KR20150079177A - Functional Cosmetic Composition Comprising Vitamin C Derivatives - Google Patents

Abstract

The present invention provides a whitening cosmetic composition which comprises: 100 to 200 parts by weight of a chestnut skin extract; 100 to 200 parts by weight of a broccoli extract; 100 to 200 parts by weight of a rosemary extract; 30 to 100 parts by weight of a morus bark extract; 200-600 parts by weight of a vitamin C derivative; and 200 to 300 parts by weight of an arbutin, with respect to 100 parts by weight of a licorice extract. The cosmetic composition of the present invention does not cause skin irritation and exhibits a remarkable whitening effect. In addition, the cosmetic composition of the present invention suppresses a tyrosinase enzyme which is a key enzyme in a melanin production mechanism and exhibits a remarkable whitening effect by suppressing a melanin generation quantity.

Description

[Technical Field] The present invention relates to a functional cosmetic composition containing a vitamin C derivative,

More particularly, the present invention relates to a functional cosmetic composition comprising vitamin C derivatives, and more particularly, to a cosmetic composition containing a vitamin C derivative, which comprises albutin, licorice extract and chestnut extract inhibiting tyrosinase activity, a vitamin C derivative which blocks melanin synthesis, The present invention relates to a functional cosmetic composition comprising adenosine.

The domestic cosmetics market is steadily growing, especially in the functional cosmetics market. Accordingly, it is required to develop functional cosmetics with high efficacy, which are upgraded with raw materials and functions that give higher added value in accordance with consumer needs.

One of the most important roles of functional cosmetics is how to get the ingredients that are useful to the skin to reach the desired part of the skin. No matter how expensive cosmetics are applied, it is of no use if the ingredients are not properly absorbed into the skin. In order for cosmetics to function properly, it is important to increase the skin absorbency of cosmetic ingredients. Therefore, it is necessary to develop a skin active substance based on the basic mechanisms of skin physiology and to develop appropriate products that can effectively transmit it. In general, the human skin has a barrier zone to protect itself from external stimuli or substances. Water-soluble substances are not allowed to enter the skin well, and no matter how effective the ingredients are, cosmetics can not achieve satisfactory results in skin improvement. The barrier zone also plays a very large role in the transfer of materials with large molecular weights. Therefore, it is important to develop the technology related to the percutaneous absorption system that appropriately transfers the efficacious substance into the skin of functional cosmetics.

A prodrug is a drug that can be easily absorbed by modifying the chemical structure of a drug that is difficult to absorb by the drug itself and then bioconverted to the original drug by enzymatic or hydrolysis in the body. . In other words, when the parent drug is not well absorbed due to poor distribution of the keratinocytes, the distribution can be increased by simple chemical modification such as attaching a high-fat substituent. After being absorbed into the skin, the prodrug is quickly metabolized and converted into the active drug while passing through the skin.

On the other hand, another important factor that functional cosmetics should have is an excellent whitening effect, which is closely related to the production of melanin. Melanin is produced by sunlight (ultraviolet rays) in melanocytes after tyrosine is converted to dopa and dopa chrome by an enzyme called tyrosinase and then through complex oxidation and condensation. The produced melanin is transferred to the skin cells and melanin is lost with epidermal detachment, and it is circulated to disappear. The mechanism of melanin production is characterized by the fact that only one enzyme called tyrosinase is involved. Therefore, whitening effect can be expected by inhibiting tyrosinase activity and preventing melanin production.

The method of inhibiting melanin production in whitening cosmetics can be largely divided as follows. The first method is to block and remove ultraviolet light which is a main cause of melanin production, and this method can effectively inhibit melanin production by containing a light scattering agent or a light blocking agent in a cosmetic composition. The second way is to inhibit the production of melanin by inhibiting the synthesis of core carbohydrates required for tyrosinase activity such as glucosamine. The third method is to interfere with the function of tyrosinase involved in melanogenesis, such as enzymatic acid (kojic acid) or arbutin. The fourth method has specific toxicity to melanocytes, such as hydroquinone, which produces melanin, which interferes with cell division. The fifth method is to expect a whitening effect by exerting a strong antioxidative effect while tyrosine, such as vitamin C, undergoes complex oxidation and condensation reaction through dopa and dopachrome.

