KR20140128713A - Perfume Composition Using an Essential Oil Extract of Acorus gramineus and an Essential Oil Extract of Torreya nucifera - Google Patents

Abstract

Disclosed in the present invention are a perfume composition using an essential oil extract of Acorus gramineus and an essential oil extract of Torreys nucifera. Specifically, disclosed in the present invention is a perfume composition using an essential oil extract of Acorus gramineus and an essential oil extract of Torreys nucifera having excellent fragrance properties and stress resistance activity.

Description

(Perfume Composition Using an Essential Oil Extract of Acorus gramineus and an Essential Oil Extract of Torreya nucifera)

The invention seokchangpo (Acorus gramineus ) essential oil extract and Torreya nucifera immature and essential oil extract.

Spices are used for cosmetics such as perfumes and perfumes which are widely used in our daily life, food products such as fragrances, foods such as drinks, quasi-drugs such as toothpaste, medicines such as ointments, and the like, There are also functionalities such as mind and body relaxation effect.

Spices are divided into natural fragrances and synthetic fragrances. Most fragrances currently used are synthetic fragrances. However, the chemical synthetic perfume does not give a lasting affinity to the user, and the additive used for long lasting fragrance may cause side effects such as allergic reaction. Recently, interest in perfumes using natural essential oils obtained from plants is increasing.

Natural essential oils are essential oils. Essential oils are mono-terpenes, sesquiterpenes, and other volatile oils. Flowers, leaves, stems, and roots of plants with aroma are distilled, pressed, Lt; / RTI >

Recently, consumption tendency is not only a divergence of fragrance but also a product having functionality. Therefore, research on plant essential oil having excellent perfume and functionality is active.

Korean Patent Publication No. 2010-0124620 discloses a perfume composition having a sleeping effect using rose oil, sandalwood oil, orange flower oil, and ylang ylang. Korean Patent No. 843680 discloses a perfume composition containing lemon essential oil, peppermint essential oil, And Korean Patent No. 843692 discloses a fragrance composition having a psycho-sedative effect using lemon essential oil, orange essential oil, lavender essential oil, lemon grass essential oil, and the like. In addition, natural flavors having antioxidant effect (Korean Patent Publication No. 2011-0060602) and preservative effect (Korean Patent Publication No. 20120025688) have been proposed as functionalities.

The present invention discloses a perfume composition having anti-stress activity using an extract of Seok Changpo oil and essential oil extract of Visa tree.

It is an object of the present invention to provide a fragrance composition using an extract of essential oil of Seok Changpo and an extract of essential oil of Viza.

Another object of the present invention is to provide an anti-stress composition using an extract of Seokchangpo essential oil and an essential oil extract of Visa tree.

Other and further objects of the present invention will be described below.

As shown in the following examples and experimental examples, the inventors of the present invention found that the essential oil extracts of roots and stalks of Seokchangpo, the immature and viscous oil extracts of Viza japonica have excellent flavor, low state anxiety index (STAI-KYZ I) In animal models, the concentration of cortisol was lowered. The STAI-KYZ (State Trait Anxiety Inventory) was developed to distinguish between the concept of stress and anxiety, and to distinguish individual anomalies of trait anxiety, which is a temporary emotional state, and relatively stable personality trait, The results of this study are as follows. First, the study was designed to reflect the personality type of Korean people. The higher the score, the higher the level of anxiety (Study of Student Guidance 10: 214, 1993; Journal of the Korean Psychology, Health 1: Cortisol is a hormone secreted into the blood to respond to stress when stress is applied and is known to be an objective marker of stress level (Korean Psychological Association, Health, 9 (3): 633-645, 2004).

The present invention is provided based on the above-described experimental results, and the perfume composition of the present invention is characterized in that it contains an essential oil extract of roots and / or stems of Seokchangpo and / or an immature and viscous oil extract of Visa wood.

In the present specification, the term "fragrance" is used to provide a beneficial taste to a human being to feel a refreshing feeling and an exhilarating feeling, or to provide a beneficial function to human beings such as a mind and body soothing effect, .

