KR20150002588A

KR20150002588A - 인터페론 및 면역글로불린 fc 섹션 융합 단백질

Info

Publication number: KR20150002588A
Application number: KR20147022960A
Authority: KR
Inventors: 티선-융 쿠오; 청-친 우; 한-팅 첸
Original assignee: 에스비씨 버락 바이오테크 코., 엘티디.
Priority date: 2012-01-19
Filing date: 2013-01-18
Publication date: 2015-01-07
Also published as: PH12014501643A1; WO2013107388A1; CN103214579A; CN105001340A; CN103214579B; BR112014017788A2

Abstract

본 발명은 동물 인터페론 및 동물 면역글로불린 Fc 섹션을 함유하는 융합 단백질을 제공한다. 융합 단백질은 인터페론 및 면역글로불린 Fc 섹션을 연결시키기 위한 링커를 추가로 포함한다. 본 발명은 융합 단백질을 인코딩하는 폴리리보뉴클레오티드, 융합 단백질을 제조하는 방법, 및 항바이러스 약물의 제조에서의 융합 단백질의 적용을 추가로 제공한다.

Description

인터페론 및 면역글로불린 FC 섹션 융합 단백질{INTERFERON AND IMMUNE GLOBULIN FC SECTION FUSION PROTEIN}

본 발명은 재조합 융합 인터페론, 특히 동물 바이러스에 대한 항바이러스 활성을 갖는 재조합 융합 인터페론에 관한 것이다.

발명의 배경

1. 발명의 분야

본 발명은 동물용 재조합 융합 인터페론, 특히 동물 바이러스에 대한 항바이러스 활성을 갖는 재조합 융합 인터페론에 관한 것이다.

2. 관련 분야의 설명

인터페론(IFN)은 인플루엔자 바이러스의 연구중에 앨릭 이삭스(Alick Isaacs) 및 장 린덴만(Jean Lindenmann)에 의해 1957년에 최초로 발견되었다. 바이러스에 의해 감염된 후, 숙주 세포는 가까이의 다른 세포를 유도하는 사이토카인을 즉시 방출하여, 바이러스 복제를 방해하는 항바이러스 단백질을 생성시킨다. 이러한 사이토카인은 이후에 IFN으로 명명되었다. IFN의 항바이러스 효과는 주로 타입 I IFN(IFN-α 및 IFN-β)에 의해 제공된다. 항바이러스 활성에 더하여, IFN은 항-종양 활성을 가지며, 세포 분화를 유도하고, 면역 반응을 조절할 수 있다.

지금까지, 상업적 IFN의 대부분은 인간 질병, 예를 들어, 인간 B형 간염, 인간 C형 간염, 카포시 육종(KS), 및 악성 흑색종의 치료에 사용되어 왔다.

동물 바이러스를 예방하기 위한 효과적인 백신 없이는, 바이러스에 감염된 동물은 지지 요법으로만 치료될 수 있다. 그러나, 지지 요법은 보통 비효과적이고, 따라서 동물 바이러스의 감염은 가축 사육에서 큰 경제적 손실을 초래할 수 있다. 따라서, 동물 바이러스에 대한 항바이러스 활성을 갖는 IFN을 개발하는 것이 중요하다.

발명의 개요

본 발명의 첫번째 양태는 동물용 재조합 융합 IFN에 관한 것이다. IFN은 이의 작은 분자 크기로 인해 짧은 혈청 반감기(약 2 내지 8시간)를 갖는다. 한 구현예에서, 본 발명은 동물 IFN이 긴 혈청 반감기를 갖는 동물 면역글로불린 Fc 단편(IgG Fc)과 융합된 안정적인 동물용 재조합 융합 IFN을 제공한다. 한 바람직한 구현예에서, 동물 IFN 및 동물 IgG Fc는 글리신 및 세린 잔기를 포함하는 펩티드 링커에 의해 연결된다.

또 다른 구현예에서, 동물 IFN은 돼지 IFNα이다. 돼지 IFNα는 SEQ ID NO:2, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 및 47 중 적어도 하나의 서열과 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 또는 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%의 상동성을 갖는 아미노산 서열을 인코딩한다. 한 바람직한 구현예에서, 돼지 IFNα는 SEQ ID NO:2, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 및 47로 구성된 군으로부터 선택된 적어도 하나의 서열을 인코딩한다.

또 다른 구현예에서, 동물 IgG Fc는 돼지 면역글로불린 Fc 단편이다. 돼지 IgG Fc는 SEQ ID NO:4, 49, 51, 53, 및 55 중 적어도 하나의 서열과 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 또는 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%의 상동성을 갖는 아미노산 서열을 인코딩한다. 한 바람직한 구현예에서, 돼지 IgG Fc는 SEQ ID NO:4, 49, 51, 53, 및 55로 구성된 군으로부터 선택된 적어도 하나의 서열을 인코딩한다.

또 다른 구현예에서, 펩티드 링커는 SEQ ID NO:6의 서열과 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 또는 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%의 상동성을 갖는 아미노산 서열을 인코딩한다. 한 바람직한 구현예에서, 펩티드 링커는 SEQ ID NO:6의 아미노산 서열을 인코딩한다.

한 추가 구현예에서, 동물용 재조합 융합 IFN은 SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223 중 적어도 하나의 서열과 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 또는 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%의 상동성을 갖는 아미노산 서열을 인코딩한다. 한 바람직한 구현예에서, 동물용 재조합 융합 IFN은 SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223으로 구성된 군으로부터 선택된 적어도 하나의 서열을 인코딩한다.

본 발명의 두번째 양태는 본 발명의 동물용 재조합 융합 IFN을 인코딩하는 폴리뉴클레오티드에 관한 것이다. 동물 IFN을 인코딩하는 DNA 서열 및 동물 IgG Fc를 인코딩하는 DNA 서열은 발현 벡터로 클로닝되어, 동물용 재조합 융합 IFN을 인코딩하는 폴리뉴클레오티드가 수득된다. 동물용 재조합 융합 IFN을 인코딩하는 폴리뉴클레오티드를 함유하는 벡터는 이후 숙주 세포로 도입되고, 여기서 상기 동물용 재조합 융합 IFN이 발현될 수 있다.

한 바람직한 구현예에서, 동물 IFN을 인코딩하는 DNA 서열 및 동물 IgG Fc를 인코딩하는 DNA 서열을 발현 벡터로 클로닝하는 것에 더하여, 글리신 및 세린 잔기를 갖는 펩티드 링커를 인코딩하는 DNA 서열이 발현 벡터로 추가로 클로닝되어, 동물 IFN을 인코딩하는 DNA 서열 및 동물 IgG Fc를 인코딩하는 DNA 서열이 연결된다.

한 구현예에서, 동물 IFN을 인코딩하는 DNA 서열은 SEQ ID NO:1, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 및 46의 DNA 서열 중 적어도 하나와 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%의 상동성을 갖는다. 한 바람직한 구현예에서, 동물 IFN을 인코딩하는 DNA 서열은 SEQ ID NO:1, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 및 46으로 구성된 군으로부터 선택된 적어도 하나의 DNA 서열이다.

한 구현예에서, 동물 IgG Fc를 인코딩하는 DNA 서열은 SEQ ID NO:3, 48, 50, 52, 및 54의 DNA 서열 중 적어도 하나와 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%의 상동성을 갖는다. 한 바람직한 구현예에서, 동물 IgG Fc를 인코딩하는 DNA 서열은 SEQ ID NO:3, 48, 50, 52, 및 54로 구성된 군으로부터 선택된 적어도 하나의 DNA 서열이다.

한 구현예에서, 글리신 및 세린 잔기를 갖는 펩티드 링커를 인코딩하는 DNA 서열은 SEQ ID NO:5의 DNA 서열과 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%의 상동성을 갖는다. 한 바람직한 구현예에서, 글리신 및 세린 잔기를 갖는 펩티드 링커를 인코딩하는 DNA 서열은 SEQ ID NO:5의 DNA 서열을 갖는다.

한 구현예에서, 동물용 재조합 융합 IFN을 인코딩하는 DNA 서열은 SEQ ID NO:7, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 및 222의 DNA 서열 중 적어도 하나와 적어도 80%, 바람직하게는 85%, 더욱 바람직하게는 90%, 더욱 더 바람직하게는 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%의 상동성을 갖는다. 한 바람직한 구현예에서, 동물용 재조합 융합 IFN을 인코딩하는 DNA 서열은 SEQ ID NO:7, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 및 222의 DNA 서열 중 하나를 갖는다.

발현 벡터는 원핵생물 발현 벡터 또는 진핵생물 발현 벡터일 수 있다. 원핵생물 발현 벡터는 pET, pGEX, 및 pDEST 발현 벡터를 포함하나, 이에 제한되지는 않는다. 진핵생물 발현 벡터는 pSecTag, pcDNA3, pCMV-Script, pCI, 및 pSV40b 발현 벡터를 포함하나, 이에 제한되지는 않는다.

숙주 세포는 원핵생물 세포, 예를 들어, 박테리아, 또는 진핵생물 세포, 예를 들어, 효모, 곤충 세포, 식물 세포, 및 포유동물 세포일 수 있다. 한 구현예에서, 숙주 세포는 에스케리키아 콜리(Escherichia coli)이다. 또 다른 구현예에서, 숙주 세포는 포유동물 세포이다. 본 발명의 동물용 재조합 융합 인터페론을 발현시키기 위해 사용될 수 있는 포유동물 세포는 3T3 세포, 차이니즈 햄스터(Chinese hamster) 난소 세포(CHO 세포), 새끼 햄스터 신장 세포(BHK 세포), 인간 자궁경부암 세포(예를 들어, Hela 세포), 및 인간 간 암종 세포(예를 들어, HepG2 세포)를 포함하나, 이에 제한되지는 않는다.

본 발명의 세번째 양태는 포유동물 숙주 세포 내에서 본 발명의 동물용 재조합 융합 인터페론을 생성시키기 위한 최적화된 방법에 관한 것이다. 한 구현예에서, 상기 방법은 (1) 혈청-함유 배지에서 본 발명의 동물용 재조합 융합 인터페론을 인코딩하는 폴리뉴클레오티드를 갖는 포유동물 숙주 세포를 배양하는 단계, (2) 세포가 안정적으로 성장한 후에 혈청-함유 배지를 혈청-비함유 배지로 대체하는 단계, (3) 1 내지 5일마다 본 발명의 동물용 재조합 융합 인터페론을 함유하는 혈청-비함유 배지를 수거하고, 새로운 혈청-비함유 배지를 세포에 첨가하는 단계를 포함한다.

포유동물 숙주 세포는 3T3 세포, 차이니즈 햄스터 난소 세포(CHO 세포), 새끼 햄스터 신장 세포(BHK 세포), 인간 자궁경부암 세포(예를 들어, Hela 세포), 및 인간 간 암종 세포(예를 들어, HepG2 세포)를 포함하나, 이에 제한되지는 않는다. 한 구현예에서, 포유동물 숙주 세포는 차이니즈 햄스터 난소 세포(CHO 세포)이다.

혈청은 소 혈청 및 말 혈청을 포함하나, 이에 제한되지는 않는다. 한 구현예에서, 혈청은 소 태아 혈청(FBS)이다. 배지 내의 혈청의 함량은 약 0.1 내지 10%(v/v)이다. 한 구현예에서, 혈청의 함량은 5%(v/v)이다.

본 발명의 네번째 양태는 동물용 항바이러스 약제의 제조를 위한 본 발명의 동물용 재조합 융합 인터페론의 용도에 관한 것이다. 본 발명에 제공된 시험은 본 발명의 동물용 재조합 융합 인터페론이 항바이러스 활성을 갖는 것을 나타낸다. DNA 바이러스(예를 들어, 거짓광견병(pseudorabies) 바이러스, PRV) 및 RNA 바이러스(예를 들어, 돼지 생식기 호흡기 증후군 바이러스, PRRSV)가 본 발명의 동물용 재조합 융합 인터페론의 항바이러스 활성을 평가하기 위해 각각 시험관내 실험에서 사용되었다. 결과는 본 발명의 동물용 재조합 융합 인터페론이 동물에서 바이러스 감염을 효과적으로 억제할 수 있고, 본 발명의 동물용 재조합 융합 인터페론이 동물용 비-융합 인터페론보다 큰 항바이러스 활성을 갖는 것을 나타낸다. 따라서, 본 발명의 동물용 재조합 융합 인터페론은 동물에서 바이러스 감염을 억제하는데 사용될 수 있다.

본원에 기재된 바와 같은 기술 및 과학 용어의 의미는 당업자에 의해 명백하게 이해될 수 있다.

본 발명은 하기 예시적 실시예에서 더욱 상세히 기재된다. 이러한 실시예는 단지 본 발명의 선택된 구현예만 기재하고 있으나, 하기 실시예는 예시적인 것으로 이로 제한되는 것이 아님이 이해되어야 한다.

도 1A는 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포의 면역형광 검정(IFA) 검정을 도시한다. 도 1B는 pSecTag2(B)로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포의 IFA 검정을 도시한다.
도 2는 본 발명의 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포, 및 pSecTag2(B)로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포의 ELISA 검정을 도시한다.
도 3은 본 발명의 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 포함하는 CHO 세포에서 발현된 단백질의 웨스턴 블롯을 도시한다. M: 단백질 마커; 레인 1: 단백질의 SDS-PAGE 분석; 레인 2: 마우스 항-IFNα 모노클로날 항체를 이용하여 검출된 웨스턴 블롯; 레인 3: 마우스 항-His 모노클로날 항체를 이용하여 검출된 웨스턴 블롯; 레인 4: 염소 항-돼지 IgG 항체를 이용하여 검출된 웨스턴 블롯.
도 4는 PRRSV 공격된 Marc-145 세포 및 PR 공격된 ST 세포에서의 본 발명의 돼지 재조합 융합 인터페론(P IFN-Fc 그룹) 및 돼지 면역글로불린 IgG-Fc 단편이 없는 돼지 인터페론(P IFN 그룹)에 대한 항바이러스 감염 검정의 결과를 도시한다.

