KR20110000082A - Cosmetic composition for controlling anti-acne - Google Patents

Abstract

PURPOSE: A cosmetic composition containing herbal material is provided to effectively suppress propionibacterium acne and to ensure skin moisturizing. CONSTITUTION: A cosmetic composition contains 0-.01-10 weight% of mixture extract containing 1-30 weight% of Sophorae Radix, 1-25 weight% of Lonicerae Flos, 1-15 weight% of Houttuynia Cordata, 1-20 weight% of Liliaceae, and 1-20 weight% of Portulaca oleracea. A method for preparing the mixture extract comprises: a step of pulverizing Sophorae Radix, Lonicerae Flos, Houttuynia Cordata, Liliaceae and Portulaca oleracea to obtain dried powder; a step of extracting the dried powder with a solvent including water, butylenes glycol and ethanol at 25-50°C for 10-48 hours and a step of filtering. The cosmetic composition is used in the form of a nutrition cream, massage cream, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, suspension, lotion or cosmetic liquid.

Description

Cosmetic composition for improving acne {Cosmetic composition for controlling anti-acne}

The present invention relates to a cosmetic composition containing an extract mixture extracted from a mixture comprising red ginseng, gold and silver, three hundred seconds, eungchocho, rosacea, machiseum, showing the efficacy of inhibiting acne bacteria, improving skin diseases and skin moisturizing power.

Acne is called acne vulgaris and is one of the growing skin features. In general, the cause of acne is not clear, but it is different from person to person and several factors are known to interact with each other. The main cause of this acne is first, excess sebum produced by the active sebum production of testosterone, an androgen hormone (androgenic hormone), and second, excess sebum produced by the closure of the pores by hyperkeratinized hair follicles in the hair follicles It is stagnant and promotes the formation of surface cells, which leads to an increase in acne bacteria. Third, an acne bacterium, an acne bacterium, secretes an enzyme called lipase, which causes excess sebum to be produced by hypertrophy of sebaceous glands and hyperkeratosis of hair follicle pores. One triglyceride is broken down to produce free fatty acids. The free fatty acids thus produced act on the skin epithelium to produce various enzymes and destroy the hair follicle walls, causing inflammation of connective tissue around the hair follicles, papules, pustules, cysts, and nodule. This is worse and causes scars in the worst case.

As a treatment for acne, surgical treatments such as scrim removal and the use of cosmetics using retinoic acid, benzoyl peroxide, tetracycline-based antibacterial agents and antibiotics are mainly used. Surgical treatment, however, has a problem that skin care should be continued after the procedure, and antimicrobial and antibiotic methods are known to cause side effects such as skin irritation and phototoxicity. Although researches for the development of cosmetics with less side effects on the skin are being actively conducted, the conventional results are known for cosmetics that complexly eliminate the factors of skin diseases caused by acne bacteria based on the results of analyzing oriental medicine ingredients in terms of oriental medicine. There is no bar.

It is an object of the present invention to provide a cosmetic composition having the effect of moisturizing, antibacterial, anti-inflammatory, soothing and so on of the skin by containing the extract mixture extracted from red ginseng, gold and silver, three hundred seconds, eoseongcho, rosacea and machi Prefecture will be.

In order to achieve the above object, the present invention relates to a cosmetic composition comprising an extract mixture extracted from a mixture comprising red ginseng, gold and silver, three hundred seconds, aseongcho, presbyopia and machi.

More specifically, the present invention comprises 1 to 30% by weight of red ginseng, 1 to 25% by weight of gold and silver coins, 1 to 15% by weight of three hundred seconds, 1 to 20% by weight of effervescent vinegar, 1 to 20% by weight of rosin, and 1 to 20% by weight of Machi. It relates to a cosmetic composition, characterized in that the extract mixture extracted from the mixture contains 0.01 to 10% by weight based on the total composition.

Ginseng is a perennial herb, and its root contains alkaloids d-matrin, d-oxymatrine, d-sophoranol, 1-methyl cyticin, 1-baptoline and 1-sopocarpine, and flavonoids xanthohumol , Isozantohumol, and camphorol. The contained component has an antimicrobial effect, which may have a synergistic effect on the antimicrobial activity against acne bacteria when mixed with sterling silver, rosacea and machi, which are the constituents of the present invention. Is preferably 10 to 20% by weight based on the extraction mixture. When mixed in the above components and the proportion according to the invention may have an antimicrobial effect against the effective acne bacteria.

