KR20120091911A - Fermented extract of oriental medicine plant complex, method for manufacturing the same and cosmetic compositions comprising the oriental medicine plant complex - Google Patents

Abstract

PURPOSE: An oriental medicinal complex extract fermentation and a cosmetic composition containing the same are provided to remove reactive oxygen in the skin and to ensure antioxidation effect. CONSTITUTION: A method for preparing an oriental medicinal complex extract fermentation comprises: a step of mixing Gigantic Angelica, Rehmannia glutinosa, Cnidium officinale, and Paeonia lactiflora Pall. var. hortensis Makino and extracting with water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, or hexane to obtain extract; a step of filtering and freeze-drying the extract; a step of adding yeast to the concentrated resultant and fermenting at 4-40 Deg. C. for 30 days to 12 months. A cosmetic composition contains 0.00002-30 wt% of the fermentation. The cosmetic composition is manufactured in the form of skin softener, lotion, essence, nutrition cream, body cream, baby lotion, or baby cream.

Description

Fermented extract of Oriental medicine plant complex, method for manufacturing the same and cosmetic compositions comprising the oriental medicine plant complex}

The present invention relates to a cosmetic composition comprising an extract of fermented Oriental medicine, Angelica, Cheongung, and Peony, as an active ingredient, a method of manufacturing the same, and a method of producing the same as an active ingredient, and more particularly in water and organic solvents. It relates to a herbal medicinal herb composite extract fermentation applied fermentation technology to the selected herbal medicines extracted with one or more selected, the preparation method and the herbal medicinal herb composite extract fermentation.

Aging refers to the degenerative changes in living organisms that occur with aging. The most prominent causes of aging in recent years are stress, polluted air and water, convenience foods, and ultraviolet light.

In particular, three emerging skin aging theories are endocrine (Endocrine theory), free radical theory (Toxic waste theory), free radical theory (Toxic waste theory).

In recent years, the most popular theory of free radical (Free radical theory) is that free radicals (active oxygen) damage DNA, proteins, and lipids, causing aging.

In general, anti-aging functional cosmetics include antioxidants for the purpose of preventing or delaying the oxidation and decomposition of fats and oils used as cosmetic softener raw materials by free radicals caused by dissolved oxygen and ultraviolet rays dissolved in air or water. I'm making it.

Among the antioxidants developed and used so far, synthetic antioxidants include tert-butyl hydroxyltoluene (BHT) and tert-butylhydroxyanisol (BHA). Tocopherol (α-tocopherol), vitamin C, vitamin E, carotenoids (carotenoids), flavonoids (flavonoids), tannins and the like. In addition, antioxidant enzymes include Superoxide dismutase (SOD).

Recently, as oxidative stress has been proved to be an important cause of various diseases including aging, the possibility of anti-aging of antioxidants having free radical scavenging activity and treatment of diseases has been greatly highlighted. However, until now, natural and synthetic antioxidants have been developed and used as medicines or research reagents, but due to various problems in toxicity and in vivo, they are limited in use as medicines for the treatment of aging due to oxidative stress and various diseases. It has Therefore, the development of new antioxidants that can solve the problems of the existing antioxidants is required.

In order to solve the problems as described above, the present invention provides a herbal extract extract fermentation and a method for producing the same, which is safe for the skin and excellent in the antioxidant effect among natural effective substances.

The present invention provides a cosmetic composition comprising the herbal medicine complex extract fermentation.

Herbal medicine complex extract fermentation according to the embodiments of the present invention, extracts Angelica, gihwang, cheongung and peony with one or more selected from water and organic solvents and yeast ( Sacchromyces cerevisiae ) can be used for fermentation.

The Angelica, Chihwang, Cheongung and Peony may be mixed in a 1: 1: 1: 1 weight ratio (w / w / w / w), respectively.

