leu Asp Asn Gln 4290 4295 4300 Asp Leu Asn Gly Asp Phe Tyr Asp Phe Gly Asp Phe Thr Cys Ser Ile 4305 4310 4315 4320 Lys Gly Met Gly Ile Pro Ile Cys Thr Ser Tyr Tyr Ser Tyr Met Met 4325 4330 4335 Pro Val Met Gly Met Thr Asn Cys Leu Ala Ser Glu Cys Phe Val Lys 4340 4345 4350 Ser Asp Ile Phe Gly Glu Asp Phe Lys Ser Tyr Asp Leu Leu Glu Tyr 4355 4360 4365 Asp Phe Thr Glu His Lys Thr Ala Leu Phe Asn Lys Tyr Phe Lys Tyr 4370 4375 4380 Trp Gly Leu Gln Tyr His Pro Asn Cys Val Asp Cys Ser Asp Glu Gln 4385 4390 4395 4400 Cys Ile Val His Cys Ala Asn Phe Asn Thr Leu Phe Ser Thr Thr Ile 4405 4410 4415 Pro Ile Thr Ala Phe Gly Pro Leu Cys Arg Lys Cys Trp Ile Asp Gly 4420 4425 4430 Val Pro Leu Val Thr Thr Ala Gly Tyr His Phe Lys Gln Leu Gly Ile 4435 4440 4445 Val Trp Asn Asn Asp Leu Asn Leu His Ser Ser Arg Leu Ser Ile Asn 4450 4455 4460 Glu Leu Leu Gln Phe Cys Ser Asp Pro Ala Leu Leu Ile Ala Ser Ser 4465 4470 4475 4480 Pro Ala Leu Val Asp Gln Arg Thr Val Cys Phe Ser Val Ala Ala Leu 4485 4490 4495 Gly Thr Gly Met Thr Asn Gln Thr Val Lys Pro Gly His Phe Asn Lys 4500 4505 4510 Glu Phe Tyr Asp Phe Leu Leu Glu Gln Gly Phe Phe Ser Glu Gly Ser 4515 4520 4525 Glu Leu Thr Leu Lys His Phe Phe Phe Ala Gln Lys Gly Asp Ala Ala 4530 4535 4540 Val Lys Asp Phe Asp Tyr Tyr Arg Tyr Asn Arg Pro Thr Val Leu Asp 4545 4550 4555 4560 Ile Cys Gln Ala Arg Val Val Tyr Gln Ile Val Gln Arg Tyr Phe Asp 4565 4570 4575 Ile Tyr Glu Gly Gly Cys Ile Thr Ala Lys Glu Val Val Val Thr Asn 4580 4585 4590 Leu Asn Lys Ser Ala Gly Tyr Pro Leu Asn Lys Phe Gly Lys Ala Gly 4595 4600 4605 Leu Tyr Tyr Glu Ser Leu Ser Tyr Glu Glu Gln Asp Glu Leu Tyr Ala 4610 4615 4620 Tyr Thr Lys Arg Asn Ile Leu Pro Thr Met Thr Gln Leu Asn Leu Lys 4625 4630 4635 4640 Tyr Ala Ile Ser Gly Lys Glu Arg Ala Arg Thr Val Gly Gly Val Ser 4645 4650 4655 Leu Leu Ser Thr Met Thr Thr Arg Gln Tyr His Gln Lys His Leu Lys 4660 4665 4670 Ser Ile Val Asn Thr Arg Gly Ala Ser Val Val Ile Gly Thr Thr Lys 4675 4680 4685 Phe Tyr Gly Gly Trp Asp Asn Met Leu Lys Asn Leu Ile Asp Gly Val 4690 4695 4700 Glu Asn Pro Cys Leu Met Gly Trp Asp Tyr Pro Lys Cys Asp Arg Ala 4705 4710 4715 4720 Leu Pro Asn Met Ile Arg Met Ile Ser Ala Met Ile Leu Gly Ser Lys 4725 4730 4735 His Thr Thr Cys Cys Ser Ser Thr Asp Arg Phe Phe Arg Leu Cys Asn 4740 4745 4750 Glu Leu Ala Gln Val Leu Thr Glu Val Val Tyr Ser Asn Gly Gly Phe 4755 4760 4765 Tyr Leu Lys Pro Gly Gly Thr Thr Ser Gly Asp Ala Thr Thr Ala Tyr 4770 4775 4780 Ala Asn Ser Val Phe Asn Ile Phe Gln Ala Val Ser Ala Asn Val Asn 4785 4790 4795 4800 Lys Leu Leu Ser Val Asp Ser Asn Val Cys His Asn Leu Glu Val Lys 4805 4810 4815 Gln Leu Gln Arg Lys Leu Tyr Glu Cys Cys Tyr Arg Ser Thr Thr Val 4820 4825 4830 Asp Asp Gln Phe Val Val Glu Tyr Tyr Gly Tyr Leu Arg Lys His Phe 4835 4840 4845 Ser Met Met Ile Leu Ser Asp Asp Gly Val Val Cys Tyr Asn Asn Asp 4850 4855 4860 Tyr Ala Ser Leu Gly Tyr Val Ala Asp Leu Asn Ala Phe Lys Ala Val 4865 4870 4875 4880 Leu Tyr Tyr Gln Asn Asn Val Phe Met Ser Ala Ser Lys Cys Trp Ile 4885 4890 4895 Glu Pro Asp Ile Asn Lys Gly Pro His Glu Phe Cys Ser Gln His Thr 4900 4905 4910 Met Gln Ile Val Asp Lys Asp Gly Thr Tyr Tyr Leu Pro Tyr Pro Asp 4915 4920 4925 Pro Ser Arg Ile Leu Ser Ala Gly Val Phe Val Asp Asp Val Val Lys 4930 4935 4940 Thr Asp Ala Val Val Leu Leu Glu Arg Tyr Val Ser Leu Ala Ile Asp 4945 4950 4955 4960 Ala Tyr Pro Leu Ser Lys His Glu Asn Pro Glu Tyr Lys Lys Val Phe 4965 4970 4975 Tyr Val Leu Leu Asp Trp Val Lys His Leu Tyr Lys Thr Leu Asn Ala 4980 4985 4990 Gly Val Leu Glu Ser Phe Ser Val Thr Leu Leu Glu Asp Ser Thr Ala 4995 5000 5005 Lys Phe Trp Asp Glu Ser Phe Tyr Ala Asn Met Tyr Glu Lys Ser Ala 5010 5015 5020 Val Leu Gln Ser Ala Gly Leu Cys Val Val Cys Gly Ser Gln Thr Val 5025 5030 5035 5040 Leu Arg Cys Gly Asp Cys Leu Arg Arg Pro Met Leu Cys Thr Lys Cys 5045 5050 5055 Ala Tyr Asp His Val Ile Gly Thr Thr His Lys Phe Ile Leu Ala Ile 5060 5065 5070 Thr Pro Tyr Val Cys Cys Ala Ser Asp Cys Gly Val Asn Asp Val Thr 5075 5080 5085 Lys Leu Tyr Leu Gly Gly Leu Ser Tyr Trp Cys His Asp His Lys Pro 5090 5095 5100 Arg Leu Ala Phe Pro Leu Cys Ser Ala Gly Asn Val Phe Gly Leu Tyr 5105 5110 5115 5120 Lys Asn Ser Ala Thr Gly Ser Pro Asp Val Glu Asp Phe Asn Arg Ile 5125 5130 5135 Ala Thr Ser Asp Trp Thr Asp Val Ser Asp Tyr Arg Leu Ala Asn Asp 5140 5145 5150 Val Lys Asp Ser Leu Arg Leu Phe Ala Ala Glu Thr Ile Lys Ala Lys 5155 5160 5165 Glu Glu Ser Val Lys Ser Ser Tyr Ala Cys Ala Thr Leu His Glu Val 5170 5175 5180 Val Gly Pro Lys Glu Leu Leu Leu Lys Trp Glu Val Gly Arg Pro Lys 5185 5190 5195 5200 Pro Pro Leu Asn Arg Asn Ser Val Phe Thr Cys Tyr His Ile Thr Lys 5205 5210 5215 Asn Thr Lys Phe Gln Ile Gly Glu Phe Val Phe Glu Lys Ala Glu Tyr 5220 5225 5230 Asp Asn Asp Ala Val Thr Tyr Lys Thr Thr Ala Thr Thr Lys Leu Val 5235 5240 5245 Pro Gly Met Val Phe Val Leu Thr Ser His Asn Val Gln Pro Leu Arg 5250 5255 5260 Ala Pro Thr Ile Ala Asn Gln Glu Arg Tyr Ser Thr Ile His Lys Leu 5265 5270 5275 5280 His Pro Ala Phe Asn Ile Pro Glu Ala Tyr Ser Ser Leu Val Pro Tyr 5285 5290 5295 Tyr Gln Leu Ile Gly Lys Gln Lys Ile Thr Thr Ile Gln Gly Pro Pro 5300 5305 5310 Gly Ser Gly Lys Ser His Cys Val Ile Gly Leu Gly Leu Tyr Tyr Pro 5315 5320 5325 Gly Ala Arg Ile Val Phe Thr Ala Cys Ser His Ala Ala Val Asp Ser 5330 5335 5340 Leu Cys Val Lys Ala Ser Thr Ala Tyr Ser Asn Asp Lys Cys Ser Arg 5345 5350 5355 5360 Ile Ile Pro Gln Arg Ala Arg Val Glu Cys Tyr Asp Gly Phe Lys Ser 5365 5370 5375 Asn Asn Thr Ser Ala Gln Tyr Leu Phe Ser Thr Val Asn Ala Leu Pro 5380 5385 5390 Glu Cys Asn Ala Asp Ile Val Val Val Asp Glu Val Ser Met Cys Thr 5395 5400 5405 Asn Tyr Asp Leu Ser Val Ile Asn Gln Arg Ile Ser Tyr Arg His Val 5410 5415 5420 Val Tyr Val Gly Asp Pro Gln Gln Leu Pro Ala Pro Arg Val Met Ile 5425 5430 5435 5440 Ser Arg Gly Thr Leu Glu Pro Lys Asp Tyr Asn Val Val Thr Gln Arg 5445 5450 5455 Met Cys Ala Leu Lys Pro Asp Val Phe Leu His Lys Cys Tyr Arg Cys 5460 5465 5470 Pro Ala Glu Ile Val Arg Thr Val Ser Glu Met Val Tyr Glu Asn Gln 5475 5480 5485 Phe Ile Pro Val His Pro Asp Ser Lys Gln Cys Phe Lys Ile Phe Cys 5490 5495 5500 Lys Gly Asn Val Gln Val Asp Asn Gly Ser Ser Ile Asn Arg Arg Gln 5505 5510 5515 5520 Leu Asp Val Val Arg Met Phe Leu Ala Lys Asn Pro Arg Trp Ser Lys 5525 5530 5535 Ala Val Phe Ile Ser Pro Tyr Asn Ser Gln Asn Tyr Val Ala Ser Arg 5540 5545 5550 Met Leu Gly Leu Gln Ile Gln Thr Val Asp Ser Ser Gln Gly Ser Glu 5555 5560 5565 Tyr Asp Tyr Val Ile Tyr Thr Gln Thr Ser Asp Thr Ala His Ala Cys 5570 5575 5580 Asn Val Asn Arg Phe Asn Val Ala Ile Thr Arg Ala Lys Lys Gly Ile 5585 5590 5595 5600 Leu Cys Ile Met Cys Asp Arg Ser Leu Phe Asp Val Leu Lys Phe Phe 5605 5610 5615 Glu Leu Lys Leu Ser Asp Leu Gln Ala Asn Glu Gly Cys Gly Leu Phe 5620 5625 5630 Lys Asp Cys Ser Arg Gly Asp Asp Leu Leu Pro Pro Ser His Ala Asn 5635 5640 5645 Thr Phe Met Ser Leu Ala Asp Asn Phe Lys Thr Asp Gln Asp Leu Ala 5650 5655 5660 Val Gln Ile Gly Val Asn Gly Pro Ile Lys Tyr Glu His Val Ile Ser 5665 5670 5675 5680 Phe Met Gly Phe Arg Phe Asp Ile Asn Ile Pro Asn His His Thr Leu 5685 5690 5695 Phe Cys Thr Arg Asp Phe Ala Met Arg Asn Val Arg Gly Trp Leu Gly 5700 5705 5710 Phe Asp Val Glu Gly Ala His Val Val Gly Ser Asn Val Gly Thr Asn 5715 5720 5725 Val Pro Leu Gln Leu Gly Phe Ser Asn Gly Val Asp Phe Val Val Arg 5730 5735 5740 Pro Glu Gly Cys Val Val Thr Glu Ser Gly Asp Tyr Ile Lys Pro Val 5745 5750 5755 5760 Arg Ala Arg Ala Pro Pro Gly Glu Gln Phe Ala His Leu Leu Pro Leu 5765 5770 5775 Leu Lys Arg Gly Gln Pro Trp Asp Val Val Arg Lys Arg Ile Val Gln 5780 5785 5790 Met Cys Ser Asp Tyr Leu Ala Asn Leu Ser Asp Ile Leu Ile Phe Val 5795 5800 5805 Leu Trp Ala Gly Gly Leu Glu Leu Thr Thr Met Arg Tyr Phe Val Lys 5810 5815 5820 Ile Gly Pro Ser Lys Ser Cys Asp Cys Gly Lys Val Ala Thr Cys Tyr 5825 5830 5835 5840 Asn Ser Ala Leu His Thr Tyr Cys Cys Phe Lys His Ala Leu Gly Cys 5845 5850 5855 Asp Tyr Leu Tyr Asn Pro Tyr Cys Ile Asp Ile Gln Gln Trp Gly Tyr 5860 5865 5870 Lys Gly Ser Leu Ser Leu Asn His His Glu His Cys Asn Val His Arg 5875 5880 5885 Asn Glu His Val Ala Ser Gly Asp Ala Ile Met Thr Arg Cys Leu Ala 5890 5895 5900 Ile His Asp Cys Phe Val Lys Asn Val Asp Trp Ser Ile Thr Tyr Pro 5905 5910 5915 5920 Phe Ile Gly Asn Glu Ala Val Ile Asn Lys Ser Gly Arg Ile Val Gln 5925 5930 5935 Ser His Thr Met Arg Ser Val Leu Lys Leu Tyr Asn Pro Lys Ala Ile 5940 5945 5950 Tyr Asp Ile Gly Asn Pro Lys Gly Ile Arg Cys Ala Val Thr Asp Ala 5955 5960 5965 Lys Trp Phe Cys Phe Asp Lys Asn Pro Thr Asn Ser Asn Val Lys Thr 5970 5975 5980 Leu Glu Tyr Asp Tyr Ile Thr His Gly Gln Phe Asp Gly Leu Cys Leu 5985 5990 5995 6000 Phe Trp Asn Cys Asn Val Asp Met Tyr Pro Glu Phe Ser Val Val Cys 6005 6010 6015 Arg Phe Asp Thr Arg Cys Arg Ser Pro Leu Asn Leu Glu Gly Cys Asn 6020 6025 6030 Gly Gly Ser Leu Tyr Val Asn Asn His Ala Phe His Thr Pro Ala Phe 6035 6040 6045 Asp Lys Arg Ala Phe Ala Lys Leu Lys Pro Met Pro Phe Phe Phe Tyr 6050 6055 6060 Asp Asp Thr Glu Cys Asp Lys Leu Gln Asp Ser Ile Asn Tyr Val Pro 6065 6070 6075 6080 Leu Arg Ala Ser Asn Cys Ile Thr Lys Cys Asn Val Gly Gly Ala Val 6085 6090 6095 Cys Ser Lys His Cys Ala Met Tyr His Ser Tyr Val Asn Ala Tyr Asn 6100 6105 6110 Thr Phe Thr Ser Ala Gly Phe Thr Ile Trp Val Pro Thr Ser Phe Asp 6115 6120 6125 Thr Tyr Asn Leu Trp Gln Thr Phe Ser Asn Asn Leu Gln Gly Leu Glu 6130 6135 6140 Asn Ile Ala Phe Asn Val Val Lys Lys Gly Ser Phe Val Gly Ala Glu 6145 6150 6155 6160 Gly Glu Leu Pro Val Ala Val Val Asn Asp Lys Val Leu Val Arg Asp 6165 6170 6175 Gly Thr Val Asp Thr Leu Val Phe Thr Asn Lys Thr Ser Leu Pro Thr 6180 6185 6190 Asn Val Ala Phe Glu Leu Tyr Ala Lys Arg Lys Val Gly Leu Thr Pro 6195 6200 6205 Pro Ile Thr Ile Leu Arg Asn Leu Gly Val Val Cys Thr Ser Lys Cys 6210 6215 6220 Val Ile Trp Asp Tyr Glu Ala Glu Arg Pro Leu Thr Thr Phe Thr Lys 6225 6230 6235 6240 Asp Val Cys Lys Tyr Thr Asp Phe Glu Gly Asp Val Cys Thr Leu Phe 6245 6250 6255 Asp Asn Ser Ile Val Gly Ser Leu Glu Arg Phe Ser Met Thr Gln Asn 6260 6265 6270 Ala Val Leu Met Ser Leu Thr Ala Val Lys Lys Leu Thr Gly Ile Lys 6275 6280 6285 Leu Thr Tyr Gly Tyr Leu Asn Gly Val Pro Val Asn Thr His Glu Asp 6290 6295 6300 Lys Pro Phe Thr Trp Tyr Ile Tyr Thr Arg Lys Asn Gly Lys Phe Glu 6305 6310 6315 6320 Asp His Pro Asp Gly Tyr Phe Thr Gln Gly Arg Thr Thr Ala Asp Phe 6325 6330 6335 Ser Pro Arg Ser Asp Met Glu Lys Asp Phe Leu Ser Met Asp Met Gly 6340 6345 6350 Leu Phe Ile Asn Lys Tyr Gly Leu Glu Asp Tyr Gly Phe Glu His Val 6355 6360 6365 Val Tyr Gly Asp Val Ser Lys Thr Thr Leu Gly Gly Leu His Leu Leu 6370 6375 6380 Ile Ser Gln Val Arg Leu Ala Cys Met Gly Val Leu Lys Ile Asp Glu 6385 6390 6395 6400 Phe Val Ser Ser Asn Asp Ser Thr Leu Lys Ser Cys Thr Val Thr Tyr 6405 6410 6415 Ala Asp Asn Pro Ser Ser Lys Met Val Cys Thr Tyr Met Asp Leu Leu 6420 6425 6430 Leu Asp Asp Phe Val Ser Ile Leu Lys Ser Leu Asp Leu Gly Val Val 6435 6440 6445 Ser Lys Val His Glu Val Met Val Asp Cys Lys Met Trp Arg Trp Met 6450 6455 6460 Leu Trp Cys Lys Asp His Lys Leu Gln Thr Phe Tyr Pro Gln Leu Gln 6465 6470 6475 6480 Ala Ser Glu Trp Lys Cys Gly Tyr Ser Met Pro Ser Ile Tyr Lys Ile 6485 6490 6495 Gln Arg Met Cys Leu Glu Pro Cys Asn Leu Tyr Asn Tyr Gly Ala Gly 6500 6505 6510 Ile Lys Leu Pro Asp Gly Ile Met Phe Asn Val Val Lys Tyr Thr Gln 6515 6520 6525 Leu Cys Gln Tyr Leu Asn Ser Thr Thr Met Cys Val Pro His His Met 6530 6535 6540 Arg Val Leu His Leu Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly 6545 6550 6555 6560 Thr Ala Val Leu Arg Arg Trp Leu Pro Leu Asp Ala Ile Ile Val Asp 6565 6570 6575 Asn Asp Ser Val Asp Tyr Val Ser Asp Ala Asp Tyr Ser Val Thr Gly 6580 6585 6590 Asp Cys Ser Thr Leu Tyr Leu Ser Asp Lys Phe Asp Leu Val Ile Ser 6595 6600 6605 Asp Met Tyr Asp Gly Lys Ile Lys Ser Cys Asp Gly Glu Asn Val Ser 6610 6615 6620 Lys Glu Gly Phe Phe Pro Tyr Ile Asn Gly Val Ile Thr Glu Lys Leu 6625 6630 6635 6640 Ala Leu Gly Gly Thr Val Ala Ile Lys Val Thr Glu Phe Ser Trp Asn 6645 6650 6655 Lys Lys Leu Tyr Glu Leu Ile Gln Arg Phe Glu Tyr Trp Thr Met Phe 6660 6665 6670 Cys Thr Ser Val Asn Thr Ser Ser Ser Glu Ala Phe Leu Ile Gly Val 6675 6680 6685 His Tyr Leu Gly Asp Phe Ala Ser Gly Ala Val Ile Asp Gly Asn Thr 6690 6695 6700 Met His Ala Asn Tyr Ile Phe Trp Arg Asn Ser Thr Ile Met Thr Met 6705 6710 6715 6720 Ser Tyr Asn Ser Val Leu Asp Leu Ser Lys Phe Asn Cys Lys His Lys 6725 6730 6735 Ala Thr Val Val Ile Asn Leu Lys Asp Ser Ser Ile Ser Asp Val Val 6740 6745 6750 Leu Gly Leu Leu Lys Asn Gly Lys Leu Leu Val Arg Asn Asn Asp Ala 6755 6760 6765 Ile Cys Gly Phe Ser Asn His Leu Val Asn Val Asn Lys 6770 6775 6780 <210> 17 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 ORF3 <400> 17 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Asp Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 18 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 envelope protein <400> 18 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Pro Ser Thr Val Ile Asp Val 65 70 75 <210> 19 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 membrane protein <400> 19 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 20 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 nucleocapsid protein <400> 20 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 21 <211> 28038 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112- p10 complete genome <400> 21 Ala Cys Thr Thr Ala Ala Ala Gly Ala Gly Ala Thr Thr Thr Thr Cys 1 5 10 15 Thr Ala Thr Cys Thr Ala Cys Gly Gly Ala Thr Ala Gly Thr Thr Ala 20 25 30 Gly Cys Thr Cys Thr Thr Thr Thr Thr Cys Thr Ala Gly Ala Cys Thr 35 40 45 Cys Thr Thr Gly Thr Cys Thr Ala Cys Thr Cys Ala Ala Thr Thr Cys 50 55 60 Ala Ala Cys Thr Ala Ala Ala Cys Gly Ala Ala Ala Thr Thr Thr Thr 65 70 75 80 Gly Thr Cys Cys Thr Thr Cys Cys Gly Gly Cys Cys Gly Cys Ala Thr 85 90 95 Gly Thr Cys Cys Ala Thr Gly Cys Thr Gly Cys Thr Gly Gly Ala Ala 100 105 110 Gly Cys Thr Gly Ala Cys Gly Thr Gly Gly Ala Ala Thr Thr Thr Cys 115 120 125 Ala Thr Thr Ala Gly Gly Thr Thr Thr Gly Cys Thr Thr Ala Ala Gly 130 135 140 Thr Ala Gly Cys Cys Ala Thr Cys Gly Cys Ala Ala Gly Thr Gly Cys 145 150 155 160 Thr Gly Thr Gly Cys Thr Gly Thr Cys Cys Thr Cys Thr Ala Gly Thr 165 170 175 Thr Cys Cys Thr Gly Gly Thr Thr Gly Gly Cys Gly Thr Thr Cys Cys 180 185 190 Gly Thr Cys Gly Cys Cys Thr Thr Cys Thr Ala Cys Ala Thr Ala Cys 195 200 205 Thr Ala Gly Ala Cys Ala Ala Ala Cys Ala Gly Cys Cys Thr Thr Cys 210 215 220 Cys Thr Cys Cys Gly Gly Thr Thr Cys Cys Gly Thr Cys Thr Gly Gly 225 230 235 240 Gly Gly Gly Thr Thr Gly Thr Gly Thr Gly Gly Ala Thr Ala Ala Cys 245 250 255 Thr Ala Gly Thr Thr Cys Cys Gly Thr Cys Thr Ala Gly Thr Thr Thr 260 265 270 Gly Ala Ala Ala Cys Cys Ala Gly Thr Ala Ala Cys Thr Gly Thr Cys 275 280 285 Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Ala Gly Cys Ala Ala Cys 290 295 300 Cys Ala Thr Gly Thr Thr Ala Cys Ala Thr Thr Gly Gly Cys Thr Thr 305 310 315 320 Thr Thr Gly Cys Cys Ala Ala Thr Gly Ala Thr Gly Cys Ala Gly Ala 325 330 335 Ala Ala Thr Thr Thr Cys Ala Gly Cys Thr Thr Thr Thr Gly Gly Cys 340 345 350 Thr Thr Thr Thr Gly Cys Ala Cys Thr Gly Cys Thr Ala Gly Thr Gly 355 360 365 Ala Ala Gly Cys Cys Gly Thr Cys Thr Cys Ala Thr Ala Cys Thr Ala 370 375 380 Thr Thr Cys Thr Gly Ala Gly Gly Cys Cys Gly Cys Cys Gly Cys Thr 385 390 395 400 Ala Gly Thr Gly Gly Ala Thr Thr Thr Ala Thr Gly Cys Ala Ala Thr 405 410 415 Gly Cys Cys Gly Thr Thr Thr Cys Gly Thr Gly Thr Cys Cys Thr Thr 420 425 430 Cys Gly Ala Thr Cys Thr Cys Gly Cys Thr Gly Ala Cys Ala Cys Thr 435 440 445 Gly Thr Thr Gly Ala Gly Gly Gly Ala Thr Thr Gly Cys Thr Thr Cys 450 455 460 Cys Cys Gly Ala Ala Gly Ala Cys Thr Ala Thr Gly Thr Cys Ala Thr 465 470 475 480 Gly Gly Thr Gly Gly Thr Gly Gly Thr Cys Gly Gly Cys Ala Cys Thr 485 490 495 Ala Cys Cys Ala Ala Gly Cys Thr Thr Ala Gly Thr Gly Cys Gly Thr 500 505 510 Ala Thr Gly Thr Gly Gly Ala Cys Ala Cys Thr Thr Thr Thr Gly Gly 515 520 525 Thr Ala Gly Cys Cys Gly Cys Cys Cys Cys Ala Ala Ala Ala Ala Cys 530 535 540 Ala Thr Thr Thr Gly Thr Gly Gly Thr Thr Gly Gly Cys Thr Gly Thr 545 550 555 560 Thr Ala Thr Thr Thr Thr Cys Thr Ala Ala Cys Thr Gly Thr Ala Ala 565 570 575 Thr Thr Ala Cys Thr Thr Cys Cys Thr Cys Gly Ala Ala Gly Ala Gly 580 585 590 Thr Thr Ala Gly Ala Gly Cys Thr Thr Ala Cys Thr Thr Thr Thr Gly 595 600 605 Gly Thr Cys Gly Thr Cys Gly Thr Gly Gly Thr Gly Gly Thr Ala Ala 610 615 620 Cys Ala Thr Cys Gly Thr Gly Cys Cys Ala Gly Thr Thr Gly Ala Cys 625 630 635 640 Cys Ala Ala Thr Ala Cys Ala Thr Gly Thr Gly Thr Gly Gly Cys Gly 645 650 655 Cys Thr Gly Ala Cys Gly Gly Thr Ala Ala Ala Cys Cys Thr Gly Thr 660 665 670 Thr Cys Thr Thr Cys Ala Gly Gly Ala Ala Thr Cys Cys Gly Ala Ala 675 680 685 Thr Gly Gly Gly Ala Gly Thr Ala Thr Ala Cys Ala Gly Ala Thr Thr 690 695 700 Thr Cys Thr Thr Thr Gly Cys Thr Gly Ala Cys Thr Cys Cys Gly Ala 705 710 715 720 Ala Gly Ala Cys Gly Gly Thr Cys Ala Ala Cys Thr Cys Ala Ala Cys 725 730 735 Ala Thr Thr Gly Cys Thr Gly Gly Thr Ala Thr Cys Ala Cys Thr Thr 740 745 750 Ala Thr Gly Thr Gly Ala Ala Gly Gly Cys Cys Thr Gly Gly Ala Thr 755 760 765 Thr Gly Thr Ala Gly Ala Gly Cys Gly Ala Thr Cys Gly Gly Ala Thr 770 775 780 Gly Thr Cys Thr Cys Thr Thr Ala Thr Gly Cys Gly Ala Gly Thr Cys 785 790 795 800 Ala Gly Ala Ala Thr Thr Thr Ala Ala Cys Ala Thr Cys Thr Ala Thr 805 810 815 Thr Ala Ala Gly Thr Cys Thr Ala Thr Thr Ala Cys Thr Thr Ala Cys 820 825 830 Thr Gly Thr Thr Cys Ala Ala Cys Cys Thr Ala Thr Gly Ala Gly Cys 835 840 845 Ala Thr Ala Cys Thr Thr Thr Thr Cys Cys Thr Gly Ala Thr Gly Gly 850 855 860 Thr Ala Cys Thr Gly Cys Cys Ala Thr Gly Ala Ala Gly Gly Thr Thr 865 870 875 880 Gly Cys Ala Cys Gly Thr Ala Cys Thr Cys Cys Ala Ala Ala Gly Ala 885 890 895 Thr Thr Ala Ala Gly Ala Ala Gly Ala Cys Thr Gly Thr Thr Gly Thr 900 905 910 Cys Thr Thr Gly Thr Cys Thr Gly Ala Gly Cys Cys Ala Cys Thr Thr 915 920 925 Gly Cys Thr Ala Cys Thr Ala Thr Cys Thr Ala Cys Ala Gly Gly Gly 930 935 940 Ala Ala Ala Thr Thr Gly Gly Thr Thr Cys Thr Cys Cys Thr Thr Thr 945 950 955 960 Thr Gly Thr Gly Gly Ala Thr Ala Ala Thr Gly Gly Gly Ala Gly Cys 965 970 975 Gly Ala Thr Gly Cys Thr Cys Gly Thr Thr Cys Thr Ala Thr Cys Ala 980 985 990 Thr Thr Ala Ala Gly Ala Gly Ala Cys Cys Ala Gly Thr Gly Thr Thr 995 1000 1005 Cys Cys Thr Cys Cys Ala Cys Gly Cys Thr Thr Thr Thr Gly Thr Thr 1010 1015 1020 Ala Ala Gly Thr Gly Thr Ala Ala Gly Thr Gly Thr Gly Gly Thr Ala 1025 1030 1035 1040 Gly Thr Thr Ala Thr Cys Ala Thr Thr Gly Gly Ala Cys Thr Gly Thr 1045 1050 1055 Thr Gly Gly Thr Gly Ala Thr Thr Gly Gly Ala Cys Thr Thr Cys Cys 1060 1065 1070 Thr Ala Thr Gly Thr Cys Thr Cys Cys Ala Cys Thr Thr Gly Cys Thr 1075 1080 1085 Gly Thr