바람직한 구현예의 상세한 설명

실시예 1 - 돼지 인터페론 α1의 분자 클로닝

말초 혈액 단핵 세포(PBMC)를 먼저 돼지(L×Y-D 계통)의 혈액으로부터 분리시켰다. 전체 RNA를 구아니딘 티오시아네이트(GTC) 방법에 의해 PBMC로부터 분리시킨 후, 역전사 중합효소 연쇄 반응(RT-PCT)에 사용하여 상보성 DNA(cDNA)를 생성시켰다. 간단히, 20 ㎕의 전체 RNA를 3분 동안 70℃에서 인큐베이션하고, 인큐베이션 후, 10 ㎕의 5x 완충액, 8 ㎕의 1.25mM dNTP, 1 ㎕의 올리고 dT 프라이머, 11 ㎕의 증류수, 0.5 ㎕의 RNasin, 및 0.5 ㎕의 AMV 역전사효소를 첨가하고, 30분 동안 42℃에서 인큐베이션하여 cDNA를 합성하였다. 이후, 합성된 cDNA를 DNA 주형으로 사용하여, 중합효소 연쇄 반응(PCR)에 의해 돼지 인터페론 α1(P IFNα1) 유전자를 클로닝하였다. 10 ㎕의 cDNA, 5 ㎕의 10x PCR 완충액, 8 ㎕의 1.25 mM dNTP, 1 ㎕의 정방향 프라이머, 1 ㎕의 역방향 프라이머, 24 ㎕의 가압증기멸균된 물, 및 1 ㎕의 Taq 중합효소를 함유하는 PCR 혼합물을 GeneAmp® PCR System 2400 반응기(Applied Biosystems)에 두었다. PCR 프로그램은 5분 동안 95℃의 1 주기, 95℃/1분, 55℃/30초, 72℃/30초의 30주기 후, 72℃/5분의 인큐베이션이었다. P IFNα1을 클로닝하기 위한 프라이머는 다음과 같다:

PCR 생성물의 크기를 아가로스 전기영동에 의해 검출하였다. 이후, PCR 생성물을 DNA 정제 키트(Protech Technology Enterprise, Taiwan)로 정제하였다. 정제 후, PCR 생성물을 pET20b 발현 벡터에 작제하였다. 정제된 PCR 생성물 및 pET20b 발현 벡터를 2개의 제한 효소 HindIII 및 Xho I로 각각 절단하였다. 제한 효소 절단 반응 후, 절단된 PCR 생성물 및 pET20b 발현 벡터를 DNA 정제 키트(Protech Technology Enterprise, Taiwan)로 각각 정제하였다. 정제된 PCR 생성물을 정제된 pET20b 발현 벡터와 라이게이션시켜 플라스미드 pET20b-IFNα1을 형성시켰고, 플라스미드를 숙주 세포(E. coli)에 형질전환시켰다. 형질전환주를 선택하고, 돼지 인터페론 α1(P IFNα1)의 DNA 서열을 DNA 시퀀싱에 의해 확인하였다. 돼지 인터페론 α1(P IFNα1)은 SEQ ID NO:1의 DNA 서열 및 SEQ ID NO:2의 아미노산 서열을 갖는다.

실시예 2 - 돼지 면역글로불린 Fc 4α 단편의 분자 클로닝

전체 RNA를 GTC 방법에 의해 돼지 비장으로부터 분리시킨 후, RT-PCR에서 사용하여 cDNA를 생성시켰다. 간단히, 20 ㎕의 전체 RNA, 8 ㎕의 1.25mM dNTP, 및 1 ㎕의 올리고 dT 프라이머를 5분 동안 70℃에서 인큐베이션하고, 인큐베이션 후, 1 ㎕의 가압증기멸균된 물, 8 ㎕의 5xAMV RT 완충액, 1 ㎕의 RNasin, 및 1 ㎕의 AMV 역전사효소를 첨가하고, 1시간 동안 42℃에서 인큐베이션하여 cDNA를 합성하였다. 이후, 합성된 cDNA를 PCR에서 DNA 주형으로 사용하였다. 1 ㎕의 cDNA, 5 ㎕의 10x PCR 완충액, 8 ㎕의 1.25 mM dNTP, 1 ㎕의 정방향 프라이머, 1 ㎕의 역방향 프라이머, 33 ㎕의 가압증기멸균된 물, 및 1 ㎕의 Taq 중합효소를 함유하는 PCR 혼합물을 GeneAmp® PCR System 2400 반응기(Applied Biosystems)에 두었다. PCR 반응 프로그램은 5분 동안 95℃의 1주기, 95℃/1분, 55℃/30초, 72℃/30초의 30주기 후, 72℃/5분의 인큐베이션이었다. 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)을 클로닝하기 위한 프라이머는 다음과 같다:

PCR 생성물의 크기를 아가로스 전기영동에 의해 검출하였다. 이후, PCR 생성물을 DNA 정제 키트(Geneaid Biotech Ltd., Taiwan)로 정제하였다. 정제 후, PCR 생성물을 pET20b 발현 벡터에 작제하였다. 정제된 PCR 생성물 및 pET20b 발현 벡터를 2개의 제한 효소 BamHI 및 HindIII로 각각 절단하였다. 제한 효소 절단 반응 후, 절단된 PCR 생성물 및 pET20b 발현 벡터를 DNA 정제 키트(Geneaid Biotech Ltd., Taiwan)로 각각 정제하였다. 정제된 PCR 생성물을 정제된 pET20b 발현 벡터와 라이게이션시켜 플라스미드 pET20b-IgG-Fc 4a를 형성시키고, 플라스미드를 숙주 세포(E. coli)에 형질전환시켰다. 형질전환주를 선택하고, 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)의 DNA 서열을 DNA 시퀀싱에 의해 확인하였다. 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)은 SEQ ID NO:3의 DNA 서열 및 SEQ ID NO:4의 아미노산 서열을 갖는다.

실시예 3 - 돼지 재조합 융합 인터페론(P IFN - Fc )을 인코딩하는 폴리뉴클레오티드 서열을 함유하는 벡터의 작제

본 실시예에서, 실시예 1에서 클로닝된 돼지 인터페론 α1(P IFNα1)의 DNA 서열(SEQ ID NO:1) 및 실시예 2에서 클로닝된 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)의 DNA 서열(SEQ ID NO:3)을 글리신 및 세린 잔기를 포함하는 링커의 DNA 서열(SEQ ID NO:5)과 연결시켜 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a)의 DNA 서열(SEQ ID NO:7)을 작제하였다.

먼저, 돼지 인터페론 α1(P IFNα1)의 DNA 서열(SEQ ID NO:1) 및 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)의 DNA 서열(SEQ ID NO:3)을 PCR에 의해 각각 증폭시켰다. 또한, 글리신 및 세린 잔기를 포함하는 링커의 DNA 서열(SEQ ID NO:5)의 5'-말단 및 3'-말단을 각각 PCR에 의한 PCR 프라이머 설계를 통해 돼지 인터페론 α1(P IFNα1)의 DNA 서열 및 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)의 DNA 서열과 함께 증폭시켰다. 돼지 인터페론 α1(P IFNα1)을 증폭시키기 위한 프라이머는 다음과 같다:

이중으로 밑줄친 뉴클레오티드는 링커의 DNA 서열의 5'-말단이다.

돼지 면역글로불린 Fc 단편(P IgG Fc)을 증폭시키기 위한 프라이머는 다음과 같다:

이중으로 밑줄친 뉴클레오티드는 링커의 DNA 서열의 3'-말단이다.

1 ㎕의 100배 희석된 pET20b-IFNα 또는 pET20b-IgG-Fc, 5 ㎕의 10x PCR 완충액, 8 ㎕의 1.25 mM dNTP, 1 ㎕의 정방향 프라이머, 1 ㎕의 역방향 프라이머, 33 ㎕의 가압증기멸균된 물, 및 1 ㎕의 Taq 중합효소를 함유하는 PCR 혼합물을 GeneAmp® PCR System 2400 반응기(Applied Biosystems)에 두었다. PCR 반응 프로그램은 5분 동안 95℃의 1주기, 95℃/1분, 55℃/30초, 72℃/30초의 30주기 후, 72℃/5분의 인큐베이션이었다.

PCR 생성물의 크기를 아가로스 전기영동에 의해 검출하였다. 이후, PCR 생성물을 DNA 정제 키트(Geneaid Biotech Ltd., Taiwan)로 정제하였다. 돼지 인터페론 α1(P IFNα1)의 정제된 PCR 생성물을 2개의 제한 효소 EcoRV 및 BamHI으로 절단하고, 돼지 면역글로불린 Fc 4a 단편(P IgG Fc 4a)의 정제된 PCR 생성물을 2개의 제한 효소 BamHI 및 HindIII로 절단하고, pET20b 발현 벡터를 2개의 제한 효소 EcoRV 및 HindIII로 절단하였다. 제한 효소 절단 반응 후, 절단된 PCR 생성물 및 pET20b 발현 벡터를 DNA 정제 키트(Geneaid Biotech Ltd., Taiwan)로 각각 정제하였다. 정제된 PCR 생성물을 정제된 pET20b 발현 벡터와 라이게이션시켜 플라스미드 pET20b-IFNα1-Fc 4a를 형성시키고, 플라스미드를 숙주 세포(E. coli)에 형질전환시켰다. 형질전환주를 선택하고, 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a)의 DNA 서열(SEQ ID NO:7)을 DNA 시퀀싱에 의해 확인하였다. 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a)는 SEQ ID NO:8의 아미노산 서열을 갖는다.

실시예 4 - 다른 돼지 재조합 융합 인터페론(P IFN - Fc )을 인코딩하는 폴리뉴클레오티드 서열을 함유하는 벡터의 작제

실시예 1에서 클로닝된 돼지 인터페론 α1(P IFNα1)의 DNA 서열(SEQ ID NO:1)에 더하여, 다른 돼지 인터페론 α 서브타입, 예를 들어, α2 내지 α17이 존재한다. 돼지 인터페론 α2 내지 α17을 인코딩하는 DNA 서열은 각각 SEQ ID NO:16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 및 46이며, 돼지 인터페론 α2 내지 α17의 아미노산 서열은 각각 SEQ ID NO:17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 및 47이다.

실시예 2에서 클로닝된 돼지 면역글로불린 Fc 4a 단편(P IgG-Fc 4a)의 DNA 서열(SEQ ID NO:3)에 더하여, 돼지 면역글로불린 Fc 1a, 1b, 2a, 2b, 및 4a 단편(P IgG-Fc 1a, 1b, 2a, 2b, 4a)을 또한 본 실시예에서 돼지 인터페론 α1 내지 α17과 각각 융합시키는데 사용하여 돼지 재조합 융합 인터페론을 작제하였다. 돼지 면역글로불린 Fc 1a, 1b, 2a, 2b, 및 4a 단편(P IgG-Fc 1a, 1b, 2a, 2b, 4a)을 인코딩하는 DNA 서열은 각각 SEQ ID NO:48, 50, 52, 54, 및 3이며, 돼지 면역글로불린 Fc 1a, 1b, 2a, 2b, 및 4a 단편의 아미노산 서열은 각각 SEQ ID NO:49, 51, 53, 55, 및 4이다.

실시예 3에 기재된 것과 같이, 돼지 인터페론 α1 내지 α17(P IFNα1-α17)을 인코딩하는 DNA 서열(SEQ ID NO:1, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 및 46) 및 돼지 면역글로불린 Fc 1a, 1b, 2a, 2b, 및 4a 단편(P IgG-Fc 1a, 1b, 2a, 2b, 4a)을 인코딩하는 DNA 서열(SEQ ID NO:48, 50, 52, 54, 및 3)을 글리신 및 세린 잔기를 포함하는 링커를 인코딩하는 DNA 서열(SEQ ID NO:5)에 의해 각각 연결시켜 다양한 돼지 재조합 융합 인터페론(P IFNα-Fc)을 인코딩하는 DNA 서열(SEQ ID NO:7, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 및 222)을 작제하였다. 상기 언급된 돼지 재조합 융합 인터페론(P IFNα-Fc)을 인코딩하는 DNA 서열을 DNA 합성기(Applied Biosystems, Inc., USA)에 의해 돼지 재조합 융합 인터페론을 인코딩하는 각각의 DNA 서열의 5'-말단에 EcoRV 절단 부위(GATATC) 및 돼지 재조합 융합 인터페론을 인코딩하는 각각의 DNA 서열의 3'-말단에 HindIII 절단 부위(AAGCTT)를 각각 갖도록 합성하였다.