In the present specification, the term " visa tree seedless "means fruit and peel of the visa tree fruit before harvest (normally, visa refers to visa tree fruit), and specifically, In terms of being composed of seed coat, it can be said that seed coat before harvest (meaning seed coat contains flesh and peel). More specifically, considering that the ripening in the harvesting stage has the characteristic of splitting (splitting) of the seeds, it is said that the seeds of the seeds of the seeds of the seeds of Viza japonica .

In the present specification, the term "essential oil extract" refers to an extract of an oil-phase obtained by immersing a perfume ingredient in a viscera tree stem or immature seed which is an object to be extracted. Examples of the essential oil extracts include water vapor distillation method, solvent extraction method using volatile solvent (benzene, hexane, etc.), leaching method (method of collecting leachate by scratching the extraction target), compression method, supercritical extraction method Method can be used.

The perfume composition of the present invention may contain at least one of the extract of Seokchangpo root / stem essential oil, the immature visa tree and the essential oil extract. However, as shown in the following Examples and Experimental Examples, the fragrance composition of Seokchangpo root / stem essential oil extract, When the essential oil extract is used together, it has excellent perfume properties and excellent effect of lowering the state anxiety index (STAI-KYZ I). Therefore, the perfume composition of the present invention is characterized in that it contains extracts of rosewood / stem essential oil, Are preferably included.

The perfume composition of the present invention may further contain a preservative to maintain the quality of the perfume composition. Such a preservative may be an antioxidant to prevent oxidative damage, a preservative to prevent decay by bacteria, a light stabilizer to prevent deterioration by light, and the like. These antioxidants, preservatives, light stabilizers, Any of those known in the art can be selected and used in an amount suitable for its use. For example, when tocopherol is used as an antioxidant, the amount to which it is added varies depending on the amount of the ingredient to be sourced in the perfume ingredient, but it may be generally used in the range of 0.01 wt% to 5.0 wt%.

The perfume composition of the present invention is a material used in a product to provide a beneficial flavor to human beings by using it or to provide human-beneficial functionalities such as mind and body stability and the like. Therefore, all the fragrant products, In addition, the fragrance composition of the present invention can be used in all the fragrance products having beneficial psycho-sedative effects.

Examples of such smell-resistant products include fragrances, cosmetics (lipsticks, perfumes, etc.), soaps, hair treatment compositions (hair creams), body care compositions, bath salts, human (hand and face) cleansers, kitchen dishwashers, disinfectant cleaners, But are not limited to, wet tissues and oral compositions (shampoo, toothpaste, gagrin, etc.), medicines (ointments, etc.), foods (beverages, etc.)

The perfume composition of the present invention may contain 0.01% to 99.99% by weight, depending on the purpose for which it is used in the above listed products in which it is used. For example, when a wet tissue product is prepared using the perfume composition of the present invention, the perfume composition of the present invention will usually be contained in the wet tissue product in the range of 0.01 wt% to 3.0 wt%.

Typical examples of products in which the perfume composition of the present invention is used are fragrances.

When the perfume composition of the present invention is used in a perfume product, the perfume composition may contain the perfume composition of the present invention in an amount ranging from 0.01% by weight to 20.0% by weight, particularly 0.01% by weight to 5.0% by weight, , A surfactant, an emulsifier, a volatile accelerator, and the like, and may be prepared into a liquid formulation, a gel formulation or a solid formulation.

Examples of the gelling agent include carrageenan, agar, gelatin, and gellan gum, and may be used in an amount of 0.1 to 20% by weight based on the total weight of the perfume composition of the present invention.

Also, the polymer is used for regulating volatilization for continuous and constant smell diffusion. At least one selected from the group consisting of acryl, amide, vinyl alcohol polymer and copolymers thereof can be used. Specifically, acrylate Acrylate / C10-30 alkyl acrylate crosspolymer, sodium polyacrylate, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, ammonium acryloyldimethyltaurate / vinylpyrrolidone polymer, Polyacrylamide / C13-14 isoparaffin / laureth-7, and isobutylene / maleic acid anhydride copolymer. Such a polymer may be included in the perfume composition of the present invention in the range of 0.1 to 10% by weight based on the total weight of the perfume composition. If it is contained in an amount of less than 0.01% by weight, the volatilization may be too fast, and if it is contained in an amount of 10.0% by weight or more, the volatilization may be too slow.

