KR20140115424A - Essential Composition with Carboxy Generating Substance for Improving Blood Flow and Treating Acne and Method for Producing the Same - Google Patents

Abstract

The present invention relates to a cypress fermented essential composition including a carboxy-generating substance for improving blood flow and treating acne, and a method for manufacturing the same. More specifically, the present invention relates to a cypress fermented essential composition including a carboxy-generating substance for improving blood flow and treating acne which is manufactured by fermenting an active ingredient extracted from the cypress leaf. The method for manufacturing the composition, according to the present invention, comprises the steps of: preparing the cypress leaves; hot water-extracting, ethanol extracting, microwave treating, or supercritical extracting the prepared cypress leaves to concentrate under reduced pressure; and fermenting the cypress extract concentrated under reduced pressure in Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum, or Aspergillus niger.

Description

FIELD OF THE INVENTION The present invention relates to a composition for improving the blood flow and a method for producing the same, which comprises a carboxy generating material for treating acne, and a method for producing the composition.

The present invention relates to a basic composition for the production of a white flour fermentation comprising a carboxy generating material for improving blood flow and treating acne, and to a method for producing the same. Specifically, the present invention is useful for improving blood flow and treating acne produced by fermenting an effective ingredient A carbohydrate-generating substance, and a method for producing the same.

The blood flow due to the slope of the blood pressure in the vascular system is called blood flow, and if a blood flow obstacle occurs, various kinds of diseases may occur because the supply of nutrients and the discharge of waste required in each part of the body are not normally performed. Blood flow disorders can occur for reasons such as decreased fluidity of the blood, reduced inner diameter of the blood vessel due to an increase in cholesterol, or increased coagulation of platelets. A variety of methods for improving such blood flow disorders have been proposed and Patent Disclosure No. 2011-0019411 " Strain for improving blood flow and a functional plant composition containing the same " includes Lactobacillus palapentaarum, Wyselasibaria, Discloses a set of mature seeds for improving blood flow including steroids, lactococcus lactis, and saccharomyces cerevisiae.

On the other hand, acne, which is an inflammatory disease, can be caused by androgen, skin keratin production, sebaceous germ interaction, and is known to be caused, for example, by the secretion of lipopolysaccharide of propionibacterium acnes . Background Art [0002] Prior art related to the treatment of acne is Patent Publication No. 2009-0127523 entitled " Atopic and acne treatment composition ". The above-mentioned prior art is a composition for treating acne based on a hot-water extract of a mixture of Zysei, Baekjoo, Sukjyuwang, Astragalus, Mulberry, Golden, Waxy, Licorice, Peony, .

Carboxytherapy refers to a treatment method for dissolving fat by injecting a liquid gas into the body for the purpose of decomposing body fat and reducing cellulite so that the surroundings are released into blood vessels or lymphatic vessels. Carboxytherapy is a method of inducing the reduction of intracellular fluid, extracellular fluid, protein, mineral, body fat, body water, dietary fat and body weight by the injection of carbon dioxide into the human body. Carboxytherapy is a non-surgical cosmetic restorative treatment and can be done by injecting carbon dioxide on the surface of the skin by microdermabrasion or injection for removal of wrinkles, scars, fats or other skin conditions.

As a prior art related to a carbon dioxide-containing skin treatment composition, Patent Document No. 2001-0031864 entitled " Carbon dioxide-containing viscous cosmetic composition " The prior art is capable of treating an external antihistamine or an antiallergic agent, a non-steroidal anti-inflammatory agent or a steroid, and at the same time, a thickener 1 having a wound treatment promotion, skin beauty, partial obesity- Discloses a carbon dioxide-containing viscous composition containing bubbling carbon dioxide in a viscous composition containing more than two species. The thickener may be selected from the group consisting of natural polymers, semisynthetic polymers, and inorganic materials. For example, natural polymers may be selected from the group consisting of gum arabic, carrageenan, galactan, agar, corn gum, guar gum, tragacanth gum, Group consisting of starch, lactose, starch, rice starch, corn starch, potato starch, cardron, xanthan gum, succinoglucan, dextran, hyaluronic acid, pullulan, albumin, casein, collagen, gelatin and fibroin Can be selected.