5'-말단 및 3'-말단에 각각 EcoRV 절단 부위 및 HindIII 절단 부위를 갖는 돼지 재조합 융합 인터페론의 합성된 DNA 서열 각각, 및 pSecTag2(B) 발현 벡터 모두를 2개의 제한 효소 EcoRV 및 HindIII로 절단하였다. 제한 효소 절단 반응 후, 절단된 DNA 서열 및 pSecTag2(B) 발현 벡터를 DNA 정제 키트(Geneaid Biotech Ltd., Taiwan)로 각각 정제하였다. 정제된 DNA 서열을 정제된 pSecTag2(B) 발현 벡터와 라이게이션시켜 플라스미드 pSecTag2(B)-IFNα-Fc를 형성시키고, 플라스미드를 숙주 세포(E. coli)에 형질전환시켰다. 플라스미드 pSecTag2(B)-IFNα-Fc를 갖는 형질전환주를 선택하였다. 돼지 재조합 융합 인터페론(P IFNα-Fc)은 SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223의 아미노산 서열 중 하나를 갖는다.

실시예 5 - 돼지 재조합 융합 인터페론(P IFNα-Fc)의 발현

실시예 4에서 작제된 플라스미드 pSecTag2(B)-IFNα1-Fc 4a를 CHO 세포에 트랜스펙션시켰다. 먼저, 4 ㎍의 pSecTag2(B)-IFNα1-Fc 4a DNA를 15초 동안 볼텍싱(vortexing)에 의해 항생제가 없는 VP 혈청-비함유 배지(Invitrogen)에 희석시켰다(용액 A). 4 ㎍의 리포펙타민(Invitrogen)을 항생제가 없는 VP 혈청-비함유 배지(Invitrogen)에 희석시키고(용액 B), 이후 5분 동안 실온에서 인큐베이션하였다. 다음으로, 희석된 플라스미드 DNA(용액 A)를 희석된 리포펙타민(용액 B)과 혼합하고, 혼합물을 20분 동안 37℃에서 인큐베이션하였다. 이후, 혼합물(A+B)을 밤새 배양된 CHO 세포에 균일하게 첨가하고, 혼합물을 갖는 CHO 세포를 6시간 동안 5% CO₂ 인큐베이터에서 37℃에서 인큐베이션하였다. 인큐베이션 후, 혼합물을 제거하고, 10% 소 태아 혈청(FBS)을 함유하는 F12 배지를 CHO 세포에 첨가하였다. CHO 세포를 48시간 동안 5% CO₂ 인큐베이터에서 37℃에서 배양하였다.

다음으로, 돼지 재조합 융합 인터페론의 DNA 서열을 갖는 CHO 세포를 제오신을 이용하여 선택하였다. 트랜스펙션된 CHO 세포를 10% FBS, 100 Units/ml 페니실린, 100 Units/ml 스트렙토마이신, 및 700 ㎍/ml 제오신을 갖는 F12 배지 중에서 24-웰 배양 접시에서 배양하여 세포를 선택하였다. 이후, 트랜스펙션된 CHO 세포를 PBS로 2회 세척하고, 0.125% 트립신으로 해리시키고, 세포가 둥근 형태가 된 후에 접시를 진탕시켜 분리시켰다. 분리된 세포를 선택 배지에 현탁시키고, 37℃, 5% CO₂에서 배양하였다. 선택 배지를 10 내지 20%의 세포가 생존할 때까지 3 내지 4일 마다 보충하였다. 생존 세포를 세포가 거의 컨플루언스(confluence)까지 성장할 때까지 10% FBS 및 50 ㎍/ml 제오신을 갖는 F12 배지에서 배양하였다. 이후, 선택 세포로부터의 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a)의 발현을 면역형광 검정(IFA), 효소결합면역흡착측정법(ELISA), 및 적절한 항체를 이용한 웨스턴 블롯에 의해 검출하였다.

1. IFA에 의한 돼지 재조합 융합 인터페론의 생물학적 검정

샘플 세포를 24-웰 배양 플레이트에 시딩(1×10⁵ 세포/웰)하고, 80 내지 90% 컨플루언스까지 성장시켰다. 이후, 샘플 세포를 PBS로 2회 세척하고, 4℃에서 30분 동안 80% 아세톤(-20℃)으로 고정시켰다. 이후, 아세톤을 폐기하고, 세포를 PBS로 3회 세척하였다. 그 후, 세포를 어두운 곳에서 37℃에서 30분 동안 토끼 항 돼지 IgG-FITC 항체(300 ㎕/웰)(PBS 중 1:1000 희석)와 함께 인큐베이션하였다. 이후, 항혈청을 폐기하고, 세포를 PBS로 3회 세척하고, 최종적으로 형광 현미경 시험을 위해 250 ㎕ PBS에 마운팅하였다.

도 1은 형광 현미경 시험의 결과를 도시한다. 형광 신호를 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포에서 검출하였다(도 1A). 형광 신호가 pSecTag2(B)로 트랜스펙션된 후, 제오신에 의해 스크리닝된 CHO 세포에서 검출되지 않았다(도 1B).

2. ELISA에 의한 돼지 재조합 융합 인터페론의 생물학적 검정

샘플 세포를 72시간 동안 10% FBS를 갖는 F-12 배지를 함유하는 25T-플라스크에서 배양(1×10⁵ 세포/플라스크)한 후, 상층액을 수거하고, ELISA 코팅 완충액(0.1 M NaHCO₃ 및 0.1 M Na₂CO₃ pH9.6)으로 연속적으로 2배(2, 4, 8, 16, 32, 64, 및 128배) 희석시켰다. 100 ㎕의 각각의 희석된 샘플을 ELISA 플레이트(NUNC)에 첨가하고, 24시간 동안 4℃에 두었다. 그 후, 희석된 상층액을 제거하고, ELISA 플레이트를 ELISA 세척 완충액(0.9% NaCl, 0.1% Tween20)으로 3회 세척하였다. 블로킹 완충액(ELISA 세척 완충액 중 1% BSA)을 ELISA 플레이트에 첨가(100 ㎕/웰)하고, 플레이트를 1시간 동안 실온에서 인큐베이션하여, 단백질의 비-특이적 결합을 방지하였다. 이후, 블로킹 완충액을 제거하고, ELISA 플레이트를 ELISA 세척 완충액으로 3회 세척하였다. 마우스 항 IFNα 모노클로날 항체(SANTA CRUZ)를 1% BSA를 함유하는 ELISA 세척 완충액으로 500배 희석(1:500)시킨 후, ELISA 플레이트에 첨가(100 ㎕/웰)하였다. 1시간 동안 실온에서 인큐베이션시킨 후, ELISA 플레이트를 ELISA 세척 완충액으로 6회 세척하였다. 호스라디쉬 퍼옥시다제(HRP)에 컨쥬게이션된 염소 항 마우스 이차 항체(KPL)를 1% BSA를 함유하는 ELISA 세척 완충액으로 1000배 희석(1:1000)시킨 후, ELISA 플레이트에 첨가(100 ㎕/웰)하였다. 37℃에서 1시간 동안 인큐베이션시킨 후, 플레이트를 PBS로 6회 세척하였다. 결과의 시각화를 위해, 3,3',5,5'-테트라메틸벤지딘(TMB)(KPL)을 웰에 첨가하였다. 10분 동안 인큐베이션시킨 후, 신호의 흡광도를 650 nm에서 설정된 ELISA 판독기를 이용하여 판독하였다.

도 2는 ELISA 시험에 의한 돼지 재조합 융합 인터페론의 생물학적 검정의 결과를 도시한다. 돼지 재조합 융합 인터페론이 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 CHO 세포로부터의 분비물에서 검출되었다. 128배로 희석된 후에도, 재조합 융합 인터페론이 여전히 검출가능하였다. 돼지 재조합 융합 인터페론은 pSecTag2(B)로 트랜스펙션된 CHO 세포로부터의 분비물에서는 검출되지 않았다.

3. 웨스턴 블롯에 의한 돼지 재조합 융합 인터페론의 생물학적 검정

샘플 세포를 72시간 동안 10% FBS를 갖는 F-12 배지에서 배양한 후, 상층액을 수거하고, 소듐 도데실 설페이트-폴리아크릴아미드 겔 전기영동(SDS-PAGE)에 의해 분석하였다. 단백질 면역블로팅을 위해, 전기영동 후, 단백질을 PVDF 막으로 옮겼다. 생성된 막을 16 내지 24시간 동안 4℃에서 TBST(10 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.3% Tween 20) 중 5% 스킴 밀크(skim milk)를 이용하여 블로킹시켜, 단백질의 비-특이적 결합을 방지한 후, 각각 5분 동안 TBST로 3회 세척하였다. 이후, 막을 1시간 동안 실온에서 마우스 항 IFNα 모노클로날 항체(SANTA CRUZ)(0.5% 스킨 밀크(skin milk)를 함유하는 TBST에 1:500 희석)와 함께 인큐베이션하였다. 이후, 블롯을 TBST로 6회 세척하고, 1시간 동안 실온에서 알칼리성 포스파타제(AP) 컨쥬게이션된 염소 항-마우스 IgG 모노클로날 항체(0.5% 스킨 밀크를 함유하는 TBST에 1:2000 희석)와 함께 인큐베이션시켰다. 이후, 블롯을 TBST로 6회 세척하였다. 밴드를 5분 동안 니트로 블루 테트라졸륨(NBT)/5-브로모-4-클로로-3-인돌릴-포스페이트(BCIP) 기질을 이용하여 검출한 후, 물로 세척하여 반응을 정지시켰다. 또한, 돼지 재조합 융합 인터페론을 알칼리성 포스파타제(AP) 컨쥬게이션된 염소 항-돼지 IgG 항체(KPL) 및 알칼리성 포스파타제(AP) 컨쥬게이션된 마우스 항 6×His 모노클로날 항체(invitrogen)를 이용하여 또한 검출하였다.

도 3은 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 CHO 세포에서 발현된 단백질의 웨스턴 블롯의 결과를 도시한다. 돼지 재조합 융합 인터페론을 마우스 항 IFNα 모노클로날 항체(레인 2), 마우스 항 6×His 모노클로날 항체(레인 3), 및 염소 항-돼지 IgG 항체(레인 4)에 의해 검출하였다. 결과는 돼지 재조합 융합 인터페론을 인코딩하는 DNA 서열을 함유하는 플라스미드로 트랜스펙션된 CHO 세포가 돼지 재조합 융합 인터페론을 분비하는 것을 나타낸다.

실시예 6 - 돼지 재조합 융합 인터페론(P IFN α- Fc )의 소규모 및 대규모 생성 및 돼지 재조합 융합 인터페론의 PRRSV 에 대한 항바이러스 활성의 평가

1. 돼지 재조합 융합 인터페론(P IFN α- Fc )의 소규모 생성

돼지 재조합 융합 인터페론(P INFα1-Fc 4a)의 DNA 서열(SEQ ID NO:7)을 포함하는 CHO 세포를 25 cm² 세포 배양 플라스크에 2×10⁶ 세포의 밀도로 시딩하고, 24시간 동안 10% FBS, 100 Units/ml 페니실린, 및 100 Units/ml 스트렙토마이신을 갖는 F12 배지에서 배양하였다. 이후, 배지를 제거하였다. 세포를 PBS로 세척한 후, 100 Units/ml 페니실린 및 100 Units/ml 스트렙토마이신을 갖는 CHO-S-SFM II 혈청-비함유 배지(GIBCO)에서 배양하였다. 상층액을 수거하고, 100 Units/ml 페니실린 및 100 Units/ml 스트렙토마이신을 함유하는 새로운 혈청-비함유 배지를 24, 48, 및 72시간 마다 각각 첨가하였다. 돼지 재조합 융합 인터페론을 함유하는 상층액을 10분 동안 원심분리(1,000 rpm)시켜, 세포 및 세포 부스러기를 제거하였다. 이후, 수거된 돼지 재조합 융합 인터페론의 항바이러스 활성을 돼지 생식기 호흡기 증후군 바이러스(PRRS)로 평가하였다.

2. 돼지 재조합 융합 인터페론의 PRRSV 에 대한 항바이러스 활성의 평가

수거된 돼지 재조합 융합 인터페론을 1% FBS, 100 Units/ml 페니실린, 및 100 Units/ml 스트렙토마이신을 함유하는 MEM 배지로 연속 희석(10, 20, 40, 80, 160, 320, 640, 1280, 및 2560배)시켰다. MARC-145 세포를 96-웰 세포 배양 플레이트에서 1.5×10⁴ 세포/웰의 밀도로 시딩하고, 16 내지 24시간 동안 37℃, 5% CO₂에서 배양하였다. 배양 배지를 제거한 후, 세포를 희석된 돼지 재조합 융합 인터페론(100 ㎕/웰, n = 4)으로 처리한 후, 24시간 동안 37℃, 5% CO₂에서 배양하였다. 희석된 샘플을 제거한 후, 세포를 PRRS 바이러스(100 TCID₅₀/100㎕)로 감염시킨 후, 세포의 90%가 세포병변 효과(CPE)를 나타낼 때까지 약 120시간 동안 37℃, 5% CO₂에서 배양하였다. 돼지 재조합 융합 인터페론으로 처리된 후, PRRS 바이러스로 감염된 세포에 더하여, 인터페론으로 처리되지 않고 PRRS 바이러스로 감염되지 않은 세포, PRRS 바이러스로 감염되었으나 인터페론으로 처리되지 않은 세포, 및 인터페론으로 처리되었으나 PRRS 바이러스로 감염되지 않은 세포의 3개의 다른 그룹이 존재하였다. 이후, 세포를 사용하여 돼지 재조합 융합 인터페론(P IFN-Fc)의 항바이러스 활성을 평가하였다. 세포 현탁액을 분리시키고, 세포를 PBS로 2회 세척한 후, 30분 동안 4℃에서 80% 아세톤(-20℃, 100 ㎕/웰)을 갖는 플레이트에 고정시켰다. 아세톤을 제거한 후, 세포를 PBS로 3회 세척하고, 20분 동안 1% 메틸로사닐린 클로라이드로 염색하였다. 그 후, 세포를 증류수로 5회 세척한 후, 100% 에탄올을 첨가하여 메틸로사닐린 클로라이드를 용해시켰다. 10분 후, 신호의 흡광도를 550 nm에서 설정된 ELISA 판독기를 이용하여 판독하였다. 돼지 재조합 융합 인터페론(P IFN-Fc)의 농도를 하기 식에 의해 계산하였다.