Herbal medicine composite extract fermentation method according to the embodiments of the present invention is a mixture of Angelica, Chihwang, Cheongung and Peony mixed with water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane Obtaining an extract by extracting with one or more solvents selected from the group consisting of, filtering the extract with one or more selected from filter cloth, filter paper, and a vacuum filter, and freeze drying the yeast ( Sacchromyces) in the concentrated product. cerevisiae ) may comprise the step of fermentation.

The Angelica, Chihwang, Cheongung and Peony may be mixed in a 1: 1: 1: 1 weight ratio (w / w / w / w), respectively.

The extraction may be to extract shaking for 6 to 24 hours at 10 ~ 80 ℃.

The fermentation may be carried out for 30 days to 12 months at 4 ~ 40 ℃.

The freeze drying may include a step of freeze drying for 36 to 48 hours from -20 ℃ to 30 ℃ step by step to obtain a dry powder.

Cosmetic composition according to the embodiments of the present invention may include the herbal medicine complex extract fermentation.

The herbal herbal extract extract fermentation may be included in 0.00002 ~ 30% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be formulated as a flexible cosmetics, nourishing cosmetics, essence, nutrition cream, body cream, baby lotion or baby cream.

According to embodiments of the present invention, by applying the fermentation technique to the herbal medicine complex extract containing Angelica, Chihwang, Cheongung and Peony can increase the content of the active ingredient exhibiting an antioxidant effect. Therefore, by using the herbal herbal extract extract fermentation according to the embodiments of the present invention it is possible to effectively protect the skin cells by removing the harmful free radicals in the skin.

1 is a graph showing the antioxidant effect through DPPH scavenging ability.
2 is a graph showing the antioxidant effect through the SOD-like activity.
Figure 3 is a graph showing the antioxidant effect through the hydrogen peroxide (Hydrogen peroxide) scavenging ability.
Figure 4 is a graph of the cytotoxicity of Example 1.
5 is a graph confirming the antioxidant effect through the UV spectrum measurement.
Figure 6 is a graph of HPLC analysis of the indicators of Angelica, Chihwang, Cheongung and Peony.
Figure 7 is a graph of the primary analysis of the indicator material of the herbal medicine complex extract by HPLC.
8 is a graph of the first analysis of the indicator of Example 1 by HPLC.
9 is a graph of secondary analysis of the indicator material of the herbal medicine complex extract by HPLC.
10 is a graph obtained by analyzing the indicator material of Example 1 by HPLC.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples. The objects and features of the present invention can be easily understood through the following examples and experimental examples. The present invention is not limited to the examples and experimental examples described below, but may be embodied in other forms. Examples and experimental examples introduced below are provided to sufficiently convey the technical spirit of the present invention to those skilled in the art. Therefore, the present invention should not be limited by the following examples and experimental examples.

In the present invention, the step of extracting at least one selected from water and an organic solvent after grinding the Angelica, Chihwang, Cheongung and Peony; And fermenting the herbal medicines of Angelica vulgaris, Chihwang, Cheongung and Peony extracted into yeasts; and then, preparing herbal extracts of fermented medicinal herbs through the yeast, 3) electron donating ability experiment using DPPH, superoxide dismutase dismutase (SOD) -like activity test and hydrogen peroxide (Hydrogen peroxide) scavenging test was confirmed the antioxidant effect.

Angelica used in the present invention (當歸, Scientific name: Gigantic Angelica ) is the root of Angelica gigas, an herb belonging to the Apiaceae family. Vitamin B12, folic acid (Folic acid), and iron (鐵 分) contains hematopoietic action to improve blood flow and hematocrit (hematocrit) of the blood (hematocrit) increases. It also contains vitamin E, which acts on vitamin E deficiency. Angelica essential oil (精油) to expand blood vessels to lower blood pressure and promote blood flow to the peripheral blood vessels by expanding blood circulation to improve peripheral circulation disorders.

Chihwang (地 黄, scientific name: Rehmannia used in the present invention) glutinosa ) is the root of perennial herbaceous plants. It contains iron to help hematopoietic, and vitamin A to prevent skin keratinization, soften skin and soften keratinocytes. Among the components, catalpol (Catalpol) has a significant hypoglycemic effect on hyperglycemia (高 血糖) and diuretic effect.

