KR20140059419A - Cosmetic composition comprising fermented oil extract of oriental medicine fruits - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising fermented oil extract of oriental medicine fruits, and more specifically, to a cosmetic composition containing fermented oil extracts of schizandra, lycium, ginseng fruits and Ligustrum japonicum fruits as active ingredients thereby having effects of anti-allergy, anti-oxidation in cell and skin reproduction.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing an oriental herb extract fermented oil extract,

The present invention relates to a cosmetic composition having an effect of improving skin barrier, inhibiting anti-allergy, inhibiting intracellular oxidation, and regenerating skin, comprising an extract obtained by extracting Oriental fruit with fermented oil as an active ingredient.

Humans use cosmetics for cleanliness, beauty and skin care away from sexuality and age, and furthermore, they use cosmetics to change their skin to express beauty. Therefore, the functions of cosmetics are gradually improving, and as the industry develops, cosmetics become more luxurious and diverse. As the cosmetics industry grows, we want to use safe materials that are harmless to humans. In particular, natural materials including natural products and medicinal plants are being developed.

Skin is the largest organ of our body. It occupies about 16% of the whole body volume. It is in direct contact with the external environment and has many deadly harmful factors to infiltrate into the body. External stimuli such as temperature, humidity and ultraviolet rays and harmful microorganisms And protects the human body from damage. However, as we get older, natural aging and various pollutants, strong ultraviolet rays, stress and nutritional deficiencies can damage our skin, which prevents skin cells from functioning properly, Skin aging phenomenon such as generation, elasticity loss, and keratinization occurs. In addition, skin moisturizing and skin keratinization rates are also caused by skin aging. That is, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the dermis matrix, which plays an important role in maintaining skin elasticity with age, is reduced and the amount of insoluble collagen is increased, The dermis becomes difficult to maintain the moisture of the skin, and the skin becomes rough. In addition, sebaceous glands and sweat glands decrease in function, sebum and sweat are gradually reduced, sebaceous membranes are not properly produced, and protection for skin moisturization is lost. In the end, the skin becomes dry and translucence, And the elasticity of the skin protein is reduced and wrinkles are produced. In other words, the loss of moisture in the skin is a major symptom of aging, together with the reduction of skin elasticity and wrinkle formation, and is closely related.

In addition, it is known that wrinkle formation is caused by the production of reactive oxygen species, which is the main cause of skin damage when human skin cells are exposed to ultraviolet rays (UV-B, UV-A). Collagen and elastin, which are present in the dermis of the skin structure, are the major proteins that govern the elasticity of the skin. The biosynthesis of the collagen fibers receives internal and external nutrients of the skin. Internally, natural aging (reduction of estrogen) causes decrease of cell activity and decrease of collagen fiber. As external factors, active oxygen (free radicals) generated by overexposure of ultraviolet light and stress may react with the -SH group of the protein to inhibit the activity of enzymes, or enzymes that decompose collagen, elastin, etc. (Matrix Metalloproteinases: MMPS) collagenase, thereby inducing aging which reduces the wrinkles and elasticity of the skin.

To date, cosmetics or skin protectants containing various skin protecting ingredients such as retinol, retinoid, vitamin C, flavonoid and tocopherol have been developed in order to inhibit or slow down the aging of the skin. However, Has a weak potency in the final formulation and has a problem of severe skin irritation.

Recently, cosmetic products using natural vegetable raw materials, which are more safe and irritating to the skin due to the skin irritation problem of these raw materials, have been developed. Especially, in Korea, a lot of cosmetic products using natural oriental herbal ingredients are being developed. This is because the natural oriental herbal ingredients have long been used in the private sector, and safety and efficacy have already been secured. It is because.

On the other hand, fatty acids are divided into saturated fatty acids and unsaturated fatty acids. Saturated fatty acids are very stable because they do not have double bonds that can bind to and react with other substances and are mostly hardened at room temperature. Unsaturated fatty acids are fatty acids with double bonds, usually liquid, and the degree of unsaturation is determined by the number of double bonds. High unsaturation is called highly unsaturated fatty acid. The higher the degree of unsaturation, the more the number of double bonds becomes unstable.