Gold and silver are known to detoxify, diuretic, convulsive and relieve pain. In the present invention, by using a combination of presbyopia, gosam, machihyun in the above ratio rather than using gold and silver alone, it can have excellent antibacterial, free radical scavenging effect, anti-inflammatory effect on the skin, more preferably in the extract mixture It is good to include 10 to 17% by weight.

Three hundred seconds is known to be effective in eliminating constipation, but in the present invention when mixed with red ginseng, gold and silver, rosacea, Mihyeon, Eoseongcho may have a synergistic effect on the skin regeneration effect and moisturizing effect, more preferred The content ratio is preferably contained 10 to 13% by weight based on the extraction mixture, it may have an effective skin regeneration effect in the content ratio.

Echo vinegar is known to have anti-cancer, blood circulation promoting effect, in the present invention may have a more effective antimicrobial effect and acne skin regeneration and sedative effect when mixed with the composition and extracted, the echo vinegar more preferably extract mixture Containing 10 to 17% by weight of the present invention can optimally exert the antimicrobial and skin regeneration effects when each component is mixed.

Presbytery is effective for moisturizing skin and has a more effective skin moisturizing effect when included in the above range. When the content of presbyopia is more than 20% by weight, it is not suitable as a cosmetic because of its high viscosity, and less than 1% by weight of the moisturizing effect is insignificant. Machi Prefecture has an antibacterial and antifungal effect is effective in calming acne skin when included in the cosmetic composition according to the present invention.

It is preferable that the extract mixture contains 0.1 to 10% by weight with respect to the total composition, the greater the content of more than 10% by weight may reduce the moisturizing effect, the skin improvement effect may be low.

 In the present invention, the extraction mixture is characterized in that it is obtained by a method comprising the following steps.

 a) crushing and drying the red ginseng, gold and silver, three hundred seconds, eoseongcho, rosacea, machi string to prepare a dry powder of 0.1 to 1% by weight of moisture; And

b) extracting the dried powder by filtration and immersion at 25 to 50 ° C. for 10 to 48 hours with an extraction solvent containing a solution of water, butylene glycol and ethanol;

The extracting step in step b) is not particularly limited, and may be deposited for 110 to 48 hours, and more preferably 20 to 25 hours. The red ginseng, gold and silver, three hundred seconds, eoseongcho, roche, machihyeon can be prepared by extracting and mixing the dry powder, respectively, the dry powder mixture can be extracted, but not limited thereto.

In the present invention, more preferably, in step b), the extraction solvent may include all of water, butylene glycol, and ethanol, and extract the dry powder into an extract having a sufficient effect of calming acne skin, wherein the extraction solvent is dried. The water is preferably 400 to 800 parts by weight, 50 to 300 parts by weight of butylene glycol, 50 to 300 parts by weight of ethanol per 100 parts by weight.

The present invention is the extract mixture step b), after the step of drying to obtain an extract mixture dry powder having a water content of 0.1 ~ 1.0% by weight; And

Butylene glycol is added to and mixed with the extract mixture dry powder to obtain an extract mixture, wherein the butylene glycol is added in an amount of 50 to 300 parts by weight based on 100 parts by weight of the dry powder.

The cosmetic composition is used for the skin, such as softening water (nutrition fluid), nutrition cream, massage cream, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, suspension (suspension), lotion, or It may be prepared in the form of a conventional cosmetic such as cosmetic liquid, and may include various conventional carriers and additives suitable for each of these formulations and well known in the art. In addition, the composition may be made of a skin contact material in contact with the skin, such as cosmetics, detergents, and fibers.

The cosmetic composition according to the present invention may include an extract mixture extracted from red ginseng, sterling silver, triticale, eosungcho, rosacea and machi, and may have an effective suppression of acne bacteria and a skin moisturizing effect due to the synergistic action of the respective components.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the present invention is not limited to the following examples.

Production Example 1 Obtaining an Extract Mixture Extracted from Red Ginseng, Gold Silver Cod, 300 sec.

200 g of red ginseng, 170 g of gold and silver coins, 130 g of 130 seconds, 170 g of effervescent vinegar, 160 g of presbyopia, and 170 g of maize were ground and dried at room temperature to prepare a dry powder having a water content of 0.5% by weight.

1.0 kg of the dried powder was added to 1.0 kg of water 5.0 kg butylene glycol (DAICEL CHEMICAL INDUSTRIES.LTD.Lot. 13BGC-GI-004) and 1.0 kg of ethanol were mixed and mixed with 7.0 kg of the mixed solvent, and at 25 ° C. for 24 hours. After immersion it was extracted by filtration and concentration. The extracted extract was dried to obtain an extract mixture dried powder having a water content of 0.5% by weight, and 2 kg of butylene glycol was added and mixed to 1 kg of the extract mixture dried powder to obtain a liquid extract mixture.

