KR20110131783A - Cosmetic composition comprising the extract of heat-processed panax ginseng showing skin anti-aging activity - Google Patents

Abstract

PURPOSE: A composition containing processed extract of Panax plants is provided to protect keratinocytes irritated by UV and to ensure anti-aging. CONSTITUTION: A cosmetic composition for anti-aging contains processed Panax ginseng extract as an active ingredient containing more ginsenoside Rg3, Rg5, and Rk1 content than ginsenoside Rb1, Rb2, Rc, and Rd content. The processed Panax ginseng extract is prepared by slicing the Panax ginsengs and heating the Panax ginsengs in an autoclave at 70°C-150°C for one hour to 24 hours. The Panax ginsengs are Panax ginseng C.A. Meyer, Panax quinquefolium, Panax notoginseng, Panax japonicum, Panax trifolium, or Panax pseudoginseng.

Description

Cosmetic composition for anti-aging containing ginseng extract as an active ingredient {Cosmetic composition comprising the extract of heat-processed panax ginseng showing skin anti-aging activity}

The present invention relates to a cosmetic composition for preventing skin aging comprising a processed ginseng extract as an active ingredient.

Reference 1 Fisher, GJ, Wang, ZQ, Datta, S., Varani, J., Kang, S., Voorhees, JJ,. Pathophysiology of premature skin aging induced by ultraviolet light. N. Engl . J. med . 337 , pp. 1419-1428, 1997.

Yamamoto, Y., Role of active oxygen species and antioxidants in photoaging. J. Dermatol . Sci . 27 , pp. S1-S4, 2001.

[3] Sung Mok Beak, Yong Soo Lee, Jung-Ae Kim, NADPH oxidase and cyclooxygenase mediate the ultraviolet B-induced generation of reactive oxygen species and activation of nuclear factor-kB in HaCaT human keratinocytes. J. Biochem . 86 , pp. 425-429, 2004.

Masaki, H., Atsumi, T., Sakurai, H.,, Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem . Biophys . Res . Commun . 206 , pp. 474-479, 1995.

Document 5 Karin, M., Liu, Z.-G., Zandi, E., AP-1 function and regulation. Curr. Opin . Cell . Biol . 9 , pp. 240-246, 1997.

[6] Barber, LA, Spandau, DF, Rathman, SC, Murphy, RC, Johnson, CA, Kelly, SW, Hurwitz, SA, Travers, JB,, Expression of the platelet-activating receptor results in enhanced ultraviolet B radiation -induced apoptosis in a human epidermal cell line. J. Biol . chem . 273 , pp. 18891-18897, 1998.

Literature 7 Lee KS, Lee WS, Suh SI, Kim SP, Lee SR, Ryoo YW, Kim B C. Melatonin reduces ultraviolet-B induced cell damages and polyamine levels in human skin fibroblasts in culture. Exp Mol Med . 35 (4) : pp.263-8, 2003.

Millis, AJT, Hoyle, M., McCue, HM, Martini, H.,, Differential expression of metalloproteinase and tissue inhibitor of metalloproteinase genes in aged human fibroblasts. Exp . Cell . Res . 201 , pp. 373-379, 1992.

9, Cha, HJ, Bae, SK, Lee, HY, Lee, OH, sato, H., Seiki, M., Park, BC, kim, KW,, Anti-invasive activity of ursolic acid correlates with the reduced expression of matrix metalloproteinase-9 (MMP-9) in HT1080 human fibrosarcoma cells. Cancer Res . 56 , pp. 2281-2284, 1996.

10. Brenneisen, P., Wenk, J., Klotz. LO, Wlaschek, M., Briviba, K., Krieg, T., Sies, H., Scharffetter-Kochanek, K., Central role of ferrous / ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloproteinase (MMP) -1) and stromelysin-1 (MMP-3) mRNA levels in cultured human dermal fibroblasts. J. Biol . Chem . 273 , pp. 5279-5287, 1998.

[11] Yong-Sam Keum, Kwang-Kyun Park, Jong-Min Lee, Kyung-soo Chun., Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. J. Cancer Letters . 150 , pp. 41-48, 2000.

12. Young C. Kim, So R. Kim, George J. Markelonis, Tae H. Oh., Ginsenosides Rb1 and Rg3 Protect Cultured Rat Cortical Cells From Glutamate-Induced Neurodegeneration. J. Neuroscience Res . 53 , pp. 426-432, 1998.

Keun Young Jung, Dong seon Kim, Platelet Activating Factor Antagonist Activity of Ginsenosides. Biol . Pharm . Bull . 21 . PP. 79-80, 1998.

