JP2014058500A - Safflower fermentation liquid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel plant-derived cosmetic, a collagen production enhancer, a fibroblast proliferation agent, a keratinocite proliferation agent, an antioxidant, a tyrosinase activity inhibitor, and/or a humectant.SOLUTION: A cosmetic, a collagen production enhancer, a fibroblast proliferation agent, a keratinocite proliferation agent, an antioxidant, a tyrosinase activity inhibitor and/or a humectant contain a safflower extract fermented by yeast of Saccharomycetales. The yeast is preferably genus Pichia, particularly preferably Pichia kudriavzevii.

Description

  The present invention relates to a cosmetic containing a safflower fermented liquid, a collagen production promoter, a fibroblast proliferating agent, a keratinocyte proliferating agent, an antioxidant, a tyrosinase activity inhibitor and / or a humectant.

Many studies have been conducted on the skin aging phenomenon.
WO 2009/084742 pamphlet describes a female in her 70s with 0.01% yeast extract and 0.1% safflower extract cultured in a nutrient medium containing glycosaminoglycan and subjected to ultraviolet irradiation and hydrogen peroxide treatment. A method for growing fibroblasts derived from dermal dermis has been described.

International Publication No. 2009/084742 Pamphlet

  The present inventor has intensively studied to provide a novel plant-derived cosmetic, collagen production promoter, fibroblast proliferator, keratinocyte proliferator, antioxidant, tyrosinase activity inhibitor and / or moisturizer. went.

  As a result, a safflower fermented liquid containing a safflower (Carthamas tinctorius Linne (Compositae)) extract that has been fermented with Saccharomyces yeasts of the Ascomycota family, It has been found useful as a fibroblast proliferation agent, keratinocyte proliferation agent, antioxidant, tyrosinase activity inhibitor and / or humectant.

It is a figure which shows the collagen production promotion test result of this invention. It is a figure which shows the fibroblast proliferation test result of this invention. It is a figure which shows the keratinocyte proliferation test result of this invention. It is a figure which shows the moisture retention test result using the epidermis horny layer water content measuring apparatus of this invention. It is a figure which shows the moisture retention test result using the moisture meter of this invention.

  As safflowers extracted in the present invention, safflower flowers can be used. You may use the crude drug (kouka) which dried the flower. Red rice cakes may also be used.

  The safflower may be subjected to an extraction operation as it is, or extraction may be performed after processing such as chopping, drying, and pulverization. Extraction is performed by immersing in an extraction solvent. In order to increase the extraction efficiency, stirring may be performed, or homogenization may be performed in an extraction solvent. The extraction temperature and extraction time can be appropriately selected.

The solvent for extracting the safflower extract is not particularly limited. Water, physiological saline, phosphate buffer, phosphate buffered saline, alcohols (for example, monohydric alcohol or hydrated monohydric alcohol such as methanol, ethanol, propyl alcohol, isopropyl alcohol, or propylene glycol, dipropylene glycol) , 1,3-butylene glycol, glycerin and other polyhydric alcohols or hydrous polyhydric alcohols) can be used alone or in combination of two or more of them in an arbitrary mixing ratio. A solvent such as ethanol, which is easy to remove after extraction, may be used alone, or may be used alone or in a mixed solution of any mixing ratio with water.
A solvent may be added after extraction.

  The substance obtained by extracting the safflower extract may be immediately subjected to fermentation, or may be subjected to fermentation after filtration, centrifugation, dilution, concentration and the like.

The yeast of the order Saccharomyces used in the present invention is not particularly limited, and may be Saccharomycesaceae (Saccharomycesaceae). The Saccharomyces family used in the present invention is not particularly limited, but may be Pichia. The genus Pichia may be classified as Pichiaceae, and the Saccharomyces used in the present invention may be Pichiaceae. The genus Pichia used in the present invention is not particularly limited, and may be Pichia kudriavzevii.
Prior to using yeast for safflower fermentation, hydrogen peroxide treatment and UV irradiation are not required. When culturing before using yeast for safflower fermentation, a medium containing a special substance such as glycosaminoglycan is not required, and for example, yeast extract-malt extract agar (YM agar) can be used. Further, for example, a Sabouraud glucose agar medium used in a test for determining the number of cells in a microbial test of the Japanese Pharmacopoeia may be used.
Various methods are known for pure culture of yeast. For example, “Science of Bacteria” (published by Jiro Ota, published by Kensei Co., Ltd., first printed on September 20, 1982) pp. 32-33. The method of description is mentioned.

  The safflower fermentation conditions (temperature, pH, time, medium, additives, etc.) are not particularly limited. For example, the fermentation temperature can be appropriately selected within the range of 20 to 40 ° C., the initial fermentation pH of 3.0 to 7.0, the fermentation time of 6 to 168 hours, and the like. Various nutrients, acids for adjusting pH, alkalis, and the like may be appropriately added at the start of fermentation or during fermentation. In the fermentation medium, components that can serve as nutrient sources such as sugar, yeast extract, peptone, or animal or plant-derived components can be used, and these components can be used alone or in combination of two or more. A specific single species may be used as the yeast, but two or more different yeasts may be used in combination. Moreover, you may use microorganisms other than yeast, for example, lactic acid bacteria, for the mixed fermentation with yeast, the secondary fermentation after yeast fermentation, or continuous fermentation.

A substance obtained after fermentation (referred to as fermentation product, fermentation solution, culture product or culture solution, or fermentation stock solution or culture solution) may be used as it is, or may be further processed. Examples of the treatment include sterilization treatment, pH adjustment, purification (filtration, centrifugation, ion exchange resin, activated carbon column, dialysis membrane, concentration, distillation), dilution, and drying.
The solvent used for the dilution is not limited. Water, physiological saline, phosphate buffer, phosphate buffered saline, alcohols (for example, monohydric alcohol or hydrated monohydric alcohol such as methanol, ethanol, propyl alcohol, isopropyl alcohol, or propylene glycol, dipropylene glycol) , 1,3-butylene glycol, glycerin and other polyhydric alcohols or hydrous polyhydric alcohols) can be used alone or in combination of two or more of them in an arbitrary mixing ratio. A solvent such as ethanol, which is easy to remove after extraction, may be used alone, or may be used alone or in a mixed solution of any mixing ratio with water. It may be the same as or different from the solvent for extraction of safflower extract.
The sterilization method is not particularly limited. For example, it can be sterilized by heating. In that case, temperature and time are not particularly limited. Regarding temperature, it can select suitably, for example, 50-140 degreeC, 70-120 degreeC, 90-100 degreeC. Regarding the time, a second level, a minute level, a time / day level, and the like can be appropriately selected. At the second level, 2 to 60 seconds, 5 to 30 seconds, 10 to 20 seconds, and the like can be appropriately selected. At the minute level, 2 to 60 minutes, 5 to 30 minutes, 10 to 20 minutes, etc. can be selected as appropriate. At the time / day level, 2 to 60 hours, 5 to 30 hours, 10 to 20 hours, and the like can be appropriately selected. Sterilization with drugs or light such as ultraviolet rays is also possible. These may be used in combination.

  The fermentation broth can be used in liquid form, but it may be used after being concentrated and dried to form a paste or powder.

The safflower fermented liquid of the present invention can be used as a cosmetic, a collagen production promoter, a fibroblast proliferating agent, a keratinocyte proliferating agent, an antioxidant, a tyrosinase activity inhibitor or a moisturizing agent. Depending on the use, the formulation (content ratio of safflower fermented liquid and the type and content ratio of other compounding components) can be appropriately selected from conventional formulation examples according to the technical common knowledge of those skilled in the art. For example, the safflower fermented liquid of the present invention can be used in the formulation of a conventional skin external preparation. Examples of the external preparation for skin include a patch, lotion, emulsion, cream, ointment and the like.
You may call what is obtained by mix | blending another compounding ingredient with a fermentation undiluted | stock fermented material, a fermented liquid, a culture, or a culture solution.