Cheongung used in the present invention (川芎, scientific name: Cnidium officinale ) is a perennial herb from erectus and uses roots or stems. Ferullic acid, which is a component, has analgesic, cough and smooth muscle relaxation.

Peony used in the present invention (芍藥, scientific name: Paeonia lactiflora Pall . there is . hortensis Makino ) is a perennial herb from the peony and is a horticultural species called hawthorn and uses its roots. Many kinds of sugars, mucus, organic acids and trace minerals are contained a lot, the malnutrition of the limit (盜汗) to reduce the convergence effect. In particular, antibacterial activity against dysentery bacillus, Staphylococcus aureus, Pseudomonas aeruginosa, E. coli bacterium, strong immune function and anti-inflammatory action.

In the herbal medicinal herb extract extract fermentation according to the embodiments of the present invention can be mixed in a variety of weight ratios, Angelica, jihwang, cheongung and peony, the angelica, jihwang, cheongung and peony are 1: 1: 1: 1 weight ratio, respectively It may be desirable to be (w / w / w / w).

As the extraction solvent used in the step of extracting the Angelica, gihwang, cheongung and peony, one or more selected from the group consisting of water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane can be used. Can be. At this time, the extraction temperature is preferably 10 to 80 ℃, it can be extracted for 6 hours to 24 hours. If the extraction temperature and extraction time is out of the extraction efficiency may decrease or change of components may occur.

Herbal medicine according to the embodiments of the present invention can be prepared according to the conventional method of manufacturing herbal medicine extract. Specifically, it may be prepared by a cold extraction method, a hot extraction method or a heat extraction method, it may be used a conventional extraction device or ultrasonic grinding extractor.

The prepared extract can then be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter cloth or filter paper or a reduced pressure filter. The concentration may be concentrated under reduced pressure using, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In addition, the herbal medicinal herb complex extract can completely remove the moisture through the concentration and lyophilization of the extract obtained, the herbal medicinal herb complex extract completely removed the moisture can be used in powder form.

In the fermentation step, fermentation (醱 酵) in a broad sense refers to the process of obtaining a substance useful to humans using microorganisms or fungi, and in a narrow sense refers to a sugar decomposition process to obtain energy without using oxygen. Most organisms need oxygen for breathing, and oxygen breathing gives them the energy they need to work. On the other hand, creatures living in an oxygen-deficient environment, such as deep below the ground or at the bottom of a lake, can adapt to the environment and get energy without oxygen. This breathing is called anoxic breathing. Fermentation is one such oxygen-free breath. During fermentation, organic matter is decomposed to form another organic matter.

Fermentation process according to an embodiment of the present invention is preferably fermented 30 days to 1 year, preferably 6 months to 1 year so that sufficient fermentation can occur between 4 ~ 40 ℃. If it is less than 4 ℃, the growth of useful microorganisms is not well, and if it exceeds 40 ℃ can grow too much of the microorganisms may affect the performance of the combined herbal medicine.

Herbal composite extract fermentation product according to the present invention is not particularly limited to its use, and may be used, for example, additives of health foods, cosmetic compositions and pharmaceutical compositions. In particular, it can be used as a cosmetic composition with excellent antioxidant effect, the content of which is 0.00002 to 30% by weight, preferably 0.005 to 0.02% by weight relative to the total weight of the composition. If it is less than 0.00002% by weight it is difficult to obtain the expected antioxidant effect, if it is more than 30% by weight it is difficult to expect additional increase in efficacy.

< Example >

1. Herbal Medicine Complex Extract Preparation

Angelica, jihwang, cheongung and peony used in the present invention used a domestic herbal medicine certified GAP. Good Agricultural Practices (GAP) refers to hazard prevention rules in the production and handling processes that producers and managers must follow to ensure safe and hygienic livestock products to consumers. To minimize the risks to the environment and to provide consumers with safe food, the management and management of chemicals, heavy metals and microorganisms during the cultivation, harvesting, post-harvesting, storage, and storage of agricultural and livestock products. It is a system.