[Document 14] Korean Patent 10-0192678 Processed Ginseng Product with Enhanced Drug Effect, 1999

Global environmental changes, such as the destruction of the ozone layer, make skin more exposed to ultraviolet light. Ultraviolet rays (UV) can be classified into UVA, UVB, and UVC according to their wavelength. Among them, UVB mainly affects the epidermis and causes skin damage (1). Keratinocytes on the skin surface are most affected by direct exposure to UVB. In the skin exposed to ultraviolet rays, reactive oxygen species (ROS) are produced through various pathways, and excessively generated free radicals cause oxidative stress on the skin. Reactive oxygen species (ROS) ultimately lead to skin aging and skin inflammation such as erythema and edema, and severe skin cancer (2). ROS include superoxide anion, hydrogen peroxide, and hydroxyl radical, which are produced by NADPH cytochrome P450 reductase, NADPH oxidase, and lipoxygenase during intracellular oxygen respiration (3-4).

However, free radicals increased by UV rays damage cellular tissues and cause inflammation and may cause skin aging. At the same time, UV may be activated by the activation of intracellular signaling systems such as MAPK (mitogen, activated protein kinase) and PKC (protein kinase C) and transcription factors such as AP-1 and NF-κB. Influence (5). TGF _β , PGE ₂ and α-MSH released from keratinocytes are increased by UV, which activates melanocytes to increase melanin biosynthesis (6-7). In addition, TNFα and IL-1, which are free from keratinocytes by UV, act on dermal fibroblasts, which are dermal dermal cells, resulting in MMP-1 (martrix metalloproteinase-1) and TIMP-1 (tissue). It alters the biosynthesis of inhibitors of metalloproteinase-1 and procollagen (8-9). In other words, by increasing the release of protease such as MMP-1, collagen (collagen) is degraded, collagen synthesis is inhibited to cause skin aging (10).

Ginseng Panax ginseng ) is a processed ginseng that has dramatically increased the contents of Rg ₃ , Rg ₅ , and Rk ₁ , which are highly effective by treating at high temperature and high pressure. The efficacy of ginseng as an anticancer action (11), brain function improving action (12), platelet aggregation ability blocking action (13) and the like is reported.

The purpose of this study was to evaluate the effects of UV on the injury of skin keratinocytes by extract of ginseng, a processed ginseng. For this, LDC and NO glass were measured by UV treatment of human keratinocytes, HaCaT, and genes of COX-2, Bcl-2, Bax, iNOS, COX-2, TNFα, NF-κB, c-fos, and c-jun The effect on expression was evaluated.

In order to achieve the above object, the present invention provides a composition for preventing skin aging containing the processed Panax ginseng extract as an active ingredient.

Therefore, the present invention is for preventing skin aging containing a processed Panax ginseng extract, which has a higher sum of the contents of ginsenosides Rg3, Rg5, Rk1 than the sum of the contents of ginsenosides Rb1, Rb2, Rc, and Rd as an active ingredient. It provides a cosmetic composition.

Specifically, Panax ginseng extract disclosed herein has a higher sum of the contents of ginsenosides Rg3, Rg5, Rk1 than the sum of the contents of ginsenosides Rb1, Rb2, Rc, Rd, preferably ginsenosides (Rg3). The ratio of + Rg5) / (Rc + Rd + Rb₁ + Rb2) is 1.0 or more, more preferably 3.0 or more, even more preferably 5.0 or more.

Specifically, the processed Panax ginseng extract of the present invention is an extract obtained by cutting the Panax genus plant into a autoclave and putting it in an autoclave at 70 ° C. to 150 ° C. for about 1 hour to 24 hours under about 2 to 30 atmospheres. It is done.

More specifically, the processed Panax ginseng extract of the present invention, after cutting the Panax genus plants in the autoclave and heated at 70 ℃ to 150 ℃, about 2 to 30 atm for about 1 hour to 24 hours, the sample About 1 to 20 times the weight ratio (w / w) of water, C ₁ to C ₄ lower alcohols, or a mixed solvent thereof, by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, or heating extraction After extraction, the filtrate is characterized in that the ginseng processed extract obtained through a process comprising the step of filtration and concentration under reduced pressure and drying.

In addition, “panax ginseng” as defined herein may be commonly used ginseng, preferably ginseng (Panax ginseng CA Meyer), Panax quinquefolium, Panax notoginseng, Panax japonicum, Panax ginseng, such as Panax trifolium or Himalayan ginseng.

The present invention provides a composition for preventing skin aging containing the processed ginseng extract obtained by the above manufacturing process as an active ingredient.

