KR20100128669A - A method of preparing a salt having whitening activity - Google Patents

Abstract

The present invention relates to a salt having a whitening activity and a preparation method thereof. In particular, the functional salt produced by the manufacturing method of the present invention is not only excellent in whitening effect, but also made of natural substances as an active ingredient does not cause problems related to resistance and safety, it is widely used in various uses including whitening cosmetics Can be.

Salt, whitening

Description

Process for producing salts with whitening activity {A METHOD OF PREPARING A SALT HAVING WHITENING ACTIVITY}

The present invention relates to a salt having a whitening activity and a preparation method thereof.

The main component of salt is sodium chloride (NaCl), which is physiologically indispensable for animals. Specifically, sodium chloride is contained in the body, in particular, body fluids to adjust the osmotic pressure of the body fluids, sodium as a component of sodium chloride is combined with phosphoric acid to maintain the acid group parallel to the body fluid as a buffer material, such as bile juice, interest solution or serous It forms an alkaline digestive fluid, acts as a neurostimulant, and chlorine, another component of sodium chloride, constitutes gastric juice.

Salt used as a seasoning to taste the food in daily life is a component that is closely related to the physiological activity and life support of the animal as described above, so if the salt intake is short-term, the secretion of digestive fluid is insufficient Loss of appetite occurs, and in the long term, general force, boredom, fatigue or mental anxiety occur, and rapid loss of salinity in the body causes obvious physical and mental loss such as dizziness, indifference, unconsciousness, and deprivation.

On the other hand, human skin color is determined by the concentration and distribution of melanin in the skin, in addition to genetic factors are also affected by environmental conditions such as ultraviolet rays, physiological conditions such as fatigue, stress.

Melanin (black pigment, Melanin) is a representative pigment that plays an important role in showing the color of human skin, protecting the skin, and preventing skin damage caused by ultraviolet rays (UV). The melanin is produced by melanocyte cells in the basal layer of the epidermis of the skin. Melanin is produced by non-enzymatic oxidation after tyrosinase acts on tyrosine, a kind of amino acid, into dopa and dopaquinone. Although such a melanogenesis pathway is known, the mechanism of inducing melanin synthesis, the previous step in which tyrosinase works, is not yet clear. The tyrosinase (EC: 1.14.18.1) is one of a copper-containing monooxygenase containing a copper atom, as described above, the melanin biosynthesis pathway It is an important enzyme in the (key enzyme).

When the melanin is excessively produced in the skin, it may darken the skin color and may cause spots, freckles, and the like. Therefore, by inhibiting the synthesis of melanin pigment in the skin can not only brighten the skin color to achieve skin whitening, but also improve the hyperpigmentation of skin such as blemishes, freckles and the like caused by ultraviolet rays, hormones and genetic causes.

Reduction of the biosynthesis of the melanin pigment may be performed by inhibiting tyrosinase, reducing exposure to ultraviolet (UV), or inhibiting melanocytes. Among the above methods, since tyrosinase is the safest method, the tyrosinase inhibitor is widely used as a cosmetic for whitening.

Conventionally, a substance having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, glutathione, etc., which is a chemical component, is mixed with cosmetics such as ointments, creams, and essences for skin to realize skin whitening, blemishes, freckles, etc. Improved skin hyperpigmentation.

However, hydroquinone has severe skin irritation and has a problem of limiting the amount of the compound to a very small amount. Ascorbic acid is easily oxidized, and the cosmetics containing the compound may cause discoloration and discoloration, resulting in deterioration of product quality and thus whitening effect. It has a downside. In addition, kojic acid is more likely to be carcinogenic when administered orally, and further safety verification is required, and thiol compounds such as glutathione and cysteine not only have a characteristic unpleasant odor, but also have problems with transdermal absorption, and glycosides and derivatives thereof. Also has a high polarity is difficult to use as a component of the cosmetic composition.

In addition, the material having a tyrosinase inhibitory activity isolated from the existing natural material is mainly a phenol structure (metal structure) or a metal chelating agent (metal chelating agent), the material having the inhibitory activity Nevertheless, its use is limited due to its high toxicity and insufficient penetrative ability.