200 g of distilled water was added to 200 g of a mixture of dried Angelica 50g, Ghwang 50g, Cheongung 50g, and Peony 50g, which are GAP certified Korean herbal medicines, boiled for 10 hours in a hot water extractor with a cooling condenser, and cooled to room temperature. The medicine was filtered through a mesh filter cloth and filtered using an 11 μm paper filter. A portion was taken and filtered using a 0.45 μm membrane filter in a reduced pressure filter and then lyophilized to obtain a final extract.

2. Medicinal Herb Extract Complex Fermentation

Fermentation was carried out with six strains of 200 ml each of the herbal extracts extracted by hot water. Six strains are known as Sacchromyces cerevisiae , Example 1), Lactobacillus ( Latobacillus bulgaricus , Example 2), Phellinus fungus baumii , Example 3), Bacillus subtilis , Example 4), Paecilomyces japonica , Example 5), Ganoderma lucidum ( Ganoderma) lucidum , example 6). The inoculation amount of the strain was 5% on average and incubated at 37 ° C. The fermented extract was filtered with an 11 μm paper filter, filtered using a 0.45 μm membrane filter in a reduced pressure filter, and lyophilized to obtain a final extract fermentation product.

Experimental Example  1. Antioxidant Effect Experiment

1) Electron donating ability experiment using DPPH

In order to determine the presence or absence of antioxidant activity for the compositions according to the present invention, 5 × 10-4M of DPPH (1,1-to 100 μl of solution of each extract (Herbal medicinal herb complex extracts, Examples 1 to 6) 100 µl of diphenyl-2-picryl-hydrazyl) was added thereto, stirred for 1 minute, and then reacted in a 25 ° C. incubator for 30 minutes. Absorbance was measured at 540 nm using a microplate counter. L-ascorbic acid (100µg / ml) was used as the experimental control, and the electron donating ability was expressed as the absorbance decrease rate of the addition and no addition of the sample solution. The results of measuring the DPPH electron donating ability of the extract are shown in Table 1 and FIG.

sample Concentration (µg / ml) DPPH Clearance (%) L-ascorbic acid 100 79 Herbal Medicine Complex Extract 1000 14 100 6 10 3 Example 1 1000 38 100 8 10 0 Example 2 1000 13 100 -One 10 5 Example 3 1000 13 100 9 10 5 Example 4 1000 14 100 0 10 -2 Example 5 1000 15 100 3 10 -6 Example 6 1000 18 100 -3 10 One

Compared with the herbal medicinal herb complex extract not fermented as shown in Table 1 and Figure 1 and the fermented herbal medicinal herb extract fermentation (Examples 1 to 6), DPPH scavenging ability is about 40 in Example 1 fermented with yeast It was the highest as% and showed excellent antioxidant effect.

2) Superoxide dismutase (SOD) -like activity test

SOD is a bioactive enzyme present in the organs and blood of humans and animals that removes harmful oxygen. It is reported that SOD-like active substances protect phytochemicals by inhibiting the reactivity of superoxide radicals by playing a similar role to SOD (Murakami et al., 2003).

SOD-like activity was measured for the samples of the herbal medicine complex extract as follows. 200 μl of WST working solution in the SOD assay kit (SST assay kit-WST) was added to 20 μl of the sample solution for each concentration of 100 ppm of each extract, followed by the enzyme working solution. 20 μl was added and mixed. The mixture was reacted for 20 minutes in a 37 ° C. incubator, and the absorbance was measured at 450 nm using a microplate counter. Superoxide dismutase (100 units, Sigma) was used as an experimental control, and superoxide dismutase-like activity was indicated by the absorbance reduction rate of the sample solution added and the non-added sample. The results of measuring SOD-like activity are shown in Table 2 and FIG.

