KR20160013219A - 유전적 불임 동물 - Google Patents
유전적 불임 동물 Download PDFInfo
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- KR20160013219A KR20160013219A KR1020157037273A KR20157037273A KR20160013219A KR 20160013219 A KR20160013219 A KR 20160013219A KR 1020157037273 A KR1020157037273 A KR 1020157037273A KR 20157037273 A KR20157037273 A KR 20157037273A KR 20160013219 A KR20160013219 A KR 20160013219A
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| KR102285906B1 (ko) | 2021-02-18 | 2021-08-04 | 주식회사 엔에이피 | 수생태계 교란어종 관리를 위한 큰입배스 불임 유도용 조성물 |
| KR20250033452A (ko) | 2023-08-30 | 2025-03-10 | 서울대학교산학협력단 | Cyp17a1 형질전환 비만 및 불임치료제 개발 모델 제조방법 및 용도 |
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| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| CN105814214A (zh) | 2013-10-25 | 2016-07-27 | 家畜改良有限公司 | 遗传标记和其用途 |
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| US20170142942A1 (en) | 2014-07-14 | 2017-05-25 | Washington State University | Nanos knock-out that ablates germline cells |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| JP6851302B2 (ja) | 2015-04-08 | 2021-03-31 | 全国農業協同組合連合会 | 異個体由来の配偶子を生産する非ヒト大型哺乳動物又は魚類の作出方法 |
| WO2016196887A1 (en) | 2015-06-03 | 2016-12-08 | Board Of Regents Of The University Of Nebraska | Dna editing using single-stranded dna |
| WO2017062756A1 (en) * | 2015-10-07 | 2017-04-13 | Recombinetics, Inc. | Method of generating sterile terminal sires in livestock and animals produced thereby |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| CN105543257A (zh) * | 2016-01-18 | 2016-05-04 | 安徽农业大学 | 一种pAPN基因定点修饰猪 |
| KR102827276B1 (ko) | 2016-08-03 | 2025-07-01 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| CN110214180A (zh) | 2016-10-14 | 2019-09-06 | 哈佛大学的校长及成员们 | 核碱基编辑器的aav递送 |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510038A (ja) | 2017-03-09 | 2020-04-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | がんワクチン |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| GB2575930A (en) | 2017-03-23 | 2020-01-29 | Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| EP3498096A1 (en) * | 2017-12-18 | 2019-06-19 | IMV Technologies | Method for increasing the lifespan and motility of animal sperm using an inhibitor of slo3 potassium channel |
| CN110184296B (zh) * | 2018-02-07 | 2023-06-09 | 上海大学 | 一种制备雌雄均高度不育的鳞翅目昆虫的方法及其核酸构建物 |
| AU2019223203A1 (en) * | 2018-02-26 | 2020-09-10 | Aggenetics, Inc. | Materials and methods for preventing transmission of a particular chromosome |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2020047514A2 (en) * | 2018-08-31 | 2020-03-05 | Recombinetics, Inc. | Production method for animal models with disease associated phenotypes |
| GB201816633D0 (en) * | 2018-10-12 | 2018-11-28 | Univ Edinburgh | Genetically modified avians and method for the reconstitution thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| CN109837347B (zh) * | 2019-01-02 | 2021-01-29 | 华中农业大学 | Catsper4基因第三外显子的snp作为猪精液品质性状的遗传标记 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| NO20211349A1 (en) * | 2019-04-09 | 2021-11-09 | Mayo Found Medical Education & Res | Sterile fish |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN111378663B (zh) * | 2020-03-18 | 2021-07-13 | 吉林省农业科学院 | lncRNA SFR1及其应用、调控卵泡发育的产品和方法 |
| AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CN111549032A (zh) * | 2020-05-26 | 2020-08-18 | 肇庆学院 | 一种诱导型精原细胞Tubb4b基因敲除细胞系的构建方法 |