상기 식에서, OD maximum은 IFN으로 처리되거나 처리되지 않은 감염되지 않은 세포 단층의 흡광도(보호 100%)이고, OD minimum은 감염된 보호되지 않은 세포 단층의 흡광도(보호 0)이다.

상기 식에서, T_n은 OD₅₀ _% 바로 위의 OD에 해당하는 IFN 희석의 역수이고, T_n ₊₁은 OD₅₀ _% 바로 아래의 OD에 해당하는 IFN 희석의 역수이고, OD_n은 OD₅₀ _% 바로 위의 흡광도 값이고, OD_n ₊₁은 OD₅₀ _% 바로 아래의 흡광도 값이다.

표 1은 상기 기재된 소규모 생성 방법에 의해 생성된 돼지 재조합 융합 인터페론의 PRRSV에 대한 항바이러스 활성을 나타낸다. 결과는 돼지 재조합 융합 인터페론이 PRRSV에 대한 항바이러스 활성을 갖는 것을 나타낸다.

표 1 - 25 cm² 세포 배양 플라스크에서 생성된 돼지 재조합 융합 인터페론의 PRRSV에 대한 항바이러스 활성(IU/ml).

표 2는 돼지 재조합 융합 인터페론의 농도에 25 cm² 세포 배양 플라스크의 부피(5 ml)를 곱하여 계산된 소규모 생성 방법에 의해 생성된 돼지 재조합 융합 인터페론의 양을 나타낸다.

표 2 - 25 cm² 세포 배양 플라스크(25T-플라스크)에서 생성된 돼지 재조합 융합 인터페론의 양(단위: IU/5 ml).

3. 돼지 재조합 융합 인터페론(P IFN α- Fc )의 대규모 생성

돼지 재조합 융합 인터페론(P INFα1-Fc 4a)의 DNA 서열(SEQ ID NO:7)을 포함하는 CHO 세포를 먼저 175 cm² 세포 배양 플라스크에서 배양하였다. 세포의 단층이 형성된 후, 세포를 0.125% 트립신으로 해리시키고, 10% FBS, 100 Units/ml 페니실린, 및 세포 계수를 위한 100 Units/ml 스트렙토마이신을 함유하는 F12 배지에 현탁시켰다. 이후, 세포를 각각 6.8×10⁷ 세포/병 및 8×10⁷ 세포/병의 농도로 회전 병에 시딩하고, 37℃, 0.167 rpm에서 10% FBS를 함유하는 200 ml의 F12 배지에서 배양하였다. 24시간 후, 세포를 PBS로 세척하고, 100 Units/ml 페니실린 및 100 Units/ml 스트렙토마이신을 함유하는 CHO-S-SFM II 혈청-비함유 배지(Invitrogen)에서 배양하였다. 상층액을 수거하고, 100 units/ml 페니실린 및 100 units/ml 스트렙토마이신을 함유하는 새로운 혈청-비함유 배지를 72시간 마다 6회 첨가하였다. 돼지 재조합 융합 인터페론을 포함하는 상층액을 10분 동안 원심분리(1,000 rpm)하여 세포 및 세포 부스러기를 제거하였다. 돼지 재조합 융합 인터페론의 농도를 상기 기재된 MTT 방법을 이용하여 PRRSV에 대한 이의 항바이러스 활성을 평가함으로써 계산하였다.

표 3은 회전 병에 의해 생성된 돼지 재조합 융합 인터페론의 농도를 나타낸다. 결과는 회전 병에 의해 생성된 돼지 재조합 융합 인터페론이 25 cm² 세포 배양 플라스크에 의해 생성된 재조합 융합 인터페론보다 PRRSV에 대해 더 높은 항바이러스 활성을 갖는 것을 나타낸다.

표 3 - 회전 병에서 생성된 돼지 재조합 융합 인터페론(P IFN-Fc)의 농도(IU/ml).

또한, 표 4는 대규모 생성 방법에 의해 생성된 돼지 재조합 융합 인터페론의 항바이러스 활성(PRRS 바이러스에 대한 활성)의 전체량을 나타낸다.

표 4 - 회전 병에서 생성된 돼지 재조합 융합 인터페론의 항바이러스 활성의 전체량(단위: IU/200 ml).

실시예 7 - 돼지 재조합 융합 인터페론(P IFN α- Fc )의 PRRSV 에 대한 항바이러스 활성 및 돼지 면역글로불린 Fc 단편( IgG - Fc )이 없는 돼지 인터페론(P IFN α)의 PRRSV 에 대한 항바이러스 활성의 비교

MARC-145 세포를 16 내지 24시간 동안 37℃, 5% CO₂에서 96-웰 세포 배양 플레이트에서 1.5×10⁴ 세포/웰의 밀도로 배양하였다. 배양 배지를 제거한 후, 세포를 16 내지 24시간 동안 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 또는 돼지 면역글로불린 Fc 단편이 없는 돼지 인터페론(P IFNα1 그룹)(SEQ ID NO:2)으로 처리하였다. 2개 유형의 인터페론을 제거한 후, 세포를 PRRS 바이러스(100 TCID₅₀/ 100㎕)로 감염시킨 후, 4 내지 5일 동안 37℃, 5% CO₂에서 배양하였다. 이후, 세포 생활력을 MTT 방법에 의해 분석하였다.

표 5 및 도 4는 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 및 돼지 인터페론(P IFNα1 그룹)의 PRRSV에 대한 항바이러스 활성의 검정 결과를 나타낸다. 결과는 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹)이 돼지 인터페론(P IFNα1 그룹)보다 PRRSV에 대해 더 높은 항바이러스 활성을 갖는 것을 나타낸다.

표 5 - 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 및 돼지 인터페론(P IFNα1 그룹)의 PRRSV에 대한 항바이러스 활성의 비교.

실시예 8 - 돼지 재조합 융합 인터페론(P IFN -α Fc ) 및 돼지 면역글로불린 Fc 단편( IgG - Fc )이 없는 돼지 인터페론(P IFN α)의 PRV 에 대한 항바이러스 활성의 비교

ST 세포를 16 내지 24시간 동안 37℃, 5% CO₂에서 96-웰 세포 배양 플레이트에서 1.5×10⁴ 세포/웰의 밀도로 배양하였다. 배양 배지를 제거한 후, 세포를 16 내지 24시간 동안 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 또는 돼지 면역글로불린 Fc 단편을 갖지 않는 돼지 인터페론(P IFNα1 그룹)(SEQ ID NO:2)으로 처리하였다. 2개 유형의 인터페론을 제거한 후, 세포를 PR 바이러스(1 TCID₅₀/ 100㎕)로 감염시킨 후, 4 내지 5일 동안 37℃, 5% CO₂에서 배양하였다. 이후, 세포 생활력을 MTT 방법에 의해 분석하였다.

표 6 및 도 4는 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 및 돼지 인터페론(P IFNα1 그룹)의 PRV에 대한 항바이러스 활성의 검정 결과를 나타낸다. 결과는 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹)이 돼지 인터페론(P IFNα1 그룹)보다 PRV에 대해 더 높은 항바이러스 활성을 갖는 것을 나타낸다.

표 6 - 돼지 재조합 융합 인터페론(P IFNα1-Fc 4a 그룹) 및 돼지 인터페론(P IFNα1 그룹)의 PRV에 대한 항바이러스 활성의 비교.

유사하게, 실시예 4에서 작제된 다른 돼지 재조합 융합 인터페론의 DNA 서열(SEQ ID NO:7, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 및 222)을 함유하는 플라스미드 pSecTag2(B)-IFNα-Fc를 실시예 5에 기재된 방법에 의해 CHO 세포에 트랜스펙션시켜, 돼지 재조합 융합 인터페론(P IFNα-Fc)(SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223)을 발현시켰다. 발현된 돼지 재조합 융합 인터페론(P IFNα-Fc)의 PRRS 바이러스 및 PR 바이러스에 대한 항바이러스 활성을 실시예 7 및 8에 기재된 방법에 의해 평가하였다. 결과는 돼지 재조합 융합 인터페론(P IFNα-Fc)이 돼지 면역글로불린 Fc 단편이 없는 돼지 인터페론보다 높은 항바이러스 활성을 갖는 것을 나타낸다.

상기 실시예의 결과를 기초로 하여, 본 발명의 동물용 재조합 융합 인터페론은 돼지 면역글로불린 Fc 단편이 없는 동물 인터페론보다 RNA 바이러스 및 DNA 바이러스 둘 모두에 대해 더 높은 항바이러스 활성을 갖는다.

본 발명의 상기 기재된 구현예에서 많은 변화 및 변형이 물론 본 발명의 범위로부터 벗어남이 없이 수행될 수 있다. 따라서, 과학 및 유용한 분야에서 발달을 촉진시키기 위해, 본 발명이 개시되며, 이는 첨부된 청구항의 범위에 의해서만 제한된다.

