KR20110090365A - The greening base-soil containing cereal fermented - Google Patents

Abstract

PURPOSE: Vegetation foundation soil containing a grain fermentation product is provided to promote the sprouting of seeds by supplying necessary nutrients. CONSTITUTION: Vegetation foundation soil contains a grain fermentation product, peat, granite soil, sewage sludge, pulp sludge, water purification precipitates, rhytidome, and cocopeat. The grain fermentation product is obtained by mixing grains, yeast, and purified water, and fermenting the mixture after injecting lactobacillus for 24~48hours at 18~35deg C.

Description

Green vegetation soil containing grain fermentation {THE GREENING BASE-SOIL CONTAINING CEREAL FERMENTED}

The present invention relates to a vegetation soil for greening containing grain fermentation.

Recently, the pollution of the soil and the destruction of the environment are rapidly increasing due to the acceleration of mechanization, industrialization, and scientific farming, and the number of microorganisms having the self-cleaning ability of the microorganisms against the pollutants in the soil is rapidly decreasing, and the damage caused by environmental destruction It is increasing day by day.

This environmental pollution not only has a rapid and direct effect on human life through the air or water, but has been exposed for a long time through soil and groundwater.

In particular, soil pollution during environmental pollution is wide due to the improper disposal of harmful substances, the use of pesticides, sudden environmental accidents, unreasonable development, there is a problem that it takes a lot of time and money to clean the soil once contaminated.

Contaminants that can cause such soil pollution include oils such as diesel oil, diesel oil, heavy oil, lubricants, aromatic hydrocarbons, heavy metals, and various pesticides. As a result, beneficial bacteria in the soil die, and nutrients are lost, making plants unable to grow healthy.

Soil disease caused by soil pollution is a sign that the relationship between soil microorganisms and plants has deteriorated. In particular, the accumulation of salts from toxic chemicals or fertilizers destroys the habitat of microorganisms and simplifies their species.

When this happens, only certain types of microorganisms multiply abnormally, breaking the balance in the soil and attacking the roots of fragile crops, paralyzing the coexistence and coexistence between them. Most cropland and farmland are in this state.

On the other hand, microorganisms in soil that have a significant effect on the growth of plants include yeast, yeasts, actinomycetes, lamentous fungi, photosynthetic bacteria, and lactic acid bacteria. have.

In more detail, the role of each fungus in the soil is as follows.

Yeasts produce substances that are effective for crops by using secretions from crop roots in the soil, amino acids, sugars, and other soil organic matter emitted by photosynthetic bacteria. In addition, it creates the substrate needed to multiply other effective microorganisms (lactic acid bacteria, actinomycetes), thereby improving soil quality.

Actinomycetes produce antibacterial substances by receiving amino acids produced by photosynthetic bacteria. These antimicrobial substances can suppress pathogens or create a pleasant environment for harmful fungi and bacteria.

Filamentous fungi, which are used for effective microorganisms among the filamentous fungi (fungus) group, have a strong ability to produce alcohol, and therefore have the ability to prevent the occurrence of maggots and other harmful insects, and are effective in separating odors.

Photosynthetic bacteria help the crops by producing the beneficial bacteria and produce bioactive substances to help the crops.

In addition, lactic acid bacteria have a strong bacteriostatic power of lactic acid by producing a lactic acid based on the sugars received from the yeast, and serves to inhibit the reproduction of harmful microorganisms and the rapid decay of organic matter.

These lactic acid bacteria interact with the microorganisms in the soil to promote soil improvement, pest control, organic decomposition, nutrient and water absorption, and growth.

That is, in order to improve the soil quality, various conditions such as harmony of microorganisms and ecological balance, environment necessary for the growth of beneficial microorganisms, and activation of enzymes capable of degrading pollutants must be satisfied.

In addition, conventionally, due to the use of chemical adhesives or wastewater sludge sludge on the artificial soil to prevent the loss of soil on the slope, environmental pollution occurs or the soil is acidified, there is a problem that the germination and rooting of the plant is difficult.

Therefore, the present inventors promote the growth rate of microorganisms capable of degrading soil contaminants or organic substances, thereby promoting the decomposition of harmful substances, effectively purifying contaminated soil, and providing nutrients necessary for plant growth on slopes, thereby improving seed germination. The research continued on how to facilitate the greening.

As a result, the inventors found that the soil mixed with the fermented cereals accelerates the decay of organic substances in the soil and converts them into useful elements for plants, which promotes seed germination and increases the cohesion between the compositions. Knowing that it was not learned by rainstorms, the present invention was completed.