In recent years, whitening cosmetics, which are considered to be most ideal, firstly block ultraviolet rays, eliminate radicals generated in the body, inhibit the production of inflammatory substances that may be caused by ultraviolet rays, and ultimately inhibit melanin biosynthesis It is known to use complex materials.

In connection with the combined use of substances having an effect of inhibiting melanin biosynthesis, Korean Patent Laid-Open No. 10-2009-0132284 (title of the invention: cosmetic composition for skin whitening containing a whitening complex) , Sasa extract, niacinamide, papain and Nopal extract.

In addition, in KR Patent Publication No. 10-2011-0012771 (title of the invention: extract of herbal medicines for skin whitening, methods for producing the same, and whitening cosmetic compositions containing them), whitening cosmetic compositions containing ear skin, ≪ / RTI >

However, in the case of the above-mentioned technical literatures, there is a problem that the whitening effect on bittern is excellent, but the whitening effect of the whitening substance is poor and the actual non-compensation whitening effect is not high.

KR 10-2009-0132284 A KR 10-2011-0012771 A

The inventors of the present invention have made efforts to develop whitening cosmetics having a high whitening effect and a low skin irritation degree as compared with conventional whitening cosmetics. As a result, it has been found that when a vitamin C derivative and a plurality of selected plant extracts are mixed, tyrosinase enzyme which is an important enzyme for melanogenesis is suppressed and whitening effect is suppressed by suppressing melanin production, thus completing the present invention .

Accordingly, it is an object of the present invention to provide a whitening cosmetic composition comprising a vitamin C derivative and a plurality of selected plant extracts.

Another object of the present invention is to provide a functional cosmetic composition which further comprises adenosine in the whitening cosmetic composition described above.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention provides a cosmetic composition.

The inventors of the present invention have made efforts to develop whitening cosmetics having a high whitening effect and a low skin irritation degree as compared with conventional whitening cosmetics. As a result, it was confirmed that when mixed with vitamin C derivatives and selected plant extracts, tyrosinase enzyme which is an important enzyme for melanogenesis is inhibited and whitening effect is suppressed by inhibiting melanin biosynthesis.

According to one embodiment of the present invention, 100-200 parts by weight of the extract of night bark, 100-200 parts by weight of broccoli extract, 100-200 parts by weight of rosemary extract, 100-300 parts by weight of a vitamin C derivative, and 100-300 parts by weight of arbutin.

As used herein, the term " golden " may refer to a medicament made from the roots of Scutellaria baicalensis GEORGE, a perennial herbaceous plant belonging to the family Lamiaceae.

As used herein, the term licorice may mean the roots and stems of licorice (Glycyrrhiza uralensis Fischer), European licorice (Glycyrrhiza glabra L.) or other inbred plants.

As used herein, the term " Rosmarinus " may refer to a perennial plant belonging to the family Lamiaceae.

As used herein, the term " Paeonia suffruticosa " may mean dicotyledonous plant buttercups and deciduous shrubs.

As used herein, the term " mulberry leaf " may mean a medicinal product made from the root bark of Morus alba L. or the plant of the same species.

As used herein, the term " vitamin C derivative " may mean a compound obtained by replacing part of the chemical structure of a vitamin C compound with another atom or atomic group.

According to a preferred embodiment of the present invention, the gold of the present invention can be preferably selected from the group comprising leaves, stems, fruits, roots and outposts of golden, more preferably stems or roots, Preferably a root.

The term " extract " used in reference to a plant extract in the present specification refers to an extract obtained by treating the above-mentioned plant leaves and / or stem with an extraction solvent, as well as the leaves and / But also includes formulated (e.g., powdered) workpieces.