The present invention is to improve the problems of the prior art as described above, it is an object of the present invention to provide an effective ingredient of the skin whitening composition containing a natural substance which is effective in whitening the skin and has little side effects.

In order to achieve the above object, the present invention provides a method for preparing sun salt having whitening activity and a sun salt having excellent whitening activity prepared by the method.

In addition, in order to achieve the above object, the present invention provides a whitening cosmetic composition comprising the salt excellent in whitening activity as an active ingredient.

The inventors of the present invention while studying a material having a whitening activity derived from a natural material without problems of stability, such as side effects or resistance, when salt and bamboo are mixed in a specific ratio, and heated by a specific method, the salt having a whitening activity It was confirmed that can be prepared, to complete the present invention.

Hereinafter, the present invention will be described in more detail.

In the present invention, salt means all types of salt that can be used for food, not for industrial purposes, and include salt, rock salt, flower salt, roasted salt, bamboo salt and refined salt or refined salt, molten salt, refined salt, processed salt, and the like. And, preferably, sun salt or purified salt.

In the present invention, the natural salt refers to an unprocessed salt made by concentrating seawater by evaporating water using natural forces such as wind and sunlight after introducing seawater or seawater into a reservoir or salt field, and having a thick and translucent hexagon. Has the characteristic of having a crystal. The sun salt is called mineral salt because it is rich in many minerals (minerals) such as calcium, magnesium, zinc, potassium, and iron.

The present invention provides a salt whitening function, and provides a method for preparing a salt having a whitening activity.

The method for preparing salt having whitening activity includes a) a first heat treatment step of heat treating salt; b) a second heat treatment step of heat-treating the first heat-treated salt; c) mixing the second heat-treated salt and bamboo; d) a third heat treatment step of performing heat treatment while raising the temperature of the second heat-treated salt and bamboo mixture, and d) a fourth heat treatment step of maintaining the temperature of the mixture on which the third heat treatment step is performed. have.

The salt may preferably be a natural salt in which the whitening activity is most improved through heat treatment.

In the present invention, the natural salt refers to an unprocessed salt made by concentrating seawater by evaporating water using natural forces such as wind and sunlight after introducing seawater or seawater into a reservoir or salt field, and having coarse and translucent hexagons. Has the characteristic of having a crystal.

The sun salt preferably has a sodium chloride content of 75 to 95% by weight, a calcium content of 0.1 to 0.3% by weight, a potassium content of 0.15 to 0.45% by weight, and a magnesium content of 0.4 to 2.0% by weight based on the total weight of the sun salt. %, Preferably sodium chloride content of 80 to 90% by weight, calcium content of 0.11 to 0.22% by weight, potassium content of 0.2 to 0.35% by weight, magnesium content of 0.6 to 1.5% by weight, and , More preferably, sodium chloride content of 82.5 to 88% by weight, calcium content of 0.13 to 0.18% by weight, potassium content of 0.23 to 0.27% by weight, magnesium content of 0.8 to 1% by weight, further manganese (Mn), iron (Fe), copper (Cu), zinc (Zn) and strontium (Sr) may be included.

The sodium chloride content may be 82.5 to 88% by weight, preferably 83 to 87% by weight, more preferably 84 to 86% by weight, the calcium content may be 0.13 to 0.18% by weight, preferably 0.14 to 0.16% by weight The potassium content may be 0.23 to 0.27% by weight, preferably 0.24 to 0.26% by weight, more preferably 0.245 to 0.25% by weight, and the magnesium content is 0.8 to 1% by weight, preferably 0.81 to 0.9% by weight, more Preferably 0.82 to 0.84% by weight.

The first heat treatment step a) of heat treating the salt comprises salt, preferably sun salt, at 700 to 900 ° C, preferably at 750 to 850 ° C for 30 minutes to 4 hours, preferably 45 minutes to 2 hours, more preferably. May be to heat or bake for 60 to 90 minutes, the heat treatment may be performed once.

