KR20090093363A - Cosmetic composition comprising the extract of Polygonum multiflorum showing skin whitening and anti-aging activity - Google Patents

Abstract

A composition containing Polygonum multiflorum extract as an active ingredient is provided to suppress the expression of tyrosinase, TRP-1, TRP-2, PKA, PKC, and MITF and ensure excellent skin whitening and anti-aging effect. A composition for skin whitening and anti-aging comprises Polygonum multiflorum extract as an active ingredient. The Polygonum multiflorum extract is obtained using water, low alcohol of 1-4 carbon atoms or their mixture solvent, hexane, propyleneglycol, acetic acid ethyl, buthyleneglycol, ether, or chloroform. The cosmetic composition is used in the form of lotion, skin, nutrition cream,, massage cream, essence, pack, cleansing, soap, and treatment.

Description

Cosmetic composition for skin whitening and skin aging containing dripping sewage extract as an active ingredient {Cosmetic composition comprising the extract of Polygonum multiflorum showing skin whitening and anti-aging activity}

The present invention relates to a skin whitening and skin anti-aging cosmetic composition containing a drop sewage extract as an active ingredient.

[Summary 1] Park, Soo Nam et al., Application of plant-derived components to whitening cosmetics (functional cosmetics), Seoul National University of Technology, 52, pp205-220, 2001

2 Tong X et al., Mol . Cell Biol . , 27 (1), pp283-96, 2007

3 Romero GC et al., J. Clin . Invest . , 99 (4), pp635-642, 1997

[Document 4] Son Ae-Ryang, Seung-Jae Lee, Journal of Korean Aesthetic Society, 6 (1), pp239-254, 2000

[Reference 5] 山 孝 一 良 and hearing, VJ.メ ラ ニ ソ 産生 の 制 御 因子. Fragnance Journal , 6, pp24-28, 1990

Jackson IJ et al., EMBO J. , 11, pp519, 1992

Lee J. et al., Biochem . Pharmacol . , 74 (7), pp 960-968, 2007

Document 8 Huang SC. et al., Biochem . Pharmacol ., 69 (2), pp 221-32, 2004

[Reference 9] JimC. et al., J. Cell Sci . , 114 (Pt 12), pp2335-44, 2004

[Patent 10] Japanese Patent Laid-Open No. 4-9320

[Document 11] Japanese Patent Laid-Open No. 6-192062

[Patent Document 12] Japanese Patent Laid-Open No. 56-7710

[Patent 13] Japanese Patent Laid-Open No. 4-9315

[Document 14] A Study on the Whitening Effect of Sang-Hee Lee, Ph.D. Kyung Hee University Graduate School of East-West Medicine, 2001

[Study 15] A Study on the Whitening Effect of 加減 西 施玉容 散. Korean Journal of Ophthalmology & Otolaryngology & Dermatology. 15 (2), pp 104-117, 2002

16 Hosoi J. et al., Cancer Res ., 45, pp 1474-1478, 1985

Document 17 Mason HS, Paterson EW, Biochim . Biophys . Acta . , 111, pp134-146, 1965

Ultraviolet rays are a major cause of skin aging and wrinkle formation. UV light is divided into UVA, UVB, and UVC according to the wavelength. UVB (290-320 nm) penetrates the skin's epidermis, stimulates melanin synthesis, and UVA (320-400 nm) reaches the dermis. Induces the generation of free radicals and damages skin keratinocytes and dermal cells. External stress such as UV secretes TGF-beta, PGE2, etc. from the keratinocytes with growth hormones such as α-MSH, and these cytokines bind to receptors present in melanocytes to activate cellular functions. X et al., Mol . Cell Biol ., 27 (1), pp 283-96, 2007). Activated melanocytes synthesize melanin, a polymer of phenols, in the melanosome, which acts as part of a mechanism to protect the skin from external stress (Romero GC et al. J. Clin) . Invest. , 99 (4), pp 635-642, 1997). Melanin produced from melanocytes stimulated by α-MSH secreted by keratinocytes is transmitted to keratinocytes again through dendrite and the like to suppress the cellular injury caused by stress. Ahn So-Ryang, Seung-Jae Lee, Korean Journal of Beauty Research, 6 (1), pp239-254, 2000).