Formulation Example 1 and Comparative Example 1 Preparation of Cosmetic Water

A lotion was prepared according to a conventional method according to the ratio of the lotion formulation of Table 1 containing the extract mixture obtained in Preparation Example 1.

Formulation Example 2 and Comparative Example 2 Nutritional Lotion

The nutrition lotion was prepared according to a conventional method in the proportion of the nutrition lotion formulation of Table 2 containing the extract mixture obtained in Preparation Example 1.

Formulation Example 3 and Comparative Example 3 Nutritional Cream

The nourishing cream was prepared according to a conventional method according to the nutrient cream formulation ratio of Table 3 containing the extract mixture obtained in Preparation Example 1.

Test Example 1 Antibacterial Effect Test

1) paper-disc assay

Propionibacterium acnes subcultured from KCTC were mixed with sterilized 2.80 g of Brucella broth and 100 mL of purified water, and the bacteria were diluted 10-fold. Create a microbial solution, and add 100 microliters to a solid medium made of sterile 2.15 g of Brucella agar, 2.60 g of Brain heart infusion agar and 100 mL of purified water Inoculation.

670 ㎍ of the extract mixture of Preparation Example 1, 1.33 mg of Extract Mixture of Preparation Example 1, 6.67 mg of Extract Mixture of Preparation Example 1, 0.1 g of Formulation Example 1 lotion, and 0.1 g of the lotion of Comparative Example 1, respectively, in 1 ml of methanol. Twenty microliters were dropped in 8-mm diameter paper-disc, and completely dried. After attaching the completely dried paper plate on the solid medium inoculated with the bacteria solution, and incubated for 1 to 2 days under anaerobic condition mixed with hydrogen, carbon dioxide and nitrogen at 35 ℃, inhibit the degree of inhibition of acne bacteria (Halo test, Zone of Inhibition test, AATCC 147), but the diameter of the inhibition zone (inhibition zone) was measured. The results are shown in Table 4 below.

As shown in the results of Table 4, Formulation Example 1 according to the present invention increased the antimicrobial area compared to Comparative Example 1. The cosmetic composition comprising the extract mixture according to the present invention was found to have excellent antimicrobial activity. Although the cosmetic composition comprising the extract mixture according to the present invention showed a small triclosan and salicylic acid inhibitory activity, the triclosan salicylic acid is a pure substance, and the extract mixture according to the present invention is a mixture, and in view of the above, It can be seen that the antimicrobial activity of the extract mixture according to.

2) MIC assay

Acne bacteria that were subcultured from KCTC in a medium solution consisting of sterile Brucella broth 2.15g, Brain heart infusion broth 2.60g and purified water 100ml Propionibacterium acnes) was inoculated with 100 µl of the bacterial solution diluted to 10 ^-2 to make a bacterial solution. The inoculated medium solution was inoculated with 100 μl of the bacterial solution inoculated in 30 g of Preparation Example 1, Tetracycline, Formulation Example 1, and Comparative Example 1, respectively. 1 g of this was taken and then serially diluted using sterile distilled water (systematic dilution), and 200 µl each was put into a 96-well plate.

The plate was inoculated with a certain amount of bacteria solution at 1 × 10 ⁴ to 1 × 10 ⁶ cfu / mL, followed by incubation for 2 days under anaerobic conditions in which hydrogen, carbon dioxide and nitrogen were mixed at 35 ° C. to observe the degree of inhibition of acne bacteria. The minimum inhibitory concentration (MIC) of acne bacteria without growth was determined. The minimum inhibitory concentration refers to the minimum concentration of the drug that completely inhibits the growth of a given microorganism. Tetracycline was used as a comparative group. The results are shown in Table 5 below. As shown in Table 5, Preparation Example 1 according to the present invention is slightly weaker than tetracycline in the minimum inhibitory concentration for acne bacteria, but since the tetracycline is a pure substance and the Preparation Example 1 is a mixed extract, in view of the above, Preparation Example 1 according to the can be seen that the excellent antimicrobial activity.