If the extract used in the composition of the present invention is obtained by treating the leaves and / or stems of the plant with an extraction solvent, various extraction solvents may be used. (B) an anhydrous or a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol, (E) ethyl acetate, (f) chloroform, (g) butylene glycol, (h) hexane, (i) diethyl ether, or (j) butyl The extract can be obtained by treating the plant with acetate, more preferably water, ethanol or butylene glycol, to obtain the extract. More preferably, the extract is obtained by treating the plant with ethanol or butylene glycol, And most preferably ethanol, in the case of licorice, broccoli, chestnut, and rosemary, and in the case of the golden, peony root and bark, the butylene glycol may be treated to obtain an extract.

As a result of the whitening activity test, the whitening activity of extracts of licorice, broccoli, chestnut and rosemary was highest when ethanol extract was used. The highest whitening activity was obtained when the extract was treated with butylene glycol.

According to a preferred embodiment of the present invention, the whitening cosmetic composition of the present invention may further contain, as a preservative, 100-200 parts by weight of the gold extract and 50-100 parts by weight of peony root extract. In the case of the golden and peony root extracts, when it is included in the whitening cosmetic composition, it is possible to improve the preservation period of the product owing to the excellent antioxidative effect and to reduce the skin troubles which may be caused by addition of a chemical preservative have.

In addition, it was confirmed that the golden and peony root extracts significantly increased the whitening effect as compared with the case of not including them.

As used herein, the term " extract " has the meaning conventionally used in the art as a crude extract, but broadly includes fractions obtained by further fractionation of the extract. That is, the leaf and / or stem extracts of plants include not only those obtained by using the above-mentioned extraction solvent but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the above extract through an ultrafiltration membrane having a constant molecular weight cut-off value, and a separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) The fraction obtained by the purification method is also included in the leaf and / or stem extract of the plant of the present invention.

The leaves and / or stem extracts of plants used in the present invention may be prepared in powder form by further processes such as vacuum distillation and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the amount of the whitening cosmetic composition is preferably 0.00002-30% by weight, more preferably 0.0001-15.0% by weight, and most preferably 0.0025-10.0% by weight, Lt; / RTI >

In the composition of the present invention, the most preferable amount of the whitening cosmetic composition is 0.0025-10.0% by weight. When the content of the whitening cosmetic composition is less than 0.0025% by weight, the effect of the invention as a skin whitening cosmetic can not be sufficiently obtained If the content is more than 10.0% by weight, there is a problem in that the efficiency relative to the content is decreased because the degree of saturation of the effect is shown.

Therefore, since the content ratio of the whitening cosmetic composition in the composition of the present invention has a critical meaning, it should be used within the above-mentioned content ratio.

According to a preferred embodiment of the present invention, the vitamin C derivative of the present invention can be preferably in the form of magnesium salt of provitamin C or sodium salt of provitamin C, most preferably in the form of magnesium salt of provitamin C .

As can be seen from FIG. 1, the skin permeability was highest in the case of the magnesium salt form of provitamin C or the sodium salt form of provitamin C among the many vitamin C derivatives.

According to a preferred embodiment of the present invention, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray , And most preferably may be selected from the group comprising softening longevity, nutritional lotion, essence and nutritional cream.

 The cosmetic composition of the present invention may contain other ingredients besides the above-mentioned whitening cosmetic composition. According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

According to still another aspect of the present invention, there is provided a method for preparing a herbal composition comprising 100-200 parts by weight of an extract of night bark, 100-200 parts by weight of a broccoli extract, 100-200 parts by weight of a rosemary extract, 100-300 parts by weight of vitamin C derivatives, 100-300 parts by weight of arbutin and 100-300 parts by weight of adenosine.

Since the whitening and wrinkle improving cosmetic composition is common to the whitening cosmetic composition as described above except that adenosine is additionally added to the whitening cosmetic composition as a wrinkle improving substance, .

Meanwhile, as a result of cell viability analysis of the active ingredient of the composition of the present invention, it was found that the active ingredient of the composition of the present invention is a harmless substance as a natural substance. Therefore, since the active ingredient of the present invention has little toxicity and side effects, it can be safely used even in long-term use, and can be safely applied to the cosmetic composition as described above.