The synthesis of melanin is accomplished through the action of tyrosinase present in the melanosome of melanocytes. This tyrosinase acts as a tyrosine hydroxylase that oxidizes L-tyrosine to make L-DOPA, and a dopa oxidase that oxidizes dopaquinone to form dopaquinone. with an enzyme that acts synthetic melanin (山孝一良and hearing, VJ.メラニソ産生の制御因子, Fragnance Journal , 6, pp 24-28, 1990). Therefore, in the development of skin whitening agents that inhibit melanin production, �²� which inhibits the activity of tyrosinase is essential. TRP-2 (tyrosinase related protein-2) is an enzyme known as dopachrome tautomerase that produces DHICA (5,6-dihydroxy indole-2-carboxylic acid) using dopachrome. do. DHICA (5,6-dihydroxy indole-2-carboxylic acid) is oxidized by tyrosinase related protein-1 (TRP-1) to produce indole-2 -carboxylic acid (IQCA), which is indole-2 -carboxylic acid ) Is dark brown (Jackson IJ et al. EMBO J. , 11, 519, 1992).

Meanwhile, when melanocytes are activated and melanogenesis progresses, the activity of adenyl cyclase (adenylate cyclase) increases to increase the concentration of cAMP (cyclic adenosine monophosphate). PKA (protein kinase A) is a cAMP-dependent protein kinase that regulates the activity of various enzymes and is involved in cell proliferation. Increased PKA activity leads to increased phosphorylation and activity of tyrosinase and this process leads to increased melanin synthesis (Lee J. et al., Biochem . Pharmacol . , 74 (7) pp960-8, 2007). Therefore, when PKA expression is inhibited, melanin production is inhibited through prevention of tyrosinase activation. Protein kinase C (PKC) phosphorylates several enzymes to increase activity and stimulate cell proliferation. Increased PKC activity increases melanocyte function, leading to increased phosphorylation and activity of tyrosinase. Therefore, inhibition of PKC expression inhibits melanin production by inhibiting tyrosinase activation. Serine / threonine kinase (AKT) present in melanocytes, together with extracellular signal-regulated kinase (ERK), cyclic-AMP response element binding protein (CREB), and ribosomal S6 kinase (RSK-1) Transfer proteins are known to be factors that regulate gene expression (Huang SC et al., Biochem. Pharmacol . , 69 (2), pp221-32, 2004). Increased expression of ERK and AKT (serine / threonine kinase) inhibits the expression of MITF (microphthalmia transcription factor), which inhibits tyrosinase expression (JimC. Et al., J. Cell Sci . , 114 (Pt 12), pp 2233-44, 2004). Inhibition of the MITF (microphthalmia transcription factor) can inhibit the production of melanin through the inhibition of the expression of tyrosinase. Therefore, the development of pigment inhibitors by inhibiting the expression of MITF can be significant.

Ascorbic acid (Japanese Patent Laid-Open Publication No. 4-9320), hydroquinone (Japanese Patent Laid-Open Publication No. 6-192062), kojic acid (Japanese Patent Laid-Open Publication No. 56-7710), and arbutin (Japanese Patent Laid-Open Publication No. 4-9315) ) And some compounds have tyrosinase inhibitory activity and are used as raw materials for whitening cosmetics, but due to poor stability in the prescription system, they are degraded and colored, or off-flavor, efficacy at the biological level, unclear and safety The use is limited due to problems. And the inhibitory effect of tyrosinase has been demonstrated, but the effect is low in experiments similar to the actual biologic level. Therefore, the inhibitory effect of the melanocyte cell culture similar to the biological level is required. Hydroquinone is also regulated as a carcinogenic substance and is prohibited from use. Kojic acid and ascorbic acid are very unstable substances and browning occurs when a small amount of cosmetics are stored for several weeks at room temperature.