These edible oils and fats provide an important source of energy and at the same time provide the unsaturated fatty acids necessary for physiological functions, and are very important not only in nutrition and physiology but also in a variety of foods and rich flavors. However, due to the unstable chemical nature of unsaturated fatty acids, the essential fatty acids themselves are destroyed due to the effects of peroxides and free radicals formed by the easy rancidity of the edible oil, and the oxidation stability is decreased, The inhibition of rancidity is a very important issue for the contained product.

The lack of linoleic acid and linolenic acid causes growth inhibition, skin or kidney failure, and decline in fertility. This is because linoleic acid is gamma linolenic acid, which is converted to arachidonic acid by gamma linolenic acid, Lt; RTI ID = 0.0 > prostaglandin < / RTI > Especially, as the correlation between the onset of atopic diseases and the deficiency of gamma linolenic acid has been revealed, it has become very important to appropriately supply unsaturated fatty acids.

However, unsaturated fatty acids, which are essential for a healthy lifesaving activity, have a disadvantage that they have a biodegradable structure which is easily oxidized and cause a loss of physiological activity due to rancidity and an unpleasant smell.

A variety of methods have been used to extract the ingredients of herbal medicines for skin cosmetics.

For example, Korean Patent Laid-Open Publication No. 2012-0018914 discloses that MCT oil is used as an extractive solvent of mung bean to extract a fat-soluble component of mung bean and then strengthen a specific component by fermentation with lactic acid bacteria, The present invention provides a cosmetic composition comprising mung bean extract as an active ingredient. Korean Patent Laid-Open Publication No. 2008-0104671 discloses a composition containing an extract obtained by fractionating a herbal plant extract comprising a gojija, a casualty, an omija, a tosaja, a bokbunja, a rhubarb, Discloses an anti-aging cosmetic composition. Korean Patent Laid-Open Publication No. 2009-0014320 discloses a fat composition containing a fat component made by fermentation of Omija, Kjijima, casualties, bokbunja, and soju, and a manufacturing method thereof. Korean Patent Laid-Open Publication No. 2008-0095555 also discloses a method of making oil by fermenting Goji berries and filling the soft capsules with fermented Goji berries oil.

In the above-mentioned technique, the fermentation and fermentation of the herbal ingredients by the fermentation process is effective as a method for obtaining an effective fat composition, but it is not easy to obtain economical production. In addition, the extraction method using a supercritical extractor is not easy to obtain a herbal cosmetic composition effectively because a maintenance ingredient effective in improving the skin's beauty is removed and only a part of the ingredient is extracted.

Korean Patent Publication No. 2012-0018914 Korean Patent Publication No. 2008-0104671 Korean Patent Publication No. 2009-0014320 Korean Patent Publication No. 2008-0095555

The inventors of the present invention have conducted intensive studies on a method of effectively extracting an effective ingredient contained in a herbal oil. As a result, when the oil is fermented using a specific strain, the content of the unsaturated fatty acid is remarkably increased. When the fermented oil is used as an extraction solvent It was confirmed that the useful component contained in the oriental herb can be effectively extracted owing to the similarity with the plant cell membrane, and the present invention was completed based on this finding.

The object of the present invention is to provide a cosmetic composition having an effect of skin barrier improvement, anti-allergy, intracellular oxidation inhibition and skin regeneration.

To this end, the present invention provides a cosmetic composition comprising as an active ingredient an extract of fermented oils of Omija, Gujia, Yeonja, Ginseng and Jijijiao.

According to the present invention, a fermented oil having a high content of unsaturated fatty acids, which are easily oxidized through fermentation, is used as an extraction solvent to extract a herbal extract, thereby effectively extracting useful components contained in herbal extracts. Skin barrier improvement, antiallergic, inhibition of intracellular oxidation, and skin regeneration, so that it is useful as a cosmetic composition for skin care.