Sample (100 µg / ml) SOD
(100units) Herbal Medicine
Complex extract Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 SOD-like activity (%) 98 19 64 28 8 27 34 36

As shown in Table 2 and Figure 2, the SOD-like activity of Example 1 was the highest among the herbal herbal extract extract fermentation. Therefore, it can be seen that there is an excellent antioxidant effect in Example 1.

3) Hydrogen peroxide scavenging ability test

Herbal medicine complex extract and hydrogen peroxide (Hydrogen peroxide) scavenging ability measurement for Examples 1 to 6 was carried out as follows. 20 μl of 10 mM Hydrogen peroxide and 100 μl of 0.1 M phosphate buffer (pH 5) were added to 20 μl of the sample solution for each concentration of 100 ppm, respectively, and reacted for 5 minutes in a 37 ° C. incubator. 30 μl of 1.25 mM ABTS and 30 μl of 1 U / ml peroxidase were added, and reacted for 10 minutes in a 37 ° C. incubator, and the absorbance was measured at 405 nm using a microplate counter. Ascorbic acid (100 ppm, Sigma) was used as an experimental control. Hydrogen peroxide scavenging activity measurement results are shown in Table 3 and FIG. 3.

Sample (100 µg / ml) Ascorbic acid Herbal Medicine
Complex extract Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 H ₂ O ₂ scavenging capacity (%) 4 21 44 17 23 16 30 18

As shown in Table 3 and Figure 3 when the herbal herbal extracts were fermented with yeast (Example 1), hydrogen peroxide (Hydrogen peroxide) scavenging ability was the highest. Therefore, it can be seen that the best antioxidant effect in Example 1.

On the basis of the results of the antioxidant effect test, it was confirmed that the herbal extracts were the most effective when the herbal extracts fermented with yeast (Example 1). Therefore, the toxicity experiment on the skin epithelial cells of Example 1 was carried out as follows.

Experimental Example  2. Cytotoxicity Test

In order to examine the cytotoxicity of the herbal extract extract fermentation (Example 1), human fibroblast cells (Human fibroblast cells) at a concentration of 5x104 cells / ml 200μl in 96well plate and 37 ℃, CO2 incubator Incubated for 1 day at. At this time, the medium was used as IMDM (lscove's Modified Eagle Medium, Gibco) containing 10% bovine serum and 1% antibiotics. After incubation was replaced with IMDM medium without bovine serum and antibiotics, Example 1 was added for each concentration, and then incubated for 45 hours under the same culture conditions. After incubation, 5 mg / ml MTT (Thiazolyl Blue Tetrazolium Bromide) reagent was added and incubated for 3 hours under the same conditions. Thereafter, the culture medium was removed, 150 μl of DMSO (Dimetyl sulfoxide) was added thereto, and then shaken for 10 minutes. The result was measured at 570 nm of a micro plate reader. DMSO is held blank, and the results are shown in Table 4 and FIG. 4.

Concentration (µg / ml) blank 1000 750 500 250 100 50 10 5 One Cell proliferation (%) 100 60 37 74 85 95 109 111 103 109

As shown in Table 4 and Figure 4 it was confirmed that Example 1 does not exhibit toxicity at a concentration of 250 μg / ml or less.

Experimental Example 3 . Herbal Medicine Complex Extract Fermentation product UV Spectrum  Measure

In the antioxidant experiments, it can be seen that Example 1 shows the best effect among the six extract fermentations. In order to support these results, the wavelengths of the six herbal medicine complex extracts and six herbal medicine complex extract fermentations (Examples 1 to 6) were measured by UV spectrum measurement. The UV Spectrum instrument used for the measurement was a Shimazdu UV-1650PC, a UV-Visible spectrophotometer with a sample concentration of 0.25% (solvent is ultrapure water). UV Spectrum measurement results, as shown in Figure 5, when comparing the respective examples, it can be seen that the absorbance of Example 1 is the strongest.