In order to solve the problems of existing raw materials with whitening effect by suppressing melanin production for a long time, various kinds of abnormal melanin production can be suppressed from high-efficiency plant extracts such as herbal medicines, vegetables, fruits, and flowers. Efforts have been made to find extracts that have an effect.

As a result of research on whitening with herbal medicines, the whitening effect of ephedra, ephedra and sabaeksan has been reported (Lee Sang-hee, research on the whitening effect of huang and huang), Graduate School of East-West Medical Science, Kyung Hee University, 2001, etc. The whitening effect of multiple prescriptions has also been reported (Song et al., Et al., A Study on the Whitening Effect of Ginseng sulphate, Journal of Ophthalmology and Otolaryngology and Dermatology, 15 (2), pp104-117, 2002).

Drainage is a perennial vine grass, with vine lengths growing up to 3-4 m. The rhizome is a hard wood, stretched to the side, and ends have various shaped tubers. The stems with tuber bark and the vertical strip of wood are called reddish red.

The lump of red sessau contains oxymethylanthraquinone derivatives, about 45% of horses, 8% of oil, and lecithin.Its effects include liver, kidney, and blood, and strengthen tendons and bones. It is known that it is used for tuberculosis, tuberculosis, tuberculosis, hemorrhoids, and postpartum disease, due to lack of blood and essence.

The present inventors to complete the present invention by confirming the melanin production inhibitory effect, tyrosinase activity inhibitory effect and tyrosinase, TRP-1, TRP-2, PKA, PKC, MITF gene expression inhibitory effect It became.

In order to achieve the above object, the present invention provides a skin whitening and skin anti-aging cosmetic composition containing a drop sewage extract as an active ingredient.

Extracts as defined herein include water, lower alcohols having 1 to 4 carbon atoms or mixed solvents thereof, hexane, propylene glycol, ethyl acetate, butylene glycol, ether or chloroform, preferably lower alcohols having 1 to 4 carbon atoms, more Preferably the extract is soluble in ethanol or chloroform.

Hereinafter, the method for obtaining the extract of the present invention will be described in detail.

The dripping sewage extract of the present invention is chopped dried dripping sewage, 3 to 50 times the weight (g), preferably 10 times the weight ratio of water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, hexane, propylene Glycol, ethyl acetate, butylene glycol, ether or chloroform, preferably lower alcohol or chloroform having 1 to 4 carbon atoms, more preferably ethanol or chloroform, 20 to 100 ° C., preferably 50 to 80 ° C. Extraction of sewage extract of the present invention can be obtained by extraction methods such as hot water extraction, reflux cooling extraction, ultrasonic extraction, and preferably reflux cooling extraction for minutes to 20 hours, preferably 1 to 5 hours.

The present invention provides a skin whitening and skin anti-aging cosmetic composition containing a drop of sewage extract obtained by the above manufacturing process as an active ingredient.

The drip extract is a skin whitening and skin anti-aging cosmetic composition, the extract can be used in 0.001 to 90% by weight, preferably 0.01 to 10% by weight based on the total weight of the composition.

In addition, the cosmetic composition comprising the drip extract of the present invention includes a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polysaccharides, sphingolipids and seaweed extract. The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, acyl acetate d1-alphatocopherol, nicotinic acid d1-alpha tocopherolvitamin E. DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenylethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle. The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be cosmetically blended, but may preferably include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate and argin purified from these seaweed extracts may be used. Potassium ginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta lysitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearate isostearic acid , Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, citric acid Triether, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate Dioctyl sebacate, stearic acid cholesterol, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, phosphate oleate , 12-stealoyl hydroxystearate isocetyl, 12-stealoyl hydroxystearate stearic acid, 12-s Allo one hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

As silicone fats and oils, polymethine silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methyl steoxysiloxane copolymer, and alkyl modified silicone Oil, amino modified silicone oil, etc. are mentioned.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