It is an object of the present invention to provide a vegetation base for greening in which plants can grow healthy by inhibiting the growth of harmful strains in soil using live lactic acid bacteria and promoting the growth of harmless strains.

Another object of the present invention is to remove the odor generated from sewage sludge or pulp sludge by using a grain fermentation containing lactic acid bacteria, and to promote the decomposition of such organic matter to provide a vegetation base for greening to supply the nutrients necessary for plants It is to.

Still another object of the present invention is to provide a vegetation soil which enables slope greening construction and supplies nutrients necessary for plant growth to promote seed germination.

In order to achieve the above object, the present invention is 10 to 15% by weight cereal fermentation, 10 to 15% by weight peat, 20 to 30% by weight Masato, 5 to 10% by weight sewage sludge, 5 to 10% by weight pulp sludge It provides a vegetation soil for greening, consisting of 10 to 20% by weight of purified water precipitate, 5 to 10% by weight of bark, 5 to 10% by weight of coco peat.

The grain fermented product is mixed with 35 to 55% by weight of grains and 0.1 to 0.3% by weight of yeast to make up to 100% by weight of purified water, and the mixture is inoculated with lactic acid bacteria and fermented at a temperature of 18 to 35 ° C for 24 to 48 hours. You can get it.

The lactic acid bacteria may be included in an amount of 0.001 to 1 part by weight based on the total weight of the mixture of cereals, yeast and purified water.

The lactic acid bacteria may be Lactobacillus ( Lactobacillus ) genus uniform, the Lactobacillus ( Lactobacillus) genus is Lactobacillus Broken Crescent Needle (L. Buchneri) or Lactobacillus para Kasei may be all one or two selected from (L. Paracasei).

The grain fermentation may include bacteriocin produced by the bacteria Lactobacillus .

The present invention has the effect of providing a vegetation soil for greening that can promote the greening of soil using grain fermentation.

In addition, sewage sludge, pulp sludge and purified water precipitates can increase the cohesion between the compositions so that the slope can be recorded so that the slope is not leaked even when the slope area is mounted.

And by supplying the nutrients necessary for plant growth, it can promote the germination of seeds to provide a competitive vegetation soil.

Figure 1 is a photograph showing the results of the efficacy test of cereal fermentation in Example 2 and Comparative Example 1.
2 is in Experimental Example 1, It is a picture showing the results of the efficacy test of the recording soil according to the present invention.

Simple modifications and variations of the present invention can be easily made by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

The vegetation soil for greening according to the present invention is made by mixing grain fermentation products, minerals and soil supplements. Peat and masato may be used as minerals, and sewage sludge, pulp sludge, purified water sediment, bark and coco peat may be used as subsidiary soil supplements.

More specifically, the vegetation soil for greening is 10 to 15% by weight of grain fermented product, 10 to 15% by weight peat, 20 to 30% by weight of Masato, 5 to 10% by weight of sewage sludge, 5 to 10% by weight of pulp sludge, It may be made by mixing 10 to 20% by weight of purified water precipitate, 5 to 10% by weight of bark, and 5 to 10% by weight of cocoite.

The method for producing the grain fermented product will be described in detail below.

35 to 55% by weight of grains and 0.1 to 0.3% by weight of yeast is mixed with purified water to make 100% by weight, and the mixture is inoculated with lactic acid bacteria and fermented at a temperature of 18 to 35 ° C for 24 to 48 hours to obtain a grain fermented product. Can be.

The lactic acid bacteria may be used without limitation as long as they belong to the genus Lactobacillus . More specifically, Lactobacillus probe immense Li (L.Buchneri), Lactobacillus para Kasei, but can be used alone or two kinds of bacteria selected from (L. Paracasei), but are not necessarily limited thereto.

The lactic acid bacteria may be included in an amount of 0.001 to 1 part by weight based on the total weight of the mixture of cereals, yeast and purified water.

When the lactic acid bacteria is less than 0.001% by weight, the fermentation efficiency of the lactic acid bacteria may be low, and when it exceeds 30% by weight it is preferable to limit within the above range to affect the growth of lactic acid bacteria.

The grain fermented product is determined according to the selected species, the species of lactic acid bacteria.

In other words, the grain, a yeast and, if the mixture of purified water Lactobacillus probe into the immense Li (L. Buchneri), Lactobacillus para Kasei (L.Paracasei) Cereal fermentation has Lactobacillus probe immense Li (L. Buchneri), L. Paracasei is present.

The grains include all the general grains known in the art. Specific examples include, but are not limited to, one or two or more selected from rye, wheat, oats, barley, rice, and brown rice.