In addition, the surfactant may be sodium fatty acid, monoalkylsulfate, alkylpolyoxyethylene sulfate, alkylbenzenesulfonate, monoalkyltrimethylammonium salt, dialkyldimethylammonium salt and the like to maintain the phase stability and to sustain the continuous flavor. Such a surfactant may be used in an amount of 0.1 to 10.0% by weight based on the total weight of the perfume composition of the present invention.

The emulsifier may be any conventional solubilizer known in the art which is used to solubilize the perfume. Examples of such emulsifiers include sorbitan sesquioleate, glyceryl monostearate, polyoxyethylene sorbitan tristearate, polyoxyethylene sorbitan trioleate, polyoxyethylene glycol monostearate, polysorbate, polyoxyethylene mono Stearate, polyoxyethylene hydrogenated castor oil (especially PEG-60 Hydorgenated Castor Oil), and the like. Such an emulsifier will usually be contained in the perfume composition of the present invention in the range of 0.1 to 10.0% by weight based on the total weight of the composition.

As the above-mentioned vapor accelerator, for example, ethanol, propylene glycol, dipropylene glycol, ethylene glycol, butylene glycol, and denatured alcohol (Alcohol Denat. Cas No. 64-17-5) may be used. Such a volatile accelerator may be used in the range of 3 wt% to 35 wt% based on the total weight of the perfume composition of the present invention.

Other types of products in which the perfume composition of the present invention is used include cosmetics.

When the perfume composition of the present invention is used in a cosmetic composition, it may be contained in the range of 0.01% to 0.5% by weight based on the total weight of the cosmetic composition, and the cosmetic composition may contain components commonly used in cosmetic formulations.

Examples of such components include inorganic powders and organic powders (e.g., homopolymers, copolymers, and resins). Particularly, talc, sericite, mica, kaolin, silicone resin, nylon and the like are preferable as such powder because of their excellent feel. The average particle size of these powders is preferably from 0.1 to 15 탆, particularly from 1 to 10 탆. Considering the feeling of use, the powder may be added in an amount of 1 to 60% by weight, especially 5 to 25% by weight, based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention may also contain an oily substance. Preferred oily substances from the viewpoint of feeling in use are avocado oil, sucky oil, turtle oil, corn oil, olive oil, wheat germ oil, soybean oil, jojoba oil, peanut oil, cocoa butter, lanolin, liquid paraffin, squalane, squalane, Stearyl ester, and silicone oil. The preferred content range of such an oily substance is 0.1 to 20% by weight, particularly 1 to 10% by weight.

The cosmetic composition of the present invention may also contain an alcohol. Preferred alcohols include ethyl alcohol, propylene glycol, 1,3-butylene glycol, glycerol, sorbitol and the like. The alcohols can be used alone or as a mixture of two or more. In terms of feeling on use, the alcohol may be added in an amount of 0.1 to 30% by weight, particularly 1 to 20% by weight, based on the total weight of the composition for external application for skin of the present invention.

The cosmetic composition of the present invention may further contain other functional substances such as a moisturizing agent, an anti-inflammatory agent, a whitening agent, an ultraviolet absorber, a bactericide, a limiting agent and the like.

The cosmetic compositions of the present invention may be in any form and may be in the form of lotions, emulsions, creams, powders, poultices, packs, massagers, gels, pastes, sprays and the like.

Another type of product in which the perfume composition of the present invention is used is typically a bath product.

When the perfume composition of the present invention is used in the bath composition, it may be contained in an amount ranging from 0.01% to 3.0% by weight based on the total weight of the bath composition, and the bath composition may contain components conventionally used in bath formulation, Organic acids, oily components, and the like.