This prior art or known acne treatment or carboxy potency has the disadvantage that the composition is intended to have an effect on one symptom. It may have a disadvantage in that a separate mechanism is required or a long time is required to express the effect in the human body. When a composition for treatment is manufactured, for example, in the form of a cosmetic product and applied to a human body, it is advantageous to introduce the effect into a predetermined region of the human body within a predetermined time. In addition, it is advantageous to have multiple effects simultaneously in one composition component, especially in the case of cosmetic compositions.

The present invention has been made to solve the problems of the prior art and has the following purpose.

It is an object of the present invention to provide a flocky fermentation-based composition comprising a carboxy generating material for blood flow improvement and acne treatment.

Another object of the present invention is to provide a method for preparing a monofilament fermentation-based composition comprising a carboxy generating material for improving blood flow and treating acne.

According to a preferred embodiment of the present invention, the monofilament fermentation base composition comprises an extract obtained by liquid extraction of the flat leaves, or a fermentation product obtained by fermenting dry-milled flat leaves with Lactobacillus.

According to another preferred embodiment of the present invention, it further comprises an extract of a mash or kelp.

According to yet another preferred embodiment of the present invention, a method of making a monofilamented fermentation base composition comprises the steps of: preparing a leathery leaf; Concentrating the prepared white cotton leaves by hot water extraction, ethanol extraction, microwave treatment or supercritical extraction; And a step of fermenting the depressurized and concentrated polysaccharide extract in Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum or Arpergillus niger.

According to another preferred embodiment of the present invention, the step of extracting or hot-water extraction the mesophyll or sea tangle with an acid-base is further included.

According to still another preferred embodiment of the present invention, a method for producing a monofilament fermentation base composition comprises drying and crushing the flat leaves; And the pulverized soft white leaves are fermented in an MRS medium for 2 to 4 weeks using Lactobacillus.

The composition according to the present invention has an advantage of being added to cosmetics for treating acne or other functional agents so that excellent efficacy can be exhibited. In addition, the composition according to the present invention has an advantage that it can be added to a cosmetic or functional agent without the need of a separate treatment process, so that the same effect as that of carboxytherapy can be obtained. The composition according to the present invention has an advantage that it can be used as a raw material for a cosmetic composition having various functions because it has stability in various environmental conditions and can maintain its effect for a predetermined time without side effects. In addition, the composition according to the present invention is advantageous in that it is environmentally friendly in the manufacturing process without involving adverse effects due to the use of the human body as a natural composition.

FIG. 1 schematically shows an embodiment of a process for producing a composition according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the embodiments shown in the accompanying drawings, but the present invention is not limited thereto. In the following description, components having the same reference numerals in different drawings have similar functions, so that they will not be described repeatedly unless necessary for an understanding of the invention, and the known components will be briefly described or omitted. However, It should not be understood as being excluded from the embodiment of Fig.

The composition according to the present invention may further comprise an extract obtained by extracting a flat leaf from a liquid phase or a fermented product obtained by fermenting a dried wheat flour with a lactobacillus, and further, an extract of a mash or kelp.

Hereinafter, a method for producing a composition according to the present invention will be described in detail.

FIG. 1 schematically shows an embodiment of a process for producing a composition according to the present invention.

Referring to FIG. 1, the composition according to the present invention comprises a step (S11) of preparing a flat leaf, a step (S12) of extracting an active ingredient from the prepared cotton leaf, a step (S13) (S14) of extracting an effective ingredient from marine algae such as kelp, and a step (S15) of preparing a composition containing leachate fermentation extract and algae extract.

Pruning is a plant belonging to the evergreen coniferous tree family, which is resistant to cold tolerance and salt tolerance but resistant to environmental tolerance. It is known that it contains antimicrobial substances called phytoncit, and it has skin allergy-improving skin diseases, antibacterial, anti-stress, deodorizing and purifying function and immunity function. For the preparation of the composition according to the present invention, soft white leaves may be prepared and the prepared soft white leaves may be washed and dried. The active ingredient can be extracted from the prepared white cotton leaf (S12).