B) the second heat treatment step of heat-treating the salt is 1 hour to 5 hours, preferably 1 hour 30 minutes to 4 hours at 700 to 900 ℃, preferably 750 to 850 ℃ the first heat-treated salt, More preferably, heat or baking may be performed for 2 to 3 hours, and the heat treatment may be performed once.

The step c) of mixing the second heat-treated salt and bamboo may be performed by mixing the salt and the bamboo, which have been subjected to the second heat treatment, in a weight ratio of 40:60 to 70:30 (sun salt: bamboo). have.

The bamboo is a generic name of an evergreen perennial plant belonging to a monocotyledonous plant rice plant and bamboo subfamily, preferably, the maturity of bamboo may be three to four years old. The bamboo is preferably cut off and used immediately because the sap is insufficient when one week or more. For example, the bamboo may be to use the form of a chip (chip) after crushing the bamboo maturity of 3 to 4 years old. The bamboo chip may have a size of 1 cm × 2 cm, in consideration of the degree of mixing with salt, productivity and process cost.

C) mixing the heat-treated natural salt and bamboo is performed by mixing the natural salt and bamboo at a weight ratio of 40:60 to 70:30 (salt: bamboo), preferably 50:50 to 60:40. can do.

In the case of mixing the natural salt and bamboo, it is remarkably superior to the mixture of bamboo and MgSO ₄ mixed together or the mixture of Banbusae Caulis and MgSO ₄ , which is usually added when preparing the roasted salt. It was confirmed that there is tyrosinase inhibitory activity.

D) performing a third heat treatment step of heat treatment while raising the temperature of the heat-treated salt and bamboo mixture is heated for 4 to 6 hours, preferably 4 to 30 minutes to 5 hours and 30 minutes. By the heat treatment until the mixture is 1,100. The third heat treatment process may be performed by the method described in FIG. 1 as an example. Specifically, the salt and bamboo mixture is heated to increase 2 per minute until 200, and heat is increased to 4 per minute until 200 to 400, and 4 per minute until 400 to 1,100. This can be done by applying heat. More specifically, by the above method, in order for the salt and bamboo mixture to be heated from 0 to 200, 1 hour 20 minutes to 2 hours may elapse, and in order for the mixture to be heated from 200 to 400, 30 Minutes to 1 hour 10 minutes may elapse, and 2 hours 30 minutes to 3 hours 10 minutes may elapse for the mixture to heat from 400 to 1,100.

The step e) of performing the fourth heat treatment step of performing heat treatment while maintaining the temperature of the mixture in which the third heat treatment step is performed is performed at 1,100 to 15 minutes to 60 minutes, preferably 20 It may be carried out by a method of heat treatment for minutes to 40 minutes. Step d), the third heat treatment process, may be performed continuously with step e).

In the case of performing the heat treatment process, unlike the conventional method of manufacturing the same bamboo salt repeating the same process several times, by performing the entire heating process, in relation to the whitening effect that the existing salt does not have or only limited Not only can we produce excellent functional salts with remarkably excellent whitening effect, but also the process cost is simpler compared to the existing processes such as melting and salting after heating to 1,200 to 1,300 and solidifying and pulverizing. There is an advantage that the manufacturing cost is reduced.

The present invention also provides a salt having a whitening effect prepared by the above production method. The salt prepared by the above production method was expected to have excellent tyrosinase inhibitory activity and excellent whitening effect.

In general, tyrosinase is a polyphenol oxidase widely distributed in the flora and fauna and catalyzes two reactions in the body. One function is tyrosinase oxidase, which oxidizes monophenol to o-diphenol, and the other function as dopa oxidase, which oxidizes o-diphenol to o-quinone. Tyrosinase plays an important role in the biosynthesis of melanin polymers in the body due to its function as an oxidase, and particularly plays a crucial role in producing melanin in melanocytes. The melanin appears on the skin as lesions such as blemishes, freckles, melanin hyperpigmentation and the like. Therefore, when the tyrosinase inhibitory activity is excellent, it is expected to inhibit melanin production and excellent whitening effect.

The present invention also provides a cosmetic composition with a whitening activity comprising a salt having a whitening activity prepared by the above production method as an active ingredient.