Examples of inorganic salts include sodium chloride, sodium hydrogen carbonate, sodium carbonate, borax, sodium sulfate, sodium sulfide, sodium sesquicarbonate, sodium nitrate, sodium thiosulfate, sodium polyphosphate, sodium phosphate, calcium oxide, magnesium oxide, calcium carbonate, magnesium carbonate, Potassium, and the like, which may be used alone or as a mixture of two or more. These inorganic salts may be added to the bathing agent in an amount of 5% by weight or more, preferably 10% by weight or more, based on the total weight of the bathing agent.

Examples of the organic acid include succinic acid, fumaric acid, malic acid, tartaric acid, citric acid, benzoic acid, etc. These may be used alone or in a mixture of two or more.

These organic acids may be added to the bathing agent in the range of 0.1 to 50% by weight based on the total weight of the bathing agent.

Examples of the oily component include wax, hydrocarbon, higher fatty acid, higher alcohol, ester, silicone oil and the like.

The bath salts may also contain other ingredients commonly used in the art. These components include (a) inorganic acids such as boric acid, meta silicic acid, and silicic anhydride; (b) herbal medicine powders such as fenugreek, bank, ginger, citrus peel, ginger root, peppermint, ginseng and oats; (c) (D) vitamins such as vitamin A, vitamin C, vitamin D and vitamin E, (e) sulfur, mica powder, white clay powder, Loess powder, rice bran carbide, fungicide, preservative and the like.

Such a bathing agent may be prepared by any desired means such as granules, tablets, liquids, powders, and the like.

As described above, the perfume composition of the present invention can be used as a representative example of fragrance compositions, cosmetics and bath salts. Therefore, the perfume composition of the present invention should not be construed as being limited thereto, It is understood that the perfume composition of the present invention can be used in any and all of the fragrance products as long as it can provide favorable fragrances to humans or can enhance the favorability and utility of the fragrance by providing human- .

In another aspect, the present invention relates to an anti-stress composition.

The anti-stress composition of the present invention is characterized in that it contains an essential oil extract of roots and / or stalks and / or an immature and viscous oil extract of Visa wood as an active ingredient.

In the present specification, "anti-stress" means restoration of mental fatigue, specifically, relaxation, anxiety, or mind-body relaxation.

The anti-stress composition of the present invention may contain an effective amount of the active ingredient in an arbitrary amount (effective amount) as long as it exhibits antistress activity, depending on the purpose of use, formulation, compounding purpose and the like. By weight based on the total weight of the composition. Herein, "effective amount" refers to the amount of active ingredient capable of inducing an anti-stress effect. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The anti-stress composition of the present invention can be identified as a food composition in a specific embodiment.

When the antistress composition of the present invention is identified as a food composition, the form of the food can be selected from the group consisting of tea, juice, carbonated beverage, beverage such as ionic beverage, milk processed, processed oil such as root, gum, rice cake, , Foods such as cotton, powders, tablets, capsules, and the like.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Preservatives that can be used include calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

The anti-stress composition of the present invention can be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may be formulated into a wide variety of dosage forms, including pharmaceutically acceptable carriers, excipients and the like, in addition to the active ingredient, and may be formulated into a wide variety of dosage forms such as a localized preparation such as cream, lotion, ointment (semi-solid external drug), micro loo emulsion, (TTS) (e.g., patches, bandages, etc.), and the like.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (e.g. corn starch, potato starch, etc.), cellulose, derivatives thereof (e.g. sodium carboxymethylcellulose, ethylcellulose, etc.), malt, gelatin, talc, (E.g., peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g., propylene glycol, glycerin and the like), alginic acid, and the like. Depending on the formulation of the pharmaceutical composition of the present invention, one or more suitable ones may be selected and used. Suitable pharmaceutically acceptable carriers and formulations are described in Remington's Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention may further comprise an emulsifying agent (e.g., TWEENS), a wetting agent (e.g., sodium lauryl sulfate), a coloring agent, a flavoring agent, a stabilizer, a preservative, water, saline, a phosphate buffer solution and the like.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