As animal or vegetable fats and oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil , Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum, Marker demia nut oil, Meadow home oil, Egg yolk oil, Ujima oil, Mink oil, Orange rape oil, Jojoba oil, Canderry wax, Carnabas wax And animal or plant fats and oils, such as liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As a water-soluble low molecular moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone- carboxylate titanium, glycerin, propylene glycol, 1, 3- butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned. Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters. As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose , Water soluble chitin, chitosan, dextrin and the like. Examples of the fat-soluble polymers include polyvinylpyrrolidoneecocene copolymers, polyminylpyrrolidone hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned. Examples of nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE glycerin fatty acid esters, and POE alkyls. Ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POEPOP (polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. Can be mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

Examples of cationic surfactants include alkyltriethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylmenzyl ammonium, behenyltrimethylammonium, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and composites thereof; Polyamide, Polyester, Polypropylene, Polystyrene, Polyurethane, Vinyl Resin, Urea Resin, Phenol Resin, Fluoro Resin, Silicon Resin, Acrylic Resin, Melamine Resin, Epoxy Resin, Polycarbonate Resin, Divinylbenzene Styrene Copolymer, Silk Organic pigments such as powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentel salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cyanic acid octyl, daparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-ρ- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

 Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, Is formulated at 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors and Carrier.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , Fatty acid esters of 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carriers such as water, ethanol or propylene glycol, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Drainage extract according to the present invention shows melanin production inhibitory effect, tyrosinase activity inhibitory effect and tyrosinase, TRP-1, TRP-2, PKA, PKC, MITF gene expression inhibitory effect, skin whitening and skin It can be usefully used as an anti-aging cosmetic composition.

1 is a diagram showing the effect on melanin release from B16F10 cells according to the concentration of drip extract,

2 is a diagram showing the effect on the activity of mushroom tyrosinase (mushroom tyrosinase) according to the concentration of the drip extract;

Figure 3 is a diagram showing the effect on melanin production from B16F10 cells according to the concentration of drip sewage,

Figure 4 is a view showing the effect on the tyrosinase expression according to the concentration of Drip Sewage,

5 is a diagram showing the effect on the expression of TRP-1 (tyrosinase related protein-1) according to the concentration of drip,

6 is a diagram showing the effect on the expression of tyrosinase related protein-2 (TRP-2) according to the concentration of drip,

7 is a diagram showing the effect on the expression of PKA (protein kinase A) according to the concentration of drip,

8 is a diagram showing the effect on the expression of PKC (protein kinase C) according to the concentration of drip,

Figure 9 is a diagram showing the effect on the expression of microphthalmia transcription factor (MITF) according to the concentration of dropping water.

Hereinafter, the present invention will be described in detail by the following. However, the following Examples, Reference Examples and Experimental Examples are merely illustrative of the present invention, but the content of the present invention is not limited thereto.

Example 1 Preparation of Soluble Ethanol Soluble Extract

100 g of dripping sewage (Wanju, Jeonbuk) was refluxed in ethanol (1 L) for 2 hours (70 ^o C), concentrated under reduced pressure and freeze-dried to obtain 13.8 g of ethanol extract (hereinafter referred to as “PM”). After dissolving in DMSO (dimethylsulfoxide, Sigma), sterilized by using a filter paper (Milipore) having a pore size of 0.22 um and refrigerated until use. DMEM (Dulbecco's Modified Eagle Medium, GiPMo BRL Co. USA) medium was used as a sample of the following experiment to fit the experimental concentration immediately before use in cultured cells.

Example 2 Preparation of Chloroform Fraction Extract of Drainage

PM 1L obtained in Example 1 was concentrated under reduced pressure, and then resuspended in 1 L of distilled water. 200 ml of chloroform was added to the resuspended mixture, and the mixture was shaken vigorously and extracted, and a chloroform layer was taken using a separatory funnel. This process was repeated three times, and the obtained chloroform layers were combined, concentrated under reduced pressure, and lyophilized to obtain 4.2 g of a sample (hereinafter referred to as "PM-C").