1 is a graph showing the results of analysis of linoleic acid in fermented olive oil (a) and unfermented olive oil (b).
2 is a graph showing the results of evaluation of the ability of the composition of Example 1 and Comparative Examples 1 to 3 to proliferate to human dermal fibroblasts.
FIG. 3 is a graph showing the results of tests on the intracellular oxidative stress inhibitory ability of the compositions of Example 1 and Comparative Example 1. FIG.
4 is a graph showing the amount of beta-hexosaminidase released according to the composition treatment of Example 1 and Comparative Example 1. Fig.
FIG. 5 is a graph showing the results of quantitative analysis of fluorescence intensity after staining keratinocytes with FITC after application of the composition of Example 1 and Comparative Example 1. FIG.
FIG. 6 shows the results of measurement of keratinocytes by the fluorescence images according to the application of the composition of Example 1 and Comparative Example 1. FIG.

The composition according to the present invention contains, as an active ingredient, an extract obtained by extracting five kinds of oriental herbals with fermented oil, such as omija, gugija, liquor, ginseng and japonicum.

Schizandra chinensis Baillon) is the fruit of Schisandra chinensis tree schisandraceae (Schizabdraceae), an organic acid, sugar 1.5%, tanninjil, anthocyanin pigments, pectin, oil, resin and the like from about 8% saponin 5%, ascorbic acid 200 ~ 600 mg% ingredients . Seeds contain lignan compounds with antioxidative effects such as isocyanurines A, B, C, Sisandrol A, B andromycin B, C, D, F, G, Red), the content of lignan compounds is high. Omija has a strong taste and astringent effect, it regulates the sweat glands of the skin, protects, promotes the secretion of sperm, relieves thirst, and lowers blood sugar.

Lycium Chinense ) is the fruit of the Gujuga tree. The main ingredients of gugija contain carotene, vitamins B1, B2, vitamin C, linoleic acid, and beta-sitosterol. Gugija is known to have effects such as hematopoietic action, cholesterol lowering action, anti-hepatic action, blood pressure lowering, blood glucose lowering, growth promotion, and anticancer action.

Lotus ( Nelumbo nucifera ) is a fruit of lotus, its shape is elliptical or roundish, 1.2-1.8㎝ in length and 0.8-1.4cm in diameter. The surface is pale yellowish brown or reddish brown with a papilla on the center of one end and dark brown. The vagina is hard and does not split easily, the skin is thin and does not fall well. The taste is sweet and tender, and the quality is flat without any bias. Fruit seeds include methylcoridyldimethylcoclaurin, N-norcipherin, nelumbin, napalin, armepavin, rotofin, reincinin, isoleinecinin, nepheline and the like.

Ginseng is the fruit of ginseng, which is a bean-shaped fruit with red, red or yellow color depending on the type of ginseng. The skin and flesh parts contained 0.8% of pectin, 22.2% of hemicellulose, 46.3% of cellulose, and 24.0% of lignin, and the saponin of Rbc system and Rg system It is contained in an ideal balance and contains essential amino acids valine, alanine, phenylalanine, stannic acid, serine, histidine, peptide, corin adenosine, vitamin B1, B2, B12, folic acid, pantothenic acid and migneseum, calcium, , And minerals such as zinc, iron, copper, phosphorus, aluminum, vanadium, and germanium. Especially ginsenoside Re is known to be higher than roots.

Liguustrum lucidum refers to the fruit of Ligustrum japonicum in the ash tree and the dwarf myrtle ash tree (Ligustrum japonicum). Mica grows at the foot of a low mountain. It is gray with 3 ~ 5mfh high and has clear shrubs. Leaves are 3 ~ 10cm long and 2.5 ~ 4.5cm wide, facing like hairs and leather like, wide ovate or ovate long elliptical shape with sharp tip and no sawtooth. In July and August, white flowers bloom on the end of a new branch with a length of 5-12cm in length. The fruit is a long, round nucleus with a length of 8 ~ 10mm and ripen in purple to black color from October to November. In one room, the dried fruit is used as a medicine for herbal medicine. In the private sector, the leaf is boiled and applied to the boil. As a main component, rutinoside, oleanolic acid, quercetin, and urusholic acid are known.