The features and advantages of the present invention are summarized as follows:

(a) 100-200 parts by weight of a chestnut bark extract, 100-200 parts by weight of a broccoli extract, 100-200 parts by weight of a rosemary extract, 30-100 parts by weight of an extract of mulberry bark, 30-100 parts by weight of a vitamin C derivative 100-300 parts by weight of arbutin and 100-300 parts by weight of arbutin.

(b) The cosmetic composition of the present invention exhibits a remarkable whitening effect without skin irritation.

(c) The cosmetic composition of the present invention suppresses the tyrosinase enzyme, which is an enzyme important for the melanin production mechanism, and suppresses the amount of melanin production, thereby exhibiting a remarkable whitening effect.

Figure 1 relates to skin permeability experimental results of various vitamin C derivatives.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

Example

Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.

Manufacturing example

Production Example 1: Preparation of plant extract

Production Example 1-1: Preparation of licorice ethanol extract

1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added to 100 g of dried licorice and extracted at room temperature for 3 days. The licorice was filtered with a 380 mesh filter cloth, and the filtrate was collected. The remaining licorice was added with the same amount of 70% ethanol and extracted at room temperature for 3 days. The licorice was filtered with 380 mesh filter cloth and the filtrate was collected. The filtrate was filtered with a 0.45 ㎛ membrane filter together with the filtrate collected in the previous filtration, and the filtrate was concentrated under reduced pressure. The final extract was obtained through lyophilization.

Production Example 1-2: Preparation of chestnut ethanol extract

1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added to 100 g of the dried night skin and extracted at room temperature for 3 days. The night bark was filtered with a 380 mesh filter cloth, and the filtrate was collected. Again, the chestnut shells were extracted with the same amount of 70% ethanol and extracted at room temperature for 3 days. The chestnuts were filtered with a 380 mesh filter cloth and the filtrate was collected. The filtrate was filtered with a 0.45 ㎛ membrane filter together with the filtrate collected in the previous filtration, and then the filtrate was concentrated under reduced pressure The final extracts were obtained through concentration and lyophilization.

Production Example 1-3: Preparation of ethanol extract of broccoli

1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added to 100 g of dried broccoli and extracted at room temperature for 3 days. The broccoli was filtered with a 380 mesh filter cloth, and the filtrate was collected. The broccoli was extracted with the same amount of 70% ethanol for 3 days at room temperature, broth was filtered with a 380 mesh filter, and the filtrate was collected. The filtrate was filtered with a 0.45 ㎛ membrane filter together with the filtrate collected in the previous filtration, and the filtrate was concentrated under reduced pressure. The final extract was obtained through lyophilization.

Production Example 1-4: Preparation of Rosemary Ethanol Extract

1500 g of 70% ethanol (sample: solvent = 1: 15 wt / wt) was added to 100 g of dried rosemary and extracted at room temperature for 3 days. The rosemary was filtered with a 380 mesh filter cloth, and the filtrate was collected. The filtered rosemary was extracted with the same amount of 70% ethanol, extracted at room temperature for 3 days, rosemary was filtered with a 380 mesh filter, and the filtrate was collected. The filtered rosemary was filtered with a 0.45 ㎛ membrane filter with a filtration apparatus under reduced pressure, and the filtrate was concentrated under reduced pressure. The final extract was obtained through lyophilization.

Production Example 1-5: Preparation of Golden Butylene Grignol Extract

500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) was added to 20 g of dried gold outpowder, and the mixture was extracted at room temperature for 5 days. After 5 days, the golden outpost was filtered with a 380 mesh filter cloth, filtered through a 0.45 ㎛ membrane filter, and the final extract was obtained.

Production Example 1-6: Preparation of peony root butylene glycol extract

500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) was added to 20 g of dried peony root and the mixture was extracted at room temperature for 5 days. After 5 days, the peony root was filtered with 380 mesh filter cloth, filtered through a 0.45 μm membrane filter, and the final extract was obtained.