Reference Example 1. Cell Culture Method

Melanosite B16F10 cells (Seoul National University Cell Line Bank) were treated with DMEM medium (Dulbecco's Modified Eagle Medium, GiPMo) containing 10% FBS (Fetal bovine serum, Gibco) and penicillin / streptomycin (100 IU-50 ug / ml, Sigma). BRL Co. USA) was used and medium was changed once a day. FBS was dissolved at room temperature to inactivate the remaining complement components and then heat inactivated (heated for 30 minutes in a 56 ° C. water bath), and the medium was used after filtration with a 0.2 μm membrane filter (Gibco). In each cell, 100 μl per well was put into a 96-well plate (Surin Science, Korea) and 500 ul per well was put into a 24-well plate. To recover the cells, add 1 ml of trypsin-EDTA and react for 1 minute at 37 ° C to separate the adhered cells, add 4 ml of medium, wash them by centrifugation (1,000 rpm, 3 minutes), and then use them in the experiment. Frozen in liquid nitrogen. The cells were cultured in a CO ₂ incubator using 37 ° C., 5% CO ₂ and used in the following experimental examples.

Experimental Example 1. Measurement of melanin free inhibitory effect

Hosei J. et al., Cancer for the study of melanin free inhibitors Res . , 45, pp1474-1478, 1985) was performed as follows.

The B16F10 cells of Reference Example 1 were placed in a 6-well plate at a concentration of 2 × 10 ⁴ cells / ml, and incubated for 6 hours, followed by incubation for 1, 2, and 3 days by adding the PM obtained in Example 1 by concentration. Cells were then isolated by treatment with 0.05% trypsin-EDTA, and then diluted with PBS to measure the number of cells with a hematocytometer (Hematocytometer, Fuchs-Rosenthal, Germany). When exposed to external stress such as UV, it is released from skin keratinocytes, and control group treated with only α-MSH 10 μM, a type of growth hormone that binds to receptors present in melanocytes, and α-MSH 10 μM and cytotoxicity Melanin outflowed into the cell culture was measured by dividing the test group, which was treated with PM 300, 150, 75, and 37 ug / ml, which had no concentration, for 72 hours. From about 24 hours, melanin production could be observed under a microscope.

Experimental results, as shown in Figure 1 compared to the control group treated with α-MSH 10 uM melanin production of the test group treated with a concentration of 300, 150, 75, 37 ug / ml PM concentration was significantly inhibited significantly Could.

Experimental Example 2 Determination of the Effect on Mushroom Tyrosinase Activity

In order to determine the effect on tyrosinase activity, experiments were performed using Masson and Peterson et al. (Mason HS, Paterson EW, Biochim. Biophys. Acta. , 111, pp134-146, 1965) as follows.

0.1 M phosphate buffer (pH 6.8) 150 ul, 3 mM L-tyrosine aqueous solution 20 ul and the PM obtained in Example 1 were treated with 300, 150, 75, 37 ug / ml concentration of α-MSH 10 uM together One test group and a control group treated with only 10 uM of α-MSH were added 20 ul each, followed by addition of 10 Uul of 2500 U / ml mushroom tyrosinase (Sigma) to start the reaction. The reaction was incubated for 30 minutes at 37 ℃ was measured for absorbance at 405 nm at intervals of 10 minutes, to determine the tyrosinase activity using the following equation (1).

As a result, it was confirmed that tyrosinase activity was inhibited in a concentration-dependent manner as shown in FIG. 2 at PM 300, 150, and 75 ug / ml.

Tyrosinase Activity (%) = (B-B ') / (A-A') x 100

A: absorbance of the control reaction solution

A ': uptake of reaction solution in which buffer was added instead of tyrosinase in the control group

B: absorbance of the reaction solution to which the test solution was added

B '  : In B reaction solution Tyrosinase  Internal Buffer  Absorbance of the reaction solution

Experimental Example  3. Measurement of melanin production

To investigate the inhibitory effects of melanin biosynthesis, the method of Jose et al. (Hosoi J. et al., Cancer Res . , 45, pp1474-1478, 1985) was performed as follows.

