KR101492854B1 - Natural Fragrance Composition Having Functionalities Using an Essential Oil Extract of Torreya nucifera - Google Patents

Abstract

The present invention discloses a functional natural perfume composition using a visa tree essential oil extract. Specifically, the present invention discloses a functional natural perfume composition using an extract of Vijaya essential oil having antimicrobial activity and antistress activity as an active ingredient.

Description

TECHNICAL FIELD The present invention relates to a functional natural perfume composition using a visa tree essential oil extract,

The present invention relates to a functional natural perfume composition using a visa tree essential oil extract.

As the economy develops and the standard of living improves, the demand for the appearance is increasing, and the demand for products such as cosmetics and fragrance to increase the appearance is also increasing. Perfume is a product made by blending a certain amount of perfume into alcohol and the like, and is used by being sprinkled on a part of the body or clothing to emit a fragrance.

Perfume, which is the main ingredient of perfume, can be classified into natural fragrance and chemical synthetic fragrance. Most perfume products currently use chemical synthetic fragrance. However, the chemical synthetic perfume does not give a continuous affinity to the user, and the additives used for long-lasting flavor may cause side effects such as allergic reaction, and recently, interest in perfume using natural essential oils obtained from plants is increasing.

Furthermore, recent trends in the consumption of perfume products are not limited to divergence of simple fragrance, but also products having additional functionality. Therefore, research on plant essential oils having functionalities and having excellent fragrances is actively conducted.

Meanwhile, Visa (榧子, Torreya nucifera ) is an evergreen coniferous tree belonging to Taxaceae and is distributed worldwide only in our country and Japan. Visa trees are used for edible, ornamental, industrial, and medicinal purposes. Seeds are eaten or squeezed out of oil.

In the oriental medicine and the private sector, the fruits are used for medicines such as insect repellence, hair growth, dry land, landscape, and intramuscular injection, and timber is used for building materials, machinery materials, ship lumber, etc. (Kim Taejung, 1996; Jang Changsoo, Asian herbal medicine book, p23, Book publication Kyungwon, 1997).

Separation and reported components of leaves and seeds of visa trees include sesquiterpenoids (Sakai, T. et al., Bull. Chem. Soc. Japan, 38: 381, 1965), labdane series And abietane-family diterpenoids (Sayama, Y. et al, Agric. Bio. Chem., 35: 1068, 1971; Harrison, L. and Asakawa, Y., Phytochemistry, 26: 1211, 1987 ), Flavonoids (Kariyone, T. and Sawaka, T., Ykugaku Zasshi, 78: 1010, 1958).

The present invention relates to the use of L-Limonene as an essential ingredient and a flavor such as essential oil extract and oil extract of Torreya nucifera having a function such as antistress activity .

It is an object of the present invention to provide a functional perfume composition using non-essential oil essential oil extract.

Other objects and specific objects of the present invention will be described below.

The inventors of the present invention have found that the extract of Viza japonica, essential oil extract and non-essential oil extract have an antibacterial activity against food harmful microorganisms, skin flora and / or acne bacteria, It was also confirmed that the fragrance was similar to the orange flavor. Particularly, the inventors of the present invention found that the essential oil extracts contained L-limonene as a main component as a result of the analysis of the essential oil extracts of Viza japonica, essential oils and visa trees, Limonene component because of the similar aroma to the L-limonene component.

The present invention has been completed on the basis of these experimental results. In one aspect, the present invention can be grasped as a functional perfume composition comprising an immature visa tree and an essential oil extract or an essential oil extract as an active ingredient.

In the present specification, the term " visa wood imperfecta "means flesh and skin of the visa tree before harvest (normally, visa refers to visa), and specifically, , It can be said that the seed coat (before the harvest time means the seed coat contains the pulp and the peel) in terms of being composed of the seed coat. More specifically, considering that the ripening in the harvesting stage has the characteristic of splitting (splitting) of the seeds, it is said that the seeds of the seeds of the seeds of the seeds of Viza japonica .