  You may mix | blend another compounding component in any step which manufactures a safflower fermented liquor.

  Examples of other compounding components include the following components.

(1) Various oils and fats Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cacao oil, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber nut oil, safflower oil , Shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, owl, palm Oil, beef tallow, pork tallow, squalene, squalane, pristane or hydrogenated products of these oils (hardened oil, etc.).

(2) Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.

(3) Mineral oils Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid Natural fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, phenoxyethanol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol .

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.

(7) Esters isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gums, sugars or water-soluble polymer compounds Arabic gum, benzoin gum, danmar gum, guaiac gum, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, glucose, mannose, lactose, fructose, sucrose Or an ester thereof, trehalose or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, and an hydroxy (C2 to C4) chitin or chitosan to which an alkylene (C2 to C4) oxide such as ethylene oxide is added. Low molecular chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, Cellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinylmethylether, polyvinylpyrrolidone, polyvinylmethacrylate, polyacrylate, polyethylene oxide, Polyalkylene oxide such as polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, polyethyleneimine and the like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric Surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants ( Natural surfactants, protein hydrolyzate derivatives, polymer surfactants, surfactants containing titanium and silicon, Carbon-based surface active agent) and the like.

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C acid or derivatives thereof, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2) Menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine and the like, and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal materials These are processed in a conventional manner according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, fermentation, purification) , Compression, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc., arbitrarily selected and combined), arbitrarily selected from various materials You can use it.