Experimental Example  4. Assay

1) Indicator substance of herbal medicine

The following are the indicators of herbal medicine used in this study. Angelica is nodakenin (Nodakenin, Wako, 148-08331, 5mg), Sulfur is 5 -HM (5-Hydroxymethyl-2-furaldehyde, Aldrich, H40807, 1g), Cheongung is chlorogenic acid (Chlorogenic acid, Sigma, C3878, 250mg) , Peony was purchased for analysis experiments (Peoniflorin, Wako, 164-21731, 10mg).

2) Preparation of Analytical Indicators

Analytical indicators were 5 mg nodakenin, 10 mg 5- HMF, 10 mg chlorogenic acid, and 10 mg peoniflorin and dissolved uniformly in 100 ml of 80% methanol. Thereafter, 2.2 ml of nodakenin, 2.0 ml of 5-HMF, 0.35 ml of chlorogenic acid, and 3.0 ml of peoniflorin were each added to a 10 ml volumetric flask, and the remainder mass-up with 80% methanol. Homogenized. Part of the complete indicator solution was taken and filtered with a 0.45 μm membrane syringe filter and then placed in an assay vial for analysis.

3) Preparation of analytical extract and extract fermentation

The herbal extracts lyophilized and 0.33 g each in Example 1 were weighed into a 100 ml volumetric flask and mass-up with 80% methanol. After homogenizing between the extract and the solvent, only a part of the completed solution was taken and filtered with a 0.45 μm membrane syringe filter, and then put into an assay vial.

4) HPLC Assay

The high-performance liquid chromatographic column used for extract analysis was Capcell pak C18, 5㎛ 4.6 × 250㎜ (Shiseido). The column temperature is room temperature, the flow rate is 1.0 ml / min, and the detector is 230 nm. Mobile phase A was used as 0.1% Phosphoric acid in Water, mobile phase B was used as 0.1% Phosphoric acid in Acetonitrile, and solvent conditions were as follows. Mobile phase B gradually increased to 0-25% for 30 minutes and maintained 25% by 33 minutes. After changing the mobile phase B from 25 minutes to 25-100% from 33 minutes to 35 minutes, the mobile phase B was continuously flowed from 100 minutes to 35 minutes to 50 minutes.

5) HPLC analysis result

HPLC analysis of the indicator, extract and fermented extract are as follows.

Figure 6 is the result of measuring the indicator substances of the four herbal medicines according to the conditions. Nodakenin, an indicator of donkey, was found in 30.5 minutes, 5- HMF (5-Hydroxymethyl-2-furaldehyde) in 12.16 minutes, and Chlorogenic acid, a marker of hormonal, 19.83 minutes. In Peony, Paeoniflorin was detected at 25.69 minutes, respectively. On the basis of this result, the herbal extracts were compared with the changes in the indicator substance of Example 1 and HPLC analysis was performed twice.

The results of the first analysis are shown in FIGS. 7, 8, and 5.

division Herbal Medicine Complex Extract
(mg / 0.33g) Example 1
(mg / 0.33g) Comparison of contents
(Based on herbal medicine complex extract) Chlorogenic acid (bow) 43 60 39.5% ↑ Peonyflorin (Pea Blossom) 403 659 63.5% ↑

As shown in Table 5 and FIGS. 7 and 8, the content of the indicator material of Example 1 was increased by 39.5% and 63.5% in case of Cheongung and Peony.

The secondary analysis results are shown in FIGS. 9, 10, and 6.

division Herbal Medicine Complex Extract
(mg / 0.33g) Example 1
(mg / 0.33g) Comparison of contents
(Based on herbal medicine complex extract) Chlorogenic acid (bow) 41 59 43.9% ↑ Peonyflorin (Pea Blossom) 448 663 48.0% ↑

As shown in Table 6 and FIG. 9 and FIG. 10, the content of the indicator material of Example 1 was increased by 43.9% and 48.0%, respectively.

The following is each cosmetic formulation containing Example 1 having the best antioxidant effect among the herbal complex mixed extract fermentation. Removes harmful free radicals in the skin, protects skin cells, shows an example of a cosmetic having a superior anti-aging and antioxidant effect.