Gly Gly Cys Thr Thr Thr Ala Ala Gly Thr Gly Thr Ala Ala 1090 1095 1100 Gly Cys Cys Ala Gly Thr Cys Cys Thr Thr Gly Thr Gly Gly Cys Thr 1105 1110 1115 1120 Thr Cys Ala Thr Gly Cys Thr Cys Thr Gly Cys Thr Ala Cys Gly Cys 1125 1130 1135 Cys Thr Gly Gly Thr Thr Cys Thr Gly Thr Thr Gly Thr Gly Gly Thr 1140 1145 1150 Thr Ala Cys Gly Cys Gly Cys Gly Cys Thr Gly Gly Thr Gly Cys Thr 1155 1160 1165 Gly Gly Cys Ala Cys Thr Gly Gly Thr Gly Thr Thr Ala Ala Gly Thr 1170 1175 1180 Ala Thr Thr Ala Cys Ala Ala Cys Ala Ala Cys Ala Thr Gly Thr Thr 1185 1190 1195 1200 Cys Cys Thr Gly Cys Gly Cys Cys Ala Thr Gly Thr Gly Gly Cys Ala 1205 1210 1215 Gly Ala Cys Ala Thr Thr Gly Ala Thr Gly Gly Gly Thr Thr Gly Gly 1220 1225 1230 Cys Ala Thr Thr Cys Thr Gly Gly Cys Gly Ala Ala Thr Thr Cys Thr 1235 1240 1245 Cys Ala Ala Gly Gly Thr Gly Cys Ala Gly Thr Cys Cys Ala Ala Ala 1250 1255 1260 Gly Ala Cys Gly Ala Cys Cys Thr Cys Gly Cys Thr Thr Gly Cys Thr 1265 1270 1275 1280 Cys Thr Gly Gly Thr Ala Ala Ala Thr Thr Cys Cys Thr Thr Gly Ala 1285 1290 1295 Ala Cys Ala Cys Cys Ala Thr Gly Ala Gly Gly Ala Ala Gly Gly Thr 1300 1305 1310 Thr Thr Cys Ala Cys Ala Gly Ala Thr Cys Cys Thr Thr Gly Cys Thr 1315 1320 1325 Ala Cys Thr Thr Thr Thr Thr Gly Ala Ala Thr Gly Ala Cys Thr Cys 1330 1335 1340 Gly Ala Gly Cys Ala Thr Thr Gly Cys Thr Ala Cys Thr Ala Ala Gly 1345 1350 1355 1360 Cys Thr Cys Ala Ala Gly Thr Thr Thr Gly Ala Cys Ala Thr Cys Cys 1365 1370 1375 Thr Thr Ala Gly Thr Gly Gly Cys Ala Ala Gly Thr Thr Thr Thr Cys 1380 1385 1390 Thr Gly Ala Thr Gly Ala Ala Gly Thr Cys Ala Ala Ala Cys Ala Ala 1395 1400 1405 Gly Cys Thr Ala Thr Cys Thr Thr Thr Gly Cys Thr Gly Gly Thr Cys 1410 1415 1420 Ala Thr Gly Thr Thr Gly Thr Thr Gly Thr Thr Gly Gly Cys Ala Gly 1425 1430 1435 1440 Cys Gly Cys Gly Cys Thr Cys Gly Thr Thr Gly Ala Cys Ala Thr Thr 1445 1450 1455 Gly Thr Thr Gly Ala Cys Gly Ala Thr Gly Cys Ala Cys Thr Gly Gly 1460 1465 1470 Gly Ala Cys Ala Gly Cys Cys Thr Thr Gly Gly Thr Thr Thr Ala Thr 1475 1480 1485 Ala 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27745 27750 27755 27760 Ala Thr Thr Ala Cys Thr Gly Ala Gly Thr Gly Thr Thr Thr Thr Thr 27765 27770 27775 Cys Thr Ala Gly Cys Gly Ala Cys Thr Thr Gly Gly Cys Thr Gly Cys 27780 27785 27790 Thr Gly Gly Gly Cys Thr Ala Thr Gly Gly Cys Thr Thr Thr Gly Cys 27795 27800 27805 Cys Cys Thr Cys Thr Ala Ala Cys Thr Ala Gly Cys Gly Gly Thr Cys 27810 27815 27820 Thr Thr Gly Gly Thr Cys Thr Thr Gly Cys Ala Cys Ala Cys Ala Ala 27825 27830 27835 27840 Cys Gly Gly Thr Ala Ala Gly Cys Cys Ala Gly Thr Gly Gly Thr Ala 27845 27850 27855 Ala Thr Gly Thr Cys Ala Gly Thr Gly Cys Ala Ala Gly Ala Ala Gly 27860 27865 27870 Gly Ala Thr Ala Thr Thr Ala Cys Cys Ala Thr Ala Gly Cys Ala Cys 27875 27880 27885 Thr Gly Thr Cys Ala Thr Gly Ala Gly Gly Gly Gly Ala Ala Cys Gly 27890 27895 27900 Cys Ala Gly Thr Ala Cys Cys Thr Thr Thr Thr Cys Ala Thr Cys Thr 27905 27910 27915 27920 Ala Ala Ala Cys Cys Thr Thr Thr Gly Cys Ala Cys Gly Ala Gly Thr 27925 27930 27935 Ala Ala Thr Cys Ala Ala Ala Gly Ala Thr Cys Cys Gly Cys Thr Thr 27940 27945 27950 Gly Ala Cys Gly Ala Gly Cys Cys Thr Ala Thr Ala Thr Gly Gly Ala 27955 27960 27965 Ala Gly Ala Gly Cys Gly Thr Gly Cys Cys Ala Gly Gly Thr Ala Thr 27970 27975 27980 Thr Thr Gly Ala Cys Thr Cys Ala Ala Gly Gly Ala Cys Thr Gly Thr 27985 27990 27995 28000 Thr Ala Gly Thr Ala Ala Cys Thr Gly Ala Ala Gly Ala Cys Cys Thr 28005 28010 28015 Gly Ala Cys Gly Gly Thr Gly Thr Thr Gly Ala Thr Ala Thr Gly Gly 28020 28025 28030 Ala Thr Ala Cys Ala Cys 28035 <210> 22 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 spike protein <400> 22 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 23 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 ORF3 <400> 23 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Asp Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 24 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 envelope protein <400> 24 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Pro Ser Thr Val Ile Asp Val 65 70 75 <210> 25 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 membrane protein <400> 25 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 26 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 nucleocapsid protein <400> 26 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 27 <211> 7405 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P3 complete cds <400> 27 Ala Thr Gly Ala Ala Gly Thr Cys Thr Thr Thr Ala Ala Cys Cys Thr 1 5 10 15 Ala Cys Thr Thr Cys Thr Gly Gly Thr Thr Gly Thr Thr Cys Thr Thr 20 25 30 Ala Cys Cys Ala Gly Thr Ala Cys Thr Thr Thr Cys Ala Ala Cys Ala 35 40 45 Cys Thr Thr Ala Gly Cys Cys Thr Ala Cys Cys Ala Cys Ala Ala Gly 50 55 60 Ala Thr Gly Thr Cys Ala Cys Cys Ala Gly Gly Thr Gly Cys Thr Cys 65 70 75 80 Ala Gly Cys Thr Ala Ala Cys Ala Cys Thr Ala Ala Thr Thr Thr Thr 85 90 95 Ala Gly Gly Cys Gly Gly Thr Thr Cys Thr Thr Thr Thr Cys Ala Ala 100 105 110 Ala Ala Thr Thr Thr Ala Ala Thr Gly Thr Thr Cys Ala Gly Gly Cys 115 120 125 Gly Cys Cys Thr Gly Cys Ala Gly Thr Thr Gly Thr Thr Gly Thr Ala 130 135 140 Cys Thr Gly Gly Gly Cys Gly Gly Thr Thr Ala Thr Cys Thr Ala Cys 145 150 155 160 Cys Thr Ala Thr Thr Gly Gly Thr Gly Ala Ala Ala Ala Cys Cys Ala 165 170 175 Gly Gly Gly Thr Gly Thr Cys Ala Ala Thr Thr Cys Ala Ala Cys Thr 180 185 190 Thr Gly Gly Thr Ala Cys Thr Gly Thr Gly Cys Thr Gly Gly Cys Cys 195 200 205 Ala Ala Cys Ala Thr Cys Cys Ala Ala Cys Thr Gly Cys Thr Ala Gly 210 215 220 Thr Gly Gly Cys Gly Thr Thr Cys Ala Thr Gly Gly Thr Ala Thr Cys 225 230 235 240 Thr Thr Thr Gly Thr Thr Ala Gly Cys Cys Ala Thr Ala Thr Thr Ala 245 250 255 Gly Ala Gly Gly Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Thr Thr 260 265 270 Thr Gly Ala Gly Ala Thr Thr Gly Gly Cys Ala Thr Thr Thr Cys Gly 275 280 285 Cys Ala Ala Gly Ala Gly Cys Cys Thr Thr Thr Thr Gly Ala Cys Cys 290 295 300 Cys Thr Ala Gly Thr Gly Gly Thr Thr Ala Cys Cys Ala Gly Cys Thr 305 310 315 320 Thr Thr Ala Thr Thr Thr Ala Cys Ala Thr Ala Ala Gly Gly Cys Thr 325 330 335 Ala Cys Thr Ala Ala Cys Gly Gly Thr Ala Ala Cys Ala Cys Thr Ala 340 345 350 Ala Thr Gly Cys Thr Ala Cys Thr Gly Cys Gly Cys Gly Ala Cys Thr 355 360 365 Gly Cys Gly Cys Ala Thr Thr Thr Gly Cys Cys Ala Gly Thr Thr Thr 370 375 380 Cys Cys Thr Ala Gly Cys Ala Thr Thr Ala Ala Ala Ala Cys Ala Thr 385 390 395 400 Thr Gly Gly Gly Cys Cys Cys Cys Ala Cys Thr Gly Cys Thr Ala Ala 405 410 415 Thr Ala Ala Thr Gly Ala Thr Gly Thr Thr Ala Cys Ala Ala Cys Ala 420 425 430 Gly Gly Thr Cys Gly Thr Ala Ala Thr Thr Gly Cys Cys Thr Ala Thr 435 440 445 Thr Thr Ala Ala Cys Ala Ala Ala Gly Cys Cys Ala Thr Cys Cys Cys 450 455 460 Ala Gly Cys Thr Cys Ala Thr Ala Thr Gly Ala Gly Thr Gly Ala Ala 465 470 475 480 Cys Ala Thr Ala Gly Thr Gly Thr Thr Gly Thr Cys Gly Gly Cys Ala 485 490 495 Thr Ala Ala Cys Ala Thr Gly Gly Gly Ala Thr Ala Ala Thr Gly Ala 500 505 510 Thr Cys Gly Thr Gly Thr Cys Ala Cys Thr Gly Thr Cys Thr Thr Thr 515 520 525 Thr Cys Thr Gly Ala Cys Ala Ala Gly Ala Thr Cys Thr Ala Thr Thr 530 535 540 Ala Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Ala Ala Ala Ala Ala 545 550 555 560 Thr Gly Ala Thr Thr Gly Gly Thr Cys Cys Cys Gly Thr Gly Thr Thr 565 570 575 Gly Cys Gly Ala Cys Ala Ala Ala Gly Thr Gly Thr Thr Ala Cys Ala 580 585 590 Ala Cys Ala Gly Thr Gly Gly Ala Gly Gly Thr Thr Gly Thr Gly Cys 595 600 605 Thr Ala Thr Gly Cys Ala Ala Thr Ala Thr Gly Thr Thr Thr Ala Cys 610 615 620 Gly Ala Ala Cys Cys Cys Ala Cys Cys Thr Ala Thr Thr Ala Cys Ala 625 630 635 640 Thr Gly Cys Thr Thr Ala Ala Thr Gly Thr Thr Ala Cys Thr Ala Gly 645 650 655 Thr Gly Cys Thr Gly Gly Thr Gly Ala Gly Gly Ala Thr Gly Gly Thr 660 665 670 Ala Thr Thr Thr Cys Thr Thr Ala Thr Cys Ala Ala Cys Cys Cys Thr 675 680 685 Gly Thr Ala Cys Ala Gly Cys Thr Ala Ala Thr Thr Gly Cys Ala Thr 690 695 700 Thr Gly Gly Thr Thr Ala Thr Gly Cys Thr Gly Cys Cys Ala Ala Thr 705 710 715 720 Gly Thr Ala Thr Thr Thr Gly Cys Thr Ala Cys Thr Gly Ala Gly Cys 725 730 735 Cys Cys Ala Ala Thr Gly Gly Cys Cys Ala Cys Ala Thr Ala Cys Cys 740 745 750 Ala Gly Ala Ala Gly Gly Thr Thr Thr Thr Ala Gly Thr Thr Thr Thr 755 760 765 Ala Ala Thr Ala Ala Thr Thr Gly Gly Thr Thr Thr Cys Thr Thr Thr 770 775 780 Thr Gly Thr Cys Cys Ala Ala Thr Gly Ala Thr Thr Cys Cys Ala Cys 785 790 795 800 Thr Thr Thr Gly Gly Thr Gly Cys Ala Thr Gly Gly Thr Ala Ala Gly 805 810 815 Gly Thr Gly Gly Thr Thr Thr Cys Cys Ala Ala Cys Cys Ala Ala Cys 820 825 830 Cys Ala Thr Thr Gly Thr Thr Gly Gly Thr Cys Ala Ala Thr Thr Gly 835 840 845 Thr Cys Thr Thr Thr Thr Gly Gly Cys Cys Ala Thr Thr Cys Cys Thr 850 855 860 Ala Ala Gly Ala Thr Thr Thr Ala Thr Gly Gly Ala Cys Thr Ala Gly 865 870 875 880 Gly Cys Cys Ala Ala Thr Thr Thr Thr Thr Cys Thr Cys Cys Thr Thr 885 890 895 Thr Ala Ala Thr Cys Ala Ala Ala Cys Gly Ala Thr Cys Gly Ala Thr 900 905 910 Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Ala Gly 915 920 925 Cys Thr Gly Cys Thr Gly Thr Gly Cys Ala Gly Cys Gly Thr Gly Cys 930 935 940 Ala Cys Cys Ala Gly Ala Gly Gly Cys Thr Cys Thr Gly Ala Gly Gly 945 950 955 960 Thr Thr Thr Ala Ala Thr Ala Thr Thr Ala Ala Thr Gly Ala Cys Ala 965 970 975 Cys Cys Thr Cys Thr Gly Thr Cys Ala Thr Thr Cys Thr Thr Gly Cys 980 985 990 Thr Gly Ala Ala Gly Gly Cys Thr Cys Ala Ala Thr Thr Gly Thr Ala 995 1000 1005 Cys Thr Thr Cys Ala Thr Ala Cys Thr Gly Cys Thr Thr Thr Ala Gly 1010 1015 1020 Gly Ala Ala Cys Ala Ala Ala Thr Thr Thr Thr Thr Cys Thr Thr Thr 1025 1030 1035 1040 Thr Gly Thr Thr Thr Gly Cys Ala Gly Thr Ala Ala Thr Thr Cys Cys 1045 1050 1055 Thr Cys Ala Ala Ala Thr Cys Cys Thr Cys Ala Cys Thr Thr Ala Gly 1060 1065 1070 Cys Cys Ala Cys Cys Thr Thr Cys Gly Cys Cys Ala Thr Ala Cys Cys 1075 1080 1085 Thr Thr Thr Gly Gly Gly Thr Gly Cys Thr Ala Cys Cys Cys Ala Ala 1090 1095 1100 Gly Thr Ala Cys Cys Cys Thr Ala Thr Thr Ala Thr Thr Gly Thr Thr 1105 1110 1115 1120 Thr Thr Thr Thr Thr Ala Ala Ala Gly Thr Gly Gly Ala Thr Ala Cys 1125 1130 1135 Thr Thr Ala Cys Ala Ala Cys Thr Cys Cys Ala Cys Thr Gly Thr Thr 1140 1145 1150 Thr Ala Thr Ala Ala Ala Thr Thr Cys Thr Thr Gly Gly Cys Thr Gly 1155 1160 1165 Thr Thr Thr Thr Ala Cys Cys Thr Cys Cys Ala Ala Cys Cys Gly Thr 1170 1175 1180 Cys Ala Gly Gly Gly Ala Ala Ala Thr Thr Gly Thr Cys Ala Thr Cys 1185 1190 1195 1200 Ala Cys Cys Ala Ala Gly Thr Ala Thr Gly Gly Thr Gly Ala Thr Gly 1205 1210 1215 Thr Thr Thr Ala Thr Gly Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1220 1225 1230 Thr Gly Gly Ala Thr Ala Cys Thr Thr Gly Cys Ala Thr Cys Thr Cys 1235 1240 1245 Gly Gly Thr Thr Thr Gly Thr Thr Gly Gly Ala Thr Gly Cys Thr Gly 1250 1255 1260 Thr Cys Ala Cys Ala Ala Thr Thr Ala Ala Thr Thr Thr Cys Ala Cys 1265 1270 1275 1280 Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Ala Cys Thr Gly Ala Cys 1285 1290 1295 Gly Ala Thr Gly Ala Thr Gly Thr Thr Thr Cys Thr Gly Gly Thr Thr 1300 1305 1310 Thr Thr Thr Gly Gly Ala Cys Cys Ala Thr Ala Gly Cys Ala Thr Cys 1315 1320 1325 Gly Ala Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Thr Gly Ala Thr 1330 1335 1340 Gly Cys Ala Cys Thr Cys Ala Thr Cys Gly Ala Ala Gly Thr Thr Cys 1345 1350 1355 1360 Ala Ala Gly Gly Ala Ala Cys Cys Gly Cys Cys Ala Thr Thr Cys Ala 1365 1370 1375 Gly Cys Gly Thr Ala Thr Thr Cys Thr Thr Thr Ala Thr Thr Gly Thr 1380 1385 1390 Gly Ala Thr Gly Ala Thr Cys Cys Thr Gly Thr Thr Ala Gly Cys Cys 1395 1400 1405 Ala Ala Cys Thr Cys Ala Ala Gly Thr Gly Thr Thr Cys Thr Cys Ala 1410 1415 1420 Gly Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Ala Cys Cys Thr Thr 1425 1430 1435 1440 Gly Ala Cys Gly Ala Thr Gly Gly Thr Thr Thr Thr Thr Ala Cys Cys 1445 1450 1455 Cys Thr Ala Thr Thr Thr Cys Thr Thr Cys Thr Ala Gly Ala Ala Ala 1460 1465 1470 Cys Cys Thr Thr Cys Thr Gly Ala Gly Thr Cys Ala Thr Gly Ala Ala 1475 1480 1485 Cys Ala Gly Cys Cys Ala Ala Thr Thr Thr Cys Thr Thr Thr Thr Gly 1490 1495 1500 Thr Thr Ala Cys Thr Cys Thr Gly Cys Cys Ala Thr Cys Ala Thr Thr 1505 1510 1515 1520 Thr Ala Ala Thr Gly Ala Thr Cys Ala Thr Thr Cys Thr Thr Thr Thr 1525 1530 1535 Gly Thr Thr Ala Ala Cys Ala Thr Thr Ala Cys Thr Gly Thr Ala Thr 1540 1545 1550 Cys Thr Gly Cys Thr Thr Cys Cys Thr Thr Thr Gly Gly Thr Gly Gly 1555 1560 1565 Thr Cys Ala Thr Ala Gly Thr Gly Gly Thr Gly Cys Cys Ala Ala Cys 1570 1575 1580 Cys Thr Thr Ala Thr Thr Gly Cys Ala Thr Cys Thr Gly Ala Cys Ala 1585 1590 1595 1600 Cys Thr Ala Cys Thr Ala Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1605 1610 1615 Thr Ala Gly Thr Thr Cys Thr Thr Thr Cys Thr Gly Thr Gly Thr Thr 1620 1625 1630 Gly Ala Cys Ala Cys Thr Ala Gly Ala Cys Ala Ala Thr Thr Thr Ala 1635 1640 1645 Cys Cys Ala Thr Thr Thr Cys Ala Cys Thr Gly Thr Thr Thr Thr Ala 1650 1655 1660 Thr Ala Ala Cys Gly Thr Thr Ala Cys Ala Ala Ala Cys Ala Gly Thr 1665 1670 1675 1680 Thr Ala Thr Gly Gly Thr Thr Ala Thr Gly Thr Gly Thr Cys Thr Ala 1685 1690 1695 Ala Ala Thr Cys Ala Cys Ala Gly Gly Ala Cys Ala Gly Thr Ala Ala 1700 1705 1710 Thr Thr Gly Cys Cys Cys Thr Thr Thr Cys Ala Cys Cys Thr Thr Gly 1715 1720 1725 Cys Ala Ala Thr Cys Thr Gly Thr Thr Ala Ala Thr Gly Ala Thr Thr 1730 1735 1740 Ala Cys Cys Thr Gly Thr Cys Thr Thr Thr Thr Ala Gly Cys Ala Ala 1745 1750 1755 1760 Ala Thr Thr Thr Thr Gly Thr Gly Thr Thr Thr Cys Cys Ala Cys Cys 1765 1770 1775 Ala Gly Cys Cys Thr Thr Thr Thr Gly Gly Cys Thr Ala Gly Thr Gly 1780 1785 1790 Cys Cys Thr Gly Thr Ala Cys Cys Ala Thr Ala Gly Ala Thr Cys Thr 1795 1800 1805 Thr Thr Thr Thr Gly Gly Thr Thr Ala Cys Cys Cys Thr Gly Ala Gly 1810 1815 1820 Thr Thr Thr Gly Gly Thr Ala Gly Thr Gly Gly Thr Gly Thr Thr Ala 1825 1830 1835 1840 Ala Gly Thr Thr Thr Ala Cys Gly Thr Cys Cys Cys Thr Thr Thr Ala 1845 1850 1855 Cys Thr Thr Thr Cys Ala Ala Thr Thr Cys Ala Cys Ala Ala Ala Gly 1860 1865 1870 Gly Gly Thr Gly Ala Gly Thr Thr Gly Ala Thr Thr Ala Cys Thr Gly 1875 1880 1885 Gly Cys Ala Cys Gly Cys Cys Thr Ala Ala Ala Cys Cys Ala Cys Thr 1890 1895 1900 Thr Gly Ala Ala Gly Gly Thr Gly Thr Cys Ala Cys Gly Gly Ala Cys 1905 1910 1915 1920 Gly Thr Thr Thr Cys Thr Thr Thr Thr Ala Thr Gly Ala Cys Thr Cys 1925 1930 1935 Thr Gly Gly Ala Thr Gly Thr Gly Thr Gly Thr Ala Cys Cys Ala Ala 1940 1945 1950 Gly Thr Ala Thr Ala Cys Thr Ala Thr Cys Thr Ala Thr Gly Gly Cys 1955 1960 1965 Thr Thr Thr Ala Ala Ala Gly Gly Thr Gly Ala Gly Gly Gly Thr Ala 1970 1975 1980 Thr Cys Cys Thr Thr Ala Cys Cys Cys Thr Thr Ala Cys Ala Ala Ala 1985 1990 1995 2000 Thr Thr Cys Thr Ala Gly Cys Thr Thr Thr Thr Thr Gly Gly Cys Ala 2005 2010 2015 Gly Gly Thr Gly Thr Thr Thr Ala Thr Thr Ala Cys Ala Cys Ala Thr 2020 2025 2030 Cys Thr Gly Ala Thr Thr Cys Thr Gly Gly Ala Cys Ala Gly Thr Thr 2035 2040 2045 Gly Thr Thr Ala Gly Cys Cys Thr Thr Thr Ala Ala Gly Ala Ala Thr 2050 2055 2060 Gly Thr Cys Ala Cys Thr Ala Gly Thr Gly Gly Thr Gly Cys Thr Gly 2065 2070 2075 2080 Thr Thr Thr Ala Thr Thr Cys Thr Gly Thr Thr Ala Cys Gly Cys Cys 2085 2090 2095 Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Cys Ala Gly Ala Gly 2100 2105 2110 Cys Ala Gly Gly Cys Thr Gly Cys Ala Thr Ala Thr Gly Thr Thr Gly 2115 2120 2125 Ala Thr Gly Ala Thr Gly Ala Thr Ala Thr Ala Gly Thr Gly Gly Gly 2130 2135 2140 Thr Gly Thr Thr Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Thr Gly 2145 2150 2155 2160 Thr Cys Thr Ala Gly Cys Thr Cys Cys Ala Cys Thr Thr Thr Thr Ala 2165 2170 2175 Ala Cys Ala Gly Thr Ala Cys Thr Ala Gly Gly Gly Ala Gly Thr Thr 2180 2185 2190 Gly Cys Cys Thr Gly Gly Thr Thr Thr Cys Thr Thr Cys Thr Ala Cys 2195 2200 2205 Cys Ala Thr Thr Cys Thr Ala Ala Thr Gly Ala Thr Gly Gly Cys Thr 2210 2215 2220 Cys Thr Ala Ala Thr Thr Gly Thr Ala Cys Ala Gly Ala Gly Cys Cys 2225 2230 2235 2240 Thr Gly Thr Gly Thr Thr Gly Gly Thr Gly Thr Ala Thr Ala Gly Thr 2245 2250 2255 Ala Ala Cys Ala Thr Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala 2260 2265 2270 Ala Ala Thr Cys Thr Gly Gly Cys Ala Gly Thr Ala Thr Thr Gly Gly 2275 2280 2285 Cys Thr Ala Cys Gly Thr Cys Cys Cys Ala Thr Cys Thr Cys Ala Gly 2290 2295 2300 Thr Cys Thr Gly Gly Cys Cys Ala Ala Gly Thr Cys Ala Ala Gly Ala 2305 2310 2315 2320 Thr Thr Gly Cys Ala Cys Cys Cys Ala Cys Gly Gly Thr Thr Ala Cys 2325 2330 2335 Thr Gly Gly Gly Ala Ala Thr Ala Thr Thr Ala Gly Thr Ala Thr Thr 2340 2345 2350 Cys Cys Cys Ala Cys Cys Ala Ala Cys Thr Thr Thr Ala Gly Thr Ala 2355 2360 2365 Thr Gly Ala Gly Thr Ala Thr Thr Ala Gly Gly Ala Cys Ala Gly Ala 2370 2375 2380 Ala Thr Ala Thr Thr Thr Ala Cys Ala Gly Cys Thr Thr Thr Ala Cys 2385 2390 2395 2400 Ala Ala Cys Ala Cys Gly Cys Cys Thr Gly Thr Thr Ala Gly Thr Gly 2405 2410 2415 Thr Thr Gly Ala Thr Thr Gly Thr Gly Cys Cys Ala Cys Ala Thr Ala 2420 2425 2430 Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Thr Ala Ala Cys 2435 2440 2445 Thr Cys Thr Cys Gly Thr Thr Gly Thr Ala Ala Ala Cys Ala Ala Thr 2450 2455 2460 Thr Ala Cys Thr Cys Ala Cys Cys Cys Ala Gly Thr Ala Cys Ala Cys 2465 2470 2475 2480 Thr Gly Cys Ala Gly Cys Ala Thr Gly Thr Ala Ala Gly Ala Cys Cys 2485 2490 2495 Ala Thr Ala Gly Ala Gly Thr Cys Ala Gly Cys Ala Thr Thr Ala Cys 2500 2505 2510 Ala Ala Cys Thr Cys Ala Gly Cys Gly Cys Thr Ala Gly Gly Cys Thr 2515 2520 2525 Thr Gly Ala Gly Thr Cys Thr Gly Thr Thr Gly Ala Ala Gly Thr Thr 2530 2535 2540 Ala Ala Cys Thr Cys Thr Ala Thr Gly Cys Thr Thr Ala Cys Thr Ala 2545 2550 2555 2560 Thr Thr Thr Cys Thr Gly Ala Ala Gly Ala Gly Gly Cys Thr Cys Thr 2565 2570 2575 Ala Cys Ala Gly Thr Thr Ala Gly Cys Thr Ala Cys Cys Ala Thr Thr 2580 2585 2590 Ala Gly Thr Thr Cys Gly Thr Thr Thr Ala Ala Thr Gly Gly Thr Gly 2595 2600 2605 Ala Thr Gly Gly Ala Thr Ala Thr Ala Ala Thr Thr Thr Thr Ala Cys 2610 2615 2620 Thr Ala Ala Thr Gly Thr Gly Cys Thr Gly Gly Gly Thr Gly Thr Thr 2625 2630 2635 2640 Thr Cys Thr Gly Thr Gly Thr Ala Thr Gly Ala Thr Cys Cys Thr Gly 2645 2650 2655 Cys Ala Ala Gly Thr Gly Gly Cys Ala Gly Gly Gly Thr Gly Gly Thr 2660 2665 2670 Ala Cys Ala Ala Ala Ala Ala Ala Gly Gly Thr Cys Thr Thr Thr Thr 2675 2680 2685 Ala Thr Thr Gly Ala Ala Gly Ala Cys Cys Thr Gly Cys Thr Thr Thr 2690 2695 2700 Thr Thr Ala Ala Thr Ala Ala Ala Gly Thr Gly Gly Thr Thr Ala Cys 2705 2710 2715 2720 Thr Ala Ala Thr Gly Gly Cys Cys Thr Thr Gly Gly Thr Ala Cys Thr 2725 2730 2735 Gly Thr Thr Gly Ala Thr Gly Ala Ala Gly Ala Cys Thr Ala Thr Ala 2740 2745 2750 Ala Gly Cys Gly Cys Thr Gly Thr Thr Cys Thr Ala Ala Thr Gly Gly 2755 2760 2765 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Cys Cys Ala Thr Cys Thr Gly 6965 6970 6975 Ala Thr Gly Thr Gly Ala Cys Thr Cys Ala Thr Gly Cys Cys Ala Ala 6980 6985 6990 Thr Thr Thr Gly Gly Ala Ala Thr Gly Gly Gly Ala Cys Ala Cys Ala 6995 7000 7005 Gly Cys Thr Gly Thr Thr Gly Ala Thr Gly