Specific plants (herbal medicines) used as raw materials include, for example, almonds (Peach), Ai (Aiba), Aokazura (Seifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Rafuji , Aotenma, acacia, red ginger, red spruce “fruit”, red clover, red grape, red ginseng (logwood), red wrinkle (red buds), red crested or red crested or reddish (root), red crested or reddish (ground yellow), Agi (Aoi), Aki larch, Akinire (crusted skin), Aguay Guassou, Aguahe (Omiten coconut palm), Akebei (Kitsu), Asa (Asakojin), Marubaasagao or morning glory (Chicken), Hydrangea (hydrangea), Ashitaba (Tomorrow), Azuki (red bean), Asunaro, Acerola, Asen Ku (Asen Yaku), Athira (Shokuyo Kanna), Anise, Abiu, Abiurana, Brassica, Abemaki "Fruits", Avocado, Ama, Achacha (Amacha), Achacharu, Amadokoro (Tamatake), Amana (Kojiko), Amaranthus (Ayumu, Hagatetou, Higegateto, Senninkoku, Sugimorigateto, Hosoaogeito, Aogatetou, Haribiyu, Aozulan, Amaranthus hippochondriacus), Amigasayuri or Baimo (shellfish mother), Aristolomeria (Yurizuisen), Arcana (Arrouna) , Altea, Arnica, Alpinia or Katsudai (Suzukuku), Aloe (蘆薈), Aloe Vera, Angelica, Apricot or Honanzu (Anzujin), Ansokkou (benzoic incense), Yellow support, Igakozorina (Gall head) , Ikarisou or Yatsuma Taikiso (Japanese Yakukaku), rush (Ginseng grass), Japanese knotweed (Tiger root), Yew (first place), Strawberry, Fig (no flower "fruit, leaf"), Ichihatsu (first), Ichibibi (winter lion) ), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo "seed, leaves"), Itohimehagi (dianthus), Carob, Tuna (Telexi), Inubiyu (Honagainubiyu), Rice "Seeds, seed coat", Inondo "seed", Ibukizasou, Nettle, Ylang Ylang, Iwa Tobacco (Iwabuchi), Iwahiba or Iwamatsu (Makimochi), common bean, oolong tea, fennel (mud), wheat (Chibusanoki), Ukiyara (three-ridges), Uguisukagura "fruit", Japanese fennel, turmeric ( Ugly), Duckweed, Usubasaicin or Keirinsaishin or Oshii Ryusaishin (thin spicy), Usbunia mushrooms, Usbenitachia mallow, phalaenopsis, crabs (summer hay), Udo or Shishido (Alive, Independent, Tang Dynasty), Unyadegato (Uncaria, Cat's Claw), Uba tea, Ume (Umecha "seed, pulp" ”), Vulcanus serrata, staghorn (Prickly pear), Satsuma mandarin (Chelsea), Unboku, Estragon, Ezoukogi (蝦 夷 五 加), Ezo lily of the valley, Echinashi (Hobasaki varengiku), Enishida, Enokitake (榎 茸), Ebisugasa ), El Campre, Elderberry “fruit”, Elemi, Elingi or Pre-Lotus Elinji, Enju (Summer, Satsuma Rice), Pea, Ogi or Kibanaogi (Yellow Twilight), Naruko Yuri or Kagakuruma Naruko Yuri (Yellow), Ohisanoyuki Is Sasanoyuki, Coptis (Coptis), Oogatahouken,
Great-crowned cucumber (Carocon), Greater walnut (Civilian incense), Greater fox swordfish (Preventive self), Larger octopus (Front car, Front car), Large thistle (Luozhou leakage, Bald eagle), Large horned whale (Large lion), Greater cactus, Greater lobster Okera (white birch), Greater crape myrtle (Banaba), Ooyabaya chabushi "fruit", Great white shrimp (cereals), Omia catechu, Barley hawthorn or Hawthorn (Yamako), Omi palm, Barley (barley), Oka, Okazeri (Octopus), Okra "Fruit", Oguruma (turnover), Oguri higotai (Zhengzhou leakage, indigo sashimi), Panax ginseng or Tochibaninjin (carrot), Hypericum pertussis or L. pertussis or Hypericum pertussis (brother cut grass), Odrichosium (interrupted), Onamomi Hearing child), Onigurumi, Onidoro or Tokoro or Nagadokoro (Hikai), Oni Yagara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onoeran, Ononis, Halibut (Kirihagi), Ominaeshi (defeated soy sauce), Oyuko, Dutch mustard (Cresson), Dutch jellies, Dutchville, Netherlands Honey Bees, Olives “Fruits, Seeds, Leaves”, Oregano, Orange “Fruits, Peels”, Silkworm (Mature Yellow), Cacao “Fruits, Peels, Seeds”, Oysters (柿蒂 “Leaves”), Kagi Kazura ), Kakidooshi or Kantori (Lotus herb), Kakiran, Gaku hydrangea, Cassia, Casino oyster (fruit berries), Gadju (Kashiwa), Kashiwa (Kashiwagi, Kashiwa), Cascarilla, Cascara grada, Gypsophila, Gypsophila , Cynomolgus (Kinshato), kaniwa, carnation, valerian (valerian), hippopotamus, birch Weeping birch (white birch), gerbera, pumpkin, Kapoknoki "seed", Japanese cabbage salamander (carp), catfish (carp yellow), chamomile or roman chamomile, chamomile (weed), camcome (camo duck), color, carax salmon, caladium or cinnamon cucumber (Wang Song), Crowed Bishak (half-summer), Oats, Garana "Seeds", Karaho (Koh-Pak), Karaya, Garyu, Karin (Kiso), Garcinia, Kawamidori, Kawara Psycho (Koryocho, white-leafed grass), Kawajisha, Kawaratake, Kawaranadeshiko (stone bamboo) or Ezokawaranadeshiko (buckwheat, buckwheat), Kawaraninjin (blue strawberry), Kawara mugwort (bamboo chin), Kansui (licorice), licorice (licorice), Kantarasa, Kandelira, Kanto, Kanna, Kanran , Raspberry (Ezo strawberry, Dutch strawberry, Shrimp Raspberry, Nawashiro strawberry, Momiji strawberry, European raspberry), Kiwi "Fruits, leaves", Kikarasuuri (Korone, Kikyo, Kikyo-root), Chrysanthemum (Chrysanthemum, Zhikangiku, Datura), Kikutaniku, Kisage (fruit) , Swordfish (sheep), pheasant (fruit), squirrel, spotted aloe, spotted mint, quina, linden (shinkona, red linden), quinoa (quinoa), yellowfin (yellow tuna), yellow bean, gymnema sylvesta, kimbek, unripe cabbage Fruits, caraway, cucumber, gyory (Nishikawa willow, taiyanagi), Kiraja saponaria, kiraya, Kiranso (Kinza small grass), kumquat "fruit", kinkiju, ginsekai, kimbusen, betel, kinzuhiki (Sentsukusa), quinlan , Ginlan, Kinryohen, Gua -Vera Vermela, Guava "fruit", Guayule, Quercus Infectria, Cucuinut, Kugenumaran, Cuco (Cultivation, coconut leaf, cocoon leaf, ground bark), Kusasu Kazura (Tenmon Winter), Kudzu (Kurdori), Camphor tree, gooseberry "fruit", carrot carrot (yellow flower moth), cutiti river, gardenia (wild lion), squirrel (song), cupuas, cubae, kumazasa, pine moth (clasp), clara (cranberry), cranberry ""Fruit", chestnut "seed, fruit, astringent skin", curkurigo latifolia "fruit", grapefruit "fruit and leaf", buckthorn, black scabbard (rescue), kamebahikoshi or kurobanahiokikoshi or hyokukoshi (extended grass), clove (clove, clove ), Gumbai Nazuna (Sekimei, Sekimeishi), Keigai (芥, 荊芥 穂), Celosia (chicken flower, hen), gekkavidin, bay laurel, Kenashi Sartorii rose (Dogo, mountain return), Gentiana, Genokosho (Oldgrass), Kemponashi (Kigushi), Kokisekoku, Kikumikan (Koji, Tachibana, Obanimikan, Fukurikukan, Sagamikouji, Ponkan, Santara (Tachibana)), Koshinbara (Monthly Flower), Kousei Hakka, Kozo "Fruits", Kocha (Tea), Kouhone (Riverbone), Kohon (Etsumoto) , Tang Dynasty), Koryang, Koryokukyo (Koryoen), Cilantro "fruit", Kooren (Huang Huang), Koganebana (Yellow Gon), Cowberry (Koshitachi), Coco "Fruit", Goshuyu (Kurei), Gosho Strawberry ), Pepper (pepper), cosmos, copaiba balsam, coffee "seed" , Leaves ", kobushi or magnolia (hot pepper), burdock (beef bowl, beef eggplant), kobotan lees, sesame (sesame seeds), sesame seeds (genus ginseng), trash beetle (poisonous rice), sanekazura or binankazura or matsubusa, wheat (wheat), rice or Rice bran “red cocoon, white birch”, rice oil, cola acuminata “seed”, cola bella “seed”, colony llya, fenugreek “fruit”, colombo, condurango, kombu, konnyaku, comfrey (candid grass), sisal ( Sisal Asa), Sai Hairan, Sakaneran (Ezosakaneran), Sasima button eel or Sinna button eel or Cinnamon (wei Sensen), Sakura (Oshima cherry, Yamazakura, Oyamazakura, Edhigashi, Bean cherry, Miyazakura, Yoshizakura, Takazakura, Takazakura, Takazakura, Takazakura Leaves, flowers, fruit, bark (Sakuragawa) "), cherry, pomegranate,
Sasa, Sasabaginran, Sasanka, Sasiomodaka (Sawamata), Sweet potato, Sugarcane, Sugar beet, Sunflower jujube, Saffron (Sanban flower, West safflower), Sapodilla, Sapotea mariyo, Pomelo "Fruit", Sabonso, Salmon berry "Fruit" Salamander (Sesame), Salamander (San indigo sashimi), Salvia (sage), Sagaki (mountain vegetables), Sawagurumi (wild walnuts), Sangaku cactus, Sangre de grad (croton), Sanshiyo-so, Carrot Ginseng), Sanshuyu (Yamamuro), Sansho (Yamamuro), Sandskon (Yamadone), Shia (Kalite), Cyanoki "Fruits", Shiitake (shiitake), Zion (purple strawberry), Sikakuma, Shikitaris, Sikkunshi Kimiko), Perilla or Aojiso, Chilemiso or Catamendiso Shiso leaves, shisoko), rosewood, linden tree, cinnamon onion, syringen (ream), shimeji (hinshimeji, shakashimeji, hatake shimeji, oshiroi shimeji, bunashimeji, honjimeshi, shirotamogitake), shimokren (spicy potato) ), Shajin (San Ginseng), Jasmine (Matsurika), Janohige (Wheat Gate Winter), Zodiac Cowpea, Shukukonkamiso, Shukushamitsu (Sanjin, Shredded Sand), Juzudama, Suro "Fruit", Shunlan, Zhouyou Palm, Ginger (Ginger), Zodiac Hydrangea (Tsuneyama), Shobu (Sardine, Sone), Shozu "fruit", Shirashi "Seeds", Silane (Bikkyu), Shirogori "Seeds", White clover (Clover), White-headed azuki (chicken bone grass) The white squirrels (Nio Iris), Shirobanatsuta "Flower", Shirobanupin, Shirominanten (Minami Tenshin), Cinconacill bra, Zinchoge (Mizuka, Mizuka, Senncho), Cinnamon, Cinnamomum cassia (Cinnamon), Watermelon (Sairan), Honeysuckle (Gold and Silver) Flower, Shinobi), Sorrel (Acid), Swimberry “Fruit”, Star Apple, Stevia, Strawberry “Fruit”, Suzu-Psycho (Xucho), Suginah (Question), Purslane (horse toothbrush, horse toothbrush), Plum "Fruit", Sulgaran (Oran), Japanese red pine "Bullet", Japanese larch, Japanese cypress, Japanese walnut, Japanese hawthorn, Japanese cypress, Japanese dandelion, Japanese horse chestnut (Maronier), Japanese pear "fruit", Japanese cypress Elder, Elk, Juniper, Juniper), Achillea millefol (milfoil), Rose, Pepper butterfly, Mistletoe serrata, Atlantic mint or Atlantic mint , Hongsekoku, Swankokukoku, Shikakekekukukoku, Kibananosekoku (Dendrobium, sarcophagus), Cedron (Boushuboku), Zeniaoi, Hirosanega, Senega, Seri, Celery, Senkyu (river cucumber), Senshinren (Denshinren), Sendan, Sennin Cactus, senna “fruit, leaf”, ginseng (large coral), sea bream (medicated), senboku, soka (grass and fruit), elephant chow, soshin-waby, buckwheat “seed”, pork potato, broad bean, Sulfur (daihuang), radish, soybeans, Daidai (orange peel, fruit), Takasago Gorhihitai (Southeast Indigo sashimi), Takasaburo (Cirrus lotus), Takatodai (Daisatsu), Takarabi (Cirrus), Dark Sweet Cherry " Fruits ", hollyhock, red-headed pearl millet (thyme, perilla), tachidokoro (hikai), tachiyanagi, tamarind" seed ", onion, ramshiba (hot pepper), taranoki" fruit, leaves, root bark ", tarayo (large leaf winter blue, one leaf tea, Bitter tea, bitter tea), dahlia (tenjiku button), tarwi, tanuki bean, tanshichiwa, tanjin (dansang), dandelion (Shangnang dandelion) or white dandelion or mouko dandelion, dammar, cherry "fruit", chigaya "fruit, root, Bud, Chixetsu Carrot (Bamboo Ginseng), Chicory, Chanka Piedra (Kidachikokankan), Chu Lip, Choco Stay, Artichoke (Artichoke), Artichoke (Large Yellow), Artichoke (Camellia), Artemisia (Camellia), Choreimaitake (Camellia), Chorogi, Tsukimiso, Tsukushi Kanelan, Tsukuritake (Mushroom), Azalea, camellia, camellia, clover (lacquered vine), Azalea (duck moth), Azuki bean (red bean), Azalea, Crested duck (what neck moth), Tsuruna (Apricot apricot), Carrot (Apricot), Apricot, Azalea, Deiko , Teuchigurumi, Duberry "fruit", Tengusa, Tencha (shade tea), Tendaiuyaku (glaze), Touga (winter coconut), Toka katsura, Capsicum (banban), Toki (toll), Tokinsenka (Marigold), Tosaikachi , Tounabena (river cut), corn Lokoshi or corn hair (Southern cocoon hair), Japanese hornworm (Sadako), Torindo or Chosen gentian (Dragon gall), Tokusa (wood band), Dokudami (10 medicines), Tokon (napping root), Toshishi or Mamedaoshi or Neshikazura, Tochu ( Tochu (bark, leaf, root)), ash (crust), tomato, tragacanth, triacanthus "seed", Turkish bellflower, tormentilla, dronoki, troro mallow, tonka bean, nigerian berry "fruit", Chinese yam or yam (mountain medicine), Nagyikada (Butcher Bloom), Naginata Kojunazuna, Rapeseed,
Japanese bean or vertical blade (sword bean), Natsumikan, jujube (Otsuchi), Naniwaibara (Golden cherry), Navena (continuous), Japanese squirrel, peanut (peanut), Nanten (Southern fruit), Nanban karamushi (Sanma), Niois violet, Nigaki (bitter tree), sagebrush (bitter melon), nutmeg, Kei or Nikkei or Ceylon nikkei or Yabunikei (Cinnamon) or Keishi (Katsura branch), nymph plover, elm (lion), elderberry (bone, "fruit, flower, stem , Leaves "), garlic (daegu), nulde (penta), leeks, nemno or nebu or nebi or neminoki or digokubana (community), noamis (daegu), nobara (fruits), nozenkazura (Lyoen flower), nogateto, yarrow, Nodake (frontal cucumber), wild rose, peach, pineapple "fruit", hibiscus (bussouge, furin) Rossou, Roselle, Hailindo, Hauchiwa, Pau Dorse, Hakama Labosi (bone fracture), Hakukayumatou, Hakusanchidori, Hakusen (white tinea), Hakuruberry "fruit", Jacobe (breeding), Hazel (palm), Hashiridokoro (Roth root), basil, lotus (lotus, lotus, lotus), parsley (holland jellied), hadakamugi, batata, wasp or mushroom (bamboo shoots), patchouli, mint (light load, light load leaf), yellow peas, yellow barley (yokuinin) , Hanasuge (my mother), Banana, Hanahaku, Hanabishi (Shitsuri, Shishitsuri), Vanilla bins, Papaya, Hakogusa (Paso), Pahalobobo, Hub "whole grass, stem, leaf", Paprika, Hamakakiran, Hamago or Mitsubahamago ( Vines), hamasge (kotsuko), sea bream (situation), hermanus (ma Kai flower), Hamaboufu (beach windbreak), Hamelis, Palm, Rose (Rose), Balata, Balata rubber tree, Agaric (Agaric, White-faced agaric, Ukimorinokasa), Haran, Pariurus (Prunus vulgaris), Parietalia, Harkanran, Balsamini ), Harunire (crusted bark, whitened bark, coral leaves), Barbasco, Harlindow, Handaikai (Atsumi Sea), Panoki, Hagi (Garden-dried), Pepper carrot (Participant), Pecan nuts, Higanbana (Ishibuchi, Bujusha) Hana), Higuramidaria, Higotai, Hishi (Hishimi), Pistachio, Beet, Hitsuba (Ishizuchi), Hitsubei Nishida, Daisies (Daisy), Hinata Kozuchi (cow knee), Japanese chicks, Cypress, Hiba, Castor, Sunflower, Pepper , Himeuzu (tengu), hime Catfish, Japanese pine bamboo (Kawariharatake, Hiromatsutake), Himemuyoran, Pimenta "fruit", Bikushi, Bikku, Ayuyu "Fruit", chickpea, Japanese lentil, Yellow-tailed oil, Hirohaokinagusa (bald eagle), Biwa "fruit, leaf, stem ”, Betel wax (large clothes, coconut), puer tea, prunus quail (southern wicker), buffalo, dandelion (striated winter flower, syllabary winter leaf), Fujibakama (Orchid grass), wisteria bean (blossom bean), wisteria beetle (butterfly), Grapes "fruit, pericarp, seeds, leaves", beech, Fuyumushinatsusatake (Cycium Cordyceps), Brazilian licorice, Brazilian carrot, Brazilian palm, Brazilian elephant palm, black currant "fruit", blackberry, buranyen, plum "fruit" ”, Full celaria, blueberry (Prunus japonica), pull , Floral blanca, blond psyllium, bundo (mung beans), hazelnut, loofah, hetsukaran, henken, belladonna, berry "fruit", persair, pervianburg, pereschia grandifolia, red croaker or juvenile berry (fruit) ”, Scallop or sardine or nendo or hahagigi kokia (skin pupa), spinach, spinach (saccharidum, acute pupa, teleost), pouteria sapota, pouteria rukuma, cypress, spinach, physalis ( Toro), Hoonoki (Japanese Kokaku, Pak), Bokeh (Kiso), Hosoba Aburagiku (Kayoyoku), Hosobaa Guruma, Hosobana Okera (Samurai), Hosoba Kirinso (Keiten 37), Hosobabarengiku, Bodaiju (Ogi), Button (peony "flower Leaf, stalk, bark "), button pi (peony skin), hop, jojoba, borage (Lulichisha), Bordeaux, white berry" fruit ", hononiku (meat juju, large art), honsekkok (iron skin stone wall, sparrow mountain) Sarcophagus), mizurutennansho (Tennansei), maitake (maiko), maou (mao), maca, macadamia nut, margaret (mokushungiku), macri (marine grass), mugwa (mulberry white bark "bark, leaf") , Magnolia splengeri, mazaran uba, masalan zuba de sear, masheira de boi, mashua (tamanozenhalen, kyukonkinrenka), matatabi (wood tengu), matsukasa, pine "leaves, bark, root", matta Orillo, Matsuhodo (茯苓), Matico (Cordon Sillo), Mayoram (Hanahakka), Malvano Jazine (Bitter), Ma Ruberry "fruit", quince, malein (broad-mulled squid), mango, mangosteen, manzanya (andesca mitsule), manshu gurumi, mandarin "fruit", garlic mushroom (ganoderma biloba), pheasant (fruit berry "fruit"), Mishima psycho (Shibako) ), Mizusobako or Mizuasagao (Drugosa), Mizokakushi (half-sided lotus), Misohagi (Chinese cucumber), Michiyanagi or Niwayanagi (Henshu), Mitsuwashi, Mitsuba, Midori Zapote, Mirihakka, Mimosa, Mioga, Miracle Fruit "Fruit" , Myrrh, myobaran, wheat straw, mugwort, mugwort, mugwort (prolonged skin), munya, murasaki (purple root), murasakikibu or oomurasakikibu (purple pearl), purple corn, murasakikinafuji (Kunming chicken blood wisteria), megakansa・ Optica, me Jiki (benusea), Mebougi, Melaroika, Melissa, Merirot, Melon "fruit", Moukomomogi, Mosouchiku, Mokkirin, Mogi (Pepper), Mochinoki, Mokko (woody incense), Momijidaio, Peach (Peach "leaves, seeds, Flowers, fruits "), bean sprouts, morero cherry" fruits ", Moroheiya (burlap), Yakawa Murasakiimo,
Yakuchi (masutachi), cornflower (cornflower), cornflower mint, yacon, yashabushi (arrow wheel) or cyprinus shabushi "fruit, pericarp, fruit ear", jade willow, scorpion (octagonal gold plate), mistletoe (willow parasite), willow River willow, chili willow, weeping willow, red willow, cat willow, Incolyana nagi, Kinu willow, Kolyanagi, Unryu willow, Miyama willow, palm, Yamanagi, Obayanagi, Tairukunuyanagi, foxglove, bee , Yabu tobacco (crane camellia, Tenmeisei), Japanese pokeweed (commercial land), Japanese alder (Yamamuro), Japanese yam, Japanese sagebrush, Eucalyptus, Yukinoshita (tiger ear grass), Yucca or flavifolia, Yuzu "fruit", lily Yoroi Saury, Artemisia (Leaf), Ligan (Raimaru), Lime "fruit", Rye, Lacanca "Fruit", Raspberry "Leaf, fruit", Latania (Clamelia), Lackey or Shallot, Lavender, Longan , Agave (Aonu Agave, Fukurin Agave), Ryokucha (Green Tea), Apple “Fruits, Seeds, Leaves, Roots”, Gentian, Lubus or Suavisimus (Lily Cool), Ruritama Thistle (Vulgaria typhoid, Xinjiang Ginseng), Reishi ( Toothpick, toothpick), lettuce (chisha), red currant “fruit”, red pitaya, lemon “fruit”, lemongrass, forsythia or Chinese forsythia (ream), forsythia, wintersweet, wax palm, locon Root), Loganberry "fruit", rosemary (mannenro) ), Rose hip (Novara), wasabi, Watafujiutsugi (dense Komuhana), burnet (Chinire), and the like.