SEQUENCE LISTING <110> SBC Virbac Biotech Co., Ltd. <120> Interferon And Immune Globulin Fc Section Fusion Protein <130> P14-0127 <150> CN201210016618.8 <151> 2012-01-19 <150> PCT/CN2013/070693 <151> 2013-01-18 <160> 223 <170> PatentIn version 3.5 <210> 1 <211> 543 <212> DNA <213> Sus scrofa <400> 1 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacaaa tgaggagaat ctcccccttc tcctgcctgg accacagaag ggactttgga 180 ttcccccaag aggccttggg gggcaaccag gtccagaagg ctcaagccat ggctctggtg 240 catgagatgc tccagcagac cttccagctc ttcagcacag agggctcggc tgctgcctgg 300 gatgagagcc tcctgcacca gttctgcact ggactggatc agcagctcag ggacctggaa 360 gcctgtgtca tgcaggaggc ggggctggaa gggacccccc tgctggagga ggactccatc 420 ctggctgtga ggaaatactt ccacagactc accctctatc tgcaagagaa gagctacagc 480 ccctgtgcct gggagatcgt cagagcagaa gtcatgagag ccttctcttc ctccagaaac 540 ctg 543 <210> 2 <211> 181 <212> PRT <213> Sus scrofa <400> 2 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly 115 120 125 Leu Glu Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg 130 135 140 Lys Tyr Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser 145 150 155 160 Pro Cys Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser 165 170 175 Ser Ser Arg Asn Leu 180 <210> 3 <211> 690 <212> DNA <213> Sus scrofa <400> 3 ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc cgggccctcg 60 gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac ccccaaggtc 120 acgtgcgtgg tggtagatgt gagccaggag aacccggagg tccagttctc ctggtacgtg 180 gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt caacagcacc 240 taccgcgtgg tcagcgtcct gcccatccag caccaggact ggctgaacgg gaaggagttc 300 aagtgcaagg tcaacaacaa agacctccca gcccccatca caaggatcat ctccaaggcc 360 aaagggcaga cccgggagcc gcaggtgtac accctgcccc cacccaccga ggagctgtcc 420 aggagcaaag tcacgctaac ctgcctggtc actggcttct acccacctga catcgatgtc 480 gagtggcaaa gaaacggaca gccggagcca gagggcaatt accgcaccac cccgccccag 540 caggacgtgg acgggaccta cttcctgtac agcaagctcg cggtggacaa ggccagctgg 600 cagcgtggag acacattcca gtgtgcggtg atgcacgagg ctctgcacaa ccactacacc 660 cagaagtcca tcttcaagac tccgggtaaa 690 <210> 4 <211> 230 <212> PRT <213> Sus scrofa <400> 4 Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly 1 5 10 15 Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 20 25 30 Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val Asp Val Ser 35 40 45 Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu 50 55 60 Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr 65 70 75 80 Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn 85 90 95 Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro 100 105 110 Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln 115 120 125 Val Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys Val 130 135 140 Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp Val 145 150 155 160 Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr 165 170 175 Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys 180 185 190 Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln Cys 195 200 205 Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile 210 215 220 Phe Lys Thr Pro Gly Lys 225 230 <210> 5 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of a linker of interferon and IgG Fc fragment <400> 5 ggtggctcag gtggatccgg tggaggcgga agcggcggtg gaggatca 48 <210> 6 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of a linker of interferon and IgG Fc fragment <400> 6 Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 15 <210> 7 <211> 1281 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa1+IgG-Fc 4a <400> 7 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacaaa tgaggagaat ctcccccttc tcctgcctgg accacagaag ggactttgga 180 ttcccccaag aggccttggg gggcaaccag gtccagaagg ctcaagccat ggctctggtg 240 catgagatgc tccagcagac cttccagctc ttcagcacag agggctcggc tgctgcctgg 300 gatgagagcc tcctgcacca gttctgcact ggactggatc agcagctcag ggacctggaa 360 gcctgtgtca tgcaggaggc ggggctggaa gggacccccc tgctggagga ggactccatc 420 ctggctgtga ggaaatactt ccacagactc accctctatc tgcaagagaa gagctacagc 480 ccctgtgcct gggagatcgt cagagcagaa gtcatgagag ccttctcttc ctccagaaac 540 ctgggtggct caggtggatc cggtggaggc ggaagcggcg gtggaggatc aggaacaaag 600 accaaaccac catgtcccat atgcccagcc tgtgaagggc ccgggccctc ggccttcatc 660 ttccctccaa aacccaagga caccctcatg atctcccgga cccccaaggt cacgtgcgtg 720 gtggtagatg tgagccagga gaacccggag gtccagttct cctggtacgt ggacggcgta 780 gaggtgcaca cggcccagac gaggccaaag gaggagcagt tcaacagcac ctaccgcgtg 840 gtcagcgtcc tgcccatcca gcaccaggac tggctgaacg ggaaggagtt caagtgcaag 900 gtcaacaaca aagacctccc agcccccatc acaaggatca tctccaaggc caaagggcag 960 acccgggagc cgcaggtgta caccctgccc ccacccaccg aggagctgtc caggagcaaa 1020 gtcacgctaa cctgcctggt cactggcttc tacccacctg acatcgatgt cgagtggcaa 1080 agaaacggac agccggagcc agagggcaat taccgcacca ccccgcccca gcaggacgtg 1140 gacgggacct acttcctgta cagcaagctc gcggtggaca aggccagctg gcagcgtgga 1200 gacacattcc agtgtgcggt gatgcacgag gctctgcaca accactacac ccagaagtcc 1260 atcttcaaga ctccgggtaa a 1281 <210> 8 <211> 427 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa1+IgG-Fc 4a <400> 8 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly 115 120 125 Leu Glu Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg 130 135 140 Lys Tyr Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser 145 150 155 160 Pro Cys Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser 165 170 175 Ser Ser Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys 195 200 205 Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys 210 215 220 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val 225 230 235 240 Val Val Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr 245 250 255 Val Asp Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu 260 265 270 Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His 275 280 285 Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys 290 295 300 Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln 305 310 315 320 Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu 325 330 335 Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro 340 345 350 Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu 355 360 365 Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr 370 375 380 Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly 385 390 395 400 Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu His Asn His Tyr 405 410 415 Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 <210> 9 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for interferon <400> 9 cccaagctta tggccccaac ctcagcc 27 <210> 10 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for interferon <400> 10 ccgctcgagc aggtttctgg aggaaga 27 <210> 11 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for IgG Fc fragment <400> 11 cgggatccgg gaacaaagac c 21 <210> 12 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for IgG Fc fragment <400> 12 cccaagcttt ttacccggag tc 22 <210> 13 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for interferon <400> 13 gcgatatcat ggccccaacc tc 22 <210> 14 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for interferon <400> 14 cgggatccac ctgagccacc caggtttctg gagg 34 <210> 15 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for IgG Fc fragment <400> 15 cgggatccgg tggaggcgga agcggcggtg gaggatcagg aacaaaga 48 <210> 16 <211> 538 <212> DNA <213> Sus scrofa <400> 16 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtga 538 <210> 17 <211> 179 <212> PRT <213> Sus scrofa <400> 17 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu <210> 18 <211> 538 <212> DNA <213> Sus scrofa <400> 18 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtga 538 <210> 19 <211> 179 <212> PRT <213> Sus scrofa <400> 19 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu <210> 20 <211> 562 <212> DNA <213> Sus scrofa <400> 20 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt ga 562 <210> 21 <211> 187 <212> PRT <213> Sus scrofa <400> 21 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu 180 185 <210> 22 <211> 562 <212> DNA <213> Sus scrofa <400> 22 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt ga 562 <210> 23 <211> 187 <212> PRT <213> Sus scrofa <400> 23 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu 180 185 <210> 24 <211> 562 <212> DNA <213> Sus scrofa <400> 24 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt ga 562 <210> 25 <211> 187 <212> PRT <213> Sus scrofa <400> 25 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu 180 185 <210> 26 <211> 538 <212> DNA <213> Sus scrofa <400> 26 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaa 538 <210> 27 <211> 179 <212> PRT <213> Sus scrofa <400> 27 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu <210> 28 <211> 564 <212> DNA <213> Sus scrofa <400> 28 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtga 564 <210> 29 <211> 187 <212> PRT <213> Sus scrofa <400> 29 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu 180 185 <210> 30 <211> 562 <212> DNA <213> Sus scrofa <400> 30 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt ga 562 <210> 31 <211> 187 <212> PRT <213> Sus scrofa <400> 31 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu 180 185 <210> 32 <211> 538 <212> DNA <213> Sus scrofa <400> 32 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtga 538 <210> 33 <211> 179 <212> PRT <213> Sus scrofa <400> 33 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu <210> 34 <211> 538 <212> DNA <213> Sus scrofa <400> 34 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaa 538 <210> 35 <211> 179 <212> PRT <213> Sus scrofa <400> 35 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu <210> 36 <211> 564 <212> DNA <213> Sus scrofa <400> 36 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtga 564 <210> 37 <211> 187 <212> PRT <213> Sus scrofa <400> 37 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu 180 185 <210> 38 <211> 562 <212> DNA <213> Sus scrofa <400> 38 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt ga 562 <210> 39 <211> 187 <212> PRT <213> Sus scrofa <400> 39 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu 180 185 <210> 40 <211> 562 <212> DNA <213> Sus scrofa <400> 40 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt ga 562 <210> 41 <211> 187 <212> PRT <213> Sus scrofa <400> 41 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu 180 185 <210> 42 <211> 559 <212> DNA <213> Sus scrofa <400> 42 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtga 559 <210> 43 <211> 186 <212> PRT <213> Sus scrofa <400> 43 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu 180 185 <210> 44 <211> 562 <212> DNA <213> Sus scrofa <400> 44 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt ga 562 <210> 45 <211> 187 <212> PRT <213> Sus scrofa <400> 45 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu 180 185 <210> 46 <211> 538 <212> DNA <213> Sus scrofa <400> 46 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtga 538 <210> 47 <211> 179 <212> PRT <213> Sus scrofa <400> 47 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu <210> 48 <211> 680 <212> DNA <213> Sus scrofa <400> 48 ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc cgggccctcg 60 gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac ccccgaggtc 120 acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct ggtacgtgga 180 cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca acagcaccta 240 ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag gagttcaagt 300 gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc aaggctatag 360 ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct gtccaggagc 420 aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca tgttgagtgg 480 aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc agcaggacgt 540 ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat gggaccatgg 600 agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc cagaagtcca 660 tctccaagac tcagggtaaa 680 <210> 49 <211> 228 <212> PRT <213> Sus scrofa <400> 49 Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val 1 5 10 15 Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 20 25 30 Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 35 40 45 Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu 50 55 60 Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr 65 70 75 80 Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys 85 90 95 Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro 100 105 110 Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr 115 120 125 Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val 130 135 140 Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp 145 150 155 160 Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro 165 170 175 Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala 180 185 190 Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val 195 200 205 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys 210 215 220 Thr Gln Gly Lys 225 <210> 50 <211> 685 <212> DNA <213> Sus scrofa <400> 50 ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc cgggccctcg 60 gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac ccccgaggtc 120 acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc tggtacgtgg 180 acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc aacagcacct 240 accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga aggagttcaa 300 gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct ccaaggctat 360 agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga gctgtccagg 420 agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat ccatgttgag 480 tggaagagca acggacagcc ggagccagga acacataccg caccaccccg ccccagcagg 540 acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca agatgggacc 600 atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact acacccagaa 660 gtccatctcc aagactcagg gtaaa 685 <210> 51 <211> 228 <212> PRT <213> Sus scrofa <400> 51 Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val 1 5 10 15 Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 20 25 30 Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 35 40 45 Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu 50 55 60 Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr 65 70 75 80 Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys 85 90 95 Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro 100 105 110 Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr 115 120 125 Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu 130 135 140 Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp 145 150 155 160 Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro 165 170 175 Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala 180 185 190 Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val 195 200 205 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys 210 215 220 Thr Gln Gly Lys 225 <210> 52 <211> 680 <212> DNA <213> Sus scrofa <400> 52 ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc agggccctcg 60 gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac accccaggtc 120 acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct ggtacgtgga 180 cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca acagcaccta 240 ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag gagttcaagt 300 gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc aaggccaaag 360 ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct gtccaggagc 420 aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga tgtcgagtgg 480 caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc agcaggacgt 540 ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct ggcagggtgg 600 aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc cagaagtcta 660 tctccaagac tccgggtaaa 680 <210> 53 <211> 228 <212> PRT <213> Sus scrofa <400> 53 Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser 1 5 10 15 Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 20 25 30 Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val Asp Val Ser 35 40 45 Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu 50 55 60 Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr 65 70 75 80 Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn 85 90 95 Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro 100 105 110 Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr 115 120 125 Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile 130 135 140 Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp 145 150 155 160 Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro 165 170 175 Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser 180 185 190 Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val 195 200 205 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys 210 215 220 Thr Pro Gly Lys 225 <210> 54 <211> 680 <212> DNA <213> Sus scrofa <400> 54 ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc agggccctcg 60 gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac accccaggtc 120 acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct ggtacgtgga 180 cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca acagcaccta 240 ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag gagttcaagt 300 gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc aaggccaaag 360 ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct gtccaggagc 420 aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga tgtcgagtgg 480 caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc agcaggacgt 540 ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct ggcagggtgg 600 aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc cagaagtcta 660 tctccaagac tccgggtaaa 680 <210> 55 <211> 228 <212> PRT <213> Sus scrofa <400> 55 Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser 1 5 10 15 Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 20 25 30 Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val Asp Val Ser 35 40 45 Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu 50 55 60 Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr 65 70 75 80 Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn 85 90 95 Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro 100 105 110 Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr 115 120 125 Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile 130 135 140 Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp 145 150 155 160 Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro 165 170 175 Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser 180 185 190 Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val 195 200 205 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys 210 215 220 Thr Pro Gly Lys 225 <210> 56 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa1+IgG-Fc 1a <400> 56 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 57 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa1+IgG-Fc 1a <400> 57 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 58 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa1+IgG-Fc 1b <400> 58 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 59 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa1+IgG-Fc 1b <400> 59 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 60 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa1+IgG-Fc2a <400> 60 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 61 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa1+IgG-Fc 2a <400> 61 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 62 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa1+IgG-Fc 2b <400> 62 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 63 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa1+IgG-Fc 2b <400> 63 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 64 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa2+IgG-Fc1a <400> 64 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 65 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa2+IgG-Fc 1a <400> 65 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 66 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa2+IgG-Fc 1b <400> 66 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 67 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa2+IgG-Fc 1b <400> 67 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 68 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa2+IgG-Fc 2a <400> 68 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 69 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa2-IgG-Fc2a <400> 69 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 70 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa2+IgG-Fc2b <400> 70 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 71 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa2+IgG-Fc 2b <400> 71 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 72 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa2+IgG-Fc 4a <400> 72 atggccccaa cctcagcctt cctcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 73 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa2+IgG-Fc 4a <400> 73 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 <210> 74 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa3+IgG-Fc1a <400> 74 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 75 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa3+IgG-Fc 1a <400> 75 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 76 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa3+IgG-Fc 1b <400> 76 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 77 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa3+IgG-Fc 1b <400> 77 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 78 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa3+IgG-Fc 2a <400> 78 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 79 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa3+IgG-Fc 2a <400> 79 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 80 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa3+IgG-Fc 2b <400> 80 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 81 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa3+IgG-Fc 2b <400> 81 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 82 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa3+IgG-Fc 4a <400> 82 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccacaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 83 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa3+IgG-Fc 4a <400> 83 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 <210> 84 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa4+IgG-Fc 1a <400> 84 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 85 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa4+IgG-Fc 1a <400> 85 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 86 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa4+IgG-Fc 1b <400> 86 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 87 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa4+IgG-Fc 1b <400> 87 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 88 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa4+IgG-Fc 2a <400> 88 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 89 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa4+IgG-Fc 2a <400> 89 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 90 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa4+IgG-Fc 2b <400> 90 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 91 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa4+IgG-Fc 2b <400> 91 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 92 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa4+IgG-Fc 4a <400> 92 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagattcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 93 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa4+IgG-Fc 4a <400> 93 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Phe Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 94 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa5+IgG-Fc 1a <400> 94 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 95 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa5+IgG-Fc 1a <400> 95 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 96 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa5+IgG-Fc 1b <400> 96 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 97 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa5+IgG-Fc 1b <400> 97 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 98 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa5+IgG-Fc 2a <400> 98 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 99 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa5+IgG-Fc 2a <400> 99 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 