When the blending ratio of the grains is less than 35% by weight, it may be economically inefficient due to the production of a small amount of grain fermentation, and when it exceeds 55% by weight, a problem that the fermentation takes too long may occur.

In addition, the yeast can use any known in the art. As a specific example, it is preferable to use saccharomyces cerevisiae , but it is not necessarily limited thereto.

The yeast is to mix 0.1 to 0.3% by weight, the fermentation efficiency of the yeast may be low when less than 0.1% by weight, it is preferable to limit within the above range to affect the growth of the yeast when more than 0.3% by weight .

In addition to yeast, the grain fermentation may be added with an enzyme to promote fermentation. In particular, the liquefied enzyme for food is excellent in the degradability of the substrate, the functionality of the fermentation product, PH conditions, etc., it is preferable to use it because it is cheap in terms of price.

And the fermentation conditions are preferably fermented for 24 to 48 hours at a temperature of 18 to 35 ℃. The temperature is a temperature at which the strain is good to live. Since the fermentation period does not change the strain amount even after 48 hours, it is preferable to ferment during the time.

The metabolite produced by the lactic acid bacteria can solubilize hardly degradable organic substances such as lignin or cellulose in the bark and coco peat in the soil. It also plays an important role in eliminating various harmful effects of undecomposed organic matter and fermenting and decomposing organic matter.

In particular, the lactic acid bacteria to promote the fermentation decomposition of sewage sludge, pulp sludge, water purification sediment containing a large amount of organic matter to be described later to supply the nutrients necessary for plant growth. In addition, by decomposing the toxic components of the soil (Lindane), aliphatic hydrocarbons, oil, benzene and the like to accelerate the decay of organic matter in the soil to provide improved soil quality.

In addition, lactic acid bacteria discharge the lactic acid bacteria to inhibit the growth and activity of harmful bacteria to create an environment in which other harmful microorganisms in the soil can grow well so that they can live together.

The use of such lactic acid bacteria can prevent powdery mildew or pests, which are plant diseases that form white powdery spots on the leaves and stems of plants, and improve soil, control pests, promote organic decomposition, promote nutrient and water absorption, and promote growth. We provide environmentally friendly methods that are harmless to animals and plants. In addition, the grain fermentation is economically advantageous because the manufacturing process is not complicated.

And these lactic acid bacteria produce bacteriocin. It is a natural antibiotic, and the bacteriocin's antimicrobial properties can inhibit the growth of harmful bacteria or fungi in the soil, which can promote the greening of the soil along with lactic acid bacteria. It is an antimicrobial protein composed of a protein produced by gram-positive bacteria and gram-negative bacteria or a complex of protein and carbohydrates.

In other words, bacteriocin forms colonies around the roots of the seeds, rejects bad bacteria in the soil and inhales only good ingredients, allowing nutrients to inhale into the roots, preventing acidification of the soil, and promoting seed development. Will be able to perform

Thus, the vegetation soil for greening according to the present invention contributes to the growth of plants or germination of seeds to achieve early greening of the soil.

On the other hand, vegetation soil for greening according to the present invention 10 to 15% by weight of peat, 20 to 30% by weight of Masato, 5 to 10% by weight of sewage sludge, 5 to 10% by weight of pulp sludge, purified sediment 10 It can be obtained by mixing 20 to 20% by weight, 5 to 10% by weight of bark, and 5 to 10% by weight of cocoite.

Hereinafter, the configuration and role of other compositions mixed in addition to the grain fermentation will be briefly described.

Peat is broadly included in one type of coal, but is generally distinguished from coal. Unlike wood, which was buried underground like coal, which was fished for a long time with acupressure and geothermal action, lignin cellulose, the main component of the plant, was mainly degraded at the surface. It is rich in nutrients, conservative and breathable, which is good for plant growth, and can be used for landscaping fertilizers, farms, fertilizer factories, and golf courses.

Peat in the present invention may be made by mixing 10 to 15% by weight.

In the present invention, when the peat is more than 15% by weight, it is preferable to add within the above range because excessively high preservative power and high moisturizing power, and less than 10% by weight, low preservative power and low moisturizing power.

Masato is a soil made by weathering granite, and is highly breathable, which helps plants grow by increasing contact with air. It is excellent in drainage and breathability, and facilitates the contact between plants and air to promote plant growth, and is used for pavement of field crops, landscaping plants, bonsai, flower pots, school grounds, tennis courts, walkways, etc.

Masato in the present invention may be made by mixing 20 to 30% by weight.