  As seaweeds, seaweed [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (usba aonori, Sugiaonori, Hiraaoori, Boaonori, Hosoeda Aonori), seaweed [Brown algae] (Macombu, Resilibu, Hosocome, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis Integrifolia, Neocitus Ruetkeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Umiuchiwa, Akaba Umiuchiwa, Konami Uchiwa, Okinawa Uchiwa, Usuki Uchiwa, Etsumi Uchiwa], seaweed [Red Algae: Hijirimen, Maca (Tengusa), Hiluxa, Syria, Occasus , Kata-bucka, Yatabegusa, Yuukiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusakirinsai, Giraffe rhinoceros, Scots-billed Rhinoceros, Tsunomata, Otsuno-no-Mata, Tocha-ka-no-no-Tama, Togetu-no-Tata Typical examples include Kainori, Yaleus banori, Kagios banori, Susius banori, Haius banori, Akamominori.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kuwanomimo, Volbox: Dandelion, Volbox, Palmera, Yotsumeo, Himidori Aoumiduro, Tsurugimidro, Hibimiduro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa: Ooshiogusa, Asamirioshigusa, Kawasiogusa, Marimo, Valonia:
Tamagobaronia, Tamavalonia, Magamamomo: Magamatamamo, Iwata, Genoa, Serichogita, Heraiiwata, Kurokita, Hanemo, Genus: Mill, Chromil, Sakibuchimiru, Nagamil, Hiram, Casanozu, Zumo , Mika genus, Collecaete genus, Tsutsumimo genus, Dendrobium genus: Dendrobium genus, Human genus genus: Human exercising, Hirono human eugosa, Ushi human exercising, Japanese crested genus, Sayamidro genus, Kumiko genus, Sumiremo genus, Hemimido genus, etc.)