The active ingredient can be obtained using hot water extraction, ethanol extraction, microwave extraction or supercritical extraction. The hot water extraction can be carried out by, for example, making the distilled water at 80 to 100 캜 and dipping the soft white leaves for 2 to 6 hours. The extract can then be filtered and concentrated under reduced pressure. The ethanol extraction can be carried out by immersing the filamentous leaves in ethanol and incubating at 30 to 45 DEG C for 2 to 5 days, after which the extracted active ingredients are filtered and reduced in pressure and concentrated. Microwave extraction can be carried out by immersing the flat leaves in distilled water, microwaving for 10 to 50 minutes, filtering the extract, and concentrating under reduced pressure. And the supercritical extraction is carried out with carbon dioxide or pentane at a temperature of 300 to 400 bar at a temperature of 70 to 90 DEG C for 2 to 6 hours and an extraction efficiency of 0.2 to 0.5 wt% of the sample weight.

When the active ingredient is extracted from the flat leaf, the extract may be fermented (S13). For fermentation, bacteria such as, for example, Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum or Aspergillus niger may be used. have. The fermentation can be carried out at a temperature of 20 to 35 ° C for 2 to 6 days, and the fermentation broth can be further centrifuged at 10,000 to 20,000 rpm for 10 to 30 minutes in a centrifuge. Thereafter, the supernatant separated by centrifugation may be concentrated.

The flourishing fermentation extract according to the present invention can be obtained by solid fermentation including liquid fermentation as described above. Specifically, it is washed and dried, and the dried laver leaves are finely crushed to form a powder. Thereafter, it may be fermented in the MRS medium with lactobacillus bacteria or preferably with lactobacillus plantarum for 2 to 6 weeks. The fermentation can be carried out, for example, at 20 to 35 ° C.

When the fermented monofilament extract is obtained (S13), algae extract can be prepared (S14). Seaweeds can be, for example, marine or kelp and can be hydrothermal or acid-base extracted. For hydrothermal treatment of seaweed or kelp, desalting treatment may be performed first. The desalination treatment can be carried out by cutting the seaweed or sea tangle into a length of, for example, 10 to 30 cm, immersing it in distilled water, and applying ultrasonic waves of 5 to 30 kHz with stirring. It may be desalted by repeating the same process for 3 to 6 times while applying ultrasonic waves for 20 to 30 minutes and supplementing the distilled water. The seaweed or kelp extract can then be obtained by soaking the demineralised seeds or sea tangle in ethanol at a temperature of 30 to 50 DEG C for 10 to 30 hours. Thereafter, the filtrate is concentrated under reduced pressure to obtain the final extract. In order to acid-base extract the seaweed or kelp, a cauliflower or sea tangle cut into a length of 10 to 30 cm with an aqueous solution of sodium hydroxide or potassium hydroxide is put into an aqueous solution of sodium hydroxide or potassium hydroxide and stirred for 20 to 40 minutes And then allowed to stand for 10 to 30 hours thereafter. Thereafter, it is washed using a distilled water solution, and the mixture is put into vinegar or a similar weak acidic aqueous solution, and the mixture is stirred for 20 to 40 minutes and then allowed to stand for 10 to 30 hours. After washing with distilled water, it is subjected to hot water extraction using distilled water or ethanol, and concentrated under reduced pressure to obtain seaweed extract. Seaweed extracts can be obtained in a variety of ways and the present invention is not limited to the embodiments shown.

The seaweed extract can be added to the obtained white flaky fermentation extract to provide a floury fermentation-based composition containing a carboxy-generating material for improving blood flow and treating acne, and can be used as a raw material for cosmetics or other functional preparations. The seaweed extract may be 3 to 30 wt% based on the total composition.

The composition of the present invention has been shown to have effects on the treatment of acne, atopic dermatitis or other skin troubles. In addition, it has been shown to have the effect of eliminating skin cosmetics, wrinkles, scars, fats or other skin at least equal to or more than carboxytherapy. This effect can be obtained by the perennial fermentation extract itself, and the effect is further increased by addition of the mash or seaweed extract.