Therefore, the present invention The dried ginseng is chopped and placed in an autoclave, at 70 ° C. to 150 ° C., preferably at 100 ° C. to 130 ° C., at about 10 to 30 atm, preferably at 13 to 18 atm, for about 1 to 24 hours, preferably After heating for 3 to 5 hours, a weight ratio (w / w) of water of about 1 to 20 times, preferably about 3 to 10 times the weight of the sample, C ₁ to C ₄ lower alcohols or their With a mixed solvent, preferably a mixed solvent of water and ethanol, extracted by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, heating extraction, preferably reflux cooling extraction, and the filtrate is filtered. It provides a cosmetic composition for preventing skin aging containing ginseng processed extract obtained through a process comprising the step of concentration under reduced pressure and drying as an active ingredient.

The cosmetic composition for preventing skin aging of the processed ginseng extract may be used in an amount of 0.001 to 90% by weight, preferably 0.01 to 10% by weight, based on the total weight of the composition.

In addition, the cosmetic composition comprising the processed ginseng extract of the present invention includes a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polysaccharides, sphingolipids and seaweed extract. The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, D-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, DL-alphatocopherol acetate, DL-alpha tocopherolvitamin E. DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenylethyl nicotinic acid) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be cosmetically blended. Preferably, seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like, and calginane, arginic acid, sodium arginate and argin purified from these seaweed extracts. Potassium ginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Other components that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta lysitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearate isostearic acid , Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, citric acid Triether, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate Dioctyl sebacate, stearic acid cholesterol, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, phosphate oleate , 12-stealoyl hydroxystearate isocetyl, 12-stealoyl hydroxystearate stearic acid, 12-s Allo one hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

As silicone fats and oils, polymethine silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methyl steoxysiloxane copolymer, and alkyl modified silicone Oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based oil include perfluoropolyether and the like.

As animal or vegetable fats and oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil , Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum, Marker demia nut oil, Meadow home oil, Egg yolk oil, Ujima oil, Mink oil, Orange rape oil, Jojoba oil, Canderry wax, Carnabas wax And animal or plant fats and oils, such as liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As a water-soluble low molecular moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone- carboxylate titanium, glycerin, propylene glycol, 1, 3- butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned. Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters. As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose , Water soluble chitin, chitosan, dextrin and the like. Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Examples of nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE glycerin fatty acid esters, and POE alkyls. Ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE POP (polyoxyethylene polyoxypropylene) copolymer, POE POP organic ether, polyether modified silicone, lauric acid alkanolamide, alkylamine Oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

Examples of cationic surfactants include alkyltriethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylmenzyl ammonium, behenyltrimethylammonium, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and composites thereof; Polyamide, Polyester, Polypropylene, Polystyrene, Polyurethane, Vinyl Resin, Urea Resin, Phenol Resin, Fluoro Resin, Silicon Resin, Acrylic Resin, Melamine Resin, Epoxy Resin, Polycarbonate Resin, Divinylbenzene Styrene Copolymer, Silk Organic pigments such as powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentel salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cyanic acid octyl, daparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-ρ- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

 Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, Is formulated at 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors and Carrier.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , Fatty acid esters of 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carriers such as water, ethanol or propylene glycol, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The composition containing the ginseng extract of the present invention inhibits LDH release due to injury of HaCaT, a skin keratinocyte line stimulated by UV, inhibits NO production, expression of iNOS, a ROS-related factor by UV, and c-jun, a transcription factor. It shows the effect of inhibiting the expression of c-fos and NF-kB. It protects keratinocytes stimulated by UV and controls skin damage related factors by UV to inhibit skin damage and thus be used as a cosmetic composition for preventing skin aging. Can be.

1 is a view of the effect on the production of Nitrite by UVB of ginseng extract,
2 is a diagram showing the effect on the LDH glass by UV irradiation of the ginseng extract,
3 is a diagram showing the effect on the expression of COX-2 gene by UVB of ginseng extract,
4 is a diagram showing the effect on the expression of TNFα gene by UVB of ginseng extract,
Figure 5 is a diagram of the effect on the expression of c-jun gene by UVB of ginseng extract,
6 is a diagram showing the effect on the expression of c-fos gene by UVB of ginseng extract,
Figure 7 is a view of the effect on the expression of iNOS gene by UVB of ginseng extract.

Hereinafter, the present invention will be described in detail by the following.

However, the following Examples, Reference Examples and Experimental Examples are merely illustrative of the present invention, but the content of the present invention is not limited thereto.

Example 1 Preparation of Ginseng Extract

The dried ginseng (Gumsan ginseng market) was chopped and heated using steam at 120 ° C. and 15 atm for 4 hours. 2 L of 80% ethanol was added to 1 kg of processed ginseng (hereinafter referred to as “ginseng”), and the extract obtained by reflux extraction for 4 hours or more in a water bath was concentrated under reduced pressure, and then freeze-dried to obtain about 300 g of an extract powder. In the experiment, the ginseng extract (hereinafter referred to as “SG”) was dissolved in a medium using DMSO (Sigma D-2650), and used after passing a filter paper having a pore size of 0.45 μm. (Rg3 + Rg5 + Rk1) / (Rb1 + Rb2 + Rc + Rd) = 6.1 of the ginseng extract used in the experiment.