Gly Thr Gly Gly Thr Gly 7010 7015 7020 Ala Cys Ala Cys Gly Gly Cys Cys Gly Thr Thr Gly Ala Ala Ala Thr 7025 7030 7035 7040 Thr Ala Thr Cys Ala Ala Cys Gly Ala Gly Ala Thr Cys Thr Thr Cys 7045 7050 7055 Gly Ala Cys Ala Cys Ala Gly Gly Ala Ala Ala Thr Thr Ala Ala Ala 7060 7065 7070 Cys Ala Ala Thr Gly Thr Thr Thr Gly Ala Cys Thr Gly Gly Cys Thr 7075 7080 7085 Thr Ala Thr Cys Cys Thr Gly Gly Cys Thr Ala Thr Gly Thr Cys Cys 7090 7095 7100 Cys Ala Gly Gly Gly Thr Ala Gly Thr Gly Cys Cys Ala Thr Thr Ala 7105 7110 7115 7120 Cys Ala Cys Thr Gly Thr Thr Ala Thr Thr Ala Cys Thr Gly Ala Gly 7125 7130 7135 Thr Gly Thr Thr Thr Thr Thr Cys Thr Ala Gly Cys Gly Ala Cys Thr 7140 7145 7150 Thr Gly Gly Cys Thr Gly Cys Thr Gly Gly Gly Cys Thr Ala Thr Gly 7155 7160 7165 Gly Cys Thr Thr Thr Gly Cys Cys Cys Thr Cys Thr Ala Ala Cys Thr 7170 7175 7180 Ala Gly Cys Gly Gly Thr Cys Thr Thr Gly Gly Thr Cys Thr Thr Gly 7185 7190 7195 7200 Cys Ala Cys Ala Cys Ala Ala Cys Gly Gly Thr Ala Ala Gly Cys Cys 7205 7210 7215 Ala Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Cys Ala Gly Thr Gly 7220 7225 7230 Cys Ala Ala Gly Ala Ala Gly Gly Ala Thr Ala Thr Thr Ala Cys Cys 7235 7240 7245 Ala Thr Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Thr Gly Ala Gly 7250 7255 7260 Gly Gly Gly Ala Ala Cys Gly Cys Ala Gly Thr Ala Cys Cys Thr Thr 7265 7270 7275 7280 Thr Thr Cys Ala Thr Cys Thr Ala Ala Ala Cys Cys Thr Thr Thr Gly 7285 7290 7295 Cys Ala Cys Gly Ala Gly Thr Ala Ala Thr Cys Ala Ala Ala Gly Ala 7300 7305 7310 Thr Cys Cys Gly Cys Thr Thr Gly Ala Cys Gly Ala Gly Cys Cys Thr 7315 7320 7325 Ala Thr Ala Thr Gly Gly Ala Ala Gly Ala Gly Cys Gly Thr Gly Cys 7330 7335 7340 Cys Ala Gly Gly Thr Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 7345 7350 7355 7360 Gly Gly Ala Cys Thr Gly Thr Thr Ala Gly Thr Ala Ala Cys Thr Gly 7365 7370 7375 Ala Ala Gly Ala Cys Cys Thr Gly Ala Cys Gly Gly Thr Gly Thr Thr 7380 7385 7390 Gly Ala Thr Ala Thr Gly Gly Ala Thr Ala Cys Ala Cys 7395 7400 7405 <210> 28 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 spike protein <400> 28 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Glu Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 29 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 ORF3 <400> 29 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Asp Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 30 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 envelope protein <400> 30 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Pro Ser Thr Val Ile Asp Val 65 70 75 <210> 31 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 membrane protein <400> 31 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 32 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 nucleocapsid protein <400> 32 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 33 <211> 7405 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P4 complete cds <400> 33 Ala Thr Gly Ala Ala Gly Thr Cys Thr Thr Thr Ala Ala Cys Cys Thr 1 5 10 15 Ala Cys Thr Thr Cys Thr Gly Gly Thr Thr Gly Thr Thr Cys Thr Thr 20 25 30 Ala Cys Cys Ala Gly Thr Ala Cys Thr Thr Thr Cys Ala Ala Cys Ala 35 40 45 Cys Thr Thr Ala Gly Cys Cys Thr Ala Cys Cys Ala Cys Ala Ala Gly 50 55 60 Ala Thr Gly Thr Cys Ala Cys Cys Ala Gly Gly Thr Gly Cys Thr Cys 65 70 75 80 Ala Gly Cys Thr Ala Ala Cys Ala Cys Thr Ala Ala Thr Thr Thr Thr 85 90 95 Ala Gly Gly Cys Gly Gly Thr Thr Cys Thr Thr Thr Thr Cys Ala Ala 100 105 110 Ala Ala Thr Thr Thr Ala Ala Thr Gly Thr Thr Cys Ala Gly Gly Cys 115 120 125 Gly Cys Cys Thr Gly Cys Ala Gly Thr Thr Gly Thr Thr Gly Thr Ala 130 135 140 Cys Thr Gly Gly Gly Cys Gly Gly Thr Thr Ala Thr Cys Thr Ala Cys 145 150 155 160 Cys Thr Ala Thr Thr Gly Gly Thr Gly Ala Ala Ala Ala Cys Cys Ala 165 170 175 Gly Gly Gly Thr Gly Thr Cys Ala Ala Thr Thr Cys Ala Ala Cys Thr 180 185 190 Thr Gly Gly Thr Ala Cys Thr Gly Thr Gly Cys Thr Gly Gly Cys Cys 195 200 205 Ala Ala Cys Ala Thr Cys Cys Ala Ala Cys Thr Gly Cys Thr Ala Gly 210 215 220 Thr Gly Gly Cys Gly Thr Thr Cys Ala Thr Gly Gly Thr Ala Thr Cys 225 230 235 240 Thr Thr Thr Gly Thr Thr Ala Gly Cys Cys Ala Thr Ala Thr Thr Ala 245 250 255 Gly Ala Gly Gly Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Thr Thr 260 265 270 Thr Gly Ala Gly Ala Thr Thr Gly Gly Cys Ala Thr Thr Thr Cys Gly 275 280 285 Cys Ala Ala Gly Ala Gly Cys Cys Thr Thr Thr Thr Gly Ala Cys Cys 290 295 300 Cys Thr Ala Gly Thr Gly Gly Thr Thr Ala Cys Cys Ala Gly Cys Thr 305 310 315 320 Thr Thr Ala Thr Thr Thr Ala Cys Ala Thr Ala Ala Gly Gly Cys Thr 325 330 335 Ala Cys Thr Ala Ala Cys Gly Gly Thr Ala Ala Cys Ala Cys Thr Ala 340 345 350 Ala Thr Gly Cys Thr Ala Cys Thr Gly Cys Gly Cys Gly Ala Cys Thr 355 360 365 Gly Cys Gly Cys Ala Thr Thr Thr Gly Cys Cys Ala Gly Thr Thr Thr 370 375 380 Cys Cys Thr Ala Gly Cys Ala Thr Thr Ala Ala Ala Ala Cys Ala Thr 385 390 395 400 Thr Gly Gly Gly Cys Cys Cys Cys Ala Cys Thr Gly Cys Thr Ala Ala 405 410 415 Thr Ala Ala Thr Gly Ala Thr Gly Thr Thr Ala Cys Ala Ala Cys Ala 420 425 430 Gly Gly Thr Cys Gly Thr Ala Ala Thr Thr Gly Cys Cys Thr Ala Thr 435 440 445 Thr Thr Ala Ala Cys Ala Ala Ala Gly Cys Cys Ala Thr Cys Cys Cys 450 455 460 Ala Gly Cys Thr Cys Ala Thr Ala Thr Gly Ala Gly Thr Gly Ala Ala 465 470 475 480 Cys Ala Thr Ala Gly Thr Gly Thr Thr Gly Thr Cys Gly Gly Cys Ala 485 490 495 Thr Ala Ala Cys Ala Thr Gly Gly Gly Ala Thr Ala Ala Thr Gly Ala 500 505 510 Thr Cys Gly Thr Gly Thr Cys Ala Cys Thr Gly Thr Cys Thr Thr Thr 515 520 525 Thr Cys Thr Gly Ala Cys Ala Ala Gly Ala Thr Cys Thr Ala Thr Thr 530 535 540 Ala Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Ala Ala Ala Ala Ala 545 550 555 560 Thr Gly Ala Thr Thr Gly Gly Thr Cys Cys Cys Gly Thr Gly Thr Thr 565 570 575 Gly Cys Gly Ala Cys Ala Ala Ala Gly Thr Gly Thr Thr Ala Cys Ala 580 585 590 Ala Cys Ala Gly Thr Gly Gly Ala Gly Gly Thr Thr Gly Thr Gly Cys 595 600 605 Thr Ala Thr Gly Cys Ala Ala Thr Ala Thr Gly Thr Thr Thr Ala Cys 610 615 620 Gly Ala Ala Cys Cys Cys Ala Cys Cys Thr Ala Thr Thr Ala Cys Ala 625 630 635 640 Thr Gly Cys Thr Thr Ala Ala Thr Gly Thr Thr Ala Cys Thr Ala Gly 645 650 655 Thr Gly Cys Thr Gly Gly Thr Gly Ala Gly Gly Ala Thr Gly Gly Thr 660 665 670 Ala Thr Thr Thr Cys Thr Thr Ala Thr Cys Ala Ala Cys Cys Cys Thr 675 680 685 Gly Thr Ala Cys Ala Gly Cys Thr Ala Ala Thr Thr Gly Cys Ala Thr 690 695 700 Thr Gly Gly Thr Thr Ala Thr Gly Cys Thr Gly Cys Cys Ala Ala Thr 705 710 715 720 Gly Thr Ala Thr Thr Thr Gly Cys Thr Ala Cys Thr Gly Ala Gly Cys 725 730 735 Cys Cys Ala Ala Thr Gly Gly Cys Cys Ala Cys Ala Thr Ala Cys Cys 740 745 750 Ala Gly Ala Ala Gly Gly Thr Thr Thr Thr Ala Gly Thr Thr Thr Thr 755 760 765 Ala Ala Thr Ala Ala Thr Thr Gly Gly Thr Thr Thr Cys Thr Thr Thr 770 775 780 Thr Gly Thr Cys Cys Ala Ala Thr Gly Ala Thr Thr Cys Cys Ala Cys 785 790 795 800 Thr Thr Thr Gly Gly Thr Gly Cys Ala Thr Gly Gly Thr Ala Ala Gly 805 810 815 Gly Thr Gly Gly Thr Thr Thr Cys Cys Ala Ala Cys Cys Ala Ala Cys 820 825 830 Cys Ala Thr Thr Gly Thr Thr Gly Gly Thr Cys Ala Ala Thr Thr Gly 835 840 845 Thr Cys Thr Thr Thr Thr Gly Gly Cys Cys Ala Thr Thr Cys Cys Thr 850 855 860 Ala Ala Gly Ala Thr Thr Thr Ala Thr Gly Gly Ala Cys Thr Ala Gly 865 870 875 880 Gly Cys Cys Ala Ala Thr Thr Thr Thr Thr Cys Thr Cys Cys Thr Thr 885 890 895 Thr Ala Ala Thr Cys Ala Ala Ala Cys Gly Ala Thr Cys Gly Ala Thr 900 905 910 Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Ala Gly 915 920 925 Cys Thr Gly Cys Thr Gly Thr Gly Cys Ala Gly Cys Gly Thr Gly Cys 930 935 940 Ala Cys Cys Ala Gly Ala Gly Gly Cys Thr Cys Thr Gly Ala Gly Gly 945 950 955 960 Thr Thr Thr Ala Ala Thr Ala Thr Thr Ala Ala Thr Gly Ala Cys Ala 965 970 975 Cys Cys Thr Cys Thr Gly Thr Cys Ala Thr Thr Cys Thr Thr Gly Cys 980 985 990 Thr Gly Ala Ala Gly Gly Cys Thr Cys Ala Ala Thr Thr Gly Thr Ala 995 1000 1005 Cys Thr Thr Cys Ala Thr Ala Cys Thr Gly Cys Thr Thr Thr Ala Gly 1010 1015 1020 Gly Ala Ala Cys Ala Ala Ala Thr Thr Thr Thr Thr Cys Thr Thr Thr 1025 1030 1035 1040 Thr Gly Thr Thr Thr Gly Cys Ala Gly Thr Ala Ala Thr Thr Cys Cys 1045 1050 1055 Thr Cys Ala Ala Ala Thr Cys Cys Thr Cys Ala Cys Thr Thr Ala Gly 1060 1065 1070 Cys Cys Ala Cys Cys Thr Thr Cys Gly Cys Cys Ala Thr Ala Cys Cys 1075 1080 1085 Thr Thr Thr Gly Gly Gly Thr Gly Cys Thr Ala Cys Cys Cys Ala Ala 1090 1095 1100 Gly Thr Ala Cys Cys Cys Thr Ala Thr Thr Ala Thr Thr Gly Thr Thr 1105 1110 1115 1120 Thr Thr Thr Thr Thr Ala Ala Ala Gly Thr Gly Gly Ala Thr Ala Cys 1125 1130 1135 Thr Thr Ala Cys Ala Ala Cys Thr Cys Cys Ala Cys Thr Gly Thr Thr 1140 1145 1150 Thr Ala Thr Ala Ala Ala Thr Thr Cys Thr Thr Gly Gly Cys Thr Gly 1155 1160 1165 Thr Thr Thr Thr Ala Cys Cys Thr Cys Cys Ala Ala Cys Cys Gly Thr 1170 1175 1180 Cys Ala Gly Gly Gly Ala Ala Ala Thr Thr Gly Thr Cys Ala Thr Cys 1185 1190 1195 1200 Ala Cys Cys Ala Ala Gly Thr Ala Thr Gly Gly Thr Gly Ala Thr Gly 1205 1210 1215 Thr Thr Thr Ala Thr Gly Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1220 1225 1230 Thr Gly Gly Ala Thr Ala Cys Thr Thr Gly Cys Ala Thr Cys Thr Cys 1235 1240 1245 Gly Gly Thr Thr Thr Gly Thr Thr Gly Gly Ala Thr Gly Cys Thr Gly 1250 1255 1260 Thr Cys Ala Cys Ala Ala Thr Thr Ala Ala Thr Thr Thr Cys Ala Cys 1265 1270 1275 1280 Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Ala Cys Thr Gly Ala Cys 1285 1290 1295 Gly Ala Thr Gly Ala Thr Gly Thr Thr Thr Cys Thr Gly Gly Thr Thr 1300 1305 1310 Thr Thr Thr Gly Gly Ala Cys Cys Ala Thr Ala Gly Cys Ala Thr Cys 1315 1320 1325 Gly Ala Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Thr Gly Ala Thr 1330 1335 1340 Gly Cys Ala Cys Thr Cys Ala Thr Cys Gly Ala Ala Gly Thr Thr Cys 1345 1350 1355 1360 Ala Ala Gly Gly Ala Ala Cys Cys Gly Cys Cys Ala Thr Thr Cys Ala 1365 1370 1375 Gly Cys Gly Thr Ala Thr Thr Cys Thr Thr Thr Ala Thr Thr Gly Thr 1380 1385 1390 Gly Ala Thr Gly Ala Thr Cys Cys Thr Gly Thr Thr Ala Gly Cys Cys 1395 1400 1405 Ala Ala Cys Thr Cys Ala Ala Gly Thr Gly Thr Thr Cys Thr Cys Ala 1410 1415 1420 Gly Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Ala Cys Cys Thr Thr 1425 1430 1435 1440 Gly Ala Cys Gly Ala Thr Gly Gly Thr Thr Thr Thr Thr Ala Cys Cys 1445 1450 1455 Cys Thr Ala Thr Thr Thr Cys Thr Thr Cys Thr Ala Gly Ala Ala Ala 1460 1465 1470 Cys Cys Thr Thr Cys Thr Gly Ala Gly Thr Cys Ala Thr Gly Ala Ala 1475 1480 1485 Cys Ala Gly Cys Cys Ala Ala Thr Thr Thr Cys Thr Thr Thr Thr Gly 1490 1495 1500 Thr Thr Ala Cys Thr Cys Thr Gly Cys Cys Ala Thr Cys Ala Thr Thr 1505 1510 1515 1520 Thr Ala Ala Thr Gly Ala Thr Cys Ala Thr Thr Cys Thr Thr Thr Thr 1525 1530 1535 Gly Thr Thr Ala Ala Cys Ala Thr Thr Ala Cys Thr Gly Thr Ala Thr 1540 1545 1550 Cys Thr Gly Cys Thr Thr Cys Cys Thr Thr Thr Gly Gly Thr Gly Gly 1555 1560 1565 Thr Cys Ala Thr Ala Gly Thr Gly Gly Thr Gly Cys Cys Ala Ala Cys 1570 1575 1580 Cys Thr Thr Ala Thr Thr Gly Cys Ala Thr Cys Thr Gly Ala Cys Ala 1585 1590 1595 1600 Cys Thr Ala Cys Thr Ala Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1605 1610 1615 Thr Ala Gly Thr Thr Cys Thr Thr Thr Cys Thr Gly Thr Gly Thr Thr 1620 1625 1630 Gly Ala Cys Ala Cys Thr Ala Gly Ala Cys Ala Ala Thr Thr Thr Ala 1635 1640 1645 Cys Cys Ala Thr Thr Thr Cys Ala Cys Thr Gly Thr Thr Thr Thr Ala 1650 1655 1660 Thr Ala Ala Cys Gly Thr Thr Ala Cys Ala Ala Ala Cys Ala Gly Thr 1665 1670 1675 1680 Thr Ala Thr Gly Gly Thr Thr Ala Thr Gly Thr Gly Thr Cys Thr Ala 1685 1690 1695 Ala Ala Thr Cys Ala Cys Ala Gly Gly Ala Cys Ala Gly Thr Ala Ala 1700 1705 1710 Thr Thr Gly Cys Cys Cys Thr Thr Thr Cys Ala Cys Cys Thr Thr Gly 1715 1720 1725 Cys Ala Ala Thr Cys Thr Gly Thr Thr Ala Ala Thr Gly Ala Thr Thr 1730 1735 1740 Ala Cys Cys Thr Gly Thr Cys Thr Thr Thr Thr Ala Gly Cys Ala Ala 1745 1750 1755 1760 Ala Thr Thr Thr Thr Gly Thr Gly Thr Thr Thr Cys Cys Ala Cys Cys 1765 1770 1775 Ala Gly Cys Cys Thr Thr Thr Thr Gly Gly Cys Thr Ala Gly Thr Gly 1780 1785 1790 Cys Cys Thr Gly Thr Ala Cys Cys Ala Thr Ala Gly Ala Thr Cys Thr 1795 1800 1805 Thr Thr Thr Thr Gly Gly Thr Thr Ala Cys Cys Cys Thr Gly Ala Gly 1810 1815 1820 Thr Thr Thr Gly Gly Thr Ala Gly Thr Gly Gly Thr Gly Thr Thr Ala 1825 1830 1835 1840 Ala Gly Thr Thr Thr Ala Cys Gly Thr Cys Cys Cys Thr Thr Thr Ala 1845 1850 1855 Cys Thr Thr Thr Cys Ala Ala Thr Thr Cys Ala Cys Ala Ala Ala Gly 1860 1865 1870 Gly Gly Thr Gly Ala Gly Thr Thr Gly Ala Thr Thr Ala Cys Thr Gly 1875 1880 1885 Gly Cys Ala Cys Gly Cys Cys Thr Ala Ala Ala Cys Cys Ala Cys Thr 1890 1895 1900 Thr Gly Ala Ala Gly Gly Thr Gly Thr Cys Ala Cys Gly Gly Ala Cys 1905 1910 1915 1920 Gly Thr Thr Thr Cys Thr Thr Thr Thr Ala Thr Gly Ala Cys Thr Cys 1925 1930 1935 Thr Gly Gly Ala Thr Gly Thr Gly Thr Gly Thr Ala Cys Cys Ala Ala 1940 1945 1950 Gly Thr Ala Thr Ala Cys Thr Ala Thr Cys Thr Ala Thr Gly Gly Cys 1955 1960 1965 Thr Thr Thr Ala Ala Ala Gly Gly Thr Gly Ala Gly Gly Gly Thr Ala 1970 1975 1980 Thr Cys Cys Thr Thr Ala Cys Cys Cys Thr Thr Ala Cys Ala Ala Ala 1985 1990 1995 2000 Thr Thr Cys Thr Ala Gly Cys Thr Thr Thr Thr Thr Gly Gly Cys Ala 2005 2010 2015 Gly Gly Thr Gly Thr Thr Thr Ala Thr Thr Ala Cys Ala Cys Ala Thr 2020 2025 2030 Cys Thr Gly Ala Thr Thr Cys Thr Gly Gly Ala Cys Ala Gly Thr Thr 2035 2040 2045 Gly Thr Thr Ala Gly Cys Cys Thr Thr Thr Ala Ala Gly Ala Ala Thr 2050 2055 2060 Gly Thr Cys Ala Cys Thr Ala Gly Thr Gly Gly Thr Gly Cys Thr Gly 2065 2070 2075 2080 Thr Thr Thr Ala Thr Thr Cys Thr Gly Thr Thr Ala Cys Gly Cys Cys 2085 2090 2095 Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Cys Ala Gly Ala Gly 2100 2105 2110 Cys Ala Gly Gly Cys Thr Gly Cys Ala Thr Ala Thr Gly Thr Thr Gly 2115 2120 2125 Ala Thr Gly Ala Thr Gly Ala Thr Ala Thr Ala Gly Thr Gly Gly Gly 2130 2135 2140 Thr Gly Thr Thr Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Thr Gly 2145 2150 2155 2160 Thr Cys Thr Ala Gly Cys Thr Cys Cys Ala Cys Thr Thr Thr Thr Ala 2165 2170 2175 Ala Cys Ala Gly Thr Ala Cys Thr Ala Gly Gly Gly Ala Gly Thr Thr 2180 2185 2190 Gly Cys Cys Thr Gly Gly Thr Thr Thr Cys Thr Thr Cys Thr Ala Cys 2195 2200 2205 Cys Ala Thr Thr Cys Thr Ala Ala Thr Gly Ala Thr Gly Gly Cys Thr 2210 2215 2220 Cys Thr Ala Ala Thr Thr Gly Thr Ala Cys Ala Gly Ala Gly Cys Cys 2225 2230 2235 2240 Thr Gly Thr Gly Thr Thr Gly Gly Thr Gly Thr Ala Thr Ala Gly Thr 2245 2250 2255 Ala Ala Cys Ala Thr Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala 2260 2265 2270 Ala Ala Thr Cys Thr Gly Gly Cys Ala Gly Thr Ala Thr Thr Gly Gly 2275 2280 2285 Cys Thr Ala Cys Gly Thr Cys Cys Cys Ala Thr Cys Thr Cys Ala Gly 2290 2295 2300 Thr Cys Thr Gly Gly Cys Cys Ala Ala Gly Thr Cys Ala Ala Gly Ala 2305 2310 2315 2320 Thr Thr Gly Cys Ala Cys Cys Cys Ala Cys Gly Gly Thr Thr Ala Cys 2325 2330 2335 Thr Gly Gly Gly Ala Ala Thr Ala Thr Thr Ala Gly Thr Ala Thr Thr 2340 2345 2350 Cys Cys Cys Ala Cys Cys Ala Ala Cys Thr Thr Thr Ala Gly Thr Ala 2355 2360 2365 Thr Gly Ala Gly Thr Ala Thr Thr Ala Gly Gly Ala Cys Ala Gly Ala 2370 2375 2380 Ala Thr Ala Thr Thr Thr Ala Cys Ala Gly Cys Thr Thr Thr Ala Cys 2385 2390 2395 2400 Ala Ala Cys Ala Cys Gly Cys Cys Thr Gly Thr Thr Ala Gly Thr Gly 2405 2410 2415 Thr Thr Gly Ala Thr Thr Gly Thr Gly Cys Cys Ala Cys Ala Thr Ala 2420 2425 2430 Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Thr Ala Ala Cys 2435 2440 2445 Thr Cys Thr Cys Gly Thr Thr Gly Thr Ala Ala Ala Cys Ala Ala Thr 2450 2455 2460 Thr Ala Cys Thr Cys Ala Cys Cys Cys Ala Gly Thr Ala Cys Ala Cys 2465 2470 2475 2480 Thr Gly Cys Ala Gly Cys Ala Thr Gly Thr Ala Ala Gly Ala Cys Cys 2485 2490 2495 Ala Thr Ala Gly Ala Gly Thr Cys Ala Gly Cys Ala Thr Thr Ala Cys 2500 2505 2510 Ala Ala Cys Thr Cys Ala Gly Cys Gly Cys Thr Ala Gly Gly Cys Thr 2515 2520 2525 Thr Gly Ala Gly Thr Cys Thr Gly Thr Thr Gly Ala Ala Gly Thr Thr 2530 2535 2540 Ala Ala Cys Thr Cys Thr Ala Thr Gly Cys Thr Thr Ala Cys Thr Ala 2545 2550 2555 2560 Thr Thr Thr Cys Thr Gly Ala Ala Gly Ala Gly Gly Cys Thr Cys Thr 2565 2570 2575 Ala Cys Ala Gly Thr Thr Ala Gly Cys Thr Ala Cys Cys Ala Thr Thr 2580 2585 2590 Ala Gly Thr Thr Cys Gly Thr Thr Thr Ala Ala Thr Gly Gly Thr Gly 2595 2600 2605 Ala Thr Gly Gly Ala Thr Ala Thr Ala Ala Thr Thr Thr Thr Ala Cys 2610 2615 2620 Thr Ala Ala Thr Gly Thr Gly Cys Thr Gly Gly Gly Thr Gly Thr Thr 2625 2630 2635 2640 Thr Cys Thr Gly Thr Gly Thr Ala Thr Gly Ala Thr Cys Cys Thr Gly 2645 2650 2655 Cys Ala Ala Gly Thr Gly Gly Cys Ala Gly Gly Gly Thr Gly Gly Thr 2660 2665 2670 Ala Cys Ala Ala Ala Ala Ala Ala Gly Gly Thr Cys Thr Thr Thr Thr 2675 2680 2685 Ala Thr Thr Gly Ala Ala Gly Ala Cys Cys Thr Gly Cys Thr Thr Thr 2690 2695 2700 Thr Thr Ala Ala Thr Ala Ala Ala Gly Thr Gly Gly Thr Thr Ala Cys 2705 2710 2715 2720 Thr Ala Ala Thr Gly Gly Cys Cys Thr Thr Gly Gly Thr Ala Cys Thr 2725 2730 2735 Gly Thr Thr Gly Ala Thr Gly Ala Ala Gly Ala Cys Thr Ala Thr Ala 2740 2745 2750 Ala Gly Cys Gly Cys Thr Gly Thr Thr Cys Thr Ala Ala Thr Gly Gly 2755 2760 2765 Thr Cys Gly Cys Thr Cys Thr Gly Thr Gly Gly Cys Ala Gly Ala Thr 2770 2775 2780 Cys Thr Ala Gly Thr Cys Thr Gly Thr Gly Cys Ala Cys Ala Gly Thr 2785 2790 2795 2800 Ala Thr Thr Ala Cys Thr Cys Thr Gly Gly Thr Gly Thr Cys Ala Thr 2805 2810 2815 Gly Gly Thr Ala Cys Thr Ala Cys Cys Thr Gly Gly Thr Gly Thr Thr 2820 2825 2830 Gly Thr Thr Gly Ala Cys Gly Cys Thr Gly Ala Gly Ala Ala Gly Cys 2835 2840 2845 Thr Thr Cys Ala Cys Ala Thr Gly Thr Ala Thr Ala Gly Thr Gly Cys 2850 2855 2860 Gly Thr Cys Thr Cys Thr Cys Ala Thr Cys Gly Gly Thr Gly Gly Thr 2865 2870 2875 2880 Ala Thr Gly Gly Thr Gly Cys Thr Ala Gly Gly Ala Gly Gly Thr Thr 2885 2890 2895 Thr Thr Ala Cys Thr Thr Cys Thr Gly Cys Ala Gly Cys Gly Gly Cys 2900 2905 2910 Ala Thr Thr Gly Cys Cys Thr Thr Thr Thr Ala Gly Cys Thr Ala Thr 2915 2920 2925 Gly Cys Thr Gly Thr Thr Cys Ala Ala Gly Cys Thr Ala Gly Ala Cys 2930 2935 2940 Thr Cys Ala Ala Thr Thr Ala Thr Cys Thr Thr Gly Cys Thr Cys Thr 2945 2950 2955 2960 Ala Cys Ala Gly Ala Cys Gly Gly Ala Thr Gly Thr Thr Cys Thr Ala 2965 2970 2975 Cys Ala Gly Cys Gly Gly Ala Ala Cys Cys Ala Gly Cys Ala Ala Thr 2980 2985 2990 Thr Gly Cys Thr Thr Gly Cys Thr Gly Ala Gly Thr Cys Thr Thr Thr 2995 3000 3005 Thr Ala Ala Cys Thr Cys Thr Gly Cys Thr Ala Thr Thr Gly Gly Thr 3010 3015 3020 Ala Ala Thr Ala Thr Ala Ala Cys Thr Thr Cys Ala Gly Cys Cys Thr 3025 3030 3035 3040 Thr Thr Gly Ala Gly Ala Gly Thr Gly Thr Thr Ala Ala Ala Gly Ala 3045 3050 3055 Gly Gly Cys Thr Ala Thr Thr Ala Gly Thr Cys Ala Ala Ala Cys Thr 3060 3065 3070 Thr Cys Cys Ala Ala Gly Gly Gly Thr Thr Thr Gly Ala Ala Cys Ala 3075 3080 3085 Cys Thr Gly Thr Gly Gly Cys Thr Cys Ala Thr Gly Cys Gly Cys Thr 3090 3095 3100 Thr Ala Cys Thr Ala Ala Gly Gly Thr Thr Cys Ala Ala Gly Ala Gly 3105 3110 3115 3120 Gly Thr