  Cyanobacteria (Suizenjinori genus: Suizenjinori, Aoko genus, Nenjumo genus: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Aiacacio), etc.).

  Brown alga Sanadagusa: Sanadagusa, Fukurimamiji, Commongusa: Commongusa, Yahazugusa: Ezoyahaz, Yahazugusa, Uraboshiyahaz, Jigamusa: Jigmygusa, Umichiwagusa, Umamisakugakua, Hakusou : Navarimo, Chasomen genus: Motsuki Chasomen, Matsumo genus: Matsumo, Nagamatsumo genus: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, fake Tomozuku: Niseft Mozuku, Futomozuku: Futomozuku, Ishimozuku: Ishimozuku, Kuromo: Kuromo, Nisemozuku: Nisemozuku, Mozuku: Mozuku, Ishige: Ishige, Iroro, Ichimegasa: Ichimegasa Umibosu, Urushigusu: Urushigusa, Keurushigusa, Tobaccogusa, Composita: Composita, Haberodomo: Haberodoki, Havanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukuro Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: yokojimamanori, Ganoderma genus: cynomolgus, fennel spider: fennel spider, vine genus: turmo, turtle genus: sea turtle, scorpion genus: sujime, misji kumbu genus: misji kumbu, atsuba misji kumbu, kumbu genus: tsutsugara kumbu, oyster kumbu, onikombu, gakonbubu Barracuda, Trollocombu: Trollocombu, Antokume: Antokume, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishicombu, Nejikcombu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainuwakame, Chigaiso, Oniwakame Ezoi Shige: Ezoi Shige, Yabane Moku: Yabane Moku, Rappa Moku: Rappa Moku, Joromoku: Ugano Moku, Jo Lomoku, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsukuri, Yatsumata Moku, Umitora no Moku, Fushishimoku, Mohmokumoku, Hahamokumoku , Ukimo: Ouokimo, Burukimo: Burukimo, Kayamonori: Kayamonori, etc.).

  Red algae Genus: Sea urchins, Tsukumonori, Anemone crickets, Astragalus, Amaranthus, Amaranthus, Agaricata Genus: Tamaitadaki, Kaikekenori Genus: Kaginori, Kaikekenori, Tengusa: Himetengususa, Hytengususa, Oobusa, Nambugu , Kohira, Yohira, Kinukusa, Hibiro, Genus: Hibirodo, Himebiro, Isomumedo: Isommedo, Amphidia: Rhizome, Ryumonso Naminohana: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanawa, Kinokawa: Kinokawa, Kanonote, Sangori Kyono Shiro, Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Fudarak, Marubahdarak, Isonohana: Nurakusa, Ronulaksa, Oomkadenori, Hirakintoki: Hirakintoki, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Rice, Tokamatsu, Hitomatsu, Fushikintoki, Tsunomude, Nagakinito, Crayfish Nagaobane: Naganobane, Funori genus: Hanafonori, Fukurofunori, Mafunori, Karekigusa: Karekigusa, Tosakamodoki: Hosobanatosakamodoki, Hironotosakamodoki, Yatsudegatatosakamodoki, Kurotosakamodokisane, Zotosofomoza Tosakamodoki, Nankaitosakamodoki, Himetosakamodoki, Kinuhada: Kinuhada, Ezotosaka: Ezotosaka, Tsukasanori: Enashikarimenia, Otsukasanori, Hanaga Tacarimenia, Hoonoo: Honoo, Hikagenoit: Hikagenoit, Usuginu, Nikuhounoo: Nikuhounoo, Venus nago, Venus nago, Susca veni: Suscabeni, Okarugusa: Yamadagusa, Mirin: Mirin, Hosokamiso , Isomocca genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagibaranori, Kojinoo: Kizino-o, Isodantsu: Isodantsu, Atsubanori: Atsubanori, Ogonori, Ogonori, Ogori Mizo-okonori, birch, beniogonori, fukureshinori, purple birch, shinkaibanori, spruce birch, kataogonori, ryukyuogono , Scorpionfish, tsutsuginu, yumigataonogori, kubireogonori, mosagogonori, kinubanori, genus genus: beetle genus, genus genus, genus genus , Rhinoceros, spinach, spinach genus: spinach, cedar genus: squid, reeds, hornwort, spiders Genus: Taoyagisou, Hanasakura, Fukurotsunagi Genus: Fukurotsunagi, Sugikonori, Hananoeda Genus: Hananoeda, Hiratakaoyagi: Hiratakaoyagi, Darus : Dulse, Scorpionus, Anadalus, Weibusa: Uebusca, Benifronori: Benifuronori, Fusunagi: Futsutsunagi, Himefusunagi, Hirohafushitsugi, Otsunagisou, Hirawatagigi, Usuwasugi , Egonori: Egonori, Futogigi, Saeda: Saeda, Chirimomiji: Chirimomiji, Konohori family: Habutaenori, Konohori, Suzuronori, Usubeni: Genus: Kagios vanori, Yareus vanori, Susius vanori, Heius vanori, Usbanori modoki: Usbanori modoki, Ayanishiki: Ayanishi Genus: Aeginu, Dadia: Enasidadia, Shimadadia: Isohagi, Shimadadia, Dajimodoki: Dajimodoki, Euglena: Morayogusa, Fuyugusa, Makuri: Makri, Yanaginogi, Yuna, Yukinagina , Sasabayanaginori, Sozo genus: Kurosozo, Kobsozo, Hanesozo, Sozonohana, Hanegusa genus: Hananegusa, Hananegusa, Kozanemo genus: Kozanemo, Isomurasaki, Hosozozanemo, Himegoshigusu, Giant genus Isomagusa, Suginasugusa: Suginasusa, Isobashou: Isobasho, Fujimatsumo: Fujimatsumo, Sawweed: Haeshinosakiba, Kawamoku Kawamozuku, Aokawamozuku, Himekawamozuku, Ideyukogome genera: Ideyukogome, Okichimozuku genera: Okichimozuku, Itogusa genus, Chinorimo genera: Chinorimo, Chisujinori genera: Chisujinori, etc.).