Example 2. Analysis of Saponin Content of Sea Ginseng Extract

The ginseng saponin component contained in the processed ginseng prepared according to the present invention obtained in Example 1 was analyzed by the following method.

Four 40 ml stainless steel vessels were added with 5 g of white ginseng and 5 ml of water, respectively, and then sealed and heated at 110 ° C. for 2 hours, 120 ° C. for 2 hours and 3 hours, and 130 ° C. for 2 hours. 5 g of each of the processed ginseng and the commercially produced white ginseng and red ginseng were taken, extracted three times with 100 ml of methanol, concentrated, suspended in water, and extracted three times with 100 ml of ether. The remaining larvae were extracted three times with 100 ml of butanol, and then the butanol fraction was concentrated. The obtained concentrate was dissolved in methanol, and HPLC (column: LiChrosorb NH2, mobile phase: CH₃CN / H₂O / i-PrOH = 80/5/15) was extracted. → 80/20/15, detector: analyzed by ELSD (Evaporative light scattering detector). The measured results are as described in Table 1 below.

As can be seen from the results shown in Table 1, the processed ginseng obtained by heat treatment according to the present invention has a significant increase in the content of Rg3 and Rg5 components, which are ginseng saponins, which are hardly or never present in fresh ginseng, white ginseng, and red ginseng. It shows excellent efficacy.

According to the results as described above, in order to more specifically confirm the change in the content of saponin components, in particular ginsenosides Rg3 and Rg5 according to the change in the heating temperature for ginseng, heating is 100 ℃, 110 ℃, 120 ℃, 130 ℃, Rg3 and Rg5 contents of ginseng obtained by 2 hours at 150 ° C., 160 ° C., 180 ° C. and 200 ° C. were measured and compared with those in untreated ginseng (water ginseng). The results are shown in Table 2 below.

As seen above, the content of ginsenosides such as Rg3 and Rg5 is significantly increased in the case of processed ginseng heated at 120 to 180 ° C as in the present invention, compared to the case of untreated ginseng or red ginseng (100 ° C heating). It can be seen that. (Rg3 + Rg5 + Rk1) / (Rb1 + Rb2 + Rc + Rd) = 6.1 of the ginseng extract used in the experiment.

Reference Example  1. Cell

HaCaT keratinocyte, a human skin keratinocyte line used in the experiment, It was used for sale from Fusenig (German Cancer Research Center, DKFZ, Heidelberg, Germany).

Reference Example 2. Statistics Processing

Student's t-test was used to test the significance of each result. It was determined that p <0.05 was significant.

Experimental Example 1. Effect on skin keratinocyte activity

1-1. HaCaT  Cell culture

HaCaT keratinocyte, a human dermal keratinocyte line, It was used for sale from Fusenig (German Cancer Research Center, DKFZ, Heidelberg, Germany). Cells DMEM (Dulbecco's Modified Eagle Medium, Gibco BRL Co. USA) 10% FBS (GIBCO Inc. 14-501F), 10% penicillin-streptomycin (100 unit / ㎖, 100 ug / ㎖) CO 2 in a culture medium is added to the Incubator (SANYO, JAPAN) was incubated under 37 ℃, 5% CO ₂ , 95% air conditions. For cell division, 0.25% trypsin-EDTA (GIBCO's 25200) was used, followed by incubating overnight with 96 wells, 1x10 ⁵ / well in 24 wells, and 1x10 ⁶ / ml in 6 wells. It was.

1-2. UV irradiation and drug treatment

UV irradiation was performed using a UVATEC (Sherman Oaks, CA, USA) irradiator, and UVB lamp was irradiated with UV light having a wavelength of 290-320 nm. Light quantity was measured using an IL1700 radiometer (International Light Inc., MA, USA). The drug was incubated in advance by adding concentrations (2, 10, 50 µg / ml) for 1 hour before UV irradiation. In order to irradiate ultraviolet rays, DMEM medium (WelGENE LM001-05) was removed, washed twice with PBS (welGENE LB001-02), and then irradiated with UV rays having a UVB wavelength of 30 mJ / cm ² . After irradiating with UV light, and then washed once with PBS, a new DMEM medium containing the drug by concentration was added and cultured for a predetermined time, and then used for the experiment.

1-3. Nitrite ( Nitrite ) Generation  Measure

In order to measure the oxidative stress of the skin keratinocytes by UV, the amount of nitrite generated after UV irradiation to HaCaT cells was measured.