Hereinafter, an embodiment of a method for producing a composition according to the present invention will be described.

Example

Step 1: Immersed in 100 g of white flour leaf in 500 g of distilled water at 90 to 95 캜 for 4 hours, followed by hot water extraction. The resulting filtrate was filtered and concentrated under reduced pressure to give 15 ml.

Step 2: The concentrated extract was fermented with Lactobacillus fermentarium at 25 to 30 DEG C for 4 days.

Step 3: The fermentation broth was centrifuged at 12,000 rpm for 20 minutes in a centrifuge to obtain a supernatant, which was concentrated under reduced pressure to about 1 to 5 vol% of the total volume.

Step 1: 100 g of white waxy leaves were incubated in 500 ml of ethanol at 35 to 40 占 폚 for 300 days, and the mixture was reduced in pressure and concentrated to 15 ml.

Step 2 and step 3 were carried out in the same manner as in Example 1, except that they were fermented with Lactobacillus brevis.

Step 1: 100 g of white waxy leaves were placed in 600 ml of distilled water, treated with microwave for 20 minutes, and then concentrated under reduced pressure to 15 ml.

Step 2 and step 3 were carried out in the same manner as in Example 1, except that they were fermented with Lactobacillus plantarum.

Step 1: 1.8 kg of white floret leaves were subjected to supercritical extraction using carbon dioxide at a pressure of 350 bar and a temperature of 80 ° C for 4 hours to obtain 5.1 g of an extract.

Step 2 and Step 3 were carried out in the same manner as in Example 1, except that they were fermented into Arpergillus niger.

Step 1: Pruning leaves were dried and made into powder form.

Step 2: Powdery leavened leaves were fermented with Lactobacillus plantarum at a temperature of 25 to 30 DEG C for 4 weeks in MRS medium.

To proceed with Examples 1 to 5 and to confirm the efficacy of the addition of the mash and seaweed extracts, the mash was subjected to hot water extraction with ethanol and the kelp was acid-base extracted.

The granular extract was added to the fermented extract of Example 5 in an amount of 5 wt% of the total weight.

The kelp extract was added to the fermented extract of Example 5 in an amount of 5 wt% of the total weight.

For the efficacy of the fabricated fermentation base composition, it was applied to blood flow improvement, acne treatment and carboxytherapy test. For the test, acne bacteria were first cultured.

Acne bacteria ( Propionibacterium acnes ) Culture

The acne bacteria were put into an anaerobic jar of a BHI (Brain Hear Infusion) medium and cultured at 25 to 30 ° C for 24 hours.

Efficacy evaluation

The efficacy was evaluated by bioassay using a paper disk in a medium suitable for each Example. Specifically, the composition of each Example was added to a liquid medium, and the bacteria were cultured to obtain OD (Optical Density ) Were measured to evaluate the degree of inhibition of the bacteria.

Test result

end. Efficacy for five common strains

Fig. 2A shows the results of the efficacy tests on five strains. In the results shown in Figs. 1 and 2, the antimicrobial test results of the examples and the antimicrobial test results of the control examples 3 and 4, respectively.

For the control, the extracts obtained by hot-water extraction of leek leaves were used as general bacteria such as Aspergillus niger, E. coli, Candida albican, Staphylococcus aureus and Pseudomonas auruginosa ) Were subjected to an antibacterial test. The control group showed some antimicrobial activity against Staphylococcus aureus.

Examples 1 to 5 showed antibacterial activity against the same bacterium, showing efficacy against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and especially against Staphylococcus aureus.

I. Acne bacteria  Efficacy

FIG. 2B shows the results of the efficacy tests of Examples 2, 3 and 4 against acne bacteria and the results of the efficacy tests of the control.

The test results of Examples 2, 3 and 4 were presented in the clockwise direction from below in FIG. 2B, and the test results at 3 o'clock showed the efficacy against the acne bacterium for the hydrolyzed water by the distilled water.