100 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INF a5+IgG-Fc 2b <400> 100 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 101 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa5+IgG-Fc 2b <400> 101 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 102 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa5+IgG-Fc 4a <400> 102 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctac 60 tctctgggct gtgacctgcc tcagacctac agcctggctc acaccagggc cctgaggccc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac tacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaacctcc cagctcttca gcacagaggg ctcagctgct gcctgggatg 300 acagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 103 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa5+IgG-Fc 4a <400> 103 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Tyr Ser Leu Gly Cys Asp Leu Pro Gln Thr Tyr Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Pro Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp Tyr Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Ser Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Asp Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 104 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa6+IgG-Fc 1a <400> 104 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 105 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa6+IgG-Fc 1a <400> 105 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 106 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa6+IgG-Fc 1b <400> 106 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 107 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa6+IgG-Fc 1b <400> 107 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 108 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa6+IgG-Fc 2a <400> 108 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 109 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa6+IgG-Fc 2a <400> 109 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 110 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa6+IgG-Fc 2b <400> 110 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 111 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa6+IgG-Fc 2b <400> 111 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 112 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa6+IgG-Fc 4a <400> 112 atggccccaa cctcagcctt cctcacggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctacc tcagacacac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatat ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 113 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa6+IgG-Fc 4a <400> 113 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 114 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa7+IgG-Fc 1a <400> 114 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 115 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa7+IgG-Fc 1a <400> 115 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 116 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa7+IgG-Fc 1b <400> 116 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 117 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa7+IgG-Fc 1b <400> 117 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 118 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa7+IgG-Fc 2a <400> 118 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 119 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa7+IgG-Fc 2a <400> 119 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 120 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa7+IgG-Fc 2b <400> 120 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 121 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa7+IgG-Fc2b <400> 121 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 122 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa7+IgG-Fc 4a <400> 122 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 123 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa7+IgG-Fc4a <400> 123 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 <210> 124 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa8+IgG-Fc1a <400> 124 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagg ctgtgaagtg 660 gccgggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccag 720 acccccgagg tcacgtgcgg tggtggacgt cagcaaggag cacgccgagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggccgagacg agaccaaagg aggagcagtt 840 caacagcacc taccgtgtgg tcagctctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agttacaccc tgcccccacc cgccgaggag 1020 ctgtccagga gcaaagtcac cgtaacctgc ctggtcattg gcttctaccc acctgacatc 1080 catgttgagt ggaagagcaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga ccttcttcct gtacagcaag ctcgcggtgg acaaggcaag 1200 atgggaccat ggagaaacat ttgagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc catctccaag actcagggta aa 1292 <210> 125 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa8+IgG-Fc1a <400> 125 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 126 <211> 1297 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa8+IgG-Fc 1b <400> 126 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaataca ccagccgcaa acatgtccca tatgcccagg ctgtgaagtg 660 gccgggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccag 720 acccccgagg tcacgtgcgg gtggtggacg tcagcaagga gcacgccgag gtccagttct 780 cctggtacgt ggacggcgta gaggtgcaca cggccgagac gagaccaaag gaggagcagt 840 tcaacagcac ctaccgtgtg gtcagctcct gcccatccag caccaggact ggctgaaggg 900 gaaggagttc aagtgcaagg tcaacaacgt agacctccca gcccccatca cgaggaccat 960 ctccaaggct atagggcaga gccgggagcc gcagtgtaca ccctgccccc acccgccgag 1020 gagctgtcca ggagcaaagt cacgctaacc tgcctggtca ttggcttcta cccacctgac 1080 atccatgttg agtggaagag caacggacag ccggagccag gaacacatac cgcaccaccc 1140 cgccccagca ggacgtggac gggaccttct tcctgtacag caaactcgcg gtggacaagg 1200 caagatggga ccatggagac aaatttgagt gtgcggtgat gcacgagctc tgcacaacca 1260 ctacacccag aagtccatct ccaagactca gggtaaa 1297 <210> 127 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa8+IgG-Fc1b <400> 127 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 128 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa8+IgG-Fc2a <400> 128 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaatca 660 ccagggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acaccccagg tcacgtgcgg tggttgatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctac ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccaca cgccgaggag 1020 ctgtccagga gcaaagtcag cataacctgc ctggtcattg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ttctcggtgg acaaggccag 1200 ctggcagggt ggaggcatat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc tatctccaag actccgggta aa 1292 <210> 129 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa8+IgG-Fc2a <400> 129 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 130 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa8+IgG-Fc2b <400> 130 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaatcg 660 ccagggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acaccccagg tcacgtgcgg tagttgatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctgc ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccaca cgccgaggag 1020 ctgtccagga gcaaagtcag cataacctgc ctggtcattg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ttctcggtgg acaaggccag 1200 ctggcagggt ggaggcatat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc tatctccaag actccgggta aa 1292 <210> 131 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa8+IgG-Fc2b <400> 131 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 132 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa8+IgG-Fc4a <400> 132 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggccggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagccttct cttcctccac aaacctgcaa 540 gacagactca ggaggaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaaggg 660 cccgggccct cggccttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acccccaagg tcacgtgcgg tggtagatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctgc ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccacc caccgaggag 1020 ctgtccagga gcaaagtcac gctaacctgc ctggtcactg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ctcgcggtgg acaaggccag 1200 ctggcagcgt ggagacacat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc catcttcaag actccgggta aa 1292 <210> 133 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa8+IgG-Fc4a <400> 133 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 134 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa9+IgG-Fc 1a <400> 134 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 135 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa9+IgG-Fc1a <400> 135 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 136 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa9+IgG-Fc1b <400> 136 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 137 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa9+IgG-Fc1b <400> 137 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 138 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa9+IgG-Fc2a <400> 138 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 139 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa9+IgG-Fc2a <400> 139 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 140 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa9+IgG-Fc2b <400> 140 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 141 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa9+IgG-Fc2b <400> 141 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 142 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa9+IgG-Fc4a <400> 142 atggccccaa cctcagcctt cctcatggcc ctggtgctgc tcagctgcaa ggccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttgggtt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 143 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa9+IgG-Fc4a <400> 143 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Lys Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 144 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa10+IgG-Fc1a <400> 144 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 145 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa10+IgG-Fc1a <400> 145 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 146 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa10+IgG-Fc1b <400> 146 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 147 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa10+IgG-Fc1b <400> 147 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 148 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa10+IgG-Fc2a <400> 148 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 149 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa10+IgG-Fc2a <400> 149 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 150 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa10+IgG-Fc2b <400> 150 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 151 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa10+IgG-Fc2b <400> 151 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 152 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa10+IgG-Fc4a <400> 152 atggccccaa cctcagcctt cctcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaagg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag atccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 153 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa10+IgG-Fc4a <400> 153 Met Ala Pro Thr Ser Ala Phe Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Lys Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ser Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 <210> 154 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa11+IgG-Fc1a <400> 154 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 155 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa11+IgG-Fc1a <400> 155 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 156 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa11+IgG-Fc1b <400> 156 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 157 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa11+IgG-Fc1b <400> 157 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 158 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa11+IgG-Fc2a <400> 158 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 159 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa11+IgG-Fc2a <400> 159 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 160 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa11+IgG-Fc2b <400> 160 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 161 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa11+IgG-Fc2b <400> 161 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 162 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa11+IgG-Fc4a <400> 162 atggccccaa cctcagcctt cttcacagcc ctggtgctgc tcagctacaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctggaatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcatcctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatac atccttctct tcctccacac acctgtaagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 163 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa11+IgG-Fc4a <400> 163 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Tyr 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asn Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Ile Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Thr Ser Phe Ser Ser Ser 165 170 175 Thr His Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 <210> 164 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa112+IgG-Fc1a <400> 164 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagg ctgtgaagtg 660 gccgggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccag 720 acccccgagg tcacgtgcgg tggtggacgt cagcaaggag cacgccgagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggccgagacg agaccaaagg aggagcagtt 840 caacagcacc taccgtgtgg tcagctctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agttacaccc tgcccccacc cgccgaggag 1020 ctgtccagga gcaaagtcac cgtaacctgc ctggtcattg gcttctaccc acctgacatc 1080 catgttgagt ggaagagcaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga ccttcttcct gtacagcaag ctcgcggtgg acaaggcaag 1200 atgggaccat ggagaaacat ttgagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc catctccaag actcagggta aa 1292 <210> 165 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa12+IgG-Fc1a <400> 165 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 166 <211> 1297 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa12+IgG-Fc1b <400> 166 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaataca ccagccgcaa acatgtccca tatgcccagg ctgtgaagtg 660 gccgggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccag 720 acccccgagg tcacgtgcgg gtggtggacg tcagcaagga gcacgccgag gtccagttct 780 cctggtacgt ggacggcgta gaggtgcaca cggccgagac gagaccaaag gaggagcagt 840 tcaacagcac ctaccgtgtg gtcagctcct gcccatccag caccaggact ggctgaaggg 900 gaaggagttc aagtgcaagg tcaacaacgt agacctccca gcccccatca cgaggaccat 960 ctccaaggct atagggcaga gccgggagcc gcagtgtaca ccctgccccc acccgccgag 1020 gagctgtcca ggagcaaagt cacgctaacc tgcctggtca ttggcttcta cccacctgac 1080 atccatgttg agtggaagag caacggacag ccggagccag gaacacatac cgcaccaccc 1140 cgccccagca ggacgtggac gggaccttct tcctgtacag caaactcgcg gtggacaagg 1200 caagatggga ccatggagac aaatttgagt gtgcggtgat gcacgagctc tgcacaacca 1260 ctacacccag aagtccatct ccaagactca gggtaaa 1297 <210> 167 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa12+IgG-Fc1b <400> 167 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 168 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa12+IgG-Fc2a <400> 168 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaatca 660 ccagggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acaccccagg tcacgtgcgg tggttgatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctac ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccaca cgccgaggag 1020 ctgtccagga gcaaagtcag cataacctgc ctggtcattg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ttctcggtgg acaaggccag 1200 ctggcagggt ggaggcatat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc tatctccaag actccgggta aa 1292 <210> 169 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa12+IgG-Fc2a <400> 169 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 170 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa12+IgG-Fc2b <400> 170 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaatcg 660 ccagggccct cggtcttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acaccccagg tcacgtgcgg tagttgatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctgc ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccaca cgccgaggag 1020 ctgtccagga gcaaagtcag cataacctgc ctggtcattg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ttctcggtgg acaaggccag 1200 ctggcagggt ggaggcatat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc tatctccaag actccgggta aa 1292 <210> 171 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa12+IgG-Fc2b <400> 171 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 172 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa12+IgG-Fc4a <400> 172 atggccccaa cctcagcctt cctcacggtc ctggtgctac tcagctgcaa tgccatctgc 60 tgtctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggg ctggaaggga cgcccctgct ggaggaggac tccatcctgg 420 ctgtgaggaa atacttccac agactcaccc tctatctgca agagaagata cagcccctgt 480 gcctgggaga tcgtcagggc agaagtcatg agagtcttct cttcctccac aaacctgcaa 540 gacagactca ggaagaagga gtgaggtggc tcaggtggat ccggtggagg cggaagcggc 600 ggtggaggat caggaacaaa gaccaaacca ccatgtccca tatgcccagc ctgtgaaggg 660 cccgggccct cggccttcat cttccctcca aaacccaagg acaccctcat gatctcccgg 720 acccccaagg tcacgtgcgg tggtagatgt gagccaggag aacccggagg tccagttctc 780 ctggtacgtg gacggcgtag aggtgcacac ggcccagacg aggccaaagg aggagcagtt 840 caacagcacc taccgcgtgg tcagctctgc ccatccagca ccaggactgg ctgaacggga 900 aggagttcaa gtgcaaggtc aacaacaaag acctcccagc ccccatcaca aggatcatct 960 ccaaggccaa agggcagacc cgggagccgc agttacaccc tgcccccacc caccgaggag 1020 ctgtccagga gcaaagtcac gctaacctgc ctggtcactg gcttctaccc acctgacatc 1080 gatgtcgagt ggcaaagaaa cggacagccg gagccagggg aattaccgca ccaccccgcc 1140 ccagcaggac gtggacggga cctacttcct gtacagcaag ctcgcggtgg acaaggccag 1200 ctggcagcgt ggagacacat tccagtgtgc ggtgatgcac gagctctcac aaccactaca 1260 cccagaagtc catcttcaag actccgggta aa 1292 <210> 173 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa12+IgG-Fc4a <400> 173 Met Ala Pro Thr Ser Ala Phe Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 174 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa13+IgG-Fc1a <400> 174 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 175 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa13+IgG-Fc1a <400> 175 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 176 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa13+IgG-Fc1b <400> 176 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 177 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa13+IgG-Fc1b <400> 177 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 178 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa13+IgG-Fc2a <400> 178 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 179 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa13+IgG-Fc2a <400> 179 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 180 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa13+IgG-Fc2b <400> 180 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 181 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa13+IgG-Fc2b <400> 181 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 182 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa13+IgG-Fc4a <400> 182 atggccccat cctcagccct cctcacagcc ctggtgctgc tcagttgcaa tgccatctgc 60 tctctgggct gcaacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcctttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggcgggc tgaaggcacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 183 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa13+IgG-Fc4a <400> 183 Met Ala Pro Ser Ser Ala Leu Leu Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 184 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa14+IgG-Fc1a <400> 184 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 185 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa14+IgG-Fc1a <400> 185 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 186 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa14+IgG-Fc1b <400> 186 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 187 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa14+IgG-Fc1b <400> 187 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 188 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa14+IgG-Fc2a <400> 188 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 189 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa14+IgG-Fc2a <400> 189 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 190 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa14+IgG-Fc2b <400> 190 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 191 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa14+IgG-Fc2b <400> 191 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 192 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa14+IgG-Fc4a <400> 192 atggccccat cctcaactct cctcacggtc ctggtgctgc tcagctgcaa tgccatctgc 60 tgtctgggct gcaacctgcc tcagacccac agcctggctc ataccagggc cctgaggctc 120 ctggcacatg aggagaatct ctcccttctc ctgcctggac cacagaaggg actttggatc 180 ccctcatgag gcttttgggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcataca ggaggcgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcagg agaagaacta cagcctctgt 480 gcctgggaga tcatcagggc agaagcatag agtcttctct tcctccacaa acctgcaaga 540 cagactcagg aagaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 193 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa14+IgG-Fc4a <400> 193 Met Ala Pro Ser Ser Thr Leu Leu Thr Val Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Cys Leu Gly Cys Asn Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Ser Pro His Glu 50 55 60 Ala Phe Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Ile Gln Glu Ala Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Asn Tyr Ser Leu Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Val Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Lys Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 194 <211> 1287 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa15+IgG-Fc1a <400> 194 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtgag gtggctcagg tggatccggt ggaggcggaa gcggcggtgg 600 aggatcagga acaaagacca aaccaccatg tcccatatgc ccaggctgtg aagtggccgg 660 gccctcggtc ttcatcttcc ctccaaaacc caaggacacc ctcatgatct cccagacccc 720 cgaggtcacg tgcggtggtg gacgtcagca aggagcacgc cgaggtccag ttctcctggt 780 acgtggacgg cgtagaggtg cacacggccg agacgagacc aaaggaggag cagttcaaca 840 gcacctaccg tgtggtcagc tctgcccatc cagcaccagg actggctgaa ggggaaggag 900 ttcaagtgca aggtcaacaa cgtagacctc ccagccccca tcacgaggac catctccaag 960 gctatagggc agagccggga gccgcagtta caccctgccc ccacccgccg aggagctgtc 1020 caggagcaaa gtcaccgtaa cctgcctggt cattggcttc tacccacctg acatccatgt 1080 tgagtggaag agcaacggac agccggagcc aggggaatta ccgcaccacc ccgccccagc 1140 aggacgtgga cgggaccttc ttcctgtaca gcaagctcgc ggtggacaag gcaagatggg 1200 accatggaga aacatttgag tgtgcggtga tgcacgagct ctcacaacca ctacacccag 1260 aagtccatct ccaagactca gggtaaa 1287 <210> 195 <211> 430 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa15+IgG-Fc1a <400> 195 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys Pro 195 200 205 Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val Phe 210 215 220 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr Pro 225 230 235 240 Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu Val 245 250 255 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu Thr 260 265 270 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 275 280 285 Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys Cys 290 295 300 Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile Ser 305 310 315 320 Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro Ala 325 330 335 Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile Gly 340 345 350 Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln Pro 355 360 365 Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 370 375 380 Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg Trp 385 390 395 400 Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu His 405 410 415 Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 196 <211> 1292 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa15+IgG-Fc1b <400> 196 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtgag gtggctcagg tggatccggt ggaggcggaa gcggcggtgg 600 aggatcagga