Generally, the ingredients that are useful in skin cosmetics present in plants are present in the cells. In this plant cell, the cell membrane part is composed of a highly polar, highly hydrophobic component, which is a part of the cell in and out of which the plant cell comes in and goes out. The cell membrane also plays a role in preventing the outflow of the usable substance used for cosmetic purposes in cosmetics.

As a general hydrophobic solvent, it is difficult to extract an essential substance necessary for skin cosmetics in plants due to the structure of the cell membrane. However, the fermented oil has a similar composition to the cell membrane of the plant cell due to its unique composition and structure, which is higher in the content of unsaturated fatty acids than that of common oil. This structural similarity has the advantage of effectively obtaining ingredients useful for skin cosmetics inside the cells.

Generally, plant-derived hydrophobic oils containing a large amount of unsaturated fatty acids are known and used. However, as the content of unsaturated fatty acids increases, there is a possibility that the hydrophobic oils can be easily oxidized, which makes them unsuitable as a solvent. However, the fermented oil has an effective characteristic as a solvent because of its unique characteristic that it contains a large amount of unsaturated fatty acid and maintains oxidation stability. In addition, the fermented oil has a form that contains a large amount of unsaturated fatty acid and has a form of easy penetration into the skin because of the advantage that the molecular weight of the hydrophobic substance is reduced due to fermentation.

Therefore, by using such fermented oil, it is possible to effectively extract components useful for skin cosmetics contained in five oriental herbal extracts. The fermented oil extract thus extracted has a form that is easy to penetrate into the skin. Thus, the herbal cosmetic Thereby exhibiting a better skin-cosmetic effect.

An oil selected from the group consisting of soybean oil, corn oil, corn oil, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, camellia oil, castor oil, peanut oil and palm oil as an extracting solvent is mixed with Pseudozyma tsukubaensis Fermented oil obtained by fermentation is used.

Pseudozyma tsukubaensis is known to produce a biosurfactant (MEL-SY16) upon fermentation using an oil or the like as a carbon source (Korean Patent Registration No. 10-0532690).

In general, when the content of the unsaturated fatty acid increases, the stability of the acidic lung due to skin irritation or oxidation of the oil is lowered. However, in the present invention, the oil is fermented by using the Pseudosyma tsukubaensis , While the oxidation stability is improved at the same time.

Preferably, the fermentation oil can be obtained by adding the oil and Pseudozyma tsukubaensis to a fermentation vessel, fermenting the mixture at 25 to 40 ° C for 5-10 days, and then purifying the impurities.

The fermented oil extract of the present invention is obtained by adding the above-mentioned fermented oil to a mixture of omija, gugija, liquor, ginseng, At this time, the amount of fermentation oil used is 10 to 30 times the volume of the raw material, more preferably 20 times.

The extraction temperature and time may vary depending on the amount of the sample to be extracted, and it is preferable that the extraction is performed by heating at 60 to 100 ° C for 3 to 100 hours or at 60 to 70 ° C for 3 to 5 hours. In addition, the extract after extraction can be appropriately subjected to arbitrary operations within a range that does not impair the essence of the present invention such as filtration, centrifugation, extraction, purification, sterilization, etc., and the obtained extract is diluted, concentrated and dried It is possible.

In the present invention, the mixture of omija, gugija, liza, ginseng, and zoysiagrass can be mixed and controlled at appropriate ratios according to availability of raw materials, availability of raw materials, production costs, etc. of omija, gugija, Preferably 1: 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3, more preferably 1: 1: 1: : 1. ≪ / RTI >

In the cosmetic composition of the present invention, the fermented oil extract is preferably contained in an amount of 0.01 to 30% by weight based on the total weight of the composition. If the content is less than 0.01% by weight, no remarkable effect can be expected. If the content is more than 30% by weight, the increase of the content is not remarkable.

The cosmetic composition of the present invention may be prepared by any of the known formulations and may be in the form of lotions, nutritional lotions, nutritional creams, massage creams, essences, packs, solutions, suspensions, emulsions, pastes, gels, creams, lotions, It can be formulated into a form of a soap, an oil, a foundation, a wax or a spray. When it is applied as a cosmetic for hair, it can be formulated into a form such as shampoo, rinse, soap, hair cream, hair cream, hair gel, It is possible.