All. Test for efficacy of liquid fermentation and solid fermentation

The efficacy tests of the acne bacteria of Examples 1 and 5 were compared and the test results are shown in Fig. 2c. 2, and 3 show the boiling concentration, the reduced pressure concentration, and the efficacy of Example 5 for Example 1, respectively. Solid fermentation showed better efficacy in liquid fermentation.

la. Over time Acne bacteria  Inhibitory efficacy

Examples 1 and 5 were tested for their antimicrobial activity against time and presented in FIG. 2d.

The upper curve of FIG. 2 (d) shows the inhibitory effect of Example 1 over time and the lower curve shows the inhibitory effect of Example 5, respectively. It can be seen that in Example 5, the efficacy is maintained regardless of the passage of time.

hemp. Stability test

The activity stability was tested for Example 5 at 45 < 0 > C for 72 hours under harsh conditions and presented in Figure 2e. In Figure 2e, marked 1 indicates efficacy before the harsh condition and 2 indicates efficacy after the harsh condition. It can be seen that the efficacy is maintained after severe conditions.

bar. Stability test for light

Example 5 was exposed to light for 5 hours and 11 hours and tested for efficacy and the results are shown in Figure 2f. In Figures 2f, 1, 2 and 3 show efficacy before (0 h), 5 h and 11 h exposure, respectively. Indicating that efficacy remains after at least 11 hours of exposure.

four. Blood flow improvement and Carboxy therapy  Efficacy test

The prepared composition was administered to the calves and thighs of the human body three times a day for 20 days. After 10 days of treatment and 20 days after treatment, blood was collected to test blood flow improvement. Body fat, body water, fat mass and thigh circumference were measured for carboxytherapy efficacy. The subjects who were judged to be obese were selected as subjects to be tested, and the average of 20 subjects except 25 subjects who did not show significance level were averaged.

The test results are shown in the following table.

* Blood flow was collected from the calves and the passage time of saline was measured using a micro channel array flow analyzer.

* Blood flow improvement is the rate of increase in%.

* Body fat, lean body mass and perimeter size mean decreased amount, and body water means increase amount and expressed as% value respectively.

* Body fat, body water and fat mass were measured by bio-electrical impedance analysis and the peripheral size was measured directly.

The compositions according to the present invention show improved blood flow and a carboxy-generating material, respectively, which show that this effect is increased due to the addition of marigold and kelp extract.

The composition according to the present invention has an advantage of being added to cosmetics for treating acne or other functional agents so that excellent efficacy can be exhibited. In addition, the composition according to the present invention has an advantage that it can be added to a cosmetic or functional agent without the need of a separate treatment process, so that the same effect as that of carboxytherapy can be obtained. The composition according to the present invention has an advantage that it can be used as a raw material for a cosmetic composition having various functions because it has stability in various environmental conditions and can maintain its effect for a predetermined time without side effects. In addition, the composition according to the present invention is advantageous in that it is environmentally friendly in the manufacturing process without involving adverse effects due to the use of the human body as a natural composition.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention . The invention is not limited by these variations and modifications, but is limited only by the claims appended hereto.

S11: Cotton leaf preparation S12: Cotton protein extraction
S13: Flavor extract fermentation S14: Seaweed extract
S15: Composition manufacturing

Claims (5)

A composition for improving the blood flow and a carboxy generating material for acne treatment, comprising a fermented product obtained by fermentation of Lactobacillus fermented with an extract obtained by extracting the leathery leaves or a dried and pulverized soft leaved leaf. The flocked fermented basestock composition of claim 1, further comprising an extract of a mash or kelp. A method for producing a monofilament fermentation base composition,
Preparing a cotton bud;
Concentrating the prepared white cotton leaves by hot water extraction, ethanol extraction, microwave treatment or supercritical extraction; And
Comprising the step of fermenting the depressurized concentrated cottonseed extract in Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum or Arpergillus niger, and a carboxy generating material for improving blood flow and treating acne, A method for producing a monofilament fermentation basic composition. [4] The method of claim 3, further comprising the step of extracting or hot-water extracting the mash or sea tangle with an acid-base. Drying and crushing the flat leaf; And the pulverized soft white leaves are fermented in an MRS medium for 2 to 4 weeks using Lactobacillus.

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