As described above, according to the present invention, it is possible to provide a fragrance composition using an extract of Seok Changpo oil and a extract of Vijayob essential oil. The perfume composition of the present invention can be used in a product which is beneficial to fragrance and has a fragrance as well as a fragrance composition of the present invention which is beneficial for the mind and body soothing effect. Specifically, fragrance composition, cosmetics (lipstick, perfume, etc.) (Such as shampoo, toothpaste, and gagrin), medicines (such as ointments), foods (eg, hair care products), body care compositions, bath preparations, human (hand and face) cleaners, kitchen dishwashing agents, disinfectant cleaners, shower foams, (Beverages, etc.).

Also, according to the present invention, it is possible to provide a composition for anti-stress using a crude oil extract of Seok Changpo and a non-essential oil extract of Visa. The anti-stress composition of the present invention can be commercialized as food or medicine.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Seokchangpo  Preparation of Root Essential Oil Extract and Visa Immature and Essential Oil Extract

&Lt; Example 1 & gt ; Preparation of extracts of roots and stems of Seokchangpo

(SFE500R1, EYESEL CO., LTD.) Using supercritical carbon dioxide as a supercritical fluid. The supernatant was extracted under the conditions of pressure of 350 bar, temperature of 60 ℃, Carbon dioxide flow rate of 3.0 ml / min, and extraction time of 3 hours.

<Example 2> Preparation of visa immature and essential oil extracts

Essential oil extracts were prepared from the flesh and peel of the immature seeds of Viza japonica using the same method as in Example 1 above.

< Manufacturing example > Preparation of a perfume composition

Three fragrance compositions were prepared as fragrance compositions with the ingredients and contents in Table 1 below. &Lt; Preparation Example 1 > contains the essential oil extract of Example 1, Preparation Example 2 contains the non-essential oil extract of Example 2, and Preparation Example 3 contains the extract of Example 1 &Gt; and an equal amount of the visa essential oil extract of &lt; Example 2 &gt;.

Composition and content of the perfume composition division ingredient Content (% by weight)
A phase Purified water 25.40 Isobutylene-maleic acid anhydride copolymer 1.00 B phase Purified water 9.00

C phase Alcohol Denat. 30.00 PIGGY-60 Hydrogenated Castor Oil
(PEG-60 Hydorgenated Castor Oil) 5.00 A mixture of the oil or example oil of each example 5.00
D phase Gelatin (Gellatin) 1.50 Purified water 23.10

The preparation of the perfume composition was carried out by first thoroughly stirring the A-phase, swelling the B-phase, diluting the B-phase into the A-phase, mixing the C-phase homogeneously mixed therein and stirring the mixture. The D phase, in which the gelatin was completely dissolved, was cooled to 45 ° C by heating to 80 ° C, and the mixture was added to the mixture of A phase, B phase and C phase, stirred uniformly and then aged for 1 day.

< Experimental Example > In fragrance  Sensory evaluation, state anxiety test and Antistress  Animal experiments for activity

<Experimental Example 1> Sensory evaluation on flavor

The sensory evaluation was carried out on 20 agents who fully understood the purpose of the experiment on the perfume composition prepared in each of the above Preparation Examples.

The perfume composition prepared in the above Preparation Example was placed in a 10 ml glass bottle and each 2 ml of the perfume composition was presented to the panels at room temperature. The satisfaction of each sample was evaluated by the 5-point scale method (5 points: very good, 4 points: : Normal, 2 points: bad, 1 point: very bad).

The results are shown in Table 2 below as average values.

Sensory evaluation result division Sensory evaluation mean value Production Example 1 3.74 Production Example 2 3.62 Production Example 3 4.12

The results of the above Table 2 show that the perfume composition containing the extract of Seok Changpo and / or the visa essential oil extract of the present invention was generally excellent in general, and in particular, the combination of Seok Changpo oil extract and Visa essential oil extract And that it is better evaluated.

<Experimental Example 2> anxiety test

(STAI-KYZI) was measured before and after inhalation of essential oil extracts.