atacaccagc cgcaaacatg tcccatatgc ccaggctgtg aagtggccgg 660 gccctcggtc ttcatcttcc ctccaaaacc caaggacacc ctcatgatct cccagacccc 720 cgaggtcacg tgcgggtggt ggacgtcagc aaggagcacg ccgaggtcca gttctcctgg 780 tacgtggacg gcgtagaggt gcacacggcc gagacgagac caaaggagga gcagttcaac 840 agcacctacc gtgtggtcag ctcctgccca tccagcacca ggactggctg aaggggaagg 900 agttcaagtg caaggtcaac aacgtagacc tcccagcccc catcacgagg accatctcca 960 aggctatagg gcagagccgg gagccgcagt gtacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggaaca cataccgcac caccccgccc 1140 cagcaggacg tggacgggac cttcttcctg tacagcaaac tcgcggtgga caaggcaaga 1200 tgggaccatg gagacaaatt tgagtgtgcg gtgatgcacg agctctgcac aaccactaca 1260 cccagaagtc catctccaag actcagggta aa 1292 <210> 197 <211> 430 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa15+IgG-Fc1b <400> 197 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro Gln 195 200 205 Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val Phe 210 215 220 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr Pro 225 230 235 240 Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu Val 245 250 255 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu Thr 260 265 270 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 275 280 285 Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys Cys 290 295 300 Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile Ser 305 310 315 320 Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro Ala 325 330 335 Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile Gly 340 345 350 Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln Pro 355 360 365 Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 370 375 380 Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg Trp 385 390 395 400 Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu His 405 410 415 Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 198 <211> 1287 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa15+IgG-Fc2a <400> 198 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtgag gtggctcagg tggatccggt ggaggcggaa gcggcggtgg 600 aggatcagga acaaagacca aaccaccatg tcccatatgc ccagcctgtg aatcaccagg 660 gccctcggtc ttcatcttcc ctccaaaacc caaggacacc ctcatgatct cccggacacc 720 ccaggtcacg tgcggtggtt gatgtgagcc aggagaaccc ggaggtccag ttctcctggt 780 acgtggacgg cgtagaggtg cacacggccc agacgaggcc aaaggaggag cagttcaaca 840 gcacctaccg cgtggtcagc tctacccatc cagcaccagg actggctgaa cgggaaggag 900 ttcaagtgca aggtcaacaa caaagacctc ccagccccca tcacaaggat catctccaag 960 gccaaagggc agacccggga gccgcagtta caccctgccc ccacacgccg aggagctgtc 1020 caggagcaaa gtcagcataa cctgcctggt cattggcttc tacccacctg acatcgatgt 1080 cgagtggcaa agaaacggac agccggagcc aggggaatta ccgcaccacc ccgccccagc 1140 aggacgtgga cgggacctac ttcctgtaca gcaagttctc ggtggacaag gccagctggc 1200 agggtggagg catattccag tgtgcggtga tgcacgagct ctcacaacca ctacacccag 1260 aagtctatct ccaagactcc gggtaaa 1287 <210> 199 <211> 430 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa15+IgG-Fc2a <400> 199 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys Pro 195 200 205 Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val Phe 210 215 220 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 225 230 235 240 Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val 245 250 255 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr 260 265 270 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 275 280 285 Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 290 295 300 Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser 305 310 315 320 Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His Ala 325 330 335 Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile Gly 340 345 350 Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 355 360 365 Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 370 375 380 Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser Trp 385 390 395 400 Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu His 405 410 415 Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 200 <211> 1287 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa15+IgG-Fc2b <400> 200 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtgag gtggctcagg tggatccggt ggaggcggaa gcggcggtgg 600 aggatcagga acaaagacca aaccaccatg tcccatatgc ccagcctgtg aatcgccagg 660 gccctcggtc ttcatcttcc ctccaaaacc caaggacacc ctcatgatct cccggacacc 720 ccaggtcacg tgcggtagtt gatgtgagcc aggagaaccc ggaggtccag ttctcctggt 780 acgtggacgg cgtagaggtg cacacggccc agacgaggcc aaaggaggag cagttcaaca 840 gcacctaccg cgtggtcagc tctgcccatc cagcaccagg actggctgaa cgggaaggag 900 ttcaagtgca aggtcaacaa caaagacctc ccagccccca tcacaaggat catctccaag 960 gccaaagggc agacccggga gccgcagtta caccctgccc ccacacgccg aggagctgtc 1020 caggagcaaa gtcagcataa cctgcctggt cattggcttc tacccacctg acatcgatgt 1080 cgagtggcaa agaaacggac agccggagcc aggggaatta ccgcaccacc ccgccccagc 1140 aggacgtgga cgggacctac ttcctgtaca gcaagttctc ggtggacaag gccagctggc 1200 agggtggagg catattccag tgtgcggtga tgcacgagct ctcacaacca ctacacccag 1260 aagtctatct ccaagactcc gggtaaa 1287 <210> 201 <211> 430 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa15+IgG-Fc2b <400> 201 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys Pro 195 200 205 Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val Phe 210 215 220 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 225 230 235 240 Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val 245 250 255 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr 260 265 270 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 275 280 285 Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 290 295 300 Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser 305 310 315 320 Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His Ala 325 330 335 Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile Gly 340 345 350 Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 355 360 365 Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 370 375 380 Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser Trp 385 390 395 400 Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu His 405 410 415 Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 202 <211> 1287 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa15+IgG-Fc4a <400> 202 atggccccaa cctcagcctt cctcatggcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gcgacctgcc tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg agaatctccc ctttctcctg cctggaccac agaagggact ttggattccc 180 ccaagaggcc ttggggggca accaggtcca gaaggctcaa gccatggctc tggtgcatga 240 gatgctccag cagcttccag ctcttcagca cagagggctc ggctgctgcc tgggatgaga 300 gcctcctgca ccagttctgc actggactgg atcagcagct cagggacctg gaagcctgtg 360 tcatgcagga ggcggggctg agggaccccc ctgctggagg aggactccat cctggctgtg 420 aggaaatact tccacagact caccctctat ctgcaagaga agagctacag cccctgtgcc 480 tgggagatcg tcagggcaga agtcagaggc cttctcttcc tccacaaacc tgcaagacag 540 actcaggagg aaggagtgag gtggctcagg tggatccggt ggaggcggaa gcggcggtgg 600 aggatcagga acaaagacca aaccaccatg tcccatatgc ccagcctgtg aagggcccgg 660 gccctcggcc ttcatcttcc ctccaaaacc caaggacacc ctcatgatct cccggacccc 720 caaggtcacg tgcggtggta gatgtgagcc aggagaaccc ggaggtccag ttctcctggt 780 acgtggacgg cgtagaggtg cacacggccc agacgaggcc aaaggaggag cagttcaaca 840 gcacctaccg cgtggtcagc tctgcccatc cagcaccagg actggctgaa cgggaaggag 900 ttcaagtgca aggtcaacaa caaagacctc ccagccccca tcacaaggat catctccaag 960 gccaaagggc agacccggga gccgcagtta caccctgccc ccacccaccg aggagctgtc 1020 caggagcaaa gtcacgctaa cctgcctggt cactggcttc tacccacctg acatcgatgt 1080 cgagtggcaa agaaacggac agccggagcc aggggaatta ccgcaccacc ccgccccagc 1140 aggacgtgga cgggacctac ttcctgtaca gcaagctcgc ggtggacaag gccagctggc 1200 agcgtggaga cacattccag tgtgcggtga tgcacgagct ctcacaacca ctacacccag 1260 aagtccatct tcaagactcc gggtaaa 1287 <210> 203 <211> 430 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa15+IgG-Fc4a <400> 203 Met Ala Pro Thr Ser Ala Phe Leu Met Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Ile Ser Pro 35 40 45 Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu Ala 50 55 60 Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val His 65 70 75 80 Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser Ala 85 90 95 Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu Asp 100 105 110 Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Ala Gly Gly 115 120 125 Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe 130 135 140 His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys Ala 145 150 155 160 Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser Thr 165 170 175 Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly Ser 180 185 190 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys Pro 195 200 205 Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala Phe 210 215 220 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 225 230 235 240 Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val 245 250 255 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr 260 265 270 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 275 280 285 Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 290 295 300 Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser 305 310 315 320 Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro Thr 325 330 335 Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr Gly 340 345 350 Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 355 360 365 Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 370 375 380 Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser Trp 385 390 395 400 Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu His 405 410 415 Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 204 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc1a <400> 204 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcggtg gtggacgtca gcaaggagca cgccgaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg ccgagacgag accaaaggag gagcagttca 840 acagcaccta ccgtgtggtc agctctgccc atccagcacc aggactggct gaaggggaag 900 gagttcaagt gcaaggtcaa caacgtagac ctcccagccc ccatcacgag gaccatctcc 960 aaggctatag ggcagagccg ggagccgcag ttacaccctg cccccacccg ccgaggagct 1020 gtccaggagc aaagtcaccg taacctgcct ggtcattggc ttctacccac ctgacatcca 1080 tgttgagtgg aagagcaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc ttcttcctgt acagcaagct cgcggtggac aaggcaagat 1200 gggaccatgg agaaacattt gagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tctccaagac tcagggtaaa 1290 <210> 205 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa16+IgG-Fc1a <400> 205 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Val Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Glu Thr Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 206 <211> 1295 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc1b <400> 206 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaatacacc agccgcaaac atgtcccata tgcccaggct gtgaagtggc 660 cgggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccagac 720 ccccgaggtc acgtgcgggt ggtggacgtc agcaaggagc acgccgaggt ccagttctcc 780 tggtacgtgg acggcgtaga ggtgcacacg gccgagacga gaccaaagga ggagcagttc 840 aacagcacct accgtgtggt cagctcctgc ccatccagca ccaggactgg ctgaagggga 900 aggagttcaa gtgcaaggtc aacaacgtag acctcccagc ccccatcacg aggaccatct 960 ccaaggctat agggcagagc cgggagccgc agtgtacacc ctgcccccac ccgccgagga 1020 gctgtccagg agcaaagtca cgctaacctg cctggtcatt ggcttctacc cacctgacat 1080 ccatgttgag tggaagagca acggacagcc ggagccagga acacataccg caccaccccg 1140 ccccagcagg acgtggacgg gaccttcttc ctgtacagca aactcgcggt ggacaaggca 1200 agatgggacc atggagacaa atttgagtgt gcggtgatgc acgagctctg cacaaccact 1260 acacccagaa gtccatctcc aagactcagg gtaaa 1295 <210> 207 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc1b <400> 207 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ile His Gln Pro 195 200 205 Gln Thr Cys Pro Ile Cys Pro Gly Cys Glu Val Ala Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Gln Thr 225 230 235 240 Pro Glu Val Thr Cys Val Val Val Asp Val Ser Lys Glu His Ala Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Glu 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Lys Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Val Asp Leu Pro Ala Pro Ile Thr Arg Thr Ile 305 310 315 320 Ser Lys Ala Ile Gly Gln Ser Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile His Val Glu Trp Lys Ser Asn Gly Gln 355 360 365 Pro Glu Pro Glu Asn Thr Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Phe Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Arg 385 390 395 400 Trp Asp His Gly Asp Lys Phe Glu Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Gln Gly Lys 420 425 430 <210> 208 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc2a <400> 208 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcacc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggtg gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctaccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 209 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa16+IgG-Fc2a <400> 209 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 210 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc2b <400> 210 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaatcgcc 660 agggccctcg gtcttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 accccaggtc acgtgcggta gttgatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccacacg ccgaggagct 1020 gtccaggagc aaagtcagca taacctgcct ggtcattggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagtt ctcggtggac aaggccagct 1200 ggcagggtgg aggcatattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcta tctccaagac tccgggtaaa 1290 <210> 211 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa16+IgG-Fc2b <400> 211 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro His 325 330 335 Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 420 425 430 <210> 212 <211> 1290 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa16+IgG-Fc4a <400> 212 atggccccaa cctcagcctt cttcacggcc ctggtgctgc tcagctgcaa tgccatctgc 60 tctctgggct gcgacccggt tcagacccac agcctggctc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacg cccctgctgg aggagggctc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcatcagggc agaagcatag agccttctct tcctccacaa acctgcaaga 540 cagactcagg aggaaggagt gaggtggctc aggtggatcc ggtggaggcg gaagcggcgg 600 tggaggatca ggaacaaaga ccaaaccacc atgtcccata tgcccagcct gtgaagggcc 660 cgggccctcg gccttcatct tccctccaaa acccaaggac accctcatga tctcccggac 720 ccccaaggtc acgtgcggtg gtagatgtga gccaggagaa cccggaggtc cagttctcct 780 ggtacgtgga cggcgtagag gtgcacacgg cccagacgag gccaaaggag gagcagttca 840 acagcaccta ccgcgtggtc agctctgccc atccagcacc aggactggct gaacgggaag 900 gagttcaagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag gatcatctcc 960 aaggccaaag ggcagacccg ggagccgcag ttacaccctg cccccaccca ccgaggagct 1020 gtccaggagc aaagtcacgc taacctgcct ggtcactggc ttctacccac ctgacatcga 1080 tgtcgagtgg caaagaaacg gacagccgga gccaggggaa ttaccgcacc accccgcccc 1140 agcaggacgt ggacgggacc tacttcctgt acagcaagct cgcggtggac aaggccagct 1200 ggcagcgtgg agacacattc cagtgtgcgg tgatgcacga gctctcacaa ccactacacc 1260 cagaagtcca tcttcaagac tccgggtaaa 1290 <210> 213 <211> 431 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa16+IgG-Fc4a <400> 213 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Pro Val Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Gly Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Ile Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Thr Asn Leu Gln Asp Arg Leu Arg Arg Lys Glu Gly Gly Ser Gly Gly 180 185 190 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Thr Lys Thr Lys 195 200 205 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro Ser Ala 210 215 220 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 225 230 235 240 Pro Lys Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 245 250 255 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 260 265 270 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 275 280 285 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 290 295 300 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 305 310 315 320 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Tyr Thr Leu Pro Pro Pro 325 330 335 Thr Glu Glu Leu Ser Arg Ser Lys Val Thr Leu Thr Cys Leu Val Thr 340 345 350 Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 355 360 365 Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val 370 375 380 Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val Asp Lys Ala Ser 385 390 395 400 Trp Gln Arg Gly Asp Thr Phe Gln Cys Ala Val Met His Glu Ala Leu 405 410 415 His Asn His Tyr Thr Gln Lys Ser Ile Phe Lys Thr Pro Gly Lys 420 425 430 <210> 214 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa17+IgG-Fc1a <400> 214 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcggtggtgg 720 acgtcagcaa ggagcacgcc gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccga gacgagacca aaggaggagc agttcaacag cacctaccgt gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaag gggaaggagt tcaagtgcaa ggtcaacaac 900 gtagacctcc cagcccccat cacgaggacc atctccaagg ctatagggca gagccgggag 960 ccgcagttac accctgcccc cacccgccga ggagctgtcc aggagcaaag tcaccgtaac 1020 ctgcctggtc attggcttct acccacctga catccatgtt gagtggaaga gcaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggaccttct 1140 tcctgtacag caagctcgcg gtggacaagg caagatggga ccatggagaa acatttgagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctc caagactcag 1260 ggtaaa 1266 <210> 215 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa17+IgG-Fc1a <400> 215 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Val Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Glu Thr Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 216 <211> 1271 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa17+IgG-Fc1b <400> 216 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa tacaccagcc 600 gcaaacatgt cccatatgcc caggctgtga agtggccggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccagaccccc gaggtcacgt gcgggtggtg 720 gacgtcagca aggagcacgc cgaggtccag ttctcctggt acgtggacgg cgtagaggtg 780 cacacggccg agacgagacc aaaggaggag cagttcaaca gcacctaccg tgtggtcagc 840 tcctgcccat ccagcaccag gactggctga aggggaagga gttcaagtgc aaggtcaaca 900 acgtagacct cccagccccc atcacgagga ccatctccaa ggctataggg cagagccggg 960 agccgcagtg tacaccctgc ccccacccgc cgaggagctg tccaggagca aagtcacgct 1020 aacctgcctg gtcattggct tctacccacc tgacatccat gttgagtgga agagcaacgg 1080 acagccggag ccaggaacac ataccgcacc accccgcccc agcaggacgt ggacgggacc 1140 ttcttcctgt acagcaaact cgcggtggac aaggcaagat gggaccatgg agacaaattt 1200 gagtgtgcgg tgatgcacga gctctgcaca accactacac ccagaagtcc atctccaaga 1260 ctcagggtaa a 1271 <210> 217 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa17+IgG-Fc1b <400> 217 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Ile His Gln Pro Gln Thr Cys Pro Ile Cys Pro Gly 195 200 205 Cys Glu Val Ala Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Gln Thr Pro Glu Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Lys Glu His Ala Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Glu Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Lys Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Val Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Thr Ile Ser Lys Ala Ile Gly Gln Ser Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile His 340 345 350 Val Glu Trp Lys Ser Asn Gly Gln Pro Glu Pro Glu Asn Thr Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Phe Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Arg Trp Asp His Gly Asp Lys Phe Glu 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Gln Gly Lys 420 <210> 218 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa17+IgG-Fc2a <400> 218 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcaccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtggttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctacccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 219 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa17+IgG-Fc2a <400> 219 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 220 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa17+IgG-Fc2b <400> 220 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga atcgccaggg ccctcggtct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggacaccc caggtcacgt gcggtagttg 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacacgccga ggagctgtcc aggagcaaag tcagcataac 1020 ctgcctggtc attggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagttctcg gtggacaagg ccagctggca gggtggaggc atattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtctatctc caagactccg 1260 ggtaaa 1266 <210> 221 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa17+IgG-Fc2b <400> 221 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Ser Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Phe Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Ser Lys Thr Pro Gly Lys 420 <210> 222 <211> 1266 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence of interferon recombinant protein INFa17+IgG-Fc4a <400> 222 atggccccaa cctcagcctt cttcacagcc ctggtgctac tcagctgcaa tgccatctgc 60 tctctgggct gtgacctgcc tcagacccat agcctggcgc acaccagggc cctgaggctc 120 ctggcacatg aggagaatct cccccttctc ctgcctggac cacagaaggg actttggatt 180 cccccaagag gccttggggg gcaaccaggt ccagaaggct caagccatgg ctctggtgca 240 tgagatgctc cagaaccttc cagctcttca gcacagaggg ctcggctgct gcctgggatg 300 agagcctcct gcaccagttc tgcactggac tggatcagca gctcagggac ctggaagcct 360 gtgtcatgca ggaggtgggc tgaagggacc cccctgctgg aggaggactc catcctggct 420 gtgaggaaat acttccacag actcaccctc tatctgcaag agaagagcta cagcccctgt 480 gcctgggaga tcgtcagggc agaagcatag agccttctct tcctccagaa acctgtgagg 540 tggctcaggt ggatccggtg gaggcggaag cggcggtgga ggatcaggaa caaagaccaa 600 accaccatgt cccatatgcc cagcctgtga agggcccggg ccctcggcct tcatcttccc 660 tccaaaaccc aaggacaccc tcatgatctc ccggaccccc aaggtcacgt gcggtggtag 720 atgtgagcca ggagaacccg gaggtccagt tctcctggta cgtggacggc gtagaggtgc 780 acacggccca gacgaggcca aaggaggagc agttcaacag cacctaccgc gtggtcagct 840 ctgcccatcc agcaccagga ctggctgaac gggaaggagt tcaagtgcaa ggtcaacaac 900 aaagacctcc cagcccccat cacaaggatc atctccaagg ccaaagggca gacccgggag 960 ccgcagttac accctgcccc cacccaccga ggagctgtcc aggagcaaag tcacgctaac 1020 ctgcctggtc actggcttct acccacctga catcgatgtc gagtggcaaa gaaacggaca 1080 gccggagcca ggggaattac cgcaccaccc cgccccagca ggacgtggac gggacctact 1140 tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac acattccagt 1200 gtgcggtgat gcacgagctc tcacaaccac tacacccaga agtccatctt caagactccg 1260 ggtaaa 1266 <210> 223 <211> 423 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of interferon recombinant protein INFa17+IgG-Fc4a <400> 223 Met Ala Pro Thr Ser Ala Phe Phe Thr Ala Leu Val Leu Leu Ser Cys 1 5 10 15 Asn Ala Ile Cys Ser Leu Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Ala His Thr Arg Ala Leu Arg Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Pro Phe Ser Cys Leu Asp His Arg Arg Asp Phe Gly Phe Pro Gln Glu 50 55 60 Ala Leu Gly Gly Asn Gln Val Gln Lys Ala Gln Ala Met Ala Leu Val 65 70 75 80 His Glu Met Leu Gln Gln Thr Phe Gln Leu Phe Ser Thr Glu Gly Ser 85 90 95 Ala Ala Ala Trp Asp Glu Ser Leu Leu His Gln Phe Cys Thr Gly Leu 100 105 110 Asp Gln Gln Leu Arg Asp Leu Glu Ala Cys Val Met Gln Glu Val Glu 115 120 125 Gly Thr Pro Leu Leu Glu Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 130 135 140 Phe His Arg Leu Thr Leu Tyr Leu Gln Glu Lys Ser Tyr Ser Pro Cys 145 150 155 160 Ala Trp Glu Ile Val Arg Ala Glu Val Met Arg Ala Phe Ser Ser Ser 165 170 175 Arg Asn Leu Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 180 185 190 Gly Gly Ser Gly Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala 195 200 205 Cys Glu Gly Pro Gly Pro Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys 210 215 220 Asp Thr Leu Met Ile Ser Arg Thr Pro Lys Val Thr Cys Val Val Val 225 230 235 240 Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp 245 250 255 Gly Val Glu Val His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe 260 265 270 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp 275 280 285 Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 290 295 300 Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg 305 310 315 320 Glu Pro Tyr Thr Leu Pro Pro Pro Thr Glu Glu Leu Ser Arg Ser Lys 325 330 335 Val Thr Leu Thr Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp 340 345 350 Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg 355 360 365 Thr Thr Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser 370 375 380 Lys Leu Ala Val Asp Lys Ala Ser Trp Gln Arg Gly Asp Thr Phe Gln 385 390 395 400 Cys Ala Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 405 410 415 Ile Phe Lys Thr Pro Gly Lys 420 1