Thr Gly Thr Thr Ala Ala Cys Thr Cys Gly Cys Ala Gly Gly 3125 3130 3135 Gly Thr Gly Cys Ala Gly Cys Thr Thr Thr Gly Ala Cys Thr Cys Ala 3140 3145 3150 Ala Cys Thr Thr Ala Cys Cys Gly Thr Ala Cys Ala Gly Cys Thr Gly 3155 3160 3165 Cys Ala Ala Cys Ala Cys Ala Ala Cys Thr Thr Cys Cys Ala Ala Gly 3170 3175 3180 Cys Cys Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Cys Thr Ala Thr 3185 3190 3195 3200 Thr Gly Ala Thr Gly Ala Cys Ala Thr Thr Thr Ala Cys Thr Cys Thr 3205 3210 3215 Cys Gly Ala Cys Thr Gly Gly Ala Cys Ala Thr Thr Cys Thr Thr Thr 3220 3225 3230 Cys Ala Gly Cys Cys Gly Ala Thr Gly Thr Thr Cys Ala Gly Gly Thr 3235 3240 3245 Thr Gly Ala Cys Cys Gly Thr Cys Thr Cys Ala Thr Cys Ala Cys Cys 3250 3255 3260 Gly Gly Cys Ala Gly Ala Thr Thr Ala Thr Cys Ala Gly Cys Ala Cys 3265 3270 3275 3280 Thr Thr Ala Ala Thr Gly Cys Thr Thr Thr Thr Gly Thr Thr Gly Cys 3285 3290 3295 Thr Cys Ala Ala Ala Cys Cys Cys Thr Cys Ala Cys Thr Ala Ala Gly 3300 3305 3310 Thr Ala Thr Ala Cys Thr Gly Ala Gly Gly Thr Thr Cys Ala Gly Gly 3315 3320 3325 Cys Thr Ala Gly Cys Ala Gly Gly Ala Ala Gly Thr Thr Ala Gly Cys 3330 3335 3340 Ala Cys Ala Gly Cys Ala Ala Ala Ala Gly Gly Thr Thr Ala Ala Thr 3345 3350 3355 3360 Gly Ala Gly Thr Gly Cys Gly Thr Thr Ala Ala Ala Thr Cys Gly Cys 3365 3370 3375 Ala Ala Thr Cys Thr Cys Ala Gly Cys Gly Thr Thr Ala Thr Gly Gly 3380 3385 3390 Thr Thr Thr Thr Thr Gly Thr Gly Gly Thr Gly Gly Thr Gly Ala Thr 3395 3400 3405 Gly Gly Cys Gly Ala Gly Cys Ala Cys Ala Thr Thr Thr Thr Cys Thr 3410 3415 3420 Cys Thr Cys Thr Gly Gly Thr Ala Cys Ala Gly Gly Cys Ala Gly Cys 3425 3430 3435 3440 Ala Cys Cys Thr Cys Ala Gly Gly Gly Cys Cys Thr Gly Cys Thr Gly 3445 3450 3455 Thr Thr Thr Thr Thr Ala Cys Ala Thr Ala Cys Ala Gly Thr Ala Cys 3460 3465 3470 Thr Thr Gly Thr Ala Cys Cys Gly Ala Gly Thr Gly Ala Thr Thr Thr 3475 3480 3485 Thr Gly Thr Ala Gly Ala Thr Gly Thr Thr Ala Thr Thr Gly Cys Cys 3490 3495 3500 Ala Thr Cys Gly Cys Thr Gly Gly Cys Thr Thr Ala Thr Gly Cys Gly 3505 3510 3515 3520 Thr Thr Ala Ala Cys Gly Ala Thr Gly Ala Ala Ala Thr Thr Gly Cys 3525 3530 3535 Cys Thr Thr Gly Ala Cys Thr Cys Thr Ala Cys Gly Thr Gly Ala Gly 3540 3545 3550 Cys Cys Thr Gly Gly Cys Thr Thr Ala Gly Thr Cys Thr Thr Gly Thr 3555 3560 3565 Thr Thr Ala Cys Gly Cys Ala Thr Gly Ala Ala Cys Thr Thr Cys Ala 3570 3575 3580 Ala Ala Ala Thr Cys Ala Thr Ala Cys Thr Gly Cys Gly Ala Thr Gly 3585 3590 3595 3600 Gly Ala Ala Thr Ala Thr Thr Thr Thr Gly Thr Thr Thr Cys Ala Thr 3605 3610 3615 Cys Gly Cys Gly Ala Cys Gly Thr Ala Thr Gly Thr Thr Thr Gly Ala 3620 3625 3630 Ala Cys Cys Thr Ala Gly Ala Ala Ala Ala Cys Cys Thr Ala Cys Cys 3635 3640 3645 Gly Thr Thr Ala Gly Thr Gly Ala Thr Thr Thr Thr Gly Thr Thr Cys 3650 3655 3660 Ala Ala Ala Thr Thr Gly Ala Gly Ala Gly Thr Thr Gly Thr Gly Thr 3665 3670 3675 3680 Gly Gly Thr Cys Ala Cys Cys Thr Ala Thr Gly Thr Cys Ala Ala Thr 3685 3690 3695 Thr Thr Gly Ala Cys Thr Ala Gly Ala Gly Ala Cys Cys Ala Ala Cys 3700 3705 3710 Thr Ala Cys Cys Ala Gly Ala Thr Gly Thr Ala Ala Thr Cys Cys Cys 3715 3720 3725 Ala Gly Ala Thr Thr Ala Cys Ala Thr Cys Gly Ala Thr Gly Thr Thr 3730 3735 3740 Ala Ala Cys Ala Ala Ala Ala Cys Ala Cys Thr Thr Gly Ala Thr Gly 3745 3750 3755 3760 Ala Gly Ala Thr Thr Thr Thr Ala Gly Cys Thr Thr Cys Thr Cys Thr 3765 3770 3775 Gly Cys Cys Cys Ala Ala Thr Ala Gly Ala Ala Cys Thr Gly Gly Thr 3780 3785 3790 Cys Cys Ala Ala Gly Thr Cys Thr Thr Cys Cys Thr Thr Thr Ala Gly 3795 3800 3805 Ala Thr Gly Thr Thr Thr Thr Thr Ala Ala Thr Gly Cys Cys Ala Cys 3810 3815 3820 Thr Thr Ala Thr Cys Thr Thr Ala Ala Thr Cys Thr Cys Ala Cys Thr 3825 3830 3835 3840 Gly Gly Thr Gly Ala Ala Ala Thr Thr Gly Cys Ala Gly Ala Thr Thr 3845 3850 3855 Thr Ala Gly Ala Gly Cys Ala Gly Cys Gly Thr Thr Cys Ala Gly Ala 3860 3865 3870 Gly Thr Cys Thr Cys Thr Cys Cys Gly Thr Ala Ala Thr Ala Cys Thr 3875 3880 3885 Ala Cys Ala Gly Ala Gly Gly Ala Gly Cys Thr Cys Cys Ala Ala Ala 3890 3895 3900 Gly Thr Cys Thr Thr Ala Thr Ala Thr Ala Thr Ala Ala Thr Ala Thr 3905 3910 3915 3920 Cys Ala Ala Cys Ala Ala Cys Ala Cys Ala Cys Thr Ala Gly Thr Thr 3925 3930 3935 Gly Ala Cys Cys Thr Thr Gly Ala Gly Thr Gly Gly Cys Thr Cys Ala 3940 3945 3950 Ala Cys Cys Gly Ala Gly Thr Thr Gly Ala Gly Ala Cys Ala Thr Ala 3955 3960 3965 Thr Ala Thr Cys Ala Ala Gly Thr Gly Gly Cys Cys Gly Thr Gly Gly 3970 3975 3980 Thr Gly Gly Gly Thr Thr Thr Gly Gly Thr Thr Gly Ala Thr Thr Ala 3985 3990 3995 4000 Thr Thr Thr Thr Cys Ala Thr Thr Gly Thr Thr Cys Thr Cys Ala Thr 4005 4010 4015 Cys Thr Thr Thr Gly Thr Thr Gly Thr Gly Thr Cys Ala Thr Thr Ala 4020 4025 4030 Cys Thr Ala Gly Thr Gly Thr Thr Cys Thr Gly Cys Thr Gly Cys Ala 4035 4040 4045 Thr Thr Thr Cys Cys Ala Cys Gly Gly Gly Thr Thr Gly Thr Thr Gly 4050 4055 4060 Thr Gly Gly Ala Thr Gly Cys Thr Gly Cys Gly Gly Cys Thr Gly Cys 4065 4070 4075 4080 Thr Gly Cys Thr Gly Thr Gly Cys Thr Thr Gly Thr Thr Thr Cys Thr 4085 4090 4095 Cys Ala Gly Gly Thr Thr Gly Thr Thr Gly Thr Ala Gly Gly Gly Gly 4100 4105 4110 Thr Cys Cys Thr Ala Gly Ala Cys Thr Thr Cys Ala Ala Cys Cys Thr 4115 4120 4125 Thr Ala Cys Gly Ala Ala Gly Thr Thr Thr Thr Thr Gly Ala Ala Ala 4130 4135 4140 Ala Gly Gly Thr Cys Cys Ala Cys Gly Thr Gly Cys Ala Gly Thr Gly 4145 4150 4155 4160 Ala Thr Gly Thr Thr Thr Cys Thr Thr Gly Gly Ala Cys Thr Thr Thr 4165 4170 4175 Thr Thr Cys Ala Ala Thr Ala Cys Ala Cys Gly Ala Thr Thr Gly Ala 4180 4185 4190 Cys Ala Cys Ala Gly Thr Thr Gly Thr Cys Ala Ala Ala Gly Ala Thr 4195 4200 4205 Gly Thr Cys Thr Cys Ala Ala Ala Gly Thr Cys Thr Gly Cys Thr Ala 4210 4215 4220 Ala Cys Thr Thr Gly Thr Cys Thr Thr Thr Gly Gly Ala Thr Gly Cys 4225 4230 4235 4240 Thr Gly Thr Cys Cys Ala Ala Gly Ala Gly Thr Thr Gly Gly Ala Gly 4245 4250 4255 Cys Thr Cys Ala Ala Thr Gly Thr Ala Gly Thr Thr Cys Cys Ala Ala 4260 4265 4270 Thr Thr Ala Gly Ala Cys Ala Ala Gly Cys Thr Thr Cys Ala Ala Ala 4275 4280 4285 Thr Gly Thr Gly Ala Cys Gly Gly Gly Thr Thr Thr Thr Cys Thr Thr 4290 4295 4300 Thr Thr Cys Ala Cys Cys Ala Gly Thr Gly Thr Thr Thr Thr Thr Ala 4305 4310 4315 4320 Thr Cys Thr Ala Cys Thr Thr Cys Thr Thr Thr Gly Cys Ala Cys Thr 4325 4330 4335 Gly Thr Thr Thr Ala Ala Ala Gly Cys Gly Thr Cys Thr Thr Cys Thr 4340 4345 4350 Thr Thr Gly Ala Gly Gly Cys Gly Cys Ala Ala Thr Thr Ala Thr Ala 4355 4360 4365 Thr Thr Ala Thr Gly Thr Thr Gly Gly Cys Ala Gly Cys Gly Cys Gly 4370 4375 4380 Thr Thr Thr Thr Gly Cys Thr Gly Thr Cys Ala Thr Thr Gly Thr Thr 4385 4390 4395 4400 Cys Thr Thr Thr Ala Thr Thr Gly Cys Cys Cys Ala Cys Thr Thr Thr 4405 4410 4415 Thr Ala Thr Ala Thr Thr Ala Thr Thr Gly Thr Gly Gly Thr Gly Cys 4420 4425 4430 Ala Thr Thr Thr Thr Thr Ala Gly Ala Thr Gly Cys Ala Ala Cys Thr 4435 4440 4445 Ala Thr Thr Ala Thr Thr Thr Gly Thr Thr Gly Cys Ala Cys Ala Cys 4450 4455 4460 Thr Thr Ala Thr Thr Gly Gly Cys Ala Gly Gly Cys Thr Thr Thr Gly 4465 4470 4475 4480 Thr Thr Thr Ala Gly Thr Cys Thr Gly Cys Thr Thr Thr Thr Ala Cys 4485 4490 4495 Thr Cys Cys Thr Gly Gly Cys Gly Cys Thr Ala Thr Ala Ala Ala Ala 4500 4505 4510 Ala Thr Gly Cys Gly Cys Thr Cys Thr Thr Thr Ala Thr Thr Ala Thr 4515 4520 4525 Thr Thr Thr Thr Ala Ala Thr Ala Cys Thr Ala Cys Gly Ala Cys Ala 4530 4535 4540 Cys Thr Thr Thr Cys Thr Thr Thr Cys Cys Thr Cys Ala Ala Thr Gly 4545 4550 4555 4560 Gly Thr Ala Ala Ala Gly Cys Ala Gly Cys Thr Thr Ala Thr Thr Ala 4565 4570 4575 Thr Gly Ala Cys Gly Gly Cys Ala Ala Ala Thr Cys Cys Ala Thr Thr 4580 4585 4590 Gly Thr Gly Ala Thr Thr Thr Thr Ala Gly Ala Ala Gly Gly Thr Gly 4595 4600 4605 Gly Thr Gly Ala Cys Cys Ala Thr Thr Ala Cys Ala Thr Cys Ala Cys 4610 4615 4620 Thr Thr Thr Thr Gly Gly Cys Ala Ala Cys Thr Cys Thr Thr Thr Thr 4625 4630 4635 4640 Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Thr Thr Ala Gly Thr Ala 4645 4650 4655 Gly Cys Ala Thr Cys Gly Ala Cys Thr Thr Gly Thr Ala Thr Cys Thr 4660 4665 4670 Ala Gly Cys Thr Ala Thr Ala Cys Gly Thr Gly Gly Gly Cys Gly Gly 4675 4680 4685 Cys Ala Ala Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Thr Ala Cys 4690 4695 4700 Ala Gly Cys Thr Gly Thr Thr Gly Cys Gly Ala Ala Cys Thr Gly Thr 4705 4710 4715 4720 Thr Gly Ala Gly Cys Thr Thr Cys Thr Thr Gly Ala Thr Gly Gly Cys 4725 4730 4735 Ala Ala Gly Ala Ala Gly Cys Thr Thr Thr Ala Thr Gly Thr Cys Thr 4740 4745 4750 Thr Thr Thr Cys Gly Cys Ala Ala Cys Ala Thr Cys Ala Ala Ala Thr 4755 4760 4765 Thr Gly Thr Thr Gly Gly Cys Ala Thr Thr Ala Cys Thr Ala Ala Thr 4770 4775 4780 Gly Cys Thr Gly Cys Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 4785 4790 4795 4800 Thr Thr Cys Ala Ala Cys Thr Ala Gly Ala Cys Gly Ala Gly Thr Ala 4805 4810 4815 Thr Gly Cys Thr Ala Cys Ala Ala Thr Thr Ala Gly Thr Gly Ala Ala 4820 4825 4830 Thr Gly Ala Thr Ala Ala Thr Gly Gly Thr Cys Thr Ala Gly Thr Ala 4835 4840 4845 Gly Thr Thr Ala Ala Thr Gly Thr Thr Ala Thr Ala Cys Thr Thr Thr 4850 4855 4860 Gly Gly Cys Thr Thr Thr Thr Cys Gly Thr Ala Cys Thr Cys Thr Thr 4865 4870 4875 4880 Thr Thr Thr Cys Cys Thr Gly Cys Thr Thr Ala Thr Thr Ala Thr Ala 4885 4890 4895 Ala Gly Cys Ala Thr Thr Ala Cys Thr Thr Thr Cys Gly Thr Cys Cys 4900 4905 4910 Ala Ala Thr Thr Gly Gly Thr Thr Ala Ala Thr Cys Thr Gly Thr Gly 4915 4920 4925 Cys Thr Thr Cys Ala Cys Thr Thr Gly Thr Cys Ala Cys Cys Gly Gly 4930 4935 4940 Thr Thr Gly Thr Gly Thr Ala Ala Thr Ala Gly Cys Gly Cys Ala Gly 4945 4950 4955 4960 Thr Thr Thr Ala Cys Ala Cys Ala Cys Cys Thr Ala Thr Ala Gly Gly 4965 4970 4975 Gly Cys Gly Thr Thr Thr Gly Thr Ala Thr Ala Gly Ala Gly Thr Thr 4980 4985 4990 Thr Ala Thr Ala Ala Gly Thr Cys Thr Thr Ala Cys Ala Thr Gly Cys 4995 5000 5005 Ala Ala Ala Thr Ala Gly Ala Cys Cys Cys Cys Cys Thr Cys Cys Cys 5010 5015 5020 Thr Ala Gly Thr Ala Cys Thr Gly Thr Thr Ala Thr Thr Gly Ala Cys 5025 5030 5035 5040 Gly Thr Ala Thr Ala Ala Ala Cys Gly Ala Ala Ala Thr Ala Thr Gly 5045 5050 5055 Thr Cys Thr Ala Ala Cys Gly Gly Thr Thr Cys Thr Ala Thr Thr Cys 5060 5065 5070 Cys Cys Gly Thr Thr Gly Ala Thr Gly Ala Gly Gly Thr Gly Ala Thr 5075 5080 5085 Thr Cys Ala Ala Cys Ala Cys Cys Thr Thr Ala Gly Ala Ala Ala Cys 5090 5095 5100 Thr Gly Gly Ala Ala Thr Thr Thr Cys Ala Cys Ala Thr Gly Gly Ala 5105 5110 5115 5120 Ala Thr Ala Thr Cys Ala Thr Ala Cys Thr Gly Ala Cys Gly Ala Thr 5125 5130 5135 Ala Cys Thr Ala Cys Thr Thr Gly Thr Ala Gly Thr Gly Cys Thr Thr 5140 5145 5150 Cys Ala Gly Thr Ala Thr Gly Gly Cys Cys Ala Thr Thr Ala Cys Ala 5155 5160 5165 Ala Gly Thr Ala Cys Thr Cys Thr Gly Cys Gly Thr Thr Cys Thr Thr 5170 5175 5180 Gly Thr Ala Thr Gly Gly Thr Gly Thr Cys Ala Ala Gly Ala Thr Gly 5185 5190 5195 5200 Gly Cys Thr Ala Thr Thr Cys Thr Ala Thr Gly Gly Ala Thr Ala Cys 5205 5210 5215 Thr Thr Thr Gly Gly Cys Cys Thr Cys Thr Thr Gly Thr Gly Thr Thr 5220 5225 5230 Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Cys Thr Thr Thr Thr Thr 5235 5240 5245 Gly Ala Thr Gly Cys Ala Thr Gly Gly Gly Cys Thr Ala Gly Cys Thr 5250 5255 5260 Thr Thr Cys Ala Gly Gly Thr Cys Ala Ala Thr Thr Gly Gly Gly Thr 5265 5270 5275 5280 Cys Thr Thr Thr Thr Thr Thr Gly Cys Thr Thr Thr Cys Ala Gly Cys 5285 5290 5295 Ala Thr Cys Cys Thr Thr Ala Thr Gly Gly Cys Thr Thr Gly Cys Ala 5300 5305 5310 Thr Cys Ala Cys Thr Cys Thr Thr Ala Thr Gly Cys Thr Gly Thr Gly 5315 5320 5325 Gly Ala Thr Ala Ala Thr Gly Thr Ala Cys Thr Thr Thr Gly Thr Cys 5330 5335 5340 Ala Ala Thr Ala Gly Cys Ala Thr Thr Cys Gly Gly Thr Thr Gly Thr 5345 5350 5355 5360 Gly Gly Cys Gly Cys Ala Gly Gly Ala Cys Ala Cys Ala Thr Thr Cys 5365 5370 5375 Thr Thr Gly Gly Thr Gly Gly Thr Cys Thr Thr Thr Cys Ala Ala Thr 5380 5385 5390 Cys Cys Thr Gly Ala Ala Ala Cys Ala Gly Ala Cys Gly Cys Gly Cys 5395 5400 5405 Thr Thr Cys Thr Cys Ala Cys Thr Ala Cys Thr Thr Cys Thr Gly Thr 5410 5415 5420 Gly Ala Thr Gly Gly Gly Cys Cys Gly Ala Cys Ala Gly Gly Thr Cys 5425 5430 5435 5440 Thr Gly Cys Ala Thr Thr Cys Cys Ala Gly Thr Gly Cys Thr Thr Gly 5445 5450 5455 Gly Ala Gly Cys Ala Cys Cys Ala Ala Cys Thr Gly Gly Thr Gly Thr 5460 5465 5470 Ala Ala Cys Gly Cys Thr Ala Ala Cys Ala Cys Thr Cys Cys Thr Thr 5475 5480 5485 Ala Gly Thr Gly Gly Thr Ala Cys Ala Thr Thr Gly Cys Thr Thr Gly 5490 5495 5500 Thr Ala Gly Ala Gly Gly Gly Cys Thr Ala Thr Ala Ala Gly Gly Thr 5505 5510 5515 5520 Thr Gly Cys Thr Ala Cys Thr Gly Gly Cys Gly Thr Ala Cys Ala Gly 5525 5530 5535 Gly Thr Ala Ala Gly Thr Cys Ala Ala Thr Thr Ala Cys Cys Thr Ala 5540 5545 5550 Ala Thr Thr Thr Cys Gly Thr Cys Ala Cys Ala Gly Thr Cys Gly Cys 5555 5560 5565 Cys Ala Ala Gly Gly Cys Cys Ala Cys Thr Ala Cys Ala Ala Cys Ala 5570 5575 5580 Ala Thr Thr Gly Thr Cys Thr Ala Cys Gly Gly Ala Cys Gly Thr Gly 5585 5590 5595 5600 Thr Thr Gly Gly Thr Cys Gly Thr Thr Cys Ala Gly Thr Cys Ala Ala 5605 5610 5615 Thr Gly Cys Thr Thr Cys Ala Thr Cys Thr Gly Gly Cys Ala Cys Thr 5620 5625 5630 Gly Gly Thr Thr Gly Gly Gly Cys Thr Thr Thr Cys Thr Ala Thr Gly 5635 5640 5645 Thr Cys Cys Gly Gly Thr Cys Cys Ala Ala Ala Cys Ala Cys Gly Gly 5650 5655 5660 Cys Gly Ala Cys Thr Ala Cys Thr Cys Ala Gly Cys Thr Gly Thr Gly 5665 5670 5675 5680 Ala Gly Thr Ala Ala Thr Cys Cys Gly Ala Gly Thr Thr Cys Gly Gly 5685 5690 5695 Thr Thr Cys Thr Cys Ala Cys Ala Gly Ala Thr Ala Gly Thr Gly Ala 5700 5705 5710 Gly Ala Ala Ala Gly Thr Gly Cys Thr Thr Cys Ala Thr Thr Thr Ala 5715 5720 5725 Gly Thr Cys Thr Ala Ala Ala Cys Ala Gly Ala Ala Ala Cys Thr Thr 5730 5735 5740 Thr Ala Thr Gly Gly Cys Thr Thr Cys Thr Gly Thr Cys Ala Gly Thr 5745 5750 5755 5760 Thr Thr Thr Cys Ala Gly Gly Ala Thr Cys Gly Thr Gly Gly Cys Cys 5765 5770 5775 Gly Cys Ala Ala Ala Cys Gly Gly Gly Thr Gly Cys Cys Ala Thr Thr 5780 5785 5790 Ala Thr Cys Cys Cys Thr Cys Thr Ala Thr Gly Cys Cys Cys Cys Thr 5795 5800 5805 Cys Thr Thr Ala Gly Gly Gly Thr Thr Ala Cys Thr Ala Ala Thr Gly 5810 5815 5820 Ala Cys Ala Ala Ala Cys Cys Cys Cys Thr Thr Thr Cys Thr Ala Ala 5825 5830 5835 5840 Gly Gly Thr Ala Cys Thr Thr Gly Cys Ala Ala Ala Thr Ala Ala Thr 5845 5850 5855 Gly Cys Thr Gly Thr Ala Cys Cys Cys Ala Cys Thr Ala Ala Thr Ala 5860 5865 5870 Ala Ala Gly Gly Ala Ala Ala Thr Ala Ala Gly Gly Ala Cys Cys Ala 5875 5880 5885 Gly Cys Ala Ala Ala Thr Thr Gly Gly Ala Thr Ala Cys Thr Gly Gly 5890 5895 5900 Ala Ala Thr Gly Ala Gly Cys Ala Ala Ala Thr Thr Cys Gly Cys Thr 5905 5910 5915 5920 Gly Gly Cys Gly Cys Ala Thr Gly Cys Gly Cys Cys Gly Thr Gly Gly 5925 5930 5935 Thr Gly Ala Gly Cys Gly Ala Ala Thr Thr Gly Ala Ala Cys Ala Ala 5940 5945 5950 Cys Cys Thr Thr Cys Cys Ala Ala Thr Thr Gly Gly Cys Ala Thr Thr 5955 5960 5965 Thr Cys Thr Ala Cys Thr Ala Cys Cys Thr Cys Gly Gly Ala Ala Cys 5970 5975 5980 Ala Gly Gly Ala Cys Cys Thr Cys Ala Cys Gly Cys Cys Gly Ala Cys 5985 5990 5995 6000 Cys Thr Cys Cys Gly Cys Thr Ala Thr Ala Gly Gly Ala Cys Thr Cys 6005 6010 6015 Gly Thr Ala Cys Thr Gly Ala Gly Gly Gly Thr Gly Thr Thr Thr Thr 6020 6025 6030 Cys Thr Gly Gly Gly Thr Thr Gly Cys Thr Ala Ala Ala Gly Ala Ala 6035 6040 6045 Gly Gly Cys Gly Cys Ala Ala Ala Gly Ala Cys Thr Gly Ala Ala Cys 6050 6055 6060 Cys Cys Ala Cys Thr Ala Ala Cys Cys Thr Gly Gly Gly Thr Gly Thr 6065 6070 6075 6080 Cys Ala Gly Ala Ala Ala Gly Gly Cys Gly Thr Cys Thr Gly Ala Ala 6085 6090 6095 Ala Ala Gly Cys Cys Ala Ala Thr Thr Ala Thr Thr Cys Cys Ala Ala 6100 6105 6110 Ala Thr Thr Thr Cys Thr Cys Thr Cys Ala Ala Cys Ala Gly Cys Thr 6115 6120 6125 Thr Cys Cys Cys Ala Gly Cys Gly Thr Ala Gly Thr Thr Gly Ala Gly 6130 6135 6140 Ala Thr Thr Gly Thr Thr Gly Ala Ala Cys Cys Thr Ala Ala Cys Ala 6145 6150 6155 6160 Cys Ala Cys Cys Thr Cys Cys Thr Ala Cys Thr Thr Cys Ala Cys Gly 6165 6170 6175 Thr Gly Cys Ala Ala Ala Thr Thr Cys Ala Cys Gly Thr Ala Gly Cys 6180 6185 6190 Ala Gly Gly Ala Gly Thr Cys Gly Thr Gly Gly Thr Ala Ala Thr Gly 6195 6200 6205 Gly Cys Ala Ala Cys Ala Ala Cys Ala Gly Gly Thr Cys Cys Ala Gly 6210 6215 6220 Ala Thr Cys Thr Cys Cys Ala Ala Gly Thr Ala Ala Cys Ala Ala Cys 6225 6230 6235 6240 Ala Gly Ala Gly Gly Cys Ala Ala Thr Ala Ala Cys Cys Ala Gly Thr 6245 6250 6255 Cys Cys Cys Gly Cys Gly Gly Thr Ala Ala Thr Thr Cys Ala Cys Ala 6260 6265 6270 Gly Ala Ala Thr Cys Gly Thr Gly Gly Ala Ala Ala Thr Ala Ala Cys 6275 6280 6285 Cys Ala Gly Gly Gly Thr Cys Gly Thr Gly Gly Ala Gly Cys Thr Thr 6290 6295 6300 Cys Thr Cys Ala Gly Ala Ala Cys Ala Gly Ala Gly Gly Ala Gly Gly 6305 6310 6315 6320 Cys Ala Ala Thr Ala Ala Thr Ala Ala Thr Ala Ala Cys Ala Ala Thr 6325 6330 6335 Ala Ala Cys Ala Ala Gly Thr Cys Thr Cys Gly Thr Ala Ala Cys Cys 6340 6345 6350 Ala Gly Thr Cys Cys Ala Ala Gly Ala Ala Cys Ala Gly Ala Ala Ala 6355 6360 6365 Cys Cys Ala Gly Thr Cys Ala Ala Ala Thr Gly Ala Cys Cys Gly Thr 6370 6375 6380 Gly Gly Thr Gly Gly Thr Gly Thr Ala Ala Cys Ala Thr Cys Ala Cys 6385 6390 6395 6400 Gly Cys Gly Ala Thr Gly Ala Thr Cys Thr Gly Gly Thr Gly Gly Cys 6405 6410 6415 Thr Gly Cys Thr Gly Thr Cys Ala Ala Gly Gly Ala Thr Gly Cys Cys 6420 6425 6430 Cys Thr Thr Ala Ala Ala Thr Cys Thr Thr Thr Gly Gly Gly Thr Ala 6435 6440 6445 Thr Thr Gly Gly Cys Gly Ala Ala Ala Ala Cys Cys Cys Thr Gly Ala 6450 6455 6460 Cys Ala Ala Gly Cys Thr Thr Ala Ala Gly Cys Ala Ala Cys Ala Gly 6465 6470 6475 6480 Cys Ala Gly Ala Ala Gly Cys Cys Cys Ala Ala Ala Cys Ala Gly Gly 6485 6490 6495 Ala Ala Ala Gly Gly Thr Cys Thr Gly Ala Cys Ala Gly Cys Ala Gly 6500 6505 6510 Cys Gly Gly Cys Ala Ala Ala Ala Ala Thr Ala Cys Ala Cys Cys Thr 6515 6520 6525 Ala Ala Gly Ala Ala Gly Ala Ala Cys Ala Ala Ala Thr Cys Cys Ala 6530 6535 6540 Gly Ala Gly Cys Cys Ala Cys Thr Thr Cys Gly Ala Ala Ala Gly Ala 6545 6550 6555 6560 Ala Cys Gly Thr Gly Ala Cys Cys Thr Cys Ala Ala Ala Gly Ala Cys 6565 6570 6575 Ala Thr Cys Cys Cys Ala Gly Ala Gly Thr Gly Gly Ala Gly Gly Ala 6580 6585 6590 Gly Ala Ala Thr Thr Cys Cys Cys Ala Ala Gly Gly Gly Cys Gly Ala 6595 6600 6605 Ala Ala Ala Thr Ala Gly Cys Gly Thr Ala Gly Cys Ala Gly Cys Thr 6610 6615 6620 Thr Gly Cys Thr Thr Cys Gly Gly Ala Cys Cys Cys Ala Gly Gly Gly 6625 6630 6635 6640 Gly Ala Gly Gly Cys Thr Thr Cys Ala Ala Ala Ala Ala Thr Thr Thr 6645 6650 6655 Thr Gly Gly Ala Gly Ala Thr Gly Cys Gly Gly Ala Ala Thr Thr Thr 6660 6665 6670 Gly Thr Cys Gly Ala Ala Ala Ala Ala Gly Gly Thr Gly Thr Thr Gly 6675 6680 6685 Ala Thr Gly Cys Cys Thr Cys Ala Gly Gly Cys Thr Ala Thr Gly Cys 6690 6695 6700 Thr Cys Ala Gly Ala Thr Cys Gly Cys Cys Ala Gly Thr Thr Thr Ala 6705 6710 6715 6720 Gly Cys Ala Cys Cys Ala Ala Ala Thr Gly Thr Thr Gly Cys Ala Gly 6725 6730 6735 Cys Ala Thr Thr Gly Cys Thr Cys Thr Thr Thr Gly Gly Thr Gly Gly 6740 6745 6750 Thr Ala Ala Thr Gly Thr Gly Gly Cys Thr Gly Thr Thr Cys Gly Thr 6755 6760 6765 Gly Ala Gly Cys Thr Ala Gly Cys Gly Gly Ala Cys Thr Cys Thr Thr 6770 6775 6780 Ala Cys Gly Ala Gly Ala Thr Thr Ala Cys Ala Thr Ala