In the cultured B16F10 cells of Reference Example 1, only the test group treated with the concentrations of 300, 150, 75, 37 ug / ml and α-MSH 10 uM and the α-MSH 10 uM were treated with the PM obtained in Example 1 above. One control was incubated for 2 days, then washed twice with PBS and centrifuged for 5 min at 1,500 x rpm. 300 ul of 1 N sodium hydroxide containing 10% DMSO was added and dissolved at 80 ° C. for 1 hour. To quantify melanin produced in cells, absorbance was measured at 475 nm and determined using synthetic melanin as a standard. After washing again with washing buffer three times and adding 100 μl of the enzyme conjugate (enzyme conjugate), and incubated for 45 minutes at 22 ^° C. After washing 3 times, 100 μl of substrate solution was added, incubated for 30 minutes at 22 ^° C., 50 μl of stop solution was added, and the absorbance value was determined at 450 nm.

As a result, as shown in FIG. 3, it was confirmed that melanin production was inhibited in a concentration-dependent manner in the test group treated with PM 300, 150, 75, 37 ug / ml, as compared to the control group.

Experimental Example  4. Impact on gene expression

In order to determine the effect on tyrosinase, TRP-1, TRP-2, PKA, PKC and MITF gene expression of the PM extract of Example 1 was performed as follows.

4-1. gun RNA  detach

1 ml trizol to a negative control group treated with only α-MSH 10 μm in B16F10 cells cultured in Reference Example 1 and a test group treated with a concentration of PM 200, 100, 50, 25 μg / ml and α-MSH 10 μm The solution (Invitrogen, USA) was processed to separate total RNA. 100 μl phenol and 100 μl chloroform: isoamyl alcohol (24: 1) were added to the isolated RNA, and the mixture was mixed well and centrifuged twice to separate the supernatant. RNA is precipitated using 0.5 ml isopropyl alcohol, washed with 70% ethanol and dried naturally. After RNA was dissolved in RNAase free water (Invitrogen, USA), RNase-free DNase (Invitrogen, USA) was added and stored at -70 ° C.

4 -2. cDNA manufacturing

Up to the experimental example of the negative control group isolated from 4-1 (the "C" La name card) and the test group each total RNA solution (containing 13 ug RNA) and mixed gently and then insert the dT 1 ㎕ Watch Next, 70 ^o Incubated for 5 minutes at C. Leave primers at room temperature for about 10 minutes to release the primers, then add cyscript buffer, 0.1 M DTT, dUTP nucleotides, dUTP seeded-labeled nucleotides, Cyscript reverse transcriptase, and then add Mix carefully. Thereafter, the cells were incubated at 42 ^° C. for 90 minutes and then left on ice. 2.5 M sodium hydroxide was added thereto, followed by incubation at 37 ^° C. for 15 minutes, and neutralized by adding 2 M HEPES buffer to prepare cDNA.

4-3. Reverse Transcription - Polymerase Chain Reaction  ( RT - PCR )

OligodT 12-18 (Promega, USA), reaction buffer (50 mM tris-hydrochloric acid, 75 mM potassium chloride, 3 mM magnesium chloride, 10 mM DTT, pH 8.3), 1 mM dNTP (Biotools, Spain) and 200 unit M CDNA was synthesized by reverse transcription by processing -MLV-RT (Moloney murine leukemia virus reverse transcriptase, Promega, USA). PCR was performed by adding cDNA and 1.25units of Taq polymerase (Biotools, Spain) to a mixture containing 10 × PCR buffer, 0.2 mM dNTPs, 2 pmole sense and antisense primers in a total volume of 15 μl. The control (hereinafter referred to as "N") represents the value of vehicle without the addition of the primer. PCR conditions were 94 ° C. 4 min, 30 cycles [94 ° C. (30 sec), 59 ° C. (30 sec), 72 ° C. (45 sec), 72 ° C. 10 min (Perkin Elmer, USA). 2% agarose gel was electrophoresed and analyzed using Gel-Pro analyzer 3,1 (Gel-Pro analyzer 3.1, Media Cybernetics, USA).