| TW202241259A (zh) * | 2020-12-31 | 2022-11-01 | 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） | 不育禽類胚胎、其產生及用途 |
| IL282597B (en) * | 2021-04-22 | 2022-08-01 | B G Negev Technologies And Applications Ltd At Ben Gurion Univ | Birds for producing female hatchling and methods of producing same |
| CN113558012A (zh) * | 2021-08-05 | 2021-10-29 | 陈米米 | 一种哺乳动物繁育雌性多胎的方法 |
| WO2024042515A1 (en) * | 2022-08-22 | 2024-02-29 | Ramot At Tel-Aviv University Ltd. | Genetically engineered eukaryotic organisms, systems and methods for in vivo biasing sex-ratio of populations |
| CN116064541B (zh) * | 2022-10-21 | 2025-03-18 | 华南农业大学 | 一种基于CRISPR/Cas9系统敲除猪细胞Y染色体的方法 |
| CN116884488B (zh) * | 2023-09-07 | 2023-11-24 | 中国医学科学院基础医学研究所 | 一种男性不育标志物的筛查方法、系统及设备 |
| CN118910165A (zh) * | 2024-10-11 | 2024-11-08 | 上海南方模式生物科技股份有限公司 | 一种cxcl16基因人源化动物模型的构建方法及应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2368620A1 (en) * | 1999-05-13 | 2000-11-23 | Cedars-Sinai Medical Center | Genetic modification of male germ cells for generation of transgenic species and genetic therapies |
| BR0108697A (pt) * | 2000-02-24 | 2005-01-11 | Univ Massachusetts Public Inst | Produção de mamìferos que produzem progênie de um único sexo |
| CA2442019A1 (en) * | 2001-03-22 | 2002-10-03 | Infigen, Inc. | Sex-specific selection of sperm from transgenic animals |
| AU2003213813A1 (en) * | 2002-03-22 | 2003-10-13 | St. Jude Children's Research Hospital | Improved method for generating genetically modified animals |
| EP2369916A4 (en) * | 2008-12-01 | 2013-04-10 | Univ Texas | PREPARATION AND USE OF SPERMATOGONIAL STEM CELL LINES FROM THE RAT |
| JP2013500018A (ja) * | 2009-07-24 | 2013-01-07 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | ゲノム編集のための方法 |
| EP2470557A1 (en) * | 2009-08-28 | 2012-07-04 | Novartis Forschungsstiftung, Zweigniederlassung Friedrich Miescher Institute For Biomedical Research | Genetic vasectomy by overexpression of prml-egfp fusion protein in spermatids |
| MX363013B (es) * | 2011-02-25 | 2019-03-04 | Recombinetics Inc | Animales genéticamente modificados y métodos para su obtención. |
| WO2012158985A2 (en) * | 2011-05-17 | 2012-11-22 | Transposagen Biopharmaceuticals, Inc. | Methods for site-specific genetic modification in spermatogonial stem cells using zinc finger nuclease (zfn) for the creation of model organisms |
| MX2015005255A (es) * | 2012-10-30 | 2015-10-29 | Recombinetics Inc | Control de la maduracion sexual en animales. |
| US20140359795A1 (en) * | 2013-05-31 | 2014-12-04 | Recombinetics, Inc. | Genetic techniques for making animals with sortable sperm |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102285906B1 (ko) | 2021-02-18 | 2021-08-04 | 주식회사 엔에이피 | 수생태계 교란어종 관리를 위한 큰입배스 불임 유도용 조성물 |
| KR20250033452A (ko) | 2023-08-30 | 2025-03-10 | 서울대학교산학협력단 | Cyp17a1 형질전환 비만 및 불임치료제 개발 모델 제조방법 및 용도 |
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| US20140359796A1 (en) | 2014-12-04 |
| RU2015156801A (ru) | 2017-07-06 |
| BR112015029904A2 (pt) | 2017-09-26 |
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| WO2014193583A3 (en) | 2015-01-22 |
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| AU2014272106A1 (en) | 2016-01-21 |
| CA2913411A1 (en) | 2014-12-04 |
| CN105473714A (zh) | 2016-04-06 |
| PH12015502640A1 (en) | 2016-03-07 |
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