Production Example 1-7: Preparation of Butyleneglycol Extract of Eucalyptus

500 g of 30% butylene glycol (sample: solvent = 1: 25 wt / wt) was added to 20 g of dried corn syrup and extracted at room temperature for 5 days. After 5 days, the alfalfa was filtered with a 380 mesh filter cloth, filtered through a 0.45 μm membrane filter, and then the final extract was obtained.

Preparation Example 2: Preparation of complex whitening composition and complex functional composition

100 parts by weight of the bark extract, 100 parts by weight of the broccoli extract, 100 parts by weight of the rosemary extract, 60 parts by weight of the extract of the manganese bark extract, APM of the vitamin C derivative (Mg of the provitamin C Salt) and 600 parts by weight of arbutin were mixed to prepare the complex whitening cosmetic composition of Example 1. In addition, 400 parts by weight of adenosine was further added to the complex whitening composition of Example 1 based on 100 parts by weight of the licorice extract to prepare a complex functional cosmetic composition of Example 2.

Prescription example

Prescription example  1: Complex functionality Cosmetics  Preparation of softening water containing composition

Examples of the formulation of the softening water in cosmetics containing the multi-functional cosmetic composition of Example 2 are as follows.

Preparation of softening water containing complex functional cosmetic composition ingredient Content (% by weight) Material No. Example 0.25 Polyoxyethylene hardened castor oil 0.5 One Glycine 3.0 2 Dipotassium glycyrrhizate 0.1 3 1,3-butylene glycol 3.0 4 Sodium hyaruronate 0.1 5 ethanol 5.0 6 Antioxidant 0.1 7 Triethanolamine 0.1 8 EDTA 0.1 9 antiseptic Suitable amount 10 Purified water Balance 11

Sodium hyaruronate was prepared by dispersing the purified water in a propeller mixer at 3000 rpm to prepare a 1% solution. The raw material 1-8 was homogenized in a propeller mixer at 500 rpm and completely dissolved by heating at 75 DEG C, and then cooled to room temperature. The raw material 9-10 was completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank, followed by stirring and mixing. Example 2 was added thereto and thoroughly stirred and mixed to prepare a softening liquid containing Example 2.

Prescription Example 2: Preparation of a nutritional lotion containing a complex functional cosmetic composition

Examples of the prescription of the nutritional lotion among the cosmetic compositions containing the multi-functional cosmetic composition of Example 2 are as follows.

Preparation of nutritional lotion containing complex functional cosmetic composition ingredient Content (% by weight) Material No. Example 2 1.0 glycerin 7.0 One Sorbitan stearate sucrose cocoate 2.0 2 Mineral oil 4.0 3 Trioctanoin 1.0 4 Stearic Acid 1.0 5 Glyceryl stearate 0.5 6 Sorbitan monostearate 1.0 7 Dimethicone 0.5 8 Antioxidant 0.3 9 Triethanolamine 0.1 10 Carbomer 0.2 11 EDTA 0.1 12 antiseptic Suitable amount 13 Purified water Balance 14

The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. The raw material 1-7 was added to the water dissolving tank and homogenized at 2000 rpm for dispersion, and then heated to 75 캜. Ingredients 8-13 were added to the oily melting tank and dissolved by heating to 75 ° C. The oil phase dissolved in an aqueous dissolution tank was charged and emulsified (3000 rpm / 5 minutes) and then cooled to room temperature. Example 2 was added thereto and thoroughly stirred and mixed to prepare a nutrition lotion containing Example 2.

Prescription Example 3: Preparation of Essence Containing Complex Functional Cosmetic Composition

Examples of prescriptions of essences in cosmetics containing the multifunctional cosmetic composition of Example 2 are as follows.