In the present specification, the term "functional" means the antimicrobial activity and / or antisstress activity as determined in the following experimental examples among the physiological activities of Viza japonica and essential oil extract or V. japonica essential oil extract. Specifically, the antimicrobial activity of Staphylococcus aureus ( Staphylococcus aureus) aureus , Staphylococcus epidermidis ) and / or propionibacterium acne .

In the present specification, the term "essential oil extract" refers to an extract of an oil-phase obtained by immersing a perfume ingredient in a viscera tree stem or immature seed which is an object to be extracted. Examples of the essential oil extracts include water vapor distillation method, solvent extraction method using volatile solvent (benzene, hexane, etc.), leaching method (method of collecting leachate by scratching the extraction target), compression method, supercritical extraction method Method can be used.

The perfume composition of the present invention may contain, as an essential ingredient, the active ingredients of Viza japonica, essential oil extract or essential oil extract or visa tree essential oil extract in an amount of 0.00001% by weight to 99.9999% by weight, By weight, preferably 0.00001% by weight to 30.0% by weight, and more preferably 0.00001% by weight to 10.0% by weight.

The product form in which the perfume composition is embodied includes soaps, cosmetics, bath salts, and the like, and specifically includes a body lotion, a shampoo, a hair conditioner, a hair conditioner, a hair treatment agent, a perspiration inhibitor, a skin lotion, a skin cream, a deodorant, Spray, or fumigant), lipstick, lip balm, bath salts, and the like.

These products may contain various additives such as blood circulation promoters, antiinflammatory agents, moisturizers, astringents, inorganic salts, organic salts, oily components, surfactants, herbal medicines, coloring matters, fragrances, sulfur, sinter deposits, .

In particular, the perfume composition of the present invention is generally used as an external preparation for skin of cosmetic formulations such as a makeup product, a skin lotion, and a skin cream. In this case, the perfume composition may contain components commonly used in these cosmetic formulations.

Examples of such components include inorganic powders and organic powders (e.g., homopolymers, copolymers, and resins). Particularly, talc, sericite, mica, kaolin, silicone resin, nylon and the like are preferable as such powder because of their excellent feel. The average particle size of these powders is preferably from 0.1 to 15 탆, particularly from 1 to 10 탆. When considering the feeling of use, the powder may be added in an amount of 1 to 60% by weight, particularly 5 to 25% by weight, based on the total weight of the composition for external application for skin.

The external preparation for skin of the present invention may also contain an oily substance. Preferred oily substances from the viewpoint of feeling in use are avocado oil, sucky oil, turtle oil, corn oil, olive oil, wheat germ oil, soybean oil, jojoba oil, peanut oil, cocoa butter, lanolin, liquid paraffin,

Squalane, squalene, petrolatum, cholesteryl ester, and silicone oil. The preferred content range of such an oily substance is 0.1 to 20% by weight, particularly 1 to 10% by weight.

In addition, the external preparation for skin of the present invention may contain an alcohol. Preferred alcohols include ethyl alcohol, propylene glycol, 1,3-butylene glycol, glycerol, sorbitol and the like. The alcohols can be used alone or as a mixture of two or more. In terms of feeling on use, the alcohol may be added in an amount of 0.1 to 30% by weight, particularly 1 to 20% by weight, based on the total weight of the composition for external application for skin of the present invention.

The composition for external application for skin of the present invention may further contain other functional substances such as a moisturizer, a whitening agent, an ultraviolet absorber, a bactericide, a limiting agent and the like.

The composition for external application for skin of the present invention may be manufactured in any form and may be in the form of, for example, a lotion, an emulsion, a cream, a powder, a poultice, a pack, a massage, a gel, a paste and a spray.

In addition, the perfume composition of the present invention may preferably be used by being mixed with a bath agent in view of its active ingredient, Viza japonica, essential oil extract or essential oil extract, The immature and essential oil extract or essential oil extract may be contained in an amount of preferably 0.00001 to 10.0% by weight, more preferably 0.0001 to 1.0% by weight, based on the total weight of the bath agent.