In addition, in the cosmetic composition of each formulation, components other than the fermented oil extract may be arbitrarily selected and formulated according to the formulation or purpose of use of other cosmetics. In addition, the compositions of each formulation may contain various bases and additives necessary for formulation of the formulation, and may contain a nonionic surfactant, a silicone polymer, an extender pigment, a flavoring, a preservative, a disinfectant , An oxidative stabilizer, an organic solvent, an ionic or nonionic thickener, a softening agent, an antioxidant, a free radical scavenger, an opacifying agent, a stabilizer, an emollient, a silicone, an a-hydroxy acid, a defoaming agent, , Insect repellents, perfumes, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basic or acidifying agents, or coloring agents.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples.

< Example  1 and Comparative Example  1 to 3>

The composition of each raw material of Example 1 and Comparative Examples 1 and 2 was added to a mixer as shown in Table 1 below, followed by extraction at 80 ° C for 3 hours, followed by cooling and filtration. Fermented olive oil was used as Comparative Example 3. The fermented olive oil was operated with SY16 [KCTC 8950P] as a microorganism. The working volume of the 5L fermenter was operated at 3 L, 500 rpm agitation, 1.0 vvm aeration at 30 ℃ and pH 4.0 (± 0.2) for 120 hours.

Item Experimental Example 1 Comparative Example 1 Comparative Example 2 Schisandra 100g 100g 100g Gugija 100g 100g 100g Journeyman 100g 100g 100g Lecturer 100g 100g 100g Ginseng 100g 100g 100g Fermentation
Olive oil 1Kg Olive oil 1Kg Purified water 1Kg

Experimental Example  1: Measurement of unsaturated fatty acid content

Fermented olive oil and non fermented olive oil were analyzed by HPLC and the results are shown in FIG. 1 Respectively.

[HPLC conditions]

Flow rate: 1.0 ml / min, Column temp. Solvent condition: Acetonitrile: Water = 50: 50 (0.1% Acetic acid): 35 ° C Column: Fortis C18 5μm, 4.6X150mm, Injection volume:

As shown in Fig. 1, it was confirmed that the fermented olive oil had significantly higher content of linoleic acid, which is one of unsaturated fatty acids, compared with olive oil.

In addition, fermented olive oil and unfermented olive oil were analyzed for free fatty acids according to KSM 0065 (1997). As a result, it was confirmed that the content of unsaturated fatty acid increased by 50 times or more in olive oil: 0.1294 mgKOH / g and fermented olive oil: 8.1022 mgKOH / g.

Experimental Example  2: Water loss ( TEWL ) Measure

The effect of skin moisture loss on the composition prepared above was investigated as follows.

In order to determine the amount of moisture loss of the skin at the time of one application, tape stripping was performed on the upper arm of the arm of 15 male and 15 female adults aged from 20 to 30 years who had no skin disease to weaken the skin barrier, After each test material was applied, it was applied once every 12 hours using Tewameter TM 300, a moisture evaporator of CK (Courage + Khazaka, Cologne, Germany) The evaporation amount of moisture was measured five times and an average value was obtained. The results are shown in Table 2 below.

division Example 1 (%) Comparative Example 1 (%) Comparative Example 2 (%) Comparative Example 3 (%) Water evaporation after 0 hours 100.0 100.0 100.0 100 Water evaporation after 12 hours 61.5 69.5 84.3 75.3 Water evaporation after 24 hours 44.3 56.6 73.1 59.9 Water evaporation after 36 hours 41.5 49.6 64.5 52.3 Water evaporation after 48 hours 40.1 47.6 56.1 50.7

As shown in Table 2, it can be seen that the composition according to the present invention has the best moisture evaporation inhibiting effect.