In the present invention, when Masato is more than 30% by weight, there is a disadvantage in that water falls out too quickly, and if less than 25% by weight, the air permeability may deteriorate.

Sewage sludge is sludge from sewage and wastewater, which is characterized by high organic content. And, pulp sludge covers like quilts and plays a role in improving seed adhesion and retention.

On the other hand, the purified water precipitate is a suspension of suspended solids in the precipitation and backwashing process of the water treatment process. These water-precipitated sediments have high organic content and good water retention, so they maintain proper moisturizing power for germination of mixed seeds, supplying the rich minerals and organic nutrients contained in the water-precipitated sediments, and the organic and inorganic substances necessary for plant production. It promotes development so that the growth of plants can be done normally during the dry season.

In the present invention, it may be made by mixing 5 to 10% by weight of sewage sludge, 5 to 10% by weight of pulp sludge, and 10 to 20% by weight of purified water precipitate.

However, if 5% by weight of sewage sludge, 5% by weight of pulp sludge, and 10% by weight of water precipitates are less than each lower limit, the content of organic matter is too small to supply useful components to plants, and 10% by weight of sewage sludge and 10% by weight of pulp sludge. It is preferable to add in the above range because% and 20 wt% of purified water precipitate may exceed the respective upper limit ranges, which may adversely affect the growth of plants.

The bark is a bark, which is highly breathable to help the plant grow. In the present invention it can be made by mixing 5 to 10% by weight of the bark.

In the present invention, if the bark is more than 10% by weight, there is a disadvantage that the air permeability is too good, if less than 5% by weight, there is a problem that the air permeability worsens, it is preferable to add within the above range.

Coconut is made from the shells left after eating coconuts. It is a non-toxic and odorless material that is resistant to microbial penetration and stable in oxidizing conditions and contains considerable organic nutrients and trace elements.

In addition, it has the property of not decomposing for a long time, the water holding power is very high and the breathability is good.

In the present invention may be made up to 5 to 10% by weight of cocoite.

In the present invention, when the coconut is more than 10% by weight, there is a disadvantage in preventing the decomposition of microorganisms, and when it is less than 5% by weight, it is preferable to add the coconut within the above range because it does not have moisture control ability.

This coco nut has no environmental pollution, moisture absorption capacity is 3 times that of sawdust and fermentation period is 1/2 of sawdust, which is the best as an existing moisture control agent. This has the advantage of being stable.

Therefore, the vegetation soil for greening according to the present invention can be added to the blender to the blender to be sprayed onto the soil containing harmful substances or organic matter.

As described above, the vegetation soil for greening of the present invention inhibits the growth of harmful bacteria by the lactic acid bacteria and the metabolites of the lactic acid bacteria in grain fermentation products, promotes the growth of beneficial bacteria, and diversifies the poor microorganisms in the soil. Providing the main nutrients needed for plant growth can promote greening of the soil.

Hereinafter, the structure of the present invention will be described in more detail with reference to examples, but the scope of the present invention is not limited to the following examples.

Example  One

Wheat 40% by weight, (saccharomyces yeast Saccharomyces on Mrs Serenity Vichy cerevisiae ) (KCTC 11237BP) 0.3% by weight, the rest is purified water to make 100% by weight, the mixture is L. Buchneri ( KCCM 40982) And Lactobacillus paracasei ( L. Paracasei , KCCM 32828) strains were mixed at a ratio of 1: 1 by adding 0.5 parts by weight to the total weight of the mixture of cereals, yeast and purified water, and then inoculated for 48 hours at 25 ° C. Fermented.

When the fermentation was completed, the fermentation product was filtered through a 1 micron pressure filter to obtain a grain fermentation product. As a result of measuring the strain number of the fermentation product using a hemocytometer of Thoma, Lactobacillus bacteria were present at 60 X 10 ⁴ CFU / ml per ml of fermentation product.

Example  2

15% by weight of the grain fermentation of Example 1, 15% by weight of peat (Eco Industry), 20% by weight of Masato (Danong), 10% by weight of sewage sludge (Seongnam sewage treatment plant), 10% by weight of pulp sludge (Onyang Pulp Co., Ltd.), 10% by weight of purified water sediment (Seongnam Water Treatment Plant), 10% by weight of bark (Punglim Blue Flower Garden), 10% by weight of coco peat (Coco Green, Sri Lanka) 5 g of lettuce seeds were planted on the vegetation soil.