Preparation of Essence Containing Multifunctional Cosmetic Composition of Example 2 ingredient Content (% by weight) Material No. Example 2 3.0 glycerin 5.0 One 1,3-butylene glycol 2.0 2 Polyethylene glycol 2.0 3 Carbomer 1.0 4 Sodium hyaruronate 0.1 5 Glycine 3.0 6 Polyacrylamide 2.0 7 Hydroxyethyl cellulose 0.2 8 ethanol 3.0 9 Antioxidant 0.3 10 Triethanolamine 0.1 11 Polyoxyethylene hardened castor oil 1.0 12 EDTA 0.1 13 antiseptic Suitable amount 14 Purified water Balance 15

Sodium hyaruronate and hydroxyethyl cellulose were dispersed in purified water in a propeller mixer at 2000 rpm to prepare a 1% solution. The carbomer was prepared by dispersing the purified water in a propeller mixer at 4000 rpm in a 2% solution. The raw material 1-12 was added to the water dissolution tank, homogenized at 2000 rpm, dispersed, and then heated to 75 캜. The warmed water phase was cooled to room temperature. The raw materials 13 to 14 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. Example 2 was added thereto and thoroughly stirred and mixed to prepare an essence containing Example 12.

Prescription Example 4: Preparation of nutritional cream containing complex functional cosmetic composition

An example of the formulation of the nutritional cream in cosmetics containing the multi-functional cosmetic composition of Example 2 is as follows.

Preparation of a nutritional cream containing the complex functional cosmetic composition of Example 2 ingredient Content (% by weight) Material No. Example 2 10.0 1,3-butylene glycol 3.0 One glycerin 3.0 2 Hydrogenated lecithin 1.0 3 Octyldodecanol 3.0 4 Trioctanoin 2.0 5 Stearic Acid 1.5 6 Cetostearyl alcohol 2.0 7 Polysorbate 60 1.5 8 Sorbitan sesquioleate 2.0 9 Dimethicone 3.0 10 Antioxidant 0.3 11 Xanthan gum 0.2 12 Triethanolamine 0.1 13 EDTA 0.1 14 antiseptic Suitable amount 15 Purified water Balance 16

The raw material 1-8 was added to the water dissolution tank and homogenized at 2000 rpm with stirring, and then heated to 75 캜. The raw material 9-15 was added to the oily melting tank and dissolved by heating to 80 ° C. The oil phase dissolved in the water-dissolving tank was charged and emulsified (3000 rpm / 10 minutes) and then cooled to room temperature. Example 2 was added thereto and thoroughly stirred and mixed to prepare a nutritive cream containing Example 2.

Experimental Example

Experimental Example 1: Mushroom tyrosinase inhibitory effect on melanin formation

Experimental Example 1-1: Inhibition of Mushroom tyrosinase activity

The tyrosinase activity inhibitory potency of the complex whitening cosmetic composition of Example 1 was measured by modifying a method of Yagi et al. Which measures DOPA chrome produced as a result of the action of tyrosinase by the colorimetric method. That is, 40 μl of a 1.5 mM L-Tyrosine solution (0.1 M phosphate buffer (pH 6.5)) and 10 μl of a sample solution were added to 110 μl of a 0.1 M phosphate buffer solution (pH 6.5) in a 96-well plate, 10 μl of tyrosinase (2000 unit / ml) was added and reacted at 37 ° C for 10 minutes. Dopachrome generated in the reaction solution was measured at 490 nm using a microplate counter. Arbutin was used as a positive control for comparison of test results. The experimental results are shown in Table 5 below.

Mushroom tyrosinase activity inhibitory effect Arbutin Example 1 Samples (占 퐂 / ml) 500 100 Inhibitory effect of tyrosinase 43% 66%