Test Example 2 Anti-inflammatory Effect Experiment

Preparation Example 1 and Comparative Example 1 were dissolved in propylene glycol at a concentration of 1 g / ml, respectively. To each sample solution, 0.1 ml of 12.5 mM calcium chloride (CaCl ₂ ) was added to 0.05 ml of hyaluronidage enzyme solution (Sigma Chemical Co.) and reacted at 37 ° C. for 20 minutes, followed by 2.4 mg / ml hyaluronic acid. 250 μl of an acid (Hyaluronic acid) was added, and the enzyme reaction was performed at 37 ° C. for 1 hour. After centrifugation at 3000 rpm for 5 minutes, the change in absorbance was measured at 585 nm. At this time, the degree of inhibition of enzyme activity of pure methanol was measured together with the inhibition rate of the extract sample as a relative inhibition rate, and the anti-inflammatory effect was evaluated by the relative inhibition rate value. Formula 1 of the activity inhibitory effect (%) for observing the activity inhibition degree is as follows.

<Calculation Formula 1>

Hyaluronidase activity inhibitory effect (%)

= 100- (reaction absorbance of each sample / reaction absorbance of the control group × 100)

The results are shown in Table 6 below. According to Table 6, Preparation Example 1 according to the present invention is somewhat weaker compared to Allantoin (Allantion), but allantoin is a pure substance, the present invention is a mixed extract, in consideration of this point, according to the present invention Example 1 was found to be excellent antibacterial activity.

Test Example 3 Skin Safety Experiment

In order to determine the degree of skin irritation for the cosmetic composition according to the present invention, the following experiment was performed. Fifteen subjects were subjected to a patch test of the upper arm using a skin patch test kit (Haye's Teat Chamber). In this experiment, patients who had psoriasis, eczema acne, and other skin allergies, or pregnant women, breastfeeding mothers or contraceptives, and antihistamines were excluded. After wiping the test site with ethanol and drying it, 15 μg of the cosmetic composition prepared in Formulation Examples 1 to 3 was added dropwise to a chamber, and then fixed on the upper arm of the test site.

The information time was applied for 24 hours, and after removing the information, the test sites were marked with a marking pen (marking pen), and the test sites were observed after 24 hours, respectively, and evaluated according to the criteria of the following International Contact Dermatitis Research Association. Criteria for international contact dermatitis study are shown in Table 7, and the results evaluated according to the criteria are shown in Table 8. As shown in the results of Table 8, the formulations prepared according to the present invention showed that the average skin irritation degree was all zero and the cosmetic composition was less irritating to the skin.

Test Example 4 Measurement of Physical Property Stability

In order to evaluate the stability of the cosmetic compositions of Preparation Example 1 and Formulation Examples 1 to 3 can be used with room temperature, at room temperature, 40 ℃, daylight was generally measured for discoloration measurement used in the physical properties of the cosmetic components The results are shown in Table 9 below. From the results of Table 9, the cosmetic composition according to the present invention is very stable at room temperature and 40 ° C., but negligible discoloration is observed in about three weeks in daylight, but no further discoloration progresses, and thus thermal stability and light stability Eggplant was found to be a very stable material.

Table 1

[Table 2]

[Table 3]

Table 4

[Table 5]

Table 6

Table 7

Table 8

Table 9

Claims (5)

An extract mixture extracted from a mixture containing 1-30% by weight of red ginseng, 1-25% by weight of silver and silver, 1-15% by weight of 300 seconds, 1-20% by weight of eosungcho, 1-20% by weight of rosin, and 1-20% by weight of machi A cosmetic composition comprising 0.01 to 10% by weight based on the total composition. The method of claim 1, The extract mixture may include a) preparing dried powder having a content of 0.1 to 1% by weight by grinding dry ginseng, gold and silver, three hundred s, effervescent vinegar, rot, and machi. And b) extracting the dried powder by filtration and immersion at 25 to 50 ° C. for 10 to 48 hours with an extraction solvent containing a solution of water, butylene glycol and ethanol; Cosmetic composition, characterized in that obtained by including. 3. The method of claim 2, The extracting solvent in step b) is 400 to 800 parts by weight, 50 to 300 parts by weight of butylene glycol, 50 to 300 parts by weight of ethanol with respect to 100 parts by weight of dry powder cosmetic composition. The method of claim 3, wherein After step b), drying to obtain an extract mixture dry powder having a water content of 0.1 ~ 1.0% by weight; And Adding butylene glycol to the extract mixture dry powder and mixing to obtain an extract mixture, wherein the butylene glycol is added in an amount of 50 to 300 parts by weight based on 100 parts by weight of the dry powder; Cosmetic composition, characterized in that obtained further comprising. The method of claim 3, wherein The cosmetic composition is characterized in that it has a formulation selected from softening water (nutrition emulsion), nutrition cream, massage cream, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, suspension, lotion, or cosmetic liquid. Cosmetic composition.