After incubation in the same manner as the LDL method described in Experimental Example 1-4 below, the culture supernatant was taken from the experimental group. Serial dilutions of the NaNO ₂ solution were made and adjusted to a final concentration of 0.05 μM. First, an equal amount of grease reagent solution (Griess reagent G4410) was added to the cell culture solution and the NaNO ₂ dilution solution, and the absorbance was measured at 540 nm after being allowed to stand at room temperature for 15 minutes.

As a result, as shown in Figure 1, the amount of nitrite produced in the cell at the time of UV irradiation increased. In addition, when SG was treated, the production of nitrite was suppressed compared to the UV-irradiated control group, thereby suppressing oxidative stress caused by UV.

1-4. LDH Viable  Measure

Cells were preincubated for 24 hours using 96 well plates and then pretreated with media containing the drug for 1 hour. Cells were added 1 × 10 ⁴ per well. After removing the culture medium, 50 μl of PBS (phosphate buffered saline) (WelGENE LB001-02) was added to each well, and after 30 mJ / cm ² of UVB was irradiated to the cells, the PBS was removed and the drug per well was 200 μl of DMEM medium included was added. After 24 hours, the culture medium was taken to measure lactate dehydrogenase (LDH) activity as an indicator of cell damage. LDH activity was quantified using Sigma's LDH kit (Sigma S 500), and absorbance was measured using an ELISA Reader (Molecular Device Emax).

As a result, as shown in Figure 2, the release of LDH from the cells during the UV irradiation increased. In addition, when treated with ginseng (SG), the release of LDH was suppressed compared to the control group irradiated with UV, showing the effect of inhibiting dermal cell injury by UV.

Experimental Example  2. Impact on Gene Expression

In order to determine the effect on the expression of MMP-1, Procollagen, c-fos, c-jun, iNOS gene of the ginseng extract of Example 1 was performed as follows.

2-1. gun RNA  detach

HaCaT cells cultured in the manner described in Experiment 1-1 were treated with 1 ml TRIzol reagent (Invitrogen, Cat. No. 15596018) to isolate total RNA. 200 μl of chloroform (Sigma-Aldrich, Cat.No. 288306): isoamylalcohol (Sigma-Aldrich, Cat.No. 320021) (24: 1) was added to the isolated RNA solution, followed by vigorous mixing and centrifugation at 14000 rpm. 500ul was separated. 0.5 ml isopropyl alcohol was added thereto to precipitate RNA overnight at minus 20 ° C., followed by centrifugation at 1400 rpm for 20 minutes. The supernatant was discarded, washed with 70% ethanol (Sigma-Aldrich, Cat. No. 459844) and dried naturally. After RNA was dissolved in RNAase removal water (RNase-free Water, Sigma-Aldrich, Cat. No. 95289), RNase-free DNase (Promega, Cat.No. M6101) was added and stored at -70 ° C.

2-2. cDNA  Produce

1 μl of oligo dT (Promega, Cat.No. C1101) (concentration 100 pmol) in 1 ul of total RNA solution (containing 1 ug RNA) isolated from the control and test groups, and RNAase removal water (Promega, Cat.No. M6101). ) 3 ul was added, mixed carefully, and incubated at 65 ^o C for 10 minutes. Leave for 5 minutes at 4 ^o C to anneal the primers, then reverse transcriptase buffer, dNTP (Promega, Cat.No. U1515) (2.5 mM each), RNase inhibitor (Promega, Cat. No. N2611), DTT (100 nM), and Reverse transcriptase (M-MLV 200 U / ul) (Promega, Cat. No. M5301) were added and then mixed very carefully. Thereafter, the mixture was incubated at 42 ^° C. for 90 minutes, and then used after treatment at 95 ° C. for 5 minutes.

2-3 RT - PCR

Oligo (dT) primer (Promega, Cat.No. C1101), reaction buffer; 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl ₂ , 10 mM DTT, pH 8.3) (Promega, Cat.No. M1705), 1 mM dNTP (Promega, Cat.No. U1515), and 200 units of M-MLV-RT (Moloney murine leukemia virus reverse transcriptase) (Promega, Cat.No. M1705) were treated to RNA isolated. CDNA was synthesized by performing reverse transcription. PCR was performed by adding cDNA and 1.25 units of Taq polymerase (Promega, Cat.No. M8295) to a mixture containing 10X PCR buffer, 0.2 mM dNTPs, 2 pmole sense, and anti-sense primer in 25 μl total volume. . The primers used were made to order at Bioneer Korea.