  Axial algae (Shajikumo, Shiratamamo, Hoshitsurimo: Hoshitsurimo, Rikunotumusu, Flaskmo: Himebumo, Chaboflasmo, Tripera, etc.), yellow algae (Hikarimo: Hikarimo, etc.), etc.

  In addition, the materials derived from animal materials include chicken crown extract, cow or pig, human placenta extract, cow or pig stomach, duodenum, intestine, spleen extract or decomposition product thereof, cow or pig brain tissue Extracts, cattle / pig or fish collagen hydrolyzate (acid, alkali, enzyme etc.) degradation products, water-soluble collagen or collagen derivatives such as acylated collagen, cow or pig elastin or elastin hydrolyzate (acid, alkali, Enzymes etc.) or water-soluble elastin derivatives, keratin and its degradation products or their derivatives, silk proteins and their degradation products or their derivatives, porcine or bovine blood cell protein degradation products (globin peptides), cattle or porcine hemoglobin degradation products (Hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and its degradation products or their derivatives, skim milk powder and their degradation products or their inducements Body, lactoferrin or decomposed product thereof, egg ingredients, fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.

  As a material derived from a plant or animal material, any site, cell, tissue, organ, metabolite, etc. derived from a gene recombinant or a cell fusion can also be used. Furthermore, for example, an undifferentiated cell group, a cell group in the middle of differentiation, or the like obtained by culturing an arbitrary site, cell, tissue, organ or the like can also be used.

(14) Marine components Seawater such as deep seawater, for example, sea salt, dried seawater, dead sea or Atlantic or Pacific sea water (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud ( Fango), for example, Italian mud, German fungo, Eifelfango, Freiburg fungo, etc. Sea mud or mud from various locations (containing components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, Magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, etc.

(15) Microbial culture metabolite Yeast metabolite, yeast extract, bacterial metabolite, bacterial extract, mold or actinomycete metabolite, mold or actinomyces extract, natto metabolite, natto extract, rice fermentation extract , Fermented rice bran (red rice bran, white birch) extract, Euglena extract or decomposition product thereof or water-soluble derivative thereof, trehalose or derivative thereof, lactic acid fermentation product of raw milk or skim milk powder, fermented lactic acid bacteria of legumes, coconut plant Lactic acid bacteria fermented products.

(16) α-hydroxy acids Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(18) UV absorber / blocker benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxy Dimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), paraaminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, Octyl paradimethylaminobenzoate), methoxycinnamate derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, paramethoxycinnamate) Octyl, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate) , Ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (such as methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, Imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allanto Derivatives, nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, cinoxalate, oxybenzone, dioxybenzone, octabenzone, soribenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, Escalol, zinc oxide, titanium oxide, talc, kaolin, etc.

(19) Whitening agent Paraaminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone derivative, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, Shikonin, baicalin, baicalein, berberine, placenta extract, ellagic acid, lucinol, etc.

(20) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or a derivative thereof, vitamin E or a derivative thereof, N-acetyltyrosine or a derivative thereof, hydroxytyrosol or a derivative thereof, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (camomile, mulberry, gardenia, toki, Barbet, Clara, Mugwort, Honeysuckle, Yellowfin, Dokudami, Matsuhodo, Barley, Olysam, Hops, Hawthorn, Eucalyptus, Achillea millefolium, Artea, Keihi, Mankeishi, Hamamelis, Yamamaguwa, Lifelong grass, Kikyo, Toshi Shii, tsuyoiko, ganoyu, mao, senkyu, dokatsu, psycho, boufuu, hamabofu, ougon, peony, peonies, genus shochu, kuzukone, licorice, quintuplets, aloe, shouma, safflower, green tea, black tea, asen-yaku).

(21) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether).

(22) Turnover promoting action / cell activation substance hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, Ogon, Japanese horsetail, Gentian, Burdock, Shikon, Carrot, Camelis, Hop, Yokuinin, Odori, Semburi, Japanese cypress, Japanese red snapper, Achacha, Hypericum, Cucumber, Necklace, Rosemary, Parsley).

(23) Astringents Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenol sulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds) and the like.

(24) Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(25) Antioxidant Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol or a derivative thereof, Parahydroxyanisole, propyl gallate, sesamol, sesamorin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (sesame culture cell, hamcha, hypericum, hamamelis, clove, melissa, birch, birch, salvia, rosemary, minamitenji, ginkgo, green tea).

(27) Anti-inflammatory agent Itamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or the like Salt, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract, propolis, etc.

(28) Antiseptic / antibacterial / bactericidal / disinfectant Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or its hydrolyzate, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, salicy powder, iodine compound, iodoform, sorbic acid or salt thereof, propionic acid or salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol , P-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, O-phenylphenol, Irgasan CH3565, halocarban, f Xachlorophene, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactant ( Polyoxyethylene lauryl ether, polyoxyethylene nonylphenyl ether, polyoxyethylene octylphenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyl bromide) Trimethylammonium, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine, brilliant Lean, malachite green, crystal violet, Jamaru photosensitive No. 101-containing, photosensitizer No. 201, photosensitizer No. 401, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.

(29) Moisturizer Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or its derivative or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, mini Sasanishiki extract, natto metabolite, natto extract, loofah water, birch (white birch) or red pine sap

(30) Elastase activity inhibitor Fluorophosphate diisopropyl, plant extract (Ogon, Hypericum, Clara, Mulberry leaf, Keihi, Genso pepper, Comfrey, Salvia, Elderberry, Bodaige, Buttonpi), seaweed extract and the like.

(31) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thio Glycolic acid and the like.

(32) Anti-androgen agents Follicular hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, oxendron, etc.

(33) Peripheral vascular blood flow promoter Vitamin E or derivatives thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.

(34) Stimulant Pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(35) Metabolic active agent Photosensitive element 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placental extract, biotin, pentadecanoic acid glyceride and the like.

(36) Antiseborrheic agent Pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.

(37) Keratolytic agent Resorcin, salicylic acid, lactic acid, urea and the like.