Comparative example  One

Peat (Eco Industries) 25% by weight, Masato (Danong) 25% by weight, Sewage sludge (Seongnam sewage treatment plant) 10% by weight, Pulp sludge (Onyang pulp) 10% by weight, Purified sediment ( 5 g of lettuce seeds were planted on the vegetation base for greening, which was made by mixing 10% by weight of Seongnam Water Treatment Plant), 10% by weight of Bark (Punglim Blue Flower Garden), and 10% by weight of Cocopit (Coco Green, Sri Lanka).

The lettuce seeds of Example 2 and Comparative Example 1 were grown for 20 days in a greenhouse at room temperature 20 ℃.

Figure 1 (a) is a comparative example (b) is a picture showing the appearance of lettuce seeds grown according to the embodiment. It can be seen from Fig. 1 that the lettuce seeds grown in artificial soil added with grain fermentation grew much faster.

In other words, the grain fermented product can be found to promote the decomposition of organic matter and harmful components in peat, masato, sewage sludge, pulp sludge, purified water sediment, bark, coco peat to provide an effective ingredient to plants.

Experimental Example  One

Experimental group (A) made of 70% by weight of the soil of the general soil mixed with the rest of Example 2 to 100% by weight and the control group (B) made of 100% by weight of the soil of Comparative Example 1 mixed with 70% by weight of the general soil After each bottle was sealed in a moist seal, the fermentation was stored for 3 days at a temperature of 30 ℃ and the smell and color change were confirmed.

Table 1 below shows the results of 10 olfactory tests on the soil. When comparing the experimental results of the experimental group (A) and the control group (B), it can be seen that the intensity of the smell of the experimental group (A) was weak.

Through the above results, it was found that the soil quality after a certain time in the case of the soil containing the grain fermented product according to the present invention. In other words, it can be seen that grain fermentation has the ability to remove odors and clean the environment.

In addition, it can be seen that the control group (B) has a dark color of black through FIG. 2, and the experimental group (A) includes a different color of color in the dark color of the black color. This showed that the decomposition of organic matter in the soil was promoted.

Olfactory test results Experimental group (A) Control group (B) One ++ +++ 2 + ++++ 3 + +++ 4 ++ ++++ 5 + ++++ 6 ++ ++ 7 + +++ 8 + ++ 9 +++ ++++ 10 ++ +++

※ + indicates the degree of smell.

Example  3

A soil-based vegetation soil 0.15 m 3 containing green cereal fermentate prepared in Example 2 and 250 g of plant seeds were mixed into a mixer, and the air compressor (discharge capacity 25) M 3 / H) was sprayed to a thickness of 1 mm over about 5 minutes and then 10 liters of water were sprayed.

As described above, the initial germination started after 3 to 6 days after the spraying, and after 7 to 9 days, the germination of all seeds was completed, and after 2 weeks, most of the fill was obtained.

In this way, the sewage sludge, pulp sludge, and the purified water precipitate increased the cohesion between the compositions, so that the slope was recorded so that the slope area was not leaked by the rain. In addition, seed germination was promoted by providing nutrients necessary for plant growth.

In other words, the base soil according to the present invention, "lactic acid bacteria and bacteriocin" which can invigorate the soil in the dead state can diversify the poor microorganisms in the soil. In addition, the symbiotic relationship between these microorganisms and plants is restored to stabilize the soil interior, thereby prematurely greening the soil.

Claims (6)

10 to 15% by weight of grain fermentation, 10 to 15% by weight peat, 20 to 30% by weight of masato, 5 to 10% by weight of sewage sludge, 5 to 10% by weight of pulp sludge, 10 to 20% by weight of water precipitate, 5 to 5% of bark Vegetation base for greening, characterized in that consisting of 10% by weight, cocoite 5 to 10% by weight. According to claim 1, wherein the grain fermented product is mixed with 35 to 55% by weight of grains and 0.1 to 0.3% by weight of yeast to make up to 100% by weight of the purified water, the mixture is inoculated with lactic acid bacteria at a temperature of 18 to 35 ℃ Vegetation soil for greening, characterized in that obtained by fermentation for 24 to 48 hours. According to claim 2, wherein the lactic acid bacteria Lactobacillus ( Lactobacillus ) is a vegetation soil for greening, characterized in that the genus. The vegetation soil for greening according to claim 2, wherein the lactic acid bacteria is contained in an amount of 0.001 to 1 parts by weight based on the total weight of the mixture of the cereals, yeast and purified water. According to claim 3, The Lactobacillus ( Lactobacillus) genus bacteria Lactobacillus L.Buchneri or A vegetation base for greenery, characterized in that one or two species selected from L. Paracasei . The vegetation soil for greening according to claim 1, wherein the grain fermented product includes bacteriocin produced by the Lactobacillus genus.

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