Experimental Example 2: Melanin biosynthesis inhibition test for B16 melanoma

In order to measure the effect of suppressing the melanin biosynthesis in mouse derived B16F1 melanoma, the following experiment was conducted. B16F1 (melanoma) was cultured in 10% FBS-DMEM, and the cell concentration was adjusted to 5.0 × 10 ⁴ cells / ml. Cells were dispensed into 2-well plates at 6 ° C, in% CO ₂ incubator and incubated for 24 hours. After culturing for 24 hours, the medium was removed, and 1.8 ml of the medium prepared to a final concentration of 0.2 uM α-MSH and 200 μl of the sample of Example 1 were mixed and cultured for 72 hours under the same conditions. At this time, the positive control used the same concentration of arbutin. After culturing for 72 hours, 200 μl of Trypsine-EDTA solution was added to the plate, washed twice with 1 × PBS (-) to measure the amount of melanin in each solution, and the cells were separated and centrifuged And the supernatant was removed. The separated pellet is washed twice with 1 x PBS (-) and then dried at 70 ° C for 1 hour. 400 μl of 10% DMSO (in 1 M NaOH) was added and dissolved. 200 μl of each was dispensed into a 96-well plate, and absorbance was measured at 490 nm using a microplate counter. As a result, the melanin synthesis inhibition rate of the complex whitening cosmetic composition of Example 1 was 20%, which was higher than that of the control group, arbutin 500 ㎍ / ml 13%.

Experimental Example 3: Skin whitening effect test

In order to confirm that the whitening effect shown in the in-bit test actually contributes to skin whitening of human beings, a human body application test was conducted on the cream containing Example 2. The skin whitening test using Roboskin analyzer equipment periodically evaluated the improvement of pigmentation and skin tone (brightness) while applying samples or products to the subject's face. The test consisted of 10 subjects (5 control subjects, 5 test subjects), all of whom were female and the average age was 35. The mean values were obtained by measuring 5 times in total for 1 week, 2 weeks, 4 weeks and 6 weeks before use. All tests were conducted in a constant temperature and humidity chamber at 20-24 ° C and a humidity of 45-55% And then at least one hour in a humidity chamber.

Identification of whitening effect of nutritional cream prepared by the method of Prescription Example 4 Before use 1 week 2 weeks 4 weeks 6 weeks Control Test section Control Test section Control Test section Control Test section Control Test section Pigmentation (small) 9.2 8.9 9.1 8.2 9.1 7.7 8.8 6.1 8.2 4.1 Pigmentation (large) 33.0 31.9 31.7 30.4 31.0 29.7 29.7 26.9 29.1 22.9 brightness 64.1 65.8 65.9 67.8 65.7 70.9 66.9 74.5 68.7 77.8

As a result of measuring the skin whitening effect for Prescription Example 4, it was found that the prescription Example 4 also showed a tendency that the pigmentation (large and small) was decreased from the 2nd week and the brightness of the skin also became brighter, .

Claims (10)

Based on 100 parts by weight of licorice extract,
100-200 parts by weight of the bark extract, 100-200 parts by weight of the broccoli extract, 100-200 parts by weight of the rosemary extract, 30-100 parts by weight of the extract,
200-600 parts by weight of a vitamin C derivative and
200-600 parts by weight of arbutin. The method according to claim 1,
Wherein the extraction solvent of the extract is selected from the group consisting of water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, nucleic acid, ethanol, butylene glycol, chloroform and mixtures thereof. 3. The method of claim 2,
Wherein the extraction solvent of the extract is water or butylene glycol. The method according to claim 1,
Wherein the whitening cosmetic composition further comprises 100-200 parts by weight of a golden extract and 50-100 parts by weight of a peony root extract as a preservative. The method according to claim 1,
Wherein the vitamin C derivative is a magnesium salt form of provitamin C or a sodium salt form of provitamin C. 6. The method of claim 5,
Wherein the vitamin C derivative is a magnesium salt form of provitamin C. 7. The method according to any one of claims 1 to 6,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the cosmetic composition is a cosmetic composition. Based on 100 parts by weight of licorice extract,
100-200 parts by weight of the bark extract, 100-200 parts by weight of the broccoli extract, 100-200 parts by weight of the rosemary extract, 30-100 parts by weight of the extract,
100-300 parts by weight of a vitamin C derivative,
200-600 parts by weight of arbutin and
And 200-600 parts by weight of adenosine. 9. The method of claim 8,
Wherein the extracting solvent of the extract and the extract of Mulberry bark extract is butylene glycol. 9. The method of claim 8,
Wherein the extract of the licorice, chestnut shell and broccoli extract is ethanol.

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