Claims

동물 인터페론 및 동물 면역글로불린 Fc 단편을 포함하는 동물용 재조합 융합 인터페론.
제1항에 있어서, 동물 인터페론 및 동물 면역글로불린 Fc 단편이 링커에 의해 연결된 동물용 재조합 융합 인터페론.
제1항 또는 제2항에 있어서, 동물 인터페론이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:2, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 및 47로 구성된 군으로부터 선택된 적어도 하나의 서열인 동물용 재조합 융합 인터페론.
제1항 또는 제2항에 있어서, 동물 인터페론이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:2, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 및 47로 구성된 군으로부터 선택된 적어도 하나의 서열과 적어도 80%의 상동성을 갖는 동물용 재조합 융합 인터페론.
제1항 또는 제2항에 있어서, 동물 면역글로불린 Fc 단편이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:4, 49, 51, 53, 및 55로 구성된 군으로부터 선택된 적어도 하나의 서열인 동물용 재조합 융합 인터페론.
제1항 또는 제2항에 있어서, 동물 면역글로불린 Fc 단편이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:4, 49, 51, 53, 및 55로 구성된 군으로부터 선택된 적어도 하나의 서열과 적어도 80%의 상동성을 갖는 동물용 재조합 융합 인터페론.
제2항에 있어서, 링커가 SEQ ID NO:6의 서열을 포함하는 동물용 재조합 융합 인터페론.
제2항에 있어서, 링커가 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:6의 서열과 적어도 80%의 상동성을 갖는 동물용 재조합 융합 인터페론.
제2항에 있어서, 동물용 재조합 융합 인터페론이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223으로 구성된 군으로부터 선택되는 동물용 재조합 융합 인터페론.
제2항에 있어서, 동물용 재조합 융합 인터페론이 아미노산 서열을 포함하고, 아미노산 서열이 SEQ ID NO:8, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 및 223으로 구성된 군으로부터 선택된 서열과 적어도 80%의 상동성을 갖는 동물용 재조합 융합 인터페론.
제1항의 동물용 재조합 융합 인터페론을 인코딩하는 폴리뉴클레오티드.
제11항의 폴리뉴클레오티드를 갖는 포유동물 세포를 혈청-함유 배지에서 배양하는 단계;
세포가 안정적으로 성장한 후에 혈청-함유 배지를 혈청-비함유 배지로 대체하는 단계; 및
1 내지 5일마다 혈청-비함유 배지를 수거하고, 새로운 혈청-비함유 배지를 세포에 첨가하는 단계를 포함하는 동물용 재조합 융합 인터페론을 생성시키는 방법.
제12항에 있어서, 혈청-함유 배지가 약 0.1 내지 10%(v/v)의 혈청을 포함하는 방법.
동물용 항바이러스 약제의 제조를 위한 제1항의 동물용 재조합 융합 인터페론의 용도.