Prescription Example  1. Flexible Cosmetics

ingredient Content (% by weight) Example 1 0.01 Polyoxyethylene Cured Castor Oil 0.5 Glycine 3.0 Dipotassium glycylizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example  2. Nutritional Cosmetics

ingredient Content (% by weight) Example 1 0.01 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.3 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example  3. Essence

ingredient Content (% by weight) Example 1 0.01 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyaluronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl cellulose 0.2 ethanol 3.0 Antioxidant 0.3 Triethanolamine 0.1 Polyoxyethylene Cured Castor Oil 1.0 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example  4. Nutrition Cream

ingredient Content (% by weight) Example 1 0.01 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example  5. Body cream

ingredient Content (% by weight) Example 1 0.01 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyaluronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 antiseptic Suitable amount Purified water Balance

Prescription Example  6. Baby Lotion

ingredient Content (% by weight) Example 1 0.01 glycerin 5.0 Panthenol 4.0 Ceramide 0.1 Phytosing 0.1 Cetearyl Alcohol 0.5 Phospholipids 1.0 Stearic acid 0.5 Camellia oil 7.0 Shea Butter 1.0 Sodium Copolyacrylate (5% dispersion) 10.0 Compound preservative 0.5 Purified water Balance

Prescription Example  7. Baby Cream

ingredient Content (% by weight) Example 1 0.01 glycerin 5.0 Panthenol 4.0 Ceramide 0.5 Phytosing 0.5 Cetearyl Alcohol 1.0 Phospholipids 3.0 Stearic acid 1.5 Camellia oil 16.0 Shea Butter 2.0 Sodium Copolyacrylate (5% dispersion) 15.0 Compound preservative 0.5 Purified water Balance

So far we looked at the specific examples and experimental examples for the present invention. Those skilled in the art will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments and experimental examples should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (10)

Angelica root extract, Rehmannia glutinosa, Cnidium officinale Makino and Paeonia to at least one selected from water and an organic solvent, and yeast (Sacchromyces cerevisiae ) fermented herbal extracts fermented with herbal medicine. The method of claim 1,
Herbal medicine, Chihwang, Cheonggung and Peony are herbal medicine complex extract fermentation, characterized in that mixed in a 1: 1: 1: 1 weight ratio (w / w / w / w). Mixing Angelica gigas, Chihwang, Cheongung and Peony and extracting with water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane with one or more solvents selected from the group consisting of extracts;
Filtering the extract with at least one selected from a filter cloth, a filter paper, and a vacuum filter, and lyophilizing the extract; And
Sacchromyces in the concentrated product cerevisiae ) method of manufacturing herbal herbal extract extract fermentation comprising the step of fermentation. The method of claim 3, wherein
The Angelica, gihwang, cheongung, and peony are 1: 1: 1: 1 weight ratio (w / w / w / w) manufacturing method of the herbal medicine complex extract, characterized in that mixed. The method of claim 3, wherein
The extraction is a method for producing a herbal medicine complex extract, characterized in that the extract for shaking for 6 to 24 hours at 10 ~ 80 ℃. The method of claim 3, wherein
The fermentation is a method of producing a herbal medicine complex extract, characterized in that carried out for 30 days to 12 months at 4 ~ 40 ℃. The method of claim 3, wherein
The freeze-drying is a method for producing herbal medicine complex extract fermentation comprising the step of obtaining a dry powder by freeze drying for 36 to 48 hours from -20 ℃ to 30 ℃ step by step. Cosmetic composition containing the herbal medicine complex extract fermentation of Claim 1 or 2. The method of claim 8,
The herbal medicine complex extract fermentation is a cosmetic composition, characterized in that contained in 0.00002 ~ 30% by weight based on the total weight of the cosmetic composition. The method of claim 8,
The cosmetic composition is a cosmetic composition, characterized in that formulated as a flexible cosmetics, nourishing cosmetics, essence, nutrition cream, body cream, baby lotion or baby cream.

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