The sense primer of COX-2 was “TTC AAA TGA GAT TGT GGG AAA AT” and the antisense was “AGA TCA TCT CTG CCT GAG TAT CTT”, and the sense primer of iNOS was “CGG TGC TGT ATT TCC TTA CGA GGC GAA GAA GG ”and antisense used“ GGT GCT GTC TGT TAG GAG GTC CAA GTA AAG GGC ”. The sense primer of TNFα was “TGC ACC ACA GTT TAA ACC CA” and the antisense was “GAC TCC TTC AGG TGC TCA GG”. NF-κB2 sense primers were used as “TCC ACC TTT AGG TTG CCC TG” and antisense was used as “TCT GCT CTC GTC ATG TCA CC” and c-Jun sense primers were “GGA TCA AGG CGG AGA GGA AG” and antisense The GCG TTA GCA TGA GTT GGC AC was used, the sense primer of c-Fos was used as GGA GAA TCC GAA GGA AAG G, and the antisense was GCT TGG GCT CAG GGT CAT TG. The sense primer of Bcl-2 is “ACT CTG CTC AGT TTC GCC CT” and the antisense is “TTG TGG CTC AGA TAG GCA C”. The sense primer of Bcl-xL is “ATG TCT CAG AGC AAC CGG” and antisense is “TCT TTC CGA CTG AAG AGT G” was used.

GAPDH was used as the control and sense primer was “CAGC CTC GTC CCG TAG ACA AA” and antisense was “CAC GAC ATA CTC AGC ACC GGC”.

 PCR conditions were 94 ° C. 4 min, 30 cycles [94 ° C. (20 sec), 54 ° C. (20 sec), 72 ° C. (30 sec), 72 ° C. 10 min (Perkin Elmer, USA). Electrophoresis was performed on 2% agarose gel. Bands from the electrophoresis results were obtained using a density analysis program, Gel-Pro analyzer 3.1 (Media Cybernetics.USA).

2-4. Real time RT - PCR

In each optical tube (MicroAmp® Optical 96-Well Reaction Plate with Barcode and Optical Adhesive Films, Applied Biosystems, Cat.No. 4314320) 2.5 μl of triple SybrGreen Mix (Sigma-Aldrich, Cat.No. S9430), above 1 μl of synthesized cDNA, 1 μl of 10 pmol / μl primer pair mix, 2.5 μl of dNTP 2 μl, 10 × Tag polymerase buffer 2.5 μl, Tag polymerase 0.3 μl and 14.7 μl H ₂ O, respectively, 95 ^o C 5 min 1 cycle, Amplification was performed at 95 ^° C 30sec, 45 ^° C 30sec, 72 ^° C 60sec 40 cycles, and 95 ^° C 20 min 1 cycle. After PCR, remove the tube, measure PCR specificity on 3% agarose gel using 5ul of reaction solution, and use the ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Cat.No. 4349157) in real time. (real time) PCR results were analyzed.

As a result, as shown in Figure 3, the production of COX-2 mRNA produced in the cell during UV irradiation increased. In addition, when SG was treated, COX-2 gene expression was suppressed compared to UV irradiated control, and it was shown to inhibit PGE ₂ production by UV.

As shown in FIG. 4, TNFα increased the production of mRNA of TNFα produced in cells upon UV irradiation. In addition, SG treatment was found to be able to indirectly inhibit the fibiroblast action by UV by reducing the increased TNFα gene expression in the UV irradiation control.

As shown in FIG. 5, c-jun increased the production of c-jun mRNA generated in cells upon UV irradiation. In addition, SG treatment was found to be able to indirectly inhibit the fibroblast action by UV by reducing the increased c-jun gene expression in the UV irradiation control.

As shown in Figure 6, c-fos increased the production of c-fos mRNA produced in the cell upon UV irradiation. In addition, SG treatment was found to reduce the increased c-fos gene expression in the UV irradiated control, thereby inhibiting fibroblast action by UV.

In addition, iNOS increased the production of mRNA of iNOS produced in cells upon UV irradiation, as shown in FIG. 7. In addition, SG treatment was shown to reduce the iNOS gene expression increased in the UV irradiated control, inhibiting the fibroblast action by UV.

Therefore, the ginseng extract inhibited skin keratinocyte injury by UV, which was found to have an excellent effect on inhibiting skin damage by controlling the expression of apoptosis-related factors and regulators increased by UV.