Experimental Example  3: Degree of appearance  evaluation

 In order to examine the degree of topicality of the composition according to the present invention, each of the compositions of Example 1 and Comparative Examples 1 to 3 prepared above was stored in an oven at 50 ° C for 3 months, sampled in a glass bottle, The phase stability was visually observed. Table 3 shows the evaluation results of the degree of topicality.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Degree of appearance
(50 캜, 3 months) ○ △ △ ○ Degree of appearance
(Daylight, 4 weeks) ○ △ △ ○ ○: stable, △: weak, X: severe

As shown in Table 3, it can be confirmed that the composition according to the present invention has a superior degree of appearance as compared with Example 1, Comparative Example 1, and Comparative Example 3. This suggests that the use of fermented olive oil, which is a fermented hydrophobic solvent, maintains the degree of appearance after extracting 5 herbal seeds even though the content of unsaturated fatty acid is large in the composition.

Experimental Example  4: Evaluation of cell proliferation effect

In order to confirm the improvement effect of the skin cosmetic composition of the present invention, the effect of the cell proliferation on the composition of Example 1 and Comparative Examples 1 to 3 was evaluated. The evaluation method is as follows.

1. Cell: Human dermal fibroblast neonatal (HDFn)

2. Course

Human dermal fibroblasts were seeded at a concentration of 5,000 cells / well in 96 wells and cultured for 24 hours to attach the cells to the plate.

The medium was removed and washed three times with PBS.

③ Sample treat media was diluted by concentration.

④ After culturing for 24 hours and 48 hours, the medium was removed.

⑤ 5 mg / ml MTT solution was diluted 10 times in DMEM medium and treated for 4 hours.

⑥ After the medium was removed, MTT formazan was dissolved by adding DMSO, and the absorbance was measured at 517 nm. The evaluation results are shown in Fig.

2 is a graph showing the results of evaluation of the ability of the composition of Example 1 and Comparative Examples 1 to 3 to proliferate to human dermal fibroblasts. 1, it was confirmed that the composition of Example 1 according to the present invention exhibited the best skin cell proliferation effect.

Experimental Example  5: Intracellular Oxidative stress Inhibition  exam

T In order to investigate the effect of the herbal extract according to the present invention on the removal of reactive oxygen species (ROS) from the cells, oxidation-sensitive dye 2 ', 7'-dichlorofluorescein diacetate (DCFH-DA) Experiments were performed as follows.

RAW 264.7 cells cultured in 96-well plates were labeled with 20 μM DCFH-DA in HBSS (Hanks balanced salt solution) for 30 min in the cancer. The cells were then treated with different concentrations of herbal extracts and incubated for an additional 1 hour. Cells were washed three times with PBS and then 500 μM H ₂ O ₂ was added. The intensity of the fluorescence signal emitted from 2 ', 7'-dichlorofluorescein (DCF) due to the oxidation of DCFH by cellular ROS was determined in a time-dependent manner using a GENios fluorescence microtiter reader (Tecan Austria GmbH, Austria) lambda excitation = 485 nm, lambda emission = 528 nm). After the increase in fluorescence intensity was maximized, each well was normalized to the number of cells using MTT cell viability assay. The treatment effects were plotted and compared with the fluorescence intensities of the control and blank groups. The results are shown in Fig.

As shown in FIG. 3, it was confirmed that the composition of Example 1, which was extracted with a hydrophobic solvent fermented before Comparative Example 1 extracted with a simple hydrophobic solvent (olive oil), suppressed intracellular active oxygen and was excellent in the ability to inhibit oxidative stress.

Experimental Example  6: ß- Hexosamidine  Release Inhibition  evaluation

In order to evaluate the effect of herbal extracts according to the present invention on the skin inflammation and allergy mitigating effects, the change in secretion of beta-hexosaminidase was measured.

Beta-hexosaminidase is an enzyme contained in mast cells. When mast cells are activated by an immune response, they are released along with histamine. Therefore, beta-hexosaminidase can be used as a degranulation index of mast cells.