Thr Ala Ala 6785 6790 6795 6800 Thr Thr Ala Thr Ala Ala Ala Ala Thr Gly Ala Cys Thr Gly Thr Gly 6805 6810 6815 Cys Cys Ala Ala Ala Gly Thr Cys Thr Gly Ala Thr Cys Cys Ala Ala 6820 6825 6830 Ala Thr Gly Thr Ala Gly Ala Gly Cys Thr Thr Cys Thr Thr Gly Thr 6835 6840 6845 Thr Thr Cys Ala Cys Ala Gly Gly Thr Gly Gly Ala Thr Gly Cys Ala 6850 6855 6860 Thr Thr Thr Ala Ala Ala Ala Cys Thr Gly Gly Gly Ala Ala Thr Gly 6865 6870 6875 6880 Cys Ala Ala Ala Ala Cys Cys Cys Cys Ala Gly Ala Gly Ala Ala Ala 6885 6890 6895 Gly Ala Ala Gly Gly Ala Ala Ala Ala Gly Ala Ala Gly Ala Ala Cys 6900 6905 6910 Ala Ala Gly Cys Gly Thr Gly Ala Ala Ala Cys Cys Ala Cys Gly Cys 6915 6920 6925 Ala Gly Cys Ala Gly Cys Thr Gly Ala Ala Thr Gly Ala Ala Gly Ala 6930 6935 6940 Gly Gly Cys Cys Ala Thr Cys Thr Ala Cys Gly Ala Thr Gly Ala Thr 6945 6950 6955 6960 Gly Thr Gly Gly Gly Thr Gly Thr Gly Cys Cys Ala Thr Cys Thr Gly 6965 6970 6975 Ala Thr Gly Thr Gly Ala Cys Thr Cys Ala Thr Gly Cys Cys Ala Ala 6980 6985 6990 Thr Thr Thr Gly Gly Ala Ala Thr Gly Gly Gly Ala Cys Ala Cys Ala 6995 7000 7005 Gly Cys Thr Gly Thr Thr Gly Ala Thr Gly Gly Thr Gly Gly Thr Gly 7010 7015 7020 Ala Cys Ala Cys Gly Gly Cys Cys Gly Thr Thr Gly Ala Ala Ala Thr 7025 7030 7035 7040 Thr Ala Thr Cys Ala Ala Cys Gly Ala Gly Ala Thr Cys Thr Thr Cys 7045 7050 7055 Gly Ala Cys Ala Cys Ala Gly Gly Ala Ala Ala Thr Thr Ala Ala Ala 7060 7065 7070 Cys Ala Ala Thr Gly Thr Thr Thr Gly Ala Cys Thr Gly Gly Cys Thr 7075 7080 7085 Thr Ala Thr Cys Cys Thr Gly Gly Cys Thr Ala Thr Gly Thr Cys Cys 7090 7095 7100 Cys Ala Gly Gly Gly Thr Ala Gly Thr Gly Cys Cys Ala Thr Thr Ala 7105 7110 7115 7120 Cys Ala Cys Thr Gly Thr Thr Ala Thr Thr Ala Cys Thr Gly Ala Gly 7125 7130 7135 Thr Gly Thr Thr Thr Thr Thr Cys Thr Ala Gly Cys Gly Ala Cys Thr 7140 7145 7150 Thr Gly Gly Cys Thr Gly Cys Thr Gly Gly Gly Cys Thr Ala Thr Gly 7155 7160 7165 Gly Cys Thr Thr Thr Gly Cys Cys Cys Thr Cys Thr Ala Ala Cys Thr 7170 7175 7180 Ala Gly Cys Gly Gly Thr Cys Thr Thr Gly Gly Thr Cys Thr Thr Gly 7185 7190 7195 7200 Cys Ala Cys Ala Cys Ala Ala Cys Gly Gly Thr Ala Ala Gly Cys Cys 7205 7210 7215 Ala Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Cys Ala Gly Thr Gly 7220 7225 7230 Cys Ala Ala Gly Ala Ala Gly Gly Ala Thr Ala Thr Thr Ala Cys Cys 7235 7240 7245 Ala Thr Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Thr Gly Ala Gly 7250 7255 7260 Gly Gly Gly Ala Ala Cys Gly Cys Ala Gly Thr Ala Cys Cys Thr Thr 7265 7270 7275 7280 Thr Thr Cys Ala Thr Cys Thr Ala Ala Ala Cys Cys Thr Thr Thr Gly 7285 7290 7295 Cys Ala Cys Gly Ala Gly Thr Ala Ala Thr Cys Ala Ala Ala Gly Ala 7300 7305 7310 Thr Cys Cys Gly Cys Thr Thr Gly Ala Cys Gly Ala Gly Cys Cys Thr 7315 7320 7325 Ala Thr Ala Thr Gly Gly Ala Ala Gly Ala Gly Cys Gly Thr Gly Cys 7330 7335 7340 Cys Ala Gly Gly Thr Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 7345 7350 7355 7360 Gly Gly Ala Cys Thr Gly Thr Thr Ala Gly Thr Ala Ala Cys Thr Gly 7365 7370 7375 Ala Ala Gly Ala Cys Cys Thr Gly Ala Cys Gly Gly Thr Gly Thr Thr 7380 7385 7390 Gly Ala Thr Ala Thr Gly Gly Ala Thr Ala Cys Ala Cys 7395 7400 7405 <210> 34 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 spike protein <400> 34 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Thr 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Gln Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 35 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 ORF3 <400> 35 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Tyr Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 36 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 envelope protein <400> 36 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Ser Ser Thr Val Ile Asp Val 65 70 75 <210> 37 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 membrane protein <400> 37 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 38 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 nucleocapsid protein <400> 38 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 39 <211> 7405 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P20 complete cds <400> 39 Ala Thr Gly Ala Ala Gly Thr Cys Thr Thr Thr Ala Ala Cys Cys Thr 1 5 10 15 Ala Cys Thr Thr Cys Thr Gly Gly Thr Thr Gly Thr Thr Cys Thr Thr 20 25 30 Ala Cys Cys Ala Gly Thr Ala Cys Thr Thr Thr Cys Ala Ala Cys Ala 35 40 45 Cys Thr Thr Ala Gly Cys Cys Thr Ala Cys Cys Ala Cys Ala Ala Gly 50 55 60 Ala Thr Gly Thr Cys Ala Cys Cys Ala Gly Gly Thr Gly Cys Thr Cys 65 70 75 80 Ala Gly Cys Thr Ala Ala Cys Ala Cys Thr Ala Ala Thr Thr Thr Thr 85 90 95 Ala Gly Gly Cys Gly Gly Thr Thr Cys Thr Thr Thr Thr Cys Ala Ala 100 105 110 Ala Ala Thr Thr Thr Ala Ala Thr Gly Thr Thr Cys Ala Gly Gly Cys 115 120 125 Gly Cys Cys Thr Gly Cys Ala Gly Thr Thr Gly Thr Thr Gly Thr Ala 130 135 140 Cys Thr Gly Gly Gly Cys Gly Gly Thr Thr Ala Thr Cys Thr Ala Cys 145 150 155 160 Cys Thr Ala Thr Thr Gly Gly Thr Gly Ala Ala Ala Ala Cys Cys Ala 165 170 175 Gly Gly Gly Thr Gly Thr Cys Ala Ala Thr Thr Cys Ala Ala Cys Thr 180 185 190 Thr Gly Gly Thr Ala Cys Thr Gly Thr Gly Cys Thr Gly Gly Cys Cys 195 200 205 Ala Ala Cys Ala Thr Cys Cys Ala Ala Cys Thr Gly Cys Thr Ala Gly 210 215 220 Thr Gly Gly Cys Gly Thr Thr Cys Ala Thr Gly Gly Thr Ala Thr Cys 225 230 235 240 Thr Thr Thr Gly Thr Thr Ala Gly Cys Cys Ala Thr Ala Thr Thr Ala 245 250 255 Gly Ala Gly Gly Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Thr Thr 260 265 270 Thr Gly Ala Gly Ala Thr Thr Gly Gly Cys Ala Thr Thr Thr Cys Gly 275 280 285 Cys Ala Ala Gly Ala Gly Cys Cys Thr Thr Thr Thr Gly Ala Cys Cys 290 295 300 Cys Thr Ala Gly Thr Gly Gly Thr Thr Ala Cys Cys Ala Gly Cys Thr 305 310 315 320 Thr Thr Ala Thr Thr Thr Ala Cys Ala Thr Ala Ala Gly Gly Cys Thr 325 330 335 Ala Cys Thr Ala Ala Cys Gly Gly Thr Ala Ala Cys Ala Cys Thr Ala 340 345 350 Ala Thr Gly Cys Thr Ala Cys Thr Gly Cys Gly Cys Gly Ala Cys Thr 355 360 365 Gly Cys Gly Cys Ala Thr Thr Thr Gly Cys Cys Ala Gly Thr Thr Thr 370 375 380 Cys Cys Thr Ala Gly Cys Ala Thr Thr Ala Ala Ala Ala Cys Ala Thr 385 390 395 400 Thr Gly Gly Gly Cys Cys Cys Cys Ala Cys Thr Gly Cys Thr Ala Ala 405 410 415 Thr Ala Ala Thr Gly Ala Thr Gly Thr Thr Ala Cys Ala Ala Cys Ala 420 425 430 Gly Gly Thr Cys Gly Thr Ala Ala Thr Thr Gly Cys Cys Thr Ala Thr 435 440 445 Thr Thr Ala Ala Cys Ala Ala Ala Gly Cys Cys Ala Thr Cys Cys Cys 450 455 460 Ala Gly Cys Thr Cys Ala Thr Ala Thr Gly Ala Gly Thr Gly Ala Ala 465 470 475 480 Cys Ala Thr Ala Gly Thr Gly Thr Thr Gly Thr Cys Gly Gly Cys Ala 485 490 495 Thr Ala Ala Cys Ala Thr Gly Gly Gly Ala Thr Ala Ala Thr Gly Ala 500 505 510 Thr Cys Gly Thr Gly Thr Cys Ala Cys Thr Gly Thr Cys Thr Thr Thr 515 520 525 Thr Cys Thr Gly Ala Cys Ala Ala Gly Ala Thr Cys Thr Ala Thr Thr 530 535 540 Ala Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Ala Ala Ala Ala Ala 545 550 555 560 Thr Gly Ala Thr Thr Gly Gly Thr Cys Cys Cys Gly Thr Gly Thr Thr 565 570 575 Gly Cys Gly Ala Cys Ala Ala Ala Gly Thr Gly Thr Thr Ala Cys Ala 580 585 590 Ala Cys Ala Gly Thr Gly Gly Ala Gly Gly Thr Thr Gly Thr Gly Cys 595 600 605 Thr Ala Thr Gly Cys Ala Ala Thr Ala Thr Gly Thr Thr Thr Ala Cys 610 615 620 Gly Ala Ala Cys Cys Cys Ala Cys Cys Thr Ala Thr Thr Ala Cys Ala 625 630 635 640 Thr Gly Cys Thr Thr Ala Ala Thr Gly Thr Thr Ala Cys Thr Ala Gly 645 650 655 Thr Gly Cys Thr Gly Gly Thr Gly Ala Gly Gly Ala Thr Gly Gly Thr 660 665 670 Ala Thr Thr Thr Cys Thr Thr Ala Thr Cys Ala Ala Cys Cys Cys Thr 675 680 685 Gly Thr Ala Cys Ala Gly Cys Thr Ala Ala Thr Thr Gly Cys Ala Thr 690 695 700 Thr Gly Gly Thr Thr Ala Thr Gly Cys Thr Gly Cys Cys Ala Ala Thr 705 710 715 720 Gly Thr Ala Thr Thr Thr Gly Cys Thr Ala Cys Thr Gly Ala Gly Cys 725 730 735 Cys Cys Ala Ala Thr Gly Gly Cys Cys Ala Cys Ala Thr Ala Cys Cys 740 745 750 Ala Gly Ala Ala Gly Gly Thr Thr Thr Thr Ala Gly Thr Thr Thr Thr 755 760 765 Ala Ala Thr Ala Ala Thr Thr Gly Gly Thr Thr Thr Cys Thr Thr Thr 770 775 780 Thr Gly Thr Cys Cys Ala Ala Thr Gly Ala Thr Thr Cys Cys Ala Cys 785 790 795 800 Thr Thr Thr Gly Gly Thr Gly Cys Ala Thr Gly Gly Thr Ala Cys Gly 805 810 815 Gly Thr Gly Gly Thr Thr Thr Cys Cys Ala Ala Cys Cys Ala Ala Cys 820 825 830 Cys Ala Thr Thr Gly Thr Thr Gly Gly Thr Cys Ala Ala Thr Thr Gly 835 840 845 Thr Cys Thr Thr Thr Thr Gly Gly Cys Cys Ala Thr Thr Cys Cys Thr 850 855 860 Ala Ala Gly Ala Thr Thr Thr Ala Thr Gly Gly Ala Cys Thr Ala Gly 865 870 875 880 Gly Cys Cys Ala Ala Thr Thr Thr Thr Thr Cys Thr Cys Cys Thr Thr 885 890 895 Thr Ala Ala Thr Cys Ala Ala Ala Cys Gly Ala Thr Cys Gly Ala Thr 900 905 910 Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Ala Gly 915 920 925 Cys Thr Gly Cys Thr Gly Thr Gly Cys Ala Gly Cys Gly Thr Gly Cys 930 935 940 Ala Cys Cys Ala Gly Ala Gly Gly Cys Thr Cys Thr Gly Ala Gly Gly 945 950 955 960 Thr Thr Thr Ala Ala Thr Ala Thr Thr Ala Ala Thr Gly Ala Cys Ala 965 970 975 Cys Cys Thr Cys Thr Gly Thr Cys Ala Thr Thr Cys Thr Thr Gly Cys 980 985 990 Thr Gly Ala Ala Gly Gly Cys Thr Cys Ala Ala Thr Thr Gly Thr Ala 995 1000 1005 Cys Thr Thr Cys Ala Thr Ala Cys Thr Gly Cys Thr Thr Thr Ala Gly 1010 1015 1020 Gly Ala Ala Cys Ala Ala Ala Thr Thr Thr Thr Thr Cys Thr Thr Thr 1025 1030 1035 1040 Thr Gly Thr Thr Thr Gly Cys Ala Gly Thr Ala Ala Thr Thr Cys Cys 1045 1050 1055 Thr Cys Ala Ala Ala Thr Cys Cys Thr Cys Ala Cys Thr Thr Ala Gly 1060 1065 1070 Cys Cys Ala Cys Cys Thr Thr Cys Gly Cys Cys Ala Thr Ala Cys Cys 1075 1080 1085 Thr Thr Thr Gly Gly Gly Thr Gly Cys Thr Ala Cys Cys Cys Ala Ala 1090 1095 1100 Gly Thr Ala Cys Cys Cys Thr Ala Thr Thr Ala Thr Thr Gly Thr Thr 1105 1110 1115 1120 Thr Thr Thr Thr Thr Ala Ala Ala Gly Thr Gly Gly Ala Thr Ala Cys 1125 1130 1135 Thr Thr Ala Cys Ala Ala Cys Thr Cys Cys Ala Cys Thr Gly Thr Thr 1140 1145 1150 Thr Ala Thr Ala Ala Ala Thr Thr Cys Thr Thr Gly Gly Cys Thr Gly 1155 1160 1165 Thr Thr Thr Thr Ala Cys Cys Thr Cys Cys Ala Ala Cys Cys Gly Thr 1170 1175 1180 Cys Ala Gly Gly Gly Ala Ala Ala Thr Thr Gly Thr Cys Ala Thr Cys 1185 1190 1195 1200 Ala Cys Cys Ala Ala Gly Thr Ala Thr Gly Gly Thr Gly Ala Thr Gly 1205 1210 1215 Thr Thr Thr Ala Thr Gly Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1220 1225 1230 Thr Gly Gly Ala Thr Ala Cys Thr Thr Gly Cys Ala Thr Cys Thr Cys 1235 1240 1245 Gly Gly Thr Thr Thr Gly Thr Thr Gly Gly Ala Thr Gly Cys Thr Gly 1250 1255 1260 Thr Cys Ala Cys Ala Ala Thr Thr Ala Ala Thr Thr Thr Cys Ala Cys 1265 1270 1275 1280 Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Ala Cys Thr Gly Ala Cys 1285 1290 1295 Gly Ala Thr Gly Ala Thr Gly Thr Thr Thr Cys Thr Gly Gly Thr Thr 1300 1305 1310 Thr Thr Thr Gly Gly Ala Cys Cys Ala Thr Ala Gly Cys Ala Thr Cys 1315 1320 1325 Gly Ala Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Thr Gly Ala Thr 1330 1335 1340 Gly Cys Ala Cys Thr Cys Ala Thr Cys Gly Ala Ala Gly Thr Thr Cys 1345 1350 1355 1360 Ala Ala Gly Gly Ala Ala Cys Cys Gly Cys Cys Ala Thr Thr Cys Ala 1365 1370 1375 Gly Cys Gly Thr Ala Thr Thr Cys Thr Thr Thr Ala Thr Thr Gly Thr 1380 1385 1390 Gly Ala Thr Gly Ala Thr Cys Cys Thr Gly Thr Thr Ala Gly Cys Cys 1395 1400 1405 Ala Ala Cys Thr Cys Ala Ala Gly Thr Gly Thr Thr Cys Thr Cys Ala 1410 1415 1420 Gly Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Ala Cys Cys Thr Thr 1425 1430 1435 1440 Gly Ala Cys Gly Ala Thr Gly Gly Thr Thr Thr Thr Thr Ala Cys Cys 1445 1450 1455 Cys Thr Ala Thr Thr Thr Cys Thr Thr Cys Thr Ala Gly Ala Ala Ala 1460 1465 1470 Cys Cys Thr Thr Cys Thr Gly Ala Gly Thr Cys Ala Thr Gly Ala Ala 1475 1480 1485 Cys Ala Gly Cys Cys Ala Ala Thr Thr Thr Cys Thr Thr Thr Thr Gly 1490 1495 1500 Thr Thr Ala Cys Thr Cys Thr Gly Cys Cys Ala Thr Cys Ala Thr Thr 1505 1510 1515 1520 Thr Ala Ala Thr Gly Ala Thr Cys Ala Thr Thr Cys Thr Thr Thr Thr 1525 1530 1535 Gly Thr Thr Ala Ala Cys Ala Thr Thr Ala Cys Thr Gly Thr Ala Thr 1540 1545 1550 Cys Thr Gly Cys Thr Thr Cys Cys Thr Thr Thr Gly Gly Thr Gly Gly 1555 1560 1565 Thr Cys Ala Thr Ala Gly Thr Gly Gly Thr Gly Cys Cys Ala Ala Cys 1570 1575 1580 Cys Thr Thr Ala Thr Thr Gly Cys Ala Thr Cys Thr Gly Ala Cys Ala 1585 1590 1595 1600 Cys Thr Ala Cys Thr Ala Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1605 1610 1615 Thr Ala Gly Thr Thr Cys Thr Thr Thr Cys Thr Gly Thr Gly Thr Thr 1620 1625 1630 Gly Ala Cys Ala Cys Thr Ala Gly Ala Cys Ala Ala Thr Thr Thr Ala 1635 1640 1645 Cys Cys Ala Thr Thr Thr Cys Ala Cys Thr Gly Thr Thr Thr Thr Ala 1650 1655 1660 Thr Ala Ala Cys Gly Thr Thr Ala Cys Ala Ala Ala Cys Ala Gly Thr 1665 1670 1675 1680 Thr Ala Thr Gly Gly Thr Thr Ala Thr Gly Thr Gly Thr Cys Thr Ala 1685 1690 1695 Ala Ala Thr Cys Ala Cys Ala Gly Gly Ala Cys Ala Gly Thr Ala Ala 1700 1705 1710 Thr Thr Gly Cys Cys Cys Thr Thr Thr Cys Ala Cys Cys Thr Thr Gly 1715 1720 1725 Cys Ala Ala Thr Cys Thr Gly Thr Thr Ala Ala Thr Gly Ala Thr Thr 1730 1735 1740 Ala Cys Cys Thr Gly Thr Cys Thr Thr Thr Thr Ala Gly Cys Ala Ala 1745 1750 1755 1760 Ala Thr Thr Thr Thr Gly Thr Gly Thr Thr Thr Cys Cys Ala Cys Cys 1765 1770 1775 Ala Gly Cys Cys Thr Thr Thr Thr Gly Gly Cys Thr Ala Gly Thr Gly 1780 1785 1790 Cys Cys Thr Gly Thr Ala Cys Cys Ala Thr Ala Gly Ala Thr Cys Thr 1795 1800 1805 Thr Thr Thr Thr Gly Gly Thr Thr Ala Cys Cys Cys Thr Gly Ala Gly 1810 1815 1820 Thr Thr Thr Gly Gly Thr Ala Gly Thr Gly Gly Thr Gly Thr Thr Ala 1825 1830 1835 1840 Ala Gly Thr Thr Thr Ala Cys Gly Thr Cys Cys Cys Thr Thr Thr Ala 1845 1850 1855 Cys Thr Thr Thr Cys Ala Ala Thr Thr Cys Ala Cys Ala Ala Ala Gly 1860 1865 1870 Gly Gly Thr Gly Ala Gly Thr Thr Gly Ala Thr Thr Ala Cys Thr Gly 1875 1880 1885 Gly Cys Ala Cys Gly Cys Cys Thr Ala Ala Ala Cys Cys Ala Cys Thr 1890 1895 1900 Thr Gly Ala Ala Gly Gly Thr Gly Thr Cys Ala Cys Gly Gly Ala Cys 1905 1910 1915 1920 Gly Thr Thr Thr Cys Thr Thr Thr Thr Ala Thr Gly Ala Cys Thr Cys 1925 1930 1935 Thr Gly Gly Ala Thr Gly Thr Gly Thr Gly Thr Ala Cys Cys Ala Ala 1940 1945 1950 Gly Thr Ala Thr Ala Cys Thr Ala Thr Cys Thr Ala Thr Gly Gly Cys 1955 1960 1965 Thr Thr Thr Ala Ala Ala Gly Gly Thr Gly Ala Gly Gly Gly Thr Ala 1970 1975 1980 Thr Cys Cys Thr Thr Ala Cys Cys Cys Thr Thr Ala Cys Ala Ala Ala 1985 1990 1995 2000 Thr Thr Cys Thr Ala Gly Cys Thr Thr Thr Thr Thr Gly Gly Cys Ala 2005 2010 2015 Gly Gly Thr Gly Thr Thr Thr Ala Thr Thr Ala Cys Ala Cys Ala Thr 2020 2025 2030 Cys Thr Gly Ala Thr Thr Cys Thr Gly Gly Ala Cys Ala Gly Thr Thr 2035 2040 2045 Gly Thr Thr Ala Gly Cys Cys Thr Thr Thr Ala Ala Gly Ala Ala Thr 2050 2055 2060 Gly Thr Cys Ala Cys Thr Ala Gly Thr Gly Gly Thr Gly Cys Thr Gly 2065 2070 2075 2080 Thr Thr Thr Ala Thr Thr Cys Thr Gly Thr Thr Ala Cys Gly Cys Cys 2085 2090 2095 Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Cys Ala Gly Ala Gly 2100 2105 2110 Cys Ala Gly Gly Cys Thr Gly Cys Ala Thr Ala Thr Gly Thr Thr Gly 2115 2120 2125 Ala Thr Gly Ala Thr Gly Ala Thr Ala Thr Ala Gly Thr Gly Gly Gly 2130 2135 2140 Thr Gly Thr Thr Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Thr Gly 2145 2150 2155 2160 Thr Cys Thr Ala Gly Cys Thr Cys Cys Ala Cys Thr Thr Thr Thr Ala 2165 2170 2175 Ala Cys Ala Gly Thr Ala Cys Thr Ala Gly Gly Gly Ala Gly Thr Thr 2180 2185 2190 Gly Cys Cys Thr Gly Gly Thr Thr Thr Cys Thr Thr Cys Thr Ala Cys 2195 2200 2205 Cys Ala Thr Thr Cys Thr Ala Ala Thr Gly Ala Thr Gly Gly Cys Thr 2210 2215 2220 Cys Thr Ala Ala Thr Thr Gly Thr Ala Cys Ala Gly Ala Gly Cys Cys 2225 2230 2235 2240 Thr Gly Thr Gly Thr Thr Gly Gly Thr Gly Thr Ala Thr Ala Gly Thr 2245 2250 2255 Ala Ala Cys Ala Thr Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala 2260 2265 2270 Ala Ala Thr Cys Thr Gly Gly Cys Ala Gly Thr Ala Thr Thr Gly Gly 2275 2280 2285 Cys Thr Ala Cys Gly Thr Cys Cys Cys Ala Thr Cys Thr Cys Ala Gly 2290 2295 2300 Thr Cys Thr Gly Gly Cys Cys Ala Ala Gly Thr Cys Ala Ala Gly Ala 2305 2310 2315 2320 Thr Thr Gly Cys Ala Cys Cys Cys Ala Cys Gly Gly Thr Thr Ala Cys 2325 2330 2335 Thr Gly Gly Gly Ala Ala Thr Ala Thr Thr Ala Gly Thr Ala Thr Thr 2340 2345 2350 Cys Cys Cys Ala Cys Cys Ala Ala Cys Thr Thr Thr Ala Gly Thr Ala 2355 2360 2365 Thr Gly Ala Gly Thr Ala Thr Thr Ala Gly Gly Ala Cys Ala Gly Ala 2370 2375 2380 Ala Thr Ala Thr Thr Thr Ala Cys Ala Gly Cys Thr Thr Thr Ala Cys 2385 2390 2395 2400 Ala Ala Cys Ala Cys Gly Cys Cys Thr Gly Thr Thr Ala Gly Thr Gly 2405 2410 2415 Thr Thr Gly Ala Thr Thr Gly Thr Gly Cys Cys Ala Cys Ala Thr Ala 2420 2425 2430 Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Thr Ala Ala Cys 2435 2440 2445 Thr Cys Thr Cys Gly Thr Thr Gly Thr Ala Ala Ala Cys Ala Ala Thr 2450 2455 2460 Thr Ala Cys Thr Cys Ala Cys Cys Cys Ala Gly Thr Ala Cys Ala Cys 2465 2470 2475 2480 Thr Gly Cys Ala Gly Cys Ala Thr Gly Thr Ala Ala Gly Ala Cys Cys 2485 2490 2495 Ala Thr Ala Gly Ala Gly Thr Cys Ala Gly Cys Ala Thr Thr Ala Cys 2500 2505 2510 Ala Ala Cys Thr Cys Ala Gly Cys Gly Cys Thr Ala Gly Gly Cys Thr 2515 2520 2525 Thr Gly Ala Gly Thr Cys Thr Gly Thr Thr Gly Ala Ala Gly Thr Thr 2530 2535 2540 Ala Ala Cys Thr Cys Thr Ala Thr Gly Cys Thr Thr Ala Cys Thr Ala 2545 2550 2555 2560 Thr Thr Thr Cys Thr Gly Ala Ala Gly Ala Gly Gly Cys Thr Cys Thr 2565 2570 2575 Ala Cys Ala Gly Thr Thr Ala Gly Cys Thr Ala Cys Cys Ala Thr Thr 2580 2585 2590 Ala Gly Thr Thr Cys Gly Thr Thr Thr Ala Ala Thr Gly Gly Thr Gly 2595 2600 2605 Ala Thr Gly Gly Ala Thr Ala Thr Ala Ala Thr Thr Thr Thr Ala Cys 2610 2615 2620 Thr Ala Ala Thr Gly Thr Gly Cys Thr Gly Gly Gly Thr Gly Thr Thr 2625 2630 2635 2640 Thr Cys Thr Gly Thr Gly Thr Ala Thr Gly Ala Thr Cys Cys Thr Gly 2645 2650 2655 Cys Ala Ala Gly Thr Gly Gly Cys Ala Gly Gly Gly Thr Gly Gly Thr 2660 2665 2670 Ala Cys Ala Ala Ala Ala Ala Ala Gly Gly Thr Cys Thr Thr Thr Thr 2675 2680 2685 Ala Thr Thr Gly Ala Ala Gly Ala Cys Cys Thr Gly Cys Thr Thr Thr 2690 2695 2700 Thr Thr Ala Ala Thr Ala Ala Ala Gly Thr Gly Gly Thr Thr Ala Cys 2705 2710 2715 2720 Thr Ala Ala Thr Gly Gly Cys Cys Thr Thr Gly Gly Thr Ala Cys Thr 2725 2730 2735 Gly Thr Thr Gly Ala Thr Gly Ala Ala Gly Ala Cys Thr Ala Thr Ala 2740 2745 2750 Ala Gly Cys Gly Cys Thr Gly Thr Thr Cys Thr Ala Ala Thr Gly Gly 2755 2760 2765 Thr Cys Gly Cys Thr Cys Thr Gly Thr Gly Gly Cys Ala Gly Ala Thr 2770 2775 2780 Cys Thr Ala Gly Thr Cys Thr Gly Thr Gly Cys Ala Cys Ala Gly Thr 2785 2790 2795 2800 Ala Thr Thr Ala Cys Thr Cys Thr Gly Gly Thr Gly Thr Cys Ala Thr 2805 2810 2815 Gly Gly Thr Ala Cys Thr Ala Cys Cys Thr Gly Gly Thr Gly Thr Thr 2820 2825 2830 Gly Thr Thr Gly Ala Cys Gly Cys Thr Gly Ala Gly Ala Ala Gly Cys 2835 2840 2845 Thr Thr Cys Ala Cys Ala Thr Gly Thr Ala Thr Ala Gly Thr Gly Cys 2850 2855 2860 Gly Thr Cys Thr Cys Thr Cys Ala Thr Cys Gly Gly Thr Gly Gly Thr 2865 2870 2875 2880 Ala Thr Gly Gly Thr Gly Cys Thr Ala Gly