When the perfume composition of the present invention is incorporated in a bath agent, the bath agent may be added to bath water at a concentration of 0.015 to 15 ppm.

The bath agent may contain an inorganic salt, an organic acid, an oily component and the like in addition to the essential ingredient of the viscera and the essential oil extract or the visa tree essential oil extract, which are effective ingredients of the perfume composition of the present invention.

Examples of inorganic salts include sodium chloride, sodium hydrogen carbonate, sodium carbonate, borax, sodium sulfate, sodium sulfide, sodium sesquicarbonate, sodium nitrate, sodium thiosulfate, sodium polyphosphate, sodium phosphate, calcium oxide, magnesium oxide, calcium carbonate, magnesium carbonate, Potassium, and the like, which may be used alone or as a mixture of two or more. These inorganic salts may be added to the bathing agent in an amount of 5% by weight or more, preferably 10% by weight or more, based on the total weight of the bathing agent.

Examples of the organic acid include succinic acid, fumaric acid, malic acid, tartaric acid, citric acid, benzoic acid, etc. These may be used alone or in a mixture of two or more.

These organic acids may be added to the bathing agent in the range of 0.1 to 50% by weight based on the total weight of the bathing agent.

Examples of the oily component include wax, hydrocarbon, higher fatty acid, higher alcohol, ester, silicone oil and the like.

The bath salts may also contain other ingredients commonly used in the art. These components include (a) inorganic acids such as boric acid, meta silicic acid, and silicic anhydride; (b) herbal medicine powders such as fenugreek, bank, ginger, citrus peel, ginger root, peppermint, ginseng and oats; (c) (D) vitamins such as vitamin A, vitamin C, vitamin D and vitamin E, (e) sulfur, mica powder, white clay powder, Loess powder, rice bran carbide, fungicide, preservative and the like.

Such a bathing agent may be prepared by any desired means such as granules, tablets, liquids, powders, and the like.

In another aspect, the present invention relates to a composition for external application to skin for antibacterial use against Propionibacterium acne , an acne bacterium.

The following experimental example shows that the essential oil extract of visa tree has high antimicrobial activity against Propionibacterium acne which is an acne bacterium among various food harmful microorganisms and skin disease microorganisms.

Accordingly, the antimicrobial skin external composition for Propionibacterium acne of the present invention is characterized by containing an essential oil extract of Vijayama japonica as an active ingredient.

The composition for external application for skin of the present invention may be manufactured as a cosmetic formulation as described above with respect to the composition of the present invention or may be mixed with a pharmaceutically acceptable carrier, excipient or the like so that the active ingredient is mixed with ointments, pastes, transdermal preparations, And can be manufactured into a drug formulation.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

In another aspect, the present invention relates to an anti-stress composition.

The following Examples and Experimental Examples show that the immature and viscous oil extracts of Viza japonica or V. japonica have antistress activity in both animal and sensory evaluation.

Therefore, the anti-stress composition of the present invention is characterized by containing an immature visa tree and an essential oil extract or a visa tree essential oil extract as an active ingredient.

The anti-stress composition of the present invention may be formulated as a body lotion, a shampoo, a hair conditioner, a hair conditioner, a hair treatment, an antiperspirant, a skin lotion, a skin cream, a deodorant, Or a fumigant), a lipstick, a lip balm, a bathing agent, and the like. In particular, it can be made into a cosmetic product such as a skin lotion, a perfume, a bathing agent and the like.

The anti-stress composition of the present invention may also be prepared from a food composition.

When the anti-stress composition of the present invention is prepared with a food composition, the form of the food is not limited to the following: tea, juice, carbonated beverage, beverage such as ionic beverage, milk processed, processed oil such as route required, gum, rice cake, , Foods such as cotton, powders, tablets, capsules, and the like.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

As described above, according to the present invention, a functional perfume composition having anti-stress activity and the like can be provided.