The cosmetic composition with a whitening activity of the present invention is a salt having the above whitening activity as an active ingredient, the active ingredient is 0.001 to 99% by weight, preferably 0.1 to 90% by weight, more preferably based on the total composition Preferably 1 to 50% by weight may be included. That is, the content of the active ingredient is preferably above the minimum value so as to achieve the minimum skin lightening effect, and the content of the active ingredient is preferably below the maximum value in consideration of deterioration of the feeling of use due to excessive addition and applicability to various formulations. Do. At this time, the content of the active ingredient is preferably adjusted appropriately within the range according to the content of the components contained in the formulation or the whitening composition.

The whitening composition may be made of a skin contact material in contact with the skin, such as cosmetics, detergents and fibers. In addition, the whitening composition is not limited to the route of administration, for the purpose of whitening, topical administration such as transdermal administration, subcutaneous administration is preferred.

The cosmetic composition with the whitening activity may be further formulated with an active ingredient as necessary, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. Can be. In addition, the cosmetic composition with the whitening activity is used for the skin as a softening longevity (skin), astringent longevity, nourishing longevity (lotion), nutrition cream, massage cream, essence, pack, powder, ointment, suspension, emulsion, spray, It may be prepared in a formulation such as cosmetic liquid, soap, shampoo, skin patch, or skin gel, and may include various conventional carriers and additives suitable for each of these formulations and well known in the art. The admixable excipients include, but are not limited to, glycerin, starch, lactose, water, alcohol, propylene glycol, salicylic acid, dahyroacetic acid and saline.

In addition, the cosmetic composition with a whitening activity of the present invention may be provided for the use of basic cosmetics, makeup cosmetics, body cosmetics, scalp cosmetics or shaving cosmetics. Examples of the basic cosmetics include creams, lotions, packs, massage creams, emulsions, etc. The makeup cosmetics include foundation, makeup base, lipstick, eyeshadow, eyeliner, mascara, eyebrow pencil, and the like. Examples include soaps, liquid detergents, baths, sunscreen creams and sun oils, and hair tonics and scalp treatments include scalp cosmetics, and aftershave and shaving creams. .

As described above, in the case of the present invention, the manufacturing process is simple and can significantly improve the tyrosinase inhibitory activity of the salt in an economical manner as compared to the existing method, and the salt prepared by the method As well as excellent cinnase inhibitory activity, and also excellent antioxidant capacity, the salt provided by the method can be applied to a variety of whitening cosmetic compositions or whitening compositions that require a whitening function, having a conventional whitening activity Unlike the substance, the cosmetic composition of the present invention does not cause problems such as side effects by using salt, which is a natural substance recently recognized as a food, as an active ingredient, and furthermore, natural salt produced in abundance in the west coast of Korea, especially Jeollanam-do, can be used as a raw material. Therefore, it is economically advantageous and the effect is very large industrially.

Hereinafter, preferred embodiments of the present invention will be described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1 Preparation of Functional Salt

Solar salts used in the experiments were purchased from Taepyung Salt (Korea), refined salts were purchased from Hangeum (Korea), and NaCl (PS, Purified salts) was S9625 (Sigma, USA, NaCl 99.5%). Was purchased and used, purple bamboo salt (PBS) and key power salt (BS, Bamboo salt) was purchased from fresh water (Korea).

Among the subsidiary materials used to manufacture functional salts with improved whitening activity, bamboo was purchased from plastic & bamboo, and MgSO ₄ and bamboo were purchased from Farm Maker. Functional salts with improved antioxidant capacity are processed salts prepared using reagent grade salts according to the raw materials, Processed purified salts (P-PS) and Processed solar salts (P-SS) for functional salts manufactured using sun salt. ) And functional salts prepared using baked salt through one heat treatment, respectively, were named P-RS (Processed Roated salts). The one-time heat treatment means baking the salt at 800 ° C. for 1 to 2 hours.

Preparation of the functional salt corresponding to the present invention is carried out by a method of heat-treating the salt divided into two times. The heat treatment corresponding to the pretreatment of the salt was carried out by heat-treating the salt at 800 ° C. for 2 hours. Heat treatment corresponding to the main treatment of the salt was performed by applying a heat treatment under the conditions of FIG. 1 and Table 1 below.