(38) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(39) Hair remover Inorganic reducing agent such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or its salts (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate) Strontium thioglycolate) and the like.

(40) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(41) Dye agent 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, hydrochloric acid 2 , 4-Diaminophenoxyethanol, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine , Paraa Minophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorcresol sulfate, 2-amino-5-nitrophenol sulfate, orthoamino sulfate Phenol, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, sulfur Paranitroorthophenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid , Resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(42) Fragrance Natural animal fragrances such as musk, civet, castorium and ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamoc Tomato essential oil, Peruvian balsam essential oil, Boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essence essential oil, etc. Other synthetic fragrances.

(43) Dye / Coloring Agent Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin etc.

(44) Others Hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, cooling agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides And its degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound treatment agents, foam increasing agents, thickeners, enzymes, purified water (electronic Water, small clustering, etc.), oral preparations, deodorizing / deodorizing agents, bittering agents, seasonings, etc. can be raised and used in combination with these to provide additive or synergistic effects of the present invention or other cosmetic effects. Improvement can be expected.
Other examples of (1) to (43) above or examples of (44) include sodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, tranexamic acid and Its derivatives, glabrizine, fire spines, tocopherol acetate.

  Although this invention is illustrated below, it is not limited to it.

<Reference example>
The petals of safflower were diluted with physiological saline and cultured on a potato dextrose agar medium supplemented with chloramphenicol at 28 ° C. for 3 days. The grown colonies were picked and cultured on potato dextrose agar medium and YM agar medium at 28 ° C. for 3 days. It was confirmed by microscopic observation that the grown cells were yeast, and it was identified as Pichia kudriavzevii from the results of 26S rDNA-D1 / D2 nucleotide sequence analysis of this yeast.
<Production example>
Yamagata Prefecture safflower (Carthamas tinctorius Linne (Compositae) petals were extracted with hot water to remove solids to obtain a safflower extract.
In this safflower extract, yeast isolated from safflower (Pichia Kudriavzevii) (Depositing organization: National Institute of Technology and Evaluation, the Depositary Microorganism Depositary Center, Deposit Date: September 4, 2012, Deposit Number: NITE) After inoculating 1 wt% of P-1414) and culturing at 30 ° C. for 3 days, the culture was sterilized at 100 ° C. for 30 minutes, centrifuged to remove insoluble solids, and the following stock solution for safflower fermented broth (Referred to as safflower fermentation broth stock solution).
Phenoxyethanol (3.0% by weight) and 1,2-pentanediol (0.5% by weight) were added to the safflower fermentation liquid stock solution, and then centrifuged to remove insoluble solids. A solution containing safflower fermented product used in the following test (referred to as safflower fermented liquid) was obtained.

<Example of confirmation>
Color / Phase The safflower fermented liquid was a pale yellow to brown liquid.
Odor The safflower ferment had a unique odor.
Reducing sugar 3 mL of a failing test solution was added to 5 mL of safflower fermented liquid and heated in a water bath for 3 minutes to produce a red precipitate.
When 2 mL of ninhydrin test solution was added to 5 mL of the amino acid safflower fermented solution and heated in a water bath for 5 minutes, the solution turned purple.
pH
The pH of the safflower fermented liquor was 4.0 to 6.0.

<Test example>

Stability test The stability of the safflower fermented liquid to heat and / or light was tested. As a result, no change in pH due to heat and light was observed. As for the appearance, a slight increase in absorbance was observed due to heat, and significant fading due to light was also observed. In addition, no abnormalities such as the occurrence of starch were observed.

Collagen production promotion test

Sample Test sample: Safflower fermented liquid (diluted with purified water if necessary)
Control: Purified water

Test method 1. Normal human dermal fibroblasts were used as precultured cells. As a culture solution, DMEM medium (SIGMA) containing 5% fetal bovine serum (manufactured by Thermo Tace) is used, cells are seeded on a 24 well culture plate at a concentration of 2 × 10 ⁴ cells / ml, and placed in a CO ₂ incubator at 37 ° C. For 72 hours.
2. Sample addition After removing the pre-culture medium, DMEM medium (SIGMA) containing 0.25% fetal bovine serum (manufactured by Thermo Trace) is used as the main culture medium, and 1 mL of main culture medium per well on a 24-well culture plate. Was replaced. After 24 hours, the medium was replaced again with the main culture medium, and a sample (added so as to have a concentration of 10% by weight in one group and added to a concentration of 50% by weight in the other group) was added. Then cultured for 72 hours at CO ₂ incubator at 37 ° C..
3. Measurement The amount of type I collagen produced in the culture supernatant was measured using a Procollagen Type I C-Peptide (PIP) EIA Kit (TAKARA) that can quantify Procollagen Type I C-Peptide (PIP). The number of samples was measured with n = 3, and the average value was used. Other operations were in accordance with the operation method attached to the EIA Kit.

Results Assuming that the amount of collagen produced in the control group was 100%, the production amount was 230% in the group of 10% safflower yeast fermentation solution and 412% in the group of safflower yeast fermentation solution 50% (FIG. 1). ).

Fibroblast proliferation test

Sample Test sample: Safflower fermented liquid (diluted with purified water if necessary)
Control: Purified water

Test method a. Cells and culture conditions Human fibroblasts were used as cells. In the culture solution, DMEM medium (SIGMA) containing 5% fetal bovine serum (manufactured by Thermo Tace) is used as a pre-culture medium, and 0.25% fetal bovine serum (manufactured by Thermo Tase) is used as the medium for this test. using DMEM medium (SIGMA) containing, cultured under the condition of 5% _CO 2, 37 ℃.
b. Culture and measurement 4 × 10 ³ cells / well of fibroblasts were implanted in a 96-well plate and pre-cultured for 48 hours. Thereafter, the medium was replaced with a fresh medium, and the sample was added to the culture medium. After culturing the cells for 3 days, the number of fibroblasts was measured by Cellcounting kit-8 (DOJINDO).

Results The cell number (Cell Index) of the test sample addition group when the number of control cells was 1 was 1.123 (FIG. 2).
A fibroblast proliferation test was conducted using 0.1%, 0.5% and 1% of the unfermented safflower extract (Falcolex (trademark) safflower B) as a test sample. (Not shown).

Keratinocyte proliferation test

Sample Test sample: Safflower fermented liquid (diluted with purified water if necessary)
Control: Purified water

Test method a. Cells and culture conditions Normal human epidermal keratinocytes (Kurabo KK4009) were used as cells. In the culture solution, Kurabo Industries EpiLife KB2 (including insulin, hEGF, hydrocortisone, antibacterial agent and BPE) medium is used as a preculture medium, and hEGF and BPE are removed from the preculture medium as a medium for this test. Used. Both the pre-culture and the main test culture were cultured under conditions of 5% CO ₂ and 37 ° C.
b. Cultivation and Measurement 1 × 10 ⁴ normal human epidermal keratinocytes were seeded on a 24-well plate and pre-cultured for 24 hours. Then, it replaced with the culture medium for this test, and added the sample in the culture solution. After culturing the cells for 6 days, using Cellcounting kit-8 (DOJINDO), Abs. 450 nm O.D. D value was measured.

Results The cell number (Cell Index) of the test sample addition group when the number of control cells was 1 was 1.084 (FIG. 3).
In addition, the keratinocyte proliferation test was conducted using 0.1%, 0.5% and 1% of the unfermented safflower extract (Falcolex (trademark) safflower B) as test samples. (Not shown).