RAB-2H3 cells, the mouse's basophilic leukocyte, were cultured in EMEM (Eagles Minimum Essential Medium) medium supplemented with 10% fetal bovine serum (FBS), 1% glutamine, 100 units / mL penicillin and 100 μg / mL streptomycin ) Was added and cultured in an incubator (37 ° C, 5% CO ₂ ). When grown to about 80% of the 100 mm dish bottom, the cells were treated with trypsin / versene and cultured for 18 hours in 2x10 ⁶ cells per well in a 24 well plate. After the medium was removed, the cells were reacted with 1 μg / ml of anti-DNP-IgE for 18 hours to sensitize the sample. The sample was pretreated for 1 hour and 10 minutes after the treatment with 10 μg / ml of DNP-BSA.

50 μl of the obtained supernatant and 50 μl of 5 mM p-nitrophenyl-N-acetyl-β-glucosamide dissolved in 0.2 M citrate buffer (pH 4.5) were incubated for 30 minutes in an incubator. To stop the reaction, 1 N HCl was added and the release of beta-hexosaminidase was measured at 405 nm. In order to measure the amount of total beta-hexosaminidase released, the cells were subjected to the same experiment as the supernatant obtained by hemolysis with 0.1% Triton X-100. The results are shown in Fig.

Referring to FIG. 4, it was confirmed that the composition of Example 1, which was extracted with a hydrophobic solvent fermented than that of Comparative Example 1 extracted with a simple hydrophobic solvent (olive oil), had better skin inflammation inhibitory effect.

Experimental Example  7: Evaluation of barrier improvement effect

In order to confirm the skin barrier improvement effect of the composition according to the present invention, the thus prepared composition of Example 1 and Comparative Example 1 was tested as follows.

1. Subjects: Three subjects aged 32 to 37 years (mean age 35 ± 2.6 years) with healthy skin

2. Test period: Immediately after tape stripping, 7 days after application

3. Evaluation method:

Subjects who were suitable for the purpose of the test were selected and skin barrier was artificially damaged by tape stripping method and then applied to the designated area of the skin twice a day for 7 days. As a measurement point, immediately after tape stripping, FITC was applied to the keratin extracted from the skin at 7 days of application, and the change of the keratinocyte extracted by staining was evaluated. The keratinocytes obtained at each time point were pretreated, and the morphological distribution of the neutrophil and the expression pattern of the keratinocyte protein were measured by a confocal microscope (using a confocal microscope and a dendritic device). The measured images were quantitatively analyzed for fluorescence intensity using Image analyzer pro and the results are shown in Table 4 below.

division After tape striping 7 days Example 1 11.00 1.36 Comparative Example 1 10.64 3.40

FIG. 5 is a graph showing the results of quantitative analysis of fluorescence intensity after staining keratinocytes with FITC after application of the composition of Example 1 and Comparative Example 1 (Example 1: red color, Comparative Example 1: blue color).

FIG. 6 shows the results of measurement of keratinocytes by the fluorescence images according to the application of the composition of Example 1 and Comparative Example 1. FIG.

Involucrin is a well-known epidermal differentiation marker that is involved in the differentiation process of keratinocytes. The more involucrin, the greater the differentiation of immature keratinocytes. Therefore, from the results of Table 4 and Figs. 5 to 6, it is shown that keratinocytes are more immature when they have strong fluorescence intensity, and herbal oil (Experimental Example 1) extracted with fermented oil is used before fermentation And showed a lower fluorescence intensity than the oil. Therefore, it can be said that it helps to improve the barrier of the skin by helping the normal keratinocyte differentiation in the herbal oil extracted with the fermented oil (Experimental Example 1).

Hereinafter, the formulations of the present invention are exemplified as a soft lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence and a pack, but the formulation of the cosmetic composition of the present invention is not limited thereto.