Recombinant protein Primer sequence COX-2 Sense: SEQ ID NO: 1 5'-TTC AAA TGA GAT TGT GGG AAA AT-3 Antisense: SEQ ID NO: 2 5'-AGA TCA TCT CTG CCT GAG TAT CTT-3 ' iNOS Sense: SEQ ID NO: 3 5'-CGG TGC TGT ATT TCC TTA CGA GGC GAA GAA GG-3 ' Antisense: SEQ ID NO: 4 5'-GGT GCT GTC TGT TAG GAG GTC CAA GTA AAG GGC-3 ' TNFα Sense: SEQ ID NO: 5 5'-TGC ACC ACA GTT TAA ACC CA-3 ' Antisense: SEQ ID NO: 6 5'-GAC TCC TTC AGG TGC TCA GG-3 ' NF-κB2 Sense: SEQ ID NO: 7 5'-TCC ACC TTT AGG TTG CCC TG-3 ' Antisense: SEQ ID NO: 8 5'-TCT GCT CTC GTC ATG TCA CC-3 ' c-Jun Sense: SEQ ID NO: 9 5'-GGA TCA AGG CGG AGA GGA AG-3 ' Antisense: SEQ ID NO: 10 5'-GCG TTA GCA TGA GTT GGC AC-3 ' c-Fos Sense: SEQ ID NO: 11 5'-GGA GAA TCC GAA GGA AAG G-3 ' Antisense: SEQ ID NO: 12 5'-GCT TGG GCT CAG GGT CAT TG-3 ' Bcl-2 Sense: SEQ ID NO: 13 5'-ACT CTG CTC AGT TTC GCC CT-3 ' Antisense: SEQ ID NO: 14 5'-TTG TGG CTC AGA TAG GCA C-3 ' Bcl-xL Sense: SEQ ID NO: 15 5'-ATG TCT CAG AGC AAC CGG-3 ' Antisense: SEQ ID NO: 16 5'-TCT TTC CGA CTG AAG AGT G-3 ' GAPDH Sense: SEQ ID NO: 17 5'-CAG CCT CGT CCC GTA GAC AAA-3 ' Antisense: SEQ ID NO: 18 5'-CAC GAC ATA CTC AGC ACC GGC-3 '

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation example  1. Preparation of Hydrophilic Ointment

Ginseng Extract 9.00%

White Vaseline 36.00%

Stearyl Alcohol 31.00%

Trace amounts of ether (or methyl) ρ-oxybenzoate

Propylene Glycol 19.00%

Sodium Lauryl Sulfate 2.40%

Trace amount of propyl ρ-oxybenzoate

Total 100.00%

Formulation example  2. Flexible Cosmetics skin Manufacturing

Ginseng Extract 2.00%

Glycerin 5.00%

Triethanolamine 0.50%

Tocopheryl Acetate 1.00%

Liquid Paraffin 5.00%

Squalane 5.00%

Macadamia Nut Oil 15.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

Carboxyl Vinyl Polymer 0.20%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation example  3. Milk croissant  Produce

Ginseng Extract 5.00%

1,3-butylene glycol 4.00%

Glycerin 3.00%

Oleyl Alcohol 0.05%

Polysorbate20 1.00%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation example  4. Manufacturing of Nutritional Cream

Ginseng Extract 2.00%

Glycerin 5.00%

Triethanolamine 0.50%

Tocopheryl Acetate 1.00%

Udon paraffin 5.00%

Squalane 5.00%

Macadamia Nut Oil 15.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

Carboxyl Vinyl Polymer 0.20%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation example  5. Massage  Manufacture of cream

