KR20140090633A - 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 - Google Patents

포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 Download PDF

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KR20140090633A
KR20140090633A KR1020147013195A KR20147013195A KR20140090633A KR 20140090633 A KR20140090633 A KR 20140090633A KR 1020147013195 A KR1020147013195 A KR 1020147013195A KR 20147013195 A KR20147013195 A KR 20147013195A KR 20140090633 A KR20140090633 A KR 20140090633A
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glu
ala
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ile
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루쓰 모이지
앤드류 존 헤론
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옥스포드 나노포어 테크놀로지즈 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
KR1020147013195A 2011-10-21 2012-10-18 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 Withdrawn KR20140090633A (ko)

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US201161549998P 2011-10-21 2011-10-21
US61/549,998 2011-10-21
US201261599244P 2012-02-15 2012-02-15
US61/599,244 2012-02-15
PCT/GB2012/052579 WO2013057495A2 (en) 2011-10-21 2012-10-18 Enzyme method

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KR20140090633A true KR20140090633A (ko) 2014-07-17

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US (5) US9758823B2 (https=)
EP (2) EP2768977B1 (https=)
JP (1) JP6226869B2 (https=)
KR (1) KR20140090633A (https=)
CN (1) CN104039979B (https=)
AU (1) AU2012324639B2 (https=)
BR (1) BR112014009579B1 (https=)
CA (1) CA2852812A1 (https=)
WO (1) WO2013057495A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200020928A (ko) * 2017-06-29 2020-02-26 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 나노포어를 통한 폴리머의 결정적 스텝핑

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