The questionnaire of STAI-KYZ was made before and after the inhalation of the essential oil extract of the Example. The questionnaire to measure STAI-S consisted of 20 items, and each item was rated as 'not at all', 'somewhat true', 'fairly true', or 'very true' . The sum of the scores is between 20 and 80, and the higher the score, the higher the level of anxiety.

Inhalation of the essential oil extract of the Examples was carried out for 20 minutes through an incense lamp (containing 10% (v / v) essential oil extract).

The results are shown in Table 3 below. Statistical analysis was performed using SAS (version 9.1)

STAI-S score before and after inhalation division Before inhalation After inhalation &Lt; Example 1 > 39.8 ± 3.23 34.6 ± 2.63 * &Lt; Example 2 > 40.7 ± 3.62 35.4 ± 4.24 * &Lt; Example 1 > + < Example 2 & 39.4 ± 4.93 32.3 ± 2.64 * * Example 1 + Example 2 is a mixture of the essential extract of Example 1 and the essential extract of Example 2.
* p < 0.05 by paired t-test

The results of the above Table 3 show that the essential oil extracts of the Examples lower the anxiety level and the anxiety index is lower when the essential oil extract of Example 1 and the essential oil extract of Example 2 are mixed.

&Lt; Experimental Example 3 > Animal experiment for antistress activity

Experimental animals were purchased from a 6 - week - old male Wistar rats (Shizuoka Laboratory Animal Center, JP). The animals were purified for one week before starting the experiment and then started using only healthy animals. The breeding environment was controlled by temperature: 22 ± 1 ℃, humidity: 50 ± 10%, illumination: 12hr / day.

Samples were mixed with drinking water and administered orally at a dose of 200 mg / kg once a day for 4 weeks. AIN-93G was fed to the basic diet.

Stress was induced by restraint stress, specifically, in a restraint cage (BioLink, Model DH-SC03) for 5 hours after 4 weeks of sample administration.

The experimental group consisted of 1 group of nonstress inducing group (normal group, n = 10) and 4 groups of stress inducing group (1 sample non - administration group and 2 group sample administration group, n = 10).

Serum concentrations of cortisol and creatine phosphokinase were measured to assess anti-stress activity.

The results are shown in Table 4 below.

Serum concentration of cortisol (/ / ㎗) division Serum concentration of cortisol (/ / ㎗) Nonstress-inducing group 6.32 ± 3.14 Stress Control 36.27 ± 5.46 Stress Experimental Group 1 (Example 1) 29.32 + - 6.24 Stress Experimental Group 2 (Example 2) 26.52 + - 4.72 Stress Experiment Group 3 (a mixture of the essential extract of Example 1 and the essential extract of Example 2) 23.62 ± 5.31

The results of the above Table 4 show that when the essential oil extract of the Example is orally administered to an experimental animal, the concentration of cortisol in the blood is lowered. Also in this case, the blood concentration of cortisol was lowered to the highest level when the same amount of the essential oil extract of Example 1 and the essential oil extract of Example 2 was orally administered.

Claims (6)

(i) a fragrance composition comprising at least one selected from the group consisting of essential oil extracts of Seokchangpo root, Seokchangpo stem oil extract, essential oil extracts of Seokchangpo root and stem mixture, and (ii) immature and essential oil extracts of Visa tree.
The method according to claim 1,
Wherein the perfume composition has a psycho-sedative effect.
9. A fragrance product comprising the fragrance composition of claim 1 or 2.
The method of claim 3,
Wherein the product is a fragrance product characterized by being a fragrance, a cosmetic, a soap, a hair treatment composition, a body care composition, a bath agent, a human body cleaner, a kitchen dishwasher, a disinfectant cleaner, a shower foam, a wet tissue, .
(i) an anti-stress food composition comprising at least one selected from the group consisting of essential oil extracts of Seokchangpo root, Seokjangpo stem oil extract, essential oil extracts of Seokchangpo root and stem mixture, and (ii) non-visa wood extract and essential oil extract.
6. The method of claim 5,
Wherein the composition is food or medicine.