The functional perfume composition of the present invention can be commercialized as an external preparation for skin or a bathing agent of a cosmetic formulation.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Immature or visa-free branches from  Extraction of essential oil

<Example 1> Preparation of immature and viscous oil extracts of Visa tree

2 to 4 liters of water per 300 g of viscous wood pulp and crumb pulp is heated to 70 to 80 ° C for 4 to 8 hours while the flask is connected to a distiller, The solution was cooled through a cooling tube inside the distiller to obtain a liquid containing visa oil. The distiller used here is a device used for the purpose of solvent separation and the like, and has a function of mixing the sample and the solvent and liquefying the gas produced when the mixture is heated through a cooling tube inside the still.

The obtained solution was put into a separating funnel and separated into a water layer and an oil layer, and then the separated oil layer was filtered to finally produce an oil extract.

Example 2 Extraction of Essential Oil from Visa Tree Branches

The essential oil extract of Viza japonica was prepared in the same manner as in <Example 1>.

< Experimental Example > Confirmation of antimicrobial activity, Antistress  Activity identification, flavor sensory evaluation and composition analysis

<Experimental Example 1> Confirmation of antibacterial activity

<Experimental Example 1-1> The strain

A total of 9 strains were used as food - harmful microorganisms and skin disease microorganisms. Food-harmful microorganisms were purchased from Difco (USA) as Tryptic soy agar (TSA), Tryptic Soy Broth (TSB), Nutrient Agar (NA) and Nutrient Broth (NB) Respectively.

Skin disease microorganisms are classified into three strains commonly found in skin as shown in [Table 2] and Staphylococcus epidermidis and Propionibacterium acne were selected and used. Propionibacterium acne was cultured in anaerobic Jar (Oxoid, USA) with anaerobic bacteria.

The culture media of these strains were purchased from Difco (USA) for Tryptic soy agar (TSA) and from Nissui (Japan) for GAM Broth.

<Experimental Example 1-2> Evaluation of antibacterial activity

Antimicrobial activity was measured by Disc diffusion assay method. Each strain was inoculated into 10 mL of liquid medium and subcultured three times for 18 hours at 37 ° C. The strain was used as a test strain for antimicrobial activity. The concentration of each test strain was adjusted to 10 ⁶ - 10 ⁸ CFU / mL with 0.5 McFarland Plate culture media were prepared by pour-plate method. Each sample was prepared at a concentration of 50 mg / L, slowly absorbed into paper discs (Whatman No. 5, 8 mm) by concentration, and the solvent was completely evaporated (please confirm). The antimicrobial activity was measured by measuring the size of the clear zone (mm) produced around the disc after the disc was adhered to the surface of the test plate and incubated at the optimum temperature of 37 ° C.

The results are shown in [Table 3] to [Table 6] below.

The antimicrobial activity against food harmful microorganisms was similar to that of Example 1 except that the viscous wood extract and essential oil extract of Example 1 and the visa tree essential oil extract of Example 2 showed similar activities, The visa tree essential oil extract of Example 2 showed a slightly higher activity than the immature and viscous oil extract of Example 1, and in particular, the visa tree essential oil extract of Example 2 exhibited an acne bacterium And showed high antibacterial activity against Propionibacterium acne .

<Experimental Example 2> anti-stress effect OK

<1> Experimental method

Experimental animals were purchased from a 6 - week - old male Wistar rats (Shizuoka Laboratory Animal Center, JP). The animals were purified for one week before starting the experiment and then started using only healthy animals. The breeding environment was controlled by temperature: 22 ± 1 ℃, humidity: 50 ± 10%, illumination: 12hr / day.

Samples were mixed with drinking water and administered orally at a dose of 200 mg / kg once a day for 4 weeks. AIN-93G was fed to the basic diet.

Stress was induced by restraint stress, specifically, in a restraint cage (BioLink, Model DH-SC03) for 5 hours after 4 weeks of sample administration.