Addition of subsidiary materials to confirm the optimum condition of functional salts with whitening activity is 65: 25: 10 (salt: MgSO ₄₎ : Mixed), and in the case of functional salt 2, 60: 40 (salt: bamboo) based on the weight part, and in the case of functional salt 3, 60: 30: 10 (salt: bamboo: MgSO ₄ ).

TABLE 1

Step Temp (° C) Step duration
in h: min Temp-min
(℃ / min ) Temp gradient type One 200 01:40 2 Gradient 2 400 00:50 4 Gradient 3 1,100 02:55 4 Gradient 4 1,100 00:30 - Step -n- hold

TABLE 2

More specifically, each mixture of the raw material salt and the subsidiary material was added to a ceramic container made of alumina, and prepared by heating in an electric furnace (electric heating, Korea) for the times shown in Table 1 and FIG. 1.

Example  2: Whitening  Measure

Tyrosinase is a polyphenol oxidase widely distributed in the flora and fauna, and plays a crucial role in producing melanin in melanocytes. The melanin appears on the skin as lesions such as blemishes, freckles, melanin hyperpigmentation and the like.

In the above example, the salt prepared in Example 1 was used as a sample, and the concentration of DOPA oxidase was measured using tyrosinase (Sigma, USA) isolated from mushrooms. More specifically, 2 ml of tyrosinase (110 U / ml) was added to a mixture of 0.2 ml of substrate solution dissolved in 10 mM L-DOPA in 0.5 ml of phosphate buffer (0.175M, pH 6.8) and 0.1 ml of sample solution, and 25 ° C. The reaction mixture was reacted for 2 minutes at, and the absorbance at 475 nm was measured using a spectrophotometer (Spectrophotometer, Beckman DU-7500) in the reaction solution, and the results are shown in FIG. 1. Calculation of tyrosinase inhibitory activity is shown in the following formula 1, that is, the absorbance decrease rate of the sample solution addition group and no addition group.

[Calculation 1]

As shown in FIG. 1, PRS-2, PSS-2, and PPS-2, which were subjected to salt processing, were found to be the best, and among the salts processed by adding only bamboo to heat treatment, sun salt When PRS-2 treated with the previous step, which is the primary baking step, added 0.35 mg, the inhibitory activity was nearly 10 times higher than that of other salts, and the tyrosinase inhibitory activity was confirmed to be the best.

The excellent tyrosinase inhibitory activity as a result can be interpreted to be the most effective blocking the synthesis of melanin in the body, it was confirmed that the whitening effect is the best.

1 is a graph showing a heat treatment process of a method for improving the whitening activity of salt according to an embodiment of the present invention.

Figure 2 is a graph measuring the tyrosinase inhibitory activity according to an embodiment of the present invention.

Claims (4)

a) a first heat treatment step of heat-treating salt at 700 to 900 ° C. for 30 minutes to 4 hours; b) a second heat treatment step of heat-treating the salt subjected to the first heat treatment at 700 to 900 ° C. for 1 hour to 5 hours; c) mixing the salt and the bamboo subjected to the second heat treatment to 40:60 to 70:30 (natural salt: bamboo) based on the weight ratio; d) a third heat treatment step of heating the salt and bamboo mixture for 4 to 6 hours to heat the mixture until 1,100 ° C .; And e) a fourth heat treatment step of heat-treating the mixture on which the third heat treatment step is performed at 1,100 ° C. for 15 to 60 minutes Method for producing a salt having a whitening activity comprising a. The method of claim 1, In step d), heat is applied so that the temperature of the mixture is increased by 2 per minute when the temperature of the mixture is in the range of 0 to 200, and when the temperature of the mixture is in the range of 200 to 400, the temperature of the mixture is increased by 4 per minute. Heat is applied to the method of producing a salt having a whitening activity that is carried out by applying a heat so that 4 per minute in the temperature range of the mixture from 400 to 1,100. A salt having a whitening activity prepared by the method of any one of claims 1 and 2. A cosmetic composition having a whitening activity comprising the salt of claim 3 as an active ingredient.

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