4-4. Real time RT - PCR

5 ug of RNA, 50 ng / μl of random hexamer, 3 μl of random hexamer and 1 μl of 10 mM dNTP were added to the test tube, and DEPC-H ₂ O was added to prepare 10 μl of RNA / primer mixture. Experimental samPMe was incubated at 65 ° C. for 5 minutes and then left on ice for at least 1 minute. The reaction mixture was prepared by mixing 2 µl of 10-fold RT buffer, 4 µl of 25 mM magnesium chloride, 2 µl of 0.1 M DTT, and 1 µl of RNAaseOUT (Promega, USA). The reaction mixture was added to the RNA / primer mixture, left to stand at room temperature for 2 minutes, then 1 μl (50 units) of SuperScript II RT (Promega, USA) was added and incubated at 25 ^° C. for 10 minutes. Incubated at 42 ^o C for 50 min, then inactivated by heating at 70 ^o C for 15 min and cooled on ice. 1 μl of RNase (Promega, USA) was added, incubated at 37 ^° C. for 20 minutes, and then stored at −20 ^° C. until use. Into each optical tube (Promega) add 12.5 μl of 2x SYBR Green Mix (promega), 0.2 μl cDNA, 1 μl 5 pmol / μl primer pair mixture, 11.3 μl H ₂ O , 50 ^° C. 2 minutes 1 cycle, 95 ^° C. 10 minutes 1 cycle, 95 ^° C. 15 seconds, 60 ^° C. 30 seconds, 72 ^° C. 30 seconds 40 cycles, 72 ^° C. 10 minutes 1 cycle. After completing the PCR, the tube was taken out, and PCR specificity was measured on a 3% agarose gel using 5 ul of the reaction solution. Real time PCR results were analyzed using SDS 7000 software. Statistical treatments were compared individually using Student's t-test and determined that there was a significant difference when the p-value was less than 0.05.

As a result, as shown in Figures 4 to 6, tyrosinase, tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2) increased expression in the negative control group, Example Gene expression increased at a concentration of 200, 100, 50, 25 ug / ml obtained in 1 was confirmed to be significantly suppressed in a concentration-dependent.

As shown in FIG. 7, PKA (protein kinase A) significantly inhibited the increased gene expression at PM 200 and 100 ug / ml concentration, but did not affect PM 50 and 25 ug / ml concentration. .

In addition, PKC-β (protein kinase C-β) and MITF (microphthalmia transcription factor) as shown in Figures 8 to 9, the expression was increased in the negative control group, PM 200, 100, 50, 25 ug / ml Gene expression increased in concentration was confirmed to be significantly suppressed in a concentration-dependent.

Therefore, it was confirmed that the PM obtained in Example 1 has an excellent effect on inhibiting tyrosinase, TRP-1, TRP-2, PKA, PKC and MITF gene expression.

Recombinant protein Primer sequence Tyrosinase Sense: SEQ ID NO: 1 5'-GGC CAG CTT TCA GGC AGA GGT-3 ' Antisense: SEQ ID NO: 2 5'-CTA AAA CGG GTA CTT CGT GGT-3 ' TRP-1 Sense: SEQ ID NO: 3 5'-GCT GCA GGA GCC TTC TTT CTC-3 ' Antisense: SEQ ID NO: 4 5'-TCT GGT CGT CAC GTC GCA GAA-3 ' TRP-2 Sense: SEQ ID NO: 5 5'-GGC CAG CTT TCA GGC AGA GGT-3 ' Antisense: SEQ ID NO: 6 5'-CCG TGG GTA ACC AGT GTT GGC-3 ' PKA Sense: SEQ ID NO: 7 5'-TCC CGT TCC TGG TCA AAC TT-3 ’ Antisense: SEQ ID NO: 8 5'-AAG AAG CGA CTG GTC GGA TAT-3 ' PKC-β Sense: SEQ ID NO: 9 5'-AGA ACC ACA AAT TCA CCG CC-3 ' Antisense: SEQ ID NO: 10 5'-GTA GCT GTC TCT CCA AGA GT-3 ' MITF Sense: SEQ ID NO: 11 5'-TAG ACA TGC CAG CCA AGT CC-3 ' Antisense: SEQ ID NO: 12 5'-ATT TTT CCC TCG AGT GTC GC-3 ' GAPDH Sense: SEQ ID NO: 13 5'-CAG CCT CGT CCC GTA GAC AAA-3 ' Antisense: SEQ ID NO: 14 5'-CGG CCA CGA CTC ATA CAG CAC-3 '