The experimental group consisted of one non - stress - induced group (normal group, n = 10) and three stress - induced groups (n = 10).

Serum concentrations of cortisol, glucousa, lactic acid and creatine phosphokinase were measured to assess anti-stress activity.

<2> Experimental results

The results for the blood concentration of each marker against antistress activity are shown in [Table 7] to [Table 10] below.

Serum concentration of cortisol (/ / ㎗) division Serum concentration of cortisol (/ / ㎗) Nonstress-inducing group 6.23 + - 2.52 Stress Control 36.27 ± 5.46 Stress test group 1 (crude extract group of Example 1) 23.67 ± 5.26 Stress test group 2 (crude extract group of Example 2) 28.43 + - 4.39

Glucose concentration in blood (/ / ㎗) division Glucose concentration in blood (/ / ㎗) Nonstress-inducing group 102.48 + - 8.20 Stress Control 148.24 + 13.53 Stress test group 1 (extract group of Example 1) 122.16 ± 14.10 Stress test group 2 (extract group of Example 2) 136.38 + - 9.32

Serum concentration of lactate (㎍ / ㎗) division Serum concentration of lactate (㎍ / ㎗) Nonstress-inducing group 4.23 + - 0.85 Stress Control 39.21 + - 7.30 Stress test group 1 (crude extract group of Example 1) 27.64 ± 5.92 Stress test group 2 (crude extract group of Example 2) 34.75 ± 8.32

Serum concentration of creatine phosphokinase (/ / ㎗) division Serum concentration of creatine phosphokinase (/ / ㎗) Nonstress-inducing group 42.64 + - 0.62 Stress Control 95.27 ± 12.63 Stress test group 1 (crude extract group of Example 1) 77.93 + - 11.20 Stress test group 2 (crude extract group of Example 2) 82.46 + - 8.28

As shown in the above [Table 7] to [Table 10], the stress-induced group showed a marked increase in the concentration of stress-related blood marker than the non-stressed group, When the immature and essential oil extracts of Example 2 and the visa tree essential oil extract of Example 2 were administered, the concentration of these blood marker factors decreased. In particular, the immature and viscous oil extracts of Viza japonica var. And the activity of the extract was higher than that of the essential oil extract of Example 2.

&Lt; Experimental Example 3 >

The sensory evaluation of the essential oil extract of Example 1 and the essential oil extract of Example 2 were divided into two groups and evaluated for the satisfaction of horses.

2 ml of each essential oil extract of each of the above Examples was placed in a glass bottle of 10 ml and presented to a panel that understood the purpose of the sensory evaluation at room temperature. The satisfaction of each sample was selected as one of three items (good, normal, and poor) .

The results are shown in Table 11 below.

Sensory Evaluation Results division good usually Poor The essential oil extract of Example 1 16 3 One The essential oil extract of Example 2 14 4 2

The results of the above Table 11 were mainly evaluated as good evaluation, and the characteristic of fragrance was similar to orange flavor.

&Lt; Experimental Example 4 >

In order to analyze the components of the immature and viscous essential oils obtained in Examples 1 and 2, quantitative analysis was carried out using gas chromatography as follows.

The analytical equipment used was GC-MSD (Model Agilent 7890A), HP-5MS column was used, and the oven temperature was increased by 3 ° C at an initial temperature of 40 ° C for a final temperature of 250 ° C. The inlet temperature was 270 ℃ and helium was used as carrier gas with flow rate and split ratio of 1 ml / min and 20: 1. Each peak analyzed was compared with the search library (W9N08.L, Wiley) and the components were analyzed and quantitated.

The results are shown in [Table 12] and [Table 13] below for essential oil of Viza japonica and essential oils of essential oils and visas, respectively.