Gly Ala Gly Gly Thr Thr 2885 2890 2895 Thr Thr Ala Cys Thr Thr Cys Thr Gly Cys Ala Gly Cys Gly Gly Cys 2900 2905 2910 Ala Thr Thr Gly Cys Cys Thr Thr Thr Thr Ala Gly Cys Thr Ala Thr 2915 2920 2925 Gly Cys Thr Gly Thr Thr Cys Ala Ala Gly Cys Thr Ala Gly Ala Cys 2930 2935 2940 Thr Cys Ala Ala Thr Thr Ala Thr Cys Thr Thr Gly Cys Thr Cys Thr 2945 2950 2955 2960 Ala Cys Ala Gly Ala Cys Gly Gly Ala Thr Gly Thr Thr Cys Thr Ala 2965 2970 2975 Cys Ala Gly Cys Gly Gly Ala Ala Cys Cys Ala Gly Cys Ala Ala Thr 2980 2985 2990 Thr Gly Cys Thr Thr Gly Cys Thr Gly Ala Gly Thr Cys Thr Thr Thr 2995 3000 3005 Thr Ala Ala Cys Thr Cys Thr Gly Cys Thr Ala Thr Thr Gly Gly Thr 3010 3015 3020 Ala Ala Thr Ala Thr Ala Ala Cys Thr Thr Cys Ala Gly Cys Cys Thr 3025 3030 3035 3040 Thr Thr Gly Ala Gly Ala Gly Thr Gly Thr Thr Ala Ala Ala Gly Ala 3045 3050 3055 Gly Gly Cys Thr Ala Thr Thr Ala Gly Thr Cys Ala Ala Ala Cys Thr 3060 3065 3070 Thr Cys Cys Ala Ala Gly Gly Gly Thr Thr Thr Gly Ala Ala Cys Ala 3075 3080 3085 Cys Thr Gly Thr Gly Gly Cys Thr Cys Ala Thr Gly Cys Gly Cys Thr 3090 3095 3100 Thr Ala Cys Thr Ala Ala Gly Gly Thr Thr Cys Ala Ala Gly Ala Gly 3105 3110 3115 3120 Gly Thr Thr Gly Thr Thr Ala Ala Cys Thr Cys Gly Cys Ala Gly Gly 3125 3130 3135 Gly Thr Gly Cys Ala Gly Cys Thr Thr Thr Gly Ala Cys Thr Cys Ala 3140 3145 3150 Ala Cys Thr Thr Ala Cys Cys Gly Thr Ala Cys Ala Gly Cys Thr Gly 3155 3160 3165 Cys Ala Ala Cys Ala Cys Ala Ala Cys Thr Thr Cys Cys Ala Ala Gly 3170 3175 3180 Cys Cys Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Cys Thr Ala Thr 3185 3190 3195 3200 Thr Gly Ala Thr Gly Ala Cys Ala Thr Thr Thr Ala Cys Thr Cys Thr 3205 3210 3215 Cys Gly Ala Cys Thr Gly Gly Ala Cys Ala Thr Thr Cys Thr Thr Thr 3220 3225 3230 Cys Ala Gly Cys Cys Gly Ala Thr Gly Thr Thr Cys Ala Gly Gly Thr 3235 3240 3245 Thr Gly Ala Cys Cys Gly Thr Cys Thr Cys Ala Thr Cys Ala Cys Cys 3250 3255 3260 Gly Gly Cys Ala Gly Ala Thr Thr Ala Thr Cys 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6670 Gly Thr Cys Gly Ala Ala Ala Ala Ala Gly Gly Thr Gly Thr Thr Gly 6675 6680 6685 Ala Thr Gly Cys Cys Thr Cys Ala Gly Gly Cys Thr Ala Thr Gly Cys 6690 6695 6700 Thr Cys Ala Gly Ala Thr Cys Gly Cys Cys Ala Gly Thr Thr Thr Ala 6705 6710 6715 6720 Gly Cys Ala Cys Cys Ala Ala Ala Thr Gly Thr Thr Gly Cys Ala Gly 6725 6730 6735 Cys Ala Thr Thr Gly Cys Thr Cys Thr Thr Thr Gly Gly Thr Gly Gly 6740 6745 6750 Thr Ala Ala Thr Gly Thr Gly Gly Cys Thr Gly Thr Thr Cys Gly Thr 6755 6760 6765 Gly Ala Gly Cys Thr Ala Gly Cys Gly Gly Ala Cys Thr Cys Thr Thr 6770 6775 6780 Ala Cys Gly Ala Gly Ala Thr Thr Ala Cys Ala Thr Ala Thr Ala Ala 6785 6790 6795 6800 Thr Thr Ala Thr Ala Ala Ala Ala Thr Gly Ala Cys Thr Gly Thr Gly 6805 6810 6815 Cys Cys Ala Ala Ala Gly Thr Cys Thr Gly Ala Thr Cys Cys Ala Ala 6820 6825 6830 Ala Thr Gly Thr Ala Gly Ala Gly Cys Thr Thr Cys Thr Thr Gly Thr 6835 6840 6845 Thr Thr Cys Ala Cys Ala Gly Gly Thr Gly Gly Ala Thr Gly Cys Ala 6850 6855 6860 Thr Thr Thr Ala Ala Ala Ala Cys Thr Gly Gly Gly Ala Ala Thr Gly 6865 6870 6875 6880 Cys Ala Ala Ala Ala Cys Cys Cys Cys Ala Gly Ala Gly Ala Ala Ala 6885 6890 6895 Gly Ala Ala Gly Gly Ala Ala Ala Ala Gly Ala Ala Gly Ala Ala Cys 6900 6905 6910 Ala Ala Gly Cys Gly Thr Gly Ala Ala Ala Cys Cys Ala Cys Gly Cys 6915 6920 6925 Ala Gly Cys Ala Gly Cys Thr Gly Ala Ala Thr Gly Ala Ala Gly Ala 6930 6935 6940 Gly Gly Cys Cys Ala Thr Cys Thr Ala Cys Gly Ala Thr Gly Ala Thr 6945 6950 6955 6960 Gly Thr Gly Gly Gly Thr Gly Thr Gly Cys Cys Ala Thr Cys Thr Gly 6965 6970 6975 Ala Thr Gly Thr Gly Ala Cys Thr Cys Ala Thr Gly Cys Cys Ala Ala 6980 6985 6990 Thr Thr Thr Gly Gly Ala Ala Thr Gly Gly Gly Ala Cys Ala Cys Ala 6995 7000 7005 Gly Cys Thr Gly Thr Thr Gly Ala Thr Gly Gly Thr Gly Gly Thr Gly 7010 7015 7020 Ala Cys Ala Cys Gly Gly Cys Cys Gly Thr Thr Gly Ala Ala Ala Thr 7025 7030 7035 7040 Thr Ala Thr Cys Ala Ala Cys Gly Ala Gly Ala Thr Cys Thr Thr Cys 7045 7050 7055 Gly Ala Cys Ala Cys Ala Gly Gly Ala Ala Ala Thr Thr Ala Ala Ala 7060 7065 7070 Cys Ala Ala Thr Gly Thr Thr Thr Gly Ala Cys Thr Gly Gly Cys Thr 7075 7080 7085 Thr Ala Thr Cys Cys Thr Gly Gly Cys Thr Ala Thr Gly Thr Cys Cys 7090 7095 7100 Cys Ala Gly Gly Gly Thr Ala Gly Thr Gly Cys Cys Ala Thr Thr Ala 7105 7110 7115 7120 Cys Ala Cys Thr Gly Thr Thr Ala Thr Thr Ala Cys Thr Gly Ala Gly 7125 7130 7135 Thr Gly Thr Thr Thr Thr Thr Cys Thr Ala Gly Cys Gly Ala Cys Thr 7140 7145 7150 Thr Gly Gly Cys Thr Gly Cys Thr Gly Gly Gly Cys Thr Ala Thr Gly 7155 7160 7165 Gly Cys Thr Thr Thr Gly Cys Cys Cys Thr Cys Thr Ala Ala Cys Thr 7170 7175 7180 Ala Gly Cys Gly Gly Thr Cys Thr Thr Gly Gly Thr Cys Thr Thr Gly 7185 7190 7195 7200 Cys Ala Cys Ala Cys Ala Ala Cys Gly Gly Thr Ala Ala Gly Cys Cys 7205 7210 7215 Ala Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Cys Ala Gly Thr Gly 7220 7225 7230 Cys Ala Ala Gly Ala Ala Gly Gly Ala Thr Ala Thr Thr Ala Cys Cys 7235 7240 7245 Ala Thr Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Thr Gly Ala Gly 7250 7255 7260 Gly Gly Gly Ala Ala Cys Gly Cys Ala Gly Thr Ala Cys Cys Thr Thr 7265 7270 7275 7280 Thr Thr Cys Ala Thr Cys Thr Ala Ala Ala Cys Cys Thr Thr Thr Gly 7285 7290 7295 Cys Ala Cys Gly Ala Gly Thr Ala Ala Thr Cys Ala Ala Ala Gly Ala 7300 7305 7310 Thr Cys Cys Gly Cys Thr Thr Gly Ala Cys Gly Ala Gly Cys Cys Thr 7315 7320 7325 Ala Thr Ala Thr Gly Gly Ala Ala Gly Ala Gly Cys Gly Thr Gly Cys 7330 7335 7340 Cys Ala Gly Gly Thr Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 7345 7350 7355 7360 Gly Gly Ala Cys Thr Gly Thr Thr Ala Gly Thr Ala Ala Cys Thr Gly 7365 7370 7375 Ala Ala Gly Ala Cys Cys Thr Gly Ala Cys Gly Gly Thr Gly Thr Thr 7380 7385 7390 Gly Ala Thr Ala Thr Gly Gly Ala Thr Ala Cys Ala Cys 7395 7400 7405 <210> 40 <211> 1386 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 spike protein <400> 40 Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 1 5 10 15 Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 20 25 30 Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 35 40 45 Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 50 55 60 Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 65 70 75 80 Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 85 90 95 Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 100 105 110 Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 115 120 125 Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 130 135 140 Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 145 150 155 160 His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 165 170 175 Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 180 185 190 Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 195 200 205 Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 210 215 220 Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 225 230 235 240 Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 245 250 255 Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Thr 260 265 270 Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 275 280 285 Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 290 295 300 Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 305 310 315 320 Phe Asn Ile Asn Asp Thr Ser Val Ile Leu Ala Glu Gly Ser Ile Val 325 330 335 Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 340 345 350 Ser Asn Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Thr Gln 355 360 365 Val Pro Tyr Tyr Cys Phe Phe Lys Val Asp Thr Tyr Asn Ser Thr Val 370 375 380 Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 385 390 395 400 Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 405 410 415 Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 420 425 430 Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 435 440 445 Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 450 455 460 Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 465 470 475 480 Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 485 490 495 Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 500 505 510 Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 515 520 525 Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 530 535 540 Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 545 550 555 560 Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 565 570 575 Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 580 585 590 Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 595 600 605 Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 610 615 620 Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 625 630 635 640 Val Ser Phe Met Thr Leu Asp Val Cys Thr Lys Tyr Thr Ile Tyr Gly 645 650 655 Phe Lys Gly Glu Gly Ile Leu Thr Leu Thr Asn Ser Ser Phe Leu Ala 660 665 670 Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 675 680 685 Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 690 695 700 Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 705 710 715 720 Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 725 730 735 His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 740 745 750 Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 755 760 765 Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 770 775 780 Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 785 790 795 800 Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 805 810 815 Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 820 825 830 Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 835 840 845 Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 850 855 860 Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 865 870 875 880 Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 885 890 895 Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 900 905 910 Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 915 920 925 Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 930 935 940 Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 945 950 955 960 Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 965 970 975 Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 980 985 990 Gln Arg Asn Gln Gln Leu Leu Ala Glu Ser Phe Asn Ser Ala Ile Gly 995 1000 1005 Asn Ile Thr Ser Ala Phe Glu Ser Val Lys Glu Ala Ile Ser Gln Thr 1010 1015 1020 Ser Lys Gly Leu Asn Thr Val Ala His Ala Leu Thr Lys Val Gln Glu 1025 1030 1035 1040 Val Val Asn Ser Gln Gly Ala Ala Leu Thr Gln Leu Thr Val Gln Leu 1045 1050 1055 Gln His Asn Phe Gln Ala Ile Ser Ser Ser Ile Asp Asp Ile Tyr Ser 1060 1065 1070 Arg Leu Asp Ile Leu Ser Ala Asp Val Gln Val Asp Arg Leu Ile Thr 1075 1080 1085 Gly Arg Leu Ser Ala Leu Asn Ala Phe Val Ala Gln Thr Leu Thr Lys 1090 1095 1100 Tyr Thr Glu Val Gln Ala Ser Arg Lys Leu Ala Gln Gln Lys Val Asn 1105 1110 1115 1120 Glu Cys Val Lys Ser Gln Ser Gln Arg Tyr Gly Phe Cys Gly Gly Asp 1125 1130 1135 Gly Glu His Ile Phe Ser Leu Val Gln Ala Ala Pro Gln Gly Leu Leu 1140 1145 1150 Phe Leu His Thr Val Leu Val Pro Ser Asp Phe Val Asp Val Ile Ala 1155 1160 1165 Ile Ala Gly Leu Cys Val Asn Asp Glu Ile Ala Leu Thr Leu Arg Glu 1170 1175 1180 Pro Gly Leu Val Leu Phe Thr His Glu Leu Gln Asn His Thr Ala Met 1185 1190 1195 1200 Glu Tyr Phe Val Ser Ser Arg Arg Met Phe Glu Pro Arg Lys Pro Thr 1205 1210 1215 Val Ser Asp Phe Val Gln Ile Glu Ser Cys Val Val Thr Tyr Val Asn 1220 1225 1230 Leu Thr Arg Asp Gln Leu Pro Asp Val Ile Pro Asp Tyr Ile Asp Val 1235 1240 1245 Asn Lys Thr Leu Asp Glu Ile Leu Ala Ser Leu Pro Asn Arg Thr Gly 1250 1255 1260 Pro Ser Leu Pro Leu Asp Val Phe Asn Ala Thr Tyr Leu Asn Leu Thr 1265 1270 1275 1280 Gly Glu Ile Ala Asp Leu Gln Gln Arg Ser Glu Ser Leu Arg Asn Thr 1285 1290 1295 Thr Glu Glu Leu Gln Ser Leu Ile Tyr Asn Ile Asn Asn Thr Leu Val 1300 1305 1310 Asp Leu Glu Trp Leu Asn Arg Val Glu Thr Tyr Ile Lys Trp Pro Trp 1315 1320 1325 Trp Val Trp Leu Ile Ile Phe Ile Val Leu Ile Phe Val Val Ser Leu 1330 1335 1340 Leu Val Phe Cys Cys Ile Ser Thr Gly Cys Cys Gly Cys Cys Gly Cys 1345 1350 1355 1360 Cys Cys Ala Cys Phe Ser Gly Cys Cys Arg Gly Pro Arg Leu Gln Pro 1365 1370 1375 Tyr Glu Val Phe Glu Lys Val His Val Gln 1380 1385 <210> 41 <211> 224 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 ORF3 <400> 41 Met Phe Leu Gly Leu Phe Gln Tyr Thr Ile Asp Thr Val Val Lys Asp 1 5 10 15 Val Ser Lys Ser Ala Asn Leu Ser Leu Tyr Ala Val Gln Glu Leu Glu 20 25 30 Leu Asn Val Val Pro Ile Arg Gln Ala Ser Asn Val Thr Gly Phe Leu 35 40 45 Phe Thr Ser Val Phe Ile Tyr Phe Phe Ala Leu Phe Lys Ala Ser Ser 50 55 60 Leu Arg Arg Asn Tyr Ile Met Leu Ala Ala Arg Phe Ala Val Ile Val 65 70 75 80 Leu Tyr Cys Pro Leu Leu Tyr Tyr Cys Gly Ala Phe Leu Asp Ala Thr 85 90 95 Ile Ile Cys Cys Thr Leu Ile Gly Arg Leu Cys Leu Val Cys Phe Tyr 100 105 110 Ser Trp Arg Tyr Lys Asn Ala Leu Phe Ile Ile Phe Asn Thr Thr Thr 115 120 125 Leu Ser Phe Leu Asn Gly Lys Ala Ala Tyr Tyr Asp Gly Lys Ser Ile 130 135 140 Val Ile Leu Glu Gly Gly Asp His Tyr Ile Thr Phe Gly Asn Ser Phe 145 150 155 160 Val Ala Phe Val Ser Ser Ile Asp Leu Tyr Leu Ala Ile Arg Gly Arg 165 170 175 Gln Glu Ala Asp Leu Gln Leu Leu Arg Thr Val Glu Leu Leu Asp Gly 180 185 190 Lys Lys Leu Tyr Val Phe Ser Gln His Gln Ile Val Gly Ile Thr Asn 195 200 205 Ala Ala Phe Asp Ser Ile Gln Leu Asp Glu Tyr Ala Thr Ile Ser Glu 210 215 220 <210> 42 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 envelope protein <400> 42 Met Leu Gln Leu Val Asn Asp Asn Gly Leu Val Val Asn Val Ile Leu 1 5 10 15 Trp Leu Phe Val Leu Phe Phe Leu Leu Ile Ile Ser Ile Thr Phe Val 20 25 30 Gln Leu Val Asn Leu Cys Phe Thr Cys His Arg Leu Cys Asn Ser Ala 35 40 45 Val Tyr Thr Pro Ile Gly Arg Leu Tyr Arg Val Tyr Lys Ser Tyr Met 50 55 60 Gln Ile Asp Pro Leu Ser Ser Thr Val Ile Asp Val 65 70 75 <210> 43 <211> 226 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 membrane protein <400> 43 Met Ser Asn Gly Ser Ile Pro Val Asp Glu Val Ile Gln His Leu Arg 1 5 10 15 Asn Trp Asn Phe Thr Trp Asn Ile Ile Leu Thr Ile Leu Leu Val Val 20 25 30 Leu Gln Tyr Gly His Tyr Lys Tyr Ser Ala Phe Leu Tyr Gly Val Lys 35 40 45 Met Ala Ile Leu Trp Ile Leu Trp Pro Leu Val Leu Ala Leu Ser Leu 50 55 60 Phe Asp Ala Trp Ala Ser Phe Gln Val Asn Trp Val Phe Phe Ala Phe 65 70 75 80 Ser Ile Leu Met Ala Cys Ile Thr Leu Met Leu Trp Ile Met Tyr Phe 85 90 95 Val Asn Ser Ile Arg Leu Trp Arg Arg Thr His Ser Trp Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asp Ala Leu Leu Thr Thr Ser Val Met Gly Arg Gln 115 120 125 Val Cys Ile Pro Val Leu Gly Ala Pro Thr Gly Val Thr Leu Thr Leu 130 135 140 Leu Ser Gly Thr Leu Leu Val Glu Gly Tyr Lys Val Ala Thr Gly Val 145 150 155 160 Gln Val Ser Gln Leu Pro Asn Phe Val Thr Val Ala Lys Ala Thr Thr 165 170 175 Thr Ile Val Tyr Gly Arg Val Gly Arg Ser Val Asn Ala Ser Ser Gly 180 185 190 Thr Gly Trp Ala Phe Tyr Val Arg Ser Lys His Gly Asp Tyr Ser Ala 195 200 205 Val Ser Asn Pro Ser Ser Val Leu Thr Asp Ser Glu Lys Val Leu His 210 215 220 Leu Val 225 <210> 44 <211> 441 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 nucleocapsid protein <400> 44 Met Ala Ser Val Ser Phe Gln Asp Arg Gly Arg Lys Arg Val Pro Leu 1 5 10 15 Ser Leu Tyr Ala Pro Leu Arg Val Thr Asn Asp Lys Pro Leu Ser Lys 20 25 30 Val Leu Ala Asn Asn Ala Val Pro Thr Asn Lys Gly Asn Lys Asp Gln 35 40 45 Gln Ile Gly Tyr Trp Asn Glu Gln Ile Arg Trp Arg Met Arg Arg Gly 50 55 60 Glu Arg Ile Glu Gln Pro Ser Asn Trp His Phe Tyr Tyr Leu Gly Thr 65 70 75 80 Gly Pro His Ala Asp Leu Arg Tyr Arg Thr Arg Thr Glu Gly Val Phe 85 90 95 Trp Val Ala Lys Glu Gly Ala Lys Thr Glu Pro Thr Asn Leu Gly Val 100 105 110 Arg Lys Ala Ser Glu Lys Pro Ile Ile Pro Asn Phe Ser Gln Gln Leu 115 120 125 Pro Ser Val Val Glu Ile Val Glu Pro Asn Thr Pro Pro Thr Ser Arg 130 135 140 Ala Asn Ser Arg Ser Arg Ser Arg Gly Asn Gly Asn Asn Arg Ser Arg 145 150 155 160 Ser Pro Ser Asn Asn Arg Gly Asn Asn Gln Ser Arg Gly Asn Ser Gln 165 170 175 Asn Arg Gly Asn Asn Gln Gly Arg Gly Ala Ser Gln Asn Arg Gly Gly 180 185 190 Asn Asn Asn Asn Asn Asn Lys Ser Arg Asn Gln Ser Lys Asn Arg Asn 195 200 205 Gln Ser Asn Asp Arg Gly Gly Val Thr Ser Arg Asp Asp Leu Val Ala 210 215 220 Ala Val Lys Asp Ala Leu Lys Ser Leu Gly Ile Gly Glu Asn Pro Asp 225 230 235 240 Lys Leu Lys Gln Gln Gln Lys Pro Lys Gln Glu Arg Ser Asp Ser Ser 245 250 255 Gly Lys Asn Thr Pro Lys Lys Asn Lys Ser Arg Ala Thr Ser Lys Glu 260 265 270 Arg Asp Leu Lys Asp Ile Pro Glu Trp Arg Arg Ile Pro Lys Gly Glu 275 280 285 Asn Ser Val Ala Ala Cys Phe Gly Pro Arg Gly Gly Phe Lys Asn Phe 290 295 300 Gly Asp Ala Glu Phe Val Glu Lys Gly Val Asp Ala Ser Gly Tyr Ala 305 310 315 320 Gln Ile Ala Ser Leu Ala Pro Asn Val Ala Ala Leu Leu Phe Gly Gly 325 330 335 Asn Val Ala Val Arg Glu Leu Ala Asp Ser Tyr Glu Ile Thr Tyr Asn 340 345 350 Tyr Lys Met Thr Val Pro Lys Ser Asp Pro Asn Val Glu Leu Leu Val 355 360 365 Ser Gln Val Asp Ala Phe Lys Thr Gly Asn Ala Lys Pro Gln Arg Lys 370 375 380 Lys Glu Lys Lys Asn Lys Arg Glu Thr Thr Gln Gln Leu Asn Glu Glu 385 390 395 400 Ala Ile Tyr Asp Asp Val Gly Val Pro Ser Asp Val Thr His Ala Asn 405 410 415 Leu Glu Trp Asp Thr Ala Val Asp Gly Gly Asp Thr Ala Val Glu Ile 420 425 430 Ile Asn Glu Ile Phe Asp Thr Gly Asn 435 440 <210> 45 <211> 7405 <212> PRT <213> Artificial Sequence <220> <223> KNU-141112-P30 complete cds <400> 45 Ala Thr Gly Ala Ala Gly Thr Cys Thr Thr Thr Ala Ala Cys Cys Thr 1 5 10 15 Ala Cys Thr Thr Cys Thr Gly Gly Thr Thr Gly Thr Thr Cys Thr Thr 20 25 30 Ala Cys Cys Ala Gly Thr Ala Cys Thr Thr Thr Cys Ala Ala Cys Ala 35 40 45 Cys Thr Thr Ala Gly Cys Cys Thr Ala Cys Cys Ala Cys Ala Ala Gly 50 55 60 Ala Thr Gly Thr Cys Ala Cys Cys Ala Gly Gly Thr Gly Cys Thr Cys 65 70 75 80 Ala Gly Cys Thr Ala Ala Cys Ala Cys Thr Ala Ala Thr Thr Thr Thr 85 90 95 Ala Gly Gly Cys Gly Gly Thr Thr Cys Thr Thr Thr Thr Cys Ala Ala 100 105 110 Ala Ala Thr Thr Thr Ala Ala Thr Gly Thr Thr Cys Ala Gly Gly Cys 115 120 125 Gly Cys Cys Thr Gly Cys Ala Gly Thr Thr Gly Thr Thr Gly Thr Ala 130 135 140 Cys Thr Gly Gly Gly Cys Gly Gly Thr Thr Ala Thr Cys Thr Ala Cys 145 150 155 160 Cys Thr Ala Thr Thr Gly Gly Thr Gly Ala Ala Ala Ala Cys Cys Ala 165 170 175 Gly Gly Gly Thr Gly Thr Cys Ala Ala Thr Thr Cys Ala Ala Cys Thr 180 185 190 Thr Gly Gly Thr Ala Cys Thr Gly Thr Gly Cys Thr Gly Gly Cys Cys 195 200 205 Ala Ala Cys Ala Thr Cys Cys Ala Ala Cys Thr Gly Cys Thr Ala Gly 210 215 220 Thr Gly Gly Cys Gly Thr Thr Cys Ala Thr Gly Gly Thr Ala Thr Cys 225 230 235 240 Thr Thr Thr Gly Thr Thr Ala Gly Cys Cys Ala Thr Ala Thr Thr Ala 245 250 255 Gly Ala Gly Gly Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Thr Thr 260 265 270 Thr Gly Ala Gly Ala Thr Thr Gly Gly Cys Ala Thr Thr Thr Cys Gly 275 280 285 Cys Ala Ala Gly Ala Gly Cys Cys Thr Thr