Ginseng Extract 2.00%

Vaseline 5.00%

Liquid Paraffin 30.00%

Beeswax 3.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

1,3-butylene glycol 5.00%

Glycerin 5.00%

Preservative traces

Trace amount

Purified water level

Total 100.00%

<110> GINSENG SCIENCE, INC. <120> Cosmetic composition comprising the extract of heat-processed          panax ginseng showing skin anti-aging activity <130> DIF / 2010-04-0002 / KH <160> 18 <170> KopatentIn 1.71 <210> 1 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> COX-2 <400> 1 Thr Thr Cys Ala Ala Ala Thr Gly Ala Gly Ala Thr Thr Gly Thr Gly   1 5 10 15 Gly Gly Ala Ala Ala Ala Thr              20 <210> 2 <211> 24 <212> PRT <213> Artificial Sequence <220> <223> COX-2 <400> 2 Ala Gly Ala Thr Cys Ala Thr Cys Thr Cys Thr Gly Cys Cys Thr Gly   1 5 10 15 Ala Gly Thr Ala Thr Cys Thr Thr              20 <210> 3 <211> 32 <212> PRT <213> Artificial Sequence <220> <223> iNOS <400> 3 Cys Gly Gly Thr Gly Cys Thr Gly Thr Ala Thr Thr Thr Cys Cys Thr   1 5 10 15 Thr Ala Cys Gly Ala Gly Gly Cys Gly Ala Ala Gly Ala Ala Gly Gly              20 25 30 <210> 4 <211> 33 <212> PRT <213> Artificial Sequence <220> <223> iNOS <400> 4 Gly Gly Thr Gly Cys Thr Gly Thr Cys Thr Gly Thr Thr Ala Gly Gly   1 5 10 15 Ala Gly Gly Thr Cys Cys Ala Ala Gly Thr Ala Ala Ala Gly Gly Gly              20 25 30 Cys     <210> 5 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> TNFalpha <400> 5 Thr Gly Cys Ala Cys Cys Ala Cys Ala Gly Thr Thr Thr Ala Ala Ala   1 5 10 15 Cys Cys Cys Ala              20 <210> 6 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> TNFalpha <400> 6 Gly Ala Cys Thr Cys Cys Thr Thr Cys Ala Gly Gly Thr Gly Cys Thr   1 5 10 15 Cys Ala Gly Gly              20 <210> 7 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> NF-kB2 <400> 7 Thr Cys Cys Ala Cys Cys Thr Thr Thr Ala Gly Gly Thr Thr Gly Cys   1 5 10 15 Cys Cys Thr Gly              20 <210> 8 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> NF-kB2 <400> 8 Thr Cys Thr Gly Cys Thr Cys Thr Cys Gly Thr Cys Ala Thr Gly Thr   1 5 10 15 Cys Ala Cys Cys              20 <210> 9 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> c-Jun <400> 9 Gly Gly Ala Thr Cys Ala Ala Gly Gly Cys Gly Gly Ala Gly Ala Gly   1 5 10 15 Gly Ala Ala Gly              20 <210> 10 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> c-Jun <400> 10 Gly Cys Gly Thr Thr Ala Gly Cys Ala Thr Gly Ala Gly Thr Thr Gly   1 5 10 15 Gly Cys Ala Cys              20 <210> 11 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> c-Fos <400> 11 Gly Gly Ala Gly Ala Ala Thr Cys Cys Gly Ala Ala Gly Gly Ala Ala   1 5 10 15 Ala Gly Gly             <210> 12 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> c-Fos <400> 12 Gly Cys Thr Thr Gly Gly Gly Cys Thr Cys Ala Gly Gly Gly Thr Cys   1 5 10 15 Ala Thr Thr Gly              20 <210> 13 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> Bcl-2 <400> 13 Ala Cys Thr Cys Thr Gly Cys Thr Cys Ala Gly Thr Thr Thr Cys Gly   1 5 10 15 Cys Cys Cys Thr              20 <210> 14 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Bcl-2 <400> 14 Thr Thr Gly Thr Gly Gly Cys Thr Cys Ala Gly Ala Thr Ala Gly Gly   1 5 10 15 Cys ala cys             <210> 15 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> Bcl-xL <400> 15 Ala Thr Gly Thr Cys Thr Cys Ala Gly Ala Gly Cys Ala Ala Cys Cys   1 5 10 15 Gly gly         <210> 16 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Bcl-xL <400> 16 Thr Cys Thr Thr Thr Cys Cys Gly Ala Cys Thr Gly Ala Ala Gly Ala   1 5 10 15 Gly Thr Gly             <210> 17 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> GAPDH <400> 17 Cys Ala Gly Cys Cys Thr Cys Gly Thr Cys Cys Cys Gly Thr Ala Gly   1 5 10 15 Ala Cys Ala Ala Ala              20 <210> 18 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> GAPDH <400> 18 Cys Ala Cys Gly Ala Cys Ala Thr Ala Cys Thr Cys Ala Gly Cys Ala   1 5 10 15 Cys Cys Gly Gly Cys              20

Claims (6)

A cosmetic composition for preventing skin aging containing, as an active ingredient, a processed Panax ginseng extract having a higher sum of contents of ginsenosides Rg3, Rg5, and Rk1 than the sum of contents of ginsenosides Rb1, Rb2, Rc, and Rd. The method of claim 1,
The processed Panax ginseng extract is a cosmetic composition, characterized in that the extract obtained by cutting the panax genus plants into autoclaved sterilizer at 70 ℃ to 150 ℃, heated for about 1 to 24 hours under about 2 to 30 atm. The method of claim 1,
The processed Panax ginseng extract is cut into the Panax genus plants and put in a autoclave sterilized at 70 ℃ to 150 ℃, heated for about 1 to 24 hours under about 2 to 30 atm, about 1 to 20 times the weight of the sample After extracting by pear weight ratio (w / w) of water, C ₁ to C ₄ lower alcohol or a mixed solvent thereof by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, or heat extraction, the filtrate was extracted. Cosmetic composition, characterized in that the ginseng processed extract obtained through a process comprising the step of filtration and concentrated under reduced pressure. According to claim 1, wherein the genus Panax ginseng (Panax ginseng CA Meyer), Panax quinquefolium, Panax notoginseng, Panax japonicum, Panax trifolium or Himalaya ginseng (Panax pseudoginseng) Cosmetic composition). According to claim 1, wherein the extract is a cosmetic composition, characterized in that contained in 0.001 to 90% by weight relative to the total weight of the composition. The method of claim 1, wherein the composition is a formulation selected from the group consisting of a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence Cosmetic composition.

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