Analysis of viscous wood immature and essential oil components Name of sample Visa wood immature and essential oils ingredient NO. RT (min) a Area (%) b Component Quality (%)





main ingredient
One 9.55 17.72 α-Pinene 97 2 11.96 3.15 β-Mycrene 96 3 13.88 58.66 L-Limonene 98 4 16.33 1.7 alpha-terpenolene 98 5 31.46 1.11 Caryophyllene 99 6 34.02 2.11 1,6-Cyclodecadiene 98 7 35.55 3.98 Cardinal-1,10-diene 99 8 40.49 1.44 γ-Muurolene 98



Trace component One 10.18 0.31 Camphene 98 2 11.18 0.63 Sabinene 97 3 11.37 0.58 β-Pinene 97 4 13.15 0.18 α-Terpinene 97 5 15.07 0.25 γ-Terpinene 97 6 31.89 0.41 Germacrene-D 98 7 34.44 0.98 epi-Bicyclosesquiphellandrene 95 8 34.6 0.55 Germacrene-B 97 9 34.75 0.61 α-Muurolene 99 10 39.77 0.67 Cardine-1,4-diene 97 * A total of 18 analytes were analyzed based on 95% or more of the 25 analytical components. Only 1% of the analytes were classified into major components and minor components.
* a. Time at which the component was detected
b. The content of the component in the sample

Visa tree branch essential oil component analysis result Name of sample Visa Tree Branch Essential Oil ingredient NO. RT (min) a Area (%) b Component Quality (%)





main ingredient
One 9.54 17.1 α-Pinene 97 2 11.93 1.48 β-Mycrene 96 3 12.79 13.69 δ-3-carene 96 4 13.81 29.47 L-Limonene 98 5 16.32 1.94 alpha-terpenolene 98 6 28.37 1.13 α-cubebene 95 7 32.59 1.34 β-Farnesene 96 8 33.78 1.07 alpha-amorphene 99 9 34.01 1.64 Germacrene-D 98 10 34.43 1.14 Epi-bicyclosesquiphellandrene 95 11 35.55 7.05 δ-Cadinene 98 12 39.77 1.8 Cadina-1,4-diene 96 13 40.5 4.11 γ-Muurolene 98




Trace component
One 10.1 0.8 α-Fenchene 97 One 10.17 0.23 Camphene 98 3 11.36 0.76 β-Pinene 97 4 13.14 0.18 α-Terpinene 97 5 15.05 0.19 γ-Terpinene 96 6 16.54 0.13 p-Cymenene 97 7 21.64 0.26 Estragole 98 8 25.67 0.87 Anisole 98 9 31.44 0.37 Caryophyllene 99 10 34.59 0.61 Germacrene-B 97 11 35.66 0.36 cis-Calamenene 98 12 40.8 0.98 Cedrelanol 97 * A total of 25 analytes were analyzed based on 95% or more of the 47 analytical components. Only 1% of the analytes were classified into major components and trace components.
* a. Time at which the component was detected
b. The content of the component in the sample

As a result of the analysis of the components of the above [Table 12] and [Table 13], 8 main components and 10 minor components of Viza japonica immature and essential oil extracts were analyzed, and the main component was analyzed as L-Limonene (58.66%), In the case of essential oil extracts of tree branches, 13 major components, 12 minor components and the main component were analyzed as L-Limonene (29.75%).

In view of the fact that the main ingredient is L-Limonene, the antistress activity, antimicrobial activity, and sensory evaluation result as an antioxidant activity are presumed to be due to L-Limonene.

Claims (10)

A composition for anti-stress comprising an immature visa and essential oil extract as an active ingredient.
The method according to claim 1,
Wherein the composition is a perfume composition.
The method according to claim 1,
Wherein the composition is a perfume composition and the perfume composition is a spray formulation.
3. The method of claim 2,
Wherein the composition is an external preparation for skin.
The method according to claim 1,
Wherein said composition is a bath composition.
The method according to claim 1,
Wherein the composition is a perfume composition and the perfume composition is a fumigant formulation.
delete delete delete The method according to claim 1,
Wherein said composition is a food composition.