Thr Thr Gly Ala Cys Cys 290 295 300 Cys Thr Ala Gly Thr Gly Gly Thr Thr Ala Cys Cys Ala Gly Cys Thr 305 310 315 320 Thr Thr Ala Thr Thr Thr Ala Cys Ala Thr Ala Ala Gly Gly Cys Thr 325 330 335 Ala Cys Thr Ala Ala Cys Gly Gly Thr Ala Ala Cys Ala Cys Thr Ala 340 345 350 Ala Thr Gly Cys Thr Ala Cys Thr Gly Cys Gly Cys Gly Ala Cys Thr 355 360 365 Gly Cys Gly Cys Ala Thr Thr Thr Gly Cys Cys Ala Gly Thr Thr Thr 370 375 380 Cys Cys Thr Ala Gly Cys Ala Thr Thr Ala Ala Ala Ala Cys Ala Thr 385 390 395 400 Thr Gly Gly Gly Cys Cys Cys Cys Ala Cys Thr Gly Cys Thr Ala Ala 405 410 415 Thr Ala Ala Thr Gly Ala Thr Gly Thr Thr Ala Cys Ala Ala Cys Ala 420 425 430 Gly Gly Thr Cys Gly Thr Ala Ala Thr Thr Gly Cys Cys Thr Ala Thr 435 440 445 Thr Thr Ala Ala Cys Ala Ala Ala Gly Cys Cys Ala Thr Cys Cys Cys 450 455 460 Ala Gly Cys Thr Cys Ala Thr Ala Thr Gly Ala Gly Thr Gly Ala Ala 465 470 475 480 Cys Ala Thr Ala Gly Thr Gly Thr Thr Gly Thr Cys Gly Gly Cys Ala 485 490 495 Thr Ala Ala Cys Ala Thr Gly Gly Gly Ala Thr Ala Ala Thr Gly Ala 500 505 510 Thr Cys Gly Thr Gly Thr Cys Ala Cys Thr Gly Thr Cys Thr Thr Thr 515 520 525 Thr Cys Thr Gly Ala Cys Ala Ala Gly Ala Thr Cys Thr Ala Thr Thr 530 535 540 Ala Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Ala Ala Ala Ala Ala 545 550 555 560 Thr Gly Ala Thr Thr Gly Gly Thr Cys Cys Cys Gly Thr Gly Thr Thr 565 570 575 Gly Cys Gly Ala Cys Ala Ala Ala Gly Thr Gly Thr Thr Ala Cys Ala 580 585 590 Ala Cys Ala Gly Thr Gly Gly Ala Gly Gly Thr Thr Gly Thr Gly Cys 595 600 605 Thr Ala Thr Gly Cys Ala Ala Thr Ala Thr Gly Thr Thr Thr Ala Cys 610 615 620 Gly Ala Ala Cys Cys Cys Ala Cys Cys Thr Ala Thr Thr Ala Cys Ala 625 630 635 640 Thr Gly Cys Thr Thr Ala Ala Thr Gly Thr Thr Ala Cys Thr Ala Gly 645 650 655 Thr Gly Cys Thr Gly Gly Thr Gly Ala Gly Gly Ala Thr Gly Gly Thr 660 665 670 Ala Thr Thr Thr Cys Thr Thr Ala Thr Cys Ala Ala Cys Cys Cys Thr 675 680 685 Gly Thr Ala Cys Ala Gly Cys Thr Ala Ala Thr Thr Gly Cys Ala Thr 690 695 700 Thr Gly Gly Thr Thr Ala Thr Gly Cys Thr Gly Cys Cys Ala Ala Thr 705 710 715 720 Gly Thr Ala Thr Thr Thr Gly Cys Thr Ala Cys Thr Gly Ala Gly Cys 725 730 735 Cys Cys Ala Ala Thr Gly Gly Cys Cys Ala Cys Ala Thr Ala Cys Cys 740 745 750 Ala Gly Ala Ala Gly Gly Thr Thr Thr Thr Ala Gly Thr Thr Thr Thr 755 760 765 Ala Ala Thr Ala Ala Thr Thr Gly Gly Thr Thr Thr Cys Thr Thr Thr 770 775 780 Thr Gly Thr Cys Cys Ala Ala Thr Gly Ala Thr Thr Cys Cys Ala Cys 785 790 795 800 Thr Thr Thr Gly Gly Thr Gly Cys Ala Thr Gly Gly Thr Ala Cys Gly 805 810 815 Gly Thr Gly Gly Thr Thr Thr Cys Cys Ala Ala Cys Cys Ala Ala Cys 820 825 830 Cys Ala Thr Thr Gly Thr Thr Gly Gly Thr Cys Ala Ala Thr Thr Gly 835 840 845 Thr Cys Thr Thr Thr Thr Gly Gly Cys Cys Ala Thr Thr Cys Cys Thr 850 855 860 Ala Ala Gly Ala Thr Thr Thr Ala Thr Gly Gly Ala Cys Thr Ala Gly 865 870 875 880 Gly Cys Cys Ala Ala Thr Thr Thr Thr Thr Cys Thr Cys Cys Thr Thr 885 890 895 Thr Ala Ala Thr Cys Ala Ala Ala Cys Gly Ala Thr Cys Gly Ala Thr 900 905 910 Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala Ala Thr Gly Gly Ala Gly 915 920 925 Cys Thr Gly Cys Thr Gly Thr Gly Cys Ala Gly Cys Gly Thr Gly Cys 930 935 940 Ala Cys Cys Ala Gly Ala Gly Gly Cys Thr Cys Thr Gly Ala Gly Gly 945 950 955 960 Thr Thr Thr Ala Ala Thr Ala Thr Thr Ala Ala Thr Gly Ala Cys Ala 965 970 975 Cys Cys Thr Cys Thr Gly Thr Cys Ala Thr Thr Cys Thr Thr Gly Cys 980 985 990 Thr Gly Ala Ala Gly Gly Cys Thr Cys Ala Ala Thr Thr Gly Thr Ala 995 1000 1005 Cys Thr Thr Cys Ala Thr Ala Cys Thr Gly Cys Thr Thr Thr Ala Gly 1010 1015 1020 Gly Ala Ala Cys Ala Ala Ala Thr Thr Thr Thr Thr Cys Thr Thr Thr 1025 1030 1035 1040 Thr Gly Thr Thr Thr Gly Cys Ala Gly Thr Ala Ala Thr Thr Cys Cys 1045 1050 1055 Thr Cys Ala Ala Ala Thr Cys Cys Thr Cys Ala Cys Thr Thr Ala Gly 1060 1065 1070 Cys Cys Ala Cys Cys Thr Thr Cys Gly Cys Cys Ala Thr Ala Cys Cys 1075 1080 1085 Thr Thr Thr Gly Gly Gly Thr Gly Cys Thr Ala Cys Cys Cys Ala Ala 1090 1095 1100 Gly Thr Ala Cys Cys Cys Thr Ala Thr Thr Ala Thr Thr Gly Thr Thr 1105 1110 1115 1120 Thr Thr Thr Thr Thr Ala Ala Ala Gly Thr Gly Gly Ala Thr Ala Cys 1125 1130 1135 Thr Thr Ala Cys Ala Ala Cys Thr Cys Cys Ala Cys Thr Gly Thr Thr 1140 1145 1150 Thr Ala Thr Ala Ala Ala Thr Thr Cys Thr Thr Gly Gly Cys Thr Gly 1155 1160 1165 Thr Thr Thr Thr Ala Cys Cys Thr Cys Cys Ala Ala Cys Cys Gly Thr 1170 1175 1180 Cys Ala Gly Gly Gly Ala Ala Ala Thr Thr Gly Thr Cys Ala Thr Cys 1185 1190 1195 1200 Ala Cys Cys Ala Ala Gly Thr Ala Thr Gly Gly Thr Gly Ala Thr Gly 1205 1210 1215 Thr Thr Thr Ala Thr Gly Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1220 1225 1230 Thr Gly Gly Ala Thr Ala Cys Thr Thr Gly Cys Ala Thr Cys Thr Cys 1235 1240 1245 Gly Gly Thr Thr Thr Gly Thr Thr Gly Gly Ala Thr Gly Cys Thr Gly 1250 1255 1260 Thr Cys Ala Cys Ala Ala Thr Thr Ala Ala Thr Thr Thr Cys Ala Cys 1265 1270 1275 1280 Thr Gly Gly Thr Cys Ala Thr Gly Gly Cys Ala Cys Thr Gly Ala Cys 1285 1290 1295 Gly Ala Thr Gly Ala Thr Gly Thr Thr Thr Cys Thr Gly Gly Thr Thr 1300 1305 1310 Thr Thr Thr Gly Gly Ala Cys Cys Ala Thr Ala Gly Cys Ala Thr Cys 1315 1320 1325 Gly Ala Cys Thr Ala Ala Thr Thr Thr Thr Gly Thr Thr Gly Ala Thr 1330 1335 1340 Gly Cys Ala Cys Thr Cys Ala Thr Cys Gly Ala Ala Gly Thr Thr Cys 1345 1350 1355 1360 Ala Ala Gly Gly Ala Ala Cys Cys Gly Cys Cys Ala Thr Thr Cys Ala 1365 1370 1375 Gly Cys Gly Thr Ala Thr Thr Cys Thr Thr Thr Ala Thr Thr Gly Thr 1380 1385 1390 Gly Ala Thr Gly Ala Thr Cys Cys Thr Gly Thr Thr Ala Gly Cys Cys 1395 1400 1405 Ala Ala Cys Thr Cys Ala Ala Gly Thr Gly Thr Thr Cys Thr Cys Ala 1410 1415 1420 Gly Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Ala Cys Cys Thr Thr 1425 1430 1435 1440 Gly Ala Cys Gly Ala Thr Gly Gly Thr Thr Thr Thr Thr Ala Cys Cys 1445 1450 1455 Cys Thr Ala Thr Thr Thr Cys Thr Thr Cys Thr Ala Gly Ala Ala Ala 1460 1465 1470 Cys Cys Thr Thr Cys Thr Gly Ala Gly Thr Cys Ala Thr Gly Ala Ala 1475 1480 1485 Cys Ala Gly Cys Cys Ala Ala Thr Thr Thr Cys Thr Thr Thr Thr Gly 1490 1495 1500 Thr Thr Ala Cys Thr Cys Thr Gly Cys Cys Ala Thr Cys Ala Thr Thr 1505 1510 1515 1520 Thr Ala Ala Thr Gly Ala Thr Cys Ala Thr Thr Cys Thr Thr Thr Thr 1525 1530 1535 Gly Thr Thr Ala Ala Cys Ala Thr Thr Ala Cys Thr Gly Thr Ala Thr 1540 1545 1550 Cys Thr Gly Cys Thr Thr Cys Cys Thr Thr Thr Gly Gly Thr Gly Gly 1555 1560 1565 Thr Cys Ala Thr Ala Gly Thr Gly Gly Thr Gly Cys Cys Ala Ala Cys 1570 1575 1580 Cys Thr Thr Ala Thr Thr Gly Cys Ala Thr Cys Thr Gly Ala Cys Ala 1585 1590 1595 1600 Cys Thr Ala Cys Thr Ala Thr Cys Ala Ala Thr Gly Gly Gly Thr Thr 1605 1610 1615 Thr Ala Gly Thr Thr Cys Thr Thr Thr Cys Thr Gly Thr Gly Thr Thr 1620 1625 1630 Gly Ala Cys Ala Cys Thr Ala Gly Ala Cys Ala Ala Thr Thr Thr Ala 1635 1640 1645 Cys Cys Ala Thr Thr Thr Cys Ala Cys Thr Gly Thr Thr Thr Thr Ala 1650 1655 1660 Thr Ala Ala Cys Gly Thr Thr Ala Cys Ala Ala Ala Cys Ala Gly Thr 1665 1670 1675 1680 Thr Ala Thr Gly Gly Thr Thr Ala Thr Gly Thr Gly Thr Cys Thr Ala 1685 1690 1695 Ala Ala Thr Cys Ala Cys Ala Gly Gly Ala Cys Ala Gly Thr Ala Ala 1700 1705 1710 Thr Thr Gly Cys Cys Cys Thr Thr Thr Cys Ala Cys Cys Thr Thr Gly 1715 1720 1725 Cys Ala Ala Thr Cys Thr Gly Thr Thr Ala Ala Thr Gly Ala Thr Thr 1730 1735 1740 Ala Cys Cys Thr Gly Thr Cys Thr Thr Thr Thr Ala Gly Cys Ala Ala 1745 1750 1755 1760 Ala Thr Thr Thr Thr Gly Thr Gly Thr Thr Thr Cys Cys Ala Cys Cys 1765 1770 1775 Ala Gly Cys Cys Thr Thr Thr Thr Gly Gly Cys Thr Ala Gly Thr Gly 1780 1785 1790 Cys Cys Thr Gly Thr Ala Cys Cys Ala Thr Ala Gly Ala Thr Cys Thr 1795 1800 1805 Thr Thr Thr Thr Gly Gly Thr Thr Ala Cys Cys Cys Thr Gly Ala Gly 1810 1815 1820 Thr Thr Thr Gly Gly Thr Ala Gly Thr Gly Gly Thr Gly Thr Thr Ala 1825 1830 1835 1840 Ala Gly Thr Thr Thr Ala Cys Gly Thr Cys Cys Cys Thr Thr Thr Ala 1845 1850 1855 Cys Thr Thr Thr Cys Ala Ala Thr Thr Cys Ala Cys Ala Ala Ala Gly 1860 1865 1870 Gly Gly Thr Gly Ala Gly Thr Thr Gly Ala Thr Thr Ala Cys Thr Gly 1875 1880 1885 Gly Cys Ala Cys Gly Cys Cys Thr Ala Ala Ala Cys Cys Ala Cys Thr 1890 1895 1900 Thr Gly Ala Ala Gly Gly Thr Gly Thr Cys Ala Cys Gly Gly Ala Cys 1905 1910 1915 1920 Gly Thr Thr Thr Cys Thr Thr Thr Thr Ala Thr Gly Ala Cys Thr Cys 1925 1930 1935 Thr Gly Gly Ala Thr Gly Thr Gly Thr Gly Thr Ala Cys Cys Ala Ala 1940 1945 1950 Gly Thr Ala Thr Ala Cys Thr Ala Thr Cys Thr Ala Thr Gly Gly Cys 1955 1960 1965 Thr Thr Thr Ala Ala Ala Gly Gly Thr Gly Ala Gly Gly Gly Thr Ala 1970 1975 1980 Thr Cys Cys Thr Thr Ala Cys Cys Cys Thr Thr Ala Cys Ala Ala Ala 1985 1990 1995 2000 Thr Thr Cys Thr Ala Gly Cys Thr Thr Thr Thr Thr Gly Gly Cys Ala 2005 2010 2015 Gly Gly Thr Gly Thr Thr Thr Ala Thr Thr Ala Cys Ala Cys Ala Thr 2020 2025 2030 Cys Thr Gly Ala Thr Thr Cys Thr Gly Gly Ala Cys Ala Gly Thr Thr 2035 2040 2045 Gly Thr Thr Ala Gly Cys Cys Thr Thr Thr Ala Ala Gly Ala Ala Thr 2050 2055 2060 Gly Thr Cys Ala Cys Thr Ala Gly Thr Gly Gly Thr Gly Cys Thr Gly 2065 2070 2075 2080 Thr Thr Thr Ala Thr Thr Cys Thr Gly Thr Thr Ala Cys Gly Cys Cys 2085 2090 2095 Ala Thr Gly Thr Thr Cys Thr Thr Thr Thr Thr Cys Ala Gly Ala Gly 2100 2105 2110 Cys Ala Gly Gly Cys Thr Gly Cys Ala Thr Ala Thr Gly Thr Thr Gly 2115 2120 2125 Ala Thr Gly Ala Thr Gly Ala Thr Ala Thr Ala Gly Thr Gly Gly Gly 2130 2135 2140 Thr Gly Thr Thr Ala Thr Thr Thr Cys Thr Ala Gly Thr Thr Thr Gly 2145 2150 2155 2160 Thr Cys Thr Ala Gly Cys Thr Cys Cys Ala Cys Thr Thr Thr Thr Ala 2165 2170 2175 Ala Cys Ala Gly Thr Ala Cys Thr Ala Gly Gly Gly Ala Gly Thr Thr 2180 2185 2190 Gly Cys Cys Thr Gly Gly Thr Thr Thr Cys Thr Thr Cys Thr Ala Cys 2195 2200 2205 Cys Ala Thr Thr Cys Thr Ala Ala Thr Gly Ala Thr Gly Gly Cys Thr 2210 2215 2220 Cys Thr Ala Ala Thr Thr Gly Thr Ala Cys Ala Gly Ala Gly Cys Cys 2225 2230 2235 2240 Thr Gly Thr Gly Thr Thr Gly Gly Thr Gly Thr Ala Thr Ala Gly Thr 2245 2250 2255 Ala Ala Cys Ala Thr Ala Gly Gly Thr Gly Thr Thr Thr Gly Thr Ala 2260 2265 2270 Ala Ala Thr Cys Thr Gly Gly Cys Ala Gly Thr Ala Thr Thr Gly Gly 2275 2280 2285 Cys Thr Ala Cys Gly Thr Cys Cys Cys Ala Thr Cys Thr Cys Ala Gly 2290 2295 2300 Thr Cys Thr Gly Gly Cys Cys Ala Ala Gly Thr Cys Ala Ala Gly Ala 2305 2310 2315 2320 Thr Thr 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Ala Ala Thr Gly Gly Cys Cys Thr Thr Gly Gly Thr Ala Cys Thr 2725 2730 2735 Gly Thr Thr Gly Ala Thr Gly Ala Ala Gly Ala Cys Thr Ala Thr Ala 2740 2745 2750 Ala Gly Cys Gly Cys Thr Gly Thr Thr Cys Thr Ala Ala Thr Gly Gly 2755 2760 2765 Thr Cys Gly Cys Thr Cys Thr Gly Thr Gly Gly Cys Ala Gly Ala Thr 2770 2775 2780 Cys Thr Ala Gly Thr Cys Thr Gly Thr Gly Cys Ala Cys Ala Gly Thr 2785 2790 2795 2800 Ala Thr Thr Ala Cys Thr Cys Thr Gly Gly Thr Gly Thr Cys Ala Thr 2805 2810 2815 Gly Gly Thr Ala Cys Thr Ala Cys Cys Thr Gly Gly Thr Gly Thr Thr 2820 2825 2830 Gly Thr Thr Gly Ala Cys Gly Cys Thr Gly Ala Gly Ala Ala Gly Cys 2835 2840 2845 Thr Thr Cys Ala Cys Ala Thr Gly Thr Ala Thr Ala Gly Thr Gly Cys 2850 2855 2860 Gly Thr Cys Thr Cys Thr Cys Ala Thr Cys Gly Gly Thr Gly Gly Thr 2865 2870 2875 2880 Ala Thr Gly Gly Thr Gly Cys Thr Ala Gly Gly Ala Gly Gly Thr Thr 2885 2890 2895 Thr Thr Ala Cys Thr Thr Cys Thr Gly Cys Ala Gly Cys Gly Gly Cys 2900 2905 2910 Ala Thr Thr Gly Cys Cys Thr Thr Thr Thr Ala Gly Cys Thr Ala Thr 2915 2920 2925 Gly Cys Thr Gly Thr Thr Cys Ala Ala Gly Cys Thr Ala Gly Ala Cys 2930 2935 2940 Thr Cys Ala Ala Thr Thr Ala Thr Cys Thr Thr Gly Cys Thr Cys Thr 2945 2950 2955 2960 Ala Cys Ala Gly Ala Cys Gly Gly Ala Thr Gly Thr Thr Cys Thr Ala 2965 2970 2975 Cys Ala Gly Cys Gly Gly Ala Ala Cys Cys Ala Gly Cys Ala Ala Thr 2980 2985 2990 Thr Gly Cys Thr Thr Gly Cys Thr Gly Ala Gly Thr Cys Thr Thr Thr 2995 3000 3005 Thr Ala Ala Cys Thr Cys Thr Gly Cys Thr Ala Thr Thr Gly Gly Thr 3010 3015 3020 Ala Ala Thr Ala Thr Ala Ala Cys Thr Thr Cys Ala Gly Cys Cys Thr 3025 3030 3035 3040 Thr Thr Gly Ala Gly Ala Gly Thr Gly Thr Thr Ala Ala Ala Gly Ala 3045 3050 3055 Gly Gly Cys Thr Ala Thr Thr Ala Gly Thr Cys Ala Ala Ala Cys Thr 3060 3065 3070 Thr Cys Cys Ala Ala Gly Gly Gly Thr Thr Thr Gly Ala Ala Cys Ala 3075 3080 3085 Cys Thr Gly Thr Gly Gly Cys Thr Cys Ala Thr Gly Cys Gly Cys Thr 3090 3095 3100 Thr Ala Cys Thr Ala Ala Gly Gly Thr Thr Cys Ala Ala Gly Ala Gly 3105 3110 3115 3120 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Thr Thr Cys Ala Gly Gly 3315 3320 3325 Cys Thr Ala Gly Cys Ala Gly Gly Ala Ala Gly Thr Thr Ala Gly Cys 3330 3335 3340 Ala Cys Ala Gly Cys Ala Ala Ala Ala Gly Gly Thr Thr Ala Ala Thr 3345 3350 3355 3360 Gly Ala Gly Thr Gly Cys Gly Thr Thr Ala Ala Ala Thr Cys Gly Cys 3365 3370 3375 Ala Ala Thr Cys Thr Cys Ala Gly Cys Gly Thr Thr Ala Thr Gly Gly 3380 3385 3390 Thr Thr Thr Thr Thr Gly Thr Gly Gly Thr Gly Gly Thr Gly Ala Thr 3395 3400 3405 Gly Gly Cys Gly Ala Gly Cys Ala Cys Ala Thr Thr Thr Thr Cys Thr 3410 3415 3420 Cys Thr Cys Thr Gly Gly Thr Ala Cys Ala Gly Gly Cys Ala Gly Cys 3425 3430 3435 3440 Ala Cys Cys Thr Cys Ala Gly Gly Gly Cys Cys Thr Gly Cys Thr Gly 3445 3450 3455 Thr Thr Thr Thr Thr Ala Cys Ala Thr Ala Cys Ala Gly Thr Ala Cys 3460 3465 3470 Thr Thr Gly Thr Ala Cys Cys Gly Ala Gly Thr Gly Ala Thr Thr Thr 3475 3480 3485 Thr Gly Thr Ala Gly Ala Thr Gly Thr Thr Ala Thr Thr Gly Cys Cys 3490 3495 3500 Ala Thr Cys Gly Cys Thr Gly Gly Cys Thr Thr Ala Thr Gly Cys Gly 3505 3510 3515 3520 Thr Thr Ala Ala Cys Gly Ala Thr Gly Ala Ala Ala Thr Thr Gly Cys 3525 3530 3535 Cys Thr Thr Gly Ala Cys Thr Cys Thr Ala Cys Gly Thr Gly Ala Gly 3540 3545 3550 Cys Cys Thr Gly Gly Cys Thr Thr Ala Gly Thr Cys Thr Thr Gly Thr 3555 3560 3565 Thr Thr Ala Cys Gly Cys Ala Thr Gly Ala Ala Cys Thr Thr Cys Ala 3570 3575 3580 Ala Ala Ala Thr Cys Ala Thr Ala Cys Thr Gly Cys Gly Ala Thr Gly 3585 3590 3595 3600 Gly Ala Ala Thr Ala Thr Thr Thr Thr Gly Thr Thr Thr Cys Ala Thr 3605 3610 3615 Cys Gly Cys Gly Ala Cys Gly Thr Ala Thr Gly Thr Thr Thr Gly Ala 3620 3625 3630 Ala Cys Cys Thr Ala Gly Ala Ala Ala Ala Cys Cys Thr Ala Cys Cys 3635 3640 3645 Gly Thr Thr Ala Gly Thr Gly Ala Thr Thr Thr Thr Gly Thr Thr Cys 3650 3655 3660 Ala Ala Ala Thr Thr Gly Ala Gly Ala Gly Thr Thr Gly Thr Gly Thr 3665 3670 3675 3680 Gly Gly Thr Cys Ala Cys Cys Thr Ala Thr Gly Thr Cys Ala Ala Thr 3685 3690 3695 Thr Thr Gly Ala Cys Thr Ala Gly Ala Gly Ala Cys Cys Ala Ala Cys 3700 3705 3710 Thr Ala Cys Cys Ala Gly Ala Thr Gly Thr Ala Ala Thr Cys Cys Cys 3715 3720 3725 Ala Gly Ala Thr Thr Ala Cys Ala Thr Cys Gly Ala Thr Gly Thr Thr 3730 3735 3740 Ala Ala Cys Ala Ala Ala Ala Cys Ala Cys Thr Thr Gly Ala Thr Gly 3745 3750 3755 3760 Ala Gly Ala Thr Thr Thr Thr Ala Gly Cys Thr Thr Cys Thr Cys Thr 3765 3770 3775 Gly Cys Cys Cys Ala Ala Thr Ala Gly Ala Ala Cys Thr Gly Gly Thr 3780 3785 3790 Cys Cys Ala Ala Gly Thr Cys Thr Thr Cys Cys Thr Thr Thr Ala Gly 3795 3800 3805 Ala Thr Gly Thr Thr Thr Thr Thr Ala Ala Thr Gly Cys Cys Ala Cys 3810 3815 3820 Thr Thr Ala Thr Cys Thr Thr Ala Ala Thr Cys Thr Cys Ala Cys Thr 3825 3830 3835 3840 Gly Gly Thr Gly Ala Ala Ala Thr Thr Gly Cys Ala Gly Ala Thr Thr 3845 3850 3855 Thr Ala Cys Ala Gly Cys Ala Gly Cys Gly Thr Thr Cys Ala Gly Ala 3860 3865 3870 Gly Thr Cys Thr Cys Thr Cys Cys Gly Thr Ala Ala Thr Ala Cys Thr 3875 3880 3885 Ala Cys Ala Gly Ala Gly Gly Ala Gly Cys Thr Cys Cys Ala Ala Ala 3890 3895 3900 Gly Thr Cys Thr Thr Ala Thr Ala Thr Ala Thr Ala Ala Thr Ala Thr 3905 3910 3915 3920 Cys Ala Ala Cys Ala Ala Cys Ala Cys Ala Cys Thr Ala Gly Thr Thr 3925 3930 3935 Gly Ala Cys Cys Thr Thr Gly Ala Gly Thr Gly Gly Cys Thr Cys Ala 3940 3945 3950 Ala Cys Cys Gly Ala Gly Thr Thr Gly Ala Gly Ala Cys Ala Thr Ala 3955 3960 3965 Thr Ala Thr Cys Ala Ala Gly Thr Gly Gly Cys Cys Gly Thr Gly Gly 3970 3975 3980 Thr Gly Gly Gly Thr Thr Thr Gly Gly Thr Thr Gly Ala Thr Thr Ala 3985 3990 3995 4000 Thr Thr Thr Thr Cys Ala Thr Thr Gly Thr Thr Cys Thr Cys Ala Thr 4005 4010 4015 Cys Thr Thr Thr Gly Thr Thr Gly Thr Gly Thr Cys Ala Thr Thr Ala 4020 4025 4030 Cys Thr Ala Gly Thr Gly Thr Thr Cys Thr Gly Cys Thr Gly Cys Ala 4035 4040 4045 Thr Thr Thr Cys Cys Ala Cys Gly Gly Gly Thr Thr Gly Thr Thr Gly 4050 4055 4060 Thr Gly Gly Ala Thr Gly Cys Thr Gly Cys Gly Gly Cys Thr Gly Cys 4065 4070 4075 4080 Thr Gly Cys Thr Gly Thr Gly Cys Thr Thr Gly Thr Thr Thr Cys Thr 4085 4090 4095 Cys Ala Gly Gly Thr Thr Gly Thr Thr Gly Thr Ala Gly Gly Gly Gly 4100 4105 4110 Thr Cys Cys Thr Ala Gly Ala Cys Thr Thr Cys Ala Ala Cys Cys Thr 4115 4120 4125 Thr Ala Cys Gly Ala Ala Gly Thr Thr Thr Thr Thr Gly Ala Ala Ala 4130 4135 4140 Ala Gly Gly Thr Cys Cys Ala Cys Gly Thr Gly Cys Ala Gly Thr Gly 4145 4150 4155 4160 Ala Thr Gly Thr Thr Thr Cys Thr Thr Gly Gly Ala Cys Thr Thr Thr 4165 4170 4175 Thr Thr Cys Ala Ala Thr Ala Cys Ala Cys Gly Ala Thr Thr Gly Ala 4180 4185 4190 Cys Ala Cys Ala Gly Thr Thr Gly Thr Cys Ala Ala Ala Gly Ala Thr 4195 4200 4205 Gly Thr Cys Thr Cys Ala Ala Ala Gly Thr Cys Thr Gly Cys Thr Ala 4210 4215 4220 Ala Cys Thr Thr Gly Thr Cys Thr Thr Thr Gly Thr Ala Thr Gly Cys 4225 4230 4235 4240 Thr Gly Thr Cys Cys Ala Ala Gly Ala Gly Thr Thr Gly Gly Ala Gly 4245 4250 4255 Cys Thr Cys Ala Ala Thr Gly Thr Ala Gly Thr Thr Cys Cys Ala Ala 4260 4265 4270 Thr Thr Ala Gly Ala Cys Ala Ala Gly Cys Thr Thr Cys Ala Ala Ala 4275 4280 4285 Thr Gly Thr Gly Ala Cys Gly Gly Gly Thr Thr Thr Thr Cys Thr Thr 4290 4295 4300 Thr Thr Cys Ala Cys Cys Ala Gly Thr Gly Thr Thr Thr Thr Thr Ala 4305 4310 4315 4320 Thr Cys Thr Ala Cys Thr Thr Cys Thr Thr Thr Gly Cys Ala Cys Thr 4325 4330 4335 Gly Thr Thr Thr Ala Ala Ala Gly Cys Gly Thr Cys Thr Thr Cys Thr 4340 4345 4350 Thr Thr Gly Ala Gly Gly Cys Gly Cys Ala Ala Thr Thr Ala Thr Ala 4355 4360 4365 Thr Thr Ala Thr Gly Thr Thr Gly Gly Cys Ala Gly Cys Gly Cys Gly 4370 4375 4380 Thr Thr Thr Thr Gly Cys Thr Gly Thr Cys Ala Thr Thr Gly Thr Thr 4385 4390 4395 4400 Cys Thr Thr Thr Ala Thr Thr Gly Cys Cys Cys Ala Cys Thr Thr Thr 4405 4410 4415 Thr Ala Thr Ala Thr Thr Ala Thr Thr Gly Thr Gly Gly Thr Gly Cys 4420 4425 4430 Ala Thr Thr Thr Thr Thr Ala Gly Ala Thr Gly Cys Ala Ala Cys Thr 4435 4440 4445 Ala Thr Thr Ala Thr Thr Thr Gly Thr Thr Gly Cys Ala Cys Ala Cys 4450 4455 4460 Thr Thr Ala Thr Thr Gly Gly Cys Ala Gly Gly Cys Thr Thr Thr Gly 4465 4470 4475 4480 Thr Thr Thr Ala Gly Thr Cys Thr Gly Cys Thr Thr Thr Thr Ala Cys 4485 4490 4495 Thr Cys Cys Thr Gly Gly Cys Gly Cys Thr Ala Thr Ala Ala Ala Ala 4500 4505 4510 Ala Thr Gly Cys Gly Cys Thr Cys Thr Thr Thr Ala Thr Thr Ala Thr 4515 4520 4525 Thr Thr Thr Thr Ala Ala Thr Ala Cys Thr Ala Cys Gly Ala Cys Ala 4530 4535 4540 Cys Thr Thr Thr Cys Thr Thr Thr Cys Cys Thr Cys Ala Ala Thr Gly 4545 4550 4555 4560 Gly Thr Ala Ala Ala Gly Cys Ala Gly Cys Thr Thr Ala Thr Thr Ala 4565 