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation Example 1 Preparation of Hydrophilic Ointment

9.00% PM of Example 1

White Vaseline 36.00%

Stearyl Alcohol 31.00%

Trace amounts of ether (or methyl) ρ-oxybenzoate

Propylene Glycol 19.00%

Sodium Lauryl Sulfate 2.40%

Trace amount of propyl ρ-oxybenzoate

Total 100.00%

Formulation Example 2 Preparation of Soft Cosmetics (Skin)

2.00% PM of Example 1

Glycerin 5.00%

Triethanolamine 0.50%

Tocopheryl Acetate 1.00%

Liquid Paraffin 5.00%

Squalane 5.00%

Macadamia Nut Oil 15.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

Carboxyl Vinyl Polymer 0.20%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation Example 3 Preparation of Milk Croissant

5.00% PM of Example 1

1,3-butylene glycol 4.00%

Glycerin 3.00%

Oleyl Alcohol 0.05%

Polysorbate20 1.00%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation Example 4 Preparation of Nutritious Cream

2.00% PM of Example 1

Glycerin 5.00%

Triethanolamine 0.50%

Tocopheryl Acetate 1.00%

Udon paraffin 5.00%

Squalane 5.00%

Macadamia Nut Oil 15.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

Carboxyl Vinyl Polymer 0.20%

Preservative traces

Trace amount

Purified water level

Total 100.00%

Formulation Example 5 Preparation of Massage Cream

2.00% PM of Example 1

Vaseline 5.00%

Liquid Paraffin 30.00%

Beeswax 3.00%

Polysorbate60 1.00%

Solbitan Sesquioleate 1.00%

1,3-butylene glycol 5.00%

Glycerin 5.00%

Preservative traces

Trace amount

Purified water level

Total 100.00%

<110> HWANG, Gwi Seo YOO, Dong Youl <120> Cosmetic composition comprising the extract of Polygonum multiflorum showing skin whit <130> DIF / 2007-10-0011 / SJ <160> 14 <170> KopatentIn 1.71 <210> 1 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> sense primer for tyrosinase <400> 1 ggccagcttt caggcagagg t 21 <210> 2 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for tyrosinase <400> 2 ctaaaacggg tacttcgtgg t 21 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> sense primer for TRP-1 <400> 3 gctgcaggag ccttctttct c 21 <210> 4 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for TRP-1 <400> 4 tctggtcgtc acgtcgcaga a 21 <210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> sense primer for TRP-2 <400> 5 ggccagcttt caggcagagg t 21 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for TRP-2 <400> 6 ccgtgggtaa ccagtgttgg c 21 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> sense primer for PKA <400> 7 tcccgttcct ggtcaaactt 20 <210> 8 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for PKA <400> 8 aagaagcgac tggtcggata t 21 <210> 9 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> sense primer for PKC-beta <400> 9 agaaccacaa attcaccgcc 20 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for PKC-beta <400> 10 gtagctgtct ctccaagagt 20 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> sense primer for MITF <400> 11 tagacatgcc agccaagtcc 20 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for MITF <400> 12 atttttccct cgagtgtcgc 20 <210> 13 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> sense primer for GAPDH <400> 13 cagcctcgtc ccgtagacaa a 21 <210> 14 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> antisense primer for GAPDH <400> 14 cggccacgac tcatacagca c 21

Claims (4)

Skin whitening and skin anti-aging cosmetic composition containing drip sewage extract as an active ingredient. The cosmetic composition according to claim 1, wherein the extract is an extract available in water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, hexane, propylene glycol, ethyl acetate, butylene glycol, ether or chloroform. According to claim 1, wherein the extract is a cosmetic composition, characterized in that contained in 0.001 to 90% by weight relative to the total weight of the composition. The method of claim 1, wherein the composition is a formulation selected from the group consisting of a lotion, skin, lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, cleansing, face wash, soap, treatment, essence Cosmetic composition.