Formulation Example  1: Softening longevity ( skin )

As shown in the following Table 5, the softening water containing the fermented oil extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The fermented oil extract of Example 1 One glycerin 5 1,3-butylene glycol 3 Betaine One Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Sodium hyaruronate powder 0.05 ethanol 5 Octidodeces-16 0.2 Polyoxyethylene hardened castor oil 0.2 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Purified water Balance Sum 100

Formulation Example  2: Nourishing lotion (lotion)

As shown in Table 6 below, a nutritious lotion containing the fermented oil extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The fermented oil extract of Example 1 3 Glyceryl stearate SE 1.5 Cetearyl alcohol 1.0 Sheer butter 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil One Mineral oil 5 Squalane 3 Cyclomethicone 2 Dimethicone 0.8 Tocopheryl acetate 0.5 Carbomer 0.12 glycerin 5 1,3-butylene glycol 3 Sodium hyaruronate powder 0.05 Triethanolamine 0.12 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Distilled water Balance Sum 100

Formulation Example  3: Nourishing cream

As shown in Table 7 below, a nutritive cream containing the fermented oil extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The fermented oil extract of Example 1 3 Pro-type glycerin monostearate 2 Cetearyl alcohol 2 Stearic acid 1.5 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hydrogenated polyisobutene One Squalane 3 Mineral oil 5 Cyclomethicone 5 Dimethicone One Tocopheryl acetate 0.5 glycerin 5 Betaine 3 Triethanolamine One Xanthan gum 0.05 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Distilled water Balance Sum 100

Formulation Example  4 : Massage  cream

As shown in Table 8 below, a massage cream containing the fermented oil extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The fermented oil extract of Example 1 3 Pro-type glycerin monostearate 1.5 Cetearyl alcohol 1.5 Stearic acid One Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isoserate 5 Squalane 5 Mineral oil 35 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6 1,3-butylene glycol 3 Triethanolamine 0.3 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Distilled water Balance Sum 100

Formulation Example  5: essence

Essences containing the fermented oil extract of Example 1 were prepared according to a conventional method as shown in Table 9 below.

ingredient Content (% by weight) The fermented oil extract of Example 1 3 glycerin 6 Betaine 5 PEG 1500 2 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Hydrogenated lecithin 0.6 Hydroxyethylcellulose 0.1 Sodium hyaruronate powder 0.08 Carboxyvinyl polymer 0.2 Triethanolamine 0.2 Ceramide 0.2 Octyldodecanol 3 Squalane 3 Polysorbate 60 0.4 Glyceryl stearate SE 1.5 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Distilled water Balance Sum 100

Formulation Example  6: Pack

As shown in Table 10 below, a pack containing the fermented oil extract of Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) The fermented oil extract of Example 1 One Polyvinyl alcohol 15 Cellulose sword 0.15 glycerin 3 PEG 1500 2 Betaine 2 DL-Panthenol 0.4 Allantoin 0.1 Triethanolamine 0.2 Nicotinamide 0.5 ethanol 6 PEG 40 hardened castor oil 0.3 incense Suitable amount antiseptic Suitable amount Pigment Suitable amount Distilled water Balance Sum 100

Claims (7)

A cosmetic composition comprising a fermented oil extract of Schizandra chinensis, Rhizoctonia sp. The fermented milk according to claim 1, wherein the fermented oil is selected from the group consisting of soybean oil, corn oil, corn oil, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, camellia oil, castor oil, Characterized in that it is obtained by fermenting with Pseudozyma tsukubaensis . The cosmetic composition according to claim 2, wherein the fermented oil is obtained by adding Pseudozyma tsukubaensis to the oil and fermenting at 25 to 40 ° C for 5 to 10 days. The fermented oil extract according to claim 1, wherein the fermented oil extract is obtained by immersing a mixture of Omija, Gujia, Zhao, ginseng, and herbarium with fermentation oil at 4-30 DEG C for 0.5-30 days, Which is characterized in that it is extracted by heating for a period of time The cosmetic composition according to claim 4, wherein the mixture is a mixture of omija, gugija, lime, ginseng, and isophyllum at a ratio of 1-3: 1-3: 1-3: 3-3: 1-3. . The cosmetic composition according to claim 1, wherein the content of the fermented oil extract is 0.1 to 30% by weight based on the total weight of the cosmetic composition. The composition of claim 1, wherein the composition is a solution, suspension, emulsion, paste, gel, cream,
Lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, packs,
From the group consisting of essences, emulsion foundation, wax foundation and spray
Wherein the composition has one selected formulation.

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