Antioxidant test

Sample Test sample: A safflower fermentation stock solution diluted with purified water to a volume 15 times (including 3.0% of 1,2-pentanediol and 0.5% of phenoxyethanol)

Test Method Each sample was tested in the DPPH radical scavenging activity test.
The sample solution, ascorbic acid solution ^{* 1} and 0.5 mL of water were accurately taken in a test tube, and 2.5 mL of 0.1 M acetic acid-sodium acetate buffer solution (pH 5.5) was added and stirred. Further, 2.0 mL of DPPH (1,1-diphenyl-2-picrylhydrazine) solution (0.25 mM in ethanol) was added and stirred, and allowed to stand at room temperature for 10 minutes, and then the absorbance at 525 nm was measured.
In addition, the sample solution, ascorbic acid solution ^{* 1} and 0.5 mL of water were accurately taken in a test tube and operated in the same manner as described above, and ethanol was added instead of the DPPH solution to correct the absorbance ^{* 2} .
* 1... In order to obtain a numerical value of 0, a positive control was obtained by dissolving ascorbic acid in the same solvent as the sample so as to be 1000 μg / mL.
* 2: To correct the coloring of the sample solution, ascorbic acid solution and water.
The inhibition rate was calculated by the following formula.
Erase rate (%) = C−S / C × 100
C: Control absorbance-positive control absorbance S: Sample absorbance-positive control absorbance

Results The test sample showed an erase rate of 14.85%.

Tyrosinase activity inhibition test

Sample Test sample: A safflower fermentation stock solution diluted with pure water to a 15-fold volume (including 3.0% of 1,2-pentanediol and 0.5% of phenoxyethanol)
Control: Purified water

Test Method Each sample was tested in a mushroom tyrosinase activity inhibition test.
Test 1.0 mL of L-tyrosine solution (300 mg / L) and 1.0 mL of Mclvaine buffer (mixed with 0.1 M citric acid aqueous solution and 0.2 M disodium hydrogenphosphate aqueous solution to pH 6.5). It took to a pipe | tube and heated for 10 minutes with a 37 degreeC thermostat. 1.0 mL of the sample solution or blank solution was accurately added, 0.1 mL of tyrosinase solution (2500 units / mL) was further added, and the mixture was heated in a constant temperature bath at 37 ° C. for 20 minutes, and immediately the absorbance at 475 nm was measured.
In addition, the absorbance was measured in the same manner for a solution in which 0.1 mL of water was added instead of the tyrosinase solution.
The inhibition rate was calculated by the following formula.
Inhibition rate (%) = 100− (B−B ₀ ) / (A−A ₀ ) × 100
A: Absorbance of blank solution B: Absorbance of sample solution A ₀ , B ₀ : Absorbance when water is added instead of tyrosinase

Results The test sample showed an inhibition rate of 12.71%.

Moisturizing test

1. Moisturizing test using epidermis stratum corneum moisture measuring device

Sample Test sample: Safflower fermentation broth Solvent control: Aqueous solution containing 3.0% 1,2-pentanediol and 0.5% phenoxyethanol

Test method Using a skin stratum corneum moisture measuring device (SKICON-200, IBS Co., Ltd.), the electrical conductivity of the skin (30's male, forearm bent side inside) was measured with an electrode having an outer diameter of 6 mm and a center diameter of 1 mm. .
The electrical conductivity of the measurement site was measured in advance. Next, a circular filter paper having a diameter of 2 cm containing a sample was affixed to the fixed site, and after 1 minute, the filter paper was peeled off, and immediately after wiping off the excess sample, the electrical conductivity was measured every 30 seconds (room temperature 20. (Measured in a constant temperature and humidity chamber at 0 ° C., humidity 50.0%, and no wind).

Results The test results are shown in Table 1 and FIG.

2. Moisturizing test using moisture meter

Sample Test sample: Safflower fermentation broth Solvent control: Aqueous solution containing 3.0% 1,2-pentanediol and 0.5% phenoxyethanol

Test Method A moisture meter Cornerometer (CM825 probe, Curage + Khazaka) was used to measure the amount of moisture on the skin surface (30's male, forearm flexion side inside).
The amount of water at the measurement site was measured in advance. Next, a circular filter paper having a diameter of 2 cm containing a sample was affixed to the fixed site, and after 1 minute, the filter paper was peeled off, and immediately after wiping off the excess sample, the water content was measured every 30 seconds (room temperature 20.0). (Measured in a constant temperature and humidity chamber without air and 50.0% humidity).

Results The test results are shown in Table 2 and FIG.

  The safflower fermented liquor of the present invention has an increased content of many minerals, vitamins and amino acid components as compared to the safflower extract before fermentation. Although this invention is not restrained by theory, it is estimated that the effect of this invention originates in these component content increase.

<Prescription example>
Although the formulation example of the external preparation for skin containing the safflower fermentation liquid of this invention is shown below, the formulation example of this invention is not limited to it.
In addition, all compounding quantities are shown by the mass% with respect to the composition whole quantity.

Formulation Example 1 (Lotion) (mass%)
Glycerin 2.0
Propylene glycol 8.0
1-3 Butylene glycol 3.0
Polyethylene glycol 600 3.0
Pyroglutamic acid isostearate polyoxyethylene glyceryl (25E.O)
0.1
Citric acid 0.01
Sodium citrate 0.05
Monopotassium phosphate 0.01
Disodium phosphate 0.03
P-Hydroxybenzoic acid ester Hydrolyzed collagen solution 0.1
Hyaluronic acid solution 0.1
Safflower fermented liquor 20.0
Purified water residue

Formulation example 2 (milky lotion) (mass%)
Squalane 2.0
Macadamia nut oil 2.0
Methylphenylpolysiloxane 0.2
Cholesteryl hydroxystearate 0.2
Tocopherol 0.1
Polyglyceryl monolaurate 0.4
Glycerin 10.0
1-3 Butylene glycol 8.0
Xanthan gum 0.2
Carboxyvinyl polymer 0.2
L-Arginine 0.2
Hyaluronic acid solution 0.1
P-Hydroxybenzoic acid ester Ethyl alcohol 2.0
Safflower fermented liquid 30.0
Purified water residue

Formulation Example 3 (Cream) (mass%)
Squalane 12.0
Glyceryl trioctanoate 2.5
Stearic acid 1.5
Cetostearyl alcohol 2.0
Cetyl palmitate 3.0
Tocopherol 0.1
Dimethylpolysiloxane 1.0
Glyceryl stearate 2.0
Polyoxyethylene (20) stearyl ether 1.0
Polyoxyethylene (25) cetyl ether 1.5
Polyoxyethylene (10) cetyl ether 1.0
Propylene glycol 10.0
Glycerin 5.0
Safflower fermented liquor 10.0
Hydrolyzed collagen solution 0.1
P-Hydroxybenzoate appropriate amount purified water remaining

  All of the external preparations for skin of these prescription examples exhibited the skin activity effect of the safflower fermented liquid and were effective as a moisturizing agent.

Claims (8)

  A safflower fermented liquor containing a safflower extract fermented with yeast of the order Saccharomyces.   The safflower fermented liquid of Claim 1 whose yeast is Pichia (Pichia).   The safflower fermented liquid of Claim 2 whose yeast is Pichia kudriavzevii (Pichia kudriavzevii).   Cosmetics containing the safflower fermented solution according to any one of claims 1 to 3, collagen production promoter, fibroblast proliferating agent, keratinocyte proliferating agent, antioxidant, tyrosinase activity inhibitor or moisturizing agent . A method for producing a safflower fermented liquor, comprising:
Including fermenting safflower extract with yeast,
The yeast is Saccharomyces (Saccharomyces),
Method.   6. The method according to claim 5, wherein the yeast is Pichia.   The method according to claim 6, wherein the yeast is Pichia kudriavzevii.   Use of the safflower fermented solution according to any one of claims 1 to 3, wherein the cosmetic, collagen production promoter, fibroblast proliferator, keratinocyte proliferator, antioxidant, tyrosinase activity inhibitor Or use to produce humectants.