4570 4575 Thr Gly Ala Cys Gly Gly Cys Ala Ala Ala Thr Cys Cys Ala Thr Thr 4580 4585 4590 Gly Thr Gly Ala Thr Thr Thr Thr Ala Gly Ala Ala Gly Gly Thr Gly 4595 4600 4605 Gly Thr Gly Ala Cys Cys Ala Thr Thr Ala Cys Ala Thr Cys Ala Cys 4610 4615 4620 Thr Thr Thr Thr Gly Gly Cys Ala Ala Cys Thr Cys Thr Thr Thr Thr 4625 4630 4635 4640 Gly Thr Thr Gly Cys Thr Thr Thr Thr Gly Thr Thr Ala Gly Thr Ala 4645 4650 4655 Gly Cys Ala Thr Cys Gly Ala Cys Thr Thr Gly Thr Ala Thr Cys Thr 4660 4665 4670 Ala Gly Cys Thr Ala Thr Ala Cys Gly Thr Gly Gly Gly Cys Gly Gly 4675 4680 4685 Cys Ala Ala Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Thr Ala Cys 4690 4695 4700 Ala Gly Cys Thr Gly Thr Thr Gly Cys Gly Ala Ala Cys Thr Gly Thr 4705 4710 4715 4720 Thr Gly Ala Gly Cys Thr Thr Cys Thr Thr Gly Ala Thr Gly Gly Cys 4725 4730 4735 Ala Ala Gly Ala Ala Gly Cys Thr Thr Thr Ala Thr Gly Thr Cys Thr 4740 4745 4750 Thr Thr Thr Cys Gly Cys Ala Ala Cys Ala Thr Cys Ala Ala Ala Thr 4755 4760 4765 Thr Gly Thr Thr Gly Gly Cys Ala Thr Thr Ala Cys Thr Ala Ala Thr 4770 4775 4780 Gly Cys Thr Gly Cys Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 4785 4790 4795 4800 Thr Thr Cys Ala Ala Cys Thr Ala Gly Ala Cys Gly Ala Gly Thr Ala 4805 4810 4815 Thr Gly Cys Thr Ala Cys Ala Ala Thr Thr Ala Gly Thr Gly Ala Ala 4820 4825 4830 Thr Gly Ala Thr Ala Ala Thr Gly Gly Thr Cys Thr Ala Gly Thr Ala 4835 4840 4845 Gly Thr Thr Ala Ala Thr Gly Thr Thr Ala Thr Ala Cys Thr Thr Thr 4850 4855 4860 Gly Gly Cys Thr Thr Thr Thr Cys Gly Thr Ala Cys Thr Cys Thr Thr 4865 4870 4875 4880 Thr Thr Thr Cys Cys Thr Gly Cys Thr Thr Ala Thr Thr Ala Thr Ala 4885 4890 4895 Ala Gly Cys Ala Thr Thr Ala Cys Thr Thr Thr Cys Gly Thr Cys Cys 4900 4905 4910 Ala Ala Thr Thr Gly Gly Thr Thr Ala Ala Thr Cys Thr Gly Thr Gly 4915 4920 4925 Cys Thr Thr Cys Ala Cys Thr Thr Gly Thr Cys Ala Cys Cys Gly Gly 4930 4935 4940 Thr Thr Gly Thr Gly Thr Ala Ala Thr Ala Gly Cys Gly Cys Ala Gly 4945 4950 4955 4960 Thr Thr Thr Ala Cys Ala Cys Ala Cys Cys Thr Ala Thr Ala Gly Gly 4965 4970 4975 Gly Cys Gly Thr Thr Thr Gly Thr Ala Thr Ala Gly Ala Gly Thr Thr 4980 4985 4990 Thr Ala Thr Ala Ala Gly Thr Cys Thr Thr Ala Cys Ala Thr Gly Cys 4995 5000 5005 Ala Ala Ala Thr Ala Gly Ala Cys Cys Cys Cys Cys Thr Cys Thr Cys 5010 5015 5020 Thr Ala Gly Thr Ala Cys Thr Gly Thr Thr Ala Thr Thr Gly Ala Cys 5025 5030 5035 5040 Gly Thr Ala Thr Ala Ala Ala Cys Gly Ala Ala Ala Thr Ala Thr Gly 5045 5050 5055 Thr Cys Thr Ala Ala Cys Gly Gly Thr Thr Cys Thr Ala Thr Thr Cys 5060 5065 5070 Cys Cys Gly Thr Thr Gly Ala Thr Gly Ala Gly Gly Thr Gly Ala Thr 5075 5080 5085 Thr Cys Ala Ala Cys Ala Cys Cys Thr Thr Ala Gly Ala Ala Ala Cys 5090 5095 5100 Thr Gly Gly Ala Ala Thr Thr Thr Cys Ala Cys Ala Thr Gly Gly Ala 5105 5110 5115 5120 Ala Thr Ala Thr Cys Ala Thr Ala Cys Thr Gly Ala Cys Gly Ala Thr 5125 5130 5135 Ala Cys Thr Ala Cys Thr Thr Gly Thr Ala Gly Thr Gly Cys Thr Thr 5140 5145 5150 Cys Ala Gly Thr Ala Thr Gly Gly Cys Cys Ala Thr Thr Ala Cys Ala 5155 5160 5165 Ala Gly Thr Ala Cys Thr Cys Thr Gly Cys Gly Thr Thr Cys Thr Thr 5170 5175 5180 Gly Thr Ala Thr Gly Gly Thr Gly Thr Cys Ala Ala Gly Ala Thr Gly 5185 5190 5195 5200 Gly Cys Thr Ala Thr Thr Cys Thr Ala Thr Gly Gly Ala Thr Ala Cys 5205 5210 5215 Thr Thr Thr Gly Gly Cys Cys Thr Cys Thr Thr Gly Thr Gly Thr Thr 5220 5225 5230 Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Cys Thr Thr Thr Thr Thr 5235 5240 5245 Gly Ala Thr Gly Cys Ala Thr Gly Gly Gly Cys Thr Ala Gly Cys Thr 5250 5255 5260 Thr Thr Cys Ala Gly Gly Thr Cys Ala Ala Thr Thr Gly Gly Gly Thr 5265 5270 5275 5280 Cys Thr Thr Thr Thr Thr Thr Gly Cys Thr Thr Thr Cys Ala Gly Cys 5285 5290 5295 Ala Thr Cys Cys Thr Thr Ala Thr Gly Gly Cys Thr Thr Gly Cys Ala 5300 5305 5310 Thr Cys Ala Cys Thr Cys Thr Thr Ala Thr Gly Cys Thr Gly Thr Gly 5315 5320 5325 Gly Ala Thr Ala Ala Thr Gly Thr Ala Cys Thr Thr Thr Gly Thr Cys 5330 5335 5340 Ala Ala Thr Ala Gly Cys Ala Thr Thr Cys Gly Gly Thr Thr Gly Thr 5345 5350 5355 5360 Gly Gly Cys Gly Cys Ala Gly Gly Ala Cys Ala Cys Ala Thr Thr Cys 5365 5370 5375 Thr Thr Gly Gly Thr Gly Gly Thr Cys Thr Thr Thr Cys Ala Ala Thr 5380 5385 5390 Cys Cys Thr Gly Ala Ala Ala Cys Ala Gly Ala Cys Gly Cys Gly Cys 5395 5400 5405 Thr Thr Cys Thr Cys Ala Cys Thr Ala Cys Thr Thr Cys Thr Gly Thr 5410 5415 5420 Gly Ala Thr Gly Gly Gly Cys Cys Gly Ala Cys Ala Gly Gly Thr Cys 5425 5430 5435 5440 Thr Gly Cys Ala Thr Thr Cys Cys Ala Gly Thr Gly Cys Thr Thr Gly 5445 5450 5455 Gly Ala Gly Cys Ala Cys Cys Ala Ala Cys Thr Gly Gly Thr Gly Thr 5460 5465 5470 Ala Ala Cys Gly Cys Thr Ala Ala Cys Ala Cys Thr Cys Cys Thr Thr 5475 5480 5485 Ala Gly Thr Gly Gly Thr Ala Cys Ala Thr Thr Gly Cys Thr Thr Gly 5490 5495 5500 Thr Ala Gly Ala Gly Gly Gly Cys Thr Ala Thr Ala Ala Gly Gly Thr 5505 5510 5515 5520 Thr Gly Cys Thr Ala Cys Thr Gly Gly Cys Gly Thr Ala Cys Ala Gly 5525 5530 5535 Gly Thr Ala Ala Gly Thr Cys Ala Ala Thr Thr Ala Cys Cys Thr Ala 5540 5545 5550 Ala Thr Thr Thr Cys Gly Thr Cys Ala Cys Ala Gly Thr Cys Gly Cys 5555 5560 5565 Cys Ala Ala Gly Gly Cys Cys Ala Cys Thr Ala Cys Ala Ala Cys Ala 5570 5575 5580 Ala Thr Thr Gly Thr Cys Thr Ala Cys Gly Gly Ala Cys Gly Thr Gly 5585 5590 5595 5600 Thr Thr Gly Gly Thr Cys Gly Thr Thr Cys Ala Gly Thr Cys Ala Ala 5605 5610 5615 Thr Gly Cys Thr Thr Cys Ala Thr Cys Thr Gly Gly Cys Ala Cys Thr 5620 5625 5630 Gly Gly Thr Thr Gly Gly Gly Cys Thr Thr Thr Cys Thr Ala Thr Gly 5635 5640 5645 Thr Cys Cys Gly Gly Thr Cys Cys Ala Ala Ala Cys Ala Cys Gly Gly 5650 5655 5660 Cys Gly Ala Cys Thr Ala Cys Thr Cys Ala Gly Cys Thr Gly Thr Gly 5665 5670 5675 5680 Ala Gly Thr Ala Ala Thr Cys Cys Gly Ala Gly Thr Thr Cys Gly Gly 5685 5690 5695 Thr Thr Cys Thr Cys Ala Cys Ala Gly Ala Thr Ala Gly Thr Gly Ala 5700 5705 5710 Gly Ala Ala Ala Gly Thr Gly Cys Thr Thr Cys Ala Thr Thr Thr Ala 5715 5720 5725 Gly Thr Cys Thr Ala Ala Ala Cys Ala Gly Ala Ala Ala Cys Thr Thr 5730 5735 5740 Thr Ala Thr Gly Gly Cys Thr Thr Cys Thr Gly Thr Cys Ala Gly Thr 5745 5750 5755 5760 Thr Thr Thr Cys Ala Gly Gly Ala Thr Cys Gly Thr Gly Gly Cys Cys 5765 5770 5775 Gly Cys Ala Ala Ala Cys Gly Gly Gly Thr Gly Cys Cys Ala Thr Thr 5780 5785 5790 Ala Thr Cys Cys Cys Thr Cys Thr Ala Thr Gly Cys Cys Cys Cys Thr 5795 5800 5805 Cys Thr Thr Ala Gly Gly Gly Thr Thr Ala Cys Thr Ala Ala Thr Gly 5810 5815 5820 Ala Cys Ala Ala Ala Cys Cys Cys Cys Thr Thr Thr Cys Thr Ala Ala 5825 5830 5835 5840 Gly Gly Thr Ala Cys Thr Thr Gly Cys Ala Ala Ala Thr Ala Ala Thr 5845 5850 5855 Gly Cys Thr Gly Thr Ala Cys Cys Cys Ala Cys Thr Ala Ala Thr Ala 5860 5865 5870 Ala Ala Gly Gly Ala Ala Ala Thr Ala Ala Gly Gly Ala Cys Cys Ala 5875 5880 5885 Gly Cys Ala Ala Ala Thr Thr Gly Gly Ala Thr Ala Cys Thr Gly Gly 5890 5895 5900 Ala Ala Thr Gly Ala Gly Cys Ala Ala Ala Thr Thr Cys Gly Cys Thr 5905 5910 5915 5920 Gly Gly Cys Gly Cys Ala Thr Gly Cys Gly Cys Cys Gly Thr Gly Gly 5925 5930 5935 Thr Gly Ala Gly Cys Gly Ala Ala Thr Thr Gly Ala Ala Cys Ala Ala 5940 5945 5950 Cys Cys Thr Thr Cys Cys Ala Ala Thr Thr Gly Gly Cys Ala Thr Thr 5955 5960 5965 Thr Cys Thr Ala Cys Thr Ala Cys Cys Thr Cys Gly Gly Ala Ala Cys 5970 5975 5980 Ala Gly Gly Ala Cys Cys Thr Cys Ala Cys Gly Cys Cys Gly Ala Cys 5985 5990 5995 6000 Cys Thr Cys Cys Gly Cys Thr Ala Thr Ala Gly Gly Ala Cys Thr Cys 6005 6010 6015 Gly Thr Ala Cys Thr Gly Ala Gly Gly Gly Thr Gly Thr Thr Thr Thr 6020 6025 6030 Cys Thr Gly Gly Gly Thr Thr Gly Cys Thr Ala Ala Ala Gly Ala Ala 6035 6040 6045 Gly Gly Cys Gly Cys Ala Ala Ala Gly Ala Cys Thr Gly Ala Ala Cys 6050 6055 6060 Cys Cys Ala Cys Thr Ala Ala Cys Cys Thr Gly Gly Gly Thr Gly Thr 6065 6070 6075 6080 Cys Ala Gly Ala Ala Ala Gly Gly Cys Gly Thr Cys Thr Gly Ala Ala 6085 6090 6095 Ala Ala Gly Cys Cys Ala Ala Thr Thr Ala Thr Thr Cys Cys Ala Ala 6100 6105 6110 Ala Thr Thr Thr Cys Thr Cys Thr Cys Ala Ala Cys Ala Gly Cys Thr 6115 6120 6125 Thr Cys Cys Cys Ala Gly Cys Gly Thr Ala Gly Thr Thr Gly Ala Gly 6130 6135 6140 Ala Thr Thr Gly Thr Thr Gly Ala Ala Cys Cys Thr Ala Ala Cys Ala 6145 6150 6155 6160 Cys Ala Cys Cys Thr Cys Cys Thr Ala Cys Thr Thr Cys Ala Cys Gly 6165 6170 6175 Thr Gly Cys Ala Ala Ala Thr Thr Cys Ala Cys Gly Thr Ala Gly Cys 6180 6185 6190 Ala Gly Gly Ala Gly Thr Cys Gly Thr Gly Gly Thr Ala Ala Thr Gly 6195 6200 6205 Gly Cys Ala Ala Cys Ala Ala Cys Ala Gly Gly Thr Cys Cys Ala Gly 6210 6215 6220 Ala Thr Cys Thr Cys Cys Ala Ala Gly Thr Ala Ala Cys Ala Ala Cys 6225 6230 6235 6240 Ala Gly Ala Gly Gly Cys Ala Ala Thr Ala Ala Cys Cys Ala Gly Thr 6245 6250 6255 Cys Cys Cys Gly Cys Gly Gly Thr Ala Ala Thr Thr Cys Ala Cys Ala 6260 6265 6270 Gly Ala Ala Thr Cys Gly Thr Gly Gly Ala Ala Ala Thr Ala Ala Cys 6275 6280 6285 Cys Ala Gly Gly Gly Thr Cys Gly Thr Gly Gly Ala Gly Cys Thr Thr 6290 6295 6300 Cys Thr Cys Ala Gly Ala Ala Cys Ala Gly Ala Gly Gly Ala Gly Gly 6305 6310 6315 6320 Cys Ala Ala Thr Ala Ala Thr Ala Ala Thr Ala Ala Cys Ala Ala Thr 6325 6330 6335 Ala Ala Cys Ala Ala Gly Thr Cys Thr Cys Gly Thr Ala Ala Cys Cys 6340 6345 6350 Ala Gly Thr Cys Cys Ala Ala Gly Ala Ala Cys Ala Gly Ala Ala Ala 6355 6360 6365 Cys Cys Ala Gly Thr Cys Ala Ala Ala Thr Gly Ala Cys Cys Gly Thr 6370 6375 6380 Gly Gly Thr Gly Gly Thr Gly Thr Ala Ala Cys Ala Thr Cys Ala Cys 6385 6390 6395 6400 Gly Cys Gly Ala Thr Gly Ala Thr Cys Thr Gly Gly Thr Gly Gly Cys 6405 6410 6415 Thr Gly Cys Thr Gly Thr Cys Ala Ala Gly Gly Ala Thr Gly Cys Cys 6420 6425 6430 Cys Thr Thr Ala Ala Ala Thr Cys Thr Thr Thr Gly Gly Gly Thr Ala 6435 6440 6445 Thr Thr Gly Gly Cys Gly Ala Ala Ala Ala Cys Cys Cys Thr Gly Ala 6450 6455 6460 Cys Ala Ala Gly Cys Thr Thr Ala Ala Gly Cys Ala Ala Cys Ala Gly 6465 6470 6475 6480 Cys Ala Gly Ala Ala Gly Cys Cys Cys Ala Ala Ala Cys Ala Gly Gly 6485 6490 6495 Ala Ala Ala Gly Gly Thr Cys Thr Gly Ala Cys Ala Gly Cys Ala Gly 6500 6505 6510 Cys Gly Gly Cys Ala Ala Ala Ala Ala Thr Ala Cys Ala Cys Cys Thr 6515 6520 6525 Ala Ala Gly Ala Ala Gly Ala Ala Cys Ala Ala Ala Thr Cys Cys Ala 6530 6535 6540 Gly Ala Gly Cys Cys Ala Cys Thr Thr Cys Gly Ala Ala Ala Gly Ala 6545 6550 6555 6560 Ala Cys Gly Thr Gly Ala Cys Cys Thr Cys Ala Ala Ala Gly Ala Cys 6565 6570 6575 Ala Thr Cys Cys Cys Ala Gly Ala Gly Thr Gly Gly Ala Gly Gly Ala 6580 6585 6590 Gly Ala Ala Thr Thr Cys Cys Cys Ala Ala Gly Gly Gly Cys Gly Ala 6595 6600 6605 Ala Ala Ala Thr Ala Gly Cys Gly Thr Ala Gly Cys Ala Gly Cys Thr 6610 6615 6620 Thr Gly Cys Thr Thr Cys Gly Gly Ala Cys Cys Cys Ala Gly Gly Gly 6625 6630 6635 6640 Gly Ala Gly Gly Cys Thr Thr Cys Ala Ala Ala Ala Ala Thr Thr Thr 6645 6650 6655 Thr Gly Gly Ala Gly Ala Thr Gly Cys Gly Gly Ala Ala Thr Thr Thr 6660 6665 6670 Gly Thr Cys Gly Ala Ala Ala Ala Ala Gly Gly Thr Gly Thr Thr Gly 6675 6680 6685 Ala Thr Gly Cys Cys Thr Cys Ala Gly Gly Cys Thr Ala Thr Gly Cys 6690 6695 6700 Thr Cys Ala Gly Ala Thr Cys Gly Cys Cys Ala Gly Thr Thr Thr Ala 6705 6710 6715 6720 Gly Cys Ala Cys Cys Ala Ala Ala Thr Gly Thr Thr Gly Cys Ala Gly 6725 6730 6735 Cys Ala Thr Thr Gly Cys Thr Cys Thr Thr Thr Gly Gly Thr Gly Gly 6740 6745 6750 Thr Ala Ala Thr Gly Thr Gly Gly Cys Thr Gly Thr Thr Cys Gly Thr 6755 6760 6765 Gly Ala Gly Cys Thr Ala Gly Cys Gly Gly Ala Cys Thr Cys Thr Thr 6770 6775 6780 Ala Cys Gly Ala Gly Ala Thr Thr Ala Cys Ala Thr Ala Thr Ala Ala 6785 6790 6795 6800 Thr Thr Ala Thr Ala Ala Ala Ala Thr Gly Ala Cys Thr Gly Thr Gly 6805 6810 6815 Cys Cys Ala Ala Ala Gly Thr Cys Thr Gly Ala Thr Cys Cys Ala Ala 6820 6825 6830 Ala Thr Gly Thr Ala Gly Ala Gly Cys Thr Thr Cys Thr Thr Gly Thr 6835 6840 6845 Thr Thr Cys Ala Cys Ala Gly Gly Thr Gly Gly Ala Thr Gly Cys Ala 6850 6855 6860 Thr Thr Thr Ala Ala Ala Ala Cys Thr Gly Gly Gly Ala Ala Thr Gly 6865 6870 6875 6880 Cys Ala Ala Ala Ala Cys Cys Cys Cys Ala Gly Ala Gly Ala Ala Ala 6885 6890 6895 Gly Ala Ala Gly Gly Ala Ala Ala Ala Gly Ala Ala Gly Ala Ala Cys 6900 6905 6910 Ala Ala Gly Cys Gly Thr Gly Ala Ala Ala Cys Cys Ala Cys Gly Cys 6915 6920 6925 Ala Gly Cys Ala Gly Cys Thr Gly Ala Ala Thr Gly Ala Ala Gly Ala 6930 6935 6940 Gly Gly Cys Cys Ala Thr Cys Thr Ala Cys Gly Ala Thr Gly Ala Thr 6945 6950 6955 6960 Gly Thr Gly Gly Gly Thr Gly Thr Gly Cys Cys Ala Thr Cys Thr Gly 6965 6970 6975 Ala Thr Gly Thr Gly Ala Cys Thr Cys Ala Thr Gly Cys Cys Ala Ala 6980 6985 6990 Thr Thr Thr Gly Gly Ala Ala Thr Gly Gly Gly Ala Cys Ala Cys Ala 6995 7000 7005 Gly Cys Thr Gly Thr Thr Gly Ala Thr Gly Gly Thr Gly Gly Thr Gly 7010 7015 7020 Ala Cys Ala Cys Gly Gly Cys Cys Gly Thr Thr Gly Ala Ala Ala Thr 7025 7030 7035 7040 Thr Ala Thr Cys Ala Ala Cys Gly Ala Gly Ala Thr Cys Thr Thr Cys 7045 7050 7055 Gly Ala Cys Ala Cys Ala Gly Gly Ala Ala Ala Thr Thr Ala Ala Ala 7060 7065 7070 Cys Ala Ala Thr Gly Thr Thr Thr Gly Ala Cys Thr Gly Gly Cys Thr 7075 7080 7085 Thr Ala Thr Cys Cys Thr Gly Gly Cys Thr Ala Thr Gly Thr Cys Cys 7090 7095 7100 Cys Ala Gly Gly Gly Thr Ala Gly Thr Gly Cys Cys Ala Thr Thr Ala 7105 7110 7115 7120 Cys Ala Cys Thr Gly Thr Thr Ala Thr Thr Ala Cys Thr Gly Ala Gly 7125 7130 7135 Thr Gly Thr Thr Thr Thr Thr Cys Thr Ala Gly Cys Gly Ala Cys Thr 7140 7145 7150 Thr Gly Gly Cys Thr Gly Cys Thr Gly Gly Gly Cys Thr Ala Thr Gly 7155 7160 7165 Gly Cys Thr Thr Thr Gly Cys Cys Cys Thr Cys Thr Ala Ala Cys Thr 7170 7175 7180 Ala Gly Cys Gly Gly Thr Cys Thr Thr Gly Gly Thr Cys Thr Thr Gly 7185 7190 7195 7200 Cys Ala Cys Ala Cys Ala Ala Cys Gly Gly Thr Ala Ala Gly Cys Cys 7205 7210 7215 Ala Gly Thr Gly Gly Thr Ala Ala Thr Gly Thr Cys Ala Gly Thr Gly 7220 7225 7230 Cys Ala Ala Gly Ala Ala Gly Gly Ala Thr Ala Thr Thr Ala Cys Cys 7235 7240 7245 Ala Thr Ala Gly Cys Ala Cys Thr Gly Thr Cys Ala Thr Gly Ala Gly 7250 7255 7260 Gly Gly Gly Ala Ala Cys Gly Cys Ala Gly Thr Ala Cys Cys Thr Thr 7265 7270 7275 7280 Thr Thr Cys Ala Thr Cys Thr Ala Ala Ala Cys Cys Thr Thr Thr Gly 7285 7290 7295 Cys Ala Cys Gly Ala Gly Thr Ala Ala Thr Cys Ala Ala Ala Gly Ala 7300 7305 7310 Thr Cys Cys Gly Cys Thr Thr Gly Ala Cys Gly Ala Gly Cys Cys Thr 7315 7320 7325 Ala Thr Ala Thr Gly Gly Ala Ala Gly Ala Gly Cys Gly Thr Gly Cys 7330 7335 7340 Cys Ala Gly Gly Thr Ala Thr Thr Thr Gly Ala Cys Thr Cys Ala Ala 7345 7350 7355 7360 Gly Gly Ala Cys Thr Gly Thr Thr Ala Gly Thr Ala Ala Cys Thr Gly 7365 7370 7375 Ala Ala Gly Ala Cys Cys Thr Gly Ala Cys Gly Gly Thr Gly Thr Thr 7380 7385 7390 Gly Ala Thr Ala Thr Gly Gly Ala Thr Ala Cys Ala Cys 7395 7400 7405

Claims (23)

  1. 서열번호 1의 아미노산 서열 또는 이와 98% 이상의 상동성을 가지는 아미노산 서열로 이루어지며 동등한 면역원성을 가지는 스파이크 단백질을 포함하는 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  2. 청구항 1에 있어서, 상기 98% 이상의 상동성을 가지는 아미노산 서열은 서열번호 8, 15, 22, 28, 34, 및 40으로 이루어진 군으로부터 선택된 아미노산 서열인 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  3. 청구항 1에 있어서, 상기 분리주는 서열번호 2의 ORF (open reading frame) 1a/1b, 서열번호 3의 ORF 3, 서열번호 4의 외피 단백질 (envelope protein), 서열번호 5의 막단백질 (membrane protein), 및 서열번호 6의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열 또는 이와 98% 이상의 상동성을 가지는 아미노산 서열을 더 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  4. 청구항 3에 있어서, 상기 분리주는 서열번호 7의 아미노산 서열로 이루어진 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  5. 청구항 3에 있어서, 상기 분리주는 수탁번호 KCTC12736BP로 수탁된 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  6. 청구항 1에 있어서, 상기 분리주는 서열번호 8의 스파이크 단백질, 서열번호 9의 ORF (open reading frame) 1a/1b, 서열번호 10의 ORF 3, 서열번호 11의 외피 단백질 (envelope protein), 서열번호 12의 막단백질 (membrane protein), 및 서열번호 13의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  7. 청구항 6에 있어서, 상기 분리주는 서열번호 14의 아미노산 서열로 이루어진 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  8. 청구항 1에 있어서, 상기 분리주는 서열번호 15의 스파이크 단백질, 서열번호 16의 ORF (open reading frame) 1a/1b, 서열번호 17의 ORF 3, 서열번호 18의 외피 단백질 (envelope protein), 서열번호 19의 막단백질 (membrane protein), 및 서열번호 20의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  9. 청구항 8에 있어서, 상기 분리주는 서열번호 21의 아미노산 서열로 이루어진 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  10. 청구항 1에 있어서, 상기 분리주는 서열번호 22의 스파이크 단백질, 서열번호 23의 ORF 3, 서열번호 24의 외피 단백질 (envelope protein), 서열번호 25의 막단백질 (membrane protein), 및 서열번호 26의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  11. 청구항 10에 있어서, 상기 분리주는 서열번호 27의 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  12. 청구항 1에 있어서, 상기 분리주는 서열번호 28의 스파이크 단백질, 서열번호 29의 ORF 3, 서열번호 30의 외피 단백질 (envelope protein), 서열번호 31의 막단백질 (membrane protein), 및 서열번호 32의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  13. 청구항 12에 있어서, 상기 분리주는 서열번호 33의 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  14. 청구항 1에 있어서, 상기 분리주는 서열번호 34의 스파이크 단백질, 서열번호 35의 ORF 3, 서열번호 36의 외피 단백질 (envelope protein), 서열번호 37의 막단백질 (membrane protein), 및 서열번호 38의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  15. 청구항 14에 있어서, 상기 분리주는 서열번호 39의 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  16. 청구항 1에 있어서, 상기 분리주는 서열번호 40의 스파이크 단백질, 서열번호 41의 ORF 3, 서열번호 42의 외피 단백질 (envelope protein), 서열번호 43의 막단백질 (membrane protein), 및 서열번호 44의 뉴로캡사이드 단백질 (nucleocapsid protein)으로 이루어진 군으로부터 1종 이상 선택된 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  17. 청구항 16에 있어서, 상기 분리주는 서열번호 45의 아미노산 서열을 포함하는 것인, 돼지 유행성 설사병 바이러스 (Porcine Epidemic Diarrhea Virus, PEDV) 대한민국 분리주.
  18. 청구항 1 내지 17 중 어느 한 항의 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 백신 조성물.
  19. 청구항 18에 있어서, 상기 백신은 생균 백신 또는 사균 백신인 것인, 백신 조성물.
  20. 청구항 1 내지 17 중 어느 한 항의 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물.
  21. 청구항 1 내지 17 중 어느 한 항의 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물.
  22. 청구항 1 내지 17 중 어느 한 항의 바이러스, 상기 바이러스의 배양물 또는 이의 항원을 검출할 수 있는 제제를 유효성분으로 포함하는 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 진단용 조성물.
  23. 청구항 1 내지 17 중 어느 한 항의 바이러스 생장 억제제를 포함하는, 돼지 유행성 설사병 (Porcine Epidemic Diarrhea, PED) 예방 또는 치료용 약학적 조성물.
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KR20190008508A (ko) * 2017-07-16 2019-01-24 주식회사 중앙백신연구소 신규 약독화 pedv 균주 및 이를 포함하는 백신 조성물
KR20200121447A (ko) * 2019-04-16 2020-10-26 주식회사 바이오앱 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법

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KR100267745B1 (ko) * 1998-01-10 2000-11-01 . 돼지 유행성설사병 바이러스의 특이 중화항체 검출방법
KR20030012280A (ko) * 2001-07-31 2003-02-12 주식회사 바이오비전텍 돼지 유행성 설사병 바이러스에 대한 백신효과를 유도하는형질전환 감자 및 이의 제조방법
KR100492820B1 (ko) * 2002-04-16 2005-05-31 양문식 돼지 유행성 설사병 바이러스 중화 에피토프 및 이의 이용
KR100713712B1 (ko) * 2005-10-28 2007-05-07 한국생명공학연구원 돼지 유행성 설사병 예방용 항원 단백질을 발현하는형질전환 식물체 및 이의 제조방법
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KR20190008508A (ko) * 2017-07-16 2019-01-24 주식회사 중앙백신연구소 신규 약독화 pedv 균주 및 이를 포함하는 백신 조성물
KR20200121447A (ko) * 2019-04-